pycom08g06530

protein KIAA0930 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004749
Physical Location & Seq
Reverse (-)
5089012 .. 5092426
3415 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
ATGCTTCCAATCGAAATGCTCGGACATGGAGAAGACACCCCGTCCAGGTTCGAATTGCTGAGTATGGTGAAGAAGCACTCGAGCTTATTGGGGAAAACAGTGATCGTCGCGGACGAGGACGACACTTCCGATGTCGAATTGGACCACAGATTCTGGCACGACGTGTTGGATTTGTACTTCGTTCGCGGTAAAGATTCCAGAGGACGCCAGGACGATGATCTCGTCTTCTTCGTCAGAAAATTGAGCTCTTATGGTTATGGATTCAATGACGGCAAGGAAGGTGTTTCTCCGTACTTCGTACGCAGGTGGGCACCTAAGTTGGATGACTTGATCGGGGAAAGTAATGTGGATGTGGATTGGAGGCGGTCGTTTTACTTGAACTTGATTGCTCACACTTCTTTTACTGTAACAGTGGCTATTTGCAGTCATCAGGACCTCCGGAATCATCAAGCTGAGCAAGGTATACCATTATCTCCAATATATAAGGTTGTAAAGACTGTTTATGCATCTCCAAGCCGTGTCAATTTTCAGTTGGACTCAAAAAAGGAAGTGGAGACAACACCTGCTTATCCAGATATTTGTTTTGCAATCGATGACTTCGACTCCACTTTTGATGCTGTGGTCTTGACTGAAACAGACCATTGCTACTGCGTAATTCTCAATGCACACGATGGGGCAGCATTTCCGAGGGAAAAGGAGTCTAATGATTGCAGTTCTAGTGATAGTTCCTCTCTGAGTGTTGAGACTAACTCCGCAAAGATGAAAAATACAAAGCTGACTCTTTTTTCAGGATTCGTCAGCTATCAAATGGTTCGAGACGCATATGATGCTGGCAAGTCTCGATTTGGGAACCTTCTTGGTCATTCCCCTGGTAAAACAGACAAACTTTACATGAAAGGTCCCGGAGGACGTGGGGAAGTTGAAGTAGCTGTTTCTAGGGTTGCAGATCAAAGCCAACATATAGATCTGGGTCCTTTTTCACCAGTTATATCCAAAAGAGGATTTGGAATTGGCTCCATTGTTCGTAAAGCAGCTTCTGTTGCGTCTGTGGCTGCGAAGAATGCCTATGCAGCTGCTTCTTCTACCCATAGTTTTGATGATGAGATGGTACCTCTCAAATGCTGCTTAATGTCCATTTCATTACCCTGGGAACAGATTGCATATGATCTTCTCTTTAAGGGTACTCCTCCAGTGACGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2045

Protein Analysis

401

Amino Acids

44.3

Weight (kDa)

5.56

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2045 PF09741 48 - 314 1.2e-76 Uncharacterized conserved protein (DUF2045)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 294, 571
Acc36I ACCTGC 2 cut(s) 294, 571
Acc65I GGTACC 1 cut(s) 1108
AccB1I GGYRCC 2 cut(s) 310, 1108
AccI GTMKAC 1 cut(s) 463
AccII CGCG 2 cut(s) 110, 186
AccIII TCCGGA 1 cut(s) 438
AciI CCGC 4 cut(s) 110, 186, 364, 753
AcyI GRCGYC 1 cut(s) 205
AdeI CACNNNGTG 1 cut(s) 163
AfaI GTAC 5 cut(s) 176, 293, 300, 1110, 1183
AfiI CCNNNNNNNGG 1 cut(s) 45
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 3 cut(s) 265, 379, 923
AhdI GACNNNNNGTC 1 cut(s) 40
AjiI CACGTC 2 cut(s) 163, 911
AjnI CCWGG 4 cut(s) 44, 207, 868, 1145
AluBI AGCT 8 cut(s) 84, 246, 452, 775, 801, 929, 1034, 1073
AluI AGCT 8 cut(s) 84, 246, 452, 775, 801, 929, 1034, 1073
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 4 cut(s) 548, 737, 810, 843
AlwNI CAGNNNCTG 2 cut(s) 153, 1037
Ama87I CYCGRG 1 cut(s) 79
Aor13HI TCCGGA 1 cut(s) 438
