pycom07g15600

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006617
Physical Location & Seq
Forward (+)
18048906 .. 18058889
9984 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5793 bp
ATGACCAAGAAGCTCACTCGCTTCGAACCGCAACTCACCATGGAACTCGACCTTGACTCGTTCCTCAATTCGCACCTCTCCCTCTCCGACGAAGACGACGACGACGACCTTAGCTCCGTCCCCCACCGCACGATCGACGAAATCCTCAACGACACCGATTCGTCCGCCTCATCGTCCCCGTCCTCCTCCTCTATCCACCGCCTCAATTCCGATCCCAAACCACCGGAACCGCCCAACGACGCCGTATCGGTCCTCGTCGTTAAGTCCGAGGATGAAAGGGCGGTCCGACCGAGGTCCAATCTGTACAGTCGGGTCAAGTCCGGGGATTTATCGGAGGATCCGGCCGGGAGGGTTCCGAAGCCGTCTCCATGGTTGCTCGGAGGCATGAGAACGAATGCGAAGCCCGGGGCGGCCCTGGCTGCAGCGGCGGCGGCCTCGAGGTCGATGCCAACGCCGCACGCCGCGGCAATCAAGTCGAGGAGGAGCACCGTGAGCGGAGTTATCCAGAAGGTTCTTGAAAGTTCGGAACTGGATGAAAAATCCGAGGTGAGTTCCAATTCTACTGGTGATATGACTGCAATTGGTCCGGAAGTGCTTAGGACAAAATCAAACGAATTACAAGTGGATTTTGGGGATGAATTGTTGAGAGAAGGCGGGGTTTTGGGGAGCGAAAGCAAGTTAGAGCAGACTTTTCGAGTTGATCAATCTCAAGTTGATGAGGCGAGTGCTGGGAATGCTGTGGAAGAAGAGAAGAATGTAAGTAGTTTTGATGAGAATTTGACAAATTTAGACACAAATGATGTTAAAGATACGGAATTTAGTAAGAATGTTGAGGTCGTAGAGGAATGTAAACAAGAAATTCAAGACTTAGATGATAACAGTCCCCGTTCGAAAGAGAGTGATAGTGAGGATAATGATGGTGGTGGTGGTGGCAAGGATGATGGTGATGATGGTGGTGGTAAGGAGTCTGAGGATAACAATGGCGATATTGGTAACGATAGCGATATTGGTGATGACGATGAATTGGGTTCAATCACTCAACTCGTGGAGGAGAGAATTGGGGAATTGGAGAACAGGAGGATTAGTAAAAAATCTGAAAAGAAGTTGCGCAAGCCACTTGAAATCGCTGAAGAGCTAGAGAAGAAGCAAGCTTCTAATGCTTTGCATTGGGAAGAGGGTGCCGCTGCTCAACCCATGAGGCTTGAGGGTGTTCGGAGAGGCTCCACCACATTAGGATACTTCAATGTCGATGCTAATAATCCCATTACTCGGACCCTTTCATCTCCGGCGTTAAGGCGTGATCATGGCTCACCGCAAGTCTTGGCAGTTCACAATAATTACATTGCAATAGGAATGGGAAGAGGGTCTATTCTTGTTATTCCTAGCAAGTACTCTGCTCATACTGCTGACAGCATGGATGCAAAGATGCTAATTCTTGGGTTGCAAGGAGAACGGTCTTATGCTGCTGTAACTTCTATGTGCTTCAATCAGCAAGGGGACTTGCTCTTAGCTGGTTATGCTGATGGTCACATTACAGTTTGGGACGTGCAGAGGGCATCGGCTGCCAAAATAATTACTGGAGAACATACGGCTCCAGTTGTACATATGTTGTTTTTGGGGCAGGATTCTCAGGTTACACGTCAATTTAAGGCAGTTACCGGTGATAGTAAGGGTCTGGTATTATTACATTCTTCCTCGGTGGTTCCCCTGCTTAATAGGTTCTCGATTAAAACACAGTGCCTTCTTGATGGACAAAATACGGGCACAGTGCTGTCAGCTTCACCACTCCTGTTTGATGAATTCTTTGGAGGGGCTTCACTGTCTTCTCAGGGAAGTGGGGCAGTTTCAGGCAGCAGTATTGGTGGTATGATGGGGGGTGTGGTTGGTGGAGATGCAGGTTGGAAACTCTTCAATGAGGGATCTTCTTTGGTCGAAGAAGGTGTGGTCGTGTTTGTCACCCATCACACTGTTCTGGTGGTAAGACTAACTCCCACTTTGGAAGTGTATGCTCGACTTTCCAAACCAGATGGGGTTCGGGAGGGTTCTATGCCTTGTACTGCTTGGAAATGTACAATACAGTCACACTGTTCACCTGCTAGTAGCGAAAATATGCCTGCTGAAGTAGTTGAAAGAGTTTCGTTACTTGCACTTGCTTGGGATCGAAAAGTTCTGGTGGCAAAGTTGGTCAAATCAGAGCTGAAAGTATATGGGAAATGGTCTCTTGAAAGTGCAGCCATAGGTGTGGCTTGGCTAGATGATCAGATGCTGGTGGTTCTCACGGTGACTGGACAACTTTGTTTGTTTGCAAAGGATGGAACTGTGATTCACCAAACAAGTTTTTCTGTTGATGGTTTTGGAGGGGATGATCTCATTGCTTATCATACACACTTCATTAATATATTTGGAAATCCTGAAAAAGCGTATCACAATTGTGTAGCTGTAAGAGGAGCTTCAGTATATGTACTCGGACCAATGCATCTTATTGTTTCCCGACTTCTCCCGTGGAAAGAACGAATTCAGGTTTTACGGGGAGCAGGTGATTGGATGGGTGCCTTGAACATGGCAATGACAATTTATGATGGTCAAGCTCATGGTGTTGTTGACCTTCCTAGAACTCTGGTTGCTGTACAGGAGACCATAATGTCCTACCTGGTTGAGTTGCTATTGTCATATGTAGAGGAAGTATTTTCATATATCTCAGTAGCGTTCTGCAACCAAATTGGAAAAAGGGATCAAGCTGATGACGTAAACAGCAAAAGCAGTTCTATGCACTCTGAAATAAAAGAGCAATACACCCGTGTTGGTGGAGTTGCAGTTGAATTCTGTGTCCATATCAAAAGGACTGACATACTTTTTGATGAAATCTTCTCCAAGTTTGTAGCTGTTCAACAGAGAGACACATTTTTGGAGCTGTTGGAGCCTTATATATTGAAGGACATGCTTGGTTCTCTTCCACCTGAGATTATGCAAGCACTGGTAGAGCATTATAGCCGCACAGGGTGGTTACAGCGCGTTGAACAATGTGTCCTTCACATGGATATTTCATCCTTGGATTTCAATCAGGTTGTTAGGTTATGCAGGGAGCACGGGCTGTATAGTGCTCTGGTGTATCTCTTCAATAAAGGATTGGATGATTTCAGGTCACCCTTAGAGGAGCTGTTGGTGGTCTTGCGAAATAGTCAAAGAGAAGGTGCTACTGCCCTTGGTTACAGGATGCTGGTTTATTTAAAGTACTGCTTTTCAGGCCTTGCTTTTCCACCAGGACAGGGAACAATTCCCCCCTCACGCTTGCCATCTCTGAGAACAGAACTTCTGCAATTTTTGTTGGAGGGTTCTGATGCCCCTAATTCAAGATCAGTCTCAAGTGTATTGCCAGGAGGAGAATATATTAACCTGTATCTTCTGTTAGAGCTAGATACTGAAGCTACTTTGGACGTTCTGAGATGTGCTTTCGTAGAAGATGAAATCTCAAAATCTGACTTTTCTTCTCATGATTCAGATATGCAGGATGGGAACAATTTAATGGCTCAAAATAAAAATTCAATGGTTCAGAACACAGTAGATTCTCTTATTCGCATTATTAGTAAGGACAGTTCTCAAACAGATGGATCTCCTAGCAATGATGACACTGGATCAGTAGTAGTGTGGCCTTCAAAGAAAGAGATAGATCATCTATTTGAGTTTATTGCATACTATGTTGCATGTGGAAGAGCCACTGTTTCGAAAAGTGTATTGTCTCAGATTTTGGAGTATTTGACCTCAGATAATAACTTTCCACCATGTGTTTCCGGAGATAGCATAACTTCAAAAAGAAGAGAGAAGCAGGTTCTGGGACTTCTTGAGGTAGTGCCTGAGACCGATTGGGATTCATCTTATGTGTTACAGCTATGCGAGAAAGCGCGATTTTACCAGGTTTGTGGCCTAATTCATACTAGCAGACATCAGTATCTTGCTGCTTTGGATTGCTACATGAAAGATGTAGAAGAACCCATTCATGCCTTCTCCTTTATCAACAAAACATTATTACAGCTGACTGACAAGGAATGCGCTGCTTTTCGATCAGAAATTATTTCTCGAATTCCAGAGCTTTTTTACTTAAACAGAGAGGGCACATTCTTCTTGGTTATCGATCATTTTACTATTGAAGAAGGTTCTCATATCCTCTCTAAACTTCGTTCCCATCCAAAAAGTCTGTTCCTTTACTTAAAAACAGTGATTGAGGTCCACTTATCCGGCACGCTTGACTTCTCAAGTTTAAGAAAAGATGATCTTGTGAGGGTTAAGGATCAATCGAAAGCAGTTGAGGCGTATCTGGAAAGGATCTCCGATTTCCCAAAGCTTTTGCGTAGTAATCCTGTTAATGTGACTGATGATATGATTGAACTTTATCTGGAGCTGTTATGTCAGTACGAGCGCAATTCAGTTCTCAAGTTCTTAGAGACATTTGATAGCTATCGTGTGGAACACTGTTTACGCCTGTGCCAGAAATATGGGATAACAGATGCTGCTTCTTTCTTGTTGGAAAGGGTTGGTGATGTCGGCAGTGCTCTTTTACTTACACTTTCCACCCTCAGTGACAAATTTATGAAGCTTGATACTGCTGTGGCAAGTCTGGCGTCAAATAATTCTGCTCGTACTGAGCATTTCAGCAATGCTTTAAAATTGGAAGAGGTGAACGACATTAACAGCATATTGCATGCCTGTATTGGGCTGTGCCAAAGGAACACCCATCGCTTAAATCCTGATGAATCAGAGGCGCTCTGGTTTAGATTACTTGACTCGTTTTGTGAGCCTTTAACAGATTCATTTGATGCTGGAACGGTATCTAAAGGAGAAGATGTTAAAACGACGGTGGCCAAGTCATTGGACTCAGAAGAGGATGAGATGGCTTTTATAATCAAGTGGAGAATCTCAAAGCTCCATAAAGGCTTTCATATATTAAGAAAGCTGTTCTCTCGGTTCATCAAGGAGATTGTTGAGGGCATGATAGGATATGTTCGCCTTCCAACTATCATGTCCAAACTCCTCTCTGATAATGGCAACCAAGAATTTGGTGATTTTAAATTCACAATTTTGGGGATGCTTAGCACATATGGCTTTGAAAGAAGAATTCTGGATACTGCCAAGTCCCTGATAGAAGATGATACATTCTACACTATGAGCATACTCAAGAAAGGGGCCTCCCATGGGTACGCCCCTCGGAGTCAAATATGTTGTTTATGTGATTGTCTTCTTGACAAGAACTCTTCTAGCTACATTCGCATTTTCAATTGCGGTCATGCAACGCATCTTCAATGTGAAGCTTTAGAGAATGGCGCATCAAGTAGTAGCTCATCCTCTGGATGCCCTGTTTGTATGCCTAAGAAGAAGTCTCAGAGGTCTAGAAGCAAATCGGTCCTTCCAGAGAAGAGTTTGGTGAAGGAGTTTTTGTCAAGAACCCAACAAACATACGGAACAACTTCCCACACACACGAAAGCAGTGCATCAGAGAATACGTACGGGCTTCAGCAAATATCAAGGTTTGATATCTTGACCAATTTGCAAAGAGACCGAGGATTAGTCGAGATTGAAAACATGCCTCAACTAAAGCTCGCACCTCCGGCTGTGTATCATGAGAAGGTTCAGAAAGGAACTGTTCTTTCACCGGCAGAAAGCAGCACTGATCTTTCAAGAATAGGGCAACAAAGTAAAACCAAGCAACTCAGGGACCTAAAAGTAAGGGGGTCGTCTCTCCGGTTACCCTTAAAATCAAACATATTTGGTAATGGCAAGGAGAAGACCAGCAAGCGATGA

Protein Analysis

1931

Amino Acids

213.31

Weight (kDa)

5.32

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_VPS8 PF23410 421 - 602 5.1e-23 Vps8 beta-propeller
VPS8_N PF23306 422 - 597 1.5e-06 VPS8-like, N-terminal beta propeller repeats
Beta-prop_WDR36-Utp21_1st PF25171 446 - 541 3.4e-06 WDR36/Utp21 first beta-propeller
Beta-prop_EML PF23409 470 - 546 1.5e-06 Echinoderm microtubule-associated protein first beta-propeller
TPR_Vps8 PF12816 968 - 1151 5.4e-51 Vps8 central TPR
TPR_Vps41 PF23556 1354 - 1526 3.5e-07 Vps41 TPR-like region
TPR_VPS8_2 PF25066 1573 - 1721 7.1e-06 VPS8-like, TPR-like repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4895
AarI CACCTGC 2 cut(s) 2101, 2525
AasI GACNNNNNNGTC 1 cut(s) 2641
AbsI CCTCGAGG 1 cut(s) 436
Acc16I TGCGCA 1 cut(s) 1109
Acc36I ACCTGC 4 cut(s) 1886, 2101, 2525, 3814
AccB1I GGYRCC 2 cut(s) 1178, 2549
AccBSI CCGCTC 1 cut(s) 495
AccII CGCG 3 cut(s) 464, 3012, 3901
AccIII TCCGGA 2 cut(s) 586, 3788
AcoI YGGCCR 2 cut(s) 342, 4854
AcuI CTGAAG 5 cut(s) 1149, 2139, 2436, 3441, 5489
AcyI GRCGYC 2 cut(s) 240, 4616
AdeI CACNNNGTG 2 cut(s) 3000, 3782
AfiI CCNNNNNNNGG 5 cut(s) 1269, 3034, 3266, 4707, 5187
AflIII ACRYGT 1 cut(s) 1637
AgeI ACCGGT 1 cut(s) 1658
AjiI CACGTC 2 cut(s) 1546, 1640
AjnI CCWGG 5 cut(s) 414, 2649, 3259, 3373, 3909
AleI CACNNNNGTG 1 cut(s) 2430
AloI GAACNNNNNNTCC 2 cut(s) 3009, 3041
Alw21I GWGCWC 4 cut(s) 488, 3087, 3103, 4549
AlwNI CAGNNNCTG 3 cut(s) 2266, 3829, 4505
Ama87I CYCGRG 2 cut(s) 404, 436
Aor13HI TCCGGA 2 cut(s) 586, 3788
AoxI GGCC 8 cut(s) 342, 411, 432, 3244, 3647, 3919, 4854, 5178
ArsI GACNNNNNNTTYG 2 cut(s) 2837, 2869
AseI ATTAAT 1 cut(s) 2394
AsiGI ACCGGT 1 cut(s) 1658
Asp700I GAANNNNTTC 4 cut(s) 1907, 2514, 3990, 5458
