pycom10g11800
ERF Family

Histidine phosphatase superfamily (branch 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003894
Physical Location & Seq
Forward (+)
15375156 .. 15377053
1898 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
ATGTACAATAAAATCACATACCGTACGTGGAGGGTGTGTGAAGAGGAGAAGGAGAGGAGTGCGGTGGCGGGGGGCGGTGTAGAGATGGCGGAGTTTCTGAGGAACAGATACTGGGTGCTGAGGCACGGGAAGAGCATTCCGAATGAGAGAGGCATCATTGTTTCTTCCATGGAAAATGGTACCCGCCCAGAATATCAATTGGCGCCAGAAGGTGTAAGTCAGGCACAATCAGCTGGACAATCATTCCAAAAGGTACTTGAGGAAAACAACGTACCGCTTGATAATGTTCGGATTTGCTACTCTCCGTTTTCAAGAACAAGTCAGACTGCTCAAGTTGTTGCATCTGTCCTGAACATTCCATTTGAGGGGGCTCAATGCAAGGTGATGGAAAATCTTCGTGAACGCTTCTTTGGTCCTTCGTATGAGCTTTTGTCACATGATAAATATCACGACATATGGGCTCTTGATGAGAAAGATCCACGCATGAGACCGGCAGAGGGAGGAGTGAGTGTCGATGATGTTGCGTGCAGACTTGCAGAAGCAATGGAAACTATGGAGTCACAATTTCAAGGGTGCACGATCTTAGTTGTTAGTCACGGCGATACCCTACAAATCTTGCAGACGATACTCAATGCTGCCAAGCTAAACGCGGGATCCAGTTACACTGACCTGTCATCAAGAATTCAAGCAGTTCGAACCCCTCCCATCTTATCACAGCACCGGAAGTTTTCCCTGCTTACAGCAGAGCTCCGGGCAGTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

255

Amino Acids

28.53

Weight (kDa)

