Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0008419

Physical Location & Seq

Genomic Coordinates Forward (+)

5566181 .. 5571770

Gene Structure 5590 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2625 bp

ATGCATTTAGGTGTATTGGACTCTACGATCCTTGATCCACCAATTGGTCGACACTCCGCGCTGCCCAAACCAAACCCTCCAAACCCCAACACTCTCCCCTTCTCTCTCTCTCTAGCCATGGCTTCCGATTCTTCTCCGGCGGACTTCAACAACGGGCCTTCTTCTCCTGATGACTCCTTATCGAGCCCAATCGGCAACACCTTCTCCTCTCCGGCGGACGCCACCCACCGCAGGAGGCGTCGATCCATTACCCCCTCGGAATTTGACACCCCGCGGGCCCAGCGGTCCCGGTTCGCCGCATCCGAAACAACCCCAACGGGCACGCGCCGCGCCAACGGCCATAGTGCTGGCCCTGCCCCGACCCCCTCCTCCACCGACGGTGATGTCCCGCCATCTTCTGAAGGCGGCGACGGGTATGGCATGGATGAGGACCGACCCACATTTGTCTGGGGCACGAATATCAGCGTGAACGACGTTAAGGTGGCGATCGTTAGGTTTCTGAAGAATTTTCGGGATGAGTCGCAGTGGAATACGGAGAGTGAGTACCTTACAGAAGGGAAGTACATTGAGGCCATCAAGAGAGTTATTGAGATGGAAGATGATTCGCTTGATGTTGATGCACGCAATGTGTTTGATTATGATTCCGACTTGTATGCGAAGATGGTCAGGTACCCACTCGAGGTTCTCGCGATTTTTGATATTGTTTTGATGGAAATGCTGCCCATCATAAACCCGTTGTTTGAAAAGCACATCCAAACTCGGATTTATAATCTCAAAACATCCATATCGATGAGAAATCTCAACCCATCTGACATTGAGAGGATGGTTTCGTTGAAGGGAATGATTATTCGGTCTAGTTCAATAATACCAGAGATAAGGGAAGCAATATTTCGATGCCTCGTGTGTGGCTACTACTCCGACCCTCTTCCCGTAGAGAAAGGGAGAATATCTGAGCCTACAATATGCTTGAAGGAAGAGTGTCAAGCCAGGAATTCCATGACACTTGTTCATAATCGATGCAGGTTTACAGATAAACAGATTGTGAGGCTCCAGGAGACTCCTGATGAGATCCCTGAAGGAGGGACACCACATACGGTGAGCTTGTTGATGCATGACAAACTGGTTGATGCTGGAAAACCAGGAGACAGAATTGAGGTTACTGGGATATACAGGGCTATGACTGTTAGAGTTGGACCAACACAGAGAACTGTAAAATCACTGTTCAAGACTTACATTGATTGTCTGCATATAAAAAAATCTGATAAGTCAAGAATGCTGGCGGAGGATCAAGTGGAAGTTGACAATACCTTGGTTGGAAACTCAGAAGAGATTCTTTTTGATGAAAAAAAGGTGGAACAATTGAAAGAGCTCGCAAAACAGCCAGATATATATGATCGATTAACAAGGTCTTTAGCACCAAACATCTGGGAGTTAGATGATGTAAAAAAAGGCCTTCTTTGCCAGCTTTTTGGTGGGAATGCTTTGAAGCTTCCATCTGGTGCTCGCTTTCGTGGTGATATTAATATTCTTCTTGTTGGTGATCCTGGAACCAGCAAGTCCCAGCTGCTCCAGTACATACACAAGTTAGCGCCTCGCGGAATATACACTAGTGGAAGAGGGAGTTCTGCCGTTGGATTGACTGCTTATGTTGCGAACGATCCCGAAACAGGGGAAACAGTTTTAGAGAGTGGAGCCTTAGTTTTGAGTGATAGAGGCATTTGCTGCATTGACGAATTTGACAAAATGTCTGATAGTGCGAGGAGCATGCTACATGAGGTGATGGAACAGCAAACTGTTTCAATAGCCAAGGCAGGAATTATTGCTTCCCTTAATGCTAGGACTTCGGTCTTGGCTTGTGCAAATCCAAGTGGTTCACGCTATAACCCTCGCTTATCAGTAATTGACAACATACACCTTCCTCCTACCTTATTATCCAGGTTCGATCTGATCTACTTACTCCTTGACAAGGCTGATGAGCATACAGACAGACGCCTCGCAAAGCATATTGTTGCATTACACTTTGAGAATCCTGAGAGCGTACAGCAGGATGTCTTAGACATTGCTACGTTGACTGCATATGTGGGCTATGCCCGAAAGCATATTCACCCACAATTATCTGATGAGGCTGCTGAAGAGTTGACAAGAGGTTATGTTGAGTTGAGGAGGAGAGGAAATTTTCCAGGCAGTAGCAAAAAGGTGATAACAGCAACACCCAGGCAGATTGAGAGTTTGATACGACTTAGTGAAGCTCTAGCTCGCATCCGTTTCTCAGAATCGGTTGAGAAGCATGATGTTATTGAGGCATTTCGGCTTCTGGAAGTTGCAATGCAGCAATCGGCAACTGATCACTCCACAGGAACGATCGAGATCGATCTTATCACCACTGGAATTTCGGCAAGTGAAAGAATGAGACGAGAAAGTCTTGTATCATCAGCTCGCAACATAATTATGGAGAAGATGCAACTTGGGGGACCCTCAATGCGCTTGTTAGAGCTGCTGGAGGAGCTGAAGCAGCAGAGCTCTGGTGGCGAACTCCACCTCAATGATCTGAGGACTGCAATTACGACACTAGCAAGTGAGGGATTTGTGGCTTTTGTTCACGGTGACAGTGTCAAAAGAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

875

Amino Acids

96.99

Weight (kDa)

5.76

Isoelectric Point (pI)

52.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	163 - 257	4.6e-12	MCM N-terminal domain
MCM_OB	PF17207	264 - 393	6.6e-39	MCM OB domain
MCM	PF00493	451 - 673	3.5e-100	MCM P-loop domain
AAA_5	PF07728	508 - 624	7.6e-07	AAA domain (dynein-related subfamily)
MCM_lid	PF17855	689 - 775	3.7e-26	MCM AAA-lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008419)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16440
fragaria_vesca	FvH4_2g09990
malus_domestica	MD00G1085600.v1.1 MD10G1066500.v1.1
prunus_persica	Prupe.8G088400_v2.0.a1
pyrus_communis	pycom05g04970 pycom05g04980 pycom10g05200
rosa_chinensis	RchiOBHm_Chr6g0266881 RchiOBHm_Chr6g0266891
rosa_laevigata	RLG00000014063 RLG00000014064 RLG00000014065
rosa_multiflora	Rmu_co8507775.1_g000001 Rmu_sc0021142.1_g000001 Rmu_sc0036177.1_g000001
rosa_roxburghii	Rroxscaffold_7G00201330
rosa_rugosa	Rorug06G0027900
rosa_samantha	Rh6AG149000 Rh6BG150000 Rh6CG144900 Rh6DG134200
rosa_wichuraiana	Rw6G012890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	768
AasI	GACNNNNNNGTC	2 cut(s)	383, 2418
Acc36I	ACCTGC	1 cut(s)	1011
Acc65I	GGTACC	1 cut(s)	669
AccB1I	GGYRCC	1 cut(s)	669
AccB7I	CCANNNNNTGG	1 cut(s)	44
AccI	GTMKAC	1 cut(s)	49
AccII	CGCG	6 cut(s)	59, 274, 325, 330, 689, 1596
AclWI	GGATC	7 cut(s)	22, 29, 237, 1063, 1293, 1535, 1652
AcoI	YGGCCR	1 cut(s)	337
AcsI	RAATTY	6 cut(s)	260, 505, 991, 1733, 2173, 2388
AcuI	CTGAAG	5 cut(s)	420, 521, 1095, 2151, 2528
AcyI	GRCGYC	3 cut(s)	219, 238, 1990
AfaI	GTAC	5 cut(s)	545, 563, 671, 1574, 2040
AfiI	CCNNNNNNNGG	7 cut(s)	44, 231, 679, 1079, 1667, 1668, 1966
AgsI	TTSAA	9 cut(s)	148, 743, 835, 861, 970, 1225, 1363, 1486, 1800
