Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0007030

Physical Location & Seq

Genomic Coordinates Forward (+)

17573792 .. 17579038

Gene Structure 5247 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2322 bp

ATGTCGCGGACCGATAGAATGGGTTCGGATCTTAGCAGGACCGGTCCCGTCGAAAGAGACATCGAGCAGGCTATTACTGCTCTAAAGAAAGGGGCATACTTGCTCAAGTATGGAAGAAGGGGGAAGCCTAAGTTTTGTCCATTTCGTCTTTCCAATGATGAATCCGTTCTAATCTGGTTCTCTGGGAAAGAGGAAAAGCACCTTAAGCTAAGCCACGTGTCTAGAATTATTTCTGGGCAACGGACTCCAATCTTTCAAAGGTATCCACGGCCTGAGAAGGAGTACCAGTCATTTTCTTTAATATATAATGACAGGTCACTAGATTTGATTTGCAAAGATAAAGATGAAGCTGAGGTTTGGTTTAGTGGCTTAAAAGCATTGATATCCCGCAGCCATCATCGAAAATGGAGAACGGAGTCAAGGAGTGATGGAATTCCATCTGAAGCAAATAGTCCTAGAACATACACTCGGAGAAGCTCCCCTCTAAATTCTCCATTTGGTAGTAATGATAGCTTACAGAAGGATAGTGCAGATCACCTTCGCCTTCATAGTCCGTATGAAAGTCCTCCTAAAAATGGTCTGGATAAGGCACTTTCAGATGTGATACTGTATGCTGTTCCTCCCAAGGGTTTCTTCCCTTCAGATTCGGCCAGTGCTTCCGTACATTCTGTGTCATCTGGGGGCTCAGACAGTGTACATGGGCAGATGAAGGCAATGGGAATGGATGCTGTTAGAGTTAGTCTATCCAGTGCTGTTAGCTCATCAAGCCAAGGTTCTGGTCATGATGATGGTGATGCCTTGGGAGATGTTTTCATGTGGGGAGAAGGCACAGGAGATGGTGTTGTGGGTGGTGGTTCTCATAGAGTTGGAAGCTGTCTTGGTGCAAAAATGGATTCTTTGTTGCCCAAAGCCTTAGAATCTAAAGTCGTACTTGACGTCCAAAACATTGCATGTGGTGGTAGGCATGCTGCTTTAGTAACGAAGCAAGGGGAGATTTTCTCTTGGGGAGAGGAATCGGGGGGCAGGCTTGGGCATGGTGTGGATGTTGACGTTTTGCAACCAAAGCTCATTGATGCACTTAGTAATATCAACATTGAGCTTGTTGCTTGTGGTGAGTACCATACTTGTGCTGTTACGCTTTCTGGTGATCTGTACACATGGGGTGATGGAACTTACAATTTCGGTCTACTTGGACATGGAAATGAAGTGAGCCATTGGGTCCCCAAAAGAGTAAATGGACCCTTGGAGGCTATACATGTCTCATCCATATCTTGTGGACCCTGGCACACAGCCGTAGTAACTTCTGCTGGGCAGTTGTTTACTTTTGGTGATGGCACATTTGGTGTTCTTGGCCATGGTGATCGGAAAAGTGTTTCAATTCCCAGGGAAGTGGAATCCCTGAAGGGCCTTCGCACTGTGCGAGCAGCTTGTGGTGTTTGGCATACTGCTGCAGTTGTAGAAGTCATGGTTGGGAATTCAAGTGCCAGCAACTGTTCATTAGGAAAGCTGTTCACTTGGGGAGATGGTGATAAGGGTAGGCTGGGGCATGGTGACAAGGAAGCAAAACTTGTGCCTACCTGTGTTGCCGCTCTGGTTGAATCCAATTTTTGTCGAGTTGCTTGTGGACATAGTATGACAGTTGCTCGTACAACATCTGGTCATGTCTACACAATGGGAAGTCCTGTTTATGGCCAGTTAGGAAATCCTCAAGCTGATGGAAAGCTTCCAACTCGAGTTGAGGGAAAGCTCTCCAAGAGTTTTGTGGAAGAGATTGCTTGTGGTGCCTATCATGTTGCAGTTTTAACTTCAAGAACTGAAGTGTATACTTGGGGAAAAGGAGCAAATGGTCGTTTAGGTCATGGGAATATAGATGATCGAAATTCCCCAACATTAGTTGAAGCTCTGAAAGATAAGCAAGTCAAAAGTATTGCTTGTGGTGCTAATTTTACTGCAGTTATATGCCTTCATAAATGGGTTTCAGGAGTTGATCAGTCAATGTGTTCGGGTTGCCGGCTTCCATTTAATTTTAAAAGAAAACGCCATAATTGTTATAATTGTGGGCTTGTGTTTTGTCATTCTTGCAGCAGTAAGAAATCACTCAAAGCTTCCATGGCACCAAATCCTAGCAAACCTTATCGTGTCTGTGATAATTGTTTTAATAAACTGAGAAAAGCTGCTGAAACTGATACTTCATTACGGACATCTATAAGCAGAAGAGGAAGTATCAATCAGGGGTCAAATGAGGTTATTGATAAAGATGATAAGTTGGACTCCAGATCGATCGTGTGCAACTTGCCAGGTTTTCTTCAATGGAATCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0004842 