AspLEI GCGC 7 cut(s) 1110, 3012, 3901, 4049, 4416, 4759, 5318
AsuC2I CCSGG 4 cut(s) 322, 346, 405, 406
AsuII TTCGAA 3 cut(s) 24, 890, 3722
AvaI CYCGRG 2 cut(s) 404, 436
AvaII GGWCC 9 cut(s) 250, 283, 294, 584, 1272, 2468, 4222, 5395, 5707
BaeGI GKGCMC 2 cut(s) 1766, 4112
BaeI ACNNNNGTAYC 4 cut(s) 3929, 3962, 4587, 4620
BalI TGGCCA 1 cut(s) 4856
BamHI GGATCC 1 cut(s) 337
BanI GGYRCC 2 cut(s) 1178, 2549
BauI CACGAG 1 cut(s) 1043
BbsI GAAGAC 4 cut(s) 99, 1815, 5222, 5783
Bbv12I GWGCWC 4 cut(s) 488, 3087, 3103, 4549
BceAI ACGGC 3 cut(s) 227, 346, 1605
BcgI CGANNNNNNTGC 8 cut(s) 427, 456, 461, 490, 674, 708, 5462, 5496
BciT130I CCWGG 5 cut(s) 416, 2651, 3261, 3375, 3911
BciVI GTATCC 2 cut(s) 1229, 5110
BclI TGATCA 3 cut(s) 700, 1300, 2257
BcnI CCSGG 4 cut(s) 322, 346, 405, 406
BfmI CTRYAG 1 cut(s) 420
BfoI RGCGCY 1 cut(s) 4760
BfuAI ACCTGC 4 cut(s) 1886, 2101, 2525, 3814
BfuI GTATCC 2 cut(s) 1229, 5110
BlpI GCTNAGC 1 cut(s) 5084
BmcAI AGTACT 2 cut(s) 1391, 3233
Bme1390I CCNGG 9 cut(s) 322, 346, 405, 406, 416, 2651, 3261, 3375, 3911
Bme18I GGWCC 9 cut(s) 250, 283, 294, 584, 1272, 2468, 4222, 5395, 5707
BmeT110I CYCGRG 2 cut(s) 404, 436
BmgBI CACGTC 2 cut(s) 1546, 1640
BmrFI CCNGG 9 cut(s) 322, 346, 405, 406, 416, 2651, 3261, 3375, 3911
BoxI GACNNNNGTC 1 cut(s) 292
BpiI GAAGAC 4 cut(s) 99, 1815, 5222, 5783
BpmI CTGGAG 3 cut(s) 1578, 1599, 4412
Bpu10I CCTNAGC 2 cut(s) 110, 596
Bpu1102I GCTNAGC 1 cut(s) 5084
Bpu14I TTCGAA 3 cut(s) 24, 890, 3722
BpuEI CTTGAG 7 cut(s) 693, 1223, 3346, 3860, 4234, 4412, 5153
BpuMI CCSGG 4 cut(s) 322, 346, 405, 406
Bsa29I ATCGAT 1 cut(s) 4128
BsaAI YACGTR 1 cut(s) 5496
BsaBI GATNNNNATC 2 cut(s) 3057, 4452
BsaHI GRCGYC 2 cut(s) 240, 4616
BsaI GGTCTC 4 cut(s) 2223, 2627, 3848, 5541
BsaWI WCCGGW 5 cut(s) 223, 586, 1658, 3788, 5733
Bsc4I CCNNNNNNNGG 5 cut(s) 1269, 3034, 3266, 4707, 5187
Bse118I RCCGGY 2 cut(s) 1658, 5644
Bse1I ACTGG 7 cut(s) 534, 568, 1582, 1595, 2290, 2979, 3634
Bse3DI GCAATG 5 cut(s) 1341, 2370, 2571, 3625, 4657
Bse8I GATNNNNATC 2 cut(s) 3057, 4452
BseAI TCCGGA 2 cut(s) 586, 3788
BseBI CCWGG 5 cut(s) 416, 2651, 3261, 3375, 3911
BseCI ATCGAT 1 cut(s) 4128
BseJI GATNNNNATC 2 cut(s) 3057, 4452
BseLI CCNNNNNNNGG 5 cut(s) 1269, 3034, 3266, 4707, 5187
BseMI GCAATG 5 cut(s) 1341, 2370, 2571, 3625, 4657
BseNI ACTGG 7 cut(s) 534, 568, 1582, 1595, 2290, 2979, 3634
BseRI GAGGAG 9 cut(s) 175, 178, 493, 496, 1064, 2460, 3167, 3393, 5015
BseSI GKGCMC 2 cut(s) 1766, 4112
BseX3I CGGCCG 1 cut(s) 342
BseYI CCCAGC 1 cut(s) 728
BsgI GTGCAG 2 cut(s) 1568, 2250
Bsh1236I CGCG 3 cut(s) 464, 3012, 3901
