pycom10g07980

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009109
Physical Location & Seq
Reverse (-)
10086473 .. 10088636
2164 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1134 bp
ATGGAGATCACCCCGTCCCCGCCGCCGTCCACGTCGTCGTCTCAGGAGAACGAAGCGAGCGCCGCGGCGGTGACGGTCAAGTGCGACGATGAGGAAGAGGAGGAGGAAGTGTGCCGGATCTGTAGAAACACGGGCGACGCTGATAACCCGCTGCAGTACCCGTGCGCTTGTAGCGGAAGCATCAAGTTTGTGCACCAGGATTGCCTCATGCAGTGGCTTAATCACAGCAATGCTCGCCAATGCGAGGTTTGCAAGCATGCGTTTTCCTTCTCTCCTGTATATGCTGAGAATGCCCCTGCAAGGCTTCCTTTTCAAGAGTTTGTAGTTGGGATGGCAATGAAAACTTGCCATGTTCTGCAATTCCTTCTGCGCCTTAGTTTTGTGCTTTCTGTTTGGCTTCTCATTATACCTTTTATCACATTTTGGATATGGCGGTTGGCTTTTGTGAGGAGTTTTGGTGAAGCCCAGAGATTATTCCTTAGTCATTTATCCACTACAGTCATCCTTACTGATTGTCTGCATGGGTTCCTACTTTCTGCTAGCATTGTGTTGATTTTTCTTGGGGCCACTTCTCTGAGGGATTACTTCAGGCATTTACGTGAGCTTGGAGGACAGGATGCTGACAGAGAAGATGAAGGGGAAAGAAATGGTGCCCATGCTGCAAGGAGAGCTCCTGGACAAGCTAACAGGAACTTTGTTGGTGATGTGAATGGGGAAGATGCTGGAGCACAGGGGATCGCCGGAGCAGGTCAAATGATTAGGAGGAATGCAGAAAATGTTGCTGCTCGGTGGGAGATGCAGGCAGCTCGTCTTGAAGCTCATGTGGAGCAGATGTTTGATGGTCTGGATGATGCTGATGGGGCCGAGGATGTACCGTTTGATGAGCTTGTCGGCATGCAGGGACCTGTATTTCATTTAGTTGAAAATGCATTCACTGTTCTGGCCAGCAATATGATATTCCTTGGTGTAGTAATCTTTGTGCCTTTCTCAATAGGTCGGATTTTTGTTTTGGATTTTCTCCACAGCTACTGGTCCAGTATTGTCAACAGTCATGCCACTTACAGAATCAGCCCTTTCCTTAGCAAATGTAACATTGAAGAATGCAGTAACAGCGGTAACGAATGTATCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

378

Amino Acids

41.88

Weight (kDa)

4.94

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 38 - 84 9.5e-18 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 737
AccB1I GGYRCC 1 cut(s) 650
AccII CGCG 1 cut(s) 65
AciI CCGC 9 cut(s) 20, 23, 63, 65, 68, 149, 174, 433, 1113
AclWI GGATC 2 cut(s) 125, 743
AcoI YGGCCR 1 cut(s) 942
AcuI CTGAAG 1 cut(s) 571
AfaI GTAC 2 cut(s) 158, 873
AfiI CCNNNNNNNGG 2 cut(s) 244, 300
AgsI TTSAA 4 cut(s) 314, 815, 923, 1097
AjiI CACGTC 1 cut(s) 33
AjnI CCWGG 2 cut(s) 195, 673
AluBI AGCT 7 cut(s) 604, 671, 683, 806, 818, 886, 1026
AluI AGCT 7 cut(s) 604, 671, 683, 806, 818, 886, 1026
Alw21I GWGCWC 3 cut(s) 195, 673, 730
Alw26I GTCTC 1 cut(s) 45
Alw44I GTGCAC 1 cut(s) 191
AlwI GGATC 2 cut(s) 125, 743
AlwNI CAGNNNCTG 1 cut(s) 1029
AoxI GGCC 3 cut(s) 564, 861, 942
ApaLI GTGCAC 1 cut(s) 191
ApeKI GCWGC 4 cut(s) 151, 659, 782, 803
AspLEI GCGC 3 cut(s) 62, 167, 372
AspS9I GGNCC 4 cut(s) 564, 861, 902, 1032
