pycom07g14800

Clustered mitochondria protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007431
Physical Location & Seq
Forward (+)
16843364 .. 16853598
10235 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5391 bp
ATGGCGCCCAGAAACAGCCGTGGAAAGGGAAAAGGAGACAAGAAGAAGAAAGAAGAGAAGGTTCTTCCTGTTGTTATGGACATCACTGTGAAACTTCCTGATGAAAGCAGTGTCGTTTTGAAGGTAAGAGGGCATCGATTAAATGACGCGGCGGACGTCTCCGCACTCAAGCCATGCGTCCTGACGTTGGTCGAAGAGGACTACGATGAACAGCGCGCCACGGCCCACGTTAGACGCGTGCTCGACATCGTTGCATGCACGACGAGCTTCGGAGCGTCGTCTTTGCCGGCGAAGGACCAAAGCCCTAAGCTCGATGCTTCTTCAACTGGCTCTGGTAAAAATGCGCCGGGTGCGCAGGATAAGAGCTTCAAGAAATCCAACGCCACGGCCGCCGCAGCCAACGCCTCCAAGTCTCAAGTTTCTACCGGAACCGATAAGCGAGACGTGGCGGTGGACTCCGAGACCGAGATGAGCCACTCCTGCCTCAAGCTTGGTTCTTTCTACGACTTCTTCTCTCTCTCCCACCTCACACCCCCTTTGCAATTTGTTCGAAGGGCGGCGACACGGCAAGTCGATGAGATTGAGGCGGATGATCACCTTTTTTCTCTTGAAGTGAAGCTTTGCAATGGGAAGGTGGTTCTCGTTGAAGCTTGCCAGAAGGGGTTTTATAGCGTAGGGAAGCGGCGGATTCTGTGTCACAACCTCGTCGATTTGCTACGGCAGCTTAGTAGAGCCTTTGACAATGCTTATGATGAGCTCTTGAAAGCATTTTCGGAACGGAATAAGTTTGGGAATCTTCCTTATGGGTTTAGAGCAAACACGTGGCTCGTACCGCCTGTTTCAGCTCAGTCGCCTTCAGTTTTCCCTGCTCTCCCCATGGAGGATGAAACTTGGGGTGGAAATGGAGGTGGTCTAGGAAGAGATGGGAAATTTGATCTAATCCCCTGGGCTAATGAGTTTTGGCATATTGCATCCATGCCTTGCAAGACAGCTGAGGAGAGGCAGATTCGTGATGGGAAGGCATTCCTTCTTCACAGCCTATTCGTCGATTTTTCCATTTGTAGAGCCATTAATGCTGTGCAGCGTGTAATGGGAAAACCGGAATTGACTTTTTCAATTCCAAATAGTGAGATTCTTTACTCCGAGATTGTAGGGGACTTGAATGTCACAGTTATGAAAGATGTTACTAATGCAAGCTTTAAGGTAGATACTAAAATTGATGGAATTCAAGCAACTGGAGTTGATAAAGTGAATCTAGCACAACGAAACCTTCTGAAAGGGATCACTGCAGATGAAAATACTGCTGCCCACGATGTCAACACTCTAGGTGTTGTCAATGTCAGATATTGTGGTTACATTGCAGTTGTCAAAGTTGAGGGTAAAGAAAATAAGAAAGTCGGTTCTCCATCTCAGAGCATTGTATTTGTTGATCAACCCGAAGGTGGTGCTAATGCCCTTAATATCAACAGTTTAAGATTACTACTTCACAAGACAATACCTTCAGAGCAGAATAAACCGGCATCACATATGCAAACTCTGGAACATGAAGATCGATCACGATCAATCGGAGTTGTTGGGATGCCTCACAAAGATAAGAAACCGTCGAACGAGAAGGGTAAAAATGATATGAAGGTGGAGGGACTTGGGACGCCTCTTAAATCCCTTAAGAACAACAAGAAAAAATCAGATGGAGGCAATACCAAGCTGCAATCTGAAAGCTCAAAATCTCATGCAGATGGTGGTGTTGCGGAAGCTGAAAATACTATATCCCCTTCTGTAGAATCAAAGCTTGAGACTAATGCCAAAGAAAATGAGCATGTTCTGACAGAGATATTGTCTGATGCTGCCTTTGCTCGACTAAAAGATTCAGAAACTGGACTTCACTGCAAGTCTCTGCAAGAACTGATCGACTTGTCTCAGAAGTACTACTCTGAAGTTGCTCTTCCCAAACTGGTAGCAGATTTTGGTTCCTTGGAACTTTCACCAGTTGATGGTCGGACCTTGACTGATTTCATGCATACTAGAGGTCTTCGGATGCGCTCTCTGGGGCATGTTGTCAAGCTTTCGGAGAAGCTATCCCATGTGCAGTCCCTTTGCATTCATGAGATGATAGTGCGAGCTTTTAAGCACATACTTCAGGCAGTGATTGCCGCCGTTGGTAGCACTGAGAAGATGGCTGTATCAATTGCTGCTGCATTGAATCTTATGCTGGGCGTTTTGGATAATGAAGAATTAAATAGGTCTTGCGGTGTTCATTCTCTAGTGTGGAAGTGGCTGGAGGTATTCTTGCAAAAAAGATACGGATGGGATCTTAACAGCTTCAACTATGAAGATGTGAGGAGATTTGCAATTCTGCGTGGATTATGTCACAAGGTGGGTATTGAGATGGTTCCAAGGGATTTTGATATGGATTCTCCAAATCCATTCCAAAGTTCAGATATTGTCAGCCTAGTCCCAGTGCATAAGGTGACGTTTGGCATTAGAAGCATCTATGGTAGGCAACTCCTAGAATCATCAAAAACAGCTTTAGATAAGGGAAAACTTGAAGATGCAGTTGCATTTGGCACTAAGGCGCTTGCAAAGCTGGTAGCAGTTTGTGGTCCATACCATCGAATGACAGCCGGAGCTTACAGCCTCCTTGCTGTAGTTTTATATCACACTGGAGACTTTAATCAGGCTACAATTTATCAGCAAAAGGCCTTGGATATCAACGAGAGAGAATTAGGGCTTGACCATCCAGACACCATGAAGAGTTATGGGGATCTTGCTGTGTTCTATTACAGACTTCAACATACGGAGCTGGCTCTCAAGTATGTAAAGCGTGCTTTGTATCTTTTGCATCTCACATGTGGACCATCTCATCCAAACACTGCTGCAACATATATCAATGTGGCTATGATGGAGGAAGGCCTGGGAAATGTGCATGTTGCCCTCAGATATCTACACAAAGCTTTGAAGTGTAATCAAAGGTTACTCGGCCCAGACCATATTCAGACAGCAGCCAGTTACCACGCTATTGCAATTGCTCTCTCTTTGATGGAAGCTTACCCTTTAAGTGTTCAGCATGAGCAGACAACTTTGCAAATTCTGCGAGCAAAGCTTGGCCCAGATGACTTACGAACACAAGATGCTGCTGCTTGGCTTGAGTACTTTGAGTCCAAGGCTTTTGAACAGCAAGAAGCTGCCAGAAATGGGACTCGAAAGCCTGATGCATCTATTGCCAGCAAAGGCCACTTAAGTGTATCAGATTTGCTTGACTACATTAGTCCCACCAAAGGGAGAGAGGTTGCAGGAAAGAGGAAAAGCTACTTAACGAAGTTGAAGGAAAAATCCATCCAAACTGTCAGCTCAGCTAGTTCCGATGAGTCTTCAAAAGAAACCACAAAAGAGGGTTCTGATGAAGAGGGTCAGGAGACAAATGTACTTGAACCATGTGATAGAACAGATGTAATCCAGGAGAACAGCTCTGCTCCGGTTGACCCTCAGCATGCCGTGGAAGAGATTGCAGAGGAAAATTCAAATGTTTTCGATCAGATACCTGCTGAAACATATATCGATGGTGGTGACGATGGCTGGCAATCAGTTCACAGACCTAGATCAGCTGGCTCATATGGGCGGCGTCTTAGGCAGTGGCGGGCAACTATTGGGAAAGTCTATAATTACCAGAAGAAATATGGTGAAAGTGACATGGACTGTTCTTCAGTGAAGAATACAAATCAAAACAGTAGGTATTATCTTGTAAAGAAAAGGCCAACATCTCATGGAAGTTATGCAGATAATCACACTGCAACCCCCTCCCAAGGTACAAAGTTTGGACGGAGAATAGTTAAAGGTGTAACATACAGAGTCAAGTCTATGCCTTCGTCTACCAAAGTAGATACTGAAGAGCCCTCTAATGGTAGCACAAGTTTGAGTTCTCCATCAGAACTCAGTCAAAATGTTTCACCACATGGAATTGGCCCAGTGAAAAATTCTGTAGTCAGTCTTGGGAAGTCTCCTTCTTACAAGGAAGTAGCATTGGCTCCACCAGGTACTGTTGGGAAATTTCAAACCCATAATAACATTCCAGATAATCAGGAACTTGGTGTCCGAGCGCAAGAAGAAGAAACAATTGAAGTCAAAGGAGATTCCAAACCAAACACAACTGAGTTAGAAAATATACTTGAAGAAAAGAACTCTGTGTTGGTTACGACGGACCACTTACCAGAGGAAACTGGAGCTGCTGAGAAGAAAGAAGAAGTTATTTCAAACGATGCAAAGGAAGATAAGTCTTCACTGATGGTATTTGAGAGTTTGGATGGACACGGATCCAGCGGTATTAAGGTTGATGAAGCGGTAGAAGATAACTTATTGACAGACGGAGTGCCAAAATCTTTGGGTTCTCCCAAGAAGGGAATCTGTGAGACGGATCCATCTGGCACTTGTGAACTCCGTGACTCCAACTCTACCACGCAAGGTGTAGCCGATGCTGTGAGTTCGGTTGATAATCGGGGATTGCCCAGTAAGAAGCTGTCTGCCTCAGCAGCTCCATTCAATCCCTCACCATCTGTTGCACGTGCTGCTTCGGTTTCCATGAACATTGCAATACCTTCGGGTGCAGGTAATGTCCCAATTGTTGCACCTTGGCCTGTAAACATGAATCTTCATCCAGGGCCTGGTACTGTCTTACCCACGGTCAATCCAATGTGCTCCTCCCCTCACCACCCATACCATTCTCCTCCTGCGACCCCGAACATCATACAACCATTGCCTTTTATGTACCCTCCTTACAGTCCACCCCAAGTAATCCGAACCAGTTCCTTTCCTGTTACTAGTAGCGGGTTTCATCCAAATCATTTTGCATGGCATAATGTGAACCCCAAAGCACCCCAGTTTGTTCATTGTACAGTTTGGCCTGGTTGTCATCCGATGGATTTCTCTGCCCCTACACCTGTTGCTGAGCCAATTTCTGAATCGACATTGGAGCCAAAATTTCACAGTGATGATTCTGCTCCAGTTCTGCCAGCGGACATTGACAATGTGGAGGAAACTAAGCAAGAAGTGAACCTTCTGAAATCAGAGGCAATGAGCAATGCAGTTGAAAGTGTAGAGGAAAATGGTCCTTCAAACCTTTGCGGGGTTGAACATGCTCAGAGTGAACCAAGTGAGAACCCAAATGGAAATGCTGCAAGTAGCGGTGAACTGACAAACGATGGAGAGAAAACCTTCAGCATTTTGATAAGGGGTAGGAGAAACCGAAAGGCGACTCTGCGAATGCCAATCAGTTTGCTCAGTCGACCGTATGGCTCACAGTCCTTCAAAGTCACATGTAACAGAGTAGTCAGAGGGAATGATGCTACCAAAGTCACCGGTTTTTCATCGAGTGAACGTTGCACAGCTACTGCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU_N TPR_12 eIF3_p135

Protein Analysis

1797

Amino Acids

196.01

Weight (kDa)

6.46

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_p135 PF12807 660 - 802 2.9e-23 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 843 - 905 6.6e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 846 - 943 1.9e-07 Nephrocystin-3 TPR domain
TPR_10 PF13374 873 - 914 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 873 - 943 1.4e-12 Tetratricopeptide repeat
TPR_10 PF13374 957 - 997 1.6e-07 Tetratricopeptide repeat
TPR_12 PF13424 957 - 1031 4.1e-12 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1163
AatII GACGTC 1 cut(s) 159
Acc16I TGCGCA 1 cut(s) 354
Acc36I ACCTGC 2 cut(s) 3547, 4589
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 2 cut(s) 1991, 4655
AccI GTMKAC 2 cut(s) 3866, 5275
AccII CGCG 3 cut(s) 149, 216, 237
AclI AACGTT 1 cut(s) 5368
AclWI GGATC 7 cut(s) 1289, 2316, 2769, 4302, 4315, 4403, 4416
AcoI YGGCCR 1 cut(s) 387
AcsI RAATTY 7 cut(s) 929, 1224, 3084, 3514, 3970, 4043, 4970
AcuI CTGAAG 7 cut(s) 840, 1485, 1953, 2120, 3684, 3903, 5191
AcvI CACGTG 2 cut(s) 822, 4556
AcyI GRCGYC 4 cut(s) 5, 156, 1649, 3619
AdeI CACNNNGTG 1 cut(s) 2374
AfaI GTAC 9 cut(s) 831, 1925, 3149, 3423, 3805, 4033, 4660, 4760, 4885
AflII CTTAAG 2 cut(s) 1664, 3235
AflIII ACRYGT 4 cut(s) 235, 819, 2846, 5306
AgeI ACCGGT 1 cut(s) 5348
AhlI ACTAGT 1 cut(s) 4811
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 7 cut(s) 944, 2910, 3453, 4027, 4648, 4654, 4894
AjuI GAANNNNNNNTTGG 2 cut(s) 4166, 4198
AleI CACNNNNGTG 1 cut(s) 3237
AloI GAACNNNNNNTCC 2 cut(s) 4071, 4103
Alw21I GWGCWC 3 cut(s) 243, 759, 4691
AlwI GGATC 7 cut(s) 1289, 2316, 2769, 4302, 4315, 4403, 4416
AlwNI CAGNNNCTG 4 cut(s) 1874, 4034, 4655, 5381
ApoI RAATTY 7 cut(s) 929, 1224, 3084, 3514, 3970, 4043, 4970
AseI ATTAAT 1 cut(s) 1071
AsiGI ACCGGT 1 cut(s) 5348
Asp700I GAANNNNTTC 3 cut(s) 1022, 4795, 5204
AspLEI GCGC 8 cut(s) 7, 216, 218, 346, 355, 2040, 2575, 4096
AsuC2I CCSGG 1 cut(s) 348
AsuII TTCGAA 1 cut(s) 550
AvaII GGWCC 6 cut(s) 295, 1998, 2600, 2852, 4195, 5099