Bsh1285I CGRYCG 3 cut(s) 135, 290, 345
BshFI GGCC 8 cut(s) 344, 413, 434, 3246, 3649, 3921, 4856, 5180
BshNI GGYRCC 2 cut(s) 1178, 2549
BshTI ACCGGT 1 cut(s) 1658
BshVI ATCGAT 1 cut(s) 4128
BsiEI CGRYCG 3 cut(s) 135, 290, 345
BsiHKAI GWGCWC 4 cut(s) 488, 3087, 3103, 4549
BsiHKCI CYCGRG 2 cut(s) 404, 436
BsiWI CGTACG 1 cut(s) 5496
BslFI GGGAC 8 cut(s) 104, 160, 867, 1511, 1556, 3846, 5113, 5720
BslI CCNNNNNNNGG 5 cut(s) 1269, 3034, 3266, 4707, 5187
BsmBI CGTCTC 2 cut(s) 369, 5733
BsmFI GGGAC 8 cut(s) 104, 160, 867, 1511, 1556, 3846, 5113, 5720
BsmI GAATGC 3 cut(s) 400, 739, 4049
BsnI GGCC 8 cut(s) 344, 413, 434, 3246, 3649, 3921, 4856, 5180
Bso31I GGTCTC 4 cut(s) 2223, 2627, 3848, 5541
BsoBI CYCGRG 2 cut(s) 404, 436
Bsp119I TTCGAA 3 cut(s) 24, 890, 3722
Bsp1286I GDGCHC 6 cut(s) 488, 1766, 3087, 3103, 4112, 4549
Bsp13I TCCGGA 2 cut(s) 586, 3788
Bsp1407I TGTACA 4 cut(s) 303, 1600, 2069, 2626
Bsp1720I GCTNAGC 1 cut(s) 5084
Bsp19I CCATGG 3 cut(s) 39, 368, 5185
BspANI GGCC 8 cut(s) 344, 413, 434, 3246, 3649, 3921, 4856, 5180
BspDI ATCGAT 1 cut(s) 4128
BspEI TCCGGA 2 cut(s) 586, 3788
BspFNI CGCG 3 cut(s) 464, 3012, 3901
BspHI TCATGA 2 cut(s) 3490, 5611
BspMAI CTGCAG 1 cut(s) 424
BspMI ACCTGC 4 cut(s) 1886, 2101, 2525, 3814
BspQI GCTCTTC 2 cut(s) 1125, 3703
BspT104I TTCGAA 3 cut(s) 24, 890, 3722
BspT107I GGYRCC 2 cut(s) 1178, 2549
BspTNI GGTCTC 4 cut(s) 2223, 2627, 3848, 5541
BsrBI CCGCTC 1 cut(s) 495
BsrDI GCAATG 5 cut(s) 1341, 2370, 2571, 3625, 4657
BsrFI RCCGGY 2 cut(s) 1658, 5644
BsrGI TGTACA 4 cut(s) 303, 1600, 2069, 2626
BsrI ACTGG 7 cut(s) 534, 568, 1582, 1595, 2290, 2979, 3634
BssAI RCCGGY 2 cut(s) 1658, 5644
BssNI GRCGYC 2 cut(s) 240, 4616
BssSI CACGAG 1 cut(s) 1043
BssT1I CCWWGG 5 cut(s) 39, 368, 3048, 3202, 5185
Bst2BI CACGAG 1 cut(s) 1043
Bst2UI CCWGG 5 cut(s) 416, 2651, 3261, 3375, 3911
BstACI GRCGYC 2 cut(s) 240, 4616
BstAPI GCANNNNNTGC 1 cut(s) 1562
BstAUI TGTACA 4 cut(s) 303, 1600, 2069, 2626
BstBAI YACGTR 1 cut(s) 5496
BstBI TTCGAA 3 cut(s) 24, 890, 3722
BstDSI CCRYGG 5 cut(s) 39, 368, 462, 2501, 5185
BstEII GGTNACC 2 cut(s) 3141, 5736
BstFNI CGCG 3 cut(s) 464, 3012, 3901
BstH2I RGCGCY 1 cut(s) 4760
BstHHI GCGC 7 cut(s) 1110, 3012, 3901, 4049, 4416, 4759, 5318
BstMCI CGRYCG 3 cut(s) 135, 290, 345
BstNI CCWGG 5 cut(s) 416, 2651, 3261, 3375, 3911
BstNSI RCATGY 4 cut(s) 2941, 3705, 4700, 5578
BstPAI GACNNNNGTC 1 cut(s) 292
BstPI GGTNACC 2 cut(s) 3141, 5736
BstSCI CCNGG 9 cut(s) 320, 344, 403, 404, 414, 2649, 3259, 3373, 3909
BstSFI CTRYAG 1 cut(s) 420
BstSLI GKGCMC 2 cut(s) 1766, 4112
BstSNI TACGTA 1 cut(s) 5496
