Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0002217

Physical Location & Seq

Genomic Coordinates Forward (+)

4249093 .. 4253356

Gene Structure 4264 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3438 bp

ATGCCCAAATTACGCTTTCTCATAGTGGCTTCGGCGGCCCCAATCCTTCTCGGACTGGCTCTCCGAACCTACTCTGGCGCCGACGACGCTGCCAATTCAATTTTCACCACTCGTTTTCTCGTTTGGAGCTTGCTAGTTTCGGTGATGGTCGCCTTTTCTGGCGTTTTCAGCAGATTGCTGCACTGTATGCTTCCTAAAAAGAGACCCGGGGACGGAGTCGTTGTAGAAGTAGAAGTAGGAGGAGACACCGGCAGCTCCGCCCCGTTATCGTCGATCAATAAGCATCGCATCGGCAACTTCGTTGCCGAATCTACTGTCAGCAACAACAGCAGCCTCGCAAGCAGCAACCACGGCAGCGTGGTTGAGAAGGACGTGCCGATCATGGCTTTGGGCCATTCGAACCAGTCCGATATCGACGAGGACCTTCACAGCCGGCAGCTCGCGGTCTATGGCCGCGAGACGATGCGGCGGCTCTTCGCGTCGAATGTTCTCATCTCAGGGATTCAAGGTCTAGGTGCTGAAATTGCAAAGAACCTCATTCTGGCTGGGGTCAAGTCTGTGACTTTGCACGATGAAGGGAATGTGGAGCTATGGGATTTGTCTAGTAACTTTGTTTTTTCCGAGGACGATGTGGGTAAGAACAGAGCACTTGCTTCGGTTCAAAAGTTGCAGGAACTCAACAATGCTGTGGTTGTTAATACCTTGACAACTGAATTGACCAAAGAGCAGCTTTCTAATTTCCAGGTTGTTGTATTCACTGACATCAGTCTCGAAAAAGCCATTGAATTCGATGATTATACACATGATCATCAGCCCCCAATCGCCTTCATTAAAACTGAAGCTAGAGGCCTTTTTGGTTCTGTCTTTTGTGACTTTGGACCTGAGTTCACTGTTTTTGATGTTGATGGGGAGGAACCACACACGGGTATAATTTCATCCATCAGCAATGACAACCCTGCTATAGTGTCATGTGTTGACGACGAGAGACTTGAATTTCAGGATGGGGATCTTGTTGTGTTCTCAGAGGTTAATGGAATGACAGACCTAAATGATGGAAAACCAAGAAGGATAAAAAATGCTAGGGCTTACTCATTCACTCTTGAGGAGGATACCACGAGGTTTGGTGCCTATGAGAAAGGTGGTATTGTCACACAGGTGAAGCAGCCAAAAGTCTTGAAATTTAAGTCATTGAGAGAAGCACTCAATGATCCTGGTGAATTTCTTCCTAGTGATTTCTCCAAGTTTGATCGGCCACCACTCCTACACTTGGCATTTCAAGCACTGGACAAAGTTGTGTCTGAGCTTGGGCGATTCCCTGTTGCTGGTTCAGAAGAGGATGCACAGAAGCTTATATCTATTGCTAGTAACATTAATGAAAAATTGGGAGGTGGTAAGATGGAAGATATTAATCCAAAACTTTTGCGGCACTTTGCCTTTGGTGCAAAGGCGGTACTGAATCCCATGGCTGCCATGTTTGGTGGTATTGTGGGTCAAGAGGTTGTAAAAGCTTGCTCTGGAAAGTTCCATCCACTCTTTCAGTTCTTCTACTTCGACTCAGTAGAATCACTTCCTACGGAGCCACCGGAATCCAGTGATTTGAAACCATTAAATAGCCGTTATGATGCACAAATTTCAGTTTTTGGGTCCAAGCTACAGAAGAAATTGGAGGATGCGAAAATATTTCTAGTTGGATCTGGGGCACTAGGATGTGAGTTCTTAAAAAATTTGGCCCTGATGGGAGTTTCATGTGGCAAGCAAGGGAAGCTAACAGTCACTGATGATGATGTAATTGAGAAGAGTAACCTTAGTAGGCAGTTCCTCTTCCGTGATTGGAACATTGGGCAGCCCAAATCCACGGCTGCTGCCTCCGCTGCTGCATCTATAAATCCTTATCTCAATGTCGAAGCCTTGCAGAATAGAGTTAGCTCTGAAACTGAGGATGTGTTTGATGATGCCTTCTGGGAGAATTTAAGTGTTGTTATCAATGCACTCGATAATGTCAATGCTAGGCTCTATGTTGATCAGAGGTGCTTATATTTCCAGAAGCCACTTCTCGAGTCAGGTACACTGGGCACAAAATGCAACACTCAGATGGTTATTCCTCTCCTAACAGAAAACTATGGTGCCTCAAGAGACCCACCGGAGAAGCAAGCACCCATGTGCACTTTGCACTCATTTCCACACAACATTGACCACTGCTTGACATGGGCTCGATCTGAGTTTGAGGGCTTGCTTGGCAAAACTCCAGCTGAAGTGAATGCATATTTAGCCAAGCCAAGTGAGTATGCTGCTTCGAAGAGGAATGCTGGTGACGCACAGGCCAGGGATACCTTGGAGCGCATTCTTGAGTGCCTTGATAGAGAAAGATGCGAAACATTTCAAGATTGCATTGCATGGGCACGCCTGAAGTTTGAAGATTATTTTTACAACCGCATCAAACAGCTGATCTATACTTTTCCTGAAGATGCTACAACCAGTAGTGGGACTCCATTCTGGTCTGCCCCTAAGCGATTCCCCCATCCTCTACAGTTCTCAACTGCTGATCCTGGTCACCTTAATTTTGTTATGGCAGCATCCATACTAAGGGCAGAAACATTTGGGATCCCAATCCCCCACTGGGTTAGAAACTCTAAGAAGTTTGCTGAAGCTGTTGAGAAAGTGATGGTCCCAGAATTTCAGCCCCGGAGAGATGCAAACATAGTGACCGACGATAATGCTACCAATTTAACTTCTCAATCTACAGATGATGCACAGGTCATTGATGACTTGATCATCAAGTTAGAGCATTGTCGAGAGAAACTCTCACCAGGGTTCAGGATGAAACCAATTCAGTTCGAGAAGGACGATGATACAAATTACCATATGGATCTGATAGCTGGGCTTGCCAATATGAGGGCTAGAAATTACGGCATTCCTGAGGTTGACAAGCTGAAAGCCAAGTTTATTGCCGGCAAGATTATCCCCGCAATTGCTACCACCACGGCAATGGCCACAGGACTGGTGTGCCTGGAGCTTTACAAAGTTTTGGACGGAGGCCACAAACTAGAAGACTATAGAAACACGTTTGCAAATCTAGCTCTACCTTTGTTCGCAATGGCAGAGCCAGTTCCGGTGAAGGTCATCAAGCACCGTAACATGAGCTGGACCATTTGGGATAGGTGGATCGTGAGGGGCAATCCCACTCTGAGAGAATTTATTCAGTGGCTCAAGGACAGAGGACTGGATGCTTATAGCATCTCATATGCCAGTTCCCTGCTTTATAATACCATGTTTGCACGACACCAAGACCGAATGGACAAGAAGATGGTGGATCTGGCTAGGGAAGTGGGGGGTTTGGAACTGTCTCCAAACCGCTCCCACTTCGATGTTGTTGTGGCTTGTGAGGACGATGAGGGGAGCGAAGTTGACATCCCTCTGGTATCCATCTACTTCCGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030054 GO:0032446 GO:0033554 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 ko05012 map04120 map05012

