pycom07g20100

AAA domain (Cdc48 subfamily)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007059
Physical Location & Seq
Forward (+)
22285864 .. 22288911
3048 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1860 bp
ATGCCTACGCCGGTTACTGTAGCCAGGCAATGCCTAACGCCGGAGGCAGCAAACGCTTTGGACGAGGCCGTGGCGGTCGCGCGGCGAAGAGGCCACGGCCAAACGACGTCACTGCACGCCATATCCGCTCTACTCTCCATCCCGTCCTCCGCGCTACGCGACGCCTGCGCGCGCGCCCGAAACTCCGCCTACGCGCCCCGACTGCAATTCAAGGCTCTGGAGCTCTGCCTCAGCGTCTCGCTCGACAGGGTCTCCTCGACCCAACTCGCCGACGACCCGCCCGTTTCGAACTCGCTCATGGCCGCCATCAAACGGTCCCAAGCCAACCAGCGGAGGCAGCCCGAAAATTACCACCTATACCACCAGCTCTCGCAGCAGTCCTCGATCTCCGCCATTAAAGTGGAGCTCCAGCACCTGATTCTGTCGATTCTCGACGACCCTGTTGTCAGTCGGGTCTTCGCTGAAGCGGGGTTTCGGAGCTCGGAAATAAAGCTTGCAATTTTACGCCCTTTTCCTCAGATTCTCAGATACCCGCGCTCCAGAGGCCACCATCCTCTGTTTCTCTGCAACTTAGCCGAGTACCCGGACCCCGGTCGCCCGACCCGAACCGTTTTAACTGATGGGGACGAGAATTCGAGGAGAATAGGAGAGGTTCTCGGGAGAAACAGAGTGCGGAATCCTCTGCTTGTGGGCATATTCGCTCACGACGCACTCAAGAGCTTCGTGGAGGCTCTGGAGAAAAGAGACGGCGGCGTTTTGCCGGCGGACCTGTCTGGGTTGTCTGTAATTTCAGTGGAAAACGATGTCTCAAAGTTTATCACCGCGGATAGCGATGCAGGGTCGGTGAATTTGAGGTTCGGGGAGGTGGGTCGGGTCGCAGAGCAGAGTTTGGGGCCTGGGATTGTTCTGAACATTGGGGACTTGAAGGCGTTTGTGGCTGAGAACGCGGTGGCTGACTCAGTGAGTCACGTGGTGACCGAGTTGACAAGGCTGCTGGAGCTTCAGCGCGGGAAAATTTGGTTGATTGGTGCAACGGCTAGCTATGGAAGTTACTTAAAGTTCGTGGAGAGGTTTCCCTCTGTGGAAAAAGACTGGGACTTGCAGCTTCTGCCCATCACTTCTCTCAGAGGTGCTTCCATGGCTGAATCATATCCCAGGTCCAGCTTGATGGAGTCGTTTGTTCCATTTGGTGGATTGTTTTCAGCACCTTCTGAGCTAAAGCTCCCAATCAGTTGCTCGTATCAATGGTTTCCCCGCAATCATCAGGGCAATGAAAAAAGTGAGCAAGAAGCGTATTCTGTTCCAATGGGAGGTATTACAGCTTCAGTGGCAGGTCAGCCCCCAGCTAGTTTGCCTTCTTGGTTGCAGATGGCTCCTCTTGGCACAAACAAGGGATTGGATATGAAGACCAAAGACGATGGTGTGTTATTGAGTGCCAAAGTTTCAGGATTGCAAAAGAAATGGGATGATAAATGCCAACATCTTCACGACTCTCGTCCCCTGCCCGAAGCAAACTTCTTTCCAACTATAGTAGGATTTCAGTCTCCTGAAGACAAAAGGTGCAATCACGACAACACCATCAATATATCTTCACGTAAAATTGAGTGCAAGAATGTGCAAACACAATCAAGCCTTCCTCCAAAGGCTAAGAATGACAGTTTCTCTTCCGAAGTCTGGGAAAAAACTTCAAAAGATGAAGATCTTGAGTCGCCTGGCCTCAGGTCACCCTGCTTGTCCAACTCTAGTGTGGTTGATGGAACATCTGCCACATCAACCACTTCCGTCACCACAGATTTAGGGTTAGGAATATGCTCTTCTCCTGCTAGTAATACACCGAATAAACCTCCAGATCGATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

620

Amino Acids

67.31

Weight (kDa)

7.2

Isoelectric Point (pI)