AsuHPI GGTGA 3 cut(s) 82, 470, 713
AsuNHI GCTAGC 1 cut(s) 539
AvaII GGWCC 2 cut(s) 902, 1032
BaeGI GKGCMC 2 cut(s) 195, 655
BaeI ACNNNNGTAYC 1 cut(s) 1108
BalI TGGCCA 1 cut(s) 944
BanI GGYRCC 1 cut(s) 650
BanII GRGCYC 1 cut(s) 673
Bbv12I GWGCWC 3 cut(s) 195, 673, 730
BbvI GCAGC 4 cut(s) 138, 646, 769, 815
BccI CCATC 3 cut(s) 325, 833, 851
BceAI ACGGC 1 cut(s) 10
BcgI CGANNNNNNTGC 2 cut(s) 788, 822
BciT130I CCWGG 2 cut(s) 197, 675
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 3 cut(s) 121, 152, 495
BfoI RGCGCY 1 cut(s) 63
BfuAI ACCTGC 1 cut(s) 737
BisI GCNGC 7 cut(s) 23, 63, 66, 152, 660, 783, 804
BlsI GCNGC 7 cut(s) 24, 64, 67, 153, 661, 784, 805
Bme1390I CCNGG 2 cut(s) 197, 675
Bme18I GGWCC 2 cut(s) 902, 1032
BmgBI CACGTC 1 cut(s) 33
BmgT120I GGNCC 4 cut(s) 564, 861, 902, 1032
BmiI GGNNCC 5 cut(s) 527, 565, 652, 862, 903
BmrFI CCNGG 2 cut(s) 197, 675
BmsI GCATC 5 cut(s) 189, 607, 709, 786, 841
BmtI GCTAGC 1 cut(s) 543
BpmI CTGGAG 1 cut(s) 744
Bpu10I CCTNAGC 1 cut(s) 1079
BsaAI YACGTR 1 cut(s) 599
BsaJI CCNNGG 3 cut(s) 63, 864, 961
BsaXI ACNNNNNCTCC 6 cut(s) 95, 125, 442, 472, 818, 848
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 300
Bse1I ACTGG 2 cut(s) 1034, 1035
Bse3DI GCAATG 2 cut(s) 235, 342
BseBI CCWGG 2 cut(s) 197, 675
BseDI CCNNGG 3 cut(s) 63, 864, 961
BseGI GGATG 5 cut(s) 336, 501, 622, 853, 874
BseLI CCNNNNNNNGG 2 cut(s) 244, 300
BseMI GCAATG 2 cut(s) 235, 342
BseMII CTCAG 3 cut(s) 56, 276, 566
BseNI ACTGG 2 cut(s) 1034, 1035
BseRI GAGGAG 3 cut(s) 113, 116, 463
BseSI GKGCMC 2 cut(s) 195, 655
BseXI GCAGC 4 cut(s) 138, 646, 769, 815
Bsh1236I CGCG 1 cut(s) 65
BshFI GGCC 3 cut(s) 566, 863, 944
BshNI GGYRCC 1 cut(s) 650
BsiHKAI GWGCWC 3 cut(s) 195, 673, 730
BsiSI CCGG 2 cut(s) 115, 741
BslFI GGGAC 1 cut(s) 915
BslI CCNNNNNNNGG 2 cut(s) 244, 300
BsmAI GTCTC 1 cut(s) 45
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 915
BsmI GAATGC 4 cut(s) 295, 772, 929, 1106
BsnI GGCC 3 cut(s) 566, 863, 944
Bsp1286I GDGCHC 4 cut(s) 195, 655, 673, 730
Bsp143I GATC 3 cut(s) 6, 117, 735
BspACI CCGC 9 cut(s) 20, 23, 63, 65, 68, 149, 174, 433, 1113
BspANI GGCC 3 cut(s) 566, 863, 944
BspCNI CTCAG 3 cut(s) 55, 277, 567
BspFNI CGCG 1 cut(s) 65
BspLI GGNNCC 5 cut(s) 527, 565, 652, 862, 903
BspMAI CTGCAG 1 cut(s) 156
BspMI ACCTGC 1 cut(s) 737
BspOI GCTAGC 1 cut(s) 543