BstUI CGCG 3 cut(s) 464, 3012, 3901
BstV2I GAAGAC 4 cut(s) 99, 1815, 5222, 5783
BstX2I RGATCY 4 cut(s) 337, 1919, 3608, 4320
BstXI CCANNNNNNTGG 5 cut(s) 2242, 3917, 4490, 4864, 5051
BstYI RGATCY 4 cut(s) 337, 1919, 3608, 4320
BstZI CGGCCG 1 cut(s) 342
Bsu15I ATCGAT 1 cut(s) 4128
BsuI GTATCC 2 cut(s) 1229, 5110
BsuRI GGCC 8 cut(s) 344, 413, 434, 3246, 3649, 3921, 4856, 5180
BsuTUI ATCGAT 1 cut(s) 4128
BtgI CCRYGG 5 cut(s) 39, 368, 462, 2501, 5185
BtgZI GCGATG 1 cut(s) 4715
BtrI CACGTC 2 cut(s) 1546, 1640
BtsI GCAGTG 2 cut(s) 4549, 5485
BveI ACCTGC 4 cut(s) 1886, 2101, 2525, 3814
CaiI CAGNNNCTG 3 cut(s) 2266, 3829, 4505
CciI TCATGA 2 cut(s) 3490, 5611
CfoI GCGC 7 cut(s) 1110, 3012, 3901, 4049, 4416, 4759, 5318
Cfr10I RCCGGY 2 cut(s) 1658, 5644
Cfr42I CCGCGG 1 cut(s) 465
Cfr9I CCCGGG 1 cut(s) 404
ClaI ATCGAT 1 cut(s) 4128
CpoI CGGWCCG 1 cut(s) 283
CseI GACGC 2 cut(s) 248, 4605
CsiI ACCWGGT 2 cut(s) 2649, 3909
CspAI ACCGGT 1 cut(s) 1658
CspI CGGWCCG 1 cut(s) 283
DraI TTTAAA 3 cut(s) 3228, 4659, 5062
DraIII CACNNNGTG 2 cut(s) 3000, 3782
DrdI GACNNNNNNGTC 1 cut(s) 2641
DseDI GACNNNNNNGTC 1 cut(s) 2641
EaeI YGGCCR 2 cut(s) 342, 4854
EagI CGGCCG 1 cut(s) 342
EciI GGCGGA 1 cut(s) 154
EclXI CGGCCG 1 cut(s) 342
Eco105I TACGTA 1 cut(s) 5496
Eco130I CCWWGG 5 cut(s) 39, 368, 3048, 3202, 5185
Eco147I AGGCCT 1 cut(s) 3246
Eco31I GGTCTC 4 cut(s) 2223, 2627, 3848, 5541
Eco32I GATATC 1 cut(s) 5527
Eco47I GGWCC 9 cut(s) 250, 283, 294, 584, 1272, 2468, 4222, 5395, 5707
Eco52I CGGCCG 1 cut(s) 342
Eco57I CTGAAG 5 cut(s) 1149, 2139, 2436, 3441, 5489
Eco88I CYCGRG 2 cut(s) 404, 436
Eco91I GGTNACC 2 cut(s) 3141, 5736
EcoO109I RGGNCCY 2 cut(s) 5178, 5707
EcoO65I GGTNACC 2 cut(s) 3141, 5736
EcoRI GAATTC 5 cut(s) 1799, 2514, 2819, 4077, 5109
EcoRII CCWGG 5 cut(s) 414, 2649, 3259, 3373, 3909
EcoRV GATATC 1 cut(s) 5527
EcoT14I CCWWGG 5 cut(s) 39, 368, 3048, 3202, 5185
EcoT22I ATGCAT 1 cut(s) 2478
ErhI CCWWGG 5 cut(s) 39, 368, 3048, 3202, 5185
Esp3I CGTCTC 2 cut(s) 369, 5733
FalI AAGNNNNNCTT 6 cut(s) 2434, 2466, 3221, 3253, 4254, 4286
FaqI GGGAC 8 cut(s) 104, 160, 867, 1511, 1556, 3846, 5113, 5720
FauI CCCGC 1 cut(s) 647
FauNDI CATATG 3 cut(s) 1605, 2671, 5092
FbaI TGATCA 3 cut(s) 700, 1300, 2257
FspI TGCGCA 1 cut(s) 1109
GlaI GCGC 7 cut(s) 1109, 3011, 3900, 4048, 4415, 4758, 5317
GsaI CCCAGC 1 cut(s) 732
GsuI CTGGAG 3 cut(s) 1578, 1599, 4412
HaeII RGCGCY 1 cut(s) 4760
HaeIII GGCC 8 cut(s) 344, 413, 434, 3246, 3649, 3921, 4856, 5180
HgaI GACGC 2 cut(s) 248, 4605
HhaI GCGC 7 cut(s) 1110, 3012, 3901, 4049, 4416, 4759, 5318
Hin1I GRCGYC 2 cut(s) 240, 4616
