pycom12g06980

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0006634
Physical Location & Seq
Forward (+)
7263444 .. 7265928
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1002 bp
ATGTCAGGGTACCCACACCCTCCTCCCGGCTACGGCTACGGCAGCGCTCCACCACCAGGCCAACCTTACGGCGCGCCGCCCCAAGGCCAACCCTACGGCGCGCCGCCCCAAGGCCAACCCTACGGCGCACCGCCGCAAGGGCAGCACCAGCACCAGACAGCCCAACCCTACGGTGCTCCGTCTGCACCGTACGGGGCCCCATCCTCCGCACCGTACGGTTCTCCGGCAGCACCGTACGGCGCGGCCTACGCGCCAGAGAAGCCGTACAATCCAGATAAGCCGTATAATCCCGACAAGCCTCACAAGAATCAAGGTGGCGGAGGCGGAGGAGGTGGCGGAGGCGGATACCCGAATCAAGGTGGAGGAGGCGGAGGCGGATACCCGAATCAAGGTGGAGGAGGAGGAGGCGGAGGCGGATACCCGCCACCTGCTCATGGTTACGGGAGCCCGTTTGCGGCTTTATTGCCATCGGTGTTCCCGCCGGGCACAGACCCGAACGTCATAGCTTGCTTTCAGCTGGCGGATCAGGACGGCAGCGGCTTCATCGACGACAAGGAGATGCAGAGAGCTCTGTCGTCGTACAATGAGTGCTTCTGCTTGAGGACTGTTCATCTTCTCATGTACACCTTCACCCAGACCAACGCCAGGAAGATCGGGCCCAAGGAATTCGCTGCACTGTTCTACAGTCTTCAAAGCTGGAGGGGAATATTTGAGAGGTCGGACCGGGACAGAAGTGGGTTCATTGATTCAAATGAGTTGAGAGATGCACTGCTGAGTCTGGGATTTTCTGTGTCACCTGTAGTTTTGGAACTGCTGGTCTCTAAATTCGACAAGTCCGGAGGCAAAAAGAGGGCCATTGAATATGACAATTTCATCGAGTGCTGTCTGACTGTTAAGGGATTAACTGAGAAGTTTAAGGAGAAGGACAAAGCGTACACTGGTATGGGAACTTTCAGTTATGAGGAGTTCATGTTGACCGTCCTGCCATTCCTCATTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

334

Amino Acids

35.04

Weight (kDa)

6.3

Isoelectric Point (pI)

56.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 233 - 293 1.4e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 436
AasI GACNNNNNNGTC 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 436
Acc65I GGTACC 1 cut(s) 9
AccB1I GGYRCC 1 cut(s) 9
AccII CGCG 4 cut(s) 74, 101, 242, 251
AccIII TCCGGA 1 cut(s) 836
AclWI GGATC 1 cut(s) 531
AcsI RAATTY 2 cut(s) 665, 824
AfaI GTAC 8 cut(s) 11, 191, 215, 236, 266, 581, 623, 935
AfeI AGCGCT 1 cut(s) 46
AgsI TTSAA 3 cut(s) 692, 750, 860
AjnI CCWGG 2 cut(s) 55, 644
AluBI AGCT 4 cut(s) 506, 517, 569, 696
AluI AGCT 4 cut(s) 506, 517, 569, 696
Alw21I GWGCWC 2 cut(s) 178, 571
Alw26I GTCTC 1 cut(s) 823
AlwI GGATC 1 cut(s) 531
Aor13HI TCCGGA 1 cut(s) 836
Aor51HI AGCGCT 1 cut(s) 46
AoxI GGCC 7 cut(s) 58, 85, 112, 195, 243, 656, 852
ApaI GGGCCC 2 cut(s) 199, 660
ApeKI GCWGC 5 cut(s) 42, 142, 227, 534, 671
ApoI RAATTY 2 cut(s) 665, 824
AscI GGCGCGCC 2 cut(s) 72, 99
Asp718I GGTACC 1 cut(s) 9
