pycom07g22440

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010964
Physical Location & Seq
Reverse (-)
23781904 .. 23784354
2451 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
ATGGAAAACACATACATGTCACGCAGGCAGCAGCAGCAATCCAGCCCTCGACTTCTCAGAAGCAGAAGTGGAACTTCTTCGACCCCGACCCTAACCCTACCGCAAAACGTTTCACGTTCATGCACTACCACGCCAAACAACAGCCAAAGATTCATCAGCCCCCGGCGCTCGAAATCCACCGTAAAATCAAGAACCAATAAGAATCACGAGGAAGAGAAAGTACACGCACTCAAGCAAAAGAAAGGTGGCCAAGAAGATATCATTATTGGACAACAAGGTACTAATAATTTCGGTCTAAATACTAGTTTTGCTAAGTTGCAGCGAGGGAGTACTAGTCCTAGTCCAAGTCCACGCCGTAGAATATCAGTGTCCGTGCCGTCTCCGTCTGCATGGGCTTTGTCCCCAGGACGGTCTTTGCCGCGCAAGGTGACACCGGATCCCCCAGCGGCAGCGCCTTCGACAAGGGCAGCTAGGGTAAAGTCTAGTGGTGGCGGGGTTGGTGCGGTTTTAAAGTATTTTAGGCTGCAGAAGAAAGTGCCGCCGATACAAGAGGAGGACTTCCAACGGTTTCGGATTTTGCATAATAGGCTGCTGCAGTGGAGGTTTGCAAACGCCAGAGGTGAGGCTGCTAAGCTTGCTACACAGCAAATTTCACAGTTGCTTAATAAATTGTCTTTTATTTTTCTGGATAAATTGTTTAGCGTGTGGCTTAGAACATTGAAAGTGAGAAACTTCATCCTAGACAAAAGAATACAAGTGCAAAAGCTCAAGCATGAGATTAAGGTGTACCAGATTTTGAACCCACAAATTTTCCTACTGAATGAATGGGGAAAATTGGATAAAAAAAATCAAGAATCCATCAGCAGGTTGGTGAGAAAATTGTCAGGAATTTCAAACACAATCCCTTTGGTCAATGATGCTAAGGCAGATGTGGCATCTGTATACGAAGCCATGACCCCGGCCATGGCTGCGATGGAAGGTAATTTAGGAATGGTCTCTAAACTGCTGCCGCAGCTGGAAAAGGTGCTTTACATGGTTACGGAACTATTAATCGCACATAAACAACAGAGAGACCTGGATAGTAATTTAGCCTTGGTTTCTGCACTTGCGGAGGAGGAGGCAAGTGTAAGAGCACATCTCATCCAATTGGAATCAATGTACCTCTTGCCCCCCAACAAATTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

396

Amino Acids

44.41

Weight (kDa)

10.6

Isoelectric Point (pI)

