pycom10g28640

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001648
Physical Location & Seq
Reverse (-)
29160677 .. 29162340
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g28640.1

Sequence Viewer

Length: 1122 bp
ATGTTCAGGTCGATCAATGCTGAGTGTGTTGGTGGTCCTGCAACAGTTCTGACTCCTAAGCACTACGAAGAATGCTTGGCTGTTGTAGCTCATAAATCTTCTTCTGGAATCCTTATGTGCTGTCGGCAACTTGGGGACTACAAGGTTAAGCCCTTCAGTCTTGCTAAATTGCGCGGGTATTCTAATCAAGCAATTCTGTCTTCTCTACATGTCCACATCTCCAAGCAAAAAACCAAAGCCAAGTACCATAAATTATGTGATGAAGATGAAGTAGAGTTAGCACCTGATGATCCTCTGAGGGTGAGCCTTAAGAGAAACAGAACCAGCAAGGGGAATCAGGGACATTCTGTTGTTAAAAATTTGGTCACTGGGAATCATTTACCACATTGCGTTCCAACATATCCAAAGTTGAAATTAAAATTATCAAGGAAATCCATGGGAGTTAAGATTGCTGGATCGGAGCCCATACGCCCAGTTTCTTTTAAAGTTGATGAAAAGATAGAGTTGCTCTGTCAGGATAGTGGCATACGAGGATGTTGGTTTAGGTGTCAGGTCTTGCAAACATCTCAAAAGATTCTCAAAGTTCAATATGATGATTTGCAAGATGTGGATGGATCTGGCAATCTGGAGGAATGGGTCCCTGCCTTAAAAGTTGCTGCTCCTGATAAACTAGGCATGAGATGCTCAGGCCGCCTGACAATCCGACCCTGTTGTCTCAATGATTCTACAGAGGGTTGTTTTGAGGTTGGAGTAGCAGTTGATGCGTGGTGGTGTGATGGCTGGTGGGAAGGTGTTGTTACTGGAGTTAACATTTCTGGGACTGATAGTATACAAGTCTACTTCCCTGGTGAGAAGAAGCTTATGAGTTTCCAGAGAAAGGATGTGAGGGCTTCCAGAGACTGGGTTGAGAACAGGTGGGTTGATGTGAAGGCAAAGCCTGATGTATTCTCATACGTATCTGAAAATATCAGTTCTAGCATGAAGCTCTTATCAGTCTCTTCTAGCTCTATGGCAAAAGCTTCTTCTAAACTTGAAGCTGTTGAAGAGAAACAGGAATCACCAGATTTAGCCCCACTGCATGAGGAGTTGGGAAAGGTGACGAGAATGAATATGCGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet BAH

Protein Analysis

374

Amino Acids

41.58

Weight (kDa)

