pycom12g06760

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002172
Physical Location & Seq
Forward (+)
7059759 .. 7065418
5660 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2502 bp
ATGCCCACAACAGCAATACATTACAAGTGTCCTCTACTAATTGGAGGCAGCAGCACATATCTTTTGCGCGTACATGTACGGAATCTTCGTCGTCCGTCCTATCCACAAAAAGAAGAGAGCTTAACGTGTGGAAATGGAGAAGTTCTTGAAGGACTTCGAGGTGGAGAACAAGAATCCATCGGAGGAGGCTATAAGGAGATGGAGAAACGCCGTCGCGCTCGTCAAAAACCCTCGCAGAAGGTTTCGATTCGTCGCCGATCGATCGCCAAGCGCTCCGAGGCCGAAAAGAAGAAGCTTCAAATCCAGGAAAAGATTCGAGTTGCTCTTTATGTTCAAAAAGCAGCACTGCATTTCATTGATGCTGGTGATCGTGGATCTATTGAAAAGCTAGGTCAGGATGAACTCAAGCTGTCAGAAGATGCCAGAATGGCAGGTTTCAGCATTCACCCAGATGAACTTGCATCTATTACACGTGCCCATGATATTAAGGCCTTGGAAAGCCATGGTGGAATTCATGGGATTTTAAGGAAAGTCAATGTCTCGGTAGATGAAGGTGTCAAGGATAGTAATATACCAATCAGACAAAATGTTTATGGCTTAAACCGTTACAAGGAGAAACCTCCCCGAACTTTTTGGGTGTTTGTATGGGAAGCACTACAGGACTTGACATTAATAATCCTTATGGTCTGTGCTGTGGTTTCAATTGGAGTTGGAATTGCCACTGAAGGCTGGCCCAAAGGCACGTACGATGGTCTGGGAATTTTAATTAGTATAATTCTAGTGGTTATGGTTACCGCCATTAGTGACTACAAGCAGTCTTTGCAATTCCAGGATTTGGACAGGGAGAAGAAAAAGATTTTTGTTCAGGTCACTAGGGATGGAAAAAGACAAAAAGTTTCCATTTACGACTTGGTTGTTGGAGATATTGTGCATTTGTCGATTGGGGACCAAGTTCCAGCTGATGGACTTTTCATATCTGGGTACAGTTTGCTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCGGTGAATGTATCTGAAGAGAAGCCTTTTCTTCTTTCCGGAACTAAAGTGCAGGATGGGTCAGGTAAAATGCTAGCGACTACAGTTGGTATGAGGACTGAATGGGGAAAGTTGATGGAAACTCTGAGTGAAGGAGGAGAAGATGAGACCCCACTGCAGGTGAAGCTTAATGGTGTTGCTACAGTTATTGGTAAAATTGGTTTGACTTTTGCAGTGTTGACATTTTTGGTATTGACAGTAAGATTTTTGGTGACAAAAGGACTTAACAACGAGATCACTGACTGGTCTTCAACTGACGCCGTAACCCTTTTGAACTACTTTGCTATTGCGGTAACTATAATTGTTGTTGCAGTTCCCGAAGGATTACCATTAGCAGTGACGCTGAGCCTTGCTTTTGCTATGAAAAAATTGATGAATGATAGGGCACTTGTAAGGCATCTCTCAGCATGTGAGACAATGGGTTCTGCTAGTTGTATTTGCACAGATAAAACTGGAACATTAACTACAAATCATATGGTAGTTACCAAAGTATGGATATGTGAAAAATCTGTAGATGTAAAAGAAAATGACAGTAAAGAAAGGTTGATATCAGAAATATCTGGAGCATCAAGCATCCTTTTGCAGGTTATATTCCAAAATACAAGTTCTGAGGTTATTAAGGATGATGGAAAGACCTCCATTTTGGGAACACCAACAGAGTCAGCACTATTAGAGTTTGGTTTACTTTTAGGTGGCGATTTTGATGCCCTGCGTGGAGAGGTTAAGATTCTTAAGATCGAACCTTTCAATTCTGTCAGGAAGAAAATGTATGTGCTTGTAGCTTATCCTCATGGTGGAACACGAGCTTTTTGCAAAGGTGCATCAGAAATAGTACTGGGAATATGTAACAAGTACATCGACTCTACTGGGGAATCTGTTCATCTCTCCAAAGAAATGGTAAAGAATATCACGGATGTCATAAATTCTTTTGCCTGTGAAGCATTGAGAACTCTCTGCTTAGCGTTTAAGGATATAGATGACTCTTCCATCGTAAATGGCATCCCAGATGATGGCTATACATTGGTAGCAGTTGTCGGTATTAAGGATCCTGTGCGCCCGGGAGTCAGGGAGGCAGTTCAGACTTGTTTAGCTGCTGGAATCACTGTACGTATGGTCACTGGTGACAATATAAATACAGCTAAAGCCATTGCTAAAGAATGTGGCATACTCACGGGGGGTGGTATAGCAATTGAAGGACCTGAGTTTCGTAGCATGTCTCTGGAGCGGATGAAAGCTGTGATACCGAAGATTCAGGTAATGGCCCGGTCTTTACCGTTGGATAAGCACACATTGGTAAAAACTTTGAGAGATGAATTTGGTGAGGTTGTTGCAGTGACTGGTGATGGGACTAACGACGCTCCCGCTTTGCATGAGTCAGACATTGGACTTGCTATGGGCATAGCAGGAACAGAGGTTTGTTGCCAAAGAAAATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

834

Amino Acids

90.63

Weight (kDa)

6.93

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 167 - 234 2.3e-14 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 287 - 383 1.1e-22 P-type ATPase actuator domain
Cation_ATPase PF13246 556 - 708 1.4e-29 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 687 - 813 7.7e-12 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1174
Acc36I ACCTGC 3 cut(s) 422, 1174, 1639
AccB7I CCANNNNNTGG 4 cut(s) 835, 962, 1557, 2075
AccBSI CCGCTC 1 cut(s) 2290
AccII CGCG 2 cut(s) 69, 216
AccIII TCCGGA 1 cut(s) 1064
AciI CCGC 4 cut(s) 795, 1355, 2290, 2427
AclWI GGATC 3 cut(s) 382, 2105, 2118
AcsI RAATTY 4 cut(s) 510, 759, 1987, 2378
AcuI CTGAAG 2 cut(s) 744, 1062
AcvI CACGTG 1 cut(s) 473
AcyI GRCGYC 1 cut(s) 1323
AfaI GTAC 7 cut(s) 72, 78, 746, 983, 1899, 1919, 2172
AfeI AGCGCT 1 cut(s) 272
AfiI CCNNNNNNNGG 8 cut(s) 610, 835, 962, 1183, 1557, 1648, 1859, 2075
AflII CTTAAG 1 cut(s) 1796
AflIII ACRYGT 3 cut(s) 73, 125, 470
AgsI TTSAA 9 cut(s) 149, 299, 335, 383, 702, 1317, 1339, 1813, 2258
AjnI CCWGG 2 cut(s) 303, 828
Alw26I GTCTC 4 cut(s) 544, 1166, 1472, 2286
AlwI GGATC 3 cut(s) 382, 2105, 2118
AlwNI CAGNNNCTG 1 cut(s) 2402
Ama87I CYCGRG 1 cut(s) 2122
Aor13HI TCCGGA 1 cut(s) 1064
Aor51HI AGCGCT 1 cut(s) 272
AoxI GGCC 4 cut(s) 279, 489, 731, 2325
ApeKI GCWGC 4 cut(s) 48, 51, 341, 2156
ApoI RAATTY 4 cut(s) 510, 759, 1987, 2378
ArsI GACNNNNNNTTYG 4 cut(s) 1690, 1722, 2253, 2285
AseI ATTAAT 1 cut(s) 671
Asp700I GAANNNNTTC 3 cut(s) 153, 312, 1941
AspLEI GCGC 4 cut(s) 69, 218, 273, 2121
AspS9I GGNCC 4 cut(s) 732, 946, 2261, 2326
AsuC2I CCSGG 3 cut(s) 2123, 2124, 2329
