RchiOBHm_Chr5g0071211

Mitochondrial import inner membrane translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005664
Physical Location & Seq
Reverse (-)
77095260 .. 77098042
2783 bp
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UTR
Exon/CDS
Intron
PRQ34632

Sequence Viewer

Length: 501 bp
ATGGCCGAAGAGAAGGCAAATGAATCTACAGCCACCAGAGAAGTAGAGAAGGCTCCGATTGAGCCTCTGCGGATGCCCACGGTGGAGGAGATTCGCGGCCAAGATATTTGGAACAACTGTGTCGTGCGCAGTGTCGCTAGTGGAGTTATGGGTGGTGGACTTGGATTGATGATGGGTTTGTTTCTTGGGGCTTTAGACAACCCAGTAATGCAAGATCAAATGACTGGTAGGCAGCAATTTATATACACAGCAAAGCAGATGGGGTCAAGGAGTTGGGGTTCTGCAAAAGCATTTGCAGTGATGGGATTCGTTTTCTCAGCTGCTGAATGTGTTGTTGAAAAGGCACGAGCTAAGCATGACACAACAAATACCGTTGTCGCGGGATGTGTCACTGGAGGTGCATTGTCAGCAAAAGGTGGTCCAAAAGCAGCCTGTGTTGGTTGTGCTGGGTTTGCCACATTTTCGGTGCTGATAGAGAAATTTTTGGATAGACATGATTGA

Protein Analysis

166

Amino Acids

17.58

Weight (kDa)

