pycom11g27400

Glutamyl endopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002809
Physical Location & Seq
Reverse (-)
29666003 .. 29667350
1348 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1086 bp
ATGAATGTCGACAGGATGCTCCACAAAGTCTATCATCTTCTCTCCCTCACGTCTCCTCACTCTCTCCCTTTCAAGCCCCTCCACTTCCCATCGGTCCTTTCACTCTCCCACCTCAGACCAAGGCGCTCCCCATACGATTCCGTAAGGCCCATCAGAACATCCACCGCCGTCATGGCCACCTCTCGCCTCCGCAACCTCGTCCCCGCCAACAATTTCGCTTCCGAGGATGCCAGCGGTGGCGGAGCCTCCAAAGGCTCTGTCTCTTCCACCGCTGCTACTACTGCACTTGATGATGAAGAAGATGCAACTCTGGCAGTTAGATACCGTCTTCCTCCGCAAGAAATTAAAGATATTGTGGATGCTCCGCCACTTCCCGCCTTGTCATTCTCGCCGTCTAGGGATAAGATACTGTTTCTCAAGCGGAGAGCTTTGCCGCCATTGACAGAACTTGCAAGACCTGAGGAAAAGCTTGCCGGTGTTCGTATTGATGGAAAATGCAATACAAGGACCAGGATGTCGTTTTACTCGGGTATTGGGATCCATCAATTATTGCCCGATGGCACCCTTGGTCCCGAAATAGAGGTGCGTGGATTCCCTGATGGTGCCAAGATCAATTTTGTTACTTGGTCCCCGGATGGCCGCCATTTAGCCTTCACCATCCGGTTTGATGAGGGGGAAACTAGTACTAGTAAGCTTAGAGTATGGGTGGCCCAAGTTGAAACAGGGATTGCTAGACCTTTGTTTAAGTCTGAAGAAATATTTTTGAATGCTATGTTTGACAATTTTGTTTGGGTGAATGACTCCTCCTTGGTAGTTTGCACCATTCCCCAGTCTCGTGGAGACCCGCCAAAGAAACCTTTGGTTCCCTTTGGGCCTAAAATTCAATCTAACGAGCAGAAAAACACTATCCAAGTTAGGACCTTTCAGGATTTGCTGAAGGATGAGTATGATGAAGATTTGTTTGACTACTACACCACATCACAACTTGTTTTGGCCTCCTTGGATGGGGCGGTGAAGGAAATTGGTCCTCCAGCTGTATATACATCAATGAACCCCTCTCCCGATCGATCGAGAAATACCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

362

Amino Acids

40.11

Weight (kDa)

7.78

Isoelectric Point (pI)