GO:0005085 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005814 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006303 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0006974 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0015031 GO:0015630 GO:0015833 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0031625 GO:0031974 GO:0031981 GO:0032182 GO:0032183 GO:0032446 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0036211 GO:0042886 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0050896 GO:0051020 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0065009 GO:0070013 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0098772 GO:0140096 GO:1901360 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRX BRX_N BRX_assoc FYVE PH_12 RCC1

Protein Analysis

774

Amino Acids

83.55

Weight (kDa)

8.84

Isoelectric Point (pI)

40.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_12	PF16457	22 - 128	1.2e-09	Pleckstrin homology domain
WD40_RLD	PF25390	269 - 652	2.7e-80	RCC1-like domain
RCC1	PF00415	329 - 378	7e-12	Regulator of chromosome condensation (RCC1) repeat
RCC1_2	PF13540	367 - 392	1.5e-08	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	381 - 433	2.7e-11	Regulator of chromosome condensation (RCC1) repeat
RCC1_2	PF13540	420 - 448	5.3e-06	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	437 - 485	4.8e-14	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	501 - 548	2.8e-13	Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD	PF25384	502 - 602	6.9e-06	Alsin RCC1-like domain
RCC1	PF00415	552 - 601	3.4e-09	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	606 - 653	6.3e-15	Regulator of chromosome condensation (RCC1) repeat
FYVE	PF01363	657 - 723	1.1e-15	FYVE zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007030)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42680
fragaria_vesca	FvH4_2g19022 FvH4_3g28000 FvH4_3g28000 FvH4_3g28000 FvH4_5g11302 FvH4_5g34171 FvH4_6g23262
