pycom07g16800

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002522
Physical Location & Seq
Reverse (-)
19251632 .. 19253440
1809 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1545 bp
ATGAGTGAAGAATATGGAGAGGGAAACCTCATCACACAAGCAGAGGATTTTCTAAACGAAATCTTCAGCAATTGGAGTGACTCCATTAAAGCACTTGAAACCTGCGAAGAGGTCTTGCCTCATGCAGAAGAGCTTCATCTTGTTTCGAGATGCATCAATTCATTGGCAATGAAAGTGAGTACCGATCCAGGTTCATTTAGTTGGCCTGTGTCGGGAAAGGATGCCTTGCAGAGCTCAGAGGGTGCGGTGTTTTGGAATGGAATATGCACAACAGCAAAGCCACATCCAGTCAGTGAAGATTGGTGGTATGAAGATGTCTCATTTCTGAAGTTTCCATTTTTCAAGAGGCTGATTCGAGCGGTTGAATTAGGAGGCATGAACCAAGAGAGGAAGGCTGGCTCCATAATGCACTATGCAAAGAGGCACCTTTCCTTGTTGGGTAGGCAATCGAGTTTCCATAATGGAAATCGTGCTGCCCCGTTATCAACTGTTCCTGCTGCATCTGATGGAGAGCAAAGGACTCTCCTTGAGGAGATTGTGGGATTACTTTCTGATCAGAAGGGCGTTGTGCCAACGAGCTTCCTGCTTAGGCTTCTCCGAACATCTATGATTTTGCAAGCTAGTGCATCATGTTGTGAGAGCTTAGAGAAACGAGTTGGGGCTCAATTGGATCGAGCAGTTCTTGAAGATCTTCTGATACCAAACATGGGGTACTCAGTGGAGACCCTATATGATATTGACTGCGTTCAGCGGATTCTTGACCACTTCATTCTCATGGACCGTGAATCAATTGATTATACTTCTAGCTGTATTGTTGATGAGGGCGTTTCTCATTCGCTGACTGCAATAATCTCGGTGGCTAACCTGGTTGATGGATATCTGGCTGAAGTTGCACCTGATGTTAACTTGAAACTGCAGAAATTTCAGTCGCTAGCTGCTGTTATCCCGGAATATGCCAGACCATTAGATGATGGAATTTATCGCGCCATTGATATATACCTCAAGGCCCATCCTTGGCTGACAGACTCCGAGAGGGAACATATCTGCCGGCTCATGAACTGCCAGAAGCTCTCATTGGAAGCTAGCACGCACGCAGCGCAGAATGAGAGGCTACCACTTCGCGTCATCGTCCAAGTTCTATTCTTCGAACAGCTTCGCCTGCGCACATCAATTTCCGGTTGGTTCTTTGTATCTGAGAACCTCGAGAACTCACAAAACCAAAGTAGAAATCTTGCGCTTGCCCGAAATGAGGCACCTGCTCAAGCTGGCACTGTGCGAGATCATGTTGTAACAGTTGATGACATGAGGGAACGAGTTTTGGAGCTTGAGAAAGAGTGCTTGAGCATGAAGCAAGAGATTGAGAAGCTGGTGAAGACAAAGGGGAGTTGGAACACATTCTTGAAGAGGTTTGGTTTGAGGCTCAAGTTAAAATCTGATGCAAAAAAAGCACCCAAACCCTGCAGTAATTCCAAAGAACAACCAACATCTATAGGTCCACTTGTTAATGGAAAACAAAATTATGAAAATAATAAATTAAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

515

Amino Acids

57.81

Weight (kDa)

5.68

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 100 - 362 1.9e-93 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1264
Acc16I TGCGCA 1 cut(s) 1163
Acc36I ACCTGC 2 cut(s) 110, 1264
AccB1I GGYRCC 2 cut(s) 423, 1252
AccBSI CCGCTC 1 cut(s) 359
AccII CGCG 2 cut(s) 984, 1122
AciI CCGC 3 cut(s) 245, 359, 751
AclWI GGATC 2 cut(s) 179, 678
AcsI RAATTY 2 cut(s) 920, 975
AcuI CTGAAG 3 cut(s) 49, 347, 906
AfaI GTAC 2 cut(s) 181, 713
AfiI CCNNNNNNNGG 4 cut(s) 212, 707, 1014, 1249
AgsI TTSAA 6 cut(s) 98, 343, 365, 686, 910, 1402
AjnI CCWGG 2 cut(s) 187, 864
AjuI GAANNNNNNNTTGG 2 cut(s) 1058, 1090
Alw21I GWGCWC 1 cut(s) 236
Alw26I GTCTC 2 cut(s) 322, 716
AlwI GGATC 2 cut(s) 179, 678