AhdI	GACNNNNNGTC	1 cut(s)	1744
AhlI	ACTAGT	1 cut(s)	1607
AjnI	CCWGG	7 cut(s)	986, 1050, 1138, 1543, 1934, 2179, 2213
Alw21I	GWGCWC	3 cut(s)	1371, 1504, 2522
Alw26I	GTCTC	3 cut(s)	1049, 1137, 2404
AlwI	GGATC	7 cut(s)	22, 29, 237, 1063, 1293, 1535, 1652
Ama87I	CYCGRG	1 cut(s)	677
AoxI	GGCC	6 cut(s)	155, 276, 337, 349, 570, 1450
ApaI	GGGCCC	1 cut(s)	280
ApeKI	GCWGC	8 cut(s)	61, 718, 1564, 1722, 2126, 2329, 2494, 2512
ApoI	RAATTY	6 cut(s)	260, 505, 991, 1733, 2173, 2388
AseI	ATTAAT	1 cut(s)	1521
Asp700I	GAANNNNTTC	1 cut(s)	1329
Asp718I	GGTACC	1 cut(s)	669
AspLEI	GCGC	5 cut(s)	61, 327, 332, 1591, 2484
AspS9I	GGNCC	8 cut(s)	155, 276, 277, 285, 350, 430, 1193, 2471
AsuC2I	CCSGG	1 cut(s)	289
AsuHPI	GGTGA	9 cut(s)	392, 1108, 1526, 1550, 1789, 2096, 2209, 2371, 2615
AvaI	CYCGRG	1 cut(s)	677
AvaII	GGWCC	4 cut(s)	285, 430, 1193, 2471
BaeGI	GKGCMC	3 cut(s)	280, 323, 455
BanI	GGYRCC	1 cut(s)	669
BanII	GRGCYC	4 cut(s)	188, 280, 1371, 2522
BauI	CACGAG	1 cut(s)	899
Bbv12I	GWGCWC	3 cut(s)	1371, 1504, 2522
BbvI	GCAGC	8 cut(s)	48, 705, 1551, 1709, 2113, 2341, 2481, 2524
BceAI	ACGGC	2 cut(s)	352, 1613
BcgI	CGANNNNNNTGC	4 cut(s)	635, 669, 1747, 1781
BciT130I	CCWGG	7 cut(s)	988, 1052, 1140, 1545, 1936, 2181, 2215
BclI	TGATCA	1 cut(s)	2344
BcnI	CCSGG	1 cut(s)	289
BcoDI	GTCTC	3 cut(s)	1049, 1137, 2404
BcuI	ACTAGT	1 cut(s)	1607
BfaI	CTAG	6 cut(s)	113, 855, 1608, 1836, 2252, 2570
BfoI	RGCGCY	1 cut(s)	1592
BfuAI	ACCTGC	1 cut(s)	1011
BglI	GCCNNNNNGGC	1 cut(s)	192
Bme1390I	CCNGG	8 cut(s)	289, 988, 1052, 1140, 1545, 1936, 2181, 2215
Bme18I	GGWCC	4 cut(s)	285, 430, 1193, 2471
BmeRI	GACNNNNNGTC	1 cut(s)	1744
BmeT110I	CYCGRG	1 cut(s)	677
BmgT120I	GGNCC	8 cut(s)	155, 276, 277, 285, 350, 430, 1193, 2471
BmiI	GGNNCC	8 cut(s)	278, 287, 671, 1049, 1549, 1693, 2472, 2473
BmrFI	CCNGG	8 cut(s)	289, 988, 1052, 1140, 1545, 1936, 2181, 2215
BmrI	ACTGGG	1 cut(s)	1170
BmsI	GCATC	8 cut(s)	308, 607, 884, 1007, 1098, 1117, 2268, 2448
BmuI	ACTGGG	1 cut(s)	1170
BoxI	GACNNNNGTC	1 cut(s)	1844
BpmI	CTGGAG	3 cut(s)	1034, 1553, 2519
BpuMI	CCSGG	1 cut(s)	289
Bsa29I	ATCGAT	4 cut(s)	788, 1015, 1396, 2370
BsaBI	GATNNNNATC	1 cut(s)	2366
BsaHI	GRCGYC	3 cut(s)	219, 238, 1990
BsaJI	CCNNGG	6 cut(s)	117, 255, 272, 1308, 1806, 2213
BsaXI	ACNNNNNCTCC	6 cut(s)	188, 218, 527, 557, 1683, 1713
Bsc4I	CCNNNNNNNGG	7 cut(s)	44, 231, 679, 1079, 1667, 1668, 1966
