Rh5DG382100

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001648
Physical Location & Seq
Forward (+)
58446950 .. 58455337
8388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG382100.1

Sequence Viewer

Length: 1494 bp
ATGAGATATCAGCATGCCTCCCGAGGAATTCAGATCAAGTCCTTTGTATTCGTCATGGCAAAGGGAGAAAATCGGTATGTTGCTTATATGGAAGATATGTATGAAGACAAAAGGGGTCAAAAAAAGGTCAAAGTGAGGTGGTTTCACCGTAATCAGGAAGTTAAGGGGGTAATTCCTCTGCAAAATCCTCACCCAAAGGAAGTCTTCATCACGCCATATGTGCAGGTCATTAGTGCAGAGTGTGTTGATGGTCCTGCAACAGTTTTAACTCGTGAACATTATGAAAAGTGCGTACTTGTGTTTCCTCAGGCTTTATTGGCCAAAGTACATCTTTGCTACAGGCAGTTTAAAAGTAAAAGGGTGAAGCCATTTGATTTGAGTAAGATGCATGGCTATTTTGTTCAGCCAATTCTCTCTTATTTGAGTCCCAACTTTTTACCAAATTCAGAGTGTATAAGTGGTAGTCTGATTGGAAAAGAAGATAAGTTGGGCCCAAGTGACTATGTAAAGCTGGGAACTAAGAGGACTAGAAATGGAAGGCCATGCCAGAGGGTTCCAACTAATCAAAACTCAAACTTTGGTTTTCTGGACAGGAGGTTGCTTTCTTGGTATACCCCACTGTTTAAGGTTGATGAAAAGATAGAATTGCTATGCCAAGATAGTGGCATTCGGGGCTGCTGGTTTAGGTGTACGGTCTTGCAGACATCTCGAAAGCAGATTAGAGTCCATTACAATGATTTGCTTGATGAGGAAGGATGTGGCAATCTTGAGGAATGGATCCCTGCTTTTAGACTGGCTAAGCCTGATAAGATTGGCATGCGGCATCTAGGCCGTCCAATGATACGGCCAGCCCCTCCTGAGGTGCAAATGGACCTTCCTCTTGAGGTTGGAGCTGCAATTGATGCATGGTGGAGCGATGGCTGGTGGGAAGGGATTATAACTGGATTTGGCAGCTGTGATGATAATTTTCAAGTTTACTTTCCGGGGGAAAGCTTGCTCTTGAACATGGACAAAAAGGATCTAAGAATATCTAGAGATTGGTTGGGGGACCAGTGGGTTGATATTGAGGCGAAGCCTGATGTCCTCTCAGCCATATCTTCAACAATTAGCTCTTGCACTAGGCCCTTTGTGTCATCCAACATTGCCAAGGATGTTGAGGATGTTGATTGTGGTGGTAATACTCTGTCATATTCTGAACTTCCTTCTAGTTCTAGACATGAAAAAGATGAACTAGGCGCATCTCCCAGTTCTGATCATTTTCCTGAAAAAATGGACTTGGTCAATGATAAGAAGTCACCATCAATAAAGGACATTGGTACTGAAGGTGTTGATGGCAATGACAGTTCTGGTGATATACATGTCCAAGGTAATGATGATGATGATGATGACAAATATGACCACAATGGTGTTGATGAGGGGCAAGAAGGCATGGAGGTGTTTGAAACTCCAGGGAAGAGCCCTAGAGCTGTAGAAATCATGTCAGTGACATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet BAH

Protein Analysis

497

Amino Acids

56.32

Weight (kDa)