Ama87I CYCGRG 1 cut(s) 1202
AoxI GGCC 2 cut(s) 203, 1005
ApeKI GCWGC 4 cut(s) 473, 497, 935, 1094
ApoI RAATTY 2 cut(s) 920, 975
Asp700I GAANNNNTTC 4 cut(s) 132, 690, 1152, 1394
AspLEI GCGC 4 cut(s) 986, 1099, 1164, 1237
AspS9I GGNCC 3 cut(s) 778, 1006, 1493
AsuC2I CCSGG 1 cut(s) 947
AsuHPI GGTGA 1 cut(s) 1381
AsuII TTCGAA 1 cut(s) 1146
AsuNHI GCTAGC 2 cut(s) 931, 1082
AvaI CYCGRG 1 cut(s) 1202
AvaII GGWCC 2 cut(s) 778, 1493
BanI GGYRCC 2 cut(s) 423, 1252
BanII GRGCYC 2 cut(s) 236, 664
BbsI GAAGAC 1 cut(s) 1379
Bbv12I GWGCWC 1 cut(s) 236
BbvI GCAGC 4 cut(s) 460, 484, 922, 1106
BccI CCATC 4 cut(s) 500, 866, 965, 1017
BcgI CGANNNNNNTGC 4 cut(s) 565, 599, 834, 868
BciT130I CCWGG 2 cut(s) 189, 866
BclI TGATCA 1 cut(s) 553
BcnI CCSGG 1 cut(s) 947
BcoDI GTCTC 2 cut(s) 322, 716
BfaI CTAG 4 cut(s) 621, 804, 932, 1083
BfmI CTRYAG 3 cut(s) 914, 1459, 1488
BfuAI ACCTGC 2 cut(s) 110, 1264
BglII AGATCT 1 cut(s) 688
BisI GCNGC 4 cut(s) 474, 498, 936, 1095
BlsI GCNGC 4 cut(s) 475, 499, 937, 1096
Bme1390I CCNGG 3 cut(s) 189, 866, 947
Bme18I GGWCC 2 cut(s) 778, 1493
BmeT110I CYCGRG 1 cut(s) 1202
BmgT120I GGNCC 3 cut(s) 778, 1006, 1493
BmiI GGNNCC 3 cut(s) 400, 425, 1254
BmrFI CCNGG 3 cut(s) 189, 866, 947
BmsI GCATC 6 cut(s) 140, 162, 211, 509, 635, 1426
BmtI GCTAGC 2 cut(s) 935, 1086
BpiI GAAGAC 1 cut(s) 1379
Bpu10I CCTNAGC 1 cut(s) 587
Bpu14I TTCGAA 1 cut(s) 1146
BpuEI CTTGAG 6 cut(s) 548, 986, 1245, 1346, 1360, 1406
BpuMI CCSGG 1 cut(s) 947
BsaBI GATNNNNATC 1 cut(s) 876
BsaI GGTCTC 1 cut(s) 716
BsaJI CCNNGG 1 cut(s) 1013
BsaWI WCCGGW 1 cut(s) 1175
Bsc4I CCNNNNNNNGG 4 cut(s) 212, 707, 1014, 1249
Bse118I RCCGGY 1 cut(s) 1047
Bse1I ACTGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 174
Bse8I GATNNNNATC 1 cut(s) 876
BseBI CCWGG 2 cut(s) 189, 866
BseDI CCNNGG 1 cut(s) 1013
BseGI GGATG 3 cut(s) 226, 283, 1009
BseJI GATNNNNATC 1 cut(s) 876
BseLI CCNNNNNNNGG 4 cut(s) 212, 707, 1014, 1249
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 3 cut(s) 249, 729, 1185
BseNI ACTGG 1 cut(s) 287
BseRI GAGGAG 1 cut(s) 545
BseXI GCAGC 4 cut(s) 460, 484, 922, 1106
Bsh1236I CGCG 2 cut(s) 984, 1122
BshFI GGCC 2 cut(s) 205, 1007
BshNI GGYRCC 2 cut(s) 423, 1252
BsiHKAI GWGCWC 1 cut(s) 236
BsiHKCI CYCGRG 1 cut(s) 1202
BsiSI CCGG 3 cut(s) 947, 1048, 1176
BslI CCNNNNNNNGG 4 cut(s) 212, 707, 1014, 1249
BsmAI GTCTC 2 cut(s) 322, 716
BsnI GGCC 2 cut(s) 205, 1007
Bso31I GGTCTC 1 cut(s) 716
BsoBI CYCGRG 1 cut(s) 1202
Bsp119I TTCGAA 1 cut(s) 1146
Bsp1286I GDGCHC 2 cut(s) 236, 664
Bsp143I GATC 5 cut(s) 184, 553, 670, 688, 1279