Bse1I	ACTGG	4 cut(s)	1125, 1165, 1570, 2389
Bse3DI	GCAATG	3 cut(s)	631, 2058, 2331
Bse8I	GATNNNNATC	1 cut(s)	2366
BseBI	CCWGG	7 cut(s)	988, 1052, 1140, 1545, 1936, 2181, 2215
BseCI	ATCGAT	4 cut(s)	788, 1015, 1396, 2370
BseDI	CCNNGG	6 cut(s)	117, 255, 272, 1308, 1806, 2213
BseGI	GGATG	8 cut(s)	299, 430, 520, 750, 779, 828, 2053, 2259
BseJI	GATNNNNATC	1 cut(s)	2366
BseLI	CCNNNNNNNGG	7 cut(s)	44, 231, 679, 1079, 1667, 1668, 1966
BseMI	GCAATG	3 cut(s)	631, 2058, 2331
BseMII	CTCAG	5 cut(s)	942, 1335, 2022, 2283, 2540
BseNI	ACTGG	4 cut(s)	1125, 1165, 1570, 2389
BseRI	GAGGAG	6 cut(s)	196, 358, 1774, 2176, 2179, 2516
BseSI	GKGCMC	3 cut(s)	280, 323, 455
BseXI	GCAGC	8 cut(s)	48, 705, 1551, 1709, 2113, 2341, 2481, 2524
BseYI	CCCAGC	2 cut(s)	279, 1560
Bsh1236I	CGCG	6 cut(s)	59, 274, 325, 330, 689, 1596
Bsh1285I	CGRYCG	2 cut(s)	489, 2364
BshFI	GGCC	6 cut(s)	157, 278, 339, 351, 572, 1452
BshNI	GGYRCC	1 cut(s)	669
BshVI	ATCGAT	4 cut(s)	788, 1015, 1396, 2370
BsiEI	CGRYCG	2 cut(s)	489, 2364
BsiHKAI	GWGCWC	3 cut(s)	1371, 1504, 2522
BsiHKCI	CYCGRG	1 cut(s)	677
BsiSI	CCGG	3 cut(s)	137, 212, 289
BslFI	GGGAC	5 cut(s)	271, 371, 1096, 1543, 2484
BslI	CCNNNNNNNGG	7 cut(s)	44, 231, 679, 1079, 1667, 1668, 1966
BsmAI	GTCTC	3 cut(s)	1049, 1137, 2404
BsmBI	CGTCTC	1 cut(s)	2404
BsmFI	GGGAC	5 cut(s)	271, 371, 1096, 1543, 2484
BsmI	GAATGC	2 cut(s)	1278, 1483
BsnI	GGCC	6 cut(s)	157, 278, 339, 351, 572, 1452
BsoBI	CYCGRG	1 cut(s)	677
Bsp120I	GGGCCC	1 cut(s)	276
Bsp1286I	GDGCHC	7 cut(s)	188, 280, 323, 455, 1371, 1504, 2522
Bsp19I	CCATGG	1 cut(s)	117
Bsp68I	TCGCGA	1 cut(s)	689
BspANI	GGCC	6 cut(s)	157, 278, 339, 351, 572, 1452
BspCNI	CTCAG	5 cut(s)	943, 1334, 2023, 2282, 2541
BspDI	ATCGAT	4 cut(s)	788, 1015, 1396, 2370
BspFNI	CGCG	6 cut(s)	59, 274, 325, 330, 689, 1596
BspLI	GGNNCC	8 cut(s)	278, 287, 671, 1049, 1549, 1693, 2472, 2473
BspMI	ACCTGC	1 cut(s)	1011
BspPI	GGATC	7 cut(s)	22, 29, 237, 1063, 1293, 1535, 1652
BspT107I	GGYRCC	1 cut(s)	669
BsrDI	GCAATG	3 cut(s)	631, 2058, 2331
BsrI	ACTGG	4 cut(s)	1125, 1165, 1570, 2389
BssECI	CCNNGG	6 cut(s)	117, 255, 272, 1308, 1806, 2213
BssNI	GRCGYC	3 cut(s)	219, 238, 1990
BssSI	CACGAG	1 cut(s)	899
BssT1I	CCWWGG	3 cut(s)	117, 1308, 1806
Bst2BI	CACGAG	1 cut(s)	899
Bst2UI	CCWGG	7 cut(s)	988, 1052, 1140, 1545, 1936, 2181, 2215
Bst4CI	ACNGT	9 cut(s)	380, 1096, 1183, 1210, 1221, 1678, 1795, 2603, 2609