5.58

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAH PF01426 14 - 98 6.4e-09 BAH domain
Agenet PF05641 212 - 286 1.6e-16 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 938
Acc36I ACCTGC 1 cut(s) 214
AccI GTMKAC 1 cut(s) 611
AciI CCGC 1 cut(s) 820
AclWI GGATC 3 cut(s) 772, 785, 1026
AcoI YGGCCR 2 cut(s) 318, 845
AcsI RAATTY 2 cut(s) 27, 442
AcuI CTGAAG 1 cut(s) 1341
AfaI GTAC 4 cut(s) 294, 327, 691, 1318
AfiI CCNNNNNNNGG 2 cut(s) 154, 859
AflIII ACRYGT 1 cut(s) 1357
AgsI TTSAA 4 cut(s) 971, 1003, 1101, 1442
AjnI CCWGG 1 cut(s) 1447
AleI CACNNNNGTG 1 cut(s) 1404
AluBI AGCT 6 cut(s) 511, 893, 954, 993, 1110, 1466
AluI AGCT 6 cut(s) 511, 893, 954, 993, 1110, 1466
AlwI GGATC 3 cut(s) 772, 785, 1026
Ama87I CYCGRG 1 cut(s) 21
AoxI GGCC 6 cut(s) 318, 490, 539, 829, 845, 1121
ApaI GGGCCC 1 cut(s) 494
ApeKI GCWGC 3 cut(s) 675, 893, 951
ApoI RAATTY 2 cut(s) 27, 442
AspLEI GCGC 1 cut(s) 1238
AspS9I GGNCC 6 cut(s) 251, 490, 491, 871, 1048, 1122
AsuC2I CCSGG 1 cut(s) 984
AsuHPI GGTGA 5 cut(s) 137, 182, 373, 1287, 1361
AvaI CYCGRG 1 cut(s) 21
AvaII GGWCC 3 cut(s) 251, 871, 1048
AxyI CCTNAGG 2 cut(s) 306, 858
BaeGI GKGCMC 1 cut(s) 494
BalI TGGCCA 1 cut(s) 320
BamHI GGATCC 1 cut(s) 777
BanII GRGCYC 2 cut(s) 494, 1460
BauI CACGAG 1 cut(s) 270
BbsI GAAGAC 2 cut(s) 111, 196
BbvI GCAGC 3 cut(s) 662, 880, 963
BccI CCATC 4 cut(s) 242, 911, 1306, 1325
BceAI ACGGC 2 cut(s) 816, 860
BcgI CGANNNNNNTGC 2 cut(s) 689, 723
BciT130I CCWGG 1 cut(s) 1449
BclI TGATCA 1 cut(s) 1252
BcnI CCSGG 1 cut(s) 984
BfaI CTAG 8 cut(s) 528, 827, 1032, 1119, 1206, 1212, 1232, 1461
BfmI CTRYAG 2 cut(s) 337, 1467
BfuAI ACCTGC 1 cut(s) 214
BisI GCNGC 4 cut(s) 676, 821, 894, 952
BlpI GCTNAGC 1 cut(s) 798
BlsI GCNGC 4 cut(s) 677, 822, 895, 953
Bme1390I CCNGG 2 cut(s) 984, 1449
Bme18I GGWCC 3 cut(s) 251, 871, 1048
BmeT110I CYCGRG 1 cut(s) 21
BmgT120I GGNCC 6 cut(s) 251, 490, 491, 871, 1048, 1122
BmiI GGNNCC 4 cut(s) 492, 555, 779, 1049
BmrFI CCNGG 2 cut(s) 984, 1449
BmrI ACTGGG 1 cut(s) 1239
BmsI GCATC 4 cut(s) 375, 832, 892, 1247
BmuI ACTGGG 1 cut(s) 1239
BpiI GAAGAC 2 cut(s) 111, 196
BpmI CTGGAG 1 cut(s) 1431
Bpu1102I GCTNAGC 1 cut(s) 798
BpuEI CTTGAG 2 cut(s) 788, 902
BpuMI CCSGG 1 cut(s) 984
BsaJI CCNNGG 5 cut(s) 22, 983, 1146, 1363, 1448
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 859
Bse1I ACTGG 4 cut(s) 798, 946, 1051, 1245
Bse21I CCTNAGG 2 cut(s) 306, 858
Bse3DI GCAATG 2 cut(s) 1140, 1342
BseBI CCWGG 1 cut(s) 1449
BseDI CCNNGG 5 cut(s) 22, 983, 1146, 1363, 1448
BseGI GGATG 4 cut(s) 761, 1133, 1156, 1165
BseLI CCNNNNNNNGG 2 cut(s) 154, 859
BseMI GCAATG 2 cut(s) 1140, 1342
BseMII CTCAG 3 cut(s) 320, 849, 1101