Pfam Domains

Protein Families

E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1146

Amino Acids

127.19

Weight (kDa)

5.36

Isoelectric Point (pI)

35.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	143 - 519	5.9e-25	ThiF family
E1_FCCH	PF16190	317 - 387	6.9e-27	Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB	PF16191	388 - 458	2.9e-24	Ubiquitin-activating enzyme E1 four-helix bundle
ThiF	PF00899	540 - 1031	7.5e-61	ThiF family
UBA_E1_SCCH	PF10585	727 - 980	3.4e-88	Ubiquitin-activating enzyme, SCCH domain
E1_UFD	PF09358	1051 - 1140	1.6e-21	Ubiquitin fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30110 AT5G06460
fragaria_vesca	FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_6g10930 FvH4_6g10930 FvH4_6g10930
malus_domestica	MD04G1163500.v1.1 MD11G1065700.v1.1 MD12G1177300.v1.1
prunus_persica	Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G282900_v2.0.a1
pyrus_communis	pycom04g14620 pycom11g05570 pycom12g16790
rosa_chinensis	RchiOBHm_Chr3g0462201 RchiOBHm_Chr5g0070911
rosa_laevigata	RLG00000024878 RLG00000036170
rosa_multiflora	Rmu_sc0002944.1_g000004 Rmu_sc0005970.1_g000009 Rmu_sc0006715.1_g000003
rosa_roxburghii	Rroxscaffold_1G00010290 Rroxscaffold_6G00417690
rosa_rugosa	Rorug03G0054900 Rorug05G0408500 Rorug05G0408500 Rorug05G0408500
rosa_samantha	Rh3CG119500 Rh5AG464600 Rh5DG494900
rosa_wichuraiana	Rw3G009590 Rw5G043220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3261
AccB1I	GGYRCC	3 cut(s)	77, 1124, 2123
AccBSI	CCGCTC	1 cut(s)	3354
AccII	CGCG	3 cut(s)	443, 456, 479
AclWI	GGATC	9 cut(s)	1014, 1202, 1699, 2537, 2596, 2609, 2874, 3170, 3318
AcoI	YGGCCR	3 cut(s)	451, 1250, 2988
AcsI	RAATTY	9 cut(s)	785, 992, 1178, 1217, 1629, 1723, 1966, 2672, 3191
AcuI	CTGAAG	5 cut(s)	858, 2271, 2426, 2481, 2664
AcyI	GRCGYC	1 cut(s)	78
AfaI	GTAC	2 cut(s)	1452, 2065
AfiI	CCNNNNNNNGG	9 cut(s)	212, 541, 923, 1267, 1322, 2481, 2583, 2617, 2892
AflIII	ACRYGT	1 cut(s)	3060
AjiI	CACGTC	1 cut(s)	373
AjnI	CCWGG	6 cut(s)	741, 1210, 2321, 2545, 2806, 3006
AjuI	GAANNNNNNNTTGG	2 cut(s)	86, 118
AleI	CACNNNNGTG	2 cut(s)	1154, 2158
Alw21I	GWGCWC	2 cut(s)	649, 2165
Alw26I	GTCTC	7 cut(s)	196, 237, 452, 773, 979, 2127, 3348
Alw44I	GTGCAC	1 cut(s)	2161
AlwI	GGATC	9 cut(s)	1014, 1202, 1699, 2537, 2596, 2609, 2874, 3170, 3318
AlwNI	CAGNNNCTG	1 cut(s)	1775
Ama87I	CYCGRG	2 cut(s)	206, 2054
AoxI	GGCC	9 cut(s)	36, 391, 451, 847, 1250, 1728, 2319, 2988, 3034
ApaLI	GTGCAC	1 cut(s)	2161
ApoI	RAATTY	9 cut(s)	785, 992, 1178, 1217, 1629, 1723, 1966, 2672, 3191