54.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 514
AccB1I GGYRCC 2 cut(s) 560, 602
AccI GTMKAC 1 cut(s) 9
AclWI GGATC 2 cut(s) 532, 545
AcoI YGGCCR 2 cut(s) 174, 637
AcsI RAATTY 1 cut(s) 879
AcuI CTGAAG 2 cut(s) 771, 956
AfaI GTAC 1 cut(s) 685
AfiI CCNNNNNNNGG 1 cut(s) 1005
AgsI TTSAA 4 cut(s) 73, 719, 766, 884
AhlI ACTAGT 2 cut(s) 680, 686
AjiI CACGTC 1 cut(s) 51
AjnI CCWGG 1 cut(s) 509
AluBI AGCT 4 cut(s) 428, 469, 694, 1034
AluI AGCT 4 cut(s) 428, 469, 694, 1034
Alw26I GTCTC 4 cut(s) 57, 265, 834, 837
AlwI GGATC 2 cut(s) 532, 545
Ama87I CYCGRG 1 cut(s) 526
AoxI GGCC 6 cut(s) 146, 174, 637, 708, 872, 993
ApeKI GCWGC 1 cut(s) 272
ApoI RAATTY 1 cut(s) 879
ArsI GACNNNNNNTTYG 2 cut(s) 243, 275
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 9 cut(s) 94, 147, 507, 569, 627, 709, 872, 918, 1025
AsuC2I CCSGG 1 cut(s) 632
AsuHPI GGTGA 3 cut(s) 646, 805, 1024
AvaI CYCGRG 1 cut(s) 526
AvaII GGWCC 6 cut(s) 94, 507, 569, 627, 918, 1025
AxyI CCTNAGG 1 cut(s) 459
BalI TGGCCA 1 cut(s) 176
BamHI GGATCC 1 cut(s) 537
BanI GGYRCC 2 cut(s) 560, 602
BauI CACGAG 1 cut(s) 834
BbsI GAAGAC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 259
BccI CCATC 9 cut(s) 97, 158, 482, 549, 551, 593, 629, 665, 998
BceAI ACGGC 2 cut(s) 152, 376
BciT130I CCWGG 1 cut(s) 511
BcnI CCSGG 1 cut(s) 632
BcoDI GTCTC 4 cut(s) 57, 265, 834, 837
BcuI ACTAGT 2 cut(s) 680, 686
BfaI CTAG 4 cut(s) 396, 681, 687, 732
BfoI RGCGCY 1 cut(s) 127
BglI GCCNNNNNGGC 1 cut(s) 173
BisI GCNGC 3 cut(s) 273, 434, 640
BlsI GCNGC 3 cut(s) 274, 435, 641
BmcAI AGTACT 1 cut(s) 685
Bme1390I CCNGG 2 cut(s) 511, 632
Bme18I GGWCC 6 cut(s) 94, 507, 569, 627, 918, 1025
BmeT110I CYCGRG 1 cut(s) 526
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 9 cut(s) 94, 147, 507, 569, 627, 709, 872, 918, 1025
BmiI GGNNCC 7 cut(s) 244, 539, 562, 571, 604, 629, 864
BmrFI CCNGG 2 cut(s) 511, 632
BmrI ACTGGG 1 cut(s) 823
BmsI GCATC 4 cut(s) 6, 217, 292, 349
BmuI ACTGGG 1 cut(s) 823
BpiI GAAGAC 1 cut(s) 320
BpmI CTGGAG 1 cut(s) 1014
BpuEI CTTGAG 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 632
Bsa29I ATCGAT 1 cut(s) 1066
BsaI GGTCTC 1 cut(s) 834
BsaJI CCNNGG 6 cut(s) 119, 222, 565, 630, 807, 999
BsaWI WCCGGW 1 cut(s) 660
Bsc4I CCNNNNNNNGG 1 cut(s) 1005
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 1 cut(s) 829
Bse21I CCTNAGG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 511
BseCI ATCGAT 1 cut(s) 1066
BseDI CCNNGG 6 cut(s) 119, 222, 565, 630, 807, 999
BseGI GGATG 9 cut(s) 21, 158, 232, 364, 519, 640, 657, 946, 1009
BseLI CCNNNNNNNGG 1 cut(s) 1005
BseMII CTCAG 2 cut(s) 127, 450
BseNI ACTGG 1 cut(s) 829
BseRI GAGGAG 2 cut(s) 45, 793
BseXI GCAGC 1 cut(s) 259
BsgI GTGCAG 1 cut(s) 267
Bsh1285I CGRYCG 2 cut(s) 1066, 1070
BshFI GGCC 6 cut(s) 148, 176, 639, 710, 874, 995
BshNI GGYRCC 2 cut(s) 560, 602
BshVI ATCGAT 1 cut(s) 1066
BsiEI CGRYCG 2 cut(s) 1066, 1070
BsiHKCI CYCGRG 1 cut(s) 526
BsiSI CCGG 3 cut(s) 474, 632, 661
BslFI GGGAC 3 cut(s) 185, 555, 613
BslI CCNNNNNNNGG 1 cut(s) 1005
BsmAI GTCTC 4 cut(s) 57, 265, 834, 837
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 3 cut(s) 185, 555, 613
BsmI GAATGC 1 cut(s) 772
BsnI GGCC 6 cut(s) 148, 176, 639, 710, 874, 995
Bso31I GGTCTC 1 cut(s) 834
BsoBI CYCGRG 1 cut(s) 526
Bsp143I GATC 4 cut(s) 537, 609, 1063, 1067
