pycom08g08320

Protein TIC 22, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012495
Physical Location & Seq
Reverse (-)
6683444 .. 6686231
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 972 bp
ATGGAATGGGCCACCGAACTGTATGGGCTGGATTGTATTGGGCAACTAATTACAATTCAAACTGGGTTGCAATACTTATTTATCGAAACAACCATTCTGCGCTACTCGTCAGTCGTCACTCGCACTGAAACGATGGAGTCCCCAAAGCCCTACCCGCGCCCAAACCCTCTGCTATCGCTCTCCAGCTTCATACACCAGCACTGCCTCCGCCTCGGCGCCGAGCTCTCCACCCGTCTCGAAGATACCAAGCGGCTCGCCGGAACCATAGCAGGCAACTTCGCGCCGGCGGCGAGCAGGCGCATGCGTTCGGCGCCGGTTTCTCCTCCCTTCGCGTGGCTGGCTCAAAGAAATCACGCTCTGGCCGCTACCCTCAGCTCTGACCACGTGGCGAAGAGCCTGGCCGGGACGGCCGTATATACGGTTAGCAATTCGAACAATGAGTTCGTGCTCATCTCTGATCCCAATGAGGCCAAGTCCATTGGATTGCTTTGCTTTCGGCACGAAGATGCCGAAGCATTCCTCGCTCAAGTTCGATCGAGGAGAGAATTACGAAGTGCGGCTAAAGTTGTCCCGATTACTCTTGACCAGGTGTACATGCTGAAGGTTGAAGGAATTGCATTCCGGTTTCTGCCTGACCCAGTTCAAATAAGGAATGCACTTGAGCTCAAATCCGCTGACAGGAGTGCATTTGATGGAGTTCCTGTTTTTCAGTCCGACCTTCTGGTTATGAAGAAGAAAAACAAGCGTTACTGCCCAATATATTTCACCAAGGAAGATATTGAAAAAGAATTGTCAAAGGTATCCAGAGCATCCAGAGGACCAGGCGTTTCTCAACATATAATGGTTGGCAGTTTGGAAGATGTTTTGAGAAAAATGGAGTCAAGTGAAAAGAATTCAGGCTGGGAAGACCTGATTTTCATTCCACCAGGAAAAAGCTACTCACAGCACATTCAAGATGTGGTGAAACCCTGA

Protein Analysis

324

Amino Acids

36.08

Weight (kDa)

8.94

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 57 - 319 2.2e-110 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33350 AT4G33350
fragaria_vesca FvH4_2g34490
malus_domestica MD08G1102400.v1.1 MD15G1083800.v1.1
prunus_persica Prupe.1G436300_v2.0.a1
pyrus_communis pycom08g08320 pycom15g07850 pycom15g07890
rosa_chinensis RchiOBHm_Chr6g0307851
rosa_laevigata RLG00000010693
rosa_multiflora Rmu_sc0003252.1_g000014
rosa_roxburghii Rroxscaffold_7G00160820
rosa_rugosa Rorug06G0361400
rosa_samantha Rh6AG470200 Rh6BG481200 Rh6CG486500 Rh6DG472700
rosa_wichuraiana Rw6G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 215, 310
AccII CGCG 3 cut(s) 157, 281, 332
AciI CCGC 7 cut(s) 155, 208, 250, 287, 363, 557, 672
AclWI GGATC 1 cut(s) 452
AcoI YGGCCR 3 cut(s) 360, 399, 408
AcsI RAATTY 1 cut(s) 892
AcuI CTGAAG 1 cut(s) 620
AcvI CACGTG 1 cut(s) 385
AcyI GRCGYC 2 cut(s) 216, 311
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 2 cut(s) 333, 678
AgsI TTSAA 5 cut(s) 59, 608, 644, 782, 953
AjnI CCWGG 4 cut(s) 396, 585, 820, 925
AluBI AGCT 5 cut(s) 186, 223, 375, 664, 936
AluI AGCT 5 cut(s) 186, 223, 375, 664, 936
Alw21I GWGCWC 3 cut(s) 225, 450, 666
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 452