ArsI	GACNNNNNNTTYG	2 cut(s)	300, 332
AseI	ATTAAT	2 cut(s)	1371, 1407
Asp700I	GAANNNNTTC	4 cut(s)	1221, 1566, 2376, 3195
AspLEI	GCGC	2 cut(s)	80, 2340
AspS9I	GGNCC	8 cut(s)	37, 391, 421, 878, 1644, 1729, 2665, 3144
AsuC2I	CCSGG	3 cut(s)	207, 208, 2683
AsuHPI	GGTGA	8 cut(s)	97, 154, 1168, 1226, 2321, 2543, 2796, 3124
AsuII	TTCGAA	2 cut(s)	398, 2294
AvaI	CYCGRG	2 cut(s)	206, 2054
AvaII	GGWCC	5 cut(s)	421, 878, 1644, 2665, 3144
AxyI	CCTNAGG	1 cut(s)	2916
BaeGI	GKGCMC	4 cut(s)	1702, 2075, 2165, 2401
BalI	TGGCCA	1 cut(s)	2990
BamHI	GGATCC	1 cut(s)	2601
BanI	GGYRCC	3 cut(s)	77, 1124, 2123
BanII	GRGCYC	1 cut(s)	2212
BarI	GAAGNNNNNNTAC	2 cut(s)	1551, 1583
BauI	CACGAG	1 cut(s)	1114
BbsI	GAAGAC	1 cut(s)	3054
Bbv12I	GWGCWC	2 cut(s)	649, 2165
BceAI	ACGGC	5 cut(s)	367, 1599, 1872, 2923, 2997
BcgI	CGANNNNNNTGC	6 cut(s)	71, 105, 636, 670, 2696, 2730
BciT130I	CCWGG	6 cut(s)	743, 1212, 2323, 2547, 2808, 3008
BciVI	GTATCC	3 cut(s)	1102, 2320, 3430
BclI	TGATCA	3 cut(s)	805, 2020, 2769
BcnI	CCSGG	3 cut(s)	207, 208, 2683
BcoDI	GTCTC	7 cut(s)	196, 237, 452, 773, 979, 2127, 3348
BfmI	CTRYAG	5 cut(s)	960, 1652, 2525, 2739, 3052
BfoI	RGCGCY	1 cut(s)	81
BfuI	GTATCC	3 cut(s)	1102, 2320, 3430
Bme1390I	CCNGG	9 cut(s)	207, 208, 743, 1212, 2323, 2547, 2683, 2808, 3008
Bme18I	GGWCC	5 cut(s)	421, 878, 1644, 2665, 3144
BmeT110I	CYCGRG	2 cut(s)	206, 2054
BmgBI	CACGTC	1 cut(s)	373
BmgT120I	GGNCC	8 cut(s)	37, 391, 421, 878, 1644, 1729, 2665, 3144
BmiI	GGNNCC	9 cut(s)	39, 79, 915, 1126, 1578, 1645, 2125, 2603, 2667
BmrFI	CCNGG	9 cut(s)	207, 208, 743, 1212, 2323, 2547, 2683, 2808, 3008
BmrI	ACTGGG	2 cut(s)	2078, 2626
BmuI	ACTGGG	2 cut(s)	2078, 2626
BoxI	GACNNNNGTC	1 cut(s)	765
BpiI	GAAGAC	1 cut(s)	3054
BplI	GAGNNNNNCTC	6 cut(s)	1185, 1217, 2784, 2816, 2786, 2818
BpmI	CTGGAG	2 cut(s)	2229, 3029
Bpu10I	CCTNAGC	1 cut(s)	2505
Bpu14I	TTCGAA	2 cut(s)	398, 2294
BpuEI	CTTGAG	4 cut(s)	1121, 2113, 2366, 3191
BpuMI	CCSGG	3 cut(s)	207, 208, 2683
BsaBI	GATNNNNATC	3 cut(s)	1005, 1407, 2606
BsaHI	GRCGYC	1 cut(s)	78
BsaI	GGTCTC	2 cut(s)	196, 2127
BsaWI	WCCGGW	3 cut(s)	1582, 2140, 3109
BsaXI	ACNNNNNCTCC	4 cut(s)	45, 75, 207, 237
Bsc4I	CCNNNNNNNGG	9 cut(s)	212, 541, 923, 1267, 1322, 2481, 2583, 2617, 2892
Bse118I	RCCGGY	3 cut(s)	248, 432, 2948
Bse21I	CCTNAGG	1 cut(s)	2916
Bse3DI	GCAATG	4 cut(s)	952, 2388, 2991, 3099
Bse8I	GATNNNNATC	3 cut(s)	1005, 1407, 2606
BseBI	CCWGG	6 cut(s)	743, 1212, 2323, 2547, 2808, 3008
BseJI	GATNNNNATC	3 cut(s)	1005, 1407, 2606
