pycom10g08050
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009649
Physical Location & Seq
Reverse (-)
10308186 .. 10308804
619 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 411 bp
ATGGTGGGGCGGTACCTTTTGCTTCCTTGGCTAATTGTGCCTTTTATTCATGCTTTGAAGAGCTCAAATGAAGAGCATTTCAATGCAGCACACAATGTGATGCGCCTTTCAGCACTTCGAACGATTGTTAGTCTAAAGAATTGGGGTATTTTGAGCAAACCAATACAAGAGGAGATGAAGATGGAAATTGCTTATATCAATGCGTGTTCGATATTGCATAATGGTTTGTTGAGGAGGGAGGACTTTTTTGCAATGTATGATGGGATGGATGTTTGCTCCTTGTATGATCAGAGCTCTCAATATTTTAGGGATACTAGTTTGGAGGAGAATTTGATTGATGCTGCTTTTGCTTTTATGTTTCTAAACAAAATTGTTGGGAATTTTTACAATAAAATAAGGAATTCAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

137

Amino Acids

15.8

Weight (kDa)

6.4

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 5 - 74 2.3e-06 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 12
AccB1I GGYRCC 1 cut(s) 12
AciI CCGC 1 cut(s) 10
AcsI RAATTY 3 cut(s) 328, 379, 400
AdeI CACNNNGTG 1 cut(s) 97
AfaI GTAC 1 cut(s) 14
AgsI TTSAA 3 cut(s) 58, 82, 405
AhlI ACTAGT 1 cut(s) 314
AluBI AGCT 2 cut(s) 63, 294
AluI AGCT 2 cut(s) 63, 294
Alw21I GWGCWC 2 cut(s) 65, 296
ApeKI GCWGC 2 cut(s) 86, 341
ApoI RAATTY 3 cut(s) 328, 379, 400
Asp718I GGTACC 1 cut(s) 12
AspLEI GCGC 1 cut(s) 105
AsuII TTCGAA 1 cut(s) 118
BanI GGYRCC 1 cut(s) 12
BanII GRGCYC 2 cut(s) 65, 296
Bbv12I GWGCWC 2 cut(s) 65, 296
BbvI GCAGC 2 cut(s) 98, 328
BccI CCATC 3 cut(s) 175, 254, 259
BciVI GTATCC 1 cut(s) 304
BclI TGATCA 1 cut(s) 286
BcuI ACTAGT 1 cut(s) 314
BfaI CTAG 1 cut(s) 315
BfuI GTATCC 1 cut(s) 304
BisI GCNGC 2 cut(s) 87, 342
BlsI GCNGC 2 cut(s) 88, 343
BmiI GGNNCC 1 cut(s) 14
BmsI GCATC 2 cut(s) 90, 328
Bpu14I TTCGAA 1 cut(s) 118
BsaJI CCNNGG 1 cut(s) 26
Bse3DI GCAATG 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 26
BseGI GGATG 2 cut(s) 270, 274
BseMI GCAATG 1 cut(s) 258
BseRI GAGGAG 3 cut(s) 185, 247, 338
BseXI GCAGC 2 cut(s) 98, 328
BshNI GGYRCC 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 65, 296
Bsp119I TTCGAA 1 cut(s) 118
Bsp1286I GDGCHC 2 cut(s) 65, 296
Bsp143I GATC 1 cut(s) 286
BspACI CCGC 1 cut(s) 10
BspLI GGNNCC 1 cut(s) 14
BspQI GCTCTTC 2 cut(s) 53, 66
BspT104I TTCGAA 1 cut(s) 118
BspT107I GGYRCC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 258
BssECI CCNNGG 1 cut(s) 26
BssMI GATC 1 cut(s) 286
BssT1I CCWWGG 1 cut(s) 26
Bst6I CTCTTC 2 cut(s) 53, 66
BstBI TTCGAA 1 cut(s) 118
BstF5I GGATG 2 cut(s) 270, 274
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 1 cut(s) 289
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 3 cut(s) 28, 37, 347
BstV1I GCAGC 2 cut(s) 98, 328
BsuI GTATCC 1 cut(s) 304
BtsCI GGATG 2 cut(s) 270, 274
CfoI GCGC 1 cut(s) 105
Csp6I GTAC 1 cut(s) 13