AsuHPI GGTGA 9 cut(s) 377, 437, 1028, 1042, 1198, 1288, 2198, 2396, 2417
AsuNHI GCTAGC 1 cut(s) 1099
AvaI CYCGRG 1 cut(s) 2122
AvaII GGWCC 2 cut(s) 946, 2261
BaeGI GKGCMC 2 cut(s) 478, 1453
BamHI GGATCC 1 cut(s) 2110
BarI GAAGNNNNNNTAC 2 cut(s) 69, 101
BauI CACGAG 1 cut(s) 1866
BbrPI CACGTG 1 cut(s) 473
BbsI GAAGAC 1 cut(s) 1305
BbvI GCAGC 4 cut(s) 60, 63, 353, 2143
BceAI ACGGC 2 cut(s) 195, 1310
BcgI CGANNNNNNTGC 4 cut(s) 1751, 1785, 1857, 1891
BciT130I CCWGG 2 cut(s) 305, 830
BcnI CCSGG 3 cut(s) 2123, 2124, 2329
BcoDI GTCTC 4 cut(s) 544, 1166, 1472, 2286
BfaI CTAG 5 cut(s) 389, 779, 873, 1100, 1494
BfmI CTRYAG 5 cut(s) 656, 1107, 1181, 1206, 1575
BfoI RGCGCY 1 cut(s) 274
BfrI CTTAAG 1 cut(s) 1796
BfuAI ACCTGC 3 cut(s) 422, 1174, 1639
BglI GCCNNNNNGGC 1 cut(s) 428
BisI GCNGC 4 cut(s) 49, 52, 342, 2157
BlpI GCTNAGC 2 cut(s) 1409, 2023
BlsI GCNGC 4 cut(s) 50, 53, 343, 2158
BmcAI AGTACT 1 cut(s) 1899
Bme1390I CCNGG 5 cut(s) 305, 830, 2123, 2124, 2329
Bme18I GGWCC 2 cut(s) 946, 2261
BmeT110I CYCGRG 1 cut(s) 2122
BmgT120I GGNCC 4 cut(s) 732, 946, 2261, 2326
BmiI GGNNCC 2 cut(s) 947, 2112
BmrFI CCNGG 5 cut(s) 305, 830, 2123, 2124, 2329
BmrI ACTGGG 2 cut(s) 1910, 1941
BmsI GCATC 9 cut(s) 349, 409, 470, 1471, 1640, 1647, 1759, 1895, 2073
BmtI GCTAGC 1 cut(s) 1103
BmuI ACTGGG 2 cut(s) 1910, 1941
BpiI GAAGAC 1 cut(s) 1305
BpmI CTGGAG 2 cut(s) 1647, 2306
Bpu1102I GCTNAGC 2 cut(s) 1409, 2023
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 3 cut(s) 2123, 2124, 2329
Bsa29I ATCGAT 1 cut(s) 260
BsaAI YACGTR 3 cut(s) 473, 744, 2174
BsaHI GRCGYC 1 cut(s) 1323
BsaI GGTCTC 1 cut(s) 1166
BsaJI CCNNGG 4 cut(s) 276, 492, 502, 2122
BsaWI WCCGGW 1 cut(s) 1064
BsaXI ACNNNNNCTCC 2 cut(s) 912, 942
Bsc4I CCNNNNNNNGG 8 cut(s) 610, 835, 962, 1183, 1557, 1648, 1859, 2075
Bse118I RCCGGY 1 cut(s) 1027
Bse1I ACTGG 6 cut(s) 1313, 1522, 1905, 1936, 2188, 2407
Bse3DI GCAATG 1 cut(s) 2211
BseAI TCCGGA 1 cut(s) 1064
BseBI CCWGG 2 cut(s) 305, 830
BseCI ATCGAT 1 cut(s) 260
BseDI CCNNGG 4 cut(s) 276, 492, 502, 2122
BseGI GGATG 8 cut(s) 403, 883, 1087, 1638, 1693, 1984, 2064, 2298
BseLI CCNNNNNNNGG 8 cut(s) 610, 835, 962, 1183, 1557, 1648, 1859, 2075
BseMI GCAATG 1 cut(s) 2211
