pycom11g26780

WD repeat-containing protein 89 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009953
Physical Location & Seq
Reverse (-)
29252332 .. 29253781
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 558 bp
ATGGAAGCTACGGACATGGAGGTGGAGGAACTCCCTCCGTCCAATCCTGACCCCTTCAAGCGTATCTCCCTAAAAAATTCCATCCAAACCAACTTCGGCGACGACTATGTCTTCCAAATTGCCCCCAAGGATGATTGGACGGCAATGGCGGTGTCGCTTTCGACCAACGCCGTGAAGGTGTACTCTCCGGTGACGGGCCAGTACTACGGAGAGTGCAAGGGCCACTCTGCCACTATCAACCAAATTGCATTTTCAGGTCCATCAACTCCACATGTCCTGCACTCTTGTTCTTCTGATGGAACGATTCGAGCTTGGGACACGCGCACATTTCAACAGGTTTCATCTTTTCATTCTGGCTCTTCTCAGGAGATTTTCAGCTTTTCGTTCGGAGGATCAGGGAACAATCTTCTCGCCGCGGGATGTGATGCTCAGATACTATTCTGGGATTGGAGGAGCAATAAACAAGTAGCGTGCCTTGAGGATTCTCATGTAGAAGATGTTACCCAGGTTCACTTCATCCCGGATCGATCAAAGCAAGCTTCTTTCTGCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010029 GO:0040008 GO:0048580 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

186

Amino Acids

20.43

Weight (kDa)