ApeKI GCWGC 6 cut(s) 677, 1031, 1052, 1070, 1073, 1122
ArsI GACNNNNNNTTYG 2 cut(s) 593, 625
Asp718I GGTACC 1 cut(s) 1108
AspS9I GGNCC 4 cut(s) 142, 433, 899, 971
AsuC2I CCSGG 1 cut(s) 903
AsuHPI GGTGA 2 cut(s) 79, 972
AsuII TTCGAA 1 cut(s) 51
AvaI CYCGRG 1 cut(s) 79
AvaII GGWCC 4 cut(s) 142, 433, 899, 971
BaeGI GKGCMC 1 cut(s) 313
BanI GGYRCC 2 cut(s) 310, 1108
BanII GRGCYC 1 cut(s) 248
BarI GAAGNNNNNNTAC 2 cut(s) 1018, 1050
BbsI GAAGAC 2 cut(s) 39, 217
Bbv12I GWGCWC 1 cut(s) 248
BbvI GCAGC 6 cut(s) 689, 1039, 1043, 1060, 1082, 1109
BccI CCATC 2 cut(s) 665, 1099
BceAI ACGGC 2 cut(s) 286, 501
BciT130I CCWGG 4 cut(s) 46, 209, 870, 1147
BcnI CCSGG 1 cut(s) 903
BcoDI GTCTC 4 cut(s) 548, 737, 810, 843
BfaI CTAG 2 cut(s) 717, 936
BfuAI ACCTGC 2 cut(s) 294, 571
BglII AGATCT 1 cut(s) 964
BisI GCNGC 6 cut(s) 678, 1032, 1053, 1071, 1074, 1123
BlpI GCTNAGC 1 cut(s) 453
BlsI GCNGC 6 cut(s) 679, 1033, 1054, 1072, 1075, 1124
Bme1390I CCNGG 5 cut(s) 46, 209, 870, 903, 1147
Bme18I GGWCC 4 cut(s) 142, 433, 899, 971
BmeRI GACNNNNNGTC 1 cut(s) 40
BmeT110I CYCGRG 1 cut(s) 79
BmgBI CACGTC 2 cut(s) 163, 911
BmgT120I GGNCC 4 cut(s) 142, 433, 899, 971
BmiI GGNNCC 6 cut(s) 312, 851, 901, 972, 1015, 1110
BmrFI CCNGG 5 cut(s) 46, 209, 870, 903, 1147
BmsI GCATC 3 cut(s) 515, 604, 817
BpiI GAAGAC 2 cut(s) 39, 217
BplI GAGNNNNNCTC 2 cut(s) 734, 766
BpmI CTGGAG 1 cut(s) 1173
Bpu1102I GCTNAGC 1 cut(s) 453
Bpu14I TTCGAA 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 903
Bsa29I ATCGAT 1 cut(s) 591
BsaHI GRCGYC 1 cut(s) 205
BsaJI CCNNGG 4 cut(s) 686, 868, 1145, 1146
BsaWI WCCGGW 1 cut(s) 438
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse1I ACTGG 2 cut(s) 983, 1190
Bse3DI GCAATG 1 cut(s) 640
BseAI TCCGGA 1 cut(s) 438
BseBI CCWGG 4 cut(s) 46, 209, 870, 1147
BseCI ATCGAT 1 cut(s) 591
BseDI CCNNGG 4 cut(s) 686, 868, 1145, 1146
BseGI GGATG 2 cut(s) 328, 355
BseLI CCNNNNNNNGG 1 cut(s) 45
BseMI GCAATG 1 cut(s) 640
BseMII CTCAG 3 cut(s) 50, 444, 725
BseNI ACTGG 2 cut(s) 983, 1190
BseRI GAGGAG 1 cut(s) 1176
BseSI GKGCMC 1 cut(s) 313
BseXI GCAGC 6 cut(s) 689, 1039, 1043, 1060, 1082, 1109
Bsh1236I CGCG 2 cut(s) 110, 186
Bsh1285I CGRYCG 1 cut(s) 368
BshNI GGYRCC 2 cut(s) 310, 1108
BshVI ATCGAT 1 cut(s) 591
BsiEI CGRYCG 1 cut(s) 368
BsiHKAI GWGCWC 1 cut(s) 248
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 2 cut(s) 439, 903
BsiWI CGTACG 1 cut(s) 298
BslFI GGGAC 1 cut(s) 885
BslI CCNNNNNNNGG 1 cut(s) 45
BsmAI GTCTC 4 cut(s) 548, 737, 810, 843
BsmBI CGTCTC 1 cut(s) 810
BsmFI GGGAC 1 cut(s) 885