AspLEI GCGC 8 cut(s) 47, 74, 76, 101, 103, 128, 242, 253
AspS9I GGNCC 6 cut(s) 195, 196, 656, 657, 721, 852
AsuC2I CCSGG 3 cut(s) 27, 483, 725
AsuHPI GGTGA 2 cut(s) 622, 786
AvaII GGWCC 1 cut(s) 721
BaeGI GKGCMC 3 cut(s) 199, 488, 660
BanI GGYRCC 1 cut(s) 9
BanII GRGCYC 4 cut(s) 199, 449, 571, 660
BbsI GAAGAC 1 cut(s) 680
Bbv12I GWGCWC 2 cut(s) 178, 571
BbvI GCAGC 5 cut(s) 54, 154, 239, 546, 658
BccI CCATC 2 cut(s) 208, 475
BceAI ACGGC 9 cut(s) 49, 55, 85, 112, 139, 247, 253, 265, 547
BciT130I CCWGG 2 cut(s) 57, 646
BciVI GTATCC 3 cut(s) 338, 371, 410
BcnI CCSGG 3 cut(s) 27, 483, 725
BcoDI GTCTC 1 cut(s) 823
BfmI CTRYAG 2 cut(s) 682, 798
BfoI RGCGCY 1 cut(s) 48
BfuAI ACCTGC 1 cut(s) 436
BfuI GTATCC 3 cut(s) 338, 371, 410
BglI GCCNNNNNGGC 1 cut(s) 139
Bme1390I CCNGG 5 cut(s) 27, 57, 483, 646, 725
Bme18I GGWCC 1 cut(s) 721
BmgT120I GGNCC 6 cut(s) 195, 196, 656, 657, 721, 852
BmiI GGNNCC 6 cut(s) 11, 196, 197, 198, 446, 658
BmrFI CCNGG 5 cut(s) 27, 57, 483, 646, 725
BmsI GCATC 2 cut(s) 549, 754
BpiI GAAGAC 1 cut(s) 680
BpmI CTGGAG 1 cut(s) 718
BpuEI CTTGAG 1 cut(s) 619
BpuMI CCSGG 3 cut(s) 27, 483, 725
BsaI GGTCTC 1 cut(s) 823
BsaJI CCNNGG 3 cut(s) 82, 109, 660
BsaWI WCCGGW 1 cut(s) 836
BsaXI ACNNNNNCTCC 2 cut(s) 956, 986
Bse1I ACTGG 1 cut(s) 943
Bse3DI GCAATG 1 cut(s) 993
BseAI TCCGGA 1 cut(s) 836
BseBI CCWGG 2 cut(s) 57, 646
BseDI CCNNGG 3 cut(s) 82, 109, 660
BseGI GGATG 1 cut(s) 200
BseMI GCAATG 1 cut(s) 993
BseMII CTCAG 2 cut(s) 764, 897
BseNI ACTGG 1 cut(s) 943
BsePI GCGCGC 2 cut(s) 72, 99
BseRI GAGGAG 7 cut(s) 12, 342, 378, 411, 414, 417, 977
BseSI GKGCMC 3 cut(s) 199, 488, 660
BseXI GCAGC 5 cut(s) 54, 154, 239, 546, 658
BsgI GTGCAG 2 cut(s) 168, 657
Bsh1236I CGCG 4 cut(s) 74, 101, 242, 251
BshFI GGCC 7 cut(s) 60, 87, 114, 197, 245, 658, 854
BshNI GGYRCC 1 cut(s) 9
BsiHKAI GWGCWC 2 cut(s) 178, 571
BsiSI CCGG 5 cut(s) 27, 224, 482, 724, 837
BsiWI CGTACG 3 cut(s) 189, 213, 234
BslFI GGGAC 1 cut(s) 740
BsmAI GTCTC 1 cut(s) 823
BsmFI GGGAC 1 cut(s) 740
BsnI GGCC 7 cut(s) 60, 87, 114, 197, 245, 658, 854
Bso31I GGTCTC 1 cut(s) 823
Bsp120I GGGCCC 2 cut(s) 195, 656
Bsp1286I GDGCHC 6 cut(s) 178, 199, 449, 488, 571, 660
Bsp13I TCCGGA 1 cut(s) 836
Bsp1407I TGTACA 1 cut(s) 621
Bsp143I GATC 2 cut(s) 523, 651