8.88

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 165 - 237 4.8e-16 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 711
AccB7I CCANNNNNTGG 1 cut(s) 900
AccI GTMKAC 2 cut(s) 829, 837
AccII CGCG 1 cut(s) 174
AciI CCGC 3 cut(s) 174, 691, 1114
AclWI GGATC 3 cut(s) 284, 463, 622
AcsI RAATTY 1 cut(s) 358
AcuI CTGAAG 1 cut(s) 139
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 3 cut(s) 330, 877, 900
AflII CTTAAG 1 cut(s) 308
AflIII ACRYGT 1 cut(s) 208
AgsI TTSAA 4 cut(s) 412, 587, 1034, 1043
AjnI CCWGG 1 cut(s) 844
AluBI AGCT 6 cut(s) 89, 859, 985, 1005, 1019, 1037
AluI AGCT 6 cut(s) 89, 859, 985, 1005, 1019, 1037
Alw26I GTCTC 3 cut(s) 719, 891, 1000
AlwI GGATC 3 cut(s) 284, 463, 622
AlwNI CAGNNNCTG 1 cut(s) 900
AoxI GGCC 1 cut(s) 688
ApeKI GCWGC 1 cut(s) 656
ApoI RAATTY 1 cut(s) 358
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 2 cut(s) 35, 637
AsuHPI GGTGA 4 cut(s) 313, 860, 1050, 1108
AvaII GGWCC 2 cut(s) 35, 637
BanII GRGCYC 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 192
BbvI GCAGC 1 cut(s) 643
BccI CCATC 2 cut(s) 605, 770
BciT130I CCWGG 1 cut(s) 846
BcoDI GTCTC 3 cut(s) 719, 891, 1000
BfaI CTAG 3 cut(s) 671, 975, 1002
BfmI CTRYAG 1 cut(s) 726
BfrI CTTAAG 1 cut(s) 308
BisI GCNGC 2 cut(s) 657, 691
BlsI GCNGC 2 cut(s) 658, 692
Bme1390I CCNGG 1 cut(s) 846
Bme18I GGWCC 2 cut(s) 35, 637
BmgT120I GGNCC 2 cut(s) 35, 637
BmiI GGNNCC 3 cut(s) 462, 638, 639
BmrFI CCNGG 1 cut(s) 846
BmrI ACTGGG 3 cut(s) 378, 467, 910
BmsI GCATC 2 cut(s) 671, 751
BmuI ACTGGG 3 cut(s) 378, 467, 910
BpiI GAAGAC 1 cut(s) 192
BpmI CTGGAG 2 cut(s) 647, 822
Bpu10I CCTNAGC 2 cut(s) 57, 685
BsaAI YACGTR 1 cut(s) 955
BsaJI CCNNGG 2 cut(s) 435, 844
BsaXI ACNNNNNCTCC 2 cut(s) 741, 771
Bsc4I CCNNNNNNNGG 3 cut(s) 330, 877, 900
Bse1I ACTGG 4 cut(s) 373, 473, 805, 905
Bse3DI GCAATG 1 cut(s) 385
BseBI CCWGG 1 cut(s) 846
BseDI CCNNGG 2 cut(s) 435, 844
BseGI GGATG 3 cut(s) 539, 616, 886
BseLI CCNNNNNNNGG 3 cut(s) 330, 877, 900
BseMI GCAATG 1 cut(s) 385
BseMII CTCAG 3 cut(s) 12, 287, 699
BseNI ACTGG 4 cut(s) 373, 473, 805, 905
BseRI GAGGAG 1 cut(s) 1097
BseXI GCAGC 1 cut(s) 643
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 690
BslFI GGGAC 4 cut(s) 149, 354, 623, 832
BslI CCNNNNNNNGG 3 cut(s) 330, 877, 900
BsmAI GTCTC 3 cut(s) 719, 891, 1000
BsmFI GGGAC 4 cut(s) 149, 354, 623, 832
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 1 cut(s) 690
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 4 cut(s) 12, 289, 455, 614
Bsp19I CCATGG 1 cut(s) 435
BspACI CCGC 3 cut(s) 174, 691, 1114
BspANI GGCC 1 cut(s) 690
BspCNI CTCAG 3 cut(s) 13, 288, 698
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 3 cut(s) 462, 638, 639
BspPI GGATC 3 cut(s) 284, 463, 622
BspTI CTTAAG 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 385
BsrI ACTGG 4 cut(s) 373, 473, 805, 905
BssECI CCNNGG 2 cut(s) 435, 844
BssMI GATC 4 cut(s) 12, 289, 455, 614
BssNAI GTATAC 1 cut(s) 830
BssT1I CCWWGG 1 cut(s) 435
Bst1107I GTATAC 1 cut(s) 830
Bst2UI CCWGG 1 cut(s) 846