56.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NBD_SMAX1 PF23569 211 - 375 2.4e-20 SMAX1 nucleotide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 443
AatII GACGTC 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 1322
AccB7I CCANNNNNTGG 1 cut(s) 1190
AccBSI CCGCTC 1 cut(s) 128
AcoI YGGCCR 2 cut(s) 97, 300
AcsI RAATTY 3 cut(s) 631, 847, 1014
AcuI CTGAAG 4 cut(s) 483, 986, 1308, 1569
AcvI CACGTG 1 cut(s) 970
AcyI GRCGYC 2 cut(s) 107, 162
AdeI CACNNNGTG 1 cut(s) 973
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 7 cut(s) 40, 312, 330, 590, 1190, 1310, 1674
AgsI TTSAA 3 cut(s) 211, 925, 1689
AjnI CCWGG 4 cut(s) 23, 895, 1154, 1711
Alw21I GWGCWC 3 cut(s) 225, 408, 482
Alw26I GTCTC 5 cut(s) 241, 256, 738, 811, 1548
AlwNI CAGNNNCTG 2 cut(s) 415, 1108
Ama87I CYCGRG 1 cut(s) 656
AoxI GGCC 7 cut(s) 66, 91, 97, 300, 544, 893, 1714
ApeKI GCWGC 5 cut(s) 47, 337, 373, 991, 1102
ApoI RAATTY 3 cut(s) 631, 847, 1014
AspLEI GCGC 9 cut(s) 82, 154, 170, 172, 174, 176, 196, 537, 1008
AspS9I GGNCC 5 cut(s) 315, 586, 766, 893, 1158
AsuC2I CCSGG 2 cut(s) 584, 591
AsuHPI GGTGA 5 cut(s) 811, 856, 985, 1716, 1777
AsuII TTCGAA 1 cut(s) 287
AsuNHI GCTAGC 1 cut(s) 1037
AvaI CYCGRG 1 cut(s) 656
AvaII GGWCC 4 cut(s) 315, 586, 766, 1158
AxyI CCTNAGG 1 cut(s) 1718
BanII GRGCYC 3 cut(s) 225, 408, 482
BarI GAAGNNNNNNTAC 2 cut(s) 1306, 1338
BbrPI CACGTG 1 cut(s) 970
BbsI GAAGAC 3 cut(s) 448, 1412, 1557
Bbv12I GWGCWC 3 cut(s) 225, 408, 482
BbvCI CCTCAGC 1 cut(s) 230
BbvI GCAGC 5 cut(s) 59, 349, 385, 978, 1114
BceAI ACGGC 4 cut(s) 53, 112, 763, 1050
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 4 cut(s) 25, 897, 1156, 1713
BcnI CCSGG 2 cut(s) 584, 591
BcoDI GTCTC 5 cut(s) 241, 256, 738, 811, 1548
BfaI CTAG 4 cut(s) 1038, 1347, 1743, 1824
BfmI CTRYAG 2 cut(s) 18, 1527
BfuAI ACCTGC 1 cut(s) 1322
BglII AGATCT 1 cut(s) 1699
BisI GCNGC 8 cut(s) 48, 83, 303, 338, 374, 751, 992, 1103
BlsI GCNGC 8 cut(s) 49, 84, 304, 339, 375, 752, 993, 1104
Bme1390I CCNGG 6 cut(s) 25, 584, 591, 897, 1156, 1713
Bme18I GGWCC 4 cut(s) 315, 586, 766, 1158
BmeT110I CYCGRG 1 cut(s) 656
BmgT120I GGNCC 5 cut(s) 315, 586, 766, 893, 1158
BmiI GGNNCC 4 cut(s) 317, 588, 894, 1374
BmrFI CCNGG 6 cut(s) 25, 584, 591, 897, 1156, 1713
BmrI ACTGGG 1 cut(s) 1102
BmsI GCATC 1 cut(s) 823
BmtI GCTAGC 1 cut(s) 1041
BmuI ACTGGG 1 cut(s) 1102
BoxI GACNNNNGTC 2 cut(s) 591, 1494