BseMII CTCAG 5 cut(s) 1142, 1400, 1482, 1665, 2256
BseNI ACTGG 6 cut(s) 1313, 1522, 1905, 1936, 2188, 2407
BseRI GAGGAG 2 cut(s) 198, 1176
BseSI GKGCMC 2 cut(s) 478, 1453
BseXI GCAGC 4 cut(s) 60, 63, 353, 2143
BsgI GTGCAG 1 cut(s) 1097
Bsh1236I CGCG 2 cut(s) 69, 216
Bsh1285I CGRYCG 2 cut(s) 260, 264
BshFI GGCC 4 cut(s) 281, 491, 733, 2327
BshVI ATCGAT 1 cut(s) 260
BsiEI CGRYCG 2 cut(s) 260, 264
BsiHKCI CYCGRG 1 cut(s) 2122
BsiSI CCGG 4 cut(s) 1028, 1065, 2123, 2329
BsiWI CGTACG 1 cut(s) 744
BslFI GGGAC 2 cut(s) 959, 2425
BslI CCNNNNNNNGG 8 cut(s) 610, 835, 962, 1183, 1557, 1648, 1859, 2075
BsmAI GTCTC 4 cut(s) 544, 1166, 1472, 2286
BsmFI GGGAC 2 cut(s) 959, 2425
BsmI GAATGC 1 cut(s) 441
BsnI GGCC 4 cut(s) 281, 491, 733, 2327
Bso31I GGTCTC 1 cut(s) 1166
BsoBI CYCGRG 1 cut(s) 2122
Bsp1286I GDGCHC 2 cut(s) 478, 1453
Bsp13I TCCGGA 1 cut(s) 1064
Bsp143I GATC 7 cut(s) 257, 261, 367, 374, 1299, 1800, 2110
Bsp1720I GCTNAGC 2 cut(s) 1409, 2023
Bsp19I CCATGG 1 cut(s) 502
BspACI CCGC 4 cut(s) 795, 1355, 2290, 2427
BspANI GGCC 4 cut(s) 281, 491, 733, 2327
BspCNI CTCAG 5 cut(s) 1143, 1401, 1481, 1666, 2257
BspDI ATCGAT 1 cut(s) 260
BspEI TCCGGA 1 cut(s) 1064
BspFNI CGCG 2 cut(s) 69, 216
BspLI GGNNCC 2 cut(s) 947, 2112
BspMAI CTGCAG 1 cut(s) 1185
BspMI ACCTGC 3 cut(s) 422, 1174, 1639
BspOI GCTAGC 1 cut(s) 1103
BspPI GGATC 3 cut(s) 382, 2105, 2118
BspTI CTTAAG 1 cut(s) 1796
BspTNI GGTCTC 1 cut(s) 1166
BsrBI CCGCTC 1 cut(s) 2290
BsrDI GCAATG 1 cut(s) 2211
BsrFI RCCGGY 1 cut(s) 1027
BsrI ACTGG 6 cut(s) 1313, 1522, 1905, 1936, 2188, 2407
BssAI RCCGGY 1 cut(s) 1027
BssECI CCNNGG 4 cut(s) 276, 492, 502, 2122
BssMI GATC 7 cut(s) 257, 261, 367, 374, 1299, 1800, 2110
BssNI GRCGYC 1 cut(s) 1323
BssSI CACGAG 1 cut(s) 1866
BssT1I CCWWGG 2 cut(s) 492, 502
Bst2BI CACGAG 1 cut(s) 1866
Bst2UI CCWGG 2 cut(s) 305, 830
Bst4CI ACNGT 8 cut(s) 605, 986, 1111, 1210, 1264, 1598, 2170, 2340
Bst6I CTCTTC 3 cut(s) 108, 1038, 2053
BstACI GRCGYC 1 cut(s) 1323
BstAFI CTTAAG 1 cut(s) 1796
BstAPI GCANNNNNTGC 1 cut(s) 820
BstBAI YACGTR 3 cut(s) 473, 744, 2174
BstC8I GCNNGC 2 cut(s) 731, 1101
BstDEI CTNAG 6 cut(s) 1151, 1409, 1468, 1674, 2023, 2265
BstDSI CCRYGG 1 cut(s) 502
BstEII GGTNACC 1 cut(s) 790
