pycom10g25690

PAP-specific phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007801
Physical Location & Seq
Forward (+)
27227228 .. 27230204
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1236 bp
ATGATTCTCGTCCACTCAGCCCGCCATTTTACGACTGCAAGATTCACTCAGCCAGATTCGGCCCTCCCGCGGCGGCGCTTCCGCGTGAGGTCGAGTTTGCCGTTCGGTACTGAAAAGGCCAACTACTATCGAGAGCTGGAAGCTGCCGTCGATGTCGTGGAGAGAGCTTGCGCGCTGTGCGTTGACATCCAGTCGTCGGTGCTATCAACTCAACGGCGGATCGTTGAAAAGAGCGACCAGACTCCGGTGACGGTGGCGGATTTTGGAGTCCAGGCCCTCGTCAGTTTGGAGCTTGGGAAACGATTCCCTTTGATTCCATTGGTAGCGGAAGAAGACTCCGCGTTTGTACGGTCGAATAATCTGGTGGAGCCGGTGCTCAATGCGGTGCTTGACAAATCTAGTATTGGAGAGAACCCATGGACGGCTGATGAAGTATTGGAAGCCATTGACAGAGGGGGTCCAGAGGGTTTTGCTTTCGGAGCTCAGCCCGCCACTTATTGGGTACTTGACCCCATTGATGGCACAAAAGGATTTGTGGAGGGAAATCCGGCGTTATATGTGGTTGGCTTGGCACTTGTTGTTGATGGAGAGGTTGTATTGGGTGTCATGGGCTGCCCGAACTGGAAGAATGATATATCGAACAAATCCGCCTCTGAAGTCCAGGAAGAAAAAAGCAGTCCACCTGGATCAGGGATTATTATGGTTGCACATACAGGCTGTGGCACGTGGACAAAACGTTTGTCATCTGTTGTAAATTCCACAGCCAAAATGCCTTATTCATGGACACGATGCTTTGTTGATGGGTCTTGTATCGTGGAAGAGGCACGATTTTCTATCACAGATAGTGATGTATGGGAGTCATTTCCGCTATCATCTTTATTCAGCTCCACAACTAATGCTGATTGCATTGATGAGTGCGAAATTCTTCTTGTAAAATCTTGTTGTGGAAGTTTATCCAAGTATATAATGGTGGCTTCAGGTGTGGCATCTCTTCATATGCAGCGTGTGAAAGTTCATAAAATTATCAAGGCTTGGGATCACGCTGTTGGTGTTATATGTGTGCATGAAGCGGGGGGAAAGGTGACTGACTGGAAGGGAGATGAACTTAATCTAGCAGCAGATGAAGTTGGACGGAGGAATATATACCCTTCCGGTGGAATCCTTGTGACTAATAGCACCTTGCATAACCGGCTTGTCGAGATGATATCTTCCAGTTCGTCCACTGTTTCGCGGTGA

Protein Analysis

412

Amino Acids

44.62

Weight (kDa)

5.4

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inositol_P PF00459 61 - 403 5.6e-29 Inositol monophosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 498
AccII CGCG 5 cut(s) 70, 84, 173, 341, 1231
AclI AACGTT 1 cut(s) 736
AclWI GGATC 3 cut(s) 227, 694, 1044
AcsI RAATTY 2 cut(s) 754, 921
AcuI CTGAAG 2 cut(s) 675, 960
AcvI CACGTG 1 cut(s) 726
AfaI GTAC 3 cut(s) 109, 348, 504
AfiI CCNNNNNNNGG 8 cut(s) 69, 196, 244, 421, 498, 518, 689, 1154
AgsI TTSAA 1 cut(s) 227
AhdI GACNNNNNGTC 1 cut(s) 190
AjnI CCWGG 3 cut(s) 270, 660, 682
AluBI AGCT 6 cut(s) 136, 143, 167, 292, 482, 885
AluI AGCT 6 cut(s) 136, 143, 167, 292, 482, 885
Alw21I GWGCWC 2 cut(s) 378, 484