BspANI GGCC 6 cut(s) 148, 176, 639, 710, 874, 995
BspCNI CTCAG 2 cut(s) 126, 451
BspDI ATCGAT 1 cut(s) 1066
BspLI GGNNCC 7 cut(s) 244, 539, 562, 571, 604, 629, 864
BspPI GGATC 2 cut(s) 532, 545
BspT107I GGYRCC 2 cut(s) 560, 602
BspTNI GGTCTC 1 cut(s) 834
BsrFI RCCGGY 1 cut(s) 473
BsrI ACTGG 1 cut(s) 829
BssAI RCCGGY 1 cut(s) 473
BssECI CCNNGG 6 cut(s) 119, 222, 565, 630, 807, 999
BssMI GATC 4 cut(s) 537, 609, 1063, 1067
BssSI CACGAG 1 cut(s) 834
BssT1I CCWWGG 4 cut(s) 119, 565, 807, 999
Bst2BI CACGAG 1 cut(s) 834
Bst2UI CCWGG 1 cut(s) 511
Bst4CI ACNGT 2 cut(s) 326, 411
Bst6I CTCTTC 1 cut(s) 268
BstC8I GCNNGC 2 cut(s) 232, 471
BstDEI CTNAG 3 cut(s) 113, 459, 695
BstF5I GGATG 9 cut(s) 21, 158, 232, 364, 519, 640, 657, 946, 1009
BstH2I RGCGCY 1 cut(s) 127
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 4 cut(s) 540, 612, 1066, 1070
BstMAI GTCTC 4 cut(s) 57, 265, 834, 837
BstMBI GATC 4 cut(s) 537, 609, 1063, 1067
BstMCI CGRYCG 2 cut(s) 1066, 1070
BstMWI GCNNNNNNNGC 3 cut(s) 173, 281, 311
BstNI CCWGG 1 cut(s) 511
BstSCI CCNGG 2 cut(s) 509, 630
BstV1I GCAGC 1 cut(s) 259
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 537
BstXI CCANNNNNNTGG 1 cut(s) 836
BstYI RGATCY 1 cut(s) 537
Bsu15I ATCGAT 1 cut(s) 1066
Bsu36I CCTNAGG 1 cut(s) 459
BsuRI GGCC 6 cut(s) 148, 176, 639, 710, 874, 995
BsuTUI ATCGAT 1 cut(s) 1066
BtrI CACGTC 1 cut(s) 51
BtsCI GGATG 9 cut(s) 21, 158, 232, 364, 519, 640, 657, 946, 1009
Cac8I GCNNGC 2 cut(s) 232, 471
CfoI GCGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 473
Cfr13I GGNCC 9 cut(s) 94, 147, 507, 569, 627, 709, 872, 918, 1025
ClaI ATCGAT 1 cut(s) 1066
Csp6I GTAC 1 cut(s) 684
CviAII CATG 1 cut(s) 172
CviQI GTAC 1 cut(s) 684
DdeI CTNAG 3 cut(s) 113, 459, 695
DpnI GATC 4 cut(s) 539, 611, 1065, 1069
DpnII GATC 4 cut(s) 537, 609, 1063, 1067
DrdI GACNNNNNNGTC 1 cut(s) 514
DseDI GACNNNNNNGTC 1 cut(s) 514
EaeI YGGCCR 2 cut(s) 174, 637
Eam1104I CTCTTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 268
EciI GGCGGA 2 cut(s) 255, 354
Eco130I CCWWGG 4 cut(s) 119, 565, 807, 999
Eco31I GGTCTC 1 cut(s) 834
Eco47I GGWCC 6 cut(s) 94, 507, 569, 627, 918, 1025
Eco57I CTGAAG 2 cut(s) 771, 956
Eco81I CCTNAGG 1 cut(s) 459
Eco88I CYCGRG 1 cut(s) 526
EcoO109I RGGNCCY 1 cut(s) 918
EcoRII CCWGG 1 cut(s) 509
EcoT14I CCWWGG 4 cut(s) 119, 565, 807, 999
ErhI CCWWGG 4 cut(s) 119, 565, 807, 999
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 1 cut(s) 175
FaiI YATR 7 cut(s) 133, 173, 703, 773, 948, 1039, 1041
FaqI GGGAC 3 cut(s) 185, 555, 613
FatI CATG 1 cut(s) 171
FauI CCCGC 3 cut(s) 211, 382, 852
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 3 cut(s) 273, 434, 640
FokI GGATG 9 cut(s) 28, 145, 239, 371, 526, 644, 647, 953, 1016
Fsp4HI GCNGC 3 cut(s) 273, 434, 640
FspBI CTAG 4 cut(s) 396, 681, 687, 732
GlaI GCGC 1 cut(s) 125
GluI GCNGC 3 cut(s) 273, 434, 640
GsuI CTGGAG 1 cut(s) 1014
HaeII RGCGCY 1 cut(s) 127
HaeIII GGCC 6 cut(s) 148, 176, 639, 710, 874, 995
HapII CCGG 3 cut(s) 474, 632, 661
HhaI GCGC 1 cut(s) 126
Hin1II CATG 1 cut(s) 175
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HindIII AAGCTT 2 cut(s) 467, 692
HinfI GANTC 3 cut(s) 137, 591, 800
HpaII CCGG 3 cut(s) 474, 632, 661
HphI GGTGA 3 cut(s) 646, 805, 1024
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 4 cut(s) 116, 155, 223, 751