6.01

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 38 - 216 3.3e-21 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 179
AccB1I GGYRCC 2 cut(s) 179, 202
AccII CGCG 1 cut(s) 650
AciI CCGC 7 cut(s) 62, 68, 75, 89, 184, 275, 650
AclWI GGATC 3 cut(s) 470, 648, 661
AcsI RAATTY 1 cut(s) 681
AcyI GRCGYC 1 cut(s) 203
AfaI GTAC 5 cut(s) 5, 25, 181, 255, 273
AfiI CCNNNNNNNGG 2 cut(s) 365, 497
AgsI TTSAA 3 cut(s) 312, 569, 686
AjuI GAANNNNNNNTTGG 2 cut(s) 393, 425
AluBI AGCT 4 cut(s) 233, 427, 643, 748
AluI AGCT 4 cut(s) 233, 427, 643, 748
Alw21I GWGCWC 2 cut(s) 578, 750
Alw26I GTCTC 1 cut(s) 481
Alw44I GTGCAC 1 cut(s) 574
AlwI GGATC 3 cut(s) 470, 648, 661
AlwNI CAGNNNCTG 1 cut(s) 111
ApaLI GTGCAC 1 cut(s) 574
ApeKI GCWGC 1 cut(s) 635
ApoI RAATTY 1 cut(s) 681
Asp700I GAANNNNTTC 1 cut(s) 393
Asp718I GGTACC 1 cut(s) 179
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 1 cut(s) 413
AsuC2I CCSGG 1 cut(s) 752
AsuHPI GGTGA 1 cut(s) 394
AsuII TTCGAA 1 cut(s) 694
AvaII GGWCC 1 cut(s) 413
BaeGI GKGCMC 1 cut(s) 578
BamHI GGATCC 1 cut(s) 653
BanI GGYRCC 2 cut(s) 179, 202
BanII GRGCYC 3 cut(s) 373, 463, 750
Bbv12I GWGCWC 2 cut(s) 578, 750
BbvCI CCTCAGC 1 cut(s) 119
BbvI GCAGC 1 cut(s) 622
BccI CCATC 3 cut(s) 79, 379, 713
BceAI ACGGC 1 cut(s) 613
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BcnI CCSGG 1 cut(s) 752
BcoDI GTCTC 1 cut(s) 481
BfoI RGCGCY 1 cut(s) 206
BisI GCNGC 1 cut(s) 636
BlsI GCNGC 1 cut(s) 637
Bme1390I CCNGG 1 cut(s) 752
Bme18I GGWCC 1 cut(s) 413
BmgT120I GGNCC 1 cut(s) 413
BmiI GGNNCC 3 cut(s) 181, 204, 655
BmrFI CCNGG 1 cut(s) 752
BmrI ACTGGG 1 cut(s) 121
BmsI GCATC 2 cut(s) 162, 350
BmuI ACTGGG 1 cut(s) 121
Bpu10I CCTNAGC 1 cut(s) 119
Bpu14I TTCGAA 1 cut(s) 694
BpuEI CTTGAG 2 cut(s) 278, 315
BpuMI CCSGG 1 cut(s) 752
BsaAI YACGTR 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 481
BsaJI CCNNGG 1 cut(s) 168
BsaWI WCCGGW 1 cut(s) 720
BsaXI ACNNNNNCTCC 4 cut(s) 22, 52, 495, 525
Bsc4I CCNNNNNNNGG 2 cut(s) 365, 497
Bse118I RCCGGY 1 cut(s) 490
Bse1I ACTGG 2 cut(s) 116, 657
Bse3DI GCAATG 1 cut(s) 549
Bse8I GATNNNNATC 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 168
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 2 cut(s) 365, 497
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 2 cut(s) 89, 110
BseNI ACTGG 2 cut(s) 116, 657
BseRI GAGGAG 3 cut(s) 59, 70, 516
BseSI GKGCMC 1 cut(s) 578
BseXI GCAGC 1 cut(s) 622
BsgI GTGCAG 1 cut(s) 547
Bsh1236I CGCG 1 cut(s) 650
BshNI GGYRCC 2 cut(s) 179, 202
BsiHKAI GWGCWC 2 cut(s) 578, 750
BsiSI CCGG 3 cut(s) 491, 721, 751
BsiWI CGTACG 1 cut(s) 23
BslI CCNNNNNNNGG 2 cut(s) 365, 497
BsmAI GTCTC 1 cut(s) 481
BsmI GAATGC 1 cut(s) 135
Bso31I GGTCTC 1 cut(s) 481
Bsp119I TTCGAA 1 cut(s) 694
Bsp1286I GDGCHC 4 cut(s) 373, 463, 578, 750
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 475, 579, 653
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 7 cut(s) 62, 68, 75, 89, 184, 275, 650
BspCNI CTCAG 2 cut(s) 90, 111
BspFNI CGCG 1 cut(s) 650
BspLI GGNNCC 3 cut(s) 181, 204, 655