BspPI GGATC 2 cut(s) 125, 743
BspT107I GGYRCC 1 cut(s) 650
BsrDI GCAATG 2 cut(s) 235, 342
BsrI ACTGG 2 cut(s) 1034, 1035
BssECI CCNNGG 3 cut(s) 63, 864, 961
BssMI GATC 3 cut(s) 6, 117, 735
BssT1I CCWWGG 1 cut(s) 961
Bst2UI CCWGG 2 cut(s) 197, 675
Bst4CI ACNGT 5 cut(s) 76, 499, 876, 937, 1049
Bst6I CTCTTC 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 599
BstC8I GCNNGC 8 cut(s) 58, 235, 254, 258, 541, 801, 896, 946
BstDEI CTNAG 6 cut(s) 42, 285, 374, 479, 575, 1079
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 5 cut(s) 336, 501, 622, 853, 874
BstFNI CGCG 1 cut(s) 65
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 3 cut(s) 62, 167, 372
BstKTI GATC 3 cut(s) 9, 120, 738
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 3 cut(s) 6, 117, 735
BstMWI GCNNNNNNNGC 9 cut(s) 62, 171, 234, 249, 290, 659, 668, 860, 1110
BstNI CCWGG 2 cut(s) 197, 675
BstNSI RCATGY 2 cut(s) 260, 898
BstSCI CCNGG 2 cut(s) 195, 673
BstSFI CTRYAG 3 cut(s) 121, 152, 495
BstSLI GKGCMC 2 cut(s) 195, 655
BstUI CGCG 1 cut(s) 65
BstV1I GCAGC 4 cut(s) 138, 646, 769, 815
BstX2I RGATCY 1 cut(s) 117
BstYI RGATCY 1 cut(s) 117
BsuRI GGCC 3 cut(s) 566, 863, 944
BtgI CCRYGG 1 cut(s) 63
BtrI CACGTC 1 cut(s) 33
BtsCI GGATG 5 cut(s) 336, 501, 622, 853, 874
BtsI GCAGTG 1 cut(s) 218
BtsIMutI CAGTG 2 cut(s) 218, 933
BveI ACCTGC 1 cut(s) 737
Cac8I GCNNGC 8 cut(s) 58, 235, 254, 258, 541, 801, 896, 946
CaiI CAGNNNCTG 1 cut(s) 1029
CfoI GCGC 3 cut(s) 62, 167, 372
Cfr13I GGNCC 4 cut(s) 564, 861, 902, 1032
Cfr42I CCGCGG 1 cut(s) 66
CseI GACGC 1 cut(s) 146
Csp6I GTAC 2 cut(s) 157, 872
CviAII CATG 8 cut(s) 208, 257, 350, 521, 656, 821, 895, 1052
CviQI GTAC 2 cut(s) 157, 872
DdeI CTNAG 6 cut(s) 42, 285, 374, 479, 575, 1079
DpnI GATC 3 cut(s) 8, 119, 737
DpnII GATC 3 cut(s) 6, 117, 735
EaeI YGGCCR 1 cut(s) 942
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Ecl136II GAGCTC 1 cut(s) 671
Eco130I CCWWGG 1 cut(s) 961
Eco24I GRGCYC 1 cut(s) 673
Eco47I GGWCC 2 cut(s) 902, 1032
Eco53kI GAGCTC 1 cut(s) 671
Eco57I CTGAAG 1 cut(s) 571
EcoICRI GAGCTC 1 cut(s) 671
EcoO109I RGGNCCY 1 cut(s) 902
EcoRII CCWGG 2 cut(s) 195, 673
EcoT14I CCWWGG 1 cut(s) 961
EcoT22I ATGCAT 1 cut(s) 931
EcoT38I GRGCYC 1 cut(s) 673
ErhI CCWWGG 1 cut(s) 961
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 8 cut(s) 211, 260, 353, 524, 659, 824, 898, 1055