Hpy188III TCNNGA 4 cut(s) 572, 926, 1061, 1071
Hpy8I GTNNAC 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 661, 931, 1009
HpyCH4III ACNGT 2 cut(s) 326, 411
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 5 cut(s) 284, 305, 452, 498, 819
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 281, 311
HpyF3I CTNAG 3 cut(s) 113, 459, 695
HpySE526I ACGT 1 cut(s) 50
Hsp92II CATG 1 cut(s) 175
HspAI GCGC 1 cut(s) 124
Kzo9I GATC 4 cut(s) 537, 609, 1063, 1067
LmnI GCTCC 4 cut(s) 24, 131, 242, 367
Lsp1109I GCAGC 1 cut(s) 259
LweI GCATC 4 cut(s) 6, 217, 292, 349
MaeI CTAG 4 cut(s) 396, 681, 687, 732
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 619
MalI GATC 4 cut(s) 539, 611, 1065, 1069
MboI GATC 4 cut(s) 537, 609, 1063, 1067
MboII GAAGA 7 cut(s) 29, 255, 308, 311, 320, 764, 965
MflI RGATCY 1 cut(s) 537
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 7 cut(s) 211, 342, 545, 613, 781, 879, 1020
MluNI TGGCCA 1 cut(s) 176
MlyI GAGTC 1 cut(s) 794
Mox20I TGGCCA 1 cut(s) 176
MscI TGGCCA 1 cut(s) 176
MseI TTAA 3 cut(s) 345, 744, 1084
Msp20I TGGCCA 1 cut(s) 176
MspA1I CMGCKG 3 cut(s) 234, 272, 1034
MspI CCGG 3 cut(s) 474, 632, 661
MspR9I CCNGG 2 cut(s) 511, 632
Mva1269I GAATGC 1 cut(s) 772
MvaI CCWGG 1 cut(s) 511
MwoI GCNNNNNNNGC 3 cut(s) 173, 281, 311
NciI CCSGG 1 cut(s) 632
NdeII GATC 4 cut(s) 537, 609, 1063, 1067
NlaIII CATG 1 cut(s) 175
NlaIV GGNNCC 7 cut(s) 244, 539, 562, 571, 604, 629, 864
PctI GAATGC 1 cut(s) 772
PfeI GAWTC 2 cut(s) 137, 591
PkrI GCNGC 3 cut(s) 274, 435, 641
Ple19I CGATCG 2 cut(s) 1066, 1070
PleI GAGTC 1 cut(s) 794
PpsI GAGTC 1 cut(s) 794
PpuMI RGGWCCY 1 cut(s) 918
Psp5II RGGWCCY 1 cut(s) 918
Psp6I CCWGG 1 cut(s) 509
PspGI CCWGG 1 cut(s) 509
PspN4I GGNNCC 7 cut(s) 244, 539, 562, 571, 604, 629, 864
PspPI GGNCC 9 cut(s) 94, 147, 507, 569, 627, 709, 872, 918, 1025
PspPPI RGGWCCY 1 cut(s) 918
PsuI RGATCY 1 cut(s) 537
PvuI CGATCG 2 cut(s) 1066, 1070
PvuII CAGCTG 1 cut(s) 1034
RsaI GTAC 1 cut(s) 685
RsaNI GTAC 1 cut(s) 684
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 3 cut(s) 345, 744, 1084
SatI GCNGC 3 cut(s) 273, 434, 640
Sau3AI GATC 4 cut(s) 537, 609, 1063, 1067
Sau96I GGNCC 9 cut(s) 94, 147, 507, 569, 627, 709, 872, 918, 1025
ScaI AGTACT 1 cut(s) 685
SchI GAGTC 1 cut(s) 794
ScrFI CCNGG 2 cut(s) 511, 632
SfaNI GCATC 4 cut(s) 6, 217, 292, 349
SinI GGWCC 6 cut(s) 94, 507, 569, 627, 918, 1025
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
SpeI ACTAGT 2 cut(s) 680, 686
Sse9I AATT 7 cut(s) 211, 342, 545, 613, 781, 879, 1020
SspI AATATT 1 cut(s) 759
SspMI CTAG 4 cut(s) 396, 681, 687, 732
StyD4I CCNGG 2 cut(s) 509, 630
StyI CCWWGG 4 cut(s) 119, 565, 807, 999
TaaI ACNGT 2 cut(s) 326, 411
TaiI ACGT 1 cut(s) 53
TaqI TCGA 3 cut(s) 9, 1066, 1070
TaqII GACCGA 1 cut(s) 82
TasI AATT 7 cut(s) 211, 342, 545, 613, 781, 879, 1020
TatI WGTACW 1 cut(s) 683
TauI GCSGC 2 cut(s) 436, 642
TfiI GAWTC 2 cut(s) 137, 591
Tru1I TTAA 3 cut(s) 345, 744, 1084
Tru9I TTAA 3 cut(s) 345, 744, 1084
TseI GCWGC 1 cut(s) 272
TspDTI ATGAA 4 cut(s) 17, 309, 966, 1064
TspGWI ACGGA 1 cut(s) 130
VpaK11BI GGWCC 6 cut(s) 94, 507, 569, 627, 918, 1025
XapI RAATTY 1 cut(s) 879
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 4 cut(s) 396, 681, 687, 732
ZrmI AGTACT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.