pycom11g05370

Mitochondrial import inner membrane translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005664
Physical Location & Seq
Forward (+)
4124689 .. 4126701
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 501 bp
ATGGCATCGAATCCGGAGAACGAATCTACGGCCTCGAGCGAGGTCGAGAACCCTCCGATTGAGCCCTTTCGGCTGCCGACGGTGAAGGAGATGCGCGGTCAAGACATTTGGAACAACTGCGCCGTCCGCAGCGTCGTTAGTGGAGTTATGGGTGGTGGGCTTGGGTTGATGATGGGTTTGCTTCTTGGAGCGTTAGACACCCCTATAATGCAAGACGAAATGACTGGTAAACAACAAATTCATTCACCAAGCAAAGTCGATGGGCCGGAGGAGTTGGAGTTCTGCAAAAGCATTTGCAGTTATGGGATTGGTTTTCTCAGCTGCCGAGTGTGTTGTGAAAAGGCACGAGTTAAACATGACACAACAAATACAGTTGTTGCTGGATATGTAACTGGAGGTGCACTGTCAGCCAAGGCTGGTCCGCAATCAGCCTGTGTTGGTTGTGCCGGCTTTGCTGCATTTTCGGTGCTGATAGAGAAGTTTTTGGATAGACATGATTGA

Protein Analysis

167

Amino Acids

17.6

Weight (kDa)