BamHI GGATCC 2 cut(s) 4307, 4408
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 759, 3891
BbrPI CACGTG 2 cut(s) 822, 4556
BbsI GAAGAC 3 cut(s) 2021, 3360, 4263
Bbv12I GWGCWC 3 cut(s) 243, 759, 4691
BbvCI CCTCAGC 3 cut(s) 993, 3483, 4519
BceAI ACGGC 7 cut(s) 3, 237, 402, 581, 734, 2138, 3476
BcgI CGANNNNNNTGC 8 cut(s) 233, 267, 530, 564, 1427, 1461, 4572, 4606
BciT130I CCWGG 7 cut(s) 946, 2912, 3455, 4029, 4650, 4656, 4896
BclI TGATCA 2 cut(s) 592, 1429
BcnI CCSGG 1 cut(s) 348
BcuI ACTAGT 1 cut(s) 4811
BfmI CTRYAG 4 cut(s) 1287, 1776, 2643, 3975
BfoI RGCGCY 2 cut(s) 8, 2576
BfrI CTTAAG 2 cut(s) 1664, 3235
BfuAI ACCTGC 2 cut(s) 3547, 4589
BlpI GCTNAGC 2 cut(s) 3349, 4938
BmcAI AGTACT 2 cut(s) 1925, 3149
Bme1390I CCNGG 8 cut(s) 348, 946, 2912, 3455, 4029, 4650, 4656, 4896
Bme18I GGWCC 6 cut(s) 295, 1998, 2600, 2852, 4195, 5099
BmgBI CACGTC 1 cut(s) 445
BmiI GGNNCC 8 cut(s) 6, 430, 1969, 2391, 4023, 4309, 4410, 4965
BmrFI CCNGG 8 cut(s) 348, 946, 2912, 3455, 4029, 4650, 4656, 4896
BmrI ACTGGG 4 cut(s) 2450, 3956, 4494, 4864
BmuI ACTGGG 4 cut(s) 2450, 3956, 4494, 4864
BoxI GACNNNNGTC 1 cut(s) 188
BpiI GAAGAC 3 cut(s) 2021, 3360, 4263
BplI GAGNNNNNCTC 2 cut(s) 3449, 3481
BpmI CTGGAG 5 cut(s) 1257, 2297, 2682, 4236, 4977
Bpu10I CCTNAGC 4 cut(s) 306, 993, 3483, 4519
Bpu1102I GCTNAGC 2 cut(s) 3349, 4938
Bpu14I TTCGAA 1 cut(s) 550
BpuEI CTTGAG 6 cut(s) 152, 399, 470, 1811, 2792, 3164
BpuMI CCSGG 1 cut(s) 348
Bsa29I ATCGAT 3 cut(s) 136, 1552, 3555
BsaAI YACGTR 2 cut(s) 822, 4556
BsaBI GATNNNNATC 1 cut(s) 1558
BsaHI GRCGYC 4 cut(s) 5, 156, 1649, 3619
BsaI GGTCTC 1 cut(s) 455
BsaWI WCCGGW 4 cut(s) 425, 1099, 3472, 5348
Bse118I RCCGGY 3 cut(s) 286, 1516, 5348
Bse3DI GCAATG 6 cut(s) 631, 1356, 4578, 4745, 5070, 5077
Bse8I GATNNNNATC 1 cut(s) 1558
BseBI CCWGG 7 cut(s) 946, 2912, 3455, 4029, 4650, 4656, 4896
BseCI ATCGAT 3 cut(s) 136, 1552, 3555
BseJI GATNNNNATC 1 cut(s) 1558
BseMI GCAATG 6 cut(s) 631, 1356, 4578, 4745, 5070, 5077
BsePI GCGCGC 1 cut(s) 214
BseRI GAGGAG 4 cut(s) 1010, 2353, 4681, 4707
BseX3I CGGCCG 1 cut(s) 387
BseYI CCCAGC 1 cut(s) 2209
BsgI GTGCAG 3 cut(s) 1100, 2105, 4617
Bsh1236I CGCG 3 cut(s) 149, 216, 237
Bsh1285I CGRYCG 2 cut(s) 390, 5279
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 5348
BshVI ATCGAT 3 cut(s) 136, 1552, 3555
BsiEI CGRYCG 2 cut(s) 390, 5279
BsiHKAI GWGCWC 3 cut(s) 243, 759, 4691
BsiSI CCGG 8 cut(s) 287, 347, 426, 1100, 1517, 2622, 3473, 5349
BslFI GGGAC 8 cut(s) 1169, 1653, 1660, 2074, 2438, 3208, 3252, 4592
BsmBI CGTCTC 3 cut(s) 163, 435, 4397