4.94

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 28 - 176 7.6e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 34 - 148 2.5e-06 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 36 - 116 2.1e-06 WDHD1 first WD40 domain
WDR55 PF24796 37 - 162 4e-07 WDR55
Beta-prop_WDR5 PF25175 46 - 177 4.7e-20 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 52 - 179 1.8e-14 WDR3 first beta-propeller domain
Beta-prop_WDR75_1st PF23869 52 - 107 3e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_THOC3 PF25174 54 - 177 3.3e-16 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 56 - 149 3.7e-10 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 57 - 175 6.4e-14 Prp19 WD40 domain
WD40_Gbeta PF25391 95 - 176 1.9e-06 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 95 - 178 3.5e-06 Echinoderm microtubule-associated protein first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AccII CGCG 2 cut(s) 322, 416
AciI CCGC 3 cut(s) 149, 414, 416
AclWI GGATC 2 cut(s) 400, 531
AcsI RAATTY 1 cut(s) 76
AfaI GTAC 2 cut(s) 182, 203
AfiI CCNNNNNNNGG 1 cut(s) 194
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 2 cut(s) 58, 332
AjnI CCWGG 1 cut(s) 504
AluBI AGCT 4 cut(s) 8, 311, 378, 539
AluI AGCT 4 cut(s) 8, 311, 378, 539
AlwI GGATC 2 cut(s) 400, 531
AoxI GGCC 2 cut(s) 196, 220
ApoI RAATTY 1 cut(s) 76
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 3 cut(s) 196, 220, 257
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 1 cut(s) 202
AvaII GGWCC 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 103
BccI CCATC 3 cut(s) 89, 268, 290
BceAI ACGGC 2 cut(s) 155, 156
BciT130I CCWGG 1 cut(s) 506
BcnI CCSGG 1 cut(s) 521
BisI GCNGC 1 cut(s) 414
BlsI GCNGC 1 cut(s) 415
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 2 cut(s) 506, 521
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 3 cut(s) 196, 220, 257
BmrFI CCNGG 2 cut(s) 506, 521
BmsI GCATC 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 521
Bsa29I ATCGAT 1 cut(s) 526
BsaJI CCNNGG 3 cut(s) 126, 414, 504
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 506
BseCI ATCGAT 1 cut(s) 526
BseDI CCNNGG 3 cut(s) 126, 414, 504
BseGI GGATG 4 cut(s) 81, 136, 425, 516
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 2 cut(s) 377, 443
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 1 cut(s) 466
BsgI GTGCAG 1 cut(s) 263
Bsh1236I CGCG 2 cut(s) 322, 416
BshFI GGCC 2 cut(s) 198, 222
BshVI ATCGAT 1 cut(s) 526
BsiSI CCGG 2 cut(s) 188, 521
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 329
BsnI GGCC 2 cut(s) 198, 222
Bsp143I GATC 3 cut(s) 392, 523, 527
BspACI CCGC 3 cut(s) 149, 414, 416
BspANI GGCC 2 cut(s) 198, 222
BspCNI CTCAG 2 cut(s) 376, 442
BspDI ATCGAT 1 cut(s) 526
BspFNI CGCG 2 cut(s) 322, 416
BspPI GGATC 2 cut(s) 400, 531
BspQI GCTCTTC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 150
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 3 cut(s) 126, 414, 504
BssMI GATC 3 cut(s) 392, 523, 527
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 1 cut(s) 506
Bst6I CTCTTC 1 cut(s) 364
BstC8I GCNNGC 2 cut(s) 472, 537
BstDEI CTNAG 2 cut(s) 363, 429
BstDSI CCRYGG 1 cut(s) 414
BstF5I GGATG 4 cut(s) 81, 136, 425, 516
BstFNI CGCG 2 cut(s) 322, 416
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 3 cut(s) 395, 526, 530
BstMBI GATC 3 cut(s) 392, 523, 527
BstNI CCWGG 1 cut(s) 506
BstNSI RCATGY 1 cut(s) 275
BstSCI CCNGG 2 cut(s) 504, 519
BstUI CGCG 2 cut(s) 322, 416
BstV2I GAAGAC 1 cut(s) 103
Bsu15I ATCGAT 1 cut(s) 526
BsuRI GGCC 2 cut(s) 198, 222
BsuTUI ATCGAT 1 cut(s) 526
BtgI CCRYGG 1 cut(s) 414
BtsCI GGATG 4 cut(s) 81, 136, 425, 516