Bst6I	CTCTTC	5 cut(s)	930, 969, 1320, 1609, 2127
BstACI	GRCGYC	3 cut(s)	219, 238, 1990
BstAPI	GCANNNNNTGC	1 cut(s)	1722
BstDEI	CTNAG	8 cut(s)	951, 1321, 1696, 2031, 2053, 2240, 2269, 2549
BstDSI	CCRYGG	2 cut(s)	117, 272
BstENI	CCTNNNNNAGG	2 cut(s)	1077, 1964
BstF5I	GGATG	8 cut(s)	299, 430, 520, 750, 779, 828, 2053, 2259
BstFNI	CGCG	6 cut(s)	59, 274, 325, 330, 689, 1596
BstH2I	RGCGCY	1 cut(s)	1592
BstHHI	GCGC	5 cut(s)	61, 327, 332, 1591, 2484
BstMAI	GTCTC	3 cut(s)	1049, 1137, 2404
BstMCI	CGRYCG	2 cut(s)	489, 2364
BstNI	CCWGG	7 cut(s)	988, 1052, 1140, 1545, 1936, 2181, 2215
BstNSI	RCATGY	1 cut(s)	1768
BstPAI	GACNNNNGTC	1 cut(s)	1844
BstSCI	CCNGG	8 cut(s)	287, 986, 1050, 1138, 1543, 1934, 2179, 2213
BstSLI	GKGCMC	3 cut(s)	280, 323, 455
BstUI	CGCG	6 cut(s)	59, 274, 325, 330, 689, 1596
BstV1I	GCAGC	8 cut(s)	48, 705, 1551, 1709, 2113, 2341, 2481, 2524
BstX2I	RGATCY	1 cut(s)	1068
BstXI	CCANNNNNNTGG	3 cut(s)	347, 1425, 1469
BstYI	RGATCY	1 cut(s)	1068
Bsu15I	ATCGAT	4 cut(s)	788, 1015, 1396, 2370
BsuRI	GGCC	6 cut(s)	157, 278, 339, 351, 572, 1452
BsuTUI	ATCGAT	4 cut(s)	788, 1015, 1396, 2370
BtgI	CCRYGG	2 cut(s)	117, 272
BtsCI	GGATG	8 cut(s)	299, 430, 520, 750, 779, 828, 2053, 2259
BtsI	GCAGTG	1 cut(s)	530
BtsIMutI	CAGTG	4 cut(s)	530, 1217, 2382, 2614
BtuMI	TCGCGA	1 cut(s)	689
BveI	ACCTGC	1 cut(s)	1011
CfoI	GCGC	5 cut(s)	61, 327, 332, 1591, 2484
Cfr13I	GGNCC	8 cut(s)	155, 276, 277, 285, 350, 430, 1193, 2471
Cfr42I	CCGCGG	1 cut(s)	275
ClaI	ATCGAT	4 cut(s)	788, 1015, 1396, 2370
CseI	GACGC	3 cut(s)	227, 227, 1998
Csp6I	GTAC	5 cut(s)	544, 562, 670, 1573, 2039
CviAII	CATG	7 cut(s)	118, 421, 997, 1112, 1765, 1772, 2288
CviQI	GTAC	5 cut(s)	544, 562, 670, 1573, 2039
DdeI	CTNAG	8 cut(s)	951, 1321, 1696, 2031, 2053, 2240, 2269, 2549
DrdI	GACNNNNNNGTC	2 cut(s)	383, 2418
DriI	GACNNNNNGTC	1 cut(s)	1744
DseDI	GACNNNNNNGTC	2 cut(s)	383, 2418
EaeI	YGGCCR	1 cut(s)	337
Eam1104I	CTCTTC	5 cut(s)	930, 969, 1320, 1609, 2127
Eam1105I	GACNNNNNGTC	1 cut(s)	1744
EarI	CTCTTC	5 cut(s)	930, 969, 1320, 1609, 2127
EciI	GGCGGA	3 cut(s)	155, 230, 1295
Ecl136II	GAGCTC	2 cut(s)	1369, 2520
Eco130I	CCWWGG	3 cut(s)	117, 1308, 1806
Eco147I	AGGCCT	1 cut(s)	1452
Eco24I	GRGCYC	4 cut(s)	188, 280, 1371, 2522
Eco47I	GGWCC	4 cut(s)	285, 430, 1193, 2471
Eco53kI	GAGCTC	2 cut(s)	1369, 2520