BstENI CCTNNNNNAGG 1 cut(s) 1646
BstF5I GGATG 8 cut(s) 403, 883, 1087, 1638, 1693, 1984, 2064, 2298
BstFNI CGCG 2 cut(s) 69, 216
BstH2I RGCGCY 1 cut(s) 274
BstHHI GCGC 4 cut(s) 69, 218, 273, 2121
BstKTI GATC 7 cut(s) 260, 264, 370, 377, 1302, 1803, 2113
BstMAI GTCTC 4 cut(s) 544, 1166, 1472, 2286
BstMBI GATC 7 cut(s) 257, 261, 367, 374, 1299, 1800, 2110
BstMCI CGRYCG 2 cut(s) 260, 264
BstMWI GCNNNNNNNGC 4 cut(s) 428, 820, 1189, 2003
BstNI CCWGG 2 cut(s) 305, 830
BstNSI RCATGY 3 cut(s) 77, 1476, 2281
BstPI GGTNACC 1 cut(s) 790
BstSCI CCNGG 5 cut(s) 303, 828, 2121, 2122, 2327
BstSFI CTRYAG 5 cut(s) 656, 1107, 1181, 1206, 1575
BstSLI GKGCMC 2 cut(s) 478, 1453
BstSNI TACGTA 1 cut(s) 2174
BstUI CGCG 2 cut(s) 69, 216
BstV1I GCAGC 4 cut(s) 60, 63, 353, 2143
BstV2I GAAGAC 1 cut(s) 1305
BstX2I RGATCY 2 cut(s) 374, 2110
BstXI CCANNNNNNTGG 1 cut(s) 1960
BstYI RGATCY 2 cut(s) 374, 2110
Bsu15I ATCGAT 1 cut(s) 260
BsuRI GGCC 4 cut(s) 281, 491, 733, 2327
BsuTUI ATCGAT 1 cut(s) 260
BtgI CCRYGG 1 cut(s) 502
BtsCI GGATG 8 cut(s) 403, 883, 1087, 1638, 1693, 1984, 2064, 2298
BtsI GCAGTG 5 cut(s) 344, 1178, 1245, 1407, 2403
BtsIMutI CAGTG 9 cut(s) 344, 720, 1178, 1245, 1302, 1407, 2166, 2181, 2403
BveI ACCTGC 3 cut(s) 422, 1174, 1639
Cac8I GCNNGC 2 cut(s) 731, 1101
CaiI CAGNNNCTG 1 cut(s) 2402
CfoI GCGC 4 cut(s) 69, 218, 273, 2121
Cfr10I RCCGGY 1 cut(s) 1027
Cfr13I GGNCC 4 cut(s) 732, 946, 2261, 2326
Cfr9I CCCGGG 1 cut(s) 2122
ClaI ATCGAT 1 cut(s) 260
CseI GACGC 3 cut(s) 1331, 1414, 2429
Csp6I GTAC 7 cut(s) 71, 77, 745, 982, 1898, 1918, 2171
CviAII CATG 8 cut(s) 74, 479, 503, 515, 1473, 1856, 2278, 2435
CviQI GTAC 7 cut(s) 71, 77, 745, 982, 1898, 1918, 2171
DdeI CTNAG 6 cut(s) 1151, 1409, 1468, 1674, 2023, 2265
DpnI GATC 7 cut(s) 259, 263, 369, 376, 1301, 1802, 2112
DpnII GATC 7 cut(s) 257, 261, 367, 374, 1299, 1800, 2110
Eam1104I CTCTTC 3 cut(s) 108, 1038, 2053
EarI CTCTTC 3 cut(s) 108, 1038, 2053
Eco105I TACGTA 1 cut(s) 2174
Eco130I CCWWGG 2 cut(s) 492, 502
Eco147I AGGCCT 1 cut(s) 491
Eco31I GGTCTC 1 cut(s) 1166
Eco32I GATATC 1 cut(s) 1614
Eco47I GGWCC 2 cut(s) 946, 2261
Eco47III AGCGCT 1 cut(s) 272
Eco57I CTGAAG 2 cut(s) 744, 1062
Eco72I CACGTG 1 cut(s) 473
Eco88I CYCGRG 1 cut(s) 2122
