Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0001943

Physical Location & Seq

Genomic Coordinates Reverse (-)

5728972 .. 5736141

Gene Structure 7170 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3234 bp

ATGTCCACACGCAAAGATGTTTGTATGAAAAGGACAAATGGTCTCTCCTCTTCCACTCTCCACGTTCCTCTCTCTCTCTCTCTCTCTCTCTCTCATTTCTTCACTTCTCTGAGCTTCTTCAGGAGCTGCAACTCAGAGTTTGAGCCAAATATGGAAAGCCATCAGCTTTCATTCCCAACGTTGCTTCTTGCATGTCTCATCTGTTCCTCTACATTTTTGTTCTTTGCAACTTTTGGTCAGTCAGCCACCACCGCCAAGCTTCACACTCAAGACGTGAATGTGTTGAAAGTGATAGGGAAAAAGTTGGGGAAGAAAGACTGGGATTTTGGAAAAGATCCATGTACAGGAGAAGGGAATTGGACGATCCCGATTAGTAGGGGGAAGAAAGGCTTTGACAGTGCTGTCATATGTGATTGCACCTTCAACTACAATTCCTCTTGCCGCGTCATCAGAATATTTCTAAAGGCCCAGAATCTTTCAGGGACCGTGCCACCAGAGTTTTCGAAGCTTCCTTACCTCCAAGAATTGGACTTGAGTCGAAATGTCCTCAACGGTACTATACCTTCCCAATGGGCTACCATGCGCTTGCACACGCTGTCTTTTATGGGAAACAGATTGTCAGGACCATTTCCAAAAGTTCTTACCAATATCACCACGCTCAGAAACTTGAGCATTGAAGGAAACCTTTTCTCAGGACCCATTCCGCGGGAAATTGGAAAGTTGATCAAACTAGAAAAGCTCATTCTGTCTTCAAATGCCTTCACCGGAGAATTGCCATCAACACTGGTAAAGCTGACGAACTTGTCTGATATGAGGATAAGTGACAATAATTTCTCTGGAAAGATACCTGACTTCATTGGAAATTGGACGCGGGTTTCAAAACTGCTTATCGAGGGTAGCTCTCTTGAGGGGCCTATACCTTCTACCATATCCGCCTTAACAAGTTTAACTCATCTGAGGATAACCGACTTAAAAGGAAACAGGTCTCCTTTCCCGCCATTGAGTAATTTGGAATCCTTGAAAATATTGATACTGAGGAATTGCTTGATACACGGAGAAATCCCAGATTACATTGGGGATATGGAAAACTTGAAAAACTTAGACCTCAGCTACAATGAGTTGACGGGTGAAATACCTGCATCTTTTGTCCAACTGGAAAAAATTGAAATTATGTATTTGACAGGAAACCAGCTCACTGGAACCATACCGGGATGGATCCCGTCAAGTAACAATATTGTGGATATTTCTTACAACAACTTCACCTGGGAAGGTTCAAGTCCTAATGAATGCTCTAGGGGAAGTGTCAACTTAGTGGAGAGCTACTCCTCGTCAGCCGATAAATTAAAAATACAACCGTGCATGAAAAGAAATTTTCCTTGTCATACTCCGAAAGATCAGCGTAAACATTCACTGTATATTAACTGTGGCGGAAAGGAAGTAATAATCGGTGGCAAGAAGTATGAAGCTGATAGAGAACCAAAGGGTGCATCAATGTATTACATGGGTCAGAACTGGGCTTTCAGCAGCACAGGAAACTTCATGGACAACGATGCTGATTCAGATACCTACATTGACACCAATACATCTGCACTTACAAAAAATGTCTCTGCGCTTGATTCAGAACTGTACACAACAGCGCGGGCATCTCCCATCTCGCTCACATACTATGGTCTCTGTCTTTTTAAAGGAAACTACACCGTGCGTCTCCACTTTGCTGAAATTGTTTTTACTAATGACAGAACATTTTACAGCCTTGGCAAGCGTGTATTTGATGTCTACATCCAGGATAAGCTGGTCCTGAAAGATTTCGATATAGCAAGTGAAGCTGGGGATGCTGGCAAGCCGATTGTTAAAGCGTTCACTTCAGTTGTTTCGAGTCATACATTAAAGATCCATTTTTACTGGGCTGGAAAAGGGACAACGGGCATCCCAGACAGAGGATTTTATGGTCCTCTCATATCGGCTATATCAGTAGATGCTAATTTTAAACTTCCTTCGGTTGATGGCCACAAGAATCATCTCGTTAAAGTAGTTGGGGGAGTGGCCGGAGCACTAGTACTTCTTCTGCTACTGGTCTTAGGCATCATGAGGAGAAAGGGCTGGTTGGGAGGAAAGATCGCTGCAGATAAAGAATTCAAAGATTTAGATCTTCAAACAGGACTATATACTTTAAGACAGATAAAGGCTGCAACAAAAAACTTTGATGCAGCAAACAAACTCGGGGAGGGCGGCTTTGGTGTGGTCTACAAGGGTGAATTATCAGACGGCACAGTAATTGCGGTGAAGCAGCTGTCCTCAAAATCAAAGCAAGGAAACCGTGAATTTGTGAATGAAATCGGCATGATTTCTGCCCTGCAACATCCAAACCTTGTGAAGCTTTATGGGTGCTGTGTTGAAGGGAACCAGATGCTGTTAATTTACGAGTACATGGAAAATAACTGTGTGTCCCGAAGTGACCCGACTTGCAGACTGGAACTCGACTGGCCTACAAGGAAGAAGATTTGCATTGGTATTGCTAGAGGCTTGGCCTATCTCCACGAAGAATCAAGACTAAGAATCGTGCATCGTGATATCAAGACAAGCAATGTGTTGCTTGATAAGGACTTCAATGCTAAAATTTCAGATTTTGGCTTGGCAAAGCTTAATGAAGACGACAAAACCCACATCAGCACGCGGGTTGCTGGAACTATTGGCTATATGGCTCCCGAGTATGCAATGCGTGGTTACTTGACTGACAAGGCAGATGTTTATAGCTTTGGAGTTGTTGCACTTGAAATCGTTAGTGGAAAGAGCAACACAAACTACAGGCCAAAGGAGGAATTCGTCTACCTTCTTGACTGGGCCTATGTGCTGCAAGAGAGAGGGAGTCTATTGGAGTTGGTTGATCCAGCCTTGGGTTCAGAGTACTCATCAGAAGAGACCATGGTGGTGCTGAATGTTGCTCTCTTGTGCACCAATGCATCTCCCACCCTTAGGCCTACAATGACCCAAGTAGTAAGCATGCTCGAAGGACGAACGGCGGTTCAAGACCTGCTTTCGGATCGATCCGGGTTTTCAGCCATCAATTCCAAGTTCAGGGCCATCAGAAATCACTTCTGGCAACAGCCAAGTTGTACTCAGAGCATGTCAACCAACGGTCCAAAAACTGATACTTCCGGTTCGTACATTGAAACAGAAAAGAGTGCTGATCTCTTGAGAGTTGAATCGTTGACTTCAGTTAAATCTGATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

1078

Amino Acids

119.22

Weight (kDa)

8.35

Isoelectric Point (pI)

29.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	214 - 322	3.7e-09	Leucine-rich repeat region
LRR_14	PF23598	310 - 395	5.3e-07	Leucine-rich repeat region
LRR_8	PF13855	339 - 398	2.2e-09	Leucine rich repeat
Malectin	PF11721	471 - 656	1.2e-40	Malectin domain
Pkinase	PF00069	736 - 1000	8.1e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	737 - 1002	1.8e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0001943)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07650 AT1G07650 AT1G07650 AT1G07650
fragaria_vesca	FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550 FvH4_6g37550
malus_domestica	MD09G1156900.v1.1
prunus_persica	Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1 Prupe.3G168800_v2.0.a1
pyrus_communis	pycom09g07560 pycom11g23700
rosa_chinensis	RchiOBHm_Chr2g0149971 RchiOBHm_Chr2g0150431
rosa_laevigata	RLG00000000535 RLG00000001460 RLG00000011700 RLG00000016818 RLG00000020498 RLG00000030032 RLG00000034736
rosa_multiflora	Rmu_co8438829.1_g000001 Rmu_sc0020041.1_g000001 Rmu_ssc0000480.1_g000017
rosa_roxburghii	Rroxscaffold_2G00097110
rosa_rugosa	Rorug02G0421300
rosa_samantha	Rh2DG501600 Rh4AG126900
rosa_wichuraiana	Rw2G039240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	401, 802
Acc36I	ACCTGC	2 cut(s)	1144, 3039
AccB7I	CCANNNNNTGG	1 cut(s)	526
AccI	GTMKAC	3 cut(s)	1776, 2244, 2826
AccII	CGCG	5 cut(s)	444, 706, 871, 1639, 2674
AclI	AACGTT	1 cut(s)	179
AclWI	GGATC	8 cut(s)	329, 358, 1210, 1223, 1885, 2879, 3039, 3048
AcoI	YGGCCR	2 cut(s)	2005, 2043
AcsI	RAATTY	5 cut(s)	1369, 2132, 2321, 2616, 2819
AcuI	CTGAAG	3 cut(s)	103, 1848, 3198
AfaI	GTAC	8 cut(s)	343, 556, 1628, 2058, 2426, 2906, 3115, 3164
AfiI	CCNNNNNNNGG	8 cut(s)	344, 526, 705, 1937, 2894, 2973, 3037, 3075
AhdI	GACNNNNNGTC	1 cut(s)	39
AhlI	ACTAGT	1 cut(s)	2053
AjiI	CACGTC	1 cut(s)	274
AjnI	CCWGG	2 cut(s)	1262, 1782
Alw21I	GWGCWC	2 cut(s)	2053, 2954
Alw26I	GTCTC	7 cut(s)	47, 200, 990, 1609, 1676, 1709, 2912
Alw44I	GTGCAC	1 cut(s)	2950
AlwI	GGATC	8 cut(s)	329, 358, 1210, 1223, 1885, 2879, 3039, 3048
AlwNI	CAGNNNCTG	2 cut(s)	126, 2410
Ama87I	CYCGRG	2 cut(s)	2219, 2705
ApaLI	GTGCAC	1 cut(s)	2950
ApeKI	GCWGC	7 cut(s)	126, 1524, 2120, 2186, 2207, 2287, 2851
ApoI	RAATTY	5 cut(s)	1369, 2132, 2321, 2616, 2819
ArsI	GACNNNNNNTTYG	2 cut(s)	308, 340
Asp700I	GAANNNNTTC	1 cut(s)	1805
AspLEI	GCGC	3 cut(s)	585, 1612, 1639
AsuC2I	CCSGG	2 cut(s)	1209, 3049
AsuHPI	GGTGA	6 cut(s)	643, 754, 1139, 1252, 2264, 2293
AsuII	TTCGAA	1 cut(s)	503
AvaI	CYCGRG	2 cut(s)	2219, 2705
AvaII	GGWCC	6 cut(s)	483, 623, 695, 1795, 1949, 3137
AxyI	CCTNAGG	1 cut(s)	2972
BaeGI	GKGCMC	1 cut(s)	2954
BalI	TGGCCA	1 cut(s)	2007
BamHI	GGATCC	1 cut(s)	1215
BarI	GAAGNNNNNNTAC	4 cut(s)	497, 529, 547, 579
BbsI	GAAGAC	2 cut(s)	741, 2655
Bbv12I	GWGCWC	2 cut(s)	2053, 2954
BbvCI	CCTCAGC	1 cut(s)	1106
BbvI	GCAGC	7 cut(s)	113, 1536, 2107, 2173, 2219, 2299, 2838
BccI	CCATC	7 cut(s)	168, 784, 1206, 1658, 1997, 3068, 3089