BsmI GAATGC 1 cut(s) 1066
BsoBI CYCGRG 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 248, 313
Bsp13I TCCGGA 1 cut(s) 438
Bsp143I GATC 6 cut(s) 102, 217, 330, 946, 964, 1165
Bsp1720I GCTNAGC 1 cut(s) 453
BspACI CCGC 4 cut(s) 110, 186, 364, 753
BspCNI CTCAG 3 cut(s) 51, 445, 726
BspDI ATCGAT 1 cut(s) 591
BspEI TCCGGA 1 cut(s) 438
BspFNI CGCG 2 cut(s) 110, 186
BspLI GGNNCC 6 cut(s) 312, 851, 901, 972, 1015, 1110
BspMI ACCTGC 2 cut(s) 294, 571
BspT104I TTCGAA 1 cut(s) 51
BspT107I GGYRCC 2 cut(s) 310, 1108
BsrDI GCAATG 1 cut(s) 640
BsrI ACTGG 2 cut(s) 983, 1190
BssECI CCNNGG 4 cut(s) 686, 868, 1145, 1146
BssMI GATC 6 cut(s) 102, 217, 330, 946, 964, 1165
BssNAI GTATAC 1 cut(s) 464
BssNI GRCGYC 1 cut(s) 205
Bst1107I GTATAC 1 cut(s) 464
Bst2UI CCWGG 4 cut(s) 46, 209, 870, 1147
Bst4CI ACNGT 4 cut(s) 100, 406, 412, 499
BstACI GRCGYC 1 cut(s) 205
BstAPI GCANNNNNTGC 1 cut(s) 827
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 832
BstDEI CTNAG 4 cut(s) 59, 315, 453, 734
BstF5I GGATG 2 cut(s) 328, 355
BstFNI CGCG 2 cut(s) 110, 186
BstKTI GATC 6 cut(s) 105, 220, 333, 949, 967, 1168
BstMAI GTCTC 4 cut(s) 548, 737, 810, 843
BstMBI GATC 6 cut(s) 102, 217, 330, 946, 964, 1165
BstMCI CGRYCG 1 cut(s) 368
BstMWI GCNNNNNNNGC 5 cut(s) 827, 1040, 1049, 1061, 1070
BstNI CCWGG 4 cut(s) 46, 209, 870, 1147
BstSCI CCNGG 5 cut(s) 44, 207, 868, 901, 1145
BstSLI GKGCMC 1 cut(s) 313
BstUI CGCG 2 cut(s) 110, 186
BstV1I GCAGC 6 cut(s) 689, 1039, 1043, 1060, 1082, 1109
BstV2I GAAGAC 2 cut(s) 39, 217
BstX2I RGATCY 1 cut(s) 964
BstYI RGATCY 1 cut(s) 964
BstZ17I GTATAC 1 cut(s) 464
Bsu15I ATCGAT 1 cut(s) 591
BsuTUI ATCGAT 1 cut(s) 591
BtrI CACGTC 2 cut(s) 163, 911
BtsCI GGATG 2 cut(s) 328, 355
BtsIMutI CAGTG 3 cut(s) 105, 417, 1197
BveI ACCTGC 2 cut(s) 294, 571
Cac8I GCNNGC 1 cut(s) 832
CaiI CAGNNNCTG 2 cut(s) 153, 1037
Cfr13I GGNCC 4 cut(s) 142, 433, 899, 971
ClaI ATCGAT 1 cut(s) 591
CseI GACGC 3 cut(s) 213, 827, 1032
Csp6I GTAC 5 cut(s) 175, 292, 299, 1109, 1182
CviAII CATG 2 cut(s) 26, 892
CviQI GTAC 5 cut(s) 175, 292, 299, 1109, 1182
DdeI CTNAG 4 cut(s) 59, 315, 453, 734
DpnI GATC 6 cut(s) 104, 219, 332, 948, 966, 1167
DpnII GATC 6 cut(s) 102, 217, 330, 946, 964, 1165
DraIII CACNNNGTG 1 cut(s) 163
DriI GACNNNNNGTC 1 cut(s) 40
Eam1105I GACNNNNNGTC 1 cut(s) 40
Ecl136II GAGCTC 1 cut(s) 246
Eco24I GRGCYC 1 cut(s) 248
Eco47I GGWCC 4 cut(s) 142, 433, 899, 971
Eco53kI GAGCTC 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 79
EcoICRI GAGCTC 1 cut(s) 246
EcoO109I RGGNCCY 3 cut(s) 433, 899, 971
EcoRII CCWGG 4 cut(s) 44, 207, 868, 1145
EcoT22I ATGCAT 1 cut(s) 508
EcoT38I GRGCYC 1 cut(s) 248
Esp3I CGTCTC 1 cut(s) 810
FaeI CATG 2 cut(s) 29, 895
FaqI GGGAC 1 cut(s) 885
FatI CATG 2 cut(s) 25, 891
FauNDI CATATG 2 cut(s) 823, 1162
FblI GTMKAC 1 cut(s) 463
Fnu4HI GCNGC 6 cut(s) 678, 1032, 1053, 1071, 1074, 1123
FokI GGATG 2 cut(s) 335, 362
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 6 cut(s) 678, 1032, 1053, 1071, 1074, 1123
FspBI CTAG 2 cut(s) 717, 936
GluI GCNGC 6 cut(s) 678, 1032, 1053, 1071, 1074, 1123
GsuI CTGGAG 1 cut(s) 1173
HapII CCGG 2 cut(s) 439, 903
HgaI GACGC 3 cut(s) 213, 827, 1032
Hin1I GRCGYC 1 cut(s) 205
Hin1II CATG 2 cut(s) 29, 895
HinfI GANTC 9 cut(s) 150, 194, 261, 442, 536, 602, 698, 778, 792
HpaII CCGG 2 cut(s) 439, 903
HphI GGTGA 2 cut(s) 79, 972
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 5 cut(s) 23, 130, 236, 687, 735
Hpy188III TCNNGA 8 cut(s) 198, 431, 439, 572, 625, 789, 815, 840
Hpy8I GTNNAC 1 cut(s) 464
Hpy99I CGWCG 2 cut(s) 110, 164
HpyAV CCTTC 2 cut(s) 272, 863
HpyCH4III ACNGT 4 cut(s) 100, 406, 412, 499
HpyCH4IV ACGT 3 cut(s) 162, 910, 1196
HpyCH4V TGCA 8 cut(s) 423, 506, 587, 665, 711, 944, 1070, 1160
HpyF10VI GCNNNNNNNGC 5 cut(s) 827, 1040, 1049, 1061, 1070
HpyF3I CTNAG 4 cut(s) 59, 315, 453, 734
HpySE526I ACGT 3 cut(s) 162, 910, 1196
Hsp92I GRCGYC 1 cut(s) 205
Hsp92II CATG 2 cut(s) 29, 895
Kpn2I TCCGGA 1 cut(s) 438
KpnI GGTACC 1 cut(s) 1112
Kzo9I GATC 6 cut(s) 102, 217, 330, 946, 964, 1165
LmnI GCTCC 1 cut(s) 1019
Lsp1109I GCAGC 6 cut(s) 689, 1039, 1043, 1060, 1082, 1109
LweI GCATC 3 cut(s) 515, 604, 817
MaeI CTAG 2 cut(s) 717, 936
MaeII ACGT 3 cut(s) 162, 910, 1196
MaeIII GTNAC 2 cut(s) 406, 1192
MalI GATC 6 cut(s) 104, 219, 332, 948, 966, 1167
MboI GATC 6 cut(s) 102, 217, 330, 946, 964, 1165
MboII GAAGA 7 cut(s) 44, 82, 217, 220, 1069, 1071, 1160
MflI RGATCY 1 cut(s) 964
MhlI GDGCHC 2 cut(s) 248, 313
MluCI AATT 6 cut(s) 53, 137, 239, 523, 654, 1008
MlyI GAGTC 4 cut(s) 530, 596, 707, 772
MmeI TCCRAC 3 cut(s) 147, 300, 513
Mph1103I ATGCAT 1 cut(s) 508
MroI TCCGGA 1 cut(s) 438
MseI TTAA 2 cut(s) 1127, 1176
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 2 cut(s) 439, 903
MspR9I CCNGG 5 cut(s) 46, 209, 870, 903, 1147
Mva1269I GAATGC 1 cut(s) 1066
MvaI CCWGG 4 cut(s) 46, 209, 870, 1147
MvnI CGCG 2 cut(s) 110, 186
MwoI GCNNNNNNNGC 5 cut(s) 827, 1040, 1049, 1061, 1070
NciI CCSGG 1 cut(s) 903
NdeI CATATG 2 cut(s) 823, 1162
NdeII GATC 6 cut(s) 102, 217, 330, 946, 964, 1165
NlaIII CATG 2 cut(s) 29, 895
NlaIV GGNNCC 6 cut(s) 312, 851, 901, 972, 1015, 1110