60.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 38 - 357 1.6e-55 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1186
Acc36I ACCTGC 1 cut(s) 855
AccI GTMKAC 1 cut(s) 942
AccII CGCG 1 cut(s) 421
AciI CCGC 8 cut(s) 101, 419, 446, 492, 503, 539, 1010, 1109
AclI AACGTT 1 cut(s) 108
AclWI GGATC 2 cut(s) 431, 444
AcoI YGGCCR 2 cut(s) 247, 960
AcsI RAATTY 4 cut(s) 648, 807, 888, 1178
AfaI GTAC 5 cut(s) 222, 280, 331, 788, 1160
AfiI CCNNNNNNNGG 3 cut(s) 408, 864, 964
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 3 cut(s) 721, 799, 894
AhlI ACTAGT 2 cut(s) 302, 332
AjnI CCWGG 2 cut(s) 403, 1074
AluBI AGCT 4 cut(s) 470, 634, 766, 1015
AluI AGCT 4 cut(s) 470, 634, 766, 1015
Alw21I GWGCWC 1 cut(s) 1135
Alw26I GTCTC 3 cut(s) 384, 1000, 1065
AlwI GGATC 2 cut(s) 431, 444
AoxI GGCC 2 cut(s) 247, 960
ApoI RAATTY 4 cut(s) 648, 807, 888, 1178
AseI ATTAAT 1 cut(s) 1049
Asp700I GAANNNNTTC 1 cut(s) 76
AspLEI GCGC 3 cut(s) 168, 423, 454
AsuC2I CCSGG 2 cut(s) 163, 959
AsuHPI GGTGA 3 cut(s) 439, 632, 883
BalI TGGCCA 1 cut(s) 249
BamHI GGATCC 1 cut(s) 436
BarI GAAGNNNNNNTAC 2 cut(s) 204, 236
BauI CACGAG 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 1135
BccI CCATC 2 cut(s) 866, 967
BceAI ACGGC 2 cut(s) 339, 361
BcgI CGANNNNNNTGC 2 cut(s) 438, 472
BciT130I CCWGG 2 cut(s) 405, 1076
BcnI CCSGG 2 cut(s) 163, 959
BcoDI GTCTC 3 cut(s) 384, 1000, 1065
BcuI ACTAGT 2 cut(s) 302, 332
BfaI CTAG 6 cut(s) 303, 333, 339, 471, 483, 740
BfmI CTRYAG 2 cut(s) 524, 593
BfoI RGCGCY 2 cut(s) 169, 455
BfuAI ACCTGC 1 cut(s) 855
BlpI GCTNAGC 1 cut(s) 630
BmcAI AGTACT 1 cut(s) 331
Bme1390I CCNGG 4 cut(s) 163, 405, 959, 1076
BmiI GGNNCC 1 cut(s) 438
BmrFI CCNGG 4 cut(s) 163, 405, 959, 1076
BmsI GCATC 2 cut(s) 907, 944
BplI GAGNNNNNCTC 2 cut(s) 1122, 1154
Bpu10I CCTNAGC 1 cut(s) 921
Bpu1102I GCTNAGC 1 cut(s) 630
BpuEI CTTGAG 2 cut(s) 215, 752
BpuMI CCSGG 2 cut(s) 163, 959
BsaI GGTCTC 2 cut(s) 1000, 1065
BsaJI CCNNGG 5 cut(s) 161, 403, 957, 963, 1092
BsaWI WCCGGW 1 cut(s) 433
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 3 cut(s) 408, 864, 964
BseBI CCWGG 2 cut(s) 405, 1076
BseDI CCNNGG 5 cut(s) 161, 403, 957, 963, 1092
BseGI GGATG 2 cut(s) 735, 1140
BseLI CCNNNNNNNGG 3 cut(s) 408, 864, 964
BseMII CTCAG 1 cut(s) 70
BseRI GAGGAG 3 cut(s) 566, 1127, 1130
BseYI CCCAGC 1 cut(s) 442
BsgI GTGCAG 1 cut(s) 1086
Bsh1236I CGCG 1 cut(s) 421
BshFI GGCC 2 cut(s) 249, 962
BsiHKAI GWGCWC 1 cut(s) 1135
BsiSI CCGG 3 cut(s) 163, 434, 959
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 3 cut(s) 408, 864, 964
BsmAI GTCTC 3 cut(s) 384, 1000, 1065
BsmBI CGTCTC 1 cut(s) 384
BsmFI GGGAC 1 cut(s) 385
BsnI GGCC 2 cut(s) 249, 962
Bso31I GGTCTC 2 cut(s) 1000, 1065
Bsp1286I GDGCHC 1 cut(s) 1135
Bsp143I GATC 1 cut(s) 436
Bsp1720I GCTNAGC 1 cut(s) 630
Bsp19I CCATGG 1 cut(s) 963
BspACI CCGC 8 cut(s) 101, 419, 446, 492, 503, 539, 1010, 1109
BspANI GGCC 2 cut(s) 249, 962
BspCNI CTCAG 1 cut(s) 69
BspFNI CGCG 1 cut(s) 421
BspLI GGNNCC 1 cut(s) 438
BspMAI CTGCAG 2 cut(s) 528, 597
BspMI ACCTGC 1 cut(s) 855
BspPI GGATC 2 cut(s) 431, 444
BspTNI GGTCTC 2 cut(s) 1000, 1065