AlwI GGATC 3 cut(s) 227, 694, 1044
AoxI GGCC 3 cut(s) 60, 117, 273
ApeKI GCWGC 4 cut(s) 143, 612, 1000, 1115
ApoI RAATTY 2 cut(s) 754, 921
ArsI GACNNNNNNTTYG 6 cut(s) 326, 358, 721, 725, 753, 757
Asp700I GAANNNNTTC 2 cut(s) 302, 924
AspLEI GCGC 3 cut(s) 78, 173, 175
AspS9I GGNCC 3 cut(s) 61, 274, 458
AsuHPI GGTGA 2 cut(s) 259, 1093
AvaII GGWCC 1 cut(s) 458
BanII GRGCYC 1 cut(s) 484
BbrPI CACGTG 1 cut(s) 726
BbsI GAAGAC 1 cut(s) 339
Bbv12I GWGCWC 2 cut(s) 378, 484
BbvI GCAGC 4 cut(s) 130, 599, 1012, 1127
BccI CCATC 3 cut(s) 512, 578, 794
BceAI ACGGC 4 cut(s) 85, 131, 230, 438
BciT130I CCWGG 3 cut(s) 272, 662, 684
BfaI CTAG 2 cut(s) 399, 1112
BfoI RGCGCY 1 cut(s) 79
BisI GCNGC 6 cut(s) 71, 74, 144, 613, 1001, 1116
BlpI GCTNAGC 1 cut(s) 483
BlsI GCNGC 6 cut(s) 72, 75, 145, 614, 1002, 1117
Bme1390I CCNGG 3 cut(s) 272, 662, 684
Bme18I GGWCC 1 cut(s) 458
BmeRI GACNNNNNGTC 1 cut(s) 190
BmgT120I GGNCC 3 cut(s) 61, 274, 458
BmiI GGNNCC 2 cut(s) 369, 459
BmrFI CCNGG 3 cut(s) 272, 662, 684
BmsI GCATC 2 cut(s) 779, 995
BpiI GAAGAC 1 cut(s) 339
Bpu1102I GCTNAGC 1 cut(s) 483
BsaAI YACGTR 1 cut(s) 726
BsaJI CCNNGG 2 cut(s) 68, 416
BsaWI WCCGGW 2 cut(s) 244, 1151
BsaXI ACNNNNNCTCC 2 cut(s) 579, 609
Bsc4I CCNNNNNNNGG 8 cut(s) 69, 196, 244, 421, 498, 518, 689, 1154
Bse118I RCCGGY 2 cut(s) 370, 1188
Bse1I ACTGG 4 cut(s) 190, 626, 1094, 1212
BseBI CCWGG 3 cut(s) 272, 662, 684
BseDI CCNNGG 2 cut(s) 68, 416
BseGI GGATG 1 cut(s) 186
BseLI CCNNNNNNNGG 8 cut(s) 69, 196, 244, 421, 498, 518, 689, 1154
BseMII CTCAG 3 cut(s) 30, 62, 497
BseNI ACTGG 4 cut(s) 190, 626, 1094, 1212
BsePI GCGCGC 1 cut(s) 171
BseXI GCAGC 4 cut(s) 130, 599, 1012, 1127
Bsh1236I CGCG 5 cut(s) 70, 84, 173, 341, 1231
Bsh1285I CGRYCG 1 cut(s) 353
BshFI GGCC 3 cut(s) 62, 119, 275
BsiEI CGRYCG 1 cut(s) 353
BsiHKAI GWGCWC 2 cut(s) 378, 484
BsiSI CCGG 5 cut(s) 245, 371, 548, 1152, 1189
BslI CCNNNNNNNGG 8 cut(s) 69, 196, 244, 421, 498, 518, 689, 1154
BsnI GGCC 3 cut(s) 62, 119, 275
Bsp1286I GDGCHC 2 cut(s) 378, 484
Bsp143I GATC 3 cut(s) 219, 686, 1036
Bsp1720I GCTNAGC 1 cut(s) 483
Bsp19I CCATGG 1 cut(s) 416
BspANI GGCC 3 cut(s) 62, 119, 275
BspCNI CTCAG 3 cut(s) 29, 61, 496
BspFNI CGCG 5 cut(s) 70, 84, 173, 341, 1231