Eco91I GGTNACC 1 cut(s) 790
EcoNI CCTNNNNNAGG 1 cut(s) 1646
EcoO109I RGGNCCY 1 cut(s) 2261
EcoO65I GGTNACC 1 cut(s) 790
EcoRI GAATTC 1 cut(s) 510
EcoRII CCWGG 2 cut(s) 303, 828
EcoRV GATATC 1 cut(s) 1614
EcoT14I CCWWGG 2 cut(s) 492, 502
ErhI CCWWGG 2 cut(s) 492, 502
FaeI CATG 8 cut(s) 77, 482, 506, 518, 1476, 1859, 2281, 2438
FaqI GGGAC 2 cut(s) 959, 2425
FatI CATG 8 cut(s) 73, 478, 502, 514, 1472, 1855, 2277, 2434
FauI CCCGC 1 cut(s) 2434
FauNDI CATATG 1 cut(s) 1539
Fnu4HI GCNGC 4 cut(s) 49, 52, 342, 2157
FokI GGATG 8 cut(s) 410, 890, 1094, 1625, 1700, 1991, 2051, 2305
Fsp4HI GCNGC 4 cut(s) 49, 52, 342, 2157
FspBI CTAG 5 cut(s) 389, 779, 873, 1100, 1494
GlaI GCGC 4 cut(s) 68, 217, 272, 2120
GluI GCNGC 4 cut(s) 49, 52, 342, 2157
GsuI CTGGAG 2 cut(s) 1647, 2306
HaeII RGCGCY 1 cut(s) 274
HaeIII GGCC 4 cut(s) 281, 491, 733, 2327
HapII CCGG 4 cut(s) 1028, 1065, 2123, 2329
HgaI GACGC 3 cut(s) 1331, 1414, 2429
HhaI GCGC 4 cut(s) 69, 218, 273, 2121
Hin1I GRCGYC 1 cut(s) 1323
Hin1II CATG 8 cut(s) 77, 482, 506, 518, 1476, 1859, 2281, 2438
Hin6I GCGC 4 cut(s) 67, 216, 271, 2119
HinP1I GCGC 4 cut(s) 67, 216, 271, 2119
HincII GTYRAC 1 cut(s) 1245
HindII GTYRAC 1 cut(s) 1245
HindIII AAGCTT 3 cut(s) 293, 1006, 1190
HpaII CCGG 4 cut(s) 1028, 1065, 2123, 2329
HphI GGTGA 9 cut(s) 377, 437, 1028, 1042, 1198, 1288, 2198, 2396, 2417
Hpy166II GTNNAC 2 cut(s) 1245, 1748
Hpy188III TCNNGA 7 cut(s) 146, 395, 1065, 1382, 1626, 1822, 2285
Hpy8I GTNNAC 2 cut(s) 1245, 1748
Hpy99I CGWCG 4 cut(s) 93, 216, 255, 2423
HpyAV CCTTC 7 cut(s) 143, 232, 545, 719, 1151, 1379, 2252
HpyCH4III ACNGT 8 cut(s) 605, 986, 1111, 1210, 1264, 1598, 2170, 2340
HpyCH4IV ACGT 4 cut(s) 125, 472, 743, 2173
HpyF10VI GCNNNNNNNGC 4 cut(s) 428, 820, 1189, 2003
HpyF3I CTNAG 6 cut(s) 1151, 1409, 1468, 1674, 2023, 2265
HpySE526I ACGT 4 cut(s) 125, 472, 743, 2173
Hsp92I GRCGYC 1 cut(s) 1323
Hsp92II CATG 8 cut(s) 77, 482, 506, 518, 1476, 1859, 2281, 2438
HspAI GCGC 4 cut(s) 67, 216, 271, 2119
Kpn2I TCCGGA 1 cut(s) 1064
Kzo9I GATC 7 cut(s) 257, 261, 367, 374, 1299, 1800, 2110
LmnI GCTCC 4 cut(s) 278, 1628, 2287, 2428
Lsp1109I GCAGC 4 cut(s) 60, 63, 353, 2143
LweI GCATC 9 cut(s) 349, 409, 470, 1471, 1640, 1647, 1759, 1895, 2073