Eco57I	CTGAAG	5 cut(s)	420, 521, 1095, 2151, 2528
Eco88I	CYCGRG	1 cut(s)	677
EcoICRI	GAGCTC	2 cut(s)	1369, 2520
EcoNI	CCTNNNNNAGG	2 cut(s)	1077, 1964
EcoO109I	RGGNCCY	1 cut(s)	2471
EcoRI	GAATTC	1 cut(s)	991
EcoRII	CCWGG	7 cut(s)	986, 1050, 1138, 1543, 1934, 2179, 2213
EcoT14I	CCWWGG	3 cut(s)	117, 1308, 1806
EcoT22I	ATGCAT	2 cut(s)	6, 1113
EcoT38I	GRGCYC	4 cut(s)	188, 280, 1371, 2522
ErhI	CCWWGG	3 cut(s)	117, 1308, 1806
Esp3I	CGTCTC	1 cut(s)	2404
FaeI	CATG	7 cut(s)	121, 424, 1000, 1115, 1768, 1775, 2291
FalI	AAGNNNNNCTT	4 cut(s)	1317, 1349, 1440, 1472
FaqI	GGGAC	5 cut(s)	271, 371, 1096, 1543, 2484
FatI	CATG	7 cut(s)	117, 420, 996, 1111, 1764, 1771, 2287
FauI	CCCGC	3 cut(s)	267, 279, 396
FauNDI	CATATG	1 cut(s)	2077
FbaI	TGATCA	1 cut(s)	2344
FblI	GTMKAC	1 cut(s)	49
FokI	GGATG	8 cut(s)	286, 437, 527, 737, 766, 835, 2060, 2246
FriOI	GRGCYC	4 cut(s)	188, 280, 1371, 2522
FspBI	CTAG	6 cut(s)	113, 855, 1608, 1836, 2252, 2570
GlaI	GCGC	5 cut(s)	60, 326, 331, 1590, 2483
GsaI	CCCAGC	2 cut(s)	283, 1564
GsuI	CTGGAG	3 cut(s)	1034, 1553, 2519
HaeII	RGCGCY	1 cut(s)	1592
HaeIII	GGCC	6 cut(s)	157, 278, 339, 351, 572, 1452
HapII	CCGG	3 cut(s)	137, 212, 289
HgaI	GACGC	3 cut(s)	227, 227, 1998
HhaI	GCGC	5 cut(s)	61, 327, 332, 1591, 2484
Hin1I	GRCGYC	3 cut(s)	219, 238, 1990
Hin1II	CATG	7 cut(s)	121, 424, 1000, 1115, 1768, 1775, 2291
Hin6I	GCGC	5 cut(s)	59, 325, 330, 1589, 2482
HinP1I	GCGC	5 cut(s)	59, 325, 330, 1589, 2482
HincII	GTYRAC	4 cut(s)	50, 1300, 2070, 2139
HindII	GTYRAC	4 cut(s)	50, 1300, 2070, 2139
HindIII	AAGCTT	1 cut(s)	1487
HpaII	CCGG	3 cut(s)	137, 212, 289
HphI	GGTGA	9 cut(s)	392, 1108, 1526, 1550, 1789, 2096, 2209, 2371, 2615
Hpy166II	GTNNAC	8 cut(s)	50, 469, 1026, 1300, 1874, 2070, 2139, 2599
Hpy8I	GTNNAC	8 cut(s)	50, 469, 1026, 1300, 1874, 2070, 2139, 2599
Hpy99I	CGWCG	4 cut(s)	243, 380, 413, 476
HpyCH4III	ACNGT	9 cut(s)	380, 1096, 1183, 1210, 1221, 1678, 1795, 2603, 2609
HpyCH4IV	ACGT	2 cut(s)	474, 2066
HpyF3I	CTNAG	8 cut(s)	951, 1321, 1696, 2031, 2053, 2240, 2269, 2549
HpySE526I	ACGT	2 cut(s)	474, 2066
Hsp92I	GRCGYC	3 cut(s)	219, 238, 1990
Hsp92II	CATG	7 cut(s)	121, 424, 1000, 1115, 1768, 1775, 2291
HspAI	GCGC	5 cut(s)	59, 325, 330, 1589, 2482
KflI	GGGWCCC	1 cut(s)	2471
KpnI	GGTACC	1 cut(s)	673
Ksp22I	TGATCA	1 cut(s)	2344
KspI	CCGCGG	1 cut(s)	275
LmnI	GCTCC	5 cut(s)	1053, 1572, 1691, 1761, 2503