BceAI	ACGGC	2 cut(s)	2281, 3033
BciT130I	CCWGG	2 cut(s)	1264, 1784
BclI	TGATCA	1 cut(s)	723
BcnI	CCSGG	2 cut(s)	1209, 3049
BcoDI	GTCTC	7 cut(s)	47, 200, 990, 1609, 1676, 1709, 2912
BcuI	ACTAGT	1 cut(s)	2053
BfaI	CTAG	4 cut(s)	731, 1293, 2054, 2517
BfmI	CTRYAG	2 cut(s)	2121, 2803
BfuAI	ACCTGC	2 cut(s)	1144, 3039
BglII	AGATCT	1 cut(s)	2146
BisI	GCNGC	9 cut(s)	127, 442, 1525, 2121, 2187, 2208, 2230, 2288, 2852
BlsI	GCNGC	9 cut(s)	128, 443, 1526, 2122, 2188, 2209, 2231, 2289, 2853
BmcAI	AGTACT	2 cut(s)	2058, 2906
Bme1390I	CCNGG	4 cut(s)	1209, 1264, 1784, 3049
Bme18I	GGWCC	6 cut(s)	483, 623, 695, 1795, 1949, 3137
BmeRI	GACNNNNNGTC	1 cut(s)	39
BmeT110I	CYCGRG	2 cut(s)	2219, 2705
BmgBI	CACGTC	1 cut(s)	274
BmiI	GGNNCC	7 cut(s)	484, 697, 912, 1201, 1217, 2402, 2703
BmrFI	CCNGG	4 cut(s)	1209, 1264, 1784, 3049
BmrI	ACTGGG	4 cut(s)	328, 1522, 1912, 2848
BmuI	ACTGGG	4 cut(s)	328, 1522, 1912, 2848
BoxI	GACNNNNGTC	1 cut(s)	534
BpiI	GAAGAC	2 cut(s)	741, 2655
BplI	GAGNNNNNCTC	4 cut(s)	884, 916, 1307, 1339
Bpu10I	CCTNAGC	1 cut(s)	1106
Bpu14I	TTCGAA	1 cut(s)	503
BpuEI	CTTGAG	5 cut(s)	252, 553, 688, 926, 3214
BpuMI	CCSGG	2 cut(s)	1209, 3049
Bsa29I	ATCGAT	1 cut(s)	3043
BsaBI	GATNNNNATC	1 cut(s)	2145
BsaI	GGTCTC	4 cut(s)	47, 990, 1676, 2912
BsaJI	CCNNGG	5 cut(s)	704, 1263, 1753, 2892, 2922
BsaWI	WCCGGW	2 cut(s)	764, 3155
BsaXI	ACNNNNNCTCC	2 cut(s)	2040, 2070
Bsc4I	CCNNNNNNNGG	8 cut(s)	344, 526, 705, 1937, 2894, 2973, 3037, 3075
Bse21I	CCTNAGG	1 cut(s)	2972
Bse3DI	GCAATG	2 cut(s)	2590, 2721
Bse8I	GATNNNNATC	1 cut(s)	2145
BseBI	CCWGG	2 cut(s)	1264, 1784
BseCI	ATCGAT	1 cut(s)	3043
BseDI	CCNNGG	5 cut(s)	704, 1263, 1753, 2892, 2922
BseGI	GGATG	5 cut(s)	1217, 1779, 1837, 1926, 2359
BseJI	GATNNNNATC	1 cut(s)	2145
BseLI	CCNNNNNNNGG	8 cut(s)	344, 526, 705, 1937, 2894, 2973, 3037, 3075
BseMI	GCAATG	2 cut(s)	2590, 2721
BseMII	CTCAG	8 cut(s)	101, 147, 673, 705, 947, 1025, 1120, 3131
BseRI	GAGGAG	3 cut(s)	37, 1315, 2104
BseSI	GKGCMC	1 cut(s)	2954
BseXI	GCAGC	7 cut(s)	113, 1536, 2107, 2173, 2219, 2299, 2838
BseYI	CCCAGC	1 cut(s)	1826
BsgI	GTGCAG	1 cut(s)	1572
Bsh1236I	CGCG	5 cut(s)	444, 706, 871, 1639, 2674
BshVI	ATCGAT	1 cut(s)	3043
BsiHKAI	GWGCWC	2 cut(s)	2053, 2954