malus_domestica	MD03G1167700.v1.1 MD11G1186800.v1.1
prunus_persica	Prupe.4G268000_v2.0.a1
pyrus_communis	pycom03g12290 pycom03g12300 pycom11g16130 pycom11g16140
rosa_chinensis	RchiOBHm_Chr5g0050701
rosa_laevigata	RLG00000034724
rosa_multiflora	Rmu_sc0002117.1_g000031
rosa_roxburghii	Rroxscaffold_1G00030080
rosa_rugosa	Rorug05G0263200
rosa_samantha	Rh5AG335200 Rh5BG344400 Rh5CG372500 Rh5DG357600
rosa_wichuraiana	Rw5G031620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2052
AatII	GACGTC	1 cut(s)	939
AccB1I	GGYRCC	2 cut(s)	1781, 2113
AccBSI	CCGCTC	1 cut(s)	1589
AccI	GTMKAC	3 cut(s)	1186, 1665, 1823
AccII	CGCG	1 cut(s)	7
AciI	CCGC	3 cut(s)	7, 388, 1587
AclWI	GGATC	1 cut(s)	36
AcoI	YGGCCR	3 cut(s)	648, 1351, 1690
AcsI	RAATTY	4 cut(s)	432, 487, 1474, 1879
AcuI	CTGAAG	4 cut(s)	462, 624, 1421, 1836
AcvI	CACGTG	1 cut(s)	217
AcyI	GRCGYC	1 cut(s)	936
AfaI	GTAC	7 cut(s)	284, 663, 696, 930, 1118, 1154, 1648
AfiI	CCNNNNNNNGG	3 cut(s)	575, 626, 1688
AflII	CTTAAG	1 cut(s)	203
AflIII	ACRYGT	2 cut(s)	216, 1255
AgeI	ACCGGT	1 cut(s)	41
AgsI	TTSAA	7 cut(s)	257, 1377, 1479, 1598, 1809, 1898, 2309
AjnI	CCWGG	3 cut(s)	1280, 1382, 2296
AjuI	GAANNNNNNNTTGG	4 cut(s)	617, 649, 1452, 1484
Alw26I	GTCTC	2 cut(s)	51, 1264
AlwI	GGATC	1 cut(s)	36
AlwNI	CAGNNNCTG	1 cut(s)	1491
Ama87I	CYCGRG	1 cut(s)	1731
AoxI	GGCC	5 cut(s)	269, 648, 1351, 1405, 1690
ApeKI	GCWGC	6 cut(s)	390, 968, 1424, 1448, 2082, 2174
ApoI	RAATTY	4 cut(s)	432, 487, 1474, 1879
AsiGI	ACCGGT	1 cut(s)	41
Asp700I	GAANNNNTTC	3 cut(s)	22, 165, 229
AspS9I	GGNCC	7 cut(s)	9, 39, 44, 1219, 1238, 1277, 1405
AsuHPI	GGTGA	9 cut(s)	527, 803, 1124, 1157, 1175, 1340, 1370, 1538, 1562
AvaI	CYCGRG	1 cut(s)	1731
AvaII	GGWCC	6 cut(s)	9, 39, 44, 1219, 1238, 1277
BalI	TGGCCA	2 cut(s)	1353, 1692
BanI	GGYRCC	2 cut(s)	1781, 2113
BanII	GRGCYC	1 cut(s)	686
BbrPI	CACGTG	1 cut(s)	217
BbvCI	CCTCAGC	1 cut(s)	351
BbvI	GCAGC	6 cut(s)	402, 955, 1435, 1436, 2094, 2161
BccI	CCATC	9 cut(s)	402, 422, 445, 782, 830, 1160, 1325, 1517, 1709
BceAI	ACGGC	2 cut(s)	284, 1277
BciT130I	CCWGG	3 cut(s)	1282, 1384, 2298
BciVI	GTATCC	1 cut(s)	273
BclI	TGATCA	1 cut(s)	1987
BcoDI	GTCTC	2 cut(s)	51, 1264
BfaI	CTAG	4 cut(s)	222, 320, 456, 2124
BfmI	CTRYAG	2 cut(s)	1449, 1950
BfrI	CTTAAG	1 cut(s)	203
BfuI	GTATCC	1 cut(s)	273