BpiI GAAGAC 3 cut(s) 448, 1412, 1557
BplI GAGNNNNNCTC 2 cut(s) 639, 671
BpmI CTGGAG 6 cut(s) 239, 392, 523, 755, 1016, 1830
Bpu10I CCTNAGC 1 cut(s) 230
Bpu14I TTCGAA 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 698, 1724
BpuMI CCSGG 2 cut(s) 584, 591
Bsa29I ATCGAT 1 cut(s) 1852
BsaAI YACGTR 2 cut(s) 970, 1595
BsaBI GATNNNNATC 1 cut(s) 1698
BsaHI GRCGYC 2 cut(s) 107, 162
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 7 cut(s) 69, 94, 589, 822, 896, 1137, 1154
BsaXI ACNNNNNCTCC 4 cut(s) 236, 266, 521, 551
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 312, 330, 590, 1190, 1310, 1674
Bse118I RCCGGY 2 cut(s) 10, 760
Bse1I ACTGG 1 cut(s) 1097
Bse21I CCTNAGG 1 cut(s) 1718
Bse3DI GCAATG 2 cut(s) 35, 1276
Bse8I GATNNNNATC 1 cut(s) 1698
BseBI CCWGG 4 cut(s) 25, 897, 1156, 1713
BseCI ATCGAT 1 cut(s) 1852
BseDI CCNNGG 7 cut(s) 69, 94, 589, 822, 896, 1137, 1154
BseGI GGATG 3 cut(s) 138, 550, 1471
BseJI GATNNNNATC 1 cut(s) 1698
BseLI CCNNNNNNNGG 7 cut(s) 40, 312, 330, 590, 1190, 1310, 1674
BseMI GCAATG 2 cut(s) 35, 1276
BseMII CTCAG 8 cut(s) 244, 530, 538, 930, 972, 1138, 1203, 1732
BseNI ACTGG 1 cut(s) 1097
BsePI GCGCGC 3 cut(s) 168, 170, 172
BseRI GAGGAG 3 cut(s) 244, 652, 1365
BseXI GCAGC 5 cut(s) 59, 349, 385, 978, 1114
BseYI CCCAGC 1 cut(s) 1342
BsgI GTGCAG 1 cut(s) 98
Bsh1285I CGRYCG 3 cut(s) 78, 595, 1856
BshFI GGCC 7 cut(s) 68, 93, 99, 302, 546, 895, 1716
BshVI ATCGAT 1 cut(s) 1852
BsiEI CGRYCG 3 cut(s) 78, 595, 1856
BsiHKAI GWGCWC 3 cut(s) 225, 408, 482
BsiHKCI CYCGRG 1 cut(s) 656
BsiSI CCGG 5 cut(s) 11, 41, 584, 591, 761
BslFI GGGAC 5 cut(s) 301, 638, 932, 1109, 1482
BslI CCNNNNNNNGG 7 cut(s) 40, 312, 330, 590, 1190, 1310, 1674
BsmAI GTCTC 5 cut(s) 241, 256, 738, 811, 1548
BsmBI CGTCTC 2 cut(s) 241, 738
BsmFI GGGAC 5 cut(s) 301, 638, 932, 1109, 1482
BsnI GGCC 7 cut(s) 68, 93, 99, 302, 546, 895, 1716
Bso31I GGTCTC 1 cut(s) 256
BsoBI CYCGRG 1 cut(s) 656
Bsp119I TTCGAA 1 cut(s) 287
Bsp1286I GDGCHC 3 cut(s) 225, 408, 482
Bsp143I GATC 4 cut(s) 384, 1699, 1849, 1853
Bsp19I CCATGG 1 cut(s) 1137
BspANI GGCC 7 cut(s) 68, 93, 99, 302, 546, 895, 1716
BspCNI CTCAG 8 cut(s) 243, 529, 537, 931, 971, 1137, 1204, 1731
BspDI ATCGAT 1 cut(s) 1852
BspLI GGNNCC 4 cut(s) 317, 588, 894, 1374
BspMI ACCTGC 1 cut(s) 1322
BspOI GCTAGC 1 cut(s) 1041