BseLI	CCNNNNNNNGG	9 cut(s)	212, 541, 923, 1267, 1322, 2481, 2583, 2617, 2892
BseMI	GCAATG	4 cut(s)	952, 2388, 2991, 3099
BseRI	GAGGAG	2 cut(s)	255, 1118
BseSI	GKGCMC	4 cut(s)	1702, 2075, 2165, 2401
BseYI	CCCAGC	2 cut(s)	545, 2876
BsgI	GTGCAG	1 cut(s)	164
Bsh1236I	CGCG	3 cut(s)	443, 456, 479
BshFI	GGCC	9 cut(s)	38, 393, 453, 849, 1252, 1730, 2321, 2990, 3036
BshNI	GGYRCC	3 cut(s)	77, 1124, 2123
BsiHKAI	GWGCWC	2 cut(s)	649, 2165
BsiHKCI	CYCGRG	2 cut(s)	206, 2054
BsiSI	CCGG	8 cut(s)	207, 249, 433, 1583, 2141, 2683, 2949, 3110
BslFI	GGGAC	3 cut(s)	224, 2497, 2651
BslI	CCNNNNNNNGG	9 cut(s)	212, 541, 923, 1267, 1322, 2481, 2583, 2617, 2892
BsmAI	GTCTC	7 cut(s)	196, 237, 452, 773, 979, 2127, 3348
BsmBI	CGTCTC	1 cut(s)	452
BsmFI	GGGAC	3 cut(s)	224, 2497, 2651
BsmI	GAATGC	4 cut(s)	2263, 2308, 2340, 2910
BsnI	GGCC	9 cut(s)	38, 393, 453, 849, 1252, 1730, 2321, 2990, 3036
Bso31I	GGTCTC	2 cut(s)	196, 2127
BsoBI	CYCGRG	2 cut(s)	206, 2054
Bsp119I	TTCGAA	2 cut(s)	398, 2294
Bsp1286I	GDGCHC	6 cut(s)	649, 1702, 2075, 2165, 2212, 2401
Bsp19I	CCATGG	1 cut(s)	1461
BspANI	GGCC	9 cut(s)	38, 393, 453, 849, 1252, 1730, 2321, 2990, 3036
BspFNI	CGCG	3 cut(s)	443, 456, 479
BspLI	GGNNCC	9 cut(s)	39, 79, 915, 1126, 1578, 1645, 2125, 2603, 2667
BspPI	GGATC	9 cut(s)	1014, 1202, 1699, 2537, 2596, 2609, 2874, 3170, 3318
BspQI	GCTCTTC	1 cut(s)	479
BspT104I	TTCGAA	2 cut(s)	398, 2294
BspT107I	GGYRCC	3 cut(s)	77, 1124, 2123
BspTNI	GGTCTC	2 cut(s)	196, 2127
BsrBI	CCGCTC	1 cut(s)	3354
BsrDI	GCAATG	4 cut(s)	952, 2388, 2991, 3099
BsrFI	RCCGGY	3 cut(s)	248, 432, 2948
BssAI	RCCGGY	3 cut(s)	248, 432, 2948
BssNI	GRCGYC	1 cut(s)	78
BssSI	CACGAG	1 cut(s)	1114
BssT1I	CCWWGG	2 cut(s)	1461, 2331
Bst2BI	CACGAG	1 cut(s)	1114
Bst2UI	CCWGG	6 cut(s)	743, 1212, 2323, 2547, 2808, 3008
Bst4CI	ACNGT	7 cut(s)	185, 316, 892, 1771, 2529, 3131, 3342
Bst6I	CTCTTC	5 cut(s)	479, 1326, 1790, 1826, 2291
BstACI	GRCGYC	1 cut(s)	78
BstAPI	GCANNNNNTGC	2 cut(s)	187, 2079
BstBI	TTCGAA	2 cut(s)	398, 2294
BstDSI	CCRYGG	4 cut(s)	349, 1461, 1854, 2979
BstEII	GGTNACC	1 cut(s)	2549
BstFNI	CGCG	3 cut(s)	443, 456, 479
BstH2I	RGCGCY	1 cut(s)	81
BstHHI	GCGC	2 cut(s)	80, 2340
BstMAI	GTCTC	7 cut(s)	196, 237, 452, 773, 979, 2127, 3348
BstNI	CCWGG	6 cut(s)	743, 1212, 2323, 2547, 2808, 3008
BstPAI	GACNNNNGTC	1 cut(s)	765
BstPI	GGTNACC	1 cut(s)	2549
BstSCI	CCNGG	9 cut(s)	205, 206, 741, 1210, 2321, 2545, 2681, 2806, 3006
BstSFI	CTRYAG	5 cut(s)	960, 1652, 2525, 2739, 3052
BstSLI	GKGCMC	4 cut(s)	1702, 2075, 2165, 2401