BspLI GGNNCC 2 cut(s) 369, 459
BspPI GGATC 3 cut(s) 227, 694, 1044
BsrFI RCCGGY 2 cut(s) 370, 1188
BsrI ACTGG 4 cut(s) 190, 626, 1094, 1212
BssAI RCCGGY 2 cut(s) 370, 1188
BssECI CCNNGG 2 cut(s) 68, 416
BssHII GCGCGC 1 cut(s) 171
BssMI GATC 3 cut(s) 219, 686, 1036
BssT1I CCWWGG 1 cut(s) 416
Bst2UI CCWGG 3 cut(s) 272, 662, 684
Bst4CI ACNGT 3 cut(s) 253, 351, 1225
Bst6I CTCTTC 2 cut(s) 813, 996
BstBAI YACGTR 1 cut(s) 726
BstC8I GCNNGC 4 cut(s) 22, 169, 173, 489
BstDEI CTNAG 3 cut(s) 16, 48, 483
BstDSI CCRYGG 2 cut(s) 68, 416
BstENI CCTNNNNNAGG 1 cut(s) 687
BstF5I GGATG 1 cut(s) 186
BstFNI CGCG 5 cut(s) 70, 84, 173, 341, 1231
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 3 cut(s) 78, 173, 175
BstKTI GATC 3 cut(s) 222, 689, 1039
BstMBI GATC 3 cut(s) 219, 686, 1036
BstMCI CGRYCG 1 cut(s) 353
BstMWI GCNNNNNNNGC 4 cut(s) 177, 479, 488, 1189
BstNI CCWGG 3 cut(s) 272, 662, 684
BstSCI CCNGG 3 cut(s) 270, 660, 682
BstUI CGCG 5 cut(s) 70, 84, 173, 341, 1231
BstV1I GCAGC 4 cut(s) 130, 599, 1012, 1127
BstV2I GAAGAC 1 cut(s) 339
BsuRI GGCC 3 cut(s) 62, 119, 275
BtgI CCRYGG 2 cut(s) 68, 416
BtsCI GGATG 1 cut(s) 186
BtsIMutI CAGTG 1 cut(s) 1221
Cac8I GCNNGC 4 cut(s) 22, 169, 173, 489
CfoI GCGC 3 cut(s) 78, 173, 175
Cfr10I RCCGGY 2 cut(s) 370, 1188
Cfr13I GGNCC 3 cut(s) 61, 274, 458
Cfr42I CCGCGG 1 cut(s) 71
Csp6I GTAC 3 cut(s) 108, 347, 503
CviAII CATG 4 cut(s) 417, 607, 780, 1064
CviQI GTAC 3 cut(s) 108, 347, 503
DdeI CTNAG 3 cut(s) 16, 48, 483
DpnI GATC 3 cut(s) 221, 688, 1038
DpnII GATC 3 cut(s) 219, 686, 1036
DriI GACNNNNNGTC 1 cut(s) 190
Eam1104I CTCTTC 2 cut(s) 813, 996
Eam1105I GACNNNNNGTC 1 cut(s) 190
EarI CTCTTC 2 cut(s) 813, 996
EciI GGCGGA 3 cut(s) 232, 272, 637
Ecl136II GAGCTC 1 cut(s) 482
Eco130I CCWWGG 1 cut(s) 416
Eco24I GRGCYC 1 cut(s) 484
Eco32I GATATC 1 cut(s) 1206
Eco47I GGWCC 1 cut(s) 458
Eco53kI GAGCTC 1 cut(s) 482
Eco57I CTGAAG 2 cut(s) 675, 960
Eco72I CACGTG 1 cut(s) 726
EcoICRI GAGCTC 1 cut(s) 482
EcoNI CCTNNNNNAGG 1 cut(s) 687
EcoO109I RGGNCCY 1 cut(s) 274
EcoRII CCWGG 3 cut(s) 270, 660, 682
EcoRV GATATC 1 cut(s) 1206
EcoT14I CCWWGG 1 cut(s) 416
EcoT38I GRGCYC 1 cut(s) 484
ErhI CCWWGG 1 cut(s) 416
FaeI CATG 4 cut(s) 420, 610, 783, 1067