BspQI GCTCTTC 1 cut(s) 1819
BspT104I TTCGAA 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 256
BsrBI CCGCTC 1 cut(s) 128
BsrDI GCAATG 2 cut(s) 35, 1276
BsrFI RCCGGY 2 cut(s) 10, 760
BsrI ACTGG 1 cut(s) 1097
BssAI RCCGGY 2 cut(s) 10, 760
BssECI CCNNGG 7 cut(s) 69, 94, 589, 822, 896, 1137, 1154
BssHII GCGCGC 3 cut(s) 168, 170, 172
BssMI GATC 4 cut(s) 384, 1699, 1849, 1853
BssNI GRCGYC 2 cut(s) 107, 162
BssT1I CCWWGG 1 cut(s) 1137
Bst2UI CCWGG 4 cut(s) 25, 897, 1156, 1713
Bst4CI ACNGT 4 cut(s) 19, 315, 610, 1658
Bst6I CTCTTC 3 cut(s) 82, 1669, 1819
BstACI GRCGYC 2 cut(s) 107, 162
BstAPI GCANNNNNTGC 1 cut(s) 1108
BstBAI YACGTR 2 cut(s) 970, 1595
BstBI TTCGAA 1 cut(s) 287
BstC8I GCNNGC 8 cut(s) 117, 166, 170, 172, 174, 495, 762, 1039
BstDSI CCRYGG 4 cut(s) 69, 94, 822, 1137
BstEII GGTNACC 2 cut(s) 973, 1722
BstF5I GGATG 3 cut(s) 138, 550, 1471
BstHHI GCGC 9 cut(s) 82, 154, 170, 172, 174, 176, 196, 537, 1008
BstKTI GATC 4 cut(s) 387, 1702, 1852, 1856
BstMAI GTCTC 5 cut(s) 241, 256, 738, 811, 1548
BstMBI GATC 4 cut(s) 384, 1699, 1849, 1853
BstMCI CGRYCG 3 cut(s) 78, 595, 1856
BstNI CCWGG 4 cut(s) 25, 897, 1156, 1713
BstPAI GACNNNNGTC 2 cut(s) 591, 1494
BstPI GGTNACC 2 cut(s) 973, 1722
BstSCI CCNGG 6 cut(s) 23, 582, 589, 895, 1154, 1711
BstSFI CTRYAG 2 cut(s) 18, 1527
BstV1I GCAGC 5 cut(s) 59, 349, 385, 978, 1114
BstV2I GAAGAC 3 cut(s) 448, 1412, 1557
BstX2I RGATCY 1 cut(s) 1699
BstXI CCANNNNNNTGG 2 cut(s) 400, 1168
BstYI RGATCY 1 cut(s) 1699
Bsu15I ATCGAT 1 cut(s) 1852
Bsu36I CCTNAGG 1 cut(s) 1718
BsuRI GGCC 7 cut(s) 68, 93, 99, 302, 546, 895, 1716
BsuTUI ATCGAT 1 cut(s) 1852
BtgI CCRYGG 4 cut(s) 69, 94, 822, 1137
BtgZI GCGATG 1 cut(s) 846
BtsCI GGATG 3 cut(s) 138, 550, 1471
BtsI GCAGTG 1 cut(s) 110
BtsIMutI CAGTG 4 cut(s) 110, 798, 966, 1332
BveI ACCTGC 1 cut(s) 1322
Cac8I GCNNGC 8 cut(s) 117, 166, 170, 172, 174, 495, 762, 1039
CaiI CAGNNNCTG 2 cut(s) 415, 1108
CfoI GCGC 9 cut(s) 82, 154, 170, 172, 174, 176, 196, 537, 1008
Cfr10I RCCGGY 2 cut(s) 10, 760
Cfr13I GGNCC 5 cut(s) 315, 586, 766, 893, 1158
Cfr42I CCGCGG 1 cut(s) 825
ClaI ATCGAT 1 cut(s) 1852
CseI GACGC 3 cut(s) 170, 223, 716
Csp6I GTAC 1 cut(s) 580
CviAII CATG 2 cut(s) 298, 1138
CviQI GTAC 1 cut(s) 580
DpnI GATC 4 cut(s) 386, 1701, 1851, 1855
DpnII GATC 4 cut(s) 384, 1699, 1849, 1853
