Rh5DG486700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010596
Physical Location & Seq
Forward (+)
76578128 .. 76578388
261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG486700.1

Sequence Viewer

Length: 261 bp
ATGCAACTCGAAAAAGAAGAAGAAAGACTTACCAAGCTGCCGAGATCAGAGGAGTATTCAGTTAGGGAAGTGAAGATGAAAGTGAGGGAGACGAAGAAGATGATGACTGAGGAGGTAATGGTGAGCGCCAGTAATCTGAAAGACGAGCTTCGCCGGAAGTCATCAACTCATGGCCAATCTGGAAGGGAAAGGCAAAGACGCGACGACGCCCAGACTTGCGGCGCCAAGACTTTGCTTAATACCTGGCCTCACCGCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

86

Amino Acids

10.16

Weight (kDa)

9.66

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 221
AccII CGCG 2 cut(s) 201, 255
AciI CCGC 2 cut(s) 219, 253
AcoI YGGCCR 1 cut(s) 172
AcyI GRCGYC 2 cut(s) 207, 222
AjnI CCWGG 1 cut(s) 242
AluBI AGCT 2 cut(s) 37, 148
AluI AGCT 2 cut(s) 37, 148
Alw26I GTCTC 1 cut(s) 83
AoxI GGCC 2 cut(s) 172, 245
ApeKI GCWGC 1 cut(s) 37
AspLEI GCGC 3 cut(s) 128, 224, 257
AsuHPI GGTGA 2 cut(s) 133, 242
BalI TGGCCA 1 cut(s) 174
BanI GGYRCC 1 cut(s) 221
BbvI GCAGC 1 cut(s) 24
BciT130I CCWGG 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 83
BfoI RGCGCY 2 cut(s) 129, 225
BisI GCNGC 2 cut(s) 38, 220
BlsI GCNGC 2 cut(s) 39, 221
Bme1390I CCNGG 1 cut(s) 244
BmiI GGNNCC 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 244
BsaHI GRCGYC 2 cut(s) 207, 222
Bse1I ACTGG 1 cut(s) 129
BseBI CCWGG 1 cut(s) 244
BseMII CTCAG 1 cut(s) 99
BseNI ACTGG 1 cut(s) 129
BseRI GAGGAG 2 cut(s) 65, 125
BseXI GCAGC 1 cut(s) 24
Bsh1236I CGCG 2 cut(s) 201, 255
BshFI GGCC 2 cut(s) 174, 247
BshNI GGYRCC 1 cut(s) 221
BsiSI CCGG 1 cut(s) 154
BsmAI GTCTC 1 cut(s) 83
BsmBI CGTCTC 1 cut(s) 83
BsnI GGCC 2 cut(s) 174, 247
Bsp143I GATC 1 cut(s) 44
BspACI CCGC 2 cut(s) 219, 253
BspANI GGCC 2 cut(s) 174, 247
BspCNI CTCAG 1 cut(s) 100
BspFNI CGCG 2 cut(s) 201, 255
BspLI GGNNCC 1 cut(s) 223
BspT107I GGYRCC 1 cut(s) 221
BsrI ACTGG 1 cut(s) 129
BssMI GATC 1 cut(s) 44
BssNI GRCGYC 2 cut(s) 207, 222
Bst2UI CCWGG 1 cut(s) 244
BstACI GRCGYC 2 cut(s) 207, 222
BstDEI CTNAG 1 cut(s) 108
BstFNI CGCG 2 cut(s) 201, 255
BstH2I RGCGCY 2 cut(s) 129, 225
BstHHI GCGC 3 cut(s) 128, 224, 257
BstKTI GATC 1 cut(s) 47
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 1 cut(s) 44
BstNI CCWGG 1 cut(s) 244
BstSCI CCNGG 1 cut(s) 242
BstUI CGCG 2 cut(s) 201, 255
BstV1I GCAGC 1 cut(s) 24
BsuRI GGCC 2 cut(s) 174, 247
