pycom12433g00050

protein C9orf85 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012433
OG0006795
Physical Location & Seq
Reverse (-)
25528 .. 26331
804 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
ATGATGAACGGCGGTCGGCACCAAAACAAATTCGCATGGAAGCCCAACCTCGGCGTCAAGATCGACGAAACCGAGGTCGGAGGCCGGTTCCGGCCGCTGTCGGATATCACGGGGGTCTGCCAACGCTGCAAGGAACAGATCGAGTGGAAGCGCCGTTACGGAAAGTACAAGACCCTGACGGAGCCCGCCAAGTGCCAACGCTGTACCAAACGCGCCGTCCGGCAGTCCCACCATAAGCTCTGCGCTGCTTGCGCCAAGGAACAAGGTGCTTGTGCCAAGTGCTGCTGCCGTGTCGAGCAAATCGTCGGAAAAGACCTCGCGGAAGTGGAGGCTGAGCAGAAGAAGCTTCAAGAGGCGATAAAAAATGCAAGAGAAAGAGATAGGAGGAGTCTGTTGCGTGCGATGAATGCTGGAAAATCTGCGGGTGTAGCGAAAGCTGCAGGCGAGAAAGGAGGCAAAGCGGGGGAGTTGTTCCCCTCCGTGTCGCTTGAAGAATATGCAGCGGCAACCAGAGACGATGATGATGATGTGGAGGAGGATGGTGAAGGTGAAGGTGAAGAAGATGAGACGCATGTTTGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2039

Protein Analysis

195

Amino Acids

21.53

Weight (kDa)

7.97

Isoelectric Point (pI)