BseNI ACTGG 4 cut(s) 798, 946, 1051, 1245
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 3 cut(s) 662, 880, 963
BseYI CCCAGC 1 cut(s) 511
BsgI GTGCAG 2 cut(s) 242, 255
BshFI GGCC 6 cut(s) 320, 492, 541, 831, 847, 1123
BsiHKCI CYCGRG 1 cut(s) 21
BsiSI CCGG 1 cut(s) 983
BslFI GGGAC 2 cut(s) 411, 1061
BslI CCNNNNNNNGG 2 cut(s) 154, 859
BsmFI GGGAC 2 cut(s) 411, 1061
BsmI GAATGC 1 cut(s) 666
BsnI GGCC 6 cut(s) 320, 492, 541, 831, 847, 1123
BsoBI CYCGRG 1 cut(s) 21
Bsp120I GGGCCC 1 cut(s) 490
Bsp1286I GDGCHC 2 cut(s) 494, 1460
Bsp143I GATC 4 cut(s) 33, 777, 1018, 1252
Bsp1720I GCTNAGC 1 cut(s) 798
BspACI CCGC 1 cut(s) 820
BspANI GGCC 6 cut(s) 320, 492, 541, 831, 847, 1123
BspCNI CTCAG 3 cut(s) 319, 850, 1100
BspLI GGNNCC 4 cut(s) 492, 555, 779, 1049
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 3 cut(s) 772, 785, 1026
BspQI GCTCTTC 1 cut(s) 1448
BsrDI GCAATG 2 cut(s) 1140, 1342
BsrI ACTGG 4 cut(s) 798, 946, 1051, 1245
BssECI CCNNGG 5 cut(s) 22, 983, 1146, 1363, 1448
BssMI GATC 4 cut(s) 33, 777, 1018, 1252
BssNAI GTATAC 1 cut(s) 612
BssSI CACGAG 1 cut(s) 270
BssT1I CCWWGG 2 cut(s) 1146, 1363
Bst1107I GTATAC 1 cut(s) 612
Bst2BI CACGAG 1 cut(s) 270
Bst2UI CCWGG 1 cut(s) 1449
Bst4CI ACNGT 5 cut(s) 149, 262, 621, 694, 1343
Bst6I CTCTTC 1 cut(s) 1448
BstAPI GCANNNNNTGC 1 cut(s) 902
BstC8I GCNNGC 4 cut(s) 15, 818, 849, 995
BstDEI CTNAG 6 cut(s) 306, 519, 798, 858, 1022, 1087
BstF5I GGATG 4 cut(s) 761, 1133, 1156, 1165
BstHHI GCGC 1 cut(s) 1238
BstKTI GATC 4 cut(s) 36, 780, 1021, 1255
BstMBI GATC 4 cut(s) 33, 777, 1018, 1252
BstMWI GCNNNNNNNGC 4 cut(s) 220, 317, 672, 902
BstNI CCWGG 1 cut(s) 1449
BstNSI RCATGY 3 cut(s) 17, 820, 1361
BstSCI CCNGG 2 cut(s) 982, 1447
BstSFI CTRYAG 2 cut(s) 337, 1467
BstSLI GKGCMC 1 cut(s) 494
BstV1I GCAGC 3 cut(s) 662, 880, 963
BstV2I GAAGAC 2 cut(s) 111, 196
BstX2I RGATCY 2 cut(s) 777, 1018
BstXI CCANNNNNNTGG 1 cut(s) 662
BstYI RGATCY 2 cut(s) 777, 1018
BstZ17I GTATAC 1 cut(s) 612
Bsu36I CCTNAGG 2 cut(s) 306, 858
BsuRI GGCC 6 cut(s) 320, 492, 541, 831, 847, 1123
BtgZI GCGATG 1 cut(s) 930
BtsCI GGATG 4 cut(s) 761, 1133, 1156, 1165
BtsIMutI CAGTG 3 cut(s) 617, 1058, 1488
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 4 cut(s) 15, 818, 849, 995
CfoI GCGC 1 cut(s) 1238
Cfr13I GGNCC 6 cut(s) 251, 490, 491, 871, 1048, 1122
Csp6I GTAC 4 cut(s) 293, 326, 690, 1317
CviQI GTAC 4 cut(s) 293, 326, 690, 1317
DdeI CTNAG 6 cut(s) 306, 519, 798, 858, 1022, 1087
DpnI GATC 4 cut(s) 35, 779, 1020, 1254
DpnII GATC 4 cut(s) 33, 777, 1018, 1252
DraI TTTAAA 1 cut(s) 349
EaeI YGGCCR 2 cut(s) 318, 845
Eam1104I CTCTTC 1 cut(s) 1448
EarI CTCTTC 1 cut(s) 1448
