RchiOBHm_Chr4g0393521

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010596
Physical Location & Seq
Forward (+)
9062713 .. 9063836
1124 bp
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UTR
Exon/CDS
Intron
PRQ36611

Sequence Viewer

Length: 315 bp
ATGGATATGAAAGCACACATGGGATCATTTGCAGCTGCTCTAAAGGACAAGGATGTTTGGGTGATGCATGTCGTCCCTGAAGATGGACCAAACACACTAAAGCTGATATACGACAGAGGGCTTATAGGCAGTATTCACAGCTGGTGTGAAGCCTATTCAACATACCCCCGTACTTATGATTTGCTCCATGCTTGGACCGTCTTCTCTGACTTGGAAAAGAAAGAATGCAGTGCTGAGGATCTGTTGCTTGAGATGGATCGCATACTCAGGCCAACTGGATTTATAATTATCCGTGACAAACAGTCAAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

104

Amino Acids

11.89

Weight (kDa)

5.45

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 1 - 101 1.2e-52 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 284
AclWI GGATC 3 cut(s) 31, 246, 264
AcuI CTGAAG 1 cut(s) 99
AfaI GTAC 1 cut(s) 172
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 1 cut(s) 159
AhdI GACNNNNNGTC 1 cut(s) 301
AluBI AGCT 3 cut(s) 35, 103, 141
AluI AGCT 3 cut(s) 35, 103, 141
AlwI GGATC 3 cut(s) 31, 246, 264
AoxI GGCC 1 cut(s) 269
ApeKI GCWGC 2 cut(s) 32, 35
AspS9I GGNCC 2 cut(s) 86, 195
AsuHPI GGTGA 1 cut(s) 73
AvaII GGWCC 2 cut(s) 86, 195
BbsI GAAGAC 1 cut(s) 193
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 2 cut(s) 22, 44
BccI CCATC 2 cut(s) 77, 247
BisI GCNGC 2 cut(s) 33, 36
BlsI GCNGC 2 cut(s) 34, 37
Bme18I GGWCC 2 cut(s) 86, 195
BmeRI GACNNNNNGTC 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 86, 195
BmsI GCATC 1 cut(s) 54
BpiI GAAGAC 1 cut(s) 193
Bpu10I CCTNAGC 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 269
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 58
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 2 cut(s) 225, 280
BseNI ACTGG 1 cut(s) 280
BseXI GCAGC 2 cut(s) 22, 44
BshFI GGCC 1 cut(s) 271
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 1 cut(s) 271
Bsp143I GATC 3 cut(s) 23, 238, 256
BspANI GGCC 1 cut(s) 271
BspCNI CTCAG 2 cut(s) 226, 279
BspPI GGATC 3 cut(s) 31, 246, 264
BsrI ACTGG 1 cut(s) 280
BssMI GATC 3 cut(s) 23, 238, 256
Bst4CI ACNGT 2 cut(s) 199, 303
BstDEI CTNAG 2 cut(s) 234, 266
BstF5I GGATG 1 cut(s) 58
BstKTI GATC 3 cut(s) 26, 241, 259
BstMBI GATC 3 cut(s) 23, 238, 256
BstNSI RCATGY 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 22, 44
BstV2I GAAGAC 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 1 cut(s) 271
BtsCI GGATG 1 cut(s) 58
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 86, 195
Csp6I GTAC 1 cut(s) 171
CviAII CATG 3 cut(s) 19, 68, 188
CviJI RGCY 6 cut(s) 35, 103, 121, 141, 152, 271
CviKI_1 RGCY 6 cut(s) 35, 103, 121, 141, 152, 271
CviQI GTAC 1 cut(s) 171
DdeI CTNAG 2 cut(s) 234, 266
DpnI GATC 3 cut(s) 25, 240, 258
DpnII GATC 3 cut(s) 23, 238, 256
DriI GACNNNNNGTC 1 cut(s) 301
Eam1105I GACNNNNNGTC 1 cut(s) 301
Eco47I GGWCC 2 cut(s) 86, 195
Eco57I CTGAAG 1 cut(s) 99
EcoT22I ATGCAT 1 cut(s) 69
FaeI CATG 3 cut(s) 22, 71, 191
FaqI GGGAC 1 cut(s) 59
FatI CATG 3 cut(s) 18, 67, 187
Fnu4HI GCNGC 2 cut(s) 33, 36
FokI GGATG 1 cut(s) 65
Fsp4HI GCNGC 2 cut(s) 33, 36
GluI GCNGC 2 cut(s) 33, 36
HaeIII GGCC 1 cut(s) 271
Hin1II CATG 3 cut(s) 22, 71, 191
HphI GGTGA 1 cut(s) 73
Hpy188I TCNGA 1 cut(s) 208
HpyCH4III ACNGT 2 cut(s) 199, 303
HpyCH4V TGCA 3 cut(s) 32, 67, 228
HpyF3I CTNAG 2 cut(s) 234, 266
Hsp92II CATG 3 cut(s) 22, 71, 191
Kzo9I GATC 3 cut(s) 23, 238, 256
LmnI GCTCC 1 cut(s) 189
LpnPI CCDG 4 cut(s) 90, 127, 253, 261
Lsp1109I GCAGC 2 cut(s) 22, 44
LweI GCATC 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 293
MalI GATC 3 cut(s) 25, 240, 258
MboI GATC 3 cut(s) 23, 238, 256
MboII GAAGA 2 cut(s) 92, 193
MflI RGATCY 1 cut(s) 238
MluCI AATT 1 cut(s) 285
MnlI CCTC 2 cut(s) 110, 229
Mph1103I ATGCAT 1 cut(s) 69
MspA1I CMGCKG 2 cut(s) 35, 141
Mva1269I GAATGC 1 cut(s) 230
NdeII GATC 3 cut(s) 23, 238, 256
NlaIII CATG 3 cut(s) 22, 71, 191
NmuCI GTSAC 1 cut(s) 293
NsiI ATGCAT 1 cut(s) 69
NspI RCATGY 1 cut(s) 71
PctI GAATGC 1 cut(s) 230
PkrI GCNGC 2 cut(s) 34, 37
PsiI TTATAA 1 cut(s) 284
PspPI GGNCC 2 cut(s) 86, 195
PsuI RGATCY 1 cut(s) 238
PvuII CAGCTG 2 cut(s) 35, 141
RsaI GTAC 1 cut(s) 172
RsaNI GTAC 1 cut(s) 171
SatI GCNGC 2 cut(s) 33, 36
Sau3AI GATC 3 cut(s) 23, 238, 256
Sau96I GGNCC 2 cut(s) 86, 195
SetI ASST 3 cut(s) 37, 105, 143
SfaNI GCATC 1 cut(s) 54
SinI GGWCC 2 cut(s) 86, 195
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 1 cut(s) 285
TaaI ACNGT 2 cut(s) 199, 303
TasI AATT 1 cut(s) 285
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 2 cut(s) 32, 35
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 2 cut(s) 86, 195
XceI RCATGY 1 cut(s) 71
Zsp2I ATGCAT 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.