CfoI GCGC 3 cut(s) 128, 224, 257
CseI GACGC 2 cut(s) 207, 215
CviAII CATG 1 cut(s) 170
CviJI RGCY 4 cut(s) 37, 148, 174, 247
CviKI_1 RGCY 4 cut(s) 37, 148, 174, 247
DdeI CTNAG 1 cut(s) 108
DinI GGCGCC 1 cut(s) 223
DpnI GATC 1 cut(s) 46
DpnII GATC 1 cut(s) 44
EaeI YGGCCR 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 242
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 1 cut(s) 173
FaiI YATR 1 cut(s) 171
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 132, 164
FatI CATG 1 cut(s) 169
Fnu4HI GCNGC 2 cut(s) 38, 220
Fsp4HI GCNGC 2 cut(s) 38, 220
GlaI GCGC 3 cut(s) 127, 223, 256
GluI GCNGC 2 cut(s) 38, 220
HaeII RGCGCY 2 cut(s) 129, 225
HaeIII GGCC 2 cut(s) 174, 247
HapII CCGG 1 cut(s) 154
HgaI GACGC 2 cut(s) 207, 215
HhaI GCGC 3 cut(s) 128, 224, 257
Hin1I GRCGYC 2 cut(s) 207, 222
Hin1II CATG 1 cut(s) 173
Hin6I GCGC 3 cut(s) 126, 222, 255
HinP1I GCGC 3 cut(s) 126, 222, 255
HpaII CCGG 1 cut(s) 154
HphI GGTGA 2 cut(s) 133, 242
Hpy188I TCNGA 2 cut(s) 49, 138
Hpy188III TCNNGA 1 cut(s) 180
Hpy99I CGWCG 2 cut(s) 206, 209
HpyAV CCTTC 1 cut(s) 177
HpyCH4V TGCA 1 cut(s) 4
HpyF3I CTNAG 1 cut(s) 108
Hsp92I GRCGYC 2 cut(s) 207, 222
Hsp92II CATG 1 cut(s) 173
HspAI GCGC 3 cut(s) 126, 222, 255
KasI GGCGCC 1 cut(s) 221
Kzo9I GATC 1 cut(s) 44
LpnPI CCDG 6 cut(s) 142, 165, 167, 224, 229, 256
Lsp1109I GCAGC 1 cut(s) 24
MalI GATC 1 cut(s) 46
MboI GATC 1 cut(s) 44
MboII GAAGA 5 cut(s) 29, 32, 85, 106, 109
MlsI TGGCCA 1 cut(s) 174
MluNI TGGCCA 1 cut(s) 174
Mly113I GGCGCC 1 cut(s) 222
MnlI CCTC 5 cut(s) 43, 78, 103, 106, 258
Mox20I TGGCCA 1 cut(s) 174
MscI TGGCCA 1 cut(s) 174
MseI TTAA 1 cut(s) 237
Msp20I TGGCCA 1 cut(s) 174
MspI CCGG 1 cut(s) 154
MspR9I CCNGG 1 cut(s) 244
MvaI CCWGG 1 cut(s) 244
MvnI CGCG 2 cut(s) 201, 255
NarI GGCGCC 1 cut(s) 222
NdeII GATC 1 cut(s) 44
NlaIII CATG 1 cut(s) 173
NlaIV GGNNCC 1 cut(s) 223
NmeAIII GCCGAG 1 cut(s) 66
PkrI GCNGC 2 cut(s) 39, 221
PluTI GGCGCC 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 2 cut(s) 38, 220
Sau3AI GATC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 244
SetI ASST 4 cut(s) 39, 117, 150, 245
SfoI GGCGCC 1 cut(s) 223
SsiI CCGC 2 cut(s) 219, 253
SspDI GGCGCC 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 242
TaqI TCGA 1 cut(s) 9
TauI GCSGC 1 cut(s) 222
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TseI GCWGC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.