49.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2039 PF10217 6 - 96 1.7e-26 Uncharacterized conserved protein (DUF2039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 18
AccII CGCG 2 cut(s) 213, 320
AciI CCGC 7 cut(s) 12, 95, 186, 320, 422, 461, 503
AcoI YGGCCR 1 cut(s) 92
AcsI RAATTY 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 167, 205
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 2 cut(s) 350, 491
AluBI AGCT 3 cut(s) 238, 346, 437
AluI AGCT 3 cut(s) 238, 346, 437
Alw26I GTCTC 2 cut(s) 507, 560
AoxI GGCC 2 cut(s) 82, 92
ApeKI GCWGC 6 cut(s) 126, 245, 282, 285, 437, 500
ApoI RAATTY 1 cut(s) 29
ArsI GACNNNNNNTTYG 4 cut(s) 60, 92, 201, 233
AspLEI GCGC 4 cut(s) 153, 215, 245, 254
AsuHPI GGTGA 3 cut(s) 554, 560, 566
BanI GGYRCC 1 cut(s) 18
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BbvI GCAGC 6 cut(s) 113, 232, 269, 272, 424, 512
BccI CCATC 1 cut(s) 533
BceAI ACGGC 4 cut(s) 25, 138, 200, 273
BcoDI GTCTC 2 cut(s) 507, 560
BfmI CTRYAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 154
BisI GCNGC 8 cut(s) 95, 127, 246, 283, 286, 438, 501, 504
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 8 cut(s) 96, 128, 247, 284, 287, 439, 502, 505
BmiI GGNNCC 3 cut(s) 20, 89, 183
Bpu1102I GCTNAGC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 49, 72, 255
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse118I RCCGGY 1 cut(s) 84
BseDI CCNNGG 3 cut(s) 49, 72, 255
BseGI GGATG 1 cut(s) 544
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 1 cut(s) 324
BseRI GAGGAG 2 cut(s) 400, 548
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 6 cut(s) 113, 232, 269, 272, 424, 512
Bsh1236I CGCG 2 cut(s) 213, 320
Bsh1285I CGRYCG 2 cut(s) 16, 95
BshFI GGCC 2 cut(s) 84, 94
BshNI GGYRCC 1 cut(s) 18
BsiEI CGRYCG 2 cut(s) 16, 95
BsiSI CCGG 3 cut(s) 85, 91, 220
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 2 cut(s) 507, 560
BsmBI CGTCTC 2 cut(s) 507, 560
BsmFI GGGAC 1 cut(s) 211
BsmI GAATGC 1 cut(s) 412
BsnI GGCC 2 cut(s) 84, 94
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 2 cut(s) 60, 138
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 7 cut(s) 12, 95, 186, 320, 422, 461, 503
BspANI GGCC 2 cut(s) 84, 94
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 2 cut(s) 213, 320
BspLI GGNNCC 3 cut(s) 20, 89, 183
BspMAI CTGCAG 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 84
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 3 cut(s) 49, 72, 255
BssMI GATC 2 cut(s) 60, 138
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 255
BstACI GRCGYC 1 cut(s) 54
BstC8I GCNNGC 4 cut(s) 186, 250, 399, 442
BstDEI CTNAG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 544
BstFNI CGCG 2 cut(s) 213, 320
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 4 cut(s) 153, 215, 245, 254
BstKTI GATC 2 cut(s) 63, 141
BstMAI GTCTC 2 cut(s) 507, 560
BstMBI GATC 2 cut(s) 60, 138
BstMCI CGRYCG 2 cut(s) 16, 95
BstMWI GCNNNNNNNGC 7 cut(s) 126, 249, 251, 343, 407, 428, 437
BstNSI RCATGY 1 cut(s) 575
BstSFI CTRYAG 1 cut(s) 438
BstUI CGCG 2 cut(s) 213, 320
BstV1I GCAGC 6 cut(s) 113, 232, 269, 272, 424, 512
BstZI CGGCCG 1 cut(s) 92
BsuRI GGCC 2 cut(s) 84, 94
BtgZI GCGATG 1 cut(s) 416
BtsCI GGATG 1 cut(s) 544
Cac8I GCNNGC 4 cut(s) 186, 250, 399, 442
CfoI GCGC 4 cut(s) 153, 215, 245, 254
Cfr10I RCCGGY 1 cut(s) 84
CseI GACGC 2 cut(s) 43, 577
Csp6I GTAC 2 cut(s) 166, 204
CviAII CATG 2 cut(s) 36, 572
CviJI RGCY 8 cut(s) 43, 84, 94, 184, 238, 332, 346, 437
CviKI_1 RGCY 8 cut(s) 43, 84, 94, 184, 238, 332, 346, 437