BspPI GGATC 3 cut(s) 470, 648, 661
BspQI GCTCTTC 1 cut(s) 125
BspT104I TTCGAA 1 cut(s) 694
BspT107I GGYRCC 2 cut(s) 179, 202
BspTNI GGTCTC 1 cut(s) 481
BsrDI GCAATG 1 cut(s) 549
BsrFI RCCGGY 1 cut(s) 490
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 116, 657
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 3 cut(s) 475, 579, 653
BssNI GRCGYC 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 23
Bst6I CTCTTC 2 cut(s) 36, 125
BstACI GRCGYC 1 cut(s) 203
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 27
BstBI TTCGAA 1 cut(s) 694
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 3 cut(s) 98, 119, 583
BstDSI CCRYGG 1 cut(s) 168
BstFNI CGCG 1 cut(s) 650
BstH2I RGCGCY 1 cut(s) 206
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 3 cut(s) 478, 582, 656
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 3 cut(s) 475, 579, 653
BstMWI GCNNNNNNNGC 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 750
BstSLI GKGCMC 1 cut(s) 578
BstUI CGCG 1 cut(s) 650
BstV1I GCAGC 1 cut(s) 622
BstX2I RGATCY 2 cut(s) 475, 653
BstYI RGATCY 2 cut(s) 475, 653
BtgI CCRYGG 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 2 cut(s) 663, 762
Cac8I GCNNGC 1 cut(s) 526
CaiI CAGNNNCTG 1 cut(s) 111
CfoI GCGC 1 cut(s) 205
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 1 cut(s) 413
Csp6I GTAC 5 cut(s) 4, 24, 180, 254, 272
CviAII CATG 3 cut(s) 169, 437, 484
CviJI RGCY 6 cut(s) 233, 371, 427, 461, 643, 748
CviKI_1 RGCY 6 cut(s) 233, 371, 427, 461, 643, 748
CviQI GTAC 5 cut(s) 4, 24, 180, 254, 272
DdeI CTNAG 3 cut(s) 98, 119, 583
DinI GGCGCC 1 cut(s) 204
DpnI GATC 3 cut(s) 477, 581, 655
DpnII GATC 3 cut(s) 475, 579, 653
Eam1104I CTCTTC 2 cut(s) 36, 125
EarI CTCTTC 2 cut(s) 36, 125
EciI GGCGGA 1 cut(s) 104
Ecl136II GAGCTC 1 cut(s) 748
Eco130I CCWWGG 1 cut(s) 168
Eco24I GRGCYC 3 cut(s) 373, 463, 750
Eco31I GGTCTC 1 cut(s) 481
Eco47I GGWCC 1 cut(s) 413
Eco53kI GAGCTC 1 cut(s) 748
EcoICRI GAGCTC 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 681
EcoT14I CCWWGG 1 cut(s) 168
EcoT38I GRGCYC 3 cut(s) 373, 463, 750
EgeI GGCGCC 1 cut(s) 204
EheI GGCGCC 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 3 cut(s) 172, 440, 487
FaiI YATR 9 cut(s) 19, 170, 423, 438, 455, 457, 485, 554, 763
FatI CATG 3 cut(s) 168, 436, 483
FauI CCCGC 3 cut(s) 61, 191, 643
FauNDI CATATG 1 cut(s) 455
Fnu4HI GCNGC 1 cut(s) 636
FriOI GRGCYC 3 cut(s) 373, 463, 750
Fsp4HI GCNGC 1 cut(s) 636
GlaI GCGC 1 cut(s) 204
GluI GCNGC 1 cut(s) 636
HaeII RGCGCY 1 cut(s) 206
HapII CCGG 3 cut(s) 491, 721, 751
HhaI GCGC 1 cut(s) 205
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 3 cut(s) 172, 440, 487
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HinfI GANTC 1 cut(s) 557
HpaII CCGG 3 cut(s) 491, 721, 751
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 2 cut(s) 401, 576
Hpy188I TCNGA 4 cut(s) 99, 141, 291, 324
Hpy188III TCNNGA 6 cut(s) 312, 349, 398, 449, 464, 678
Hpy8I GTNNAC 2 cut(s) 401, 576
HpyAV CCTTC 3 cut(s) 43, 203, 426
HpyCH4III ACNGT 1 cut(s) 23