BsmFI GGGAC 8 cut(s) 1169, 1653, 1660, 2074, 2438, 3208, 3252, 4592
BsmI GAATGC 3 cut(s) 1022, 2097, 5259
Bso31I GGTCTC 1 cut(s) 455
Bsp119I TTCGAA 1 cut(s) 550
Bsp1286I GDGCHC 4 cut(s) 243, 759, 3891, 4691
Bsp1407I TGTACA 1 cut(s) 4883
Bsp1720I GCTNAGC 2 cut(s) 3349, 4938
Bsp19I CCATGG 1 cut(s) 876
BspDI ATCGAT 3 cut(s) 136, 1552, 3555
BspFNI CGCG 3 cut(s) 149, 216, 237
BspHI TCATGA 1 cut(s) 2101
BspLI GGNNCC 8 cut(s) 6, 430, 1969, 2391, 4023, 4309, 4410, 4965
BspMAI CTGCAG 1 cut(s) 1291
BspMI ACCTGC 2 cut(s) 3547, 4589
BspPI GGATC 7 cut(s) 1289, 2316, 2769, 4302, 4315, 4403, 4416
BspQI GCTCTTC 2 cut(s) 1947, 3879
BspT104I TTCGAA 1 cut(s) 550
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 2 cut(s) 1664, 3235
BspTNI GGTCTC 1 cut(s) 455
BsrDI GCAATG 6 cut(s) 631, 1356, 4578, 4745, 5070, 5077
BsrFI RCCGGY 3 cut(s) 286, 1516, 5348
BsrGI TGTACA 1 cut(s) 4883
BssAI RCCGGY 3 cut(s) 286, 1516, 5348
BssHII GCGCGC 1 cut(s) 214
BssNI GRCGYC 4 cut(s) 5, 156, 1649, 3619
BssT1I CCWWGG 7 cut(s) 876, 1971, 2393, 2700, 3159, 3799, 4622
Bst2UI CCWGG 7 cut(s) 946, 2912, 3455, 4029, 4650, 4656, 4896
Bst6I CTCTTC 8 cut(s) 48, 189, 913, 1947, 2744, 3396, 3492, 3879
BstACI GRCGYC 4 cut(s) 5, 156, 1649, 3619
BstAFI CTTAAG 2 cut(s) 1664, 3235
BstAPI GCANNNNNTGC 4 cut(s) 2147, 3088, 3218, 4559
BstAUI TGTACA 1 cut(s) 4883
BstBAI YACGTR 2 cut(s) 822, 4556
BstBI TTCGAA 1 cut(s) 550
BstDSI CCRYGG 6 cut(s) 19, 219, 384, 876, 3492, 4671
BstFNI CGCG 3 cut(s) 149, 216, 237
BstH2I RGCGCY 2 cut(s) 8, 2576
BstHHI GCGC 8 cut(s) 7, 216, 218, 346, 355, 2040, 2575, 4096
BstMCI CGRYCG 2 cut(s) 390, 5279
BstNI CCWGG 7 cut(s) 946, 2912, 3455, 4029, 4650, 4656, 4896
BstNSI RCATGY 8 cut(s) 258, 1820, 2054, 2850, 2927, 3491, 5129, 5310
BstPAI GACNNNNGTC 1 cut(s) 188
BstSCI CCNGG 8 cut(s) 346, 944, 2910, 3453, 4027, 4648, 4654, 4894
BstSFI CTRYAG 4 cut(s) 1287, 1776, 2643, 3975
BstUI CGCG 3 cut(s) 149, 216, 237
BstV2I GAAGAC 3 cut(s) 2021, 3360, 4263
BstX2I RGATCY 4 cut(s) 2308, 2761, 4307, 4408
BstYI RGATCY 4 cut(s) 2308, 2761, 4307, 4408
BstZI CGGCCG 1 cut(s) 387
Bsu15I ATCGAT 3 cut(s) 136, 1552, 3555
BsuTUI ATCGAT 3 cut(s) 136, 1552, 3555
BtgI CCRYGG 6 cut(s) 19, 219, 384, 876, 3492, 4671
BtrI CACGTC 1 cut(s) 445
BtsI GCAGTG 7 cut(s) 115, 1284, 1882, 2148, 2868, 3635, 3783
BveI ACCTGC 2 cut(s) 3547, 4589
CaiI CAGNNNCTG 4 cut(s) 1874, 4034, 4655, 5381
CciI TCATGA 1 cut(s) 2101
CfoI GCGC 8 cut(s) 7, 216, 218, 346, 355, 2040, 2575, 4096
Cfr10I RCCGGY 3 cut(s) 286, 1516, 5348
ClaI ATCGAT 3 cut(s) 136, 1552, 3555
CseI GACGC 6 cut(s) 155, 166, 243, 264, 1657, 3608
CsiI ACCWGGT 1 cut(s) 4027