BisI	GCNGC	7 cut(s)	391, 969, 1425, 1449, 1587, 2083, 2175
BlpI	GCTNAGC	1 cut(s)	209
BlsI	GCNGC	7 cut(s)	392, 970, 1426, 1450, 1588, 2084, 2176
Bme1390I	CCNGG	3 cut(s)	1282, 1384, 2298
Bme18I	GGWCC	6 cut(s)	9, 39, 44, 1219, 1238, 1277
BmeT110I	CYCGRG	1 cut(s)	1731
BmgT120I	GGNCC	7 cut(s)	9, 39, 44, 1219, 1238, 1277, 1405
BmiI	GGNNCC	7 cut(s)	46, 1220, 1221, 1240, 1279, 1783, 2115
BmrFI	CCNGG	3 cut(s)	1282, 1384, 2298
BmsI	GCATC	3 cut(s)	715, 784, 1063
BplI	GAGNNNNNCTC	2 cut(s)	983, 1015
BpmI	CTGGAG	1 cut(s)	2257
Bpu10I	CCTNAGC	1 cut(s)	351
Bpu1102I	GCTNAGC	1 cut(s)	209
BpuEI	CTTGAG	2 cut(s)	89, 1692
Bsa29I	ATCGAT	1 cut(s)	2279
BsaAI	YACGTR	1 cut(s)	217
BsaHI	GRCGYC	1 cut(s)	936
BsaWI	WCCGGW	1 cut(s)	41
BsaXI	ACNNNNNCTCC	4 cut(s)	272, 302, 825, 855
Bsc4I	CCNNNNNNNGG	3 cut(s)	575, 626, 1688
Bse118I	RCCGGY	2 cut(s)	41, 2010
Bse1I	ACTGG	4 cut(s)	286, 651, 747, 1693
Bse3DI	GCAATG	2 cut(s)	720, 945
BseBI	CCWGG	3 cut(s)	1282, 1384, 2298
BseCI	ATCGAT	1 cut(s)	2279
BseGI	GGATG	3 cut(s)	730, 1048, 1262
BseLI	CCNNNNNNNGG	3 cut(s)	575, 626, 1688
BseMI	GCAATG	2 cut(s)	720, 945
BseMII	CTCAG	4 cut(s)	264, 342, 699, 2156
BseNI	ACTGG	4 cut(s)	286, 651, 747, 1693
BseXI	GCAGC	6 cut(s)	402, 955, 1435, 1436, 2094, 2161
BseYI	CCCAGC	2 cut(s)	1307, 1540
BsgI	GTGCAG	1 cut(s)	549
Bsh1236I	CGCG	1 cut(s)	7
Bsh1285I	CGRYCG	1 cut(s)	2283
BshFI	GGCC	5 cut(s)	271, 650, 1353, 1407, 1692
BshNI	GGYRCC	2 cut(s)	1781, 2113
BshTI	ACCGGT	1 cut(s)	41
BshVI	ATCGAT	1 cut(s)	2279
BsiEI	CGRYCG	1 cut(s)	2283
BsiHKCI	CYCGRG	1 cut(s)	1731
BsiSI	CCGG	2 cut(s)	42, 2011
BslFI	GGGAC	2 cut(s)	30, 1205
BslI	CCNNNNNNNGG	3 cut(s)	575, 626, 1688
BsmAI	GTCTC	2 cut(s)	51, 1264
BsmFI	GGGAC	2 cut(s)	30, 1205
BsnI	GGCC	5 cut(s)	271, 650, 1353, 1407, 1692
BsoBI	CYCGRG	1 cut(s)	1731
Bsp1286I	GDGCHC	1 cut(s)	686
Bsp1407I	TGTACA	2 cut(s)	694, 1152
Bsp143I	GATC	8 cut(s)	28, 532, 1147, 1360, 1873, 1987, 2276, 2280
Bsp1720I	GCTNAGC	1 cut(s)	209
Bsp19I	CCATGG	2 cut(s)	1354, 2109
BspACI	CCGC	3 cut(s)	7, 388, 1587
BspANI	GGCC	5 cut(s)	271, 650, 1353, 1407, 1692
BspCNI	CTCAG	4 cut(s)	265, 343, 698, 2157
BspDI	ATCGAT	1 cut(s)	2279
BspFNI	CGCG	1 cut(s)	7
BspHI	TCATGA	1 cut(s)	781
BspLI	GGNNCC	7 cut(s)	46, 1220, 1221, 1240, 1279, 1783, 2115
BspMAI	CTGCAG	2 cut(s)	1453, 1954
BspPI	GGATC	1 cut(s)	36