BspANI GGCC 7 cut(s) 60, 87, 114, 197, 245, 658, 854
BspCNI CTCAG 2 cut(s) 765, 898
BspEI TCCGGA 1 cut(s) 836
BspFNI CGCG 4 cut(s) 74, 101, 242, 251
BspLI GGNNCC 6 cut(s) 11, 196, 197, 198, 446, 658
BspMI ACCTGC 1 cut(s) 436
BspPI GGATC 1 cut(s) 531
BspT107I GGYRCC 1 cut(s) 9
BspTNI GGTCTC 1 cut(s) 823
BsrDI GCAATG 1 cut(s) 993
BsrGI TGTACA 1 cut(s) 621
BsrI ACTGG 1 cut(s) 943
BssECI CCNNGG 3 cut(s) 82, 109, 660
BssHII GCGCGC 2 cut(s) 72, 99
BssMI GATC 2 cut(s) 523, 651
BssT1I CCWWGG 3 cut(s) 82, 109, 660
Bst2UI CCWGG 2 cut(s) 57, 646
BstAUI TGTACA 1 cut(s) 621
BstC8I GCNNGC 4 cut(s) 74, 101, 508, 519
BstDEI CTNAG 2 cut(s) 773, 906
BstF5I GGATG 1 cut(s) 200
BstFNI CGCG 4 cut(s) 74, 101, 242, 251
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 8 cut(s) 47, 74, 76, 101, 103, 128, 242, 253
BstKTI GATC 2 cut(s) 526, 654
BstMAI GTCTC 1 cut(s) 823
BstMBI GATC 2 cut(s) 523, 651
BstMWI GCNNNNNNNGC 7 cut(s) 42, 139, 142, 148, 182, 248, 259
BstNI CCWGG 2 cut(s) 57, 646
BstSCI CCNGG 5 cut(s) 25, 55, 481, 644, 723
BstSFI CTRYAG 2 cut(s) 682, 798
BstSLI GKGCMC 3 cut(s) 199, 488, 660
BstUI CGCG 4 cut(s) 74, 101, 242, 251
BstV1I GCAGC 5 cut(s) 54, 154, 239, 546, 658
BstV2I GAAGAC 1 cut(s) 680
BsuI GTATCC 3 cut(s) 338, 371, 410
BsuRI GGCC 7 cut(s) 60, 87, 114, 197, 245, 658, 854
BtsCI GGATG 1 cut(s) 200
BtsI GCAGTG 1 cut(s) 767
BtsIMutI CAGTG 3 cut(s) 674, 767, 936
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 4 cut(s) 74, 101, 508, 519
CfoI GCGC 8 cut(s) 47, 74, 76, 101, 103, 128, 242, 253
Cfr13I GGNCC 6 cut(s) 195, 196, 656, 657, 721, 852
CpoI CGGWCCG 1 cut(s) 721
Csp6I GTAC 8 cut(s) 10, 190, 214, 235, 265, 580, 622, 934
CspI CGGWCCG 1 cut(s) 721
CviAII CATG 3 cut(s) 434, 619, 970
CviQI GTAC 8 cut(s) 10, 190, 214, 235, 265, 580, 622, 934
DdeI CTNAG 2 cut(s) 773, 906
DpnI GATC 2 cut(s) 525, 653
DpnII GATC 2 cut(s) 523, 651
DrdI GACNNNNNNGTC 1 cut(s) 497
DseDI GACNNNNNNGTC 1 cut(s) 497
EciI GGCGGA 9 cut(s) 333, 339, 351, 357, 384, 390, 423, 429, 536
Ecl136II GAGCTC 1 cut(s) 569
Eco130I CCWWGG 3 cut(s) 82, 109, 660
Eco24I GRGCYC 4 cut(s) 199, 449, 571, 660
Eco31I GGTCTC 1 cut(s) 823
Eco47I GGWCC 1 cut(s) 721
Eco47III AGCGCT 1 cut(s) 46
Eco53kI GAGCTC 1 cut(s) 569
EcoICRI GAGCTC 1 cut(s) 569
EcoO109I RGGNCCY 2 cut(s) 195, 196