FatI CATG 4 cut(s) 416, 606, 779, 1063
FauI CCCGC 4 cut(s) 29, 75, 496, 1063
FauNDI CATATG 1 cut(s) 996
Fnu4HI GCNGC 6 cut(s) 71, 74, 144, 613, 1001, 1116
FokI GGATG 1 cut(s) 173
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 6 cut(s) 71, 74, 144, 613, 1001, 1116
FspBI CTAG 2 cut(s) 399, 1112
GlaI GCGC 3 cut(s) 77, 172, 174
GluI GCNGC 6 cut(s) 71, 74, 144, 613, 1001, 1116
HaeII RGCGCY 1 cut(s) 79
HaeIII GGCC 3 cut(s) 62, 119, 275
HapII CCGG 5 cut(s) 245, 371, 548, 1152, 1189
HhaI GCGC 3 cut(s) 78, 173, 175
Hin1II CATG 4 cut(s) 420, 610, 783, 1067
Hin6I GCGC 3 cut(s) 76, 171, 173
HinP1I GCGC 3 cut(s) 76, 171, 173
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HpaII CCGG 5 cut(s) 245, 371, 548, 1152, 1189
HphI GGTGA 2 cut(s) 259, 1093
Hpy166II GTNNAC 5 cut(s) 13, 184, 680, 729, 1221
Hpy188I TCNGA 2 cut(s) 479, 655
Hpy188III TCNNGA 3 cut(s) 131, 461, 1198
Hpy8I GTNNAC 5 cut(s) 13, 184, 680, 729, 1221
Hpy99I CGWCG 2 cut(s) 152, 199
HpyAV CCTTC 2 cut(s) 1087, 1158
HpyCH4III ACNGT 3 cut(s) 253, 351, 1225
HpyCH4IV ACGT 2 cut(s) 725, 736
HpyCH4V TGCA 6 cut(s) 38, 707, 906, 1000, 1063, 1183
HpyF10VI GCNNNNNNNGC 4 cut(s) 177, 479, 488, 1189
HpyF3I CTNAG 3 cut(s) 16, 48, 483
HpySE526I ACGT 2 cut(s) 725, 736
Hsp92II CATG 4 cut(s) 420, 610, 783, 1067
HspAI GCGC 3 cut(s) 76, 171, 173
KspI CCGCGG 1 cut(s) 71
Kzo9I GATC 3 cut(s) 219, 686, 1036
LmnI GCTCC 4 cut(s) 289, 367, 479, 890
Lsp1109I GCAGC 4 cut(s) 130, 599, 1012, 1127
LweI GCATC 2 cut(s) 779, 995
MaeI CTAG 2 cut(s) 399, 1112
MaeII ACGT 2 cut(s) 725, 736
MaeIII GTNAC 3 cut(s) 247, 1081, 1165
MalI GATC 3 cut(s) 221, 688, 1038
MboI GATC 3 cut(s) 219, 686, 1036
MboII GAAGA 8 cut(s) 341, 344, 637, 677, 830, 917, 983, 1200
MhlI GDGCHC 2 cut(s) 378, 484
MluCI AATT 3 cut(s) 754, 921, 1020
MlyI GAGTC 4 cut(s) 235, 276, 329, 866
MmeI TCCRAC 1 cut(s) 1108
MroXI GAANNNNTTC 2 cut(s) 302, 924
MseI TTAA 1 cut(s) 1107
MspA1I CMGCKG 1 cut(s) 70
MspI CCGG 5 cut(s) 245, 371, 548, 1152, 1189
MspR9I CCNGG 3 cut(s) 272, 662, 684
MvaI CCWGG 3 cut(s) 272, 662, 684
MvnI CGCG 5 cut(s) 70, 84, 173, 341, 1231
MwoI GCNNNNNNNGC 4 cut(s) 177, 479, 488, 1189
NcoI CCATGG 1 cut(s) 416
NdeI CATATG 1 cut(s) 996
NdeII GATC 3 cut(s) 219, 686, 1036