BssECI CCNNGG 5 cut(s) 161, 403, 957, 963, 1092
BssMI GATC 1 cut(s) 436
BssNAI GTATAC 1 cut(s) 943
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 2 cut(s) 963, 1092
Bst1107I GTATAC 1 cut(s) 943
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 405, 1076
Bst4CI ACNGT 4 cut(s) 181, 411, 567, 657
Bst6I CTCTTC 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 26, 636
BstDEI CTNAG 5 cut(s) 56, 312, 630, 710, 921
BstDSI CCRYGG 1 cut(s) 963
BstF5I GGATG 2 cut(s) 735, 1140
BstFNI CGCG 1 cut(s) 421
BstH2I RGCGCY 2 cut(s) 169, 455
BstHHI GCGC 3 cut(s) 168, 423, 454
BstKTI GATC 1 cut(s) 439
BstMAI GTCTC 3 cut(s) 384, 1000, 1065
BstMBI GATC 1 cut(s) 436
BstMWI GCNNNNNNNGC 7 cut(s) 34, 165, 586, 635, 932, 968, 1012
BstNI CCWGG 2 cut(s) 405, 1076
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 4 cut(s) 161, 403, 957, 1074
BstSFI CTRYAG 2 cut(s) 524, 593
BstUI CGCG 1 cut(s) 421
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BstZ17I GTATAC 1 cut(s) 943
BsuRI GGCC 2 cut(s) 249, 962
BtgI CCRYGG 1 cut(s) 963
BtgZI GCGATG 1 cut(s) 986
BtsCI GGATG 2 cut(s) 735, 1140
BtsI GCAGTG 1 cut(s) 602
BtsIMutI CAGTG 2 cut(s) 372, 602
BveI ACCTGC 1 cut(s) 855
Cac8I GCNNGC 2 cut(s) 26, 636
CfoI GCGC 3 cut(s) 168, 423, 454
Csp6I GTAC 5 cut(s) 221, 279, 330, 787, 1159
CviAII CATG 7 cut(s) 16, 120, 390, 773, 952, 964, 1033
CviQI GTAC 5 cut(s) 221, 279, 330, 787, 1159
DdeI CTNAG 5 cut(s) 56, 312, 630, 710, 921
DpnI GATC 1 cut(s) 438
DpnII GATC 1 cut(s) 436
DraI TTTAAA 1 cut(s) 510
EaeI YGGCCR 2 cut(s) 247, 960
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco130I CCWWGG 2 cut(s) 963, 1092
Eco31I GGTCTC 2 cut(s) 1000, 1065
Eco32I GATATC 1 cut(s) 259
EcoRII CCWGG 2 cut(s) 403, 1074
EcoRV GATATC 1 cut(s) 259
EcoT14I CCWWGG 2 cut(s) 963, 1092
ErhI CCWWGG 2 cut(s) 963, 1092
Esp3I CGTCTC 1 cut(s) 384
FaeI CATG 7 cut(s) 19, 123, 393, 776, 955, 967, 1036
FalI AAGNNNNNCTT 2 cut(s) 58, 90
FaqI GGGAC 1 cut(s) 385
FatI CATG 7 cut(s) 15, 119, 389, 772, 951, 963, 1032
FauI CCCGC 1 cut(s) 485
FblI GTMKAC 1 cut(s) 942
FokI GGATG 2 cut(s) 722, 1127
FspBI CTAG 6 cut(s) 303, 333, 339, 471, 483, 740
GlaI GCGC 3 cut(s) 167, 422, 453
GsaI CCCAGC 1 cut(s) 446
HaeII RGCGCY 2 cut(s) 169, 455
HaeIII GGCC 2 cut(s) 249, 962
HapII CCGG 3 cut(s) 163, 434, 959
HhaI GCGC 3 cut(s) 168, 423, 454
Hin1II CATG 7 cut(s) 19, 123, 393, 776, 955, 967, 1036
Hin6I GCGC 3 cut(s) 166, 421, 452
HinP1I GCGC 3 cut(s) 166, 421, 452
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 4 cut(s) 150, 202, 854, 1151
HpaII CCGG 3 cut(s) 163, 434, 959
HphI GGTGA 3 cut(s) 439, 632, 883
Hpy166II GTNNAC 4 cut(s) 223, 350, 787, 943
Hpy188I TCNGA 2 cut(s) 59, 573
Hpy188III TCNNGA 5 cut(s) 189, 206, 686, 851, 885
Hpy8I GTNNAC 4 cut(s) 223, 350, 787, 943
HpyAV CCTTC 2 cut(s) 465, 971
HpyCH4III ACNGT 4 cut(s) 181, 411, 567, 657
HpyCH4IV ACGT 2 cut(s) 108, 115
HpyCH4V TGCA 9 cut(s) 123, 319, 389, 526, 580, 595, 608, 760, 1103
HpyF10VI GCNNNNNNNGC 7 cut(s) 34, 165, 586, 635, 932, 968, 1012
HpyF3I CTNAG 5 cut(s) 56, 312, 630, 710, 921
HpySE526I ACGT 2 cut(s) 108, 115