5.2

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 40 - 155 1.4e-17 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 96
AccIII TCCGGA 1 cut(s) 13
AciI CCGC 3 cut(s) 96, 127, 422
AcsI RAATTY 1 cut(s) 237
AloI GAACNNNNNNTCC 2 cut(s) 263, 295
AluBI AGCT 1 cut(s) 321
AluI AGCT 1 cut(s) 321
Alw21I GWGCWC 1 cut(s) 403
Alw44I GTGCAC 1 cut(s) 399
Ama87I CYCGRG 1 cut(s) 34
Aor13HI TCCGGA 1 cut(s) 13
AoxI GGCC 2 cut(s) 30, 263
ApaLI GTGCAC 1 cut(s) 399
ApeKI GCWGC 4 cut(s) 73, 129, 321, 455
ApoI RAATTY 1 cut(s) 237
AspLEI GCGC 2 cut(s) 96, 122
AspS9I GGNCC 2 cut(s) 263, 419
AsuHPI GGTGA 2 cut(s) 94, 237
AvaI CYCGRG 1 cut(s) 34
AvaII GGWCC 1 cut(s) 419
BaeGI GKGCMC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 66
BauI CACGAG 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 403
BbvI GCAGC 4 cut(s) 60, 141, 308, 442
BccI CCATC 2 cut(s) 166, 254
BceAI ACGGC 2 cut(s) 45, 107
BglI GCCNNNNNGGC 1 cut(s) 70
BisI GCNGC 4 cut(s) 74, 130, 322, 456
BlsI GCNGC 4 cut(s) 75, 131, 323, 457
Bme18I GGWCC 1 cut(s) 419
BmeT110I CYCGRG 1 cut(s) 34
BmgT120I GGNCC 2 cut(s) 263, 419
BmsI GCATC 2 cut(s) 14, 81
BpmI CTGGAG 1 cut(s) 414
BsaJI CCNNGG 1 cut(s) 411
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bse118I RCCGGY 1 cut(s) 446
Bse1I ACTGG 2 cut(s) 229, 397
BseAI TCCGGA 1 cut(s) 13
BseDI CCNNGG 1 cut(s) 411
BseMII CTCAG 1 cut(s) 331
BseNI ACTGG 2 cut(s) 229, 397
BseRI GAGGAG 1 cut(s) 284
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 4 cut(s) 60, 141, 308, 442
Bsh1236I CGCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 32, 265
BsiHKAI GWGCWC 1 cut(s) 403
BsiHKCI CYCGRG 1 cut(s) 34
BsiSI CCGG 3 cut(s) 14, 266, 447
BsnI GGCC 2 cut(s) 32, 265
BsoBI CYCGRG 1 cut(s) 34
Bsp1286I GDGCHC 2 cut(s) 66, 403
Bsp13I TCCGGA 1 cut(s) 13
BspACI CCGC 3 cut(s) 96, 127, 422
BspANI GGCC 2 cut(s) 32, 265
BspCNI CTCAG 1 cut(s) 330
BspEI TCCGGA 1 cut(s) 13
BspFNI CGCG 1 cut(s) 96
BsrFI RCCGGY 1 cut(s) 446
BsrI ACTGG 2 cut(s) 229, 397
BssAI RCCGGY 1 cut(s) 446
BssECI CCNNGG 1 cut(s) 411
BssSI CACGAG 1 cut(s) 345
BssT1I CCWWGG 1 cut(s) 411
Bst2BI CACGAG 1 cut(s) 345
Bst4CI ACNGT 3 cut(s) 82, 373, 405
BstC8I GCNNGC 1 cut(s) 448
BstDEI CTNAG 1 cut(s) 317
BstFNI CGCG 1 cut(s) 96
BstHHI GCGC 2 cut(s) 96, 122
BstMWI GCNNNNNNNGC 4 cut(s) 70, 126, 407, 452
BstSLI GKGCMC 1 cut(s) 403
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 4 cut(s) 60, 141, 308, 442
BsuRI GGCC 2 cut(s) 32, 265
BtsIMutI CAGTG 1 cut(s) 401
Cac8I GCNNGC 1 cut(s) 448
CfoI GCGC 2 cut(s) 96, 122
Cfr10I RCCGGY 1 cut(s) 446
Cfr13I GGNCC 2 cut(s) 263, 419
CseI GACGC 1 cut(s) 121
CviAII CATG 2 cut(s) 356, 494
DdeI CTNAG 1 cut(s) 317
Eco130I CCWWGG 1 cut(s) 411
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 419
Eco88I CYCGRG 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 411
FaeI CATG 2 cut(s) 359, 497
FaiI YATR 6 cut(s) 149, 206, 303, 357, 387, 495
FatI CATG 2 cut(s) 355, 493
Fnu4HI GCNGC 4 cut(s) 74, 130, 322, 456
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 4 cut(s) 74, 130, 322, 456
GlaI GCGC 2 cut(s) 95, 121
GluI GCNGC 4 cut(s) 74, 130, 322, 456
GsuI CTGGAG 1 cut(s) 414
HaeIII GGCC 2 cut(s) 32, 265
HapII CCGG 3 cut(s) 14, 266, 447
HgaI GACGC 1 cut(s) 121
HhaI GCGC 2 cut(s) 96, 122
Hin1II CATG 2 cut(s) 359, 497
Hin6I GCGC 2 cut(s) 94, 120
HinP1I GCGC 2 cut(s) 94, 120
HinfI GANTC 2 cut(s) 10, 23
HpaII CCGG 3 cut(s) 14, 266, 447
HphI GGTGA 2 cut(s) 94, 237
Hpy166II GTNNAC 2 cut(s) 230, 401
Hpy188I TCNGA 1 cut(s) 57
Hpy188III TCNNGA 3 cut(s) 14, 46, 101
Hpy8I GTNNAC 2 cut(s) 230, 401
Hpy99I CGWCG 2 cut(s) 82, 137
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 3 cut(s) 82, 373, 405
HpyCH4V TGCA 5 cut(s) 211, 285, 297, 401, 458
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 126, 407, 452
HpyF3I CTNAG 1 cut(s) 317
Hsp92II CATG 2 cut(s) 359, 497
HspAI GCGC 2 cut(s) 94, 120
Kpn2I TCCGGA 1 cut(s) 13
KroI GCCGGC 1 cut(s) 446
KroNI GCCGGC 1 cut(s) 448
LmnI GCTCC 1 cut(s) 188
LpnPI CCDG 8 cut(s) 27, 210, 279, 366, 378, 402, 445, 460
Lsp1109I GCAGC 4 cut(s) 60, 141, 308, 442
LweI GCATC 2 cut(s) 14, 81
MaeIII GTNAC 1 cut(s) 388
MhlI GDGCHC 2 cut(s) 66, 403
MluCI AATT 1 cut(s) 237
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 5 cut(s) 34, 43, 63, 262, 389
MroI TCCGGA 1 cut(s) 13
MroNI GCCGGC 1 cut(s) 446
MseI TTAA 1 cut(s) 351
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 3 cut(s) 14, 266, 447
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 4 cut(s) 70, 126, 407, 452
NaeI GCCGGC 1 cut(s) 448
NgoMIV GCCGGC 1 cut(s) 446
NlaIII CATG 2 cut(s) 359, 497
NmeAIII GCCGAG 1 cut(s) 350
PaeR7I CTCGAG 1 cut(s) 34
PdiI GCCGGC 1 cut(s) 448
PfeI GAWTC 2 cut(s) 10, 23
PkrI GCNGC 4 cut(s) 75, 131, 323, 457
PspPI GGNCC 2 cut(s) 263, 419
PspXI VCTCGAGB 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 321
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 4 cut(s) 74, 130, 322, 456
Sau96I GGNCC 2 cut(s) 263, 419
SduI GDGCHC 2 cut(s) 66, 403
SetI ASST 3 cut(s) 45, 323, 400
SfaNI GCATC 2 cut(s) 14, 81
Sfr274I CTCGAG 1 cut(s) 34
SinI GGWCC 1 cut(s) 419
SlaI CTCGAG 1 cut(s) 34
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 1 cut(s) 237
SsiI CCGC 3 cut(s) 96, 127, 422
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 3 cut(s) 82, 373, 405
TaqI TCGA 4 cut(s) 8, 35, 45, 258
TasI AATT 1 cut(s) 237
TfiI GAWTC 2 cut(s) 10, 23
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 408
TseI GCWGC 4 cut(s) 73, 129, 321, 455
TspDTI ATGAA 1 cut(s) 230
TspRI CASTG 1 cut(s) 408
VneI GTGCAC 1 cut(s) 399
VpaK11BI GGWCC 1 cut(s) 419
XapI RAATTY 1 cut(s) 237
XhoI CTCGAG 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.