EcoRI GAATTC 1 cut(s) 665
EcoRII CCWGG 2 cut(s) 55, 644
EcoT14I CCWWGG 3 cut(s) 82, 109, 660
EcoT38I GRGCYC 4 cut(s) 199, 449, 571, 660
ErhI CCWWGG 3 cut(s) 82, 109, 660
FaeI CATG 3 cut(s) 437, 622, 973
FaiI YATR 8 cut(s) 285, 435, 503, 620, 864, 944, 960, 971
FaqI GGGAC 1 cut(s) 740
FatI CATG 3 cut(s) 433, 618, 969
FauI CCCGC 2 cut(s) 429, 486
FokI GGATG 1 cut(s) 187
FriOI GRGCYC 4 cut(s) 199, 449, 571, 660
GlaI GCGC 8 cut(s) 46, 73, 75, 100, 102, 127, 241, 252
GsuI CTGGAG 1 cut(s) 718
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 7 cut(s) 60, 87, 114, 197, 245, 658, 854
HapII CCGG 5 cut(s) 27, 224, 482, 724, 837
HhaI GCGC 8 cut(s) 47, 74, 76, 101, 103, 128, 242, 253
Hin1II CATG 3 cut(s) 437, 622, 973
Hin6I GCGC 8 cut(s) 45, 72, 74, 99, 101, 126, 240, 251
HinP1I GCGC 8 cut(s) 45, 72, 74, 99, 101, 126, 240, 251
HincII GTYRAC 1 cut(s) 975
HindII GTYRAC 1 cut(s) 975
HinfI GANTC 5 cut(s) 307, 352, 385, 746, 775
HpaII CCGG 5 cut(s) 27, 224, 482, 724, 837
HphI GGTGA 2 cut(s) 622, 786
Hpy166II GTNNAC 3 cut(s) 624, 936, 975
Hpy188I TCNGA 2 cut(s) 721, 888
Hpy188III TCNNGA 4 cut(s) 272, 290, 527, 837
Hpy8I GTNNAC 3 cut(s) 624, 936, 975
Hpy99I CGWCG 2 cut(s) 551, 580
HpyAV CCTTC 2 cut(s) 637, 916
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 4 cut(s) 185, 562, 674, 767
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 139, 142, 148, 182, 248, 259
HpyF3I CTNAG 2 cut(s) 773, 906
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 3 cut(s) 437, 622, 973
HspAI GCGC 8 cut(s) 45, 72, 74, 99, 101, 126, 240, 251
Kpn2I TCCGGA 1 cut(s) 836
KpnI GGTACC 1 cut(s) 13
Kzo9I GATC 2 cut(s) 523, 651
LmnI GCTCC 3 cut(s) 52, 181, 444
Lsp1109I GCAGC 5 cut(s) 54, 154, 239, 546, 658
LweI GCATC 2 cut(s) 549, 754
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 2 cut(s) 437, 792
MalI GATC 2 cut(s) 525, 653
MboI GATC 2 cut(s) 523, 651
MboII GAAGA 3 cut(s) 605, 661, 680
MhlI GDGCHC 6 cut(s) 178, 199, 449, 488, 571, 660
MluCI AATT 3 cut(s) 665, 824, 868
MlyI GAGTC 1 cut(s) 784
MmeI TCCRAC 1 cut(s) 699
MroI TCCGGA 1 cut(s) 836
MseI TTAA 3 cut(s) 894, 902, 915
MslI CAYNNNNRTG 1 cut(s) 941
MspA1I CMGCKG 2 cut(s) 517, 537
MspI CCGG 5 cut(s) 27, 224, 482, 724, 837
MspR9I CCNGG 5 cut(s) 27, 57, 483, 646, 725