Eco130I CCWWGG 2 cut(s) 1146, 1363
Eco24I GRGCYC 2 cut(s) 494, 1460
Eco32I GATATC 1 cut(s) 8
Eco47I GGWCC 3 cut(s) 251, 871, 1048
Eco57I CTGAAG 1 cut(s) 1341
Eco81I CCTNAGG 2 cut(s) 306, 858
Eco88I CYCGRG 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 1122
EcoRI GAATTC 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 1447
EcoRV GATATC 1 cut(s) 8
EcoT14I CCWWGG 2 cut(s) 1146, 1363
EcoT22I ATGCAT 2 cut(s) 390, 907
EcoT38I GRGCYC 2 cut(s) 494, 1460
ErhI CCWWGG 2 cut(s) 1146, 1363
FalI AAGNNNNNCTT 4 cut(s) 188, 220, 315, 347
FaqI GGGAC 2 cut(s) 411, 1061
FauNDI CATATG 1 cut(s) 217
FbaI TGATCA 1 cut(s) 1252
FblI GTMKAC 1 cut(s) 611
Fnu4HI GCNGC 4 cut(s) 676, 821, 894, 952
FokI GGATG 4 cut(s) 768, 1120, 1163, 1172
FriOI GRGCYC 2 cut(s) 494, 1460
Fsp4HI GCNGC 4 cut(s) 676, 821, 894, 952
FspBI CTAG 8 cut(s) 528, 827, 1032, 1119, 1206, 1212, 1232, 1461
GlaI GCGC 1 cut(s) 1237
GluI GCNGC 4 cut(s) 676, 821, 894, 952
GsaI CCCAGC 1 cut(s) 515
GsuI CTGGAG 1 cut(s) 1431
HaeIII GGCC 6 cut(s) 320, 492, 541, 831, 847, 1123
HapII CCGG 1 cut(s) 983
HhaI GCGC 1 cut(s) 1238
Hin6I GCGC 1 cut(s) 1236
HinP1I GCGC 1 cut(s) 1236
HindIII AAGCTT 1 cut(s) 991
HinfI GANTC 2 cut(s) 424, 723
HpaII CCGG 1 cut(s) 983
HphI GGTGA 5 cut(s) 137, 182, 373, 1287, 1361
Hpy166II GTNNAC 4 cut(s) 275, 612, 690, 976
Hpy188I TCNGA 5 cut(s) 33, 448, 468, 1195, 1252
Hpy8I GTNNAC 4 cut(s) 275, 612, 690, 976
HpyAV CCTTC 7 cut(s) 531, 746, 884, 923, 1212, 1316, 1418
HpyCH4III ACNGT 5 cut(s) 149, 262, 621, 694, 1343
HpyF10VI GCNNNNNNNGC 4 cut(s) 220, 317, 672, 902
HpyF3I CTNAG 6 cut(s) 306, 519, 798, 858, 1022, 1087
HspAI GCGC 1 cut(s) 1236
Ksp22I TGATCA 1 cut(s) 1252
Kzo9I GATC 4 cut(s) 33, 777, 1018, 1252
LguI GCTCTTC 1 cut(s) 1448
LmnI GCTCC 2 cut(s) 890, 912
Lsp1109I GCAGC 3 cut(s) 662, 880, 963
LweI GCATC 4 cut(s) 375, 832, 892, 1247
MaeI CTAG 8 cut(s) 528, 827, 1032, 1119, 1206, 1212, 1232, 1461
MaeIII GTNAC 3 cut(s) 497, 1293, 1483
MalI GATC 4 cut(s) 35, 779, 1020, 1254
MboI GATC 4 cut(s) 33, 777, 1018, 1252
MboII GAAGA 6 cut(s) 104, 116, 196, 491, 1089, 1465
MfeI CAATTG 1 cut(s) 897
MflI RGATCY 2 cut(s) 777, 1018
MhlI GDGCHC 2 cut(s) 494, 1460
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 8 cut(s) 27, 171, 408, 442, 644, 897, 964, 1104
MluNI TGGCCA 1 cut(s) 320
MlyI GAGTC 2 cut(s) 433, 732
MmeI TCCRAC 3 cut(s) 581, 868, 1161
Mox20I TGGCCA 1 cut(s) 320
Mph1103I ATGCAT 2 cut(s) 390, 907
MscI TGGCCA 1 cut(s) 320
MseI TTAA 4 cut(s) 162, 266, 348, 624
MslI CAYNNNNRTG 4 cut(s) 732, 1404, 1433, 1481
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 954
MspI CCGG 1 cut(s) 983
MspR9I CCNGG 2 cut(s) 984, 1449
MunI CAATTG 1 cut(s) 897