NmuCI GTSAC 1 cut(s) 1192
NsiI ATGCAT 1 cut(s) 508
NspV TTCGAA 1 cut(s) 51
PaeR7I CTCGAG 1 cut(s) 79
PaqCI CACCTGC 2 cut(s) 294, 571
PasI CCCWGGG 1 cut(s) 1146
PcsI WCGNNNNNNNCGW 5 cut(s) 111, 126, 219, 228, 597
PctI GAATGC 1 cut(s) 1066
PfeI GAWTC 5 cut(s) 150, 194, 261, 442, 792
Pfl23II CGTACG 1 cut(s) 298
PfoI TCCNGGA 1 cut(s) 901
PkrI GCNGC 6 cut(s) 679, 1033, 1054, 1072, 1075, 1124
PleI GAGTC 4 cut(s) 530, 596, 706, 772
PpsI GAGTC 4 cut(s) 530, 596, 706, 772
PpuMI RGGWCCY 3 cut(s) 433, 899, 971
Psp124BI GAGCTC 1 cut(s) 248
Psp5II RGGWCCY 3 cut(s) 433, 899, 971
Psp6I CCWGG 4 cut(s) 44, 207, 868, 1145
PspGI CCWGG 4 cut(s) 44, 207, 868, 1145
PspLI CGTACG 1 cut(s) 298
PspN4I GGNNCC 6 cut(s) 312, 851, 901, 972, 1015, 1110
PspPI GGNCC 4 cut(s) 142, 433, 899, 971
PspPPI RGGWCCY 3 cut(s) 433, 899, 971
PspXI VCTCGAGB 1 cut(s) 79
PstNI CAGNNNCTG 2 cut(s) 153, 1037
PsuI RGATCY 1 cut(s) 964
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 5 cut(s) 176, 293, 300, 1110, 1183
RsaNI GTAC 5 cut(s) 175, 292, 299, 1109, 1182
SacI GAGCTC 1 cut(s) 248
SaqAI TTAA 2 cut(s) 1127, 1176
SatI GCNGC 6 cut(s) 678, 1032, 1053, 1071, 1074, 1123
Sau3AI GATC 6 cut(s) 102, 217, 330, 946, 964, 1165
Sau96I GGNCC 4 cut(s) 142, 433, 899, 971
SchI GAGTC 4 cut(s) 530, 596, 707, 772
ScrFI CCNGG 5 cut(s) 46, 209, 870, 903, 1147
SduI GDGCHC 2 cut(s) 248, 313
SfaNI GCATC 3 cut(s) 515, 604, 817
Sfr274I CTCGAG 1 cut(s) 79
SfuI TTCGAA 1 cut(s) 51
SinI GGWCC 4 cut(s) 142, 433, 899, 971
SlaI CTCGAG 1 cut(s) 79
SmlI CTYRAG 1 cut(s) 79
SmoI CTYRAG 1 cut(s) 79
Sse9I AATT 6 cut(s) 53, 137, 239, 523, 654, 1008
SsiI CCGC 4 cut(s) 110, 186, 364, 753
SspMI CTAG 2 cut(s) 717, 936
SstI GAGCTC 1 cut(s) 248
StyD4I CCNGG 5 cut(s) 44, 207, 868, 901, 1145
TaaI ACNGT 4 cut(s) 100, 406, 412, 499
TaiI ACGT 3 cut(s) 165, 913, 1199
TaqI TCGA 8 cut(s) 12, 51, 80, 135, 591, 600, 814, 841
TasI AATT 6 cut(s) 53, 137, 239, 523, 654, 1008
TatI WGTACW 1 cut(s) 174
TfiI GAWTC 5 cut(s) 150, 194, 261, 442, 792
Tru1I TTAA 2 cut(s) 1127, 1176
Tru9I TTAA 2 cut(s) 1127, 1176
TscAI CASTG 3 cut(s) 105, 417, 1197
TseFI GTSAC 1 cut(s) 1192
TseI GCWGC 6 cut(s) 677, 1031, 1052, 1070, 1073, 1122
Tsp45I GTSAC 1 cut(s) 1192
TspDTI ATGAA 3 cut(s) 776, 908, 1128
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 3 cut(s) 105, 417, 1197
VpaK11BI GGWCC 4 cut(s) 142, 433, 899, 971
XhoI CTCGAG 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 463
XspI CTAG 2 cut(s) 717, 936
Zsp2I ATGCAT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.