Hin6I GCGC 7 cut(s) 1108, 3010, 3899, 4047, 4414, 4757, 5316
HinP1I GCGC 7 cut(s) 1108, 3010, 3899, 4047, 4414, 4757, 5316
HincII GTYRAC 1 cut(s) 2602
HindII GTYRAC 1 cut(s) 2602
HindIII AAGCTT 4 cut(s) 1149, 4337, 4589, 5301
Hpy166II GTNNAC 8 cut(s) 851, 1330, 2090, 2602, 2749, 4225, 4472, 4675
Hpy8I GTNNAC 8 cut(s) 851, 1330, 2090, 2602, 2749, 4225, 4472, 4675
Hpy99I CGWCG 8 cut(s) 92, 101, 104, 107, 140, 242, 260, 4852
HpyCH4IV ACGT 5 cut(s) 1545, 1639, 2745, 3435, 5495
HpySE526I ACGT 5 cut(s) 1545, 1639, 2745, 3435, 5495
Hsp92I GRCGYC 2 cut(s) 240, 4616
HspAI GCGC 7 cut(s) 1108, 3010, 3899, 4047, 4414, 4757, 5316
Kpn2I TCCGGA 2 cut(s) 586, 3788
Ksp22I TGATCA 3 cut(s) 700, 1300, 2257
KspI CCGCGG 1 cut(s) 465
LguI GCTCTTC 2 cut(s) 1125, 3703
MabI ACCWGGT 2 cut(s) 2649, 3909
MaeII ACGT 5 cut(s) 1545, 1639, 2745, 3435, 5495
MbiI CCGCTC 1 cut(s) 495
MfeI CAATTG 3 cut(s) 579, 2428, 5269
MflI RGATCY 4 cut(s) 337, 1919, 3608, 4320
MhlI GDGCHC 6 cut(s) 488, 1766, 3087, 3103, 4112, 4549
MlsI TGGCCA 1 cut(s) 4856
MluNI TGGCCA 1 cut(s) 4856
MlyI GAGTC 5 cut(s) 50, 974, 4772, 4862, 5212
MmeI TCCRAC 7 cut(s) 111, 310, 1880, 2895, 3306, 4500, 5030
Mox20I TGGCCA 1 cut(s) 4856
Mph1103I ATGCAT 1 cut(s) 2478
MroI TCCGGA 2 cut(s) 586, 3788
MroXI GAANNNNTTC 4 cut(s) 1907, 2514, 3990, 5458
MscI TGGCCA 1 cut(s) 4856
MslI CAYNNNNRTG 3 cut(s) 2240, 2430, 2564
Msp20I TGGCCA 1 cut(s) 4856
MspA1I CMGCKG 4 cut(s) 425, 464, 1184, 4030
MspR9I CCNGG 9 cut(s) 322, 346, 405, 406, 416, 2651, 3261, 3375, 3911
MunI CAATTG 3 cut(s) 579, 2428, 5269
Mva1269I GAATGC 3 cut(s) 400, 739, 4049
MvaI CCWGG 5 cut(s) 416, 2651, 3261, 3375, 3911
MvnI CGCG 3 cut(s) 464, 3012, 3901
NciI CCSGG 4 cut(s) 322, 346, 405, 406
NcoI CCATGG 3 cut(s) 39, 368, 5185
NdeI CATATG 3 cut(s) 1605, 2671, 5092
NmuCI GTSAC 7 cut(s) 1526, 1954, 2079, 2281, 3141, 4363, 4574
NsbI TGCGCA 1 cut(s) 1109
NsiI ATGCAT 1 cut(s) 2478
NspI RCATGY 4 cut(s) 2941, 3705, 4700, 5578
NspV TTCGAA 3 cut(s) 24, 890, 3722
OliI CACNNNNGTG 1 cut(s) 2430
PaeI GCATGC 1 cut(s) 4700
PaeR7I CTCGAG 1 cut(s) 436
PagI TCATGA 2 cut(s) 3490, 5611
PaqCI CACCTGC 2 cut(s) 2101, 2525
PceI AGGCCT 1 cut(s) 3246
PciSI GCTCTTC 2 cut(s) 1125, 3703
PcsI WCGNNNNNNNCGW 3 cut(s) 96, 102, 264
PctI GAATGC 3 cut(s) 400, 739, 4049
PdmI GAANNNNTTC 4 cut(s) 1907, 2514, 3990, 5458
PfeI GAWTC 9 cut(s) 158, 1625, 2323, 3494, 3563, 3866, 4748, 4802, 4908
Pfl23II CGTACG 1 cut(s) 5496
PinAI ACCGGT 1 cut(s) 1658
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 5 cut(s) 50, 973, 4772, 4862, 5211
PpsI GAGTC 5 cut(s) 50, 973, 4772, 4862, 5211
