pycom12g05080

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009153
Physical Location & Seq
Reverse (-)
4951588 .. 4955722
4135 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
ATGAACACCGCCATGAAAGCGGCTCTCACAATCTCCAATCCCAACTGCTGCATTTATGTGAGGGCCGCCCTCTTTCATTCCACCCCTGTCTTGGATCGTAAGCGACGATCCTACTCAGAACCTAGATTTAACAACTATGGAAGAAGGTCCAGAAGGTTCCATGGAAAGCAAACTTTAACGCGCAATGTCAATGCTTATGCAGACTTCTTATTTCAGAGTTGGCAGAATGAGTATGATGATGAGGAGCCATCTTCAAGCAGAGGCACCTCATGGTTTAAAAAGCAATATTCCGCCAAGGGGTCCAAAATGAAGGCAGGCAATCAAGGAGCCTGGAAATACGGAAGAAGTGATTTCCGGTTTTGTGAAGAAGATGAAGACGTGGAGACCATTTTCCGATCCATGTTTGGTGGAAACAAATTTTATTATTGGTCATTCATTAATGAAGAGAATTCACAGTGGAGGAGCTCTAGTAATTACTCTAACTATGGGAGGTCTTGGAATTGGAGACATAACAATAAGGAAGAGTATCAATATGAATCTGAGCCTGAGAATATGGAGTCAGATGAAAGGTTGGCCCTCGGGTTGCATGCTTCGGGTCCTCTAACACTTGATGATGTCAAGAATGCGTATCGAATCTGTGCGCTGAAATGGCATCCAGATCGTCACCAGGGTTCTTCCAAGGCAGCTGCAGAGGAAAAGTTCAAGCTTTGCAGTGCAGCATATCAATCATTATACTGTAAACTGGCTGCGGAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

253

Amino Acids

29.47

Weight (kDa)

8.89

Isoelectric Point (pI)