Bst4CI ACNGT 1 cut(s) 46
Bst6I CTCTTC 2 cut(s) 1003, 1038
BstAFI CTTAAG 1 cut(s) 308
BstAPI GCANNNNNTGC 2 cut(s) 681, 761
BstBAI YACGTR 1 cut(s) 955
BstDEI CTNAG 4 cut(s) 21, 57, 296, 685
BstDSI CCRYGG 1 cut(s) 435
BstF5I GGATG 3 cut(s) 539, 616, 886
BstFNI CGCG 1 cut(s) 174
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 4 cut(s) 15, 292, 458, 617
BstMAI GTCTC 3 cut(s) 719, 891, 1000
BstMBI GATC 4 cut(s) 12, 289, 455, 614
BstMWI GCNNNNNNNGC 4 cut(s) 86, 681, 690, 761
BstNI CCWGG 1 cut(s) 846
BstNSI RCATGY 1 cut(s) 212
BstSCI CCNGG 1 cut(s) 844
BstSFI CTRYAG 1 cut(s) 726
BstSNI TACGTA 1 cut(s) 955
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 1 cut(s) 643
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 614
BstYI RGATCY 1 cut(s) 614
BstZ17I GTATAC 1 cut(s) 830
BsuRI GGCC 1 cut(s) 690
BtgI CCRYGG 1 cut(s) 435
BtsCI GGATG 3 cut(s) 539, 616, 886
BtsI GCAGTG 1 cut(s) 1073
BtsIMutI CAGTG 2 cut(s) 366, 1073
CaiI CAGNNNCTG 1 cut(s) 900
CfoI GCGC 1 cut(s) 174
Cfr13I GGNCC 2 cut(s) 35, 637
Csp6I GTAC 1 cut(s) 244
CviAII CATG 5 cut(s) 209, 436, 676, 979, 1079
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 4 cut(s) 21, 57, 296, 685
DpnI GATC 4 cut(s) 14, 291, 457, 616
DpnII GATC 4 cut(s) 12, 289, 455, 614
DraI TTTAAA 1 cut(s) 484
DrdI GACNNNNNNGTC 1 cut(s) 711
DseDI GACNNNNNNGTC 1 cut(s) 711
Eam1104I CTCTTC 2 cut(s) 1003, 1038
EarI CTCTTC 2 cut(s) 1003, 1038
Eco105I TACGTA 1 cut(s) 955
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 465
Eco47I GGWCC 2 cut(s) 35, 637
Eco57I CTGAAG 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 637
EcoRII CCWGG 1 cut(s) 844
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 5 cut(s) 212, 439, 679, 982, 1082
FaqI GGGAC 4 cut(s) 149, 354, 623, 832
FatI CATG 5 cut(s) 208, 435, 675, 978, 1078
FauI CCCGC 1 cut(s) 167
FblI GTMKAC 2 cut(s) 829, 837
Fnu4HI GCNGC 2 cut(s) 657, 691
FokI GGATG 3 cut(s) 546, 623, 893
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 2 cut(s) 657, 691
FspBI CTAG 3 cut(s) 671, 975, 1002
GlaI GCGC 1 cut(s) 173
GluI GCNGC 2 cut(s) 657, 691
GsuI CTGGAG 2 cut(s) 647, 822
HaeIII GGCC 1 cut(s) 690
HhaI GCGC 1 cut(s) 174
Hin1II CATG 5 cut(s) 212, 439, 679, 982, 1082
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HincII GTYRAC 1 cut(s) 808
HindII GTYRAC 1 cut(s) 808
HindIII AAGCTT 2 cut(s) 857, 1017
HinfI GANTC 7 cut(s) 52, 108, 334, 373, 574, 722, 1055
HpaI GTTAAC 1 cut(s) 808
HphI GGTGA 4 cut(s) 313, 860, 1050, 1108
Hpy166II GTNNAC 4 cut(s) 214, 808, 830, 838
Hpy188I TCNGA 5 cut(s) 51, 297, 460, 704, 961
Hpy188III TCNNGA 6 cut(s) 105, 515, 626, 662, 871, 894
Hpy8I GTNNAC 4 cut(s) 214, 808, 830, 838
HpyAV CCTTC 3 cut(s) 163, 782, 922
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 1 cut(s) 954
HpyCH4V TGCA 4 cut(s) 41, 559, 601, 1078
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 681, 690, 761
HpyF3I CTNAG 4 cut(s) 21, 57, 296, 685
HpySE526I ACGT 1 cut(s) 954
Hsp92II CATG 5 cut(s) 212, 439, 679, 982, 1082
HspAI GCGC 1 cut(s) 172
KflI GGGWCCC 1 cut(s) 637