CviQI GTAC 2 cut(s) 166, 204
DdeI CTNAG 1 cut(s) 333
DpnI GATC 2 cut(s) 62, 140
DpnII GATC 2 cut(s) 60, 138
EaeI YGGCCR 1 cut(s) 92
EagI CGGCCG 1 cut(s) 92
EclXI CGGCCG 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 186
Eco32I GATATC 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 92
EcoRV GATATC 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 255
Esp3I CGTCTC 2 cut(s) 507, 560
FaeI CATG 2 cut(s) 39, 575
FaiI YATR 4 cut(s) 37, 234, 498, 573
FaqI GGGAC 1 cut(s) 211
FatI CATG 2 cut(s) 35, 571
FauI CCCGC 3 cut(s) 193, 415, 454
Fnu4HI GCNGC 8 cut(s) 95, 127, 246, 283, 286, 438, 501, 504
FokI GGATG 1 cut(s) 551
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 8 cut(s) 95, 127, 246, 283, 286, 438, 501, 504
GlaI GCGC 4 cut(s) 152, 214, 244, 253
GluI GCNGC 8 cut(s) 95, 127, 246, 283, 286, 438, 501, 504
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 2 cut(s) 84, 94
HapII CCGG 3 cut(s) 85, 91, 220
HgaI GACGC 2 cut(s) 43, 577
HhaI GCGC 4 cut(s) 153, 215, 245, 254
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 2 cut(s) 39, 575
Hin6I GCGC 4 cut(s) 151, 213, 243, 252
HinP1I GCGC 4 cut(s) 151, 213, 243, 252
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 1 cut(s) 388
HpaII CCGG 3 cut(s) 85, 91, 220
HphI GGTGA 3 cut(s) 554, 560, 566
Hpy188I TCNGA 3 cut(s) 80, 103, 308
Hpy188III TCNNGA 2 cut(s) 58, 350
Hpy99I CGWCG 2 cut(s) 68, 308
HpyAV CCTTC 2 cut(s) 539, 545
HpyCH4V TGCA 5 cut(s) 129, 368, 440, 500, 579
HpyF10VI GCNNNNNNNGC 7 cut(s) 126, 249, 251, 343, 407, 428, 437
HpyF3I CTNAG 1 cut(s) 333
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 2 cut(s) 39, 575
HspAI GCGC 4 cut(s) 151, 213, 243, 252
Kzo9I GATC 2 cut(s) 60, 138
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 7 cut(s) 98, 104, 188, 233, 396, 426, 523
Lsp1109I GCAGC 6 cut(s) 113, 232, 269, 272, 424, 512
MaeIII GTNAC 1 cut(s) 155
MalI GATC 2 cut(s) 62, 140
MboI GATC 2 cut(s) 60, 138
MboII GAAGA 4 cut(s) 352, 503, 569, 572
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 2 cut(s) 29, 580
MlyI GAGTC 1 cut(s) 397
MmeI TCCRAC 3 cut(s) 58, 81, 286
MseI TTAA 1 cut(s) 583
MspA1I CMGCKG 2 cut(s) 97, 503
MspI CCGG 3 cut(s) 85, 91, 220
Mva1269I GAATGC 1 cut(s) 412
MvnI CGCG 2 cut(s) 213, 320
MwoI GCNNNNNNNGC 7 cut(s) 126, 249, 251, 343, 407, 428, 437
NdeII GATC 2 cut(s) 60, 138
NlaIII CATG 2 cut(s) 39, 575
NlaIV GGNNCC 3 cut(s) 20, 89, 183
NmeAIII GCCGAG 1 cut(s) 30
NspI RCATGY 1 cut(s) 575
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 300
PctI GAATGC 1 cut(s) 412
PkrI GCNGC 8 cut(s) 96, 128, 247, 284, 287, 439, 502, 505
PleI GAGTC 1 cut(s) 396
PpsI GAGTC 1 cut(s) 396
PspN4I GGNNCC 3 cut(s) 20, 89, 183
PstI CTGCAG 1 cut(s) 442
RsaI GTAC 2 cut(s) 167, 205
RsaNI GTAC 2 cut(s) 166, 204
SaqAI TTAA 1 cut(s) 583
SatI GCNGC 8 cut(s) 95, 127, 246, 283, 286, 438, 501, 504
Sau3AI GATC 2 cut(s) 60, 138
SchI GAGTC 1 cut(s) 397
SduI GDGCHC 1 cut(s) 186
SetI ASST 9 cut(s) 51, 78, 240, 268, 318, 348, 439, 550, 556
SfcI CTRYAG 1 cut(s) 438
Sse9I AATT 2 cut(s) 29, 580
SsiI CCGC 7 cut(s) 12, 95, 186, 320, 422, 461, 503
StyI CCWWGG 1 cut(s) 255
TaqI TCGA 3 cut(s) 63, 141, 294
TasI AATT 2 cut(s) 29, 580
TatI WGTACW 1 cut(s) 165
TauI GCSGC 2 cut(s) 97, 506
Tru1I TTAA 1 cut(s) 583
Tru9I TTAA 1 cut(s) 583
TseI GCWGC 6 cut(s) 126, 245, 282, 285, 437, 500
TspDTI ATGAA 2 cut(s) 20, 419
TspGWI ACGGA 3 cut(s) 174, 194, 469
XapI RAATTY 1 cut(s) 29
XceI RCATGY 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.