Csp6I GTAC 9 cut(s) 830, 1924, 3148, 3422, 3804, 4032, 4659, 4759, 4884
CspAI ACCGGT 1 cut(s) 5348
CviQI GTAC 9 cut(s) 830, 1924, 3148, 3422, 3804, 4032, 4659, 4759, 4884
DinI GGCGCC 1 cut(s) 6
DraIII CACNNNGTG 1 cut(s) 2374
DrdI GACNNNNNNGTC 1 cut(s) 1163
DseDI GACNNNNNNGTC 1 cut(s) 1163
EaeI YGGCCR 1 cut(s) 387
EagI CGGCCG 1 cut(s) 387
Eam1104I CTCTTC 8 cut(s) 48, 189, 913, 1947, 2744, 3396, 3492, 3879
EarI CTCTTC 8 cut(s) 48, 189, 913, 1947, 2744, 3396, 3492, 3879
EciI GGCGGA 3 cut(s) 167, 602, 700
Ecl136II GAGCTC 1 cut(s) 757
EclXI CGGCCG 1 cut(s) 387
Eco130I CCWWGG 7 cut(s) 876, 1971, 2393, 2700, 3159, 3799, 4622
Eco147I AGGCCT 2 cut(s) 2699, 2910
Eco24I GRGCYC 2 cut(s) 759, 3891
Eco31I GGTCTC 1 cut(s) 455
Eco32I GATATC 2 cut(s) 2707, 2939
Eco47I GGWCC 6 cut(s) 295, 1998, 2600, 2852, 4195, 5099
Eco52I CGGCCG 1 cut(s) 387
Eco53kI GAGCTC 1 cut(s) 757
Eco57I CTGAAG 7 cut(s) 840, 1485, 1953, 2120, 3684, 3903, 5191
Eco72I CACGTG 2 cut(s) 822, 4556
EcoICRI GAGCTC 1 cut(s) 757
EcoO109I RGGNCCY 1 cut(s) 4652
EcoRI GAATTC 1 cut(s) 1224
EcoRII CCWGG 7 cut(s) 944, 2910, 3453, 4027, 4648, 4654, 4894
EcoRV GATATC 2 cut(s) 2707, 2939
EcoT14I CCWWGG 7 cut(s) 876, 1971, 2393, 2700, 3159, 3799, 4622
EcoT22I ATGCAT 2 cut(s) 2019, 3214
EcoT38I GRGCYC 2 cut(s) 759, 3891
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 7 cut(s) 876, 1971, 2393, 2700, 3159, 3799, 4622
Esp3I CGTCTC 3 cut(s) 163, 435, 4397
FalI AAGNNNNNCTT 6 cut(s) 603, 635, 1926, 1958, 5031, 5063
FaqI GGGAC 8 cut(s) 1169, 1653, 1660, 2074, 2438, 3208, 3252, 4592
FauI CCCGC 3 cut(s) 3627, 4811, 5108
FauNDI CATATG 2 cut(s) 1527, 3610
FbaI TGATCA 2 cut(s) 592, 1429
FblI GTMKAC 2 cut(s) 3866, 5275
FriOI GRGCYC 2 cut(s) 759, 3891
FspI TGCGCA 1 cut(s) 354
GlaI GCGC 8 cut(s) 6, 215, 217, 345, 354, 2039, 2574, 4095
GsaI CCCAGC 1 cut(s) 2213
GsuI CTGGAG 5 cut(s) 1257, 2297, 2682, 4236, 4977
HaeII RGCGCY 2 cut(s) 8, 2576
HapII CCGG 8 cut(s) 287, 347, 426, 1100, 1517, 2622, 3473, 5349
HgaI GACGC 6 cut(s) 155, 166, 243, 264, 1657, 3608
HhaI GCGC 8 cut(s) 7, 216, 218, 346, 355, 2040, 2575, 4096
Hin1I GRCGYC 4 cut(s) 5, 156, 1649, 3619
Hin6I GCGC 8 cut(s) 5, 214, 216, 344, 353, 2038, 2573, 4094
HinP1I GCGC 8 cut(s) 5, 214, 216, 344, 353, 2038, 2573, 4094
HincII GTYRAC 3 cut(s) 1318, 3478, 5276
HindII GTYRAC 3 cut(s) 1318, 3478, 5276
HindIII AAGCTT 9 cut(s) 488, 617, 648, 1195, 1787, 2060, 2949, 3042, 3098
HpaII CCGG 8 cut(s) 287, 347, 426, 1100, 1517, 2622, 3473, 5349
Hpy99I CGWCG 6 cut(s) 265, 280, 710, 1049, 1606, 4195
HpyCH4IV ACGT 8 cut(s) 156, 185, 228, 444, 821, 2471, 4555, 5368