55.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 201 - 244 5.7e-10 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 263
AccII CGCG 1 cut(s) 181
AciI CCGC 5 cut(s) 9, 20, 66, 291, 749
AclWI GGATC 3 cut(s) 102, 102, 390
AcsI RAATTY 2 cut(s) 416, 448
AfiI CCNNNNNNNGG 2 cut(s) 91, 297
AgsI TTSAA 2 cut(s) 255, 703
AjiI CACGTC 1 cut(s) 379
AjnI CCWGG 2 cut(s) 329, 666
AluBI AGCT 3 cut(s) 465, 686, 706
AluI AGCT 3 cut(s) 465, 686, 706
Alw21I GWGCWC 1 cut(s) 467
Alw26I GTCTC 2 cut(s) 377, 499
AlwI GGATC 3 cut(s) 102, 102, 390
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 2 cut(s) 63, 573
ApeKI GCWGC 5 cut(s) 48, 683, 686, 716, 746
ApoI RAATTY 2 cut(s) 416, 448
AseI ATTAAT 1 cut(s) 438
AspLEI GCGC 2 cut(s) 183, 643
AspS9I GGNCC 5 cut(s) 63, 147, 300, 574, 596
AsuHPI GGTGA 1 cut(s) 656
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 3 cut(s) 147, 300, 596
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 467
BbvI GCAGC 5 cut(s) 35, 673, 695, 728, 733
BccI CCATC 1 cut(s) 256
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BciT130I CCWGG 2 cut(s) 331, 668
BcoDI GTCTC 2 cut(s) 377, 499
BfaI CTAG 2 cut(s) 123, 468
BfmI CTRYAG 1 cut(s) 687
BisI GCNGC 7 cut(s) 21, 49, 66, 684, 687, 717, 747
BlsI GCNGC 7 cut(s) 22, 50, 67, 685, 688, 718, 748
Bme1390I CCNGG 2 cut(s) 331, 668
Bme18I GGWCC 3 cut(s) 147, 300, 596
BmeT110I CYCGRG 1 cut(s) 578
BmgBI CACGTC 1 cut(s) 379
BmgT120I GGNCC 5 cut(s) 63, 147, 300, 574, 596
BmiI GGNNCC 6 cut(s) 158, 246, 265, 301, 328, 597
BmrFI CCNGG 2 cut(s) 331, 668
BmsI GCATC 1 cut(s) 661
BpiI GAAGAC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 5 cut(s) 160, 294, 577, 667, 678
BsaWI WCCGGW 1 cut(s) 354
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 297
Bse1I ACTGG 1 cut(s) 747
Bse3DI GCAATG 1 cut(s) 190
BseBI CCWGG 2 cut(s) 331, 668
BseDI CCNNGG 5 cut(s) 160, 294, 577, 667, 678
BseGI GGATG 1 cut(s) 652
BseLI CCNNNNNNNGG 2 cut(s) 91, 297
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 3 cut(s) 129, 531, 537
BseNI ACTGG 1 cut(s) 747
BseRI GAGGAG 2 cut(s) 257, 475
BseXI GCAGC 5 cut(s) 35, 673, 695, 728, 733
BsgI GTGCAG 1 cut(s) 735
Bsh1236I CGCG 1 cut(s) 181
BshFI GGCC 2 cut(s) 65, 575
BshNI GGYRCC 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 467
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 1 cut(s) 355
BslI CCNNNNNNNGG 2 cut(s) 91, 297
BsmAI GTCTC 2 cut(s) 377, 499
BsmI GAATGC 1 cut(s) 628
BsnI GGCC 2 cut(s) 65, 575
Bso31I GGTCTC 1 cut(s) 377
BsoBI CYCGRG 1 cut(s) 578
Bsp1286I GDGCHC 1 cut(s) 467
Bsp143I GATC 4 cut(s) 94, 107, 395, 658
Bsp19I CCATGG 1 cut(s) 160
BspACI CCGC 5 cut(s) 9, 20, 66, 291, 749
BspANI GGCC 2 cut(s) 65, 575
BspCNI CTCAG 3 cut(s) 128, 532, 538
BspFNI CGCG 1 cut(s) 181
BspLI GGNNCC 6 cut(s) 158, 246, 265, 301, 328, 597
BspMAI CTGCAG 1 cut(s) 691
BspPI GGATC 3 cut(s) 102, 102, 390
BspT107I GGYRCC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 190
BsrI ACTGG 1 cut(s) 747