Lsp1109I	GCAGC	8 cut(s)	48, 705, 1551, 1709, 2113, 2341, 2481, 2524
LweI	GCATC	8 cut(s)	308, 607, 884, 1007, 1098, 1117, 2268, 2448
MaeI	CTAG	6 cut(s)	113, 855, 1608, 1836, 2252, 2570
MaeII	ACGT	2 cut(s)	474, 2066
MaeIII	GTNAC	2 cut(s)	1156, 2603
MfeI	CAATTG	2 cut(s)	42, 1358
MflI	RGATCY	1 cut(s)	1068
MhlI	GDGCHC	7 cut(s)	188, 280, 323, 455, 1371, 1504, 2522
MlyI	GAGTC	4 cut(s)	14, 167, 527, 1051
MmeI	TCCRAC	5 cut(s)	669, 942, 1171, 1294, 1612
Mph1103I	ATGCAT	2 cut(s)	6, 1113
MroXI	GAANNNNTTC	1 cut(s)	1329
MseI	TTAA	4 cut(s)	477, 1400, 1521, 1830
MslI	CAYNNNNRTG	4 cut(s)	9, 788, 2447, 2541
MspA1I	CMGCKG	3 cut(s)	274, 283, 1564
MspI	CCGG	3 cut(s)	137, 212, 289
MspR9I	CCNGG	8 cut(s)	289, 988, 1052, 1140, 1545, 1936, 2181, 2215
MteI	GCGCNGCGC	1 cut(s)	328
MunI	CAATTG	2 cut(s)	42, 1358
Mva1269I	GAATGC	2 cut(s)	1278, 1483
MvaI	CCWGG	7 cut(s)	988, 1052, 1140, 1545, 1936, 2181, 2215
MvnI	CGCG	6 cut(s)	59, 274, 325, 330, 689, 1596
NciI	CCSGG	1 cut(s)	289
NcoI	CCATGG	1 cut(s)	117
NdeI	CATATG	1 cut(s)	2077
NlaIII	CATG	7 cut(s)	121, 424, 1000, 1115, 1768, 1775, 2291
NlaIV	GGNNCC	8 cut(s)	278, 287, 671, 1049, 1549, 1693, 2472, 2473
NmuCI	GTSAC	1 cut(s)	2603
NruI	TCGCGA	1 cut(s)	689
NsiI	ATGCAT	2 cut(s)	6, 1113
NspI	RCATGY	1 cut(s)	1768
PaeI	GCATGC	1 cut(s)	1768
PaeR7I	CTCGAG	1 cut(s)	677
PceI	AGGCCT	1 cut(s)	1452
PcsI	WCGNNNNNNNCGW	1 cut(s)	300
PctI	GAATGC	2 cut(s)	1278, 1483
PdmI	GAANNNNTTC	1 cut(s)	1329
PfeI	GAWTC	6 cut(s)	128, 602, 641, 1330, 2026, 2273
PflFI	GACNNNGTC	1 cut(s)	2609
PflMI	CCANNNNNTGG	1 cut(s)	44
PfoI	TCCNGGA	2 cut(s)	1050, 1543
Ple19I	CGATCG	2 cut(s)	489, 2364
PleI	GAGTC	4 cut(s)	14, 167, 526, 1051
PpsI	GAGTC	4 cut(s)	14, 167, 526, 1051
PpuMI	RGGWCCY	1 cut(s)	2471
PshAI	GACNNNNGTC	1 cut(s)	1844
PshBI	ATTAAT	1 cut(s)	1521
PsiI	TTATAA	1 cut(s)	768
Psp124BI	GAGCTC	2 cut(s)	1371, 2522
Psp5II	RGGWCCY	1 cut(s)	2471
Psp6I	CCWGG	7 cut(s)	986, 1050, 1138, 1543, 1934, 2179, 2213
PspFI	CCCAGC	2 cut(s)	279, 1560
PspGI	CCWGG	7 cut(s)	986, 1050, 1138, 1543, 1934, 2179, 2213
PspN4I	GGNNCC	8 cut(s)	278, 287, 671, 1049, 1549, 1693, 2472, 2473
PspOMI	GGGCCC	1 cut(s)	276
PspPI	GGNCC	8 cut(s)	155, 276, 277, 285, 350, 430, 1193, 2471
PspPPI	RGGWCCY	1 cut(s)	2471
PspXI	VCTCGAGB	1 cut(s)	677
PsuI	RGATCY	1 cut(s)	1068
PsyI	GACNNNGTC	1 cut(s)	2609
PvuI	CGATCG	2 cut(s)	489, 2364