HpySE526I ACGT 8 cut(s) 156, 185, 228, 444, 821, 2471, 4555, 5368
Hsp92I GRCGYC 4 cut(s) 5, 156, 1649, 3619
HspAI GCGC 8 cut(s) 5, 214, 216, 344, 353, 2038, 2573, 4094
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 1 cut(s) 286
KroNI GCCGGC 1 cut(s) 288
Ksp22I TGATCA 2 cut(s) 592, 1429
LguI GCTCTTC 2 cut(s) 1947, 3879
MabI ACCWGGT 1 cut(s) 4027
MaeII ACGT 8 cut(s) 156, 185, 228, 444, 821, 2471, 4555, 5368
MfeI CAATTG 4 cut(s) 2184, 3021, 4110, 4611
MflI RGATCY 4 cut(s) 2308, 2761, 4307, 4408
MhlI GDGCHC 4 cut(s) 243, 759, 3891, 4691
MluI ACGCGT 1 cut(s) 235
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 7 cut(s) 449, 3164, 3190, 3374, 3855, 4430, 5239
MmeI TCCRAC 3 cut(s) 402, 1976, 4464
Mph1103I ATGCAT 2 cut(s) 2019, 3214
MroNI GCCGGC 1 cut(s) 286
MroXI GAANNNNTTC 3 cut(s) 1022, 4795, 5204
MslI CAYNNNNRTG 5 cut(s) 86, 1172, 1734, 3237, 4980
MspA1I CMGCKG 4 cut(s) 992, 3602, 4314, 5006
MspCI CTTAAG 2 cut(s) 1664, 3235
MspI CCGG 8 cut(s) 287, 347, 426, 1100, 1517, 2622, 3473, 5349
MspR9I CCNGG 8 cut(s) 348, 946, 2912, 3455, 4029, 4650, 4656, 4896
MunI CAATTG 4 cut(s) 2184, 3021, 4110, 4611
Mva1269I GAATGC 3 cut(s) 1022, 2097, 5259
MvaI CCWGG 7 cut(s) 946, 2912, 3455, 4029, 4650, 4656, 4896
MvnI CGCG 3 cut(s) 149, 216, 237
NaeI GCCGGC 1 cut(s) 288
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 348
NcoI CCATGG 1 cut(s) 876
NdeI CATATG 2 cut(s) 1527, 3610
NgoMIV GCCGGC 1 cut(s) 286
NlaIV GGNNCC 8 cut(s) 6, 430, 1969, 2391, 4023, 4309, 4410, 4965
NmeAIII GCCGAG 1 cut(s) 2955
NmuCI GTSAC 9 cut(s) 695, 1165, 2366, 2467, 3563, 3683, 4433, 5302, 5344
NsbI TGCGCA 1 cut(s) 354
NsiI ATGCAT 2 cut(s) 2019, 3214
NspI RCATGY 8 cut(s) 258, 1820, 2054, 2850, 2927, 3491, 5129, 5310
NspV TTCGAA 1 cut(s) 550
OliI CACNNNNGTG 1 cut(s) 3237
PaeI GCATGC 2 cut(s) 258, 3491
PagI TCATGA 1 cut(s) 2101
PasI CCCWGGG 1 cut(s) 945
PauI GCGCGC 1 cut(s) 214
PceI AGGCCT 2 cut(s) 2699, 2910
PciI ACATGT 2 cut(s) 2846, 5306
PciSI GCTCTTC 2 cut(s) 1947, 3879
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 234
PctI GAATGC 3 cut(s) 1022, 2097, 5259
PdiI GCCGGC 1 cut(s) 288
PdmI GAANNNNTTC 3 cut(s) 1022, 4795, 5204
PflMI CCANNNNNTGG 2 cut(s) 1991, 4655
PfoI TCCNGGA 1 cut(s) 3453
PinAI ACCGGT 1 cut(s) 5348
PleI GAGTC 7 cut(s) 449, 3163, 3190, 3373, 3854, 4430, 5239
PluTI GGCGCC 1 cut(s) 8
PmaCI CACGTG 2 cut(s) 822, 4556
PmlI CACGTG 2 cut(s) 822, 4556
PpsI GAGTC 7 cut(s) 449, 3163, 3190, 3373, 3854, 4430, 5239
Ppu21I YACGTR 2 cut(s) 822, 4556
PscI ACATGT 2 cut(s) 2846, 5306
PshAI GACNNNNGTC 1 cut(s) 188
PshBI ATTAAT 1 cut(s) 1071