Cac8I GCNNGC 2 cut(s) 472, 537
CfoI GCGC 1 cut(s) 324
Cfr13I GGNCC 3 cut(s) 196, 220, 257
Cfr42I CCGCGG 1 cut(s) 417
ClaI ATCGAT 1 cut(s) 526
Csp6I GTAC 2 cut(s) 181, 202
CviAII CATG 3 cut(s) 16, 272, 488
CviJI RGCY 7 cut(s) 8, 198, 222, 311, 357, 378, 539
CviKI_1 RGCY 7 cut(s) 8, 198, 222, 311, 357, 378, 539
CviQI GTAC 2 cut(s) 181, 202
DdeI CTNAG 2 cut(s) 363, 429
DpnI GATC 3 cut(s) 394, 525, 529
DpnII GATC 3 cut(s) 392, 523, 527
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
Eam1104I CTCTTC 1 cut(s) 364
EarI CTCTTC 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 504
EcoT14I CCWWGG 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 3 cut(s) 19, 275, 491
FaiI YATR 4 cut(s) 17, 108, 273, 489
FaqI GGGAC 1 cut(s) 329
FatI CATG 3 cut(s) 15, 271, 487
FauI CCCGC 1 cut(s) 409
Fnu4HI GCNGC 1 cut(s) 414
FokI GGATG 4 cut(s) 68, 143, 432, 503
Fsp4HI GCNGC 1 cut(s) 414
GlaI GCGC 1 cut(s) 323
GluI GCNGC 1 cut(s) 414
HaeIII GGCC 2 cut(s) 198, 222
HapII CCGG 2 cut(s) 188, 521
HhaI GCGC 1 cut(s) 324
Hin1II CATG 3 cut(s) 19, 275, 491
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 537
HinfI GANTC 2 cut(s) 304, 482
HpaII CCGG 2 cut(s) 188, 521
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 2 cut(s) 181, 511
Hpy188I TCNGA 3 cut(s) 295, 389, 432
Hpy188III TCNNGA 2 cut(s) 47, 365
Hpy8I GTNNAC 2 cut(s) 181, 511
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 2 cut(s) 64, 169
HpyCH4V TGCA 3 cut(s) 216, 248, 280
HpyF3I CTNAG 2 cut(s) 363, 429
Hsp92II CATG 3 cut(s) 19, 275, 491
HspAI GCGC 1 cut(s) 322
KspI CCGCGG 1 cut(s) 417
Kzo9I GATC 3 cut(s) 392, 523, 527
LguI GCTCTTC 1 cut(s) 364
LmnI GCTCC 1 cut(s) 453
LweI GCATC 1 cut(s) 415
MaeIII GTNAC 2 cut(s) 190, 499
MalI GATC 3 cut(s) 394, 525, 529
MboI GATC 3 cut(s) 392, 523, 527
MboII GAAGA 5 cut(s) 103, 282, 351, 398, 506
MluCI AATT 3 cut(s) 76, 117, 243
MnlI CCTC 6 cut(s) 13, 19, 45, 383, 444, 472
MslI CAYNNNNRTG 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 2 cut(s) 188, 521
MspR9I CCNGG 2 cut(s) 506, 521
MvaI CCWGG 1 cut(s) 506
MvnI CGCG 2 cut(s) 322, 416
NciI CCSGG 1 cut(s) 521
NdeII GATC 3 cut(s) 392, 523, 527
NlaIII CATG 3 cut(s) 19, 275, 491
NmuCI GTSAC 1 cut(s) 190
NspI RCATGY 1 cut(s) 275
PciI ACATGT 1 cut(s) 271
PciSI GCTCTTC 1 cut(s) 364
PfeI GAWTC 2 cut(s) 304, 482
PflFI GACNNNGTC 1 cut(s) 107
PfoI TCCNGGA 1 cut(s) 519
PkrI GCNGC 1 cut(s) 415
PscI ACATGT 1 cut(s) 271
Psp6I CCWGG 1 cut(s) 504
PspGI CCWGG 1 cut(s) 504
PspPI GGNCC 3 cut(s) 196, 220, 257
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 2 cut(s) 182, 203
RsaNI GTAC 2 cut(s) 181, 202
RseI CAYNNNNRTG 1 cut(s) 20
SacII CCGCGG 1 cut(s) 417
SapI GCTCTTC 1 cut(s) 364
SatI GCNGC 1 cut(s) 414
Sau3AI GATC 3 cut(s) 392, 523, 527
Sau96I GGNCC 3 cut(s) 196, 220, 257
ScaI AGTACT 1 cut(s) 203
ScrFI CCNGG 2 cut(s) 506, 521
SetI ASST 9 cut(s) 10, 24, 180, 259, 313, 339, 380, 510, 541
SfaNI GCATC 1 cut(s) 415
Sfr303I CCGCGG 1 cut(s) 417
SgrBI CCGCGG 1 cut(s) 417
SinI GGWCC 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 3 cut(s) 76, 117, 243
SsiI CCGC 3 cut(s) 149, 414, 416
StyD4I CCNGG 2 cut(s) 504, 519
StyI CCWWGG 1 cut(s) 126
TaqI TCGA 3 cut(s) 161, 307, 526
TasI AATT 3 cut(s) 76, 117, 243
TatI WGTACW 2 cut(s) 180, 201
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 2 cut(s) 304, 482
TseFI GTSAC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 3 cut(s) 330, 338, 505
TspGWI ACGGA 3 cut(s) 26, 27, 222
Tth111I GACNNNGTC 1 cut(s) 107
VpaK11BI GGWCC 1 cut(s) 257
XapI RAATTY 1 cut(s) 76
XceI RCATGY 1 cut(s) 275
ZrmI AGTACT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.