KspAI GTTAAC 1 cut(s) 808
Kzo9I GATC 4 cut(s) 12, 289, 455, 614
LmnI GCTCC 2 cut(s) 460, 664
Lsp1109I GCAGC 1 cut(s) 643
LweI GCATC 2 cut(s) 671, 751
MaeI CTAG 3 cut(s) 671, 975, 1002
MaeII ACGT 1 cut(s) 954
MaeIII GTNAC 3 cut(s) 364, 796, 1096
MalI GATC 4 cut(s) 14, 291, 457, 616
MboI GATC 4 cut(s) 12, 289, 455, 614
MboII GAAGA 9 cut(s) 80, 90, 93, 192, 275, 865, 990, 1014, 1055
MflI RGATCY 1 cut(s) 614
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 6 cut(s) 167, 192, 251, 358, 413, 419
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 3 cut(s) 419, 727, 727
MnlI CCTC 8 cut(s) 291, 303, 524, 622, 724, 736, 879, 1075
MseI TTAA 8 cut(s) 147, 309, 354, 416, 444, 483, 647, 807
MspCI CTTAAG 1 cut(s) 308
MspR9I CCNGG 1 cut(s) 846
Mva1269I GAATGC 1 cut(s) 77
MvaI CCWGG 1 cut(s) 846
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 4 cut(s) 86, 681, 690, 761
NcoI CCATGG 1 cut(s) 435
NdeII GATC 4 cut(s) 12, 289, 455, 614
NlaIII CATG 5 cut(s) 212, 439, 679, 982, 1082
NlaIV GGNNCC 3 cut(s) 462, 638, 639
NmuCI GTSAC 2 cut(s) 364, 1096
NspI RCATGY 1 cut(s) 212
PciI ACATGT 1 cut(s) 208
PctI GAATGC 1 cut(s) 77
PfeI GAWTC 6 cut(s) 108, 334, 373, 574, 722, 1055
PflMI CCANNNNNTGG 1 cut(s) 900
PkrI GCNGC 2 cut(s) 658, 692
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 955
PpuMI RGGWCCY 1 cut(s) 637
PscI ACATGT 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 637
Psp6I CCWGG 1 cut(s) 844
PspGI CCWGG 1 cut(s) 844
PspN4I GGNNCC 3 cut(s) 462, 638, 639
PspPI GGNCC 2 cut(s) 35, 637
PspPPI RGGWCCY 1 cut(s) 637
PstNI CAGNNNCTG 1 cut(s) 900
PsuI RGATCY 1 cut(s) 614
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
SaqAI TTAA 8 cut(s) 147, 309, 354, 416, 444, 483, 647, 807
SatI GCNGC 2 cut(s) 657, 691
Sau3AI GATC 4 cut(s) 12, 289, 455, 614
Sau96I GGNCC 2 cut(s) 35, 637
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 1 cut(s) 846
SduI GDGCHC 1 cut(s) 465
SfaNI GCATC 2 cut(s) 671, 751
SfcI CTRYAG 1 cut(s) 726
SinI GGWCC 2 cut(s) 35, 637
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SnaBI TACGTA 1 cut(s) 955
Sse9I AATT 6 cut(s) 167, 192, 251, 358, 413, 419
SsiI CCGC 3 cut(s) 174, 691, 1114
SspMI CTAG 3 cut(s) 671, 975, 1002
StyD4I CCNGG 1 cut(s) 844
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 1 cut(s) 957
TaqI TCGA 1 cut(s) 11
TasI AATT 6 cut(s) 167, 192, 251, 358, 413, 419
TauI GCSGC 1 cut(s) 693
TfiI GAWTC 6 cut(s) 108, 334, 373, 574, 722, 1055
Tru1I TTAA 8 cut(s) 147, 309, 354, 416, 444, 483, 647, 807
Tru9I TTAA 8 cut(s) 147, 309, 354, 416, 444, 483, 647, 807
TscAI CASTG 2 cut(s) 373, 1080
TseFI GTSAC 2 cut(s) 364, 1096
TseI GCWGC 1 cut(s) 656
Tsp45I GTSAC 2 cut(s) 364, 1096
TspDTI ATGAA 5 cut(s) 276, 282, 507, 995, 1121
TspRI CASTG 2 cut(s) 373, 1080
Van91I CCANNNNNTGG 1 cut(s) 900
Vha464I CTTAAG 1 cut(s) 308
VpaK11BI GGWCC 2 cut(s) 35, 637
XapI RAATTY 1 cut(s) 358
XceI RCATGY 1 cut(s) 212
XmiI GTMKAC 2 cut(s) 829, 837
XspI CTAG 3 cut(s) 671, 975, 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.