HpyCH4IV ACGT 2 cut(s) 26, 270
HpyCH4V TGCA 6 cut(s) 341, 378, 528, 536, 576, 619
HpyF10VI GCNNNNNNNGC 1 cut(s) 230
HpyF3I CTNAG 3 cut(s) 98, 119, 583
HpySE526I ACGT 2 cut(s) 26, 270
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 3 cut(s) 172, 440, 487
HspAI GCGC 1 cut(s) 203
KasI GGCGCC 1 cut(s) 202
KpnI GGTACC 1 cut(s) 183
Kzo9I GATC 3 cut(s) 475, 579, 653
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 1 cut(s) 753
Lsp1109I GCAGC 1 cut(s) 622
LweI GCATC 2 cut(s) 162, 350
MaeII ACGT 2 cut(s) 26, 270
MaeIII GTNAC 4 cut(s) 432, 558, 593, 659
MalI GATC 3 cut(s) 477, 581, 655
MboI GATC 3 cut(s) 475, 579, 653
MboII GAAGA 4 cut(s) 53, 142, 156, 386
MfeI CAATTG 1 cut(s) 197
MflI RGATCY 2 cut(s) 475, 653
MhlI GDGCHC 4 cut(s) 373, 463, 578, 750
MluCI AATT 3 cut(s) 197, 563, 681
Mly113I GGCGCC 1 cut(s) 203
MlyI GAGTC 1 cut(s) 566
MroXI GAANNNNTTC 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 3 cut(s) 491, 721, 751
MspR9I CCNGG 1 cut(s) 752
MunI CAATTG 1 cut(s) 197
Mva1269I GAATGC 1 cut(s) 135
MvnI CGCG 1 cut(s) 650
MwoI GCNNNNNNNGC 1 cut(s) 230
NarI GGCGCC 1 cut(s) 203
NciI CCSGG 1 cut(s) 752
NcoI CCATGG 1 cut(s) 168
NdeI CATATG 1 cut(s) 455
NdeII GATC 3 cut(s) 475, 579, 653
NlaIII CATG 3 cut(s) 172, 440, 487
NlaIV GGNNCC 3 cut(s) 181, 204, 655
NmuCI GTSAC 3 cut(s) 432, 558, 593
NspV TTCGAA 1 cut(s) 694
PciSI GCTCTTC 1 cut(s) 125
PctI GAATGC 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 393
Pfl23II CGTACG 1 cut(s) 23
PkrI GCNGC 1 cut(s) 637
PleI GAGTC 1 cut(s) 565
PluTI GGCGCC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 565
Ppu21I YACGTR 1 cut(s) 27
Psp124BI GAGCTC 1 cut(s) 750
PspLI CGTACG 1 cut(s) 23
PspN4I GGNNCC 3 cut(s) 181, 204, 655
PspPI GGNCC 1 cut(s) 413
PstNI CAGNNNCTG 1 cut(s) 111
PsuI RGATCY 2 cut(s) 475, 653
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 5 cut(s) 5, 25, 181, 255, 273
RsaNI GTAC 5 cut(s) 4, 24, 180, 254, 272
SacI GAGCTC 1 cut(s) 750
SapI GCTCTTC 1 cut(s) 125
SatI GCNGC 1 cut(s) 636
Sau3AI GATC 3 cut(s) 475, 579, 653
Sau96I GGNCC 1 cut(s) 413
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 1 cut(s) 752
SduI GDGCHC 4 cut(s) 373, 463, 578, 750
SfaNI GCATC 2 cut(s) 162, 350
SfoI GGCGCC 1 cut(s) 204
SfuI TTCGAA 1 cut(s) 694
SinI GGWCC 1 cut(s) 413
SmlI CTYRAG 2 cut(s) 257, 330
SmoI CTYRAG 2 cut(s) 257, 330
Sse9I AATT 3 cut(s) 197, 563, 681
SsiI CCGC 7 cut(s) 62, 68, 75, 89, 184, 275, 650
SspDI GGCGCC 1 cut(s) 202
SstI GAGCTC 1 cut(s) 750
StyD4I CCNGG 1 cut(s) 750
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 1 cut(s) 23
TaiI ACGT 2 cut(s) 29, 273
TaqI TCGA 2 cut(s) 513, 694
TasI AATT 3 cut(s) 197, 563, 681
TatI WGTACW 1 cut(s) 3
TscAI CASTG 2 cut(s) 670, 762
TseFI GTSAC 3 cut(s) 432, 558, 593
TseI GCWGC 1 cut(s) 635
Tsp45I GTSAC 3 cut(s) 432, 558, 593
TspGWI ACGGA 1 cut(s) 294
TspRI CASTG 2 cut(s) 670, 762
VneI GTGCAC 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 413
XapI RAATTY 1 cut(s) 681
XmnI GAANNNNTTC 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.