BspT107I	GGYRCC	2 cut(s)	1781, 2113
BspTI	CTTAAG	1 cut(s)	203
BsrBI	CCGCTC	1 cut(s)	1589
BsrDI	GCAATG	2 cut(s)	720, 945
BsrFI	RCCGGY	2 cut(s)	41, 2010
BsrGI	TGTACA	2 cut(s)	694, 1152
BsrI	ACTGG	4 cut(s)	286, 651, 747, 1693
BssAI	RCCGGY	2 cut(s)	41, 2010
BssMI	GATC	8 cut(s)	28, 532, 1147, 1360, 1873, 1987, 2276, 2280
BssNAI	GTATAC	1 cut(s)	1824
BssNI	GRCGYC	1 cut(s)	936
BssT1I	CCWWGG	6 cut(s)	624, 769, 798, 1242, 1354, 2109
Bst1107I	GTATAC	1 cut(s)	1824
Bst2UI	CCWGG	3 cut(s)	1282, 1384, 2298
Bst4CI	ACNGT	5 cut(s)	609, 692, 1417, 1493, 1639
Bst6I	CTCTTC	2 cut(s)	1761, 2209
BstACI	GRCGYC	1 cut(s)	936
BstAFI	CTTAAG	1 cut(s)	203
BstAUI	TGTACA	2 cut(s)	694, 1152
BstBAI	YACGTR	1 cut(s)	217
BstC8I	GCNNGC	6 cut(s)	69, 966, 1025, 1422, 1486, 2012
BstDEI	CTNAG	9 cut(s)	32, 129, 209, 273, 351, 685, 913, 1079, 2165
BstDSI	CCRYGG	3 cut(s)	266, 1354, 2109
BstF5I	GGATG	3 cut(s)	730, 1048, 1262
BstFNI	CGCG	1 cut(s)	7
BstKTI	GATC	8 cut(s)	31, 535, 1150, 1363, 1876, 1990, 2279, 2283
BstMAI	GTCTC	2 cut(s)	51, 1264
BstMBI	GATC	8 cut(s)	28, 532, 1147, 1360, 1873, 1987, 2276, 2280
BstMCI	CGRYCG	1 cut(s)	2283
BstMWI	GCNNNNNNNGC	7 cut(s)	77, 205, 765, 1063, 1781, 1937, 2111
BstNI	CCWGG	3 cut(s)	1282, 1384, 2298
BstNSI	RCATGY	3 cut(s)	954, 968, 1259
BstSCI	CCNGG	3 cut(s)	1280, 1382, 2296
BstSFI	CTRYAG	2 cut(s)	1449, 1950
BstUI	CGCG	1 cut(s)	7
BstV1I	GCAGC	6 cut(s)	402, 955, 1435, 1436, 2094, 2161
BstX2I	RGATCY	1 cut(s)	28
BstXI	CCANNNNNNTGG	2 cut(s)	776, 1390
BstYI	RGATCY	1 cut(s)	28
BstZ17I	GTATAC	1 cut(s)	1824
Bsu15I	ATCGAT	1 cut(s)	2279
BsuI	GTATCC	1 cut(s)	273
BsuRI	GGCC	5 cut(s)	271, 650, 1353, 1407, 1692
BsuTUI	ATCGAT	1 cut(s)	2279
BtgI	CCRYGG	3 cut(s)	266, 1354, 2109
BtsCI	GGATG	3 cut(s)	730, 1048, 1262
BtsIMutI	CAGTG	4 cut(s)	658, 697, 754, 1413
Cac8I	GCNNGC	6 cut(s)	69, 966, 1025, 1422, 1486, 2012
CaiI	CAGNNNCTG	1 cut(s)	1491
CciI	TCATGA	1 cut(s)	781
Cfr10I	RCCGGY	2 cut(s)	41, 2010
Cfr13I	GGNCC	7 cut(s)	9, 39, 44, 1219, 1238, 1277, 1405
ClaI	ATCGAT	1 cut(s)	2279
CpoI	CGGWCCG	1 cut(s)	9
Csp6I	GTAC	7 cut(s)	283, 662, 695, 929, 1117, 1153, 1647
CspAI	ACCGGT	1 cut(s)	41
CspI	CGGWCCG	1 cut(s)	9
CviQI	GTAC	7 cut(s)	283, 662, 695, 929, 1117, 1153, 1647
DdeI	CTNAG	9 cut(s)	32, 129, 209, 273, 351, 685, 913, 1079, 2165
DpnI	GATC	8 cut(s)	30, 534, 1149, 1362, 1875, 1989, 2278, 2282