6.73

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 40 - 155 1.1e-28 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 128
AccII CGCG 2 cut(s) 96, 380
AciI CCGC 3 cut(s) 70, 96, 380
AcoI YGGCCR 2 cut(s) 3, 97
AcsI RAATTY 1 cut(s) 479
AgsI TTSAA 1 cut(s) 338
AloI GAACNNNNNNTCC 2 cut(s) 262, 294
AluBI AGCT 2 cut(s) 320, 350
AluI AGCT 2 cut(s) 320, 350
AlwNI CAGNNNCTG 1 cut(s) 323
AoxI GGCC 2 cut(s) 3, 97
ApeKI GCWGC 3 cut(s) 232, 320, 428
ApoI RAATTY 1 cut(s) 479
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 419
AvaII GGWCC 1 cut(s) 419
BauI CACGAG 1 cut(s) 345
BbvI GCAGC 3 cut(s) 244, 307, 440
BccI CCATC 3 cut(s) 166, 253, 295
BfaI CTAG 1 cut(s) 138
BfmI CTRYAG 1 cut(s) 27
BisI GCNGC 4 cut(s) 97, 233, 321, 429
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 4 cut(s) 98, 234, 322, 430
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 1 cut(s) 419
BmiI GGNNCC 1 cut(s) 54
BmrI ACTGGG 1 cut(s) 197
BmsI GCATC 1 cut(s) 63
BmuI ACTGGG 1 cut(s) 197
BpmI CTGGAG 1 cut(s) 414
Bpu1102I GCTNAGC 1 cut(s) 351
BsaJI CCNNGG 1 cut(s) 78
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bse1I ACTGG 3 cut(s) 203, 229, 397
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 2 cut(s) 78, 389
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 3 cut(s) 203, 229, 397
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 3 cut(s) 244, 307, 440
BseYI CCCAGC 1 cut(s) 446
Bsh1236I CGCG 2 cut(s) 96, 380
BshFI GGCC 2 cut(s) 5, 99
BsnI GGCC 2 cut(s) 5, 99
Bsp143I GATC 1 cut(s) 214
Bsp1720I GCTNAGC 1 cut(s) 351
BspACI CCGC 3 cut(s) 70, 96, 380
BspANI GGCC 2 cut(s) 5, 99
BspCNI CTCAG 1 cut(s) 329
BspFNI CGCG 2 cut(s) 96, 380
BspLI GGNNCC 1 cut(s) 54
BsrI ACTGG 3 cut(s) 203, 229, 397
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 1 cut(s) 214
BssSI CACGAG 1 cut(s) 345
Bst2BI CACGAG 1 cut(s) 345
Bst4CI ACNGT 3 cut(s) 82, 119, 373
Bst6I CTCTTC 1 cut(s) 3
BstDEI CTNAG 2 cut(s) 316, 351
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 2 cut(s) 78, 389
BstFNI CGCG 2 cut(s) 96, 380
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 1 cut(s) 217
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 2 cut(s) 407, 452
BstSFI CTRYAG 1 cut(s) 27
BstUI CGCG 2 cut(s) 96, 380
BstV1I GCAGC 3 cut(s) 244, 307, 440
BsuRI GGCC 2 cut(s) 5, 99
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 2 cut(s) 78, 389
BtsI GCAGTG 2 cut(s) 136, 303
BtsIMutI CAGTG 3 cut(s) 136, 303, 390
CaiI CAGNNNCTG 1 cut(s) 323
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 419
CviAII CATG 2 cut(s) 356, 494
CviJI RGCY 9 cut(s) 5, 32, 53, 64, 99, 191, 320, 350, 431
CviKI_1 RGCY 9 cut(s) 5, 32, 53, 64, 99, 191, 320, 350, 431
DdeI CTNAG 2 cut(s) 316, 351
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
EaeI YGGCCR 2 cut(s) 3, 97
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 419
FaeI CATG 2 cut(s) 359, 497
FaiI YATR 5 cut(s) 149, 242, 244, 357, 495
FatI CATG 2 cut(s) 355, 493
FauI CCCGC 1 cut(s) 373
Fnu4HI GCNGC 4 cut(s) 97, 233, 321, 429
FokI GGATG 2 cut(s) 85, 396
Fsp4HI GCNGC 4 cut(s) 97, 233, 321, 429
FspBI CTAG 1 cut(s) 138
FspI TGCGCA 1 cut(s) 128
GlaI GCGC 1 cut(s) 128
GluI GCNGC 4 cut(s) 97, 233, 321, 429
GsaI CCCAGC 1 cut(s) 450
GsuI CTGGAG 1 cut(s) 414
HaeIII GGCC 2 cut(s) 5, 99
HhaI GCGC 1 cut(s) 129
Hin1II CATG 2 cut(s) 359, 497
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HinfI GANTC 3 cut(s) 23, 91, 306
Hpy166II GTNNAC 1 cut(s) 158
Hpy188I TCNGA 1 cut(s) 57
Hpy8I GTNNAC 1 cut(s) 158
HpyAV CCTTC 2 cut(s) 7, 43
HpyCH4III ACNGT 3 cut(s) 82, 119, 373
HpyCH4V TGCA 4 cut(s) 211, 284, 296, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 407, 452
HpyF3I CTNAG 2 cut(s) 316, 351
Hsp92II CATG 2 cut(s) 359, 497
HspAI GCGC 1 cut(s) 127
Kzo9I GATC 1 cut(s) 214
LmnI GCTCC 1 cut(s) 58
LpnPI CCDG 6 cut(s) 49, 210, 216, 378, 432, 445
Lsp1109I GCAGC 3 cut(s) 244, 307, 440
LweI GCATC 1 cut(s) 63
MaeI CTAG 1 cut(s) 138
MaeIII GTNAC 1 cut(s) 388
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MboII GAAGA 1 cut(s) 20
MluCI AATT 2 cut(s) 236, 479
MnlI CCTC 3 cut(s) 75, 79, 389
MspA1I CMGCKG 1 cut(s) 320
MvnI CGCG 2 cut(s) 96, 380
MwoI GCNNNNNNNGC 2 cut(s) 407, 452
NdeII GATC 1 cut(s) 214
NlaIII CATG 2 cut(s) 359, 497
NlaIV GGNNCC 1 cut(s) 54
NmuCI GTSAC 1 cut(s) 388
NsbI TGCGCA 1 cut(s) 128
PfeI GAWTC 3 cut(s) 23, 91, 306
PkrI GCNGC 4 cut(s) 98, 234, 322, 430
PspFI CCCAGC 1 cut(s) 446
PspN4I GGNNCC 1 cut(s) 54
PspPI GGNCC 1 cut(s) 419
PstNI CAGNNNCTG 1 cut(s) 323
PvuII CAGCTG 1 cut(s) 320
SatI GCNGC 4 cut(s) 97, 233, 321, 429
Sau3AI GATC 1 cut(s) 214
Sau96I GGNCC 1 cut(s) 419
SetI ASST 4 cut(s) 322, 352, 400, 418
SfaNI GCATC 1 cut(s) 63
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 419
Sse9I AATT 2 cut(s) 236, 479
SsiI CCGC 3 cut(s) 70, 96, 380
SspMI CTAG 1 cut(s) 138
TaaI ACNGT 3 cut(s) 82, 119, 373
TasI AATT 2 cut(s) 236, 479
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 3 cut(s) 23, 91, 306
TscAI CASTG 3 cut(s) 136, 303, 397
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 3 cut(s) 232, 320, 428
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 1 cut(s) 36
TspRI CASTG 3 cut(s) 136, 303, 397
VpaK11BI GGWCC 1 cut(s) 419
XapI RAATTY 1 cut(s) 479
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.