DraIII CACNNNGTG 1 cut(s) 973
DrdI GACNNNNNNGTC 1 cut(s) 443
DseDI GACNNNNNNGTC 1 cut(s) 443
EaeI YGGCCR 2 cut(s) 97, 300
Eam1104I CTCTTC 3 cut(s) 82, 1669, 1819
EarI CTCTTC 3 cut(s) 82, 1669, 1819
EciI GGCGGA 3 cut(s) 175, 379, 779
Ecl136II GAGCTC 3 cut(s) 223, 406, 480
Eco130I CCWWGG 1 cut(s) 1137
Eco24I GRGCYC 3 cut(s) 225, 408, 482
Eco31I GGTCTC 1 cut(s) 256
Eco47I GGWCC 4 cut(s) 315, 586, 766, 1158
Eco53kI GAGCTC 3 cut(s) 223, 406, 480
Eco57I CTGAAG 4 cut(s) 483, 986, 1308, 1569
Eco72I CACGTG 1 cut(s) 970
Eco81I CCTNAGG 1 cut(s) 1718
Eco88I CYCGRG 1 cut(s) 656
Eco91I GGTNACC 2 cut(s) 973, 1722
EcoICRI GAGCTC 3 cut(s) 223, 406, 480
EcoO109I RGGNCCY 1 cut(s) 893
EcoO65I GGTNACC 2 cut(s) 973, 1722
EcoRI GAATTC 1 cut(s) 631
EcoRII CCWGG 4 cut(s) 23, 895, 1154, 1711
EcoT14I CCWWGG 1 cut(s) 1137
EcoT38I GRGCYC 3 cut(s) 225, 408, 482
ErhI CCWWGG 1 cut(s) 1137
Esp3I CGTCTC 2 cut(s) 241, 738
FaeI CATG 2 cut(s) 301, 1141
FaqI GGGAC 5 cut(s) 301, 638, 932, 1109, 1482
FatI CATG 2 cut(s) 297, 1137
FauI CCCGC 5 cut(s) 285, 460, 540, 1001, 1262
Fnu4HI GCNGC 8 cut(s) 48, 83, 303, 338, 374, 751, 992, 1103
FokI GGATG 3 cut(s) 125, 537, 1478
FriOI GRGCYC 3 cut(s) 225, 408, 482
Fsp4HI GCNGC 8 cut(s) 48, 83, 303, 338, 374, 751, 992, 1103
FspBI CTAG 4 cut(s) 1038, 1347, 1743, 1824
GlaI GCGC 9 cut(s) 81, 153, 169, 171, 173, 175, 195, 536, 1007
GluI GCNGC 8 cut(s) 48, 83, 303, 338, 374, 751, 992, 1103
GsaI CCCAGC 1 cut(s) 1346
GsuI CTGGAG 6 cut(s) 239, 392, 523, 755, 1016, 1830
HaeIII GGCC 7 cut(s) 68, 93, 99, 302, 546, 895, 1716
HapII CCGG 5 cut(s) 11, 41, 584, 591, 761
HgaI GACGC 3 cut(s) 170, 223, 716
HhaI GCGC 9 cut(s) 82, 154, 170, 172, 174, 176, 196, 537, 1008
Hin1I GRCGYC 2 cut(s) 107, 162
Hin1II CATG 2 cut(s) 301, 1141
Hin6I GCGC 9 cut(s) 80, 152, 168, 170, 172, 174, 194, 535, 1006
HinP1I GCGC 9 cut(s) 80, 152, 168, 170, 172, 174, 194, 535, 1006
HincII GTYRAC 1 cut(s) 984
HindII GTYRAC 1 cut(s) 984
HindIII AAGCTT 1 cut(s) 491
HpaII CCGG 5 cut(s) 11, 41, 584, 591, 761
HphI GGTGA 5 cut(s) 811, 856, 985, 1716, 1777
Hpy166II GTNNAC 1 cut(s) 984
Hpy188I TCNGA 8 cut(s) 477, 484, 519, 527, 909, 1127, 1213, 1669
Hpy8I GTNNAC 1 cut(s) 984
Hpy99I CGWCG 5 cut(s) 109, 164, 275, 437, 710
HpyAV CCTTC 4 cut(s) 919, 1218, 1365, 1643
HpyCH4III ACNGT 4 cut(s) 19, 315, 610, 1658