CviAII CATG 1 cut(s) 50
CviJI RGCY 3 cut(s) 31, 63, 294
CviKI_1 RGCY 3 cut(s) 31, 63, 294
CviQI GTAC 1 cut(s) 13
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
DraIII CACNNNGTG 1 cut(s) 97
Eam1104I CTCTTC 2 cut(s) 53, 66
EarI CTCTTC 2 cut(s) 53, 66
Ecl136II GAGCTC 2 cut(s) 63, 294
Eco130I CCWWGG 1 cut(s) 26
Eco24I GRGCYC 2 cut(s) 65, 296
Eco53kI GAGCTC 2 cut(s) 63, 294
EcoICRI GAGCTC 2 cut(s) 63, 294
EcoRI GAATTC 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 26
EcoT38I GRGCYC 2 cut(s) 65, 296
ErhI CCWWGG 1 cut(s) 26
FaeI CATG 1 cut(s) 53
FaiI YATR 7 cut(s) 51, 195, 219, 258, 285, 356, 409
FatI CATG 1 cut(s) 49
FbaI TGATCA 1 cut(s) 286
Fnu4HI GCNGC 2 cut(s) 87, 342
FokI GGATG 2 cut(s) 277, 281
FriOI GRGCYC 2 cut(s) 65, 296
Fsp4HI GCNGC 2 cut(s) 87, 342
FspBI CTAG 1 cut(s) 315
GlaI GCGC 1 cut(s) 104
GluI GCNGC 2 cut(s) 87, 342
HhaI GCGC 1 cut(s) 105
Hin1II CATG 1 cut(s) 53
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
Hpy188I TCNGA 1 cut(s) 291
HpyCH4V TGCA 3 cut(s) 86, 217, 251
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 37, 347
Hsp92II CATG 1 cut(s) 53
HspAI GCGC 1 cut(s) 103
KpnI GGTACC 1 cut(s) 16
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 1 cut(s) 286
LguI GCTCTTC 2 cut(s) 53, 66
LmnI GCTCC 1 cut(s) 281
Lsp1109I GCAGC 2 cut(s) 98, 328
LweI GCATC 2 cut(s) 90, 328
MaeI CTAG 1 cut(s) 315
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 3 cut(s) 70, 83, 190
MhlI GDGCHC 2 cut(s) 65, 296
MluCI AATT 7 cut(s) 33, 139, 186, 328, 369, 379, 400
MnlI CCTC 5 cut(s) 163, 225, 228, 232, 316
MslI CAYNNNNRTG 1 cut(s) 81
MwoI GCNNNNNNNGC 3 cut(s) 28, 37, 347
NdeII GATC 1 cut(s) 286
NlaIII CATG 1 cut(s) 53
NlaIV GGNNCC 1 cut(s) 14
NspV TTCGAA 1 cut(s) 118
PciSI GCTCTTC 2 cut(s) 53, 66
PkrI GCNGC 2 cut(s) 88, 343
Psp124BI GAGCTC 2 cut(s) 65, 296
PspN4I GGNNCC 1 cut(s) 14
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
RseI CAYNNNNRTG 1 cut(s) 81
SacI GAGCTC 2 cut(s) 65, 296
SapI GCTCTTC 2 cut(s) 53, 66
SatI GCNGC 2 cut(s) 87, 342
Sau3AI GATC 1 cut(s) 286
SduI GDGCHC 2 cut(s) 65, 296
SetI ASST 3 cut(s) 18, 65, 296
SfaNI GCATC 2 cut(s) 90, 328
SfuI TTCGAA 1 cut(s) 118
SgeI CNNG 6 cut(s) 39, 62, 179, 216, 292, 327
SmiMI CAYNNNNRTG 1 cut(s) 81
SpeI ACTAGT 1 cut(s) 314
Sse9I AATT 7 cut(s) 33, 139, 186, 328, 369, 379, 400
SsiI CCGC 1 cut(s) 10
SspI AATATT 1 cut(s) 302
SspMI CTAG 1 cut(s) 315
SstI GAGCTC 2 cut(s) 65, 296
StyI CCWWGG 1 cut(s) 26
TaqI TCGA 2 cut(s) 118, 209
TasI AATT 7 cut(s) 33, 139, 186, 328, 369, 379, 400
TseI GCWGC 2 cut(s) 86, 341
TspDTI ATGAA 3 cut(s) 38, 84, 191
XapI RAATTY 3 cut(s) 328, 379, 400
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.