FalI AAGNNNNNCTT 2 cut(s) 292, 324
FaqI GGGAC 1 cut(s) 915
FatI CATG 8 cut(s) 207, 256, 349, 520, 655, 820, 894, 1051
FauI CCCGC 2 cut(s) 27, 156
Fnu4HI GCNGC 7 cut(s) 23, 63, 66, 152, 660, 783, 804
FokI GGATG 5 cut(s) 343, 488, 629, 860, 881
FriOI GRGCYC 1 cut(s) 673
Fsp4HI GCNGC 7 cut(s) 23, 63, 66, 152, 660, 783, 804
FspBI CTAG 1 cut(s) 540
GlaI GCGC 3 cut(s) 61, 166, 371
GluI GCNGC 7 cut(s) 23, 63, 66, 152, 660, 783, 804
GsuI CTGGAG 1 cut(s) 744
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 3 cut(s) 566, 863, 944
HapII CCGG 2 cut(s) 115, 741
HgaI GACGC 1 cut(s) 146
HhaI GCGC 3 cut(s) 62, 167, 372
Hin1II CATG 8 cut(s) 211, 260, 353, 524, 659, 824, 898, 1055
Hin6I GCGC 3 cut(s) 60, 165, 370
HinP1I GCGC 3 cut(s) 60, 165, 370
HincII GTYRAC 1 cut(s) 1045
HindII GTYRAC 1 cut(s) 1045
HinfI GANTC 1 cut(s) 1065
HpaII CCGG 2 cut(s) 115, 741
HphI GGTGA 3 cut(s) 82, 470, 713
Hpy166II GTNNAC 3 cut(s) 30, 193, 1045
Hpy188I TCNGA 3 cut(s) 576, 999, 1133
Hpy188III TCNNGA 4 cut(s) 44, 314, 812, 845
Hpy8I GTNNAC 3 cut(s) 30, 193, 1045
Hpy99I CGWCG 4 cut(s) 37, 40, 89, 140
HpyAV CCTTC 3 cut(s) 277, 374, 629
HpyCH4III ACNGT 5 cut(s) 76, 499, 876, 937, 1049
HpyCH4IV ACGT 2 cut(s) 32, 598
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 171, 234, 249, 290, 659, 668, 860, 1110
HpyF3I CTNAG 6 cut(s) 42, 285, 374, 479, 575, 1079
HpySE526I ACGT 2 cut(s) 32, 598
Hsp92II CATG 8 cut(s) 211, 260, 353, 524, 659, 824, 898, 1055
HspAI GCGC 3 cut(s) 60, 165, 370
KspI CCGCGG 1 cut(s) 66
Kzo9I GATC 3 cut(s) 6, 117, 735
LmnI GCTCC 4 cut(s) 676, 725, 743, 826
Lsp1109I GCAGC 4 cut(s) 138, 646, 769, 815
LweI GCATC 5 cut(s) 189, 607, 709, 786, 841
MaeI CTAG 1 cut(s) 540
MaeII ACGT 2 cut(s) 32, 598
MaeIII GTNAC 4 cut(s) 70, 1088, 1106, 1115
MalI GATC 3 cut(s) 8, 119, 737
MboI GATC 3 cut(s) 6, 117, 735
MboII GAAGA 4 cut(s) 107, 641, 728, 1109
MflI RGATCY 1 cut(s) 117
MhlI GDGCHC 4 cut(s) 195, 655, 673, 730
MlsI TGGCCA 1 cut(s) 944
MluCI AATT 1 cut(s) 359
MluNI TGGCCA 1 cut(s) 944
MmeI TCCRAC 1 cut(s) 977
Mox20I TGGCCA 1 cut(s) 944
Mph1103I ATGCAT 1 cut(s) 931
MscI TGGCCA 1 cut(s) 944
MseI TTAA 1 cut(s) 219
MslI CAYNNNNRTG 2 cut(s) 228, 597
Msp20I TGGCCA 1 cut(s) 944
MspA1I CMGCKG 3 cut(s) 65, 151, 1113
MspI CCGG 2 cut(s) 115, 741
MspR9I CCNGG 2 cut(s) 197, 675
Mva1269I GAATGC 4 cut(s) 295, 772, 929, 1106