DpnII	GATC	8 cut(s)	28, 532, 1147, 1360, 1873, 1987, 2276, 2280
DraI	TTTAAA	1 cut(s)	2029
EaeI	YGGCCR	3 cut(s)	648, 1351, 1690
Eam1104I	CTCTTC	2 cut(s)	1761, 2209
EarI	CTCTTC	2 cut(s)	1761, 2209
Eco130I	CCWWGG	6 cut(s)	624, 769, 798, 1242, 1354, 2109
Eco24I	GRGCYC	1 cut(s)	686
Eco32I	GATATC	1 cut(s)	384
Eco47I	GGWCC	6 cut(s)	9, 39, 44, 1219, 1238, 1277
Eco57I	CTGAAG	4 cut(s)	462, 624, 1421, 1836
Eco72I	CACGTG	1 cut(s)	217
Eco88I	CYCGRG	1 cut(s)	1731
EcoO109I	RGGNCCY	2 cut(s)	1219, 1405
EcoRI	GAATTC	2 cut(s)	432, 1474
EcoRII	CCWGG	3 cut(s)	1280, 1382, 2296
EcoRV	GATATC	1 cut(s)	384
EcoT14I	CCWWGG	6 cut(s)	624, 769, 798, 1242, 1354, 2109
EcoT38I	GRGCYC	1 cut(s)	686
ErhI	CCWWGG	6 cut(s)	624, 769, 798, 1242, 1354, 2109
FalI	AAGNNNNNCTT	8 cut(s)	617, 649, 915, 947, 1551, 1583, 1915, 1947
FaqI	GGGAC	2 cut(s)	30, 1205
FauI	CCCGC	1 cut(s)	395
FbaI	TGATCA	1 cut(s)	1987
FblI	GTMKAC	3 cut(s)	1186, 1665, 1823
Fnu4HI	GCNGC	7 cut(s)	391, 969, 1425, 1449, 1587, 2083, 2175
FokI	GGATG	3 cut(s)	737, 1055, 1249
FriOI	GRGCYC	1 cut(s)	686
Fsp4HI	GCNGC	7 cut(s)	391, 969, 1425, 1449, 1587, 2083, 2175
FspBI	CTAG	4 cut(s)	222, 320, 456, 2124
GluI	GCNGC	7 cut(s)	391, 969, 1425, 1449, 1587, 2083, 2175
GsaI	CCCAGC	2 cut(s)	1311, 1544
GsuI	CTGGAG	1 cut(s)	2257
HaeIII	GGCC	5 cut(s)	271, 650, 1353, 1407, 1692
HapII	CCGG	2 cut(s)	42, 2011
Hin1I	GRCGYC	1 cut(s)	936
HincII	GTYRAC	1 cut(s)	1048
HindII	GTYRAC	1 cut(s)	1048
HindIII	AAGCTT	2 cut(s)	1721, 2103
HpaII	CCGG	2 cut(s)	42, 2011
HphI	GGTGA	9 cut(s)	527, 803, 1124, 1157, 1175, 1340, 1370, 1538, 1562
Hpy188I	TCNGA	9 cut(s)	28, 442, 471, 598, 643, 688, 1365, 1905, 2321
Hpy188III	TCNNGA	6 cut(s)	222, 581, 782, 1809, 1980, 2274
Hpy99I	CGWCG	1 cut(s)	53
HpyCH4III	ACNGT	5 cut(s)	609, 692, 1417, 1493, 1639
HpyCH4IV	ACGT	3 cut(s)	216, 936, 1050
HpyF10VI	GCNNNNNNNGC	7 cut(s)	77, 205, 765, 1063, 1781, 1937, 2111
HpyF3I	CTNAG	9 cut(s)	32, 129, 209, 273, 351, 685, 913, 1079, 2165
HpySE526I	ACGT	3 cut(s)	216, 936, 1050
Hsp92I	GRCGYC	1 cut(s)	936
KflI	GGGWCCC	1 cut(s)	1219
KroI	GCCGGC	1 cut(s)	2010
KroNI	GCCGGC	1 cut(s)	2012
Ksp22I	TGATCA	1 cut(s)	1987
Kzo9I	GATC	8 cut(s)	28, 532, 1147, 1360, 1873, 1987, 2276, 2280
LmnI	GCTCC	2 cut(s)	482, 1838
Lsp1109I	GCAGC	6 cut(s)	402, 955, 1435, 1436, 2094, 2161