Psp124BI GAGCTC 1 cut(s) 759
Psp1406I AACGTT 1 cut(s) 5368
Psp6I CCWGG 7 cut(s) 944, 2910, 3453, 4027, 4648, 4654, 4894
PspCI CACGTG 2 cut(s) 822, 4556
PspFI CCCAGC 1 cut(s) 2209
PspGI CCWGG 7 cut(s) 944, 2910, 3453, 4027, 4648, 4654, 4894
PspN4I GGNNCC 8 cut(s) 6, 430, 1969, 2391, 4023, 4309, 4410, 4965
PstI CTGCAG 1 cut(s) 1291
PstNI CAGNNNCTG 4 cut(s) 1874, 4034, 4655, 5381
PsuI RGATCY 4 cut(s) 2308, 2761, 4307, 4408
PteI GCGCGC 1 cut(s) 214
PvuII CAGCTG 2 cut(s) 992, 3602
RsaI GTAC 9 cut(s) 831, 1925, 3149, 3423, 3805, 4033, 4660, 4760, 4885
RsaNI GTAC 9 cut(s) 830, 1924, 3148, 3422, 3804, 4032, 4659, 4759, 4884
RseI CAYNNNNRTG 5 cut(s) 86, 1172, 1734, 3237, 4980
SacI GAGCTC 1 cut(s) 759
SalI GTCGAC 1 cut(s) 5274
SapI GCTCTTC 2 cut(s) 1947, 3879
ScaI AGTACT 2 cut(s) 1925, 3149
SchI GAGTC 7 cut(s) 449, 3164, 3190, 3374, 3855, 4430, 5239
ScrFI CCNGG 8 cut(s) 348, 946, 2912, 3455, 4029, 4650, 4656, 4896
SduI GDGCHC 4 cut(s) 243, 759, 3891, 4691
SexAI ACCWGGT 1 cut(s) 4027
SfcI CTRYAG 4 cut(s) 1287, 1776, 2643, 3975
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 550
SinI GGWCC 6 cut(s) 295, 1998, 2600, 2852, 4195, 5099
SmiMI CAYNNNNRTG 5 cut(s) 86, 1172, 1734, 3237, 4980
SmlI CTYRAG 8 cut(s) 167, 414, 485, 1664, 1790, 2807, 3143, 3235
SmoI CTYRAG 8 cut(s) 167, 414, 485, 1664, 1790, 2807, 3143, 3235
SpeI ACTAGT 1 cut(s) 4811
SphI GCATGC 2 cut(s) 258, 3491
SseBI AGGCCT 2 cut(s) 2699, 2910
SspDI GGCGCC 1 cut(s) 4
SstI GAGCTC 1 cut(s) 759
StuI AGGCCT 2 cut(s) 2699, 2910
StyD4I CCNGG 8 cut(s) 346, 944, 2910, 3453, 4027, 4648, 4654, 4894
StyI CCWWGG 7 cut(s) 876, 1971, 2393, 2700, 3159, 3799, 4622
TaiI ACGT 8 cut(s) 159, 188, 231, 447, 824, 2474, 4558, 5371
TaqII GACCGA 1 cut(s) 479
TatI WGTACW 4 cut(s) 1923, 3147, 3421, 4883
TauI GCSGC 7 cut(s) 152, 392, 395, 560, 685, 2153, 3619
TseFI GTSAC 9 cut(s) 695, 1165, 2366, 2467, 3563, 3683, 4433, 5302, 5344
Tsp45I GTSAC 9 cut(s) 695, 1165, 2366, 2467, 3563, 3683, 4433, 5302, 5344
TspGWI ACGGA 9 cut(s) 793, 2316, 2810, 3832, 4208, 4320, 4374, 4421, 4421
Van91I CCANNNNNTGG 2 cut(s) 1991, 4655
Vha464I CTTAAG 2 cut(s) 1664, 3235
VpaK11BI GGWCC 6 cut(s) 295, 1998, 2600, 2852, 4195, 5099
VspI ATTAAT 1 cut(s) 1071
XapI RAATTY 7 cut(s) 929, 1224, 3084, 3514, 3970, 4043, 4970
XceI RCATGY 8 cut(s) 258, 1820, 2054, 2850, 2927, 3491, 5129, 5310
XcmI CCANNNNNNNNNTGG 1 cut(s) 3671
XmiI GTMKAC 2 cut(s) 3866, 5275
XmnI GAANNNNTTC 3 cut(s) 1022, 4795, 5204
ZraI GACGTC 1 cut(s) 157
ZrmI AGTACT 2 cut(s) 1925, 3149
Zsp2I ATGCAT 2 cut(s) 2019, 3214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.