Mva1269I GAATGC 1 cut(s) 666
MvaI CCWGG 1 cut(s) 1449
MwoI GCNNNNNNNGC 4 cut(s) 220, 317, 672, 902
NciI CCSGG 1 cut(s) 984
NdeI CATATG 1 cut(s) 217
NdeII GATC 4 cut(s) 33, 777, 1018, 1252
NlaIV GGNNCC 4 cut(s) 492, 555, 779, 1049
NmuCI GTSAC 3 cut(s) 497, 1293, 1483
NsiI ATGCAT 2 cut(s) 390, 907
NspI RCATGY 3 cut(s) 17, 820, 1361
OliI CACNNNNGTG 1 cut(s) 1404
PaeI GCATGC 2 cut(s) 17, 820
PciI ACATGT 1 cut(s) 1357
PciSI GCTCTTC 1 cut(s) 1448
PctI GAATGC 1 cut(s) 666
PflFI GACNNNGTC 1 cut(s) 1277
PkrI GCNGC 4 cut(s) 677, 822, 895, 953
PleI GAGTC 2 cut(s) 432, 731
PpsI GAGTC 2 cut(s) 432, 731
PscI ACATGT 1 cut(s) 1357
PsiI TTATAA 1 cut(s) 938
Psp6I CCWGG 1 cut(s) 1447
PspFI CCCAGC 1 cut(s) 511
PspGI CCWGG 1 cut(s) 1447
PspN4I GGNNCC 4 cut(s) 492, 555, 779, 1049
PspOMI GGGCCC 1 cut(s) 490
PspPI GGNCC 6 cut(s) 251, 490, 491, 871, 1048, 1122
PsuI RGATCY 2 cut(s) 777, 1018
PsyI GACNNNGTC 1 cut(s) 1277
PvuII CAGCTG 1 cut(s) 954
RsaI GTAC 4 cut(s) 294, 327, 691, 1318
RsaNI GTAC 4 cut(s) 293, 326, 690, 1317
RseI CAYNNNNRTG 4 cut(s) 732, 1404, 1433, 1481
SapI GCTCTTC 1 cut(s) 1448
SaqAI TTAA 4 cut(s) 162, 266, 348, 624
SatI GCNGC 4 cut(s) 676, 821, 894, 952
Sau3AI GATC 4 cut(s) 33, 777, 1018, 1252
Sau96I GGNCC 6 cut(s) 251, 490, 491, 871, 1048, 1122
SchI GAGTC 2 cut(s) 433, 732
ScrFI CCNGG 2 cut(s) 984, 1449
SduI GDGCHC 2 cut(s) 494, 1460
SfaNI GCATC 4 cut(s) 375, 832, 892, 1247
SfcI CTRYAG 2 cut(s) 337, 1467
SinI GGWCC 3 cut(s) 251, 871, 1048
SmiMI CAYNNNNRTG 4 cut(s) 732, 1404, 1433, 1481
SmlI CTYRAG 2 cut(s) 767, 881
SmoI CTYRAG 2 cut(s) 767, 881
SphI GCATGC 2 cut(s) 17, 820
Sse9I AATT 8 cut(s) 27, 171, 408, 442, 644, 897, 964, 1104
SsiI CCGC 1 cut(s) 820
SspMI CTAG 8 cut(s) 528, 827, 1032, 1119, 1206, 1212, 1232, 1461
StyD4I CCNGG 2 cut(s) 982, 1447
StyI CCWWGG 2 cut(s) 1146, 1363
TaaI ACNGT 5 cut(s) 149, 262, 621, 694, 1343
TaqI TCGA 1 cut(s) 709
TasI AATT 8 cut(s) 27, 171, 408, 442, 644, 897, 964, 1104
TatI WGTACW 1 cut(s) 325
TauI GCSGC 1 cut(s) 823
Tru1I TTAA 4 cut(s) 162, 266, 348, 624
Tru9I TTAA 4 cut(s) 162, 266, 348, 624
TscAI CASTG 3 cut(s) 624, 1058, 1488
TseFI GTSAC 3 cut(s) 497, 1293, 1483
TseI GCWGC 3 cut(s) 675, 893, 951
Tsp45I GTSAC 3 cut(s) 497, 1293, 1483
TspDTI ATGAA 6 cut(s) 117, 196, 297, 648, 1233, 1242
TspRI CASTG 3 cut(s) 624, 1058, 1488
Tth111I GACNNNGTC 1 cut(s) 1277
VpaK11BI GGWCC 3 cut(s) 251, 871, 1048
XapI RAATTY 2 cut(s) 27, 442
XbaI TCTAGA 2 cut(s) 1031, 1211
XceI RCATGY 3 cut(s) 17, 820, 1361
XmiI GTMKAC 1 cut(s) 611
XspI CTAG 8 cut(s) 528, 827, 1032, 1119, 1206, 1212, 1232, 1461
Zsp2I ATGCAT 2 cut(s) 390, 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.