BsiHKCI	CYCGRG	2 cut(s)	2219, 2705
BsiSI	CCGG	5 cut(s)	765, 1208, 2046, 3048, 3156
BslFI	GGGAC	3 cut(s)	496, 1930, 2431
BslI	CCNNNNNNNGG	8 cut(s)	344, 526, 705, 1937, 2894, 2973, 3037, 3075
BsmAI	GTCTC	7 cut(s)	47, 200, 990, 1609, 1676, 1709, 2912
BsmBI	CGTCTC	1 cut(s)	1709
BsmFI	GGGAC	3 cut(s)	496, 1930, 2431
BsmI	GAATGC	1 cut(s)	1292
Bso31I	GGTCTC	4 cut(s)	47, 990, 1676, 2912
BsoBI	CYCGRG	2 cut(s)	2219, 2705
Bsp119I	TTCGAA	1 cut(s)	503
Bsp1286I	GDGCHC	2 cut(s)	2053, 2954
Bsp1407I	TGTACA	2 cut(s)	341, 1626
Bsp19I	CCATGG	1 cut(s)	2922
BspCNI	CTCAG	8 cut(s)	102, 146, 672, 704, 948, 1026, 1119, 3130
BspDI	ATCGAT	1 cut(s)	3043
BspFNI	CGCG	5 cut(s)	444, 706, 871, 1639, 2674
BspHI	TCATGA	1 cut(s)	2085
BspLI	GGNNCC	7 cut(s)	484, 697, 912, 1201, 1217, 2402, 2703
BspMAI	CTGCAG	1 cut(s)	2125
BspMI	ACCTGC	2 cut(s)	1144, 3039
BspPI	GGATC	8 cut(s)	329, 358, 1210, 1223, 1885, 2879, 3039, 3048
BspT104I	TTCGAA	1 cut(s)	503
BspTNI	GGTCTC	4 cut(s)	47, 990, 1676, 2912
BsrDI	GCAATG	2 cut(s)	2590, 2721
BsrGI	TGTACA	2 cut(s)	341, 1626
BssECI	CCNNGG	5 cut(s)	704, 1263, 1753, 2892, 2922
BssT1I	CCWWGG	3 cut(s)	1753, 2892, 2922
Bst2UI	CCWGG	2 cut(s)	1264, 1784
Bst6I	CTCTTC	2 cut(s)	55, 2910
BstAUI	TGTACA	2 cut(s)	341, 1626
BstBI	TTCGAA	1 cut(s)	503
BstC8I	GCNNGC	7 cut(s)	587, 1641, 1760, 1837, 1841, 2672, 3002
BstDSI	CCRYGG	2 cut(s)	704, 2922
BstF5I	GGATG	5 cut(s)	1217, 1779, 1837, 1926, 2359
BstFNI	CGCG	5 cut(s)	444, 706, 871, 1639, 2674
BstHHI	GCGC	3 cut(s)	585, 1612, 1639
BstMAI	GTCTC	7 cut(s)	47, 200, 990, 1609, 1676, 1709, 2912
BstMWI	GCNNNNNNNGC	3 cut(s)	251, 1823, 1832
BstNI	CCWGG	2 cut(s)	1264, 1784
BstNSI	RCATGY	3 cut(s)	195, 3004, 3127
BstPAI	GACNNNNGTC	1 cut(s)	534
BstSCI	CCNGG	4 cut(s)	1207, 1262, 1782, 3047
BstSFI	CTRYAG	2 cut(s)	2121, 2803
BstSLI	GKGCMC	1 cut(s)	2954
BstUI	CGCG	5 cut(s)	444, 706, 871, 1639, 2674
BstV1I	GCAGC	7 cut(s)	113, 1536, 2107, 2173, 2219, 2299, 2838
BstV2I	GAAGAC	2 cut(s)	741, 2655
BstX2I	RGATCY	4 cut(s)	334, 1215, 1890, 2146
BstXI	CCANNNNNNTGG	1 cut(s)	1196
BstYI	RGATCY	4 cut(s)	334, 1215, 1890, 2146
Bsu15I	ATCGAT	1 cut(s)	3043
Bsu36I	CCTNAGG	1 cut(s)	2972
BsuTUI	ATCGAT	1 cut(s)	3043
BtgI	CCRYGG	2 cut(s)	704, 2922
