Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0006287

Physical Location & Seq

Genomic Coordinates Reverse (-)

21403992 .. 21406185

Gene Structure 2194 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGTTTTCGCGTTATTTGTTTTTTGTAATGCTCTCGAGAGGAGAGAGAGAGAGGAGAAACAGTAGCATTTCGGACCATTTTCCATTGTTTCCGGTGCTCGCTTTCTCTCTCAGGTGGATTTTTGGGTGCGAGGAACAGATGGTAAAGGGAGGGAAGGTTAGTTCCAAGCGAAAAATCAAGGCAAGGATTCCGTCAAAGTATCAGGGTTCCGATGATTCGGATGAGGATTATGTGATTTCGGGGGAAGAAAATGAAGTTTCTGAGGATTTAGAGGAGGATTACTGTTCTTCTTTGGATGGAGATGCGTCAGAAGACAGCTTTGGGAGTTTTGCGGACGAGGAGGAAGAGGAGGAGGAAGTGAAAAAGGTGAGGAAGAACAGTAGATCAAGAGCGAAAAGGGGTTCTGCTGGTTTAATTGTGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGACAAAGAGGAAGTGAAACCGGTTAGGAAGGGAATCAGGTCAAAAGTCCGAAATGGTTTTTCTGGTCGGCAGAAAAATGGGGTTAAACCGTTACGAAAGAGGAGAATGATTGCATATCGTGAGGTGGAGGATCAGGATTATAATGATGAAGATGATGATGACGATGAGGAATTTACACCAGATGAAGATGAATTTTCAGAGGTGGAAGAAAAACAAGTGGTGAAAAAGAAGAAAACTAATGGGAAACTGGGCAAGCGGCGGTTGAAGAAAAAGGGTCATTCTGTAAGAGGTGAAAAAAGGAGAAAATCTATGGCTTTAAAGAAGCCTTTGAGAAACAAAGGTAGAACGAGTCAGGCGATGGGGAGGAAGGCAAGAAGGGATGATGAACTTGAAAGTGATAGTGATTTTATTGGTAGTGTTGTGCCCATGAGAGTAACGAATGAGAAAAAAAGGGTTAGGAAAAGGAGGAGAATGTCCGTACCTTCAGATTCAGATTTTGTGCCTTCTGGAGCATCTGATTTTGACTACACCATCTCTGAGGAAGAGAGAGAGCAAGTACGAGAAGCCAAGGAGTTGTGTGAAAGATTGGAAACTAATTTGCGAAGCTCATCAGTGCCTACTAGCCTTCCGGAGGATACACTTGTACAGCTGCCAAGAAAGCCTCCTAGAAGGAAGGGCAAAGAGAAGATTGAACAAGTGAAGGTTGAGGTTGTAAAGCAGGTCTGTGGAATTTGTTTTTCCGAGGAAGATAAAAGAAGAGTGAGGGGAACATTAGACTGTTGCACTCATTATTTTTGTTTTGCATGCATCATGGAATGGGGAAAAGTGGAATCTCGTTGCCCTTTGTGCAAGCAAAGGTTTAAGACAATTAGTAAGCCTGCAAGATCTACGACCGGAATTGATTTGAGAGAAGTGGTGATACAGGTCCCTGAGCGTGATCAGGTCTATCAACCAACAGAAGAAGAACTCAGGAGTTATCTCGACCCTTATGAGAATGTGATCGATCTCGATGTACAGAATGCCACGAAGGAGGGGAAGATGGCCTCATGCTACTGTGTGATATATGTGATTCACCTGCTCACACCTