pycom07g24350

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010596
Physical Location & Seq
Forward (+)
25086700 .. 25087128
429 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g24350.2

Sequence Viewer

Length: 261 bp
ATGTCTACATATCCAAGAACTTATGGCTTCATTCATGCTGATTCAGTGTTTAGCCTGTATCAGGATAGATGCAATGCGGATGACATTTTGCTGGAGATGGACAGAATTCTGAGGCCAGAGGGGAGCGTGATCATTCGAGATGACGTAGATGTGTTGATTGTAGACCATGAAGACGGTCCTCACGAGAGGGAAAAACTGCTGTTCACCGTGAAAAAGTATTGGACAGCTCCTGCTGCAGCTGAGGATGAATCTTCCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

87

Amino Acids

9.87

Weight (kDa)

4.52

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 5, 162
AciI CCGC 1 cut(s) 77
AcsI RAATTY 1 cut(s) 105
AfiI CCNNNNNNNGG 1 cut(s) 61
AluBI AGCT 2 cut(s) 227, 239
AluI AGCT 2 cut(s) 227, 239
AlwNI CAGNNNCTG 1 cut(s) 230
AoxI GGCC 1 cut(s) 113
ApeKI GCWGC 2 cut(s) 233, 236
ApoI RAATTY 1 cut(s) 105
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 196
AvaII GGWCC 1 cut(s) 176
BauI CACGAG 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 220, 248
BccI CCATC 1 cut(s) 91
BclI TGATCA 1 cut(s) 129
BfaI CTAG 1 cut(s) 259
BfmI CTRYAG 1 cut(s) 234
BisI GCNGC 2 cut(s) 234, 237
BlsI GCNGC 2 cut(s) 235, 238
Bme18I GGWCC 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 176
BmsI GCATC 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 113
Bpu10I CCTNAGC 1 cut(s) 240
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse3DI GCAATG 1 cut(s) 79
BseGI GGATG 2 cut(s) 85, 250
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 1 cut(s) 79
BseMII CTCAG 2 cut(s) 101, 231
BseXI GCAGC 2 cut(s) 220, 248
BshFI GGCC 1 cut(s) 115
BslI CCNNNNNNNGG 1 cut(s) 61
BsnI GGCC 1 cut(s) 115
Bsp143I GATC 1 cut(s) 129
BspACI CCGC 1 cut(s) 77
BspANI GGCC 1 cut(s) 115
BspCNI CTCAG 2 cut(s) 102, 232
BspMAI CTGCAG 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 79
BssMI GATC 1 cut(s) 129
BssSI CACGAG 1 cut(s) 182
Bst2BI CACGAG 1 cut(s) 182
Bst4CI ACNGT 2 cut(s) 176, 208
BstDEI CTNAG 2 cut(s) 110, 240
BstENI CCTNNNNNAGG 1 cut(s) 59
BstF5I GGATG 2 cut(s) 85, 250
BstKTI GATC 1 cut(s) 132
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 2 cut(s) 220, 248
BstV2I GAAGAC 1 cut(s) 177
BsuRI GGCC 1 cut(s) 115
BtsCI GGATG 2 cut(s) 85, 250
BtsIMutI CAGTG 1 cut(s) 51
CaiI CAGNNNCTG 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 176
CviAII CATG 2 cut(s) 35, 167
CviJI RGCY 5 cut(s) 27, 54, 115, 227, 239
CviKI_1 RGCY 5 cut(s) 27, 54, 115, 227, 239
DdeI CTNAG 2 cut(s) 110, 240
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 176
EcoNI CCTNNNNNAGG 1 cut(s) 59
EcoRI GAATTC 1 cut(s) 105
FaeI CATG 2 cut(s) 38, 170
FaiI YATR 4 cut(s) 10, 24, 36, 168
FatI CATG 2 cut(s) 34, 166
FbaI TGATCA 1 cut(s) 129
FblI GTMKAC 2 cut(s) 5, 162
Fnu4HI GCNGC 2 cut(s) 234, 237
FokI GGATG 2 cut(s) 92, 257
Fsp4HI GCNGC 2 cut(s) 234, 237
FspBI CTAG 1 cut(s) 259
GluI GCNGC 2 cut(s) 234, 237
GsuI CTGGAG 1 cut(s) 113
HaeIII GGCC 1 cut(s) 115
Hin1II CATG 2 cut(s) 38, 170
HinfI GANTC 2 cut(s) 41, 248
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 3 cut(s) 6, 163, 204
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 3 cut(s) 62, 137, 182
Hpy8I GTNNAC 3 cut(s) 6, 163, 204
HpyCH4III ACNGT 2 cut(s) 176, 208
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 2 cut(s) 72, 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 2 cut(s) 110, 240
HpySE526I ACGT 1 cut(s) 144
Hsp92II CATG 2 cut(s) 38, 170
Ksp22I TGATCA 1 cut(s) 129
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 2 cut(s) 123, 232
LpnPI CCDG 5 cut(s) 47, 68, 77, 129, 243
Lsp1109I GCAGC 2 cut(s) 220, 248
LweI GCATC 1 cut(s) 59
MaeI CTAG 1 cut(s) 259
MaeII ACGT 1 cut(s) 144
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 2 cut(s) 182, 243
MluCI AATT 1 cut(s) 105
MnlI CCTC 5 cut(s) 105, 112, 180, 189, 235
MspA1I CMGCKG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 233
NdeII GATC 1 cut(s) 129
NlaIII CATG 2 cut(s) 38, 170
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PfeI GAWTC 2 cut(s) 41, 248
PkrI GCNGC 2 cut(s) 235, 238
PspPI GGNCC 1 cut(s) 176
PstI CTGCAG 1 cut(s) 238
PstNI CAGNNNCTG 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 239
SatI GCNGC 2 cut(s) 234, 237
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 1 cut(s) 176
SetI ASST 3 cut(s) 147, 229, 241
SfaNI GCATC 1 cut(s) 59
SfcI CTRYAG 1 cut(s) 234
SinI GGWCC 1 cut(s) 176
Sse9I AATT 1 cut(s) 105
SsiI CCGC 1 cut(s) 77
SspMI CTAG 1 cut(s) 259
TaaI ACNGT 2 cut(s) 176, 208
TaiI ACGT 1 cut(s) 147
TaqI TCGA 1 cut(s) 136
TasI AATT 1 cut(s) 105
TfiI GAWTC 2 cut(s) 41, 248
TscAI CASTG 1 cut(s) 51
TseI GCWGC 2 cut(s) 233, 236
TspDTI ATGAA 4 cut(s) 19, 23, 183, 261
TspRI CASTG 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 176
XagI CCTNNNNNAGG 1 cut(s) 59
XapI RAATTY 1 cut(s) 105
XmiI GTMKAC 2 cut(s) 5, 162
XspI CTAG 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.