MvaI CCWGG 2 cut(s) 57, 646
MvnI CGCG 4 cut(s) 74, 101, 242, 251
MwoI GCNNNNNNNGC 7 cut(s) 42, 139, 142, 148, 182, 248, 259
NciI CCSGG 3 cut(s) 27, 483, 725
NdeII GATC 2 cut(s) 523, 651
NlaIII CATG 3 cut(s) 437, 622, 973
NlaIV GGNNCC 6 cut(s) 11, 196, 197, 198, 446, 658
NmuCI GTSAC 1 cut(s) 792
PalAI GGCGCGCC 2 cut(s) 72, 99
PaqCI CACCTGC 1 cut(s) 436
PauI GCGCGC 2 cut(s) 72, 99
PfeI GAWTC 4 cut(s) 307, 352, 385, 746
Pfl23II CGTACG 3 cut(s) 189, 213, 234
PleI GAGTC 1 cut(s) 783
PpsI GAGTC 1 cut(s) 783
Psp124BI GAGCTC 1 cut(s) 571
Psp6I CCWGG 2 cut(s) 55, 644
PspGI CCWGG 2 cut(s) 55, 644
PspLI CGTACG 3 cut(s) 189, 213, 234
PspN4I GGNNCC 6 cut(s) 11, 196, 197, 198, 446, 658
PspOMI GGGCCC 2 cut(s) 195, 656
PspPI GGNCC 6 cut(s) 195, 196, 656, 657, 721, 852
PteI GCGCGC 2 cut(s) 72, 99
PvuII CAGCTG 1 cut(s) 517
RsaI GTAC 8 cut(s) 11, 191, 215, 236, 266, 581, 623, 935
RsaNI GTAC 8 cut(s) 10, 190, 214, 235, 265, 580, 622, 934
RseI CAYNNNNRTG 1 cut(s) 941
Rsr2I CGGWCCG 1 cut(s) 721
RsrII CGGWCCG 1 cut(s) 721
SacI GAGCTC 1 cut(s) 571
SaqAI TTAA 3 cut(s) 894, 902, 915
Sau3AI GATC 2 cut(s) 523, 651
Sau96I GGNCC 6 cut(s) 195, 196, 656, 657, 721, 852
SchI GAGTC 1 cut(s) 784
ScrFI CCNGG 5 cut(s) 27, 57, 483, 646, 725
SduI GDGCHC 6 cut(s) 178, 199, 449, 488, 571, 660
SfaNI GCATC 2 cut(s) 549, 754
SfcI CTRYAG 2 cut(s) 682, 798
SgsI GGCGCGCC 2 cut(s) 72, 99
SinI GGWCC 1 cut(s) 721
SmiMI CAYNNNNRTG 1 cut(s) 941
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
Sse9I AATT 3 cut(s) 665, 824, 868
SspI AATATT 1 cut(s) 708
SstI GAGCTC 1 cut(s) 571
StyD4I CCNGG 5 cut(s) 25, 55, 481, 644, 723
StyI CCWWGG 3 cut(s) 82, 109, 660
TaiI ACGT 1 cut(s) 501
TaqI TCGA 3 cut(s) 546, 828, 876
TasI AATT 3 cut(s) 665, 824, 868
TatI WGTACW 1 cut(s) 621
TauI GCSGC 6 cut(s) 79, 106, 136, 245, 458, 540
TfiI GAWTC 4 cut(s) 307, 352, 385, 746
Tru1I TTAA 3 cut(s) 894, 902, 915
Tru9I TTAA 3 cut(s) 894, 902, 915
TscAI CASTG 3 cut(s) 681, 774, 943
TseFI GTSAC 1 cut(s) 792
TseI GCWGC 5 cut(s) 42, 142, 227, 534, 671
Tsp45I GTSAC 1 cut(s) 792
TspDTI ATGAA 5 cut(s) 532, 599, 730, 862, 958
TspGWI ACGGA 1 cut(s) 168
TspRI CASTG 3 cut(s) 681, 774, 943
VpaK11BI GGWCC 1 cut(s) 721
XapI RAATTY 2 cut(s) 665, 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.