BtrI	CACGTC	1 cut(s)	274
BtsCI	GGATG	5 cut(s)	1217, 1779, 1837, 1926, 2359
BtsIMutI	CAGTG	4 cut(s)	403, 782, 1194, 1409
BveI	ACCTGC	2 cut(s)	1144, 3039
Cac8I	GCNNGC	7 cut(s)	587, 1641, 1760, 1837, 1841, 2672, 3002
CaiI	CAGNNNCTG	2 cut(s)	126, 2410
CciI	TCATGA	1 cut(s)	2085
CfoI	GCGC	3 cut(s)	585, 1612, 1639
Cfr42I	CCGCGG	1 cut(s)	707
ClaI	ATCGAT	1 cut(s)	3043
CseI	GACGC	3 cut(s)	433, 877, 1691
Csp6I	GTAC	8 cut(s)	342, 555, 1627, 2057, 2425, 2905, 3114, 3163
CviQI	GTAC	8 cut(s)	342, 555, 1627, 2057, 2425, 2905, 3114, 3163
DraI	TTTAAA	2 cut(s)	1684, 1987
DrdI	GACNNNNNNGTC	2 cut(s)	401, 802
DriI	GACNNNNNGTC	1 cut(s)	39
DseDI	GACNNNNNNGTC	2 cut(s)	401, 802
EaeI	YGGCCR	2 cut(s)	2005, 2043
Eam1104I	CTCTTC	2 cut(s)	55, 2910
Eam1105I	GACNNNNNGTC	1 cut(s)	39
EarI	CTCTTC	2 cut(s)	55, 2910
EciI	GGCGGA	2 cut(s)	922, 1443
Eco130I	CCWWGG	3 cut(s)	1753, 2892, 2922
Eco147I	AGGCCT	1 cut(s)	2977
Eco31I	GGTCTC	4 cut(s)	47, 990, 1676, 2912
Eco32I	GATATC	1 cut(s)	2572
Eco47I	GGWCC	6 cut(s)	483, 623, 695, 1795, 1949, 3137
Eco57I	CTGAAG	3 cut(s)	103, 1848, 3198
Eco81I	CCTNAGG	1 cut(s)	2972
Eco88I	CYCGRG	2 cut(s)	2219, 2705
EcoO109I	RGGNCCY	2 cut(s)	695, 911
EcoRI	GAATTC	2 cut(s)	2132, 2819
EcoRII	CCWGG	2 cut(s)	1262, 1782
EcoRV	GATATC	1 cut(s)	2572
EcoT14I	CCWWGG	3 cut(s)	1753, 2892, 2922
EcoT22I	ATGCAT	1 cut(s)	2962
ErhI	CCWWGG	3 cut(s)	1753, 2892, 2922
Esp3I	CGTCTC	1 cut(s)	1709
FalI	AAGNNNNNCTT	6 cut(s)	374, 406, 669, 701, 3018, 3050
FaqI	GGGAC	3 cut(s)	496, 1930, 2431
FauI	CCCGC	5 cut(s)	699, 864, 1002, 1632, 2667
FauNDI	CATATG	1 cut(s)	407
FbaI	TGATCA	1 cut(s)	723
FblI	GTMKAC	3 cut(s)	1776, 2244, 2826
Fnu4HI	GCNGC	9 cut(s)	127, 442, 1525, 2121, 2187, 2208, 2230, 2288, 2852
FokI	GGATG	5 cut(s)	1224, 1766, 1844, 1913, 2346
Fsp4HI	GCNGC	9 cut(s)	127, 442, 1525, 2121, 2187, 2208, 2230, 2288, 2852
FspBI	CTAG	4 cut(s)	731, 1293, 2054, 2517
GlaI	GCGC	3 cut(s)	584, 1611, 1638
GluI	GCNGC	9 cut(s)	127, 442, 1525, 2121, 2187, 2208, 2230, 2288, 2852
GsaI	CCCAGC	1 cut(s)	1830
HapII	CCGG	5 cut(s)	765, 1208, 2046, 3048, 3156
HgaI	GACGC	3 cut(s)	433, 877, 1691
HhaI	GCGC	3 cut(s)	585, 1612, 1639
Hin6I	GCGC	3 cut(s)	583, 1610, 1637
HinP1I	GCGC	3 cut(s)	583, 1610, 1637