HpyCH4IV ACGT 3 cut(s) 107, 969, 1594
HpySE526I ACGT 3 cut(s) 107, 969, 1594
Hsp92I GRCGYC 2 cut(s) 107, 162
Hsp92II CATG 2 cut(s) 301, 1141
HspAI GCGC 9 cut(s) 80, 152, 168, 170, 172, 174, 194, 535, 1006
KroI GCCGGC 1 cut(s) 760
KroNI GCCGGC 1 cut(s) 762
KspI CCGCGG 1 cut(s) 825
Kzo9I GATC 4 cut(s) 384, 1699, 1849, 1853
LguI GCTCTTC 1 cut(s) 1819
LmnI GCTCC 8 cut(s) 220, 403, 411, 477, 542, 997, 1227, 1378
Lsp1109I GCAGC 5 cut(s) 59, 349, 385, 978, 1114
LweI GCATC 1 cut(s) 823
MaeI CTAG 4 cut(s) 1038, 1347, 1743, 1824
MaeII ACGT 3 cut(s) 107, 969, 1594
MaeIII GTNAC 7 cut(s) 13, 108, 965, 973, 1049, 1722, 1783
MalI GATC 4 cut(s) 386, 1701, 1851, 1855
MauBI CGCGCGCG 1 cut(s) 170
MbiI CCGCTC 1 cut(s) 128
MboI GATC 4 cut(s) 384, 1699, 1849, 1853
MboII GAAGA 9 cut(s) 99, 448, 1417, 1475, 1562, 1581, 1656, 1709, 1806
MflI RGATCY 1 cut(s) 1699
MhlI GDGCHC 3 cut(s) 225, 408, 482
MluCI AATT 8 cut(s) 206, 346, 498, 631, 786, 847, 1014, 1599
MlyI GAGTC 5 cut(s) 950, 973, 1181, 1484, 1715
MmeI TCCRAC 2 cut(s) 1547, 1761
MroNI GCCGGC 1 cut(s) 760
MseI TTAA 3 cut(s) 396, 614, 1055
MslI CAYNNNNRTG 1 cut(s) 398
MspA1I CMGCKG 2 cut(s) 331, 824
MspI CCGG 5 cut(s) 11, 41, 584, 591, 761
MspR9I CCNGG 6 cut(s) 25, 584, 591, 897, 1156, 1713
MvaI CCWGG 4 cut(s) 25, 897, 1156, 1713
NaeI GCCGGC 1 cut(s) 762
NciI CCSGG 2 cut(s) 584, 591
NcoI CCATGG 1 cut(s) 1137
NdeII GATC 4 cut(s) 384, 1699, 1849, 1853
NgoMIV GCCGGC 1 cut(s) 760
NheI GCTAGC 1 cut(s) 1037
NlaIII CATG 2 cut(s) 301, 1141
NlaIV GGNNCC 4 cut(s) 317, 588, 894, 1374
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 5 cut(s) 108, 965, 973, 1722, 1783
NspV TTCGAA 1 cut(s) 287
PauI GCGCGC 3 cut(s) 168, 170, 172
PciSI GCTCTTC 1 cut(s) 1819
PcsI WCGNNNNNNNCGW 4 cut(s) 60, 279, 601, 975
PdiI GCCGGC 1 cut(s) 762
PfeI GAWTC 5 cut(s) 418, 427, 520, 676, 1145
PflFI GACNNNGTC 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 1190
PkrI GCNGC 8 cut(s) 49, 84, 304, 339, 375, 752, 993, 1104
Ple19I CGATCG 1 cut(s) 1856
PleI GAGTC 5 cut(s) 950, 972, 1180, 1484, 1714
PmaCI CACGTG 1 cut(s) 970
PmlI CACGTG 1 cut(s) 970
PpsI GAGTC 5 cut(s) 950, 972, 1180, 1484, 1714
Ppu21I YACGTR 2 cut(s) 970, 1595
PshAI GACNNNNGTC 2 cut(s) 591, 1494
Psp124BI GAGCTC 3 cut(s) 225, 408, 482
Psp6I CCWGG 4 cut(s) 23, 895, 1154, 1711