AoxI GGCC 5 cut(s) 9, 360, 399, 408, 468
ApoI RAATTY 1 cut(s) 892
AspLEI GCGC 6 cut(s) 102, 159, 218, 283, 300, 313
AspS9I GGNCC 2 cut(s) 9, 818
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 1 cut(s) 757
AsuII TTCGAA 1 cut(s) 431
AvaII GGWCC 1 cut(s) 818
BanI GGYRCC 2 cut(s) 215, 310
BanII GRGCYC 2 cut(s) 225, 666
BbrPI CACGTG 1 cut(s) 385
BbsI GAAGAC 1 cut(s) 912
Bbv12I GWGCWC 3 cut(s) 225, 450, 666
BbvCI CCTCAGC 1 cut(s) 371
BccI CCATC 2 cut(s) 127, 686
BceAI ACGGC 2 cut(s) 395, 423
BciT130I CCWGG 4 cut(s) 398, 587, 822, 927
BciVI GTATCC 1 cut(s) 811
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 239
BfoI RGCGCY 2 cut(s) 219, 314
BfuI GTATCC 1 cut(s) 811
BglI GCCNNNNNGGC 1 cut(s) 407
BisI GCNGC 4 cut(s) 251, 288, 363, 558
BlsI GCNGC 4 cut(s) 252, 289, 364, 559
Bme1390I CCNGG 5 cut(s) 398, 403, 587, 822, 927
Bme18I GGWCC 1 cut(s) 818
BmgT120I GGNCC 2 cut(s) 9, 818
BmiI GGNNCC 3 cut(s) 217, 262, 312
BmrFI CCNGG 5 cut(s) 398, 403, 587, 822, 927
BmrI ACTGGG 2 cut(s) 72, 632
BmsI GCATC 2 cut(s) 496, 818
BmuI ACTGGG 2 cut(s) 72, 632
BpiI GAAGAC 1 cut(s) 912
BpmI CTGGAG 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 371
Bpu14I TTCGAA 1 cut(s) 431
BpuEI CTTGAG 2 cut(s) 510, 680
BpuMI CCSGG 1 cut(s) 403
BsaAI YACGTR 1 cut(s) 385
BsaHI GRCGYC 2 cut(s) 216, 311
BsaJI CCNNGG 2 cut(s) 211, 768
BsaWI WCCGGW 1 cut(s) 621
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bsc4I CCNNNNNNNGG 2 cut(s) 333, 678
Bse118I RCCGGY 2 cut(s) 283, 313
Bse1I ACTGG 2 cut(s) 67, 638
BseBI CCWGG 4 cut(s) 398, 587, 822, 927
BseDI CCNNGG 2 cut(s) 211, 768
BseGI GGATG 1 cut(s) 809
BseLI CCNNNNNNNGG 2 cut(s) 333, 678
BseMII CTCAG 1 cut(s) 385
BseNI ACTGG 2 cut(s) 67, 638
BseRI GAGGAG 2 cut(s) 312, 553
BseX3I CGGCCG 1 cut(s) 408
BseYI CCCAGC 1 cut(s) 900
Bsh1236I CGCG 3 cut(s) 157, 281, 332
Bsh1285I CGRYCG 2 cut(s) 411, 536
BshFI GGCC 5 cut(s) 11, 362, 401, 410, 470
BshNI GGYRCC 2 cut(s) 215, 310
BsiEI CGRYCG 2 cut(s) 411, 536
BsiHKAI GWGCWC 3 cut(s) 225, 450, 666
BsiSI CCGG 5 cut(s) 258, 284, 314, 402, 622
BslFI GGGAC 3 cut(s) 124, 418, 554
BslI CCNNNNNNNGG 2 cut(s) 333, 678
BsmAI GTCTC 1 cut(s) 239
BsmBI CGTCTC 1 cut(s) 239
BsmFI GGGAC 3 cut(s) 124, 418, 554
BsmI GAATGC 3 cut(s) 515, 617, 658
BsnI GGCC 5 cut(s) 11, 362, 401, 410, 470
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 3 cut(s) 225, 450, 666
Bsp1407I TGTACA 1 cut(s) 591
Bsp143I GATC 2 cut(s) 457, 533