BstUI	CGCG	3 cut(s)	443, 456, 479
BstV2I	GAAGAC	1 cut(s)	3054
BstX2I	RGATCY	5 cut(s)	1006, 1691, 2601, 2866, 3310
BstXI	CCANNNNNNTGG	2 cut(s)	2986, 2998
BstYI	RGATCY	5 cut(s)	1006, 1691, 2601, 2866, 3310
Bsu36I	CCTNAGG	1 cut(s)	2916
BsuI	GTATCC	3 cut(s)	1102, 2320, 3430
BsuRI	GGCC	9 cut(s)	38, 393, 453, 849, 1252, 1730, 2321, 2990, 3036
BtgI	CCRYGG	4 cut(s)	349, 1461, 1854, 2979
BtgZI	GCGATG	1 cut(s)	269
BtrI	CACGTC	1 cut(s)	373
BtsI	GCAGTG	1 cut(s)	2194
CaiI	CAGNNNCTG	1 cut(s)	1775
CfoI	GCGC	2 cut(s)	80, 2340
Cfr10I	RCCGGY	3 cut(s)	248, 432, 2948
Cfr13I	GGNCC	8 cut(s)	37, 391, 421, 878, 1644, 1729, 2665, 3144
Cfr9I	CCCGGG	1 cut(s)	206
CseI	GACGC	3 cut(s)	95, 468, 2321
Csp6I	GTAC	2 cut(s)	1451, 2064
CspCI	CAANNNNNGTGG	2 cut(s)	1102, 1137
CviQI	GTAC	2 cut(s)	1451, 2064
DinI	GGCGCC	1 cut(s)	79
EaeI	YGGCCR	3 cut(s)	451, 1250, 2988
Eam1104I	CTCTTC	5 cut(s)	479, 1326, 1790, 1826, 2291
EarI	CTCTTC	5 cut(s)	479, 1326, 1790, 1826, 2291
EciI	GGCGGA	1 cut(s)	247
Eco130I	CCWWGG	2 cut(s)	1461, 2331
Eco147I	AGGCCT	1 cut(s)	849
Eco24I	GRGCYC	1 cut(s)	2212
Eco31I	GGTCTC	2 cut(s)	196, 2127
Eco32I	GATATC	1 cut(s)	412
Eco47I	GGWCC	5 cut(s)	421, 878, 1644, 2665, 3144
Eco57I	CTGAAG	5 cut(s)	858, 2271, 2426, 2481, 2664
Eco81I	CCTNAGG	1 cut(s)	2916
Eco88I	CYCGRG	2 cut(s)	206, 2054
Eco91I	GGTNACC	1 cut(s)	2549
EcoO109I	RGGNCCY	1 cut(s)	421
EcoO65I	GGTNACC	1 cut(s)	2549
EcoRI	GAATTC	1 cut(s)	785
EcoRII	CCWGG	6 cut(s)	741, 1210, 2321, 2545, 2806, 3006
EcoRV	GATATC	1 cut(s)	412
EcoT14I	CCWWGG	2 cut(s)	1461, 2331
EcoT22I	ATGCAT	1 cut(s)	2263
EcoT38I	GRGCYC	1 cut(s)	2212
EgeI	GGCGCC	1 cut(s)	79
EheI	GGCGCC	1 cut(s)	79
ErhI	CCWWGG	2 cut(s)	1461, 2331
Esp3I	CGTCTC	1 cut(s)	452
FalI	AAGNNNNNCTT	2 cut(s)	714, 746
FaqI	GGGAC	3 cut(s)	224, 2497, 2651
FauI	CCCGC	1 cut(s)	2971
FauNDI	CATATG	2 cut(s)	2862, 3241
FbaI	TGATCA	3 cut(s)	805, 2020, 2769
FriOI	GRGCYC	1 cut(s)	2212
GlaI	GCGC	2 cut(s)	79, 2339
GsaI	CCCAGC	2 cut(s)	549, 2880
GsuI	CTGGAG	2 cut(s)	2229, 3029
HaeII	RGCGCY	1 cut(s)	81
HaeIII	GGCC	9 cut(s)	38, 393, 453, 849, 1252, 1730, 2321, 2990, 3036
HapII	CCGG	8 cut(s)	207, 249, 433, 1583, 2141, 2683, 2949, 3110
HgaI	GACGC	3 cut(s)	95, 468, 2321
HhaI	GCGC	2 cut(s)	80, 2340
Hin1I	GRCGYC	1 cut(s)	78
Hin6I	GCGC	2 cut(s)	78, 2338
HinP1I	GCGC	2 cut(s)	78, 2338
HincII	GTYRAC	3 cut(s)	976, 2923, 3406
HindII	GTYRAC	3 cut(s)	976, 2923, 3406
HindIII	AAGCTT	2 cut(s)	1346, 1506
HpaII	CCGG	8 cut(s)	207, 249, 433, 1583, 2141, 2683, 2949, 3110