BssECI CCNNGG 5 cut(s) 160, 294, 577, 667, 678
BssMI GATC 4 cut(s) 94, 107, 395, 658
BssT1I CCWWGG 3 cut(s) 160, 294, 678
Bst2UI CCWGG 2 cut(s) 331, 668
Bst4CI ACNGT 2 cut(s) 456, 737
Bst6I CTCTTC 2 cut(s) 438, 516
BstC8I GCNNGC 2 cut(s) 316, 588
BstDEI CTNAG 3 cut(s) 115, 540, 546
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 1 cut(s) 652
BstFNI CGCG 1 cut(s) 181
BstHHI GCGC 2 cut(s) 183, 643
BstKTI GATC 4 cut(s) 97, 110, 398, 661
BstMAI GTCTC 2 cut(s) 377, 499
BstMBI GATC 4 cut(s) 94, 107, 395, 658
BstMWI GCNNNNNNNGC 2 cut(s) 17, 649
BstNI CCWGG 2 cut(s) 331, 668
BstNSI RCATGY 1 cut(s) 590
BstSCI CCNGG 2 cut(s) 329, 666
BstSFI CTRYAG 1 cut(s) 687
BstUI CGCG 1 cut(s) 181
BstV1I GCAGC 5 cut(s) 35, 673, 695, 728, 733
BstV2I GAAGAC 1 cut(s) 381
BsuRI GGCC 2 cut(s) 65, 575
BtgI CCRYGG 1 cut(s) 160
BtrI CACGTC 1 cut(s) 379
BtsCI GGATG 1 cut(s) 652
BtsI GCAGTG 1 cut(s) 718
BtsIMutI CAGTG 2 cut(s) 461, 718
Cac8I GCNNGC 2 cut(s) 316, 588
CfoI GCGC 2 cut(s) 183, 643
Cfr13I GGNCC 5 cut(s) 63, 147, 300, 574, 596
CviAII CATG 5 cut(s) 13, 161, 270, 400, 587
DdeI CTNAG 3 cut(s) 115, 540, 546
DpnI GATC 4 cut(s) 96, 109, 397, 660
DpnII GATC 4 cut(s) 94, 107, 395, 658
DraI TTTAAA 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 438, 516
EarI CTCTTC 2 cut(s) 438, 516
EciI GGCGGA 1 cut(s) 280
Ecl136II GAGCTC 1 cut(s) 465
Eco130I CCWWGG 3 cut(s) 160, 294, 678
Eco24I GRGCYC 1 cut(s) 467
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 3 cut(s) 147, 300, 596
Eco53kI GAGCTC 1 cut(s) 465
Eco88I CYCGRG 1 cut(s) 578
EcoICRI GAGCTC 1 cut(s) 465
EcoO109I RGGNCCY 1 cut(s) 596
EcoRI GAATTC 1 cut(s) 448
EcoRII CCWGG 2 cut(s) 329, 666
EcoT14I CCWWGG 3 cut(s) 160, 294, 678
EcoT38I GRGCYC 1 cut(s) 467
ErhI CCWWGG 3 cut(s) 160, 294, 678
FaeI CATG 5 cut(s) 16, 164, 273, 403, 590
FatI CATG 5 cut(s) 12, 160, 269, 399, 586
Fnu4HI GCNGC 7 cut(s) 21, 49, 66, 684, 687, 717, 747
FokI GGATG 1 cut(s) 639
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 7 cut(s) 21, 49, 66, 684, 687, 717, 747
FspBI CTAG 2 cut(s) 123, 468
GlaI GCGC 2 cut(s) 182, 642
GluI GCNGC 7 cut(s) 21, 49, 66, 684, 687, 717, 747
HaeIII GGCC 2 cut(s) 65, 575
HapII CCGG 1 cut(s) 355
HhaI GCGC 2 cut(s) 183, 643
Hin1II CATG 5 cut(s) 16, 164, 273, 403, 590
Hin6I GCGC 2 cut(s) 181, 641
HinP1I GCGC 2 cut(s) 181, 641
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 3 cut(s) 536, 557, 633
HpaII CCGG 1 cut(s) 355
HphI GGTGA 1 cut(s) 656
Hpy166II GTNNAC 1 cut(s) 740
Hpy188I TCNGA 5 cut(s) 118, 216, 395, 541, 562
Hpy188III TCNNGA 3 cut(s) 150, 619, 656
Hpy8I GTNNAC 1 cut(s) 740
Hpy99I CGWCG 1 cut(s) 108
HpyAV CCTTC 3 cut(s) 138, 147, 304
HpyCH4III ACNGT 2 cut(s) 456, 737
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 6 cut(s) 51, 200, 586, 689, 711, 716
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 649
HpyF3I CTNAG 3 cut(s) 115, 540, 546
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 5 cut(s) 16, 164, 273, 403, 590
HspAI GCGC 2 cut(s) 181, 641