BspACI CCGC 7 cut(s) 155, 208, 250, 287, 363, 557, 672
BspANI GGCC 5 cut(s) 11, 362, 401, 410, 470
BspCNI CTCAG 1 cut(s) 384
BspFNI CGCG 3 cut(s) 157, 281, 332
BspLI GGNNCC 3 cut(s) 217, 262, 312
BspPI GGATC 1 cut(s) 452
BspQI GCTCTTC 1 cut(s) 386
BspT104I TTCGAA 1 cut(s) 431
BspT107I GGYRCC 2 cut(s) 215, 310
BsrFI RCCGGY 2 cut(s) 283, 313
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 2 cut(s) 67, 638
BssAI RCCGGY 2 cut(s) 283, 313
BssECI CCNNGG 2 cut(s) 211, 768
BssMI GATC 2 cut(s) 457, 533
BssNI GRCGYC 2 cut(s) 216, 311
BssT1I CCWWGG 1 cut(s) 768
Bst2UI CCWGG 4 cut(s) 398, 587, 822, 927
Bst4CI ACNGT 2 cut(s) 21, 421
Bst6I CTCTTC 1 cut(s) 386
BstACI GRCGYC 2 cut(s) 216, 311
BstAUI TGTACA 1 cut(s) 591
BstBAI YACGTR 1 cut(s) 385
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 7 cut(s) 255, 271, 285, 292, 296, 302, 339
BstDEI CTNAG 1 cut(s) 371
BstF5I GGATG 1 cut(s) 809
BstFNI CGCG 3 cut(s) 157, 281, 332
BstH2I RGCGCY 2 cut(s) 219, 314
BstHHI GCGC 6 cut(s) 102, 159, 218, 283, 300, 313
BstKTI GATC 2 cut(s) 460, 536
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 2 cut(s) 457, 533
BstMCI CGRYCG 2 cut(s) 411, 536
BstMWI GCNNNNNNNGC 7 cut(s) 154, 287, 310, 338, 362, 407, 521
BstNI CCWGG 4 cut(s) 398, 587, 822, 927
BstNSI RCATGY 2 cut(s) 304, 598
BstSCI CCNGG 5 cut(s) 396, 401, 585, 820, 925
BstUI CGCG 3 cut(s) 157, 281, 332
BstV2I GAAGAC 1 cut(s) 912
BstZI CGGCCG 1 cut(s) 408
BsuI GTATCC 1 cut(s) 811
BsuRI GGCC 5 cut(s) 11, 362, 401, 410, 470
BtsCI GGATG 1 cut(s) 809
BtsI GCAGTG 1 cut(s) 199
BtsIMutI CAGTG 2 cut(s) 123, 199
Cac8I GCNNGC 7 cut(s) 255, 271, 285, 292, 296, 302, 339
CfoI GCGC 6 cut(s) 102, 159, 218, 283, 300, 313
Cfr10I RCCGGY 2 cut(s) 283, 313
Cfr13I GGNCC 2 cut(s) 9, 818
CsiI ACCWGGT 1 cut(s) 585
Csp6I GTAC 1 cut(s) 592
CviAII CATG 2 cut(s) 301, 595
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 1 cut(s) 371
DinI GGCGCC 2 cut(s) 217, 312
DpnI GATC 2 cut(s) 459, 535
DpnII GATC 2 cut(s) 457, 533
EaeI YGGCCR 3 cut(s) 360, 399, 408
EagI CGGCCG 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
EciI GGCGGA 1 cut(s) 197
Ecl136II GAGCTC 2 cut(s) 223, 664
EclXI CGGCCG 1 cut(s) 408
Eco130I CCWWGG 1 cut(s) 768
Eco24I GRGCYC 2 cut(s) 225, 666
Eco47I GGWCC 1 cut(s) 818
Eco52I CGGCCG 1 cut(s) 408
Eco53kI GAGCTC 2 cut(s) 223, 664
Eco57I CTGAAG 1 cut(s) 620
Eco72I CACGTG 1 cut(s) 385
EcoICRI GAGCTC 2 cut(s) 223, 664
EcoRI GAATTC 1 cut(s) 892
EcoRII CCWGG 4 cut(s) 396, 585, 820, 925