PvuII	CAGCTG	1 cut(s)	1564
RruI	TCGCGA	1 cut(s)	689
RsaI	GTAC	5 cut(s)	545, 563, 671, 1574, 2040
RsaNI	GTAC	5 cut(s)	544, 562, 670, 1573, 2039
RseI	CAYNNNNRTG	4 cut(s)	9, 788, 2447, 2541
SacI	GAGCTC	2 cut(s)	1371, 2522
SacII	CCGCGG	1 cut(s)	275
SalI	GTCGAC	1 cut(s)	48
SaqAI	TTAA	4 cut(s)	477, 1400, 1521, 1830
Sau96I	GGNCC	8 cut(s)	155, 276, 277, 285, 350, 430, 1193, 2471
SchI	GAGTC	4 cut(s)	14, 167, 527, 1051
ScrFI	CCNGG	8 cut(s)	289, 988, 1052, 1140, 1545, 1936, 2181, 2215
SduI	GDGCHC	7 cut(s)	188, 280, 323, 455, 1371, 1504, 2522
SfaNI	GCATC	8 cut(s)	308, 607, 884, 1007, 1098, 1117, 2268, 2448
Sfr274I	CTCGAG	1 cut(s)	677
Sfr303I	CCGCGG	1 cut(s)	275
SgrBI	CCGCGG	1 cut(s)	275
SinI	GGWCC	4 cut(s)	285, 430, 1193, 2471
SlaI	CTCGAG	1 cut(s)	677
SmiMI	CAYNNNNRTG	4 cut(s)	9, 788, 2447, 2541
SmlI	CTYRAG	1 cut(s)	677
SmoI	CTYRAG	1 cut(s)	677
SpeI	ACTAGT	1 cut(s)	1607
SphI	GCATGC	1 cut(s)	1768
SseBI	AGGCCT	1 cut(s)	1452
SspI	AATATT	2 cut(s)	888, 1525
SspMI	CTAG	6 cut(s)	113, 855, 1608, 1836, 2252, 2570
SstI	GAGCTC	2 cut(s)	1371, 2522
StuI	AGGCCT	1 cut(s)	1452
StyD4I	CCNGG	8 cut(s)	287, 986, 1050, 1138, 1543, 1934, 2179, 2213
StyI	CCWWGG	3 cut(s)	117, 1308, 1806
TaaI	ACNGT	9 cut(s)	380, 1096, 1183, 1210, 1221, 1678, 1795, 2603, 2609
TaiI	ACGT	2 cut(s)	477, 2069
TaqII	GACCGA	3 cut(s)	447, 840, 1834
TatI	WGTACW	2 cut(s)	561, 1572
TauI	GCSGC	3 cut(s)	299, 330, 408
TfiI	GAWTC	6 cut(s)	128, 602, 641, 1330, 2026, 2273
Tru1I	TTAA	4 cut(s)	477, 1400, 1521, 1830
Tru9I	TTAA	4 cut(s)	477, 1400, 1521, 1830
TscAI	CASTG	4 cut(s)	530, 1224, 2389, 2614
TseFI	GTSAC	1 cut(s)	2603
TseI	GCWGC	8 cut(s)	61, 718, 1564, 1722, 2126, 2329, 2494, 2512
Tsp45I	GTSAC	1 cut(s)	2603
TspDTI	ATGAA	2 cut(s)	998, 1356
TspGWI	ACGGA	2 cut(s)	548, 2252
TspRI	CASTG	4 cut(s)	530, 1224, 2389, 2614
Tth111I	GACNNNGTC	1 cut(s)	2609
Van91I	CCANNNNNTGG	1 cut(s)	44
VpaK11BI	GGWCC	4 cut(s)	285, 430, 1193, 2471
VspI	ATTAAT	1 cut(s)	1521
XagI	CCTNNNNNAGG	2 cut(s)	1077, 1964
XapI	RAATTY	6 cut(s)	260, 505, 991, 1733, 2173, 2388
XceI	RCATGY	1 cut(s)	1768
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1469
XhoI	CTCGAG	1 cut(s)	677
XmiI	GTMKAC	1 cut(s)	49
XmnI	GAANNNNTTC	1 cut(s)	1329
XspI	CTAG	6 cut(s)	113, 855, 1608, 1836, 2252, 2570
Zsp2I	ATGCAT	2 cut(s)	6, 1113

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.