MaeI CTAG 5 cut(s) 389, 779, 873, 1100, 1494
MaeII ACGT 4 cut(s) 125, 472, 743, 2173
MalI GATC 7 cut(s) 259, 263, 369, 376, 1301, 1802, 2112
MbiI CCGCTC 1 cut(s) 2290
MboI GATC 7 cut(s) 257, 261, 367, 374, 1299, 1800, 2110
MfeI CAATTG 2 cut(s) 702, 2253
MflI RGATCY 2 cut(s) 374, 2110
MhlI GDGCHC 2 cut(s) 478, 1453
MlyI GAGTC 6 cut(s) 1010, 1733, 1919, 2039, 2136, 2447
MmeI TCCRAC 3 cut(s) 691, 898, 2322
MroI TCCGGA 1 cut(s) 1064
MroXI GAANNNNTTC 3 cut(s) 153, 312, 1941
MslI CAYNNNNRTG 1 cut(s) 450
MspA1I CMGCKG 1 cut(s) 959
MspCI CTTAAG 1 cut(s) 1796
MspI CCGG 4 cut(s) 1028, 1065, 2123, 2329
MspR9I CCNGG 5 cut(s) 305, 830, 2123, 2124, 2329
MunI CAATTG 2 cut(s) 702, 2253
Mva1269I GAATGC 1 cut(s) 441
MvaI CCWGG 2 cut(s) 305, 830
MvnI CGCG 2 cut(s) 69, 216
MwoI GCNNNNNNNGC 4 cut(s) 428, 820, 1189, 2003
NciI CCSGG 3 cut(s) 2123, 2124, 2329
NcoI CCATGG 1 cut(s) 502
NdeI CATATG 1 cut(s) 1539
NdeII GATC 7 cut(s) 257, 261, 367, 374, 1299, 1800, 2110
NheI GCTAGC 1 cut(s) 1099
NlaIII CATG 8 cut(s) 77, 482, 506, 518, 1476, 1859, 2281, 2438
NlaIV GGNNCC 2 cut(s) 947, 2112
NmuCI GTSAC 7 cut(s) 803, 868, 1276, 1402, 2179, 2186, 2398
NspI RCATGY 3 cut(s) 77, 1476, 2281
PaqCI CACCTGC 1 cut(s) 1174
PceI AGGCCT 1 cut(s) 491
PciI ACATGT 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 85
PctI GAATGC 1 cut(s) 441
PdmI GAANNNNTTC 3 cut(s) 153, 312, 1941
PfeI GAWTC 8 cut(s) 82, 173, 247, 313, 1792, 1937, 2163, 2314
Pfl23II CGTACG 1 cut(s) 744
PflMI CCANNNNNTGG 4 cut(s) 835, 962, 1557, 2075
PfoI TCCNGGA 2 cut(s) 303, 828
PkrI GCNGC 4 cut(s) 50, 53, 343, 2158
Ple19I CGATCG 2 cut(s) 260, 264
PleI GAGTC 6 cut(s) 1009, 1732, 1919, 2039, 2135, 2446
PmaCI CACGTG 1 cut(s) 473
PmlI CACGTG 1 cut(s) 473
PpsI GAGTC 6 cut(s) 1009, 1732, 1919, 2039, 2135, 2446
Ppu21I YACGTR 3 cut(s) 473, 744, 2174
PpuMI RGGWCCY 1 cut(s) 2261
PscI ACATGT 1 cut(s) 73
PshBI ATTAAT 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 2261
Psp6I CCWGG 2 cut(s) 303, 828
PspCI CACGTG 1 cut(s) 473
PspEI GGTNACC 1 cut(s) 790
PspGI CCWGG 2 cut(s) 303, 828
PspLI CGTACG 1 cut(s) 744
PspN4I GGNNCC 2 cut(s) 947, 2112
PspPI GGNCC 4 cut(s) 732, 946, 2261, 2326
PspPPI RGGWCCY 1 cut(s) 2261
PsrI GAACNNNNNNTAC 2 cut(s) 1513, 1545