EcoT14I CCWWGG 1 cut(s) 768
EcoT38I GRGCYC 2 cut(s) 225, 666
EgeI GGCGCC 2 cut(s) 217, 312
EheI GGCGCC 2 cut(s) 217, 312
ErhI CCWWGG 1 cut(s) 768
Esp3I CGTCTC 1 cut(s) 239
FaeI CATG 2 cut(s) 304, 598
FaqI GGGAC 3 cut(s) 124, 418, 554
FatI CATG 2 cut(s) 300, 594
FauI CCCGC 1 cut(s) 162
Fnu4HI GCNGC 4 cut(s) 251, 288, 363, 558
FokI GGATG 1 cut(s) 796
FriOI GRGCYC 2 cut(s) 225, 666
Fsp4HI GCNGC 4 cut(s) 251, 288, 363, 558
GlaI GCGC 6 cut(s) 101, 158, 217, 282, 299, 312
GluI GCNGC 4 cut(s) 251, 288, 363, 558
GsaI CCCAGC 1 cut(s) 904
GsuI CTGGAG 1 cut(s) 166
HaeII RGCGCY 2 cut(s) 219, 314
HaeIII GGCC 5 cut(s) 11, 362, 401, 410, 470
HapII CCGG 5 cut(s) 258, 284, 314, 402, 622
HhaI GCGC 6 cut(s) 102, 159, 218, 283, 300, 313
Hin1I GRCGYC 2 cut(s) 216, 311
Hin1II CATG 2 cut(s) 304, 598
Hin6I GCGC 6 cut(s) 100, 157, 216, 281, 298, 311
HinP1I GCGC 6 cut(s) 100, 157, 216, 281, 298, 311
HinfI GANTC 2 cut(s) 137, 878
HpaII CCGG 5 cut(s) 258, 284, 314, 402, 622
HphI GGTGA 1 cut(s) 757
Hpy166II GTNNAC 1 cut(s) 592
Hpy188I TCNGA 3 cut(s) 379, 457, 715
Hpy188III TCNNGA 6 cut(s) 236, 571, 581, 804, 813, 953
Hpy8I GTNNAC 1 cut(s) 592
HpyAV CCTTC 4 cut(s) 337, 595, 602, 728
HpyCH4III ACNGT 2 cut(s) 21, 421
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 4 cut(s) 70, 617, 656, 686
HpyF10VI GCNNNNNNNGC 7 cut(s) 154, 287, 310, 338, 362, 407, 521
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 1 cut(s) 384
Hsp92I GRCGYC 2 cut(s) 216, 311
Hsp92II CATG 2 cut(s) 304, 598
HspAI GCGC 6 cut(s) 100, 157, 216, 281, 298, 311
KasI GGCGCC 2 cut(s) 215, 310
KroI GCCGGC 1 cut(s) 283
KroNI GCCGGC 1 cut(s) 285
Kzo9I GATC 2 cut(s) 457, 533
LguI GCTCTTC 1 cut(s) 386
LweI GCATC 2 cut(s) 496, 818
MabI ACCWGGT 1 cut(s) 585
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 2 cut(s) 115, 746
MalI GATC 2 cut(s) 459, 535
MboI GATC 2 cut(s) 457, 533
MboII GAAGA 8 cut(s) 251, 403, 515, 742, 745, 785, 869, 917
MhlI GDGCHC 3 cut(s) 225, 450, 666
MluCI AATT 7 cut(s) 48, 54, 427, 545, 612, 788, 892
Mly113I GGCGCC 2 cut(s) 216, 311
MlyI GAGTC 2 cut(s) 146, 887
MmeI TCCRAC 1 cut(s) 738
MnlI CCTC 9 cut(s) 177, 215, 221, 333, 380, 460, 530, 531, 809
MreI CGCCGGCG 1 cut(s) 283
MroNI GCCGGC 1 cut(s) 283
MslI CAYNNNNRTG 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 674
MspI CCGG 5 cut(s) 258, 284, 314, 402, 622
MspR9I CCNGG 5 cut(s) 398, 403, 587, 822, 927
Mva1269I GAATGC 3 cut(s) 515, 617, 658
MvaI CCWGG 4 cut(s) 398, 587, 822, 927