HphI	GGTGA	8 cut(s)	97, 154, 1168, 1226, 2321, 2543, 2796, 3124
Hpy166II	GTNNAC	6 cut(s)	888, 976, 2066, 2163, 2923, 3406
Hpy8I	GTNNAC	6 cut(s)	888, 976, 2066, 2163, 2923, 3406
Hpy99I	CGWCG	7 cut(s)	86, 89, 274, 419, 484, 983, 2711
HpyAV	CCTTC	9 cut(s)	56, 361, 434, 569, 835, 1059, 1966, 2833, 3109
HpyCH4III	ACNGT	7 cut(s)	185, 316, 892, 1771, 2529, 3131, 3342
HpyCH4IV	ACGT	2 cut(s)	372, 3062
HpySE526I	ACGT	2 cut(s)	372, 3062
Hsp92I	GRCGYC	1 cut(s)	78
HspAI	GCGC	2 cut(s)	78, 2338
KasI	GGCGCC	1 cut(s)	77
KroI	GCCGGC	2 cut(s)	432, 2948
KroNI	GCCGGC	2 cut(s)	434, 2950
Ksp22I	TGATCA	3 cut(s)	805, 2020, 2769
LguI	GCTCTTC	1 cut(s)	479
LmnI	GCTCC	8 cut(s)	126, 260, 586, 1576, 2335, 3010, 3359, 3396
MaeII	ACGT	2 cut(s)	372, 3062
MbiI	CCGCTC	1 cut(s)	3354
MfeI	CAATTG	1 cut(s)	2967
MflI	RGATCY	5 cut(s)	1006, 1691, 2601, 2866, 3310
MhlI	GDGCHC	6 cut(s)	649, 1702, 2075, 2165, 2212, 2401
MlsI	TGGCCA	1 cut(s)	2990
MluNI	TGGCCA	1 cut(s)	2990
Mly113I	GGCGCC	1 cut(s)	78
MlyI	GAGTC	4 cut(s)	225, 1547, 2066, 2479
MmeI	TCCRAC	1 cut(s)	1669
Mox20I	TGGCCA	1 cut(s)	2990
Mph1103I	ATGCAT	1 cut(s)	2263
MroNI	GCCGGC	2 cut(s)	432, 2948
MroXI	GAANNNNTTC	4 cut(s)	1221, 1566, 2376, 3195
MscI	TGGCCA	1 cut(s)	2990
MslI	CAYNNNNRTG	6 cut(s)	1154, 1705, 2090, 2158, 2984, 3363
Msp20I	TGGCCA	1 cut(s)	2990
MspA1I	CMGCKG	3 cut(s)	1871, 2249, 2443
MspI	CCGG	8 cut(s)	207, 249, 433, 1583, 2141, 2683, 2949, 3110
MspR9I	CCNGG	9 cut(s)	207, 208, 743, 1212, 2323, 2547, 2683, 2808, 3008
MunI	CAATTG	1 cut(s)	2967
Mva1269I	GAATGC	4 cut(s)	2263, 2308, 2340, 2910
MvaI	CCWGG	6 cut(s)	743, 1212, 2323, 2547, 2808, 3008
MvnI	CGCG	3 cut(s)	443, 456, 479
NaeI	GCCGGC	2 cut(s)	434, 2950
NarI	GGCGCC	1 cut(s)	78
NciI	CCSGG	3 cut(s)	207, 208, 2683
NcoI	CCATGG	1 cut(s)	1461
NdeI	CATATG	2 cut(s)	2862, 3241
NgoMIV	GCCGGC	2 cut(s)	432, 2948
NlaIV	GGNNCC	9 cut(s)	39, 79, 915, 1126, 1578, 1645, 2125, 2603, 2667
NmuCI	GTSAC	7 cut(s)	559, 869, 1147, 1771, 2309, 2549, 2701
NsiI	ATGCAT	1 cut(s)	2263
NspV	TTCGAA	2 cut(s)	398, 2294
OliI	CACNNNNGTG	2 cut(s)	1154, 2158
PaeR7I	CTCGAG	1 cut(s)	2054
PceI	AGGCCT	1 cut(s)	849
PciSI	GCTCTTC	1 cut(s)	479
PcsI	WCGNNNNNNNCGW	2 cut(s)	297, 2841
PctI	GAATGC	4 cut(s)	2263, 2308, 2340, 2910
PdiI	GCCGGC	2 cut(s)	434, 2950
PdmI	GAANNNNTTC	4 cut(s)	1221, 1566, 2376, 3195
PfeI	GAWTC	7 cut(s)	308, 502, 1311, 1456, 1562, 1586, 2511
PflFI	GACNNNGTC	1 cut(s)	215
PleI	GAGTC	4 cut(s)	224, 1547, 2065, 2479
PluTI	GGCGCC	1 cut(s)	81
PpsI	GAGTC	4 cut(s)	224, 1547, 2065, 2479
PpuMI	RGGWCCY	1 cut(s)	421