PstI CTGCAG 1 cut(s) 1185
PstNI CAGNNNCTG 1 cut(s) 2402
PsuI RGATCY 2 cut(s) 374, 2110
PvuI CGATCG 2 cut(s) 260, 264
PvuII CAGCTG 1 cut(s) 959
RsaI GTAC 7 cut(s) 72, 78, 746, 983, 1899, 1919, 2172
RsaNI GTAC 7 cut(s) 71, 77, 745, 982, 1898, 1918, 2171
RseI CAYNNNNRTG 1 cut(s) 450
SatI GCNGC 4 cut(s) 49, 52, 342, 2157
Sau3AI GATC 7 cut(s) 257, 261, 367, 374, 1299, 1800, 2110
Sau96I GGNCC 4 cut(s) 732, 946, 2261, 2326
ScaI AGTACT 1 cut(s) 1899
SchI GAGTC 6 cut(s) 1010, 1733, 1919, 2039, 2136, 2447
ScrFI CCNGG 5 cut(s) 305, 830, 2123, 2124, 2329
SduI GDGCHC 2 cut(s) 478, 1453
SfaNI GCATC 9 cut(s) 349, 409, 470, 1471, 1640, 1647, 1759, 1895, 2073
SfcI CTRYAG 5 cut(s) 656, 1107, 1181, 1206, 1575
SinI GGWCC 2 cut(s) 946, 2261
SmaI CCCGGG 1 cut(s) 2124
SmiMI CAYNNNNRTG 1 cut(s) 450
SmlI CTYRAG 2 cut(s) 404, 1796
SmoI CTYRAG 2 cut(s) 404, 1796
SnaBI TACGTA 1 cut(s) 2174
SseBI AGGCCT 1 cut(s) 491
SsiI CCGC 4 cut(s) 795, 1355, 2290, 2427
SspMI CTAG 5 cut(s) 389, 779, 873, 1100, 1494
StuI AGGCCT 1 cut(s) 491
StyD4I CCNGG 5 cut(s) 303, 828, 2121, 2122, 2327
StyI CCWWGG 2 cut(s) 492, 502
TaaI ACNGT 8 cut(s) 605, 986, 1111, 1210, 1264, 1598, 2170, 2340
TaiI ACGT 4 cut(s) 128, 475, 746, 2176
TaqI TCGA 7 cut(s) 157, 245, 260, 316, 938, 1803, 1923
TatI WGTACW 2 cut(s) 1897, 1917
TfiI GAWTC 8 cut(s) 82, 173, 247, 313, 1792, 1937, 2163, 2314
TscAI CASTG 9 cut(s) 351, 727, 1185, 1245, 1309, 1407, 2173, 2188, 2403
TseFI GTSAC 7 cut(s) 803, 868, 1276, 1402, 2179, 2186, 2398
TseI GCWGC 4 cut(s) 48, 51, 341, 2156
Tsp45I GTSAC 7 cut(s) 803, 868, 1276, 1402, 2179, 2186, 2398
TspGWI ACGGA 3 cut(s) 84, 94, 1991
TspMI CCCGGG 1 cut(s) 2122
TspRI CASTG 9 cut(s) 351, 727, 1185, 1245, 1309, 1407, 2173, 2188, 2403
Van91I CCANNNNNTGG 4 cut(s) 835, 962, 1557, 2075
Vha464I CTTAAG 1 cut(s) 1796
VpaK11BI GGWCC 2 cut(s) 946, 2261
VspI ATTAAT 1 cut(s) 671
XagI CCTNNNNNAGG 1 cut(s) 1646
XapI RAATTY 4 cut(s) 510, 759, 1987, 2378
XceI RCATGY 3 cut(s) 77, 1476, 2281
XcmI CCANNNNNNNNNTGG 1 cut(s) 907
XmaI CCCGGG 1 cut(s) 2122
XmnI GAANNNNTTC 3 cut(s) 153, 312, 1941
XspI CTAG 5 cut(s) 389, 779, 873, 1100, 1494
ZrmI AGTACT 1 cut(s) 1899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.