PspCI CACGTG 1 cut(s) 970
PspEI GGTNACC 2 cut(s) 973, 1722
PspFI CCCAGC 1 cut(s) 1342
PspGI CCWGG 4 cut(s) 23, 895, 1154, 1711
PspN4I GGNNCC 4 cut(s) 317, 588, 894, 1374
PspPI GGNCC 5 cut(s) 315, 586, 766, 893, 1158
PstNI CAGNNNCTG 2 cut(s) 415, 1108
PsuI RGATCY 1 cut(s) 1699
PsyI GACNNNGTC 1 cut(s) 248
PteI GCGCGC 3 cut(s) 168, 170, 172
PvuI CGATCG 1 cut(s) 1856
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
RseI CAYNNNNRTG 1 cut(s) 398
SacI GAGCTC 3 cut(s) 225, 408, 482
SacII CCGCGG 1 cut(s) 825
SapI GCTCTTC 1 cut(s) 1819
SaqAI TTAA 3 cut(s) 396, 614, 1055
SatI GCNGC 8 cut(s) 48, 83, 303, 338, 374, 751, 992, 1103
Sau3AI GATC 4 cut(s) 384, 1699, 1849, 1853
Sau96I GGNCC 5 cut(s) 315, 586, 766, 893, 1158
SchI GAGTC 5 cut(s) 950, 973, 1181, 1484, 1715
ScrFI CCNGG 6 cut(s) 25, 584, 591, 897, 1156, 1713
SduI GDGCHC 3 cut(s) 225, 408, 482
SfaNI GCATC 1 cut(s) 823
SfcI CTRYAG 2 cut(s) 18, 1527
Sfr303I CCGCGG 1 cut(s) 825
SfuI TTCGAA 1 cut(s) 287
SgrBI CCGCGG 1 cut(s) 825
SinI GGWCC 4 cut(s) 315, 586, 766, 1158
SmiMI CAYNNNNRTG 1 cut(s) 398
SmlI CTYRAG 2 cut(s) 713, 1703
SmoI CTYRAG 2 cut(s) 713, 1703
Sse9I AATT 8 cut(s) 206, 346, 498, 631, 786, 847, 1014, 1599
SspMI CTAG 4 cut(s) 1038, 1347, 1743, 1824
SstI GAGCTC 3 cut(s) 225, 408, 482
StyD4I CCNGG 6 cut(s) 23, 582, 589, 895, 1154, 1711
StyI CCWWGG 1 cut(s) 1137
TaaI ACNGT 4 cut(s) 19, 315, 610, 1658
TaiI ACGT 3 cut(s) 110, 972, 1597
TaqI TCGA 8 cut(s) 243, 257, 287, 383, 425, 432, 635, 1852
TaqII GACCGA 1 cut(s) 992
TasI AATT 8 cut(s) 206, 346, 498, 631, 786, 847, 1014, 1599
TauI GCSGC 3 cut(s) 85, 305, 753
TfiI GAWTC 5 cut(s) 418, 427, 520, 676, 1145
Tru1I TTAA 3 cut(s) 396, 614, 1055
Tru9I TTAA 3 cut(s) 396, 614, 1055
TscAI CASTG 4 cut(s) 117, 798, 966, 1332
TseFI GTSAC 5 cut(s) 108, 965, 973, 1722, 1783
TseI GCWGC 5 cut(s) 47, 337, 373, 991, 1102
Tsp45I GTSAC 5 cut(s) 108, 965, 973, 1722, 1783
TspDTI ATGAA 3 cut(s) 1287, 1418, 1710
TspGWI ACGGA 1 cut(s) 1771
TspRI CASTG 4 cut(s) 117, 798, 966, 1332
Tth111I GACNNNGTC 1 cut(s) 248
Van91I CCANNNNNTGG 1 cut(s) 1190
VpaK11BI GGWCC 4 cut(s) 315, 586, 766, 1158
XapI RAATTY 3 cut(s) 631, 847, 1014
XcmI CCANNNNNNNNNTGG 1 cut(s) 1744
XspI CTAG 4 cut(s) 1038, 1347, 1743, 1824
ZraI GACGTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.