MvnI CGCG 3 cut(s) 157, 281, 332
MwoI GCNNNNNNNGC 7 cut(s) 154, 287, 310, 338, 362, 407, 521
NaeI GCCGGC 1 cut(s) 285
NarI GGCGCC 2 cut(s) 216, 311
NciI CCSGG 1 cut(s) 403
NdeII GATC 2 cut(s) 457, 533
NgoMIV GCCGGC 1 cut(s) 283
NlaIII CATG 2 cut(s) 304, 598
NlaIV GGNNCC 3 cut(s) 217, 262, 312
NmeAIII GCCGAG 2 cut(s) 192, 244
NmuCI GTSAC 1 cut(s) 115
NspI RCATGY 2 cut(s) 304, 598
NspV TTCGAA 1 cut(s) 431
PaeI GCATGC 1 cut(s) 304
PciSI GCTCTTC 1 cut(s) 386
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PctI GAATGC 3 cut(s) 515, 617, 658
PdiI GCCGGC 1 cut(s) 285
PkrI GCNGC 4 cut(s) 252, 289, 364, 559
Ple19I CGATCG 1 cut(s) 536
PleI GAGTC 2 cut(s) 145, 886
PluTI GGCGCC 2 cut(s) 219, 314
PmaCI CACGTG 1 cut(s) 385
PmlI CACGTG 1 cut(s) 385
PpsI GAGTC 2 cut(s) 145, 886
Ppu21I YACGTR 1 cut(s) 385
Psp124BI GAGCTC 2 cut(s) 225, 666
Psp6I CCWGG 4 cut(s) 396, 585, 820, 925
PspCI CACGTG 1 cut(s) 385
PspFI CCCAGC 1 cut(s) 900
PspGI CCWGG 4 cut(s) 396, 585, 820, 925
PspN4I GGNNCC 3 cut(s) 217, 262, 312
PspPI GGNCC 2 cut(s) 9, 818
PvuI CGATCG 1 cut(s) 536
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
RseI CAYNNNNRTG 1 cut(s) 504
SacI GAGCTC 2 cut(s) 225, 666
SapI GCTCTTC 1 cut(s) 386
SatI GCNGC 4 cut(s) 251, 288, 363, 558
Sau3AI GATC 2 cut(s) 457, 533
Sau96I GGNCC 2 cut(s) 9, 818
SchI GAGTC 2 cut(s) 146, 887
ScrFI CCNGG 5 cut(s) 398, 403, 587, 822, 927
SduI GDGCHC 3 cut(s) 225, 450, 666
SexAI ACCWGGT 1 cut(s) 585
SfaNI GCATC 2 cut(s) 496, 818
SfiI GGCCNNNNNGGCC 1 cut(s) 407
SfoI GGCGCC 2 cut(s) 217, 312
SfuI TTCGAA 1 cut(s) 431
SgrAI CRCCGGYG 1 cut(s) 283
SinI GGWCC 1 cut(s) 818
SmiMI CAYNNNNRTG 1 cut(s) 504
SmlI CTYRAG 2 cut(s) 525, 659
SmoI CTYRAG 2 cut(s) 525, 659
SphI GCATGC 1 cut(s) 304
Sse9I AATT 7 cut(s) 48, 54, 427, 545, 612, 788, 892
SsiI CCGC 7 cut(s) 155, 208, 250, 287, 363, 557, 672
SspDI GGCGCC 2 cut(s) 215, 310
SstI GAGCTC 2 cut(s) 225, 666
StyD4I CCNGG 5 cut(s) 396, 401, 585, 820, 925
StyI CCWWGG 1 cut(s) 768
TaaI ACNGT 2 cut(s) 21, 421
TaiI ACGT 1 cut(s) 387
TaqI TCGA 5 cut(s) 84, 237, 431, 532, 536
TasI AATT 7 cut(s) 48, 54, 427, 545, 612, 788, 892
TatI WGTACW 1 cut(s) 591
TauI GCSGC 4 cut(s) 253, 290, 365, 560
TscAI CASTG 2 cut(s) 130, 206
TseFI GTSAC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 3 cut(s) 178, 743, 907
TspRI CASTG 2 cut(s) 130, 206
VpaK11BI GGWCC 1 cut(s) 818
XapI RAATTY 1 cut(s) 892
XceI RCATGY 2 cut(s) 304, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.