BspACI CCGC 3 cut(s) 245, 359, 751
BspANI GGCC 2 cut(s) 205, 1007
BspCNI CTCAG 3 cut(s) 248, 728, 1186
BspFNI CGCG 2 cut(s) 984, 1122
BspHI TCATGA 1 cut(s) 1053
BspLI GGNNCC 3 cut(s) 400, 425, 1254
BspMAI CTGCAG 2 cut(s) 918, 1463
BspMI ACCTGC 2 cut(s) 110, 1264
BspOI GCTAGC 2 cut(s) 935, 1086
BspPI GGATC 2 cut(s) 179, 678
BspQI GCTCTTC 1 cut(s) 123
BspT104I TTCGAA 1 cut(s) 1146
BspT107I GGYRCC 2 cut(s) 423, 1252
BspTNI GGTCTC 1 cut(s) 716
BsrBI CCGCTC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 1047
BsrI ACTGG 1 cut(s) 287
BssAI RCCGGY 1 cut(s) 1047
BssECI CCNNGG 1 cut(s) 1013
BssMI GATC 5 cut(s) 184, 553, 670, 688, 1279
BssT1I CCWWGG 1 cut(s) 1013
Bst2UI CCWGG 2 cut(s) 189, 866
Bst4CI ACNGT 4 cut(s) 490, 782, 1273, 1294
Bst6I CTCTTC 3 cut(s) 102, 123, 1397
BstBI TTCGAA 1 cut(s) 1146
BstDEI CTNAG 5 cut(s) 235, 587, 643, 715, 1194
BstF5I GGATG 3 cut(s) 226, 283, 1009
BstFNI CGCG 2 cut(s) 984, 1122
BstHHI GCGC 4 cut(s) 986, 1099, 1164, 1237
BstKTI GATC 5 cut(s) 187, 556, 673, 691, 1282
BstMAI GTCTC 2 cut(s) 322, 716
BstMBI GATC 5 cut(s) 184, 553, 670, 688, 1279
BstMWI GCNNNNNNNGC 4 cut(s) 890, 1096, 1159, 1445
BstNI CCWGG 2 cut(s) 189, 866
BstSCI CCNGG 3 cut(s) 187, 864, 945
BstSFI CTRYAG 3 cut(s) 914, 1459, 1488
BstUI CGCG 2 cut(s) 984, 1122
BstV1I GCAGC 4 cut(s) 460, 484, 922, 1106
BstV2I GAAGAC 1 cut(s) 1379
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
BsuRI GGCC 2 cut(s) 205, 1007
BtsCI GGATG 3 cut(s) 226, 283, 1009
BtsIMutI CAGTG 3 cut(s) 298, 723, 1269
BveI ACCTGC 2 cut(s) 110, 1264
CciI TCATGA 1 cut(s) 1053
CfoI GCGC 4 cut(s) 986, 1099, 1164, 1237
Cfr10I RCCGGY 1 cut(s) 1047
Cfr13I GGNCC 3 cut(s) 778, 1006, 1493
CseI GACGC 1 cut(s) 1111
CsiI ACCWGGT 1 cut(s) 864
Csp6I GTAC 2 cut(s) 180, 712
CviAII CATG 9 cut(s) 122, 376, 630, 706, 775, 1054, 1283, 1303, 1345
CviQI GTAC 2 cut(s) 180, 712
DdeI CTNAG 5 cut(s) 235, 587, 643, 715, 1194
DpnI GATC 5 cut(s) 186, 555, 672, 690, 1281
DpnII GATC 5 cut(s) 184, 553, 670, 688, 1279
Eam1104I CTCTTC 3 cut(s) 102, 123, 1397
EarI CTCTTC 3 cut(s) 102, 123, 1397
Ecl136II GAGCTC 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 1013
Eco24I GRGCYC 2 cut(s) 236, 664
Eco31I GGTCTC 1 cut(s) 716
Eco32I GATATC 1 cut(s) 878
Eco47I GGWCC 2 cut(s) 778, 1493
Eco53kI GAGCTC 1 cut(s) 234
Eco57I CTGAAG 3 cut(s) 49, 347, 906
Eco88I CYCGRG 1 cut(s) 1202
EcoICRI GAGCTC 1 cut(s) 234
EcoRII CCWGG 2 cut(s) 187, 864
EcoRV GATATC 1 cut(s) 878
EcoT14I CCWWGG 1 cut(s) 1013
EcoT22I ATGCAT 1 cut(s) 155
EcoT38I GRGCYC 2 cut(s) 236, 664