NlaIII CATG 4 cut(s) 420, 610, 783, 1067
NlaIV GGNNCC 2 cut(s) 369, 459
NmuCI GTSAC 3 cut(s) 247, 1081, 1165
PauI GCGCGC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 98
PdmI GAANNNNTTC 2 cut(s) 302, 924
PfeI GAWTC 6 cut(s) 4, 42, 56, 303, 313, 1158
PflMI CCANNNNNTGG 1 cut(s) 498
PfoI TCCNGGA 1 cut(s) 660
PkrI GCNGC 6 cut(s) 72, 75, 145, 614, 1002, 1117
PleI GAGTC 4 cut(s) 235, 275, 329, 865
PmaCI CACGTG 1 cut(s) 726
PmlI CACGTG 1 cut(s) 726
PpsI GAGTC 4 cut(s) 235, 275, 329, 865
Ppu21I YACGTR 1 cut(s) 726
Psp124BI GAGCTC 1 cut(s) 484
Psp1406I AACGTT 1 cut(s) 736
Psp6I CCWGG 3 cut(s) 270, 660, 682
PspCI CACGTG 1 cut(s) 726
PspGI CCWGG 3 cut(s) 270, 660, 682
PspN4I GGNNCC 2 cut(s) 369, 459
PspPI GGNCC 3 cut(s) 61, 274, 458
PteI GCGCGC 1 cut(s) 171
RsaI GTAC 3 cut(s) 109, 348, 504
RsaNI GTAC 3 cut(s) 108, 347, 503
SacI GAGCTC 1 cut(s) 484
SacII CCGCGG 1 cut(s) 71
SaqAI TTAA 1 cut(s) 1107
SatI GCNGC 6 cut(s) 71, 74, 144, 613, 1001, 1116
Sau3AI GATC 3 cut(s) 219, 686, 1036
Sau96I GGNCC 3 cut(s) 61, 274, 458
SchI GAGTC 4 cut(s) 235, 276, 329, 866
ScrFI CCNGG 3 cut(s) 272, 662, 684
SduI GDGCHC 2 cut(s) 378, 484
SfaNI GCATC 2 cut(s) 779, 995
Sfr303I CCGCGG 1 cut(s) 71
SgrBI CCGCGG 1 cut(s) 71
SinI GGWCC 1 cut(s) 458
Sse9I AATT 3 cut(s) 754, 921, 1020
SspMI CTAG 2 cut(s) 399, 1112
SstI GAGCTC 1 cut(s) 484
StyD4I CCNGG 3 cut(s) 270, 660, 682
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 3 cut(s) 253, 351, 1225
TaiI ACGT 2 cut(s) 728, 739
TaqI TCGA 6 cut(s) 92, 130, 150, 353, 638, 1197
TasI AATT 3 cut(s) 754, 921, 1020
TauI GCSGC 2 cut(s) 73, 76
TfiI GAWTC 6 cut(s) 4, 42, 56, 303, 313, 1158
Tru1I TTAA 1 cut(s) 1107
Tru9I TTAA 1 cut(s) 1107
TscAI CASTG 1 cut(s) 1228
TseFI GTSAC 3 cut(s) 247, 1081, 1165
TseI GCWGC 4 cut(s) 143, 612, 1000, 1115
Tsp45I GTSAC 3 cut(s) 247, 1081, 1165
TspDTI ATGAA 7 cut(s) 444, 768, 983, 1004, 1080, 1116, 1137
TspGWI ACGGA 1 cut(s) 1147
TspRI CASTG 1 cut(s) 1228
Van91I CCANNNNNTGG 1 cut(s) 498
VpaK11BI GGWCC 1 cut(s) 458
XagI CCTNNNNNAGG 1 cut(s) 687
XapI RAATTY 2 cut(s) 754, 921
XcmI CCANNNNNNNNNTGG 1 cut(s) 964
XmnI GAANNNNTTC 2 cut(s) 302, 924
XspI CTAG 2 cut(s) 399, 1112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.