PshAI	GACNNNNGTC	1 cut(s)	765
PshBI	ATTAAT	2 cut(s)	1371, 1407
PsiI	TTATAA	1 cut(s)	3261
Psp5II	RGGWCCY	1 cut(s)	421
Psp6I	CCWGG	6 cut(s)	741, 1210, 2321, 2545, 2806, 3006
PspEI	GGTNACC	1 cut(s)	2549
PspFI	CCCAGC	2 cut(s)	545, 2876
PspGI	CCWGG	6 cut(s)	741, 1210, 2321, 2545, 2806, 3006
PspN4I	GGNNCC	9 cut(s)	39, 79, 915, 1126, 1578, 1645, 2125, 2603, 2667
PspPI	GGNCC	8 cut(s)	37, 391, 421, 878, 1644, 1729, 2665, 3144
PspPPI	RGGWCCY	1 cut(s)	421
PstNI	CAGNNNCTG	1 cut(s)	1775
PsuI	RGATCY	5 cut(s)	1006, 1691, 2601, 2866, 3310
PsyI	GACNNNGTC	1 cut(s)	215
PvuII	CAGCTG	2 cut(s)	2249, 2443
RsaI	GTAC	2 cut(s)	1452, 2065
RsaNI	GTAC	2 cut(s)	1451, 2064
RseI	CAYNNNNRTG	6 cut(s)	1154, 1705, 2090, 2158, 2984, 3363
SapI	GCTCTTC	1 cut(s)	479
Sau96I	GGNCC	8 cut(s)	37, 391, 421, 878, 1644, 1729, 2665, 3144
SchI	GAGTC	4 cut(s)	225, 1547, 2066, 2479
ScrFI	CCNGG	9 cut(s)	207, 208, 743, 1212, 2323, 2547, 2683, 2808, 3008
SduI	GDGCHC	6 cut(s)	649, 1702, 2075, 2165, 2212, 2401
SfcI	CTRYAG	5 cut(s)	960, 1652, 2525, 2739, 3052
SfoI	GGCGCC	1 cut(s)	79
Sfr274I	CTCGAG	1 cut(s)	2054
SfuI	TTCGAA	2 cut(s)	398, 2294
SinI	GGWCC	5 cut(s)	421, 878, 1644, 2665, 3144
SlaI	CTCGAG	1 cut(s)	2054
SmaI	CCCGGG	1 cut(s)	208
SmiMI	CAYNNNNRTG	6 cut(s)	1154, 1705, 2090, 2158, 2984, 3363
SmlI	CTYRAG	5 cut(s)	1100, 2054, 2128, 2345, 3206
SmoI	CTYRAG	5 cut(s)	1100, 2054, 2128, 2345, 3206
SseBI	AGGCCT	1 cut(s)	849
SspDI	GGCGCC	1 cut(s)	77
SspI	AATATT	1 cut(s)	1680
StuI	AGGCCT	1 cut(s)	849
StyD4I	CCNGG	9 cut(s)	205, 206, 741, 1210, 2321, 2545, 2681, 2806, 3006
StyI	CCWWGG	2 cut(s)	1461, 2331
TaaI	ACNGT	7 cut(s)	185, 316, 892, 1771, 2529, 3131, 3342
TaiI	ACGT	2 cut(s)	375, 3065
TaqII	GACCGA	2 cut(s)	2720, 3303
TauI	GCSGC	5 cut(s)	38, 456, 469, 472, 1426
TfiI	GAWTC	7 cut(s)	308, 502, 1311, 1456, 1562, 1586, 2511
TseFI	GTSAC	7 cut(s)	559, 869, 1147, 1771, 2309, 2549, 2701
Tsp45I	GTSAC	7 cut(s)	559, 869, 1147, 1771, 2309, 2549, 2701
TspDTI	ATGAA	6 cut(s)	588, 817, 924, 1389, 1734, 2834
TspGWI	ACGGA	5 cut(s)	228, 1589, 1814, 3045, 3422
TspMI	CCCGGG	1 cut(s)	206
Tth111I	GACNNNGTC	1 cut(s)	215
VneI	GTGCAC	1 cut(s)	2161
VpaK11BI	GGWCC	5 cut(s)	421, 878, 1644, 2665, 3144
VspI	ATTAAT	2 cut(s)	1371, 1407
XapI	RAATTY	9 cut(s)	785, 992, 1178, 1217, 1629, 1723, 1966, 2672, 3191
XcmI	CCANNNNNNNNNTGG	3 cut(s)	2329, 2613, 2983
XhoI	CTCGAG	1 cut(s)	2054
XmaI	CCCGGG	1 cut(s)	206
XmnI	GAANNNNTTC	4 cut(s)	1221, 1566, 2376, 3195
Zsp2I	ATGCAT	1 cut(s)	2263

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.