ErhI CCWWGG 1 cut(s) 1013
FaeI CATG 9 cut(s) 125, 379, 633, 709, 778, 1057, 1286, 1306, 1348
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FatI CATG 9 cut(s) 121, 375, 629, 705, 774, 1053, 1282, 1302, 1344
FbaI TGATCA 1 cut(s) 553
Fnu4HI GCNGC 4 cut(s) 474, 498, 936, 1095
FokI GGATG 3 cut(s) 233, 270, 996
FriOI GRGCYC 2 cut(s) 236, 664
Fsp4HI GCNGC 4 cut(s) 474, 498, 936, 1095
FspBI CTAG 4 cut(s) 621, 804, 932, 1083
FspI TGCGCA 1 cut(s) 1163
GlaI GCGC 4 cut(s) 985, 1098, 1163, 1236
GluI GCNGC 4 cut(s) 474, 498, 936, 1095
HaeIII GGCC 2 cut(s) 205, 1007
HapII CCGG 3 cut(s) 947, 1048, 1176
HgaI GACGC 1 cut(s) 1111
HhaI GCGC 4 cut(s) 986, 1099, 1164, 1237
Hin1II CATG 9 cut(s) 125, 379, 633, 709, 778, 1057, 1286, 1306, 1348
Hin6I GCGC 4 cut(s) 984, 1097, 1162, 1235
HinP1I GCGC 4 cut(s) 984, 1097, 1162, 1235
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HinfI GANTC 6 cut(s) 80, 352, 520, 754, 785, 1025
HpaI GTTAAC 1 cut(s) 904
HpaII CCGG 3 cut(s) 947, 1048, 1176
HphI GGTGA 1 cut(s) 1381
Hpy166II GTNNAC 2 cut(s) 904, 1496
Hpy188III TCNNGA 8 cut(s) 147, 213, 343, 683, 758, 1054, 1204, 1399
Hpy8I GTNNAC 2 cut(s) 904, 1496
HpyAV CCTTC 2 cut(s) 385, 553
HpyCH4III ACNGT 4 cut(s) 490, 782, 1273, 1294
HpyF10VI GCNNNNNNNGC 4 cut(s) 890, 1096, 1159, 1445
HpyF3I CTNAG 5 cut(s) 235, 587, 643, 715, 1194
Hsp92II CATG 9 cut(s) 125, 379, 633, 709, 778, 1057, 1286, 1306, 1348
HspAI GCGC 4 cut(s) 984, 1097, 1162, 1235
KroI GCCGGC 1 cut(s) 1047
KroNI GCCGGC 1 cut(s) 1049
Ksp22I TGATCA 1 cut(s) 553
KspAI GTTAAC 1 cut(s) 904
Kzo9I GATC 5 cut(s) 184, 553, 670, 688, 1279
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 2 cut(s) 404, 1321
Lsp1109I GCAGC 4 cut(s) 460, 484, 922, 1106
LweI GCATC 6 cut(s) 140, 162, 211, 509, 635, 1426
MabI ACCWGGT 1 cut(s) 864
MaeI CTAG 4 cut(s) 621, 804, 932, 1083
MaeIII GTNAC 2 cut(s) 77, 1288
MalI GATC 5 cut(s) 186, 555, 672, 690, 1281
MbiI CCGCTC 1 cut(s) 359
MboI GATC 5 cut(s) 184, 553, 670, 688, 1279
MfeI CAATTG 3 cut(s) 70, 665, 789
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 2 cut(s) 236, 664
MlyI GAGTC 3 cut(s) 74, 514, 1019
MmeI TCCRAC 1 cut(s) 1367
Mph1103I ATGCAT 1 cut(s) 155
MroNI GCCGGC 1 cut(s) 1047
MroXI GAANNNNTTC 4 cut(s) 132, 690, 1152, 1394
MseI TTAA 5 cut(s) 87, 903, 1427, 1503, 1535
MslI CAYNNNNRTG 1 cut(s) 773
MspA1I CMGCKG 1 cut(s) 751
MspI CCGG 3 cut(s) 947, 1048, 1176
MspR9I CCNGG 3 cut(s) 189, 866, 947
MunI CAATTG 3 cut(s) 70, 665, 789
MvaI CCWGG 2 cut(s) 189, 866
MvnI CGCG 2 cut(s) 984, 1122
MwoI GCNNNNNNNGC 4 cut(s) 890, 1096, 1159, 1445