Ppu21I YACGTR 1 cut(s) 5496
PpuMI RGGWCCY 1 cut(s) 5707
PshAI GACNNNNGTC 1 cut(s) 292
PshBI ATTAAT 1 cut(s) 2394
PsiI TTATAA 1 cut(s) 4895
Psp5II RGGWCCY 1 cut(s) 5707
Psp6I CCWGG 5 cut(s) 414, 2649, 3259, 3373, 3909
PspEI GGTNACC 2 cut(s) 3141, 5736
PspFI CCCAGC 1 cut(s) 728
PspGI CCWGG 5 cut(s) 414, 2649, 3259, 3373, 3909
PspLI CGTACG 1 cut(s) 5496
PspPPI RGGWCCY 1 cut(s) 5707
PspXI VCTCGAGB 1 cut(s) 436
PsrI GAACNNNNNNTAC 4 cut(s) 3577, 3609, 4455, 4487
PstI CTGCAG 1 cut(s) 424
PstNI CAGNNNCTG 3 cut(s) 2266, 3829, 4505
PsuI RGATCY 4 cut(s) 337, 1919, 3608, 4320
PvuI CGATCG 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 4030
RseI CAYNNNNRTG 3 cut(s) 2240, 2430, 2564
Rsr2I CGGWCCG 1 cut(s) 283
RsrII CGGWCCG 1 cut(s) 283
SacII CCGCGG 1 cut(s) 465
SapI GCTCTTC 2 cut(s) 1125, 3703
ScaI AGTACT 2 cut(s) 1391, 3233
SchI GAGTC 5 cut(s) 50, 974, 4772, 4862, 5212
ScrFI CCNGG 9 cut(s) 322, 346, 405, 406, 416, 2651, 3261, 3375, 3911
SduI GDGCHC 6 cut(s) 488, 1766, 3087, 3103, 4112, 4549
SexAI ACCWGGT 2 cut(s) 2649, 3909
SfcI CTRYAG 1 cut(s) 420
Sfr274I CTCGAG 1 cut(s) 436
Sfr303I CCGCGG 1 cut(s) 465
SfuI TTCGAA 3 cut(s) 24, 890, 3722
SgrBI CCGCGG 1 cut(s) 465
SinI GGWCC 9 cut(s) 250, 283, 294, 584, 1272, 2468, 4222, 5395, 5707
SlaI CTCGAG 1 cut(s) 436
SmaI CCCGGG 1 cut(s) 406
SmiMI CAYNNNNRTG 3 cut(s) 2240, 2430, 2564
SmlI CTYRAG 8 cut(s) 436, 708, 1202, 3361, 3839, 4249, 4427, 5168
SmoI CTYRAG 8 cut(s) 436, 708, 1202, 3361, 3839, 4249, 4427, 5168
SnaBI TACGTA 1 cut(s) 5496
SphI GCATGC 1 cut(s) 4700
SseBI AGGCCT 1 cut(s) 3246
StuI AGGCCT 1 cut(s) 3246
StyD4I CCNGG 9 cut(s) 320, 344, 403, 404, 414, 2649, 3259, 3373, 3909
StyI CCWWGG 5 cut(s) 39, 368, 3048, 3202, 5185
TaiI ACGT 5 cut(s) 1548, 1642, 2748, 3438, 5498
TaqII GACCGA 5 cut(s) 238, 304, 3872, 5383, 5565
TatI WGTACW 8 cut(s) 303, 1389, 1600, 2054, 2069, 2461, 2626, 3231
TauI GCSGC 9 cut(s) 413, 428, 431, 434, 457, 464, 467, 1184, 2994
TfiI GAWTC 9 cut(s) 158, 1625, 2323, 3494, 3563, 3866, 4748, 4802, 4908
TseFI GTSAC 7 cut(s) 1526, 1954, 2079, 2281, 3141, 4363, 4574
Tsp45I GTSAC 7 cut(s) 1526, 1954, 2079, 2281, 3141, 4363, 4574
TspGWI ACGGA 3 cut(s) 106, 827, 5466
TspMI CCCGGG 1 cut(s) 404
VpaK11BI GGWCC 9 cut(s) 250, 283, 294, 584, 1272, 2468, 4222, 5395, 5707
VspI ATTAAT 1 cut(s) 2394
XbaI TCTAGA 1 cut(s) 5381
XceI RCATGY 4 cut(s) 2941, 3705, 4700, 5578
XhoI CTCGAG 1 cut(s) 436
XmaI CCCGGG 1 cut(s) 404
XmnI GAANNNNTTC 4 cut(s) 1907, 2514, 3990, 5458
ZrmI AGTACT 2 cut(s) 1391, 3233
Zsp2I ATGCAT 1 cut(s) 2478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.