LweI	GCATC	3 cut(s)	715, 784, 1063
MaeI	CTAG	4 cut(s)	222, 320, 456, 2124
MaeII	ACGT	3 cut(s)	216, 936, 1050
MaeIII	GTNAC	5 cut(s)	315, 976, 1132, 1297, 1550
MalI	GATC	8 cut(s)	30, 534, 1149, 1362, 1875, 1989, 2278, 2282
MbiI	CCGCTC	1 cut(s)	1589
MboI	GATC	8 cut(s)	28, 532, 1147, 1360, 1873, 1987, 2276, 2280
MboII	GAAGA	5 cut(s)	126, 625, 1778, 2226, 2297
MflI	RGATCY	1 cut(s)	28
MhlI	GDGCHC	1 cut(s)	686
MlsI	TGGCCA	2 cut(s)	1353, 1692
MluNI	TGGCCA	2 cut(s)	1353, 1692
MlyI	GAGTC	3 cut(s)	238, 425, 2264
MmeI	TCCRAC	3 cut(s)	847, 1751, 2247
Mox20I	TGGCCA	2 cut(s)	1353, 1692
MroNI	GCCGGC	1 cut(s)	2010
MroXI	GAANNNNTTC	3 cut(s)	22, 165, 229
MscI	TGGCCA	2 cut(s)	1353, 1692
MseI	TTAA	7 cut(s)	204, 299, 371, 1802, 2022, 2028, 2157
MslI	CAYNNNNRTG	3 cut(s)	786, 1125, 1200
Msp20I	TGGCCA	2 cut(s)	1353, 1692
MspCI	CTTAAG	1 cut(s)	203
MspI	CCGG	2 cut(s)	42, 2011
MspR9I	CCNGG	3 cut(s)	1282, 1384, 2298
MvaI	CCWGG	3 cut(s)	1282, 1384, 2298
MvnI	CGCG	1 cut(s)	7
MwoI	GCNNNNNNNGC	7 cut(s)	77, 205, 765, 1063, 1781, 1937, 2111
NaeI	GCCGGC	1 cut(s)	2012
NcoI	CCATGG	2 cut(s)	1354, 2109
NdeII	GATC	8 cut(s)	28, 532, 1147, 1360, 1873, 1987, 2276, 2280
NgoMIV	GCCGGC	1 cut(s)	2010
NlaIV	GGNNCC	7 cut(s)	46, 1220, 1221, 1240, 1279, 1783, 2115
NmuCI	GTSAC	2 cut(s)	315, 1550
NspI	RCATGY	3 cut(s)	954, 968, 1259
PaeI	GCATGC	1 cut(s)	968
PaeR7I	CTCGAG	1 cut(s)	1731
PagI	TCATGA	1 cut(s)	781
PasI	CCCWGGG	1 cut(s)	1383
PciI	ACATGT	1 cut(s)	1255
PcsI	WCGNNNNNNNCGW	2 cut(s)	933, 1417
PdiI	GCCGGC	1 cut(s)	2012
PdmI	GAANNNNTTC	3 cut(s)	22, 165, 229
PfeI	GAWTC	8 cut(s)	161, 644, 893, 917, 1013, 1394, 1598, 2314
PinAI	ACCGGT	1 cut(s)	41
PkrI	GCNGC	7 cut(s)	392, 970, 1426, 1450, 1588, 2084, 2176
Ple19I	CGATCG	1 cut(s)	2283
PleI	GAGTC	3 cut(s)	238, 424, 2264
PmaCI	CACGTG	1 cut(s)	217
PmlI	CACGTG	1 cut(s)	217
PpsI	GAGTC	3 cut(s)	238, 424, 2264
Ppu21I	YACGTR	1 cut(s)	217
PpuMI	RGGWCCY	1 cut(s)	1219
PscI	ACATGT	1 cut(s)	1255
PsiI	TTATAA	1 cut(s)	2052
Psp5II	RGGWCCY	1 cut(s)	1219
Psp6I	CCWGG	3 cut(s)	1280, 1382, 2296
PspCI	CACGTG	1 cut(s)	217
PspFI	CCCAGC	2 cut(s)	1307, 1540
PspGI	CCWGG	3 cut(s)	1280, 1382, 2296
PspN4I	GGNNCC	7 cut(s)	46, 1220, 1221, 1240, 1279, 1783, 2115
PspPI	GGNCC	7 cut(s)	9, 39, 44, 1219, 1238, 1277, 1405
PspPPI	RGGWCCY	1 cut(s)	1219