NaeI GCCGGC 1 cut(s) 1049
NciI CCSGG 1 cut(s) 947
NdeII GATC 5 cut(s) 184, 553, 670, 688, 1279
NgoMIV GCCGGC 1 cut(s) 1047
NheI GCTAGC 2 cut(s) 931, 1082
NlaIII CATG 9 cut(s) 125, 379, 633, 709, 778, 1057, 1286, 1306, 1348
NlaIV GGNNCC 3 cut(s) 400, 425, 1254
NmuCI GTSAC 1 cut(s) 77
NsbI TGCGCA 1 cut(s) 1163
NsiI ATGCAT 1 cut(s) 155
NspV TTCGAA 1 cut(s) 1146
PaeR7I CTCGAG 1 cut(s) 1202
PagI TCATGA 1 cut(s) 1053
PaqCI CACCTGC 1 cut(s) 1264
PciSI GCTCTTC 1 cut(s) 123
PdiI GCCGGC 1 cut(s) 1049
PdmI GAANNNNTTC 4 cut(s) 132, 690, 1152, 1394
PfeI GAWTC 3 cut(s) 352, 754, 785
PfoI TCCNGGA 1 cut(s) 945
PkrI GCNGC 4 cut(s) 475, 499, 937, 1096
PleI GAGTC 3 cut(s) 74, 514, 1019
PpsI GAGTC 3 cut(s) 74, 514, 1019
Psp124BI GAGCTC 1 cut(s) 236
Psp6I CCWGG 2 cut(s) 187, 864
PspGI CCWGG 2 cut(s) 187, 864
PspN4I GGNNCC 3 cut(s) 400, 425, 1254
PspPI GGNCC 3 cut(s) 778, 1006, 1493
PstI CTGCAG 2 cut(s) 918, 1463
PsuI RGATCY 1 cut(s) 688
RsaI GTAC 2 cut(s) 181, 713
RsaNI GTAC 2 cut(s) 180, 712
RseI CAYNNNNRTG 1 cut(s) 773
SacI GAGCTC 1 cut(s) 236
SapI GCTCTTC 1 cut(s) 123
SaqAI TTAA 5 cut(s) 87, 903, 1427, 1503, 1535
SatI GCNGC 4 cut(s) 474, 498, 936, 1095
Sau3AI GATC 5 cut(s) 184, 553, 670, 688, 1279
Sau96I GGNCC 3 cut(s) 778, 1006, 1493
SchI GAGTC 3 cut(s) 74, 514, 1019
ScrFI CCNGG 3 cut(s) 189, 866, 947
SduI GDGCHC 2 cut(s) 236, 664
SexAI ACCWGGT 1 cut(s) 864
SfaNI GCATC 6 cut(s) 140, 162, 211, 509, 635, 1426
SfcI CTRYAG 3 cut(s) 914, 1459, 1488
Sfr274I CTCGAG 1 cut(s) 1202
SfuI TTCGAA 1 cut(s) 1146
SinI GGWCC 2 cut(s) 778, 1493
SlaI CTCGAG 1 cut(s) 1202
SmiMI CAYNNNNRTG 1 cut(s) 773
SmlI CTYRAG 7 cut(s) 527, 1001, 1202, 1260, 1325, 1339, 1421
SmoI CTYRAG 7 cut(s) 527, 1001, 1202, 1260, 1325, 1339, 1421
SsiI CCGC 3 cut(s) 245, 359, 751
SspMI CTAG 4 cut(s) 621, 804, 932, 1083
SstI GAGCTC 1 cut(s) 236
StyD4I CCNGG 3 cut(s) 187, 864, 945
StyI CCWWGG 1 cut(s) 1013
TaaI ACNGT 4 cut(s) 490, 782, 1273, 1294
TaqI TCGA 6 cut(s) 146, 355, 449, 673, 1146, 1203
TfiI GAWTC 3 cut(s) 352, 754, 785
Tru1I TTAA 5 cut(s) 87, 903, 1427, 1503, 1535
Tru9I TTAA 5 cut(s) 87, 903, 1427, 1503, 1535
TscAI CASTG 3 cut(s) 298, 723, 1276
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 4 cut(s) 473, 497, 935, 1094
Tsp45I GTSAC 1 cut(s) 77
TspRI CASTG 3 cut(s) 298, 723, 1276
VpaK11BI GGWCC 2 cut(s) 778, 1493
XapI RAATTY 2 cut(s) 920, 975
XhoI CTCGAG 1 cut(s) 1202
XmnI GAANNNNTTC 4 cut(s) 132, 690, 1152, 1394
XspI CTAG 4 cut(s) 621, 804, 932, 1083
Zsp2I ATGCAT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.