Hsp92II CATG 7 cut(s) 19, 123, 393, 776, 955, 967, 1036
HspAI GCGC 3 cut(s) 166, 421, 452
Kzo9I GATC 1 cut(s) 436
LweI GCATC 2 cut(s) 907, 944
MaeI CTAG 6 cut(s) 303, 333, 339, 471, 483, 740
MaeII ACGT 2 cut(s) 108, 115
MaeIII GTNAC 3 cut(s) 18, 427, 1036
MalI GATC 1 cut(s) 438
MboI GATC 1 cut(s) 436
MboII GAAGA 4 cut(s) 69, 224, 266, 541
MfeI CAATTG 1 cut(s) 1145
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 1 cut(s) 1135
MlsI TGGCCA 1 cut(s) 249
MluNI TGGCCA 1 cut(s) 249
MmeI TCCRAC 1 cut(s) 586
Mox20I TGGCCA 1 cut(s) 249
MroXI GAANNNNTTC 1 cut(s) 76
MscI TGGCCA 1 cut(s) 249
MseI TTAA 4 cut(s) 509, 663, 780, 1049
MslI CAYNNNNRTG 2 cut(s) 14, 118
Msp20I TGGCCA 1 cut(s) 249
MspA1I CMGCKG 2 cut(s) 446, 1015
MspI CCGG 3 cut(s) 163, 434, 959
MspR9I CCNGG 4 cut(s) 163, 405, 959, 1076
MunI CAATTG 1 cut(s) 1145
MvaI CCWGG 2 cut(s) 405, 1076
MvnI CGCG 1 cut(s) 421
MwoI GCNNNNNNNGC 7 cut(s) 34, 165, 586, 635, 932, 968, 1012
NciI CCSGG 2 cut(s) 163, 959
NcoI CCATGG 1 cut(s) 963
NdeII GATC 1 cut(s) 436
NlaIII CATG 7 cut(s) 19, 123, 393, 776, 955, 967, 1036
NlaIV GGNNCC 1 cut(s) 438
NmuCI GTSAC 2 cut(s) 18, 427
NspI RCATGY 1 cut(s) 19
PciI ACATGT 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 76
PfeI GAWTC 4 cut(s) 150, 202, 854, 1151
PscI ACATGT 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 1049
PsiI TTATAA 1 cut(s) 1186
Psp1406I AACGTT 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 403, 1074
PspFI CCCAGC 1 cut(s) 442
PspGI CCWGG 2 cut(s) 403, 1074
PspN4I GGNNCC 1 cut(s) 438
PstI CTGCAG 2 cut(s) 528, 597
PsuI RGATCY 1 cut(s) 436
PvuII CAGCTG 1 cut(s) 1015
RsaI GTAC 5 cut(s) 222, 280, 331, 788, 1160
RsaNI GTAC 5 cut(s) 221, 279, 330, 787, 1159
RseI CAYNNNNRTG 2 cut(s) 14, 118
SaqAI TTAA 4 cut(s) 509, 663, 780, 1049
Sau3AI GATC 1 cut(s) 436
ScaI AGTACT 1 cut(s) 331
ScrFI CCNGG 4 cut(s) 163, 405, 959, 1076
SduI GDGCHC 1 cut(s) 1135
SfaNI GCATC 2 cut(s) 907, 944
SfcI CTRYAG 2 cut(s) 524, 593
SmiMI CAYNNNNRTG 2 cut(s) 14, 118
SmlI CTYRAG 2 cut(s) 230, 767
SmoI CTYRAG 2 cut(s) 230, 767
SpeI ACTAGT 2 cut(s) 302, 332
SsiI CCGC 8 cut(s) 101, 419, 446, 492, 503, 539, 1010, 1109
SspMI CTAG 6 cut(s) 303, 333, 339, 471, 483, 740
StyD4I CCNGG 4 cut(s) 161, 403, 957, 1074
StyI CCWWGG 2 cut(s) 963, 1092
TaaI ACNGT 4 cut(s) 181, 411, 567, 657
TaiI ACGT 2 cut(s) 111, 118
TaqI TCGA 4 cut(s) 49, 80, 170, 458
TaqII GACCGA 1 cut(s) 281
TatI WGTACW 2 cut(s) 220, 329
TauI GCSGC 4 cut(s) 421, 449, 541, 1012
TfiI GAWTC 4 cut(s) 150, 202, 854, 1151
Tru1I TTAA 4 cut(s) 509, 663, 780, 1049
Tru9I TTAA 4 cut(s) 509, 663, 780, 1049
TscAI CASTG 2 cut(s) 372, 602
TseFI GTSAC 2 cut(s) 18, 427
Tsp45I GTSAC 2 cut(s) 18, 427
TspDTI ATGAA 4 cut(s) 108, 142, 724, 837
TspGWI ACGGA 3 cut(s) 361, 372, 1055
TspRI CASTG 2 cut(s) 372, 602
VspI ATTAAT 1 cut(s) 1049
XapI RAATTY 4 cut(s) 648, 807, 888, 1178
XceI RCATGY 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 2 cut(s) 865, 970
XmiI GTMKAC 1 cut(s) 942
XmnI GAANNNNTTC 1 cut(s) 76
XspI CTAG 6 cut(s) 303, 333, 339, 471, 483, 740
ZrmI AGTACT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.