HincII	GTYRAC	4 cut(s)	1122, 1306, 3129, 3210
HindII	GTYRAC	4 cut(s)	1122, 1306, 3129, 3210
HindIII	AAGCTT	4 cut(s)	257, 506, 2375, 2639
HpaII	CCGG	5 cut(s)	765, 1208, 2046, 3048, 3156
HphI	GGTGA	6 cut(s)	643, 754, 1139, 1252, 2264, 2293
HpyCH4IV	ACGT	3 cut(s)	63, 179, 273
HpyF10VI	GCNNNNNNNGC	3 cut(s)	251, 1823, 1832
HpySE526I	ACGT	3 cut(s)	63, 179, 273
HspAI	GCGC	3 cut(s)	583, 1610, 1637
Ksp22I	TGATCA	1 cut(s)	723
KspI	CCGCGG	1 cut(s)	707
LmnI	GCTCC	3 cut(s)	123, 2048, 2707
Lsp1109I	GCAGC	7 cut(s)	113, 1536, 2107, 2173, 2219, 2299, 2838
MaeI	CTAG	4 cut(s)	731, 1293, 2054, 2517
MaeII	ACGT	3 cut(s)	63, 179, 273
MaeIII	GTNAC	4 cut(s)	821, 1226, 2453, 2723
MflI	RGATCY	4 cut(s)	334, 1215, 1890, 2146
MhlI	GDGCHC	2 cut(s)	2053, 2954
MlsI	TGGCCA	1 cut(s)	2007
MluNI	TGGCCA	1 cut(s)	2007
MlyI	GAGTC	3 cut(s)	544, 1885, 2875
MmeI	TCCRAC	1 cut(s)	1174
Mox20I	TGGCCA	1 cut(s)	2007
Mph1103I	ATGCAT	1 cut(s)	2962
MroXI	GAANNNNTTC	1 cut(s)	1805
MscI	TGGCCA	1 cut(s)	2007
MslI	CAYNNNNRTG	1 cut(s)	2927
Msp20I	TGGCCA	1 cut(s)	2007
MspA1I	CMGCKG	2 cut(s)	706, 2290
MspI	CCGG	5 cut(s)	765, 1208, 2046, 3048, 3156
MspR9I	CCNGG	4 cut(s)	1209, 1264, 1784, 3049
Mva1269I	GAATGC	1 cut(s)	1292
MvaI	CCWGG	2 cut(s)	1264, 1784
MvnI	CGCG	5 cut(s)	444, 706, 871, 1639, 2674
MwoI	GCNNNNNNNGC	3 cut(s)	251, 1823, 1832
NciI	CCSGG	2 cut(s)	1209, 3049
NcoI	CCATGG	1 cut(s)	2922
NdeI	CATATG	1 cut(s)	407
NlaIV	GGNNCC	7 cut(s)	484, 697, 912, 1201, 1217, 2402, 2703
NmuCI	GTSAC	2 cut(s)	821, 2453
NsiI	ATGCAT	1 cut(s)	2962
NspI	RCATGY	3 cut(s)	195, 3004, 3127
NspV	TTCGAA	1 cut(s)	503
PaeI	GCATGC	1 cut(s)	3004
PagI	TCATGA	1 cut(s)	2085
PceI	AGGCCT	1 cut(s)	2977
PctI	GAATGC	1 cut(s)	1292
PdmI	GAANNNNTTC	1 cut(s)	1805
PfeI	GAWTC	8 cut(s)	472, 1013, 1556, 1616, 2014, 2543, 2556, 3203
PflMI	CCANNNNNTGG	1 cut(s)	526
PfoI	TCCNGGA	1 cut(s)	1782
PkrI	GCNGC	9 cut(s)	128, 443, 1526, 2122, 2188, 2209, 2231, 2289, 2853
PleI	GAGTC	3 cut(s)	543, 1884, 2874
PpsI	GAGTC	3 cut(s)	543, 1884, 2874
PpuMI	RGGWCCY	1 cut(s)	695
PshAI	GACNNNNGTC	1 cut(s)	534
Psp1406I	AACGTT	1 cut(s)	179
Psp5II	RGGWCCY	1 cut(s)	695
Psp6I	CCWGG	2 cut(s)	1262, 1782
PspFI	CCCAGC	1 cut(s)	1826
PspGI	CCWGG	2 cut(s)	1262, 1782