PspXI	VCTCGAGB	1 cut(s)	1731
PstI	CTGCAG	2 cut(s)	1453, 1954
PstNI	CAGNNNCTG	1 cut(s)	1491
PsuI	RGATCY	1 cut(s)	28
PvuI	CGATCG	1 cut(s)	2283
RsaI	GTAC	7 cut(s)	284, 663, 696, 930, 1118, 1154, 1648
RsaNI	GTAC	7 cut(s)	283, 662, 695, 929, 1117, 1153, 1647
RseI	CAYNNNNRTG	3 cut(s)	786, 1125, 1200
Rsr2I	CGGWCCG	1 cut(s)	9
RsrII	CGGWCCG	1 cut(s)	9
SaqAI	TTAA	7 cut(s)	204, 299, 371, 1802, 2022, 2028, 2157
SatI	GCNGC	7 cut(s)	391, 969, 1425, 1449, 1587, 2083, 2175
Sau3AI	GATC	8 cut(s)	28, 532, 1147, 1360, 1873, 1987, 2276, 2280
Sau96I	GGNCC	7 cut(s)	9, 39, 44, 1219, 1238, 1277, 1405
SchI	GAGTC	3 cut(s)	238, 425, 2264
ScrFI	CCNGG	3 cut(s)	1282, 1384, 2298
SduI	GDGCHC	1 cut(s)	686
SfaNI	GCATC	3 cut(s)	715, 784, 1063
SfcI	CTRYAG	2 cut(s)	1449, 1950
Sfr274I	CTCGAG	1 cut(s)	1731
SinI	GGWCC	6 cut(s)	9, 39, 44, 1219, 1238, 1277
SlaI	CTCGAG	1 cut(s)	1731
SmiMI	CAYNNNNRTG	3 cut(s)	786, 1125, 1200
SmlI	CTYRAG	4 cut(s)	104, 203, 1707, 1731
SmoI	CTYRAG	4 cut(s)	104, 203, 1707, 1731
SphI	GCATGC	1 cut(s)	968
SsiI	CCGC	3 cut(s)	7, 388, 1587
SspMI	CTAG	4 cut(s)	222, 320, 456, 2124
StyD4I	CCNGG	3 cut(s)	1280, 1382, 2296
StyI	CCWWGG	6 cut(s)	624, 769, 798, 1242, 1354, 2109
TaaI	ACNGT	5 cut(s)	609, 692, 1417, 1493, 1639
TaiI	ACGT	3 cut(s)	219, 939, 1053
TaqI	TCGA	7 cut(s)	51, 63, 400, 1612, 1732, 1876, 2279
TaqII	GACCGA	2 cut(s)	26, 1172
TatI	WGTACW	2 cut(s)	694, 1152
TauI	GCSGC	1 cut(s)	1589
TfiI	GAWTC	8 cut(s)	161, 644, 893, 917, 1013, 1394, 1598, 2314
Tru1I	TTAA	7 cut(s)	204, 299, 371, 1802, 2022, 2028, 2157
Tru9I	TTAA	7 cut(s)	204, 299, 371, 1802, 2022, 2028, 2157
TscAI	CASTG	4 cut(s)	658, 697, 754, 1420
TseFI	GTSAC	2 cut(s)	315, 1550
TseI	GCWGC	6 cut(s)	390, 968, 1424, 1448, 2082, 2174
Tsp45I	GTSAC	2 cut(s)	315, 1550
TspGWI	ACGGA	6 cut(s)	154, 256, 428, 543, 649, 2212
TspRI	CASTG	4 cut(s)	658, 697, 754, 1420
Vha464I	CTTAAG	1 cut(s)	203
VpaK11BI	GGWCC	6 cut(s)	9, 39, 44, 1219, 1238, 1277
XapI	RAATTY	4 cut(s)	432, 487, 1474, 1879
XbaI	TCTAGA	1 cut(s)	221
XceI	RCATGY	3 cut(s)	954, 968, 1259
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1759
XhoI	CTCGAG	1 cut(s)	1731
XmiI	GTMKAC	3 cut(s)	1186, 1665, 1823
XmnI	GAANNNNTTC	3 cut(s)	22, 165, 229
XspI	CTAG	4 cut(s)	222, 320, 456, 2124
ZraI	GACGTC	1 cut(s)	937

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.