Kzo9I GATC 4 cut(s) 94, 107, 395, 658
LmnI GCTCC 3 cut(s) 244, 326, 462
Lsp1109I GCAGC 5 cut(s) 35, 673, 695, 728, 733
LweI GCATC 1 cut(s) 661
MaeI CTAG 2 cut(s) 123, 468
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 662
MalI GATC 4 cut(s) 96, 109, 397, 660
MboI GATC 4 cut(s) 94, 107, 395, 658
MboII GAAGA 9 cut(s) 153, 243, 354, 377, 380, 386, 455, 533, 666
MhlI GDGCHC 1 cut(s) 467
MluCI AATT 4 cut(s) 416, 448, 472, 499
MlyI GAGTC 1 cut(s) 566
MseI TTAA 4 cut(s) 129, 176, 276, 438
MslI CAYNNNNRTG 2 cut(s) 11, 56
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 1 cut(s) 355
MspR9I CCNGG 2 cut(s) 331, 668
Mva1269I GAATGC 1 cut(s) 628
MvaI CCWGG 2 cut(s) 331, 668
MvnI CGCG 1 cut(s) 181
MwoI GCNNNNNNNGC 2 cut(s) 17, 649
NcoI CCATGG 1 cut(s) 160
NdeII GATC 4 cut(s) 94, 107, 395, 658
NlaIII CATG 5 cut(s) 16, 164, 273, 403, 590
NlaIV GGNNCC 6 cut(s) 158, 246, 265, 301, 328, 597
NmuCI GTSAC 1 cut(s) 662
NspI RCATGY 1 cut(s) 590
PaeI GCATGC 1 cut(s) 590
PcsI WCGNNNNNNNCGW 1 cut(s) 103
PctI GAATGC 1 cut(s) 628
PfeI GAWTC 2 cut(s) 536, 633
PkrI GCNGC 7 cut(s) 22, 50, 67, 685, 688, 718, 748
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
PpuMI RGGWCCY 1 cut(s) 596
PshBI ATTAAT 1 cut(s) 438
Psp124BI GAGCTC 1 cut(s) 467
Psp5II RGGWCCY 1 cut(s) 596
Psp6I CCWGG 2 cut(s) 329, 666
PspGI CCWGG 2 cut(s) 329, 666
PspN4I GGNNCC 6 cut(s) 158, 246, 265, 301, 328, 597
PspPI GGNCC 5 cut(s) 63, 147, 300, 574, 596
PspPPI RGGWCCY 1 cut(s) 596
PstI CTGCAG 1 cut(s) 691
PvuII CAGCTG 1 cut(s) 686
RseI CAYNNNNRTG 2 cut(s) 11, 56
SacI GAGCTC 1 cut(s) 467
SaqAI TTAA 4 cut(s) 129, 176, 276, 438
SatI GCNGC 7 cut(s) 21, 49, 66, 684, 687, 717, 747
Sau3AI GATC 4 cut(s) 94, 107, 395, 658
Sau96I GGNCC 5 cut(s) 63, 147, 300, 574, 596
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 2 cut(s) 331, 668
SduI GDGCHC 1 cut(s) 467
SfaNI GCATC 1 cut(s) 661
SfcI CTRYAG 1 cut(s) 687
SinI GGWCC 3 cut(s) 147, 300, 596
SmiMI CAYNNNNRTG 2 cut(s) 11, 56
SphI GCATGC 1 cut(s) 590
Sse9I AATT 4 cut(s) 416, 448, 472, 499
SsiI CCGC 5 cut(s) 9, 20, 66, 291, 749
SspI AATATT 1 cut(s) 287
SspMI CTAG 2 cut(s) 123, 468
SstI GAGCTC 1 cut(s) 467
StyD4I CCNGG 2 cut(s) 329, 666
StyI CCWWGG 3 cut(s) 160, 294, 678
TaaI ACNGT 2 cut(s) 456, 737
TaiI ACGT 1 cut(s) 381
TaqI TCGA 1 cut(s) 631
TasI AATT 4 cut(s) 416, 448, 472, 499
TauI GCSGC 2 cut(s) 23, 68
TfiI GAWTC 2 cut(s) 536, 633
Tru1I TTAA 4 cut(s) 129, 176, 276, 438
Tru9I TTAA 4 cut(s) 129, 176, 276, 438
TscAI CASTG 2 cut(s) 461, 718
TseFI GTSAC 1 cut(s) 662
TseI GCWGC 5 cut(s) 48, 683, 686, 716, 746
Tsp45I GTSAC 1 cut(s) 662
TspDTI ATGAA 9 cut(s) 17, 29, 65, 323, 387, 424, 456, 549, 579
TspGWI ACGGA 1 cut(s) 354
TspRI CASTG 2 cut(s) 461, 718
VpaK11BI GGWCC 3 cut(s) 147, 300, 596
VspI ATTAAT 1 cut(s) 438
XapI RAATTY 2 cut(s) 416, 448
XceI RCATGY 1 cut(s) 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 2 cut(s) 123, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.