MvaI CCWGG 2 cut(s) 197, 675
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 9 cut(s) 62, 171, 234, 249, 290, 659, 668, 860, 1110
NdeII GATC 3 cut(s) 6, 117, 735
NheI GCTAGC 1 cut(s) 539
NlaIII CATG 8 cut(s) 211, 260, 353, 524, 659, 824, 898, 1055
NlaIV GGNNCC 5 cut(s) 527, 565, 652, 862, 903
NmeAIII GCCGAG 1 cut(s) 889
NmuCI GTSAC 1 cut(s) 70
NsiI ATGCAT 1 cut(s) 931
NspI RCATGY 2 cut(s) 260, 898
PaeI GCATGC 2 cut(s) 260, 898
PctI GAATGC 4 cut(s) 295, 772, 929, 1106
PfeI GAWTC 1 cut(s) 1065
PfoI TCCNGGA 1 cut(s) 673
PkrI GCNGC 7 cut(s) 24, 64, 67, 153, 661, 784, 805
Ppu21I YACGTR 1 cut(s) 599
PpuMI RGGWCCY 1 cut(s) 902
Psp124BI GAGCTC 1 cut(s) 673
Psp5II RGGWCCY 1 cut(s) 902
Psp6I CCWGG 2 cut(s) 195, 673
PspGI CCWGG 2 cut(s) 195, 673
PspN4I GGNNCC 5 cut(s) 527, 565, 652, 862, 903
PspPI GGNCC 4 cut(s) 564, 861, 902, 1032
PspPPI RGGWCCY 1 cut(s) 902
PstI CTGCAG 1 cut(s) 156
PstNI CAGNNNCTG 1 cut(s) 1029
PsuI RGATCY 1 cut(s) 117
RsaI GTAC 2 cut(s) 158, 873
RsaNI GTAC 2 cut(s) 157, 872
RseI CAYNNNNRTG 2 cut(s) 228, 597
SacI GAGCTC 1 cut(s) 673
SacII CCGCGG 1 cut(s) 66
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 7 cut(s) 23, 63, 66, 152, 660, 783, 804
Sau3AI GATC 3 cut(s) 6, 117, 735
Sau96I GGNCC 4 cut(s) 564, 861, 902, 1032
ScrFI CCNGG 2 cut(s) 197, 675
SduI GDGCHC 4 cut(s) 195, 655, 673, 730
SfaNI GCATC 5 cut(s) 189, 607, 709, 786, 841
SfcI CTRYAG 3 cut(s) 121, 152, 495
Sfr303I CCGCGG 1 cut(s) 66
SgrBI CCGCGG 1 cut(s) 66
SinI GGWCC 2 cut(s) 902, 1032
SmiMI CAYNNNNRTG 2 cut(s) 228, 597
SphI GCATGC 2 cut(s) 260, 898
Sse9I AATT 1 cut(s) 359
SsiI CCGC 9 cut(s) 20, 23, 63, 65, 68, 149, 174, 433, 1113
SspMI CTAG 1 cut(s) 540
SstI GAGCTC 1 cut(s) 673
StyD4I CCNGG 2 cut(s) 195, 673
StyI CCWWGG 1 cut(s) 961
TaaI ACNGT 5 cut(s) 76, 499, 876, 937, 1049
TaiI ACGT 2 cut(s) 35, 601
TasI AATT 1 cut(s) 359
TauI GCSGC 3 cut(s) 25, 65, 68
TfiI GAWTC 1 cut(s) 1065
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 2 cut(s) 218, 940
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 4 cut(s) 151, 659, 782, 803
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 3 cut(s) 353, 648, 902
TspRI CASTG 2 cut(s) 218, 940
VneI GTGCAC 1 cut(s) 191
VpaK11BI GGWCC 2 cut(s) 902, 1032
XceI RCATGY 2 cut(s) 260, 898
XspI CTAG 1 cut(s) 540
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.