PspN4I	GGNNCC	7 cut(s)	484, 697, 912, 1201, 1217, 2402, 2703
PspPPI	RGGWCCY	1 cut(s)	695
PstI	CTGCAG	1 cut(s)	2125
PstNI	CAGNNNCTG	2 cut(s)	126, 2410
PsuI	RGATCY	4 cut(s)	334, 1215, 1890, 2146
PvuII	CAGCTG	1 cut(s)	2290
RsaI	GTAC	8 cut(s)	343, 556, 1628, 2058, 2426, 2906, 3115, 3164
RsaNI	GTAC	8 cut(s)	342, 555, 1627, 2057, 2425, 2905, 3114, 3163
RseI	CAYNNNNRTG	1 cut(s)	2927
SacII	CCGCGG	1 cut(s)	707
SatI	GCNGC	9 cut(s)	127, 442, 1525, 2121, 2187, 2208, 2230, 2288, 2852
ScaI	AGTACT	2 cut(s)	2058, 2906
SchI	GAGTC	3 cut(s)	544, 1885, 2875
ScrFI	CCNGG	4 cut(s)	1209, 1264, 1784, 3049
SduI	GDGCHC	2 cut(s)	2053, 2954
SfcI	CTRYAG	2 cut(s)	2121, 2803
Sfr303I	CCGCGG	1 cut(s)	707
SfuI	TTCGAA	1 cut(s)	503
SgrBI	CCGCGG	1 cut(s)	707
SinI	GGWCC	6 cut(s)	483, 623, 695, 1795, 1949, 3137
SmiMI	CAYNNNNRTG	1 cut(s)	2927
SmlI	CTYRAG	5 cut(s)	267, 532, 667, 905, 3193
SmoI	CTYRAG	5 cut(s)	267, 532, 667, 905, 3193
SpeI	ACTAGT	1 cut(s)	2053
SphI	GCATGC	1 cut(s)	3004
SseBI	AGGCCT	1 cut(s)	2977
SspI	AATATT	3 cut(s)	456, 1026, 1234
SspMI	CTAG	4 cut(s)	731, 1293, 2054, 2517
StuI	AGGCCT	1 cut(s)	2977
StyD4I	CCNGG	4 cut(s)	1207, 1262, 1782, 3047
StyI	CCWWGG	3 cut(s)	1753, 2892, 2922
TaiI	ACGT	3 cut(s)	66, 182, 276
TaqI	TCGA	8 cut(s)	503, 538, 891, 1809, 1874, 2478, 3006, 3043
TatI	WGTACW	6 cut(s)	341, 1626, 2056, 2424, 2904, 3113
TauI	GCSGC	2 cut(s)	444, 2232
TfiI	GAWTC	8 cut(s)	472, 1013, 1556, 1616, 2014, 2543, 2556, 3203
TscAI	CASTG	4 cut(s)	403, 789, 1201, 1416
TseFI	GTSAC	2 cut(s)	821, 2453
TseI	GCWGC	7 cut(s)	126, 1524, 2120, 2186, 2207, 2287, 2851
Tsp45I	GTSAC	2 cut(s)	821, 2453
TspDTI	ATGAA	9 cut(s)	41, 159, 844, 1299, 1376, 1476, 1528, 2346, 2661
TspGWI	ACGGA	1 cut(s)	1068
TspRI	CASTG	4 cut(s)	403, 789, 1201, 1416
Van91I	CCANNNNNTGG	1 cut(s)	526
VneI	GTGCAC	1 cut(s)	2950
VpaK11BI	GGWCC	6 cut(s)	483, 623, 695, 1795, 1949, 3137
XapI	RAATTY	5 cut(s)	1369, 2132, 2321, 2616, 2819
XceI	RCATGY	3 cut(s)	195, 3004, 3127
XmiI	GTMKAC	3 cut(s)	1776, 2244, 2826
XmnI	GAANNNNTTC	1 cut(s)	1805
XspI	CTAG	4 cut(s)	731, 1293, 2054, 2517
ZrmI	AGTACT	2 cut(s)	2058, 2906
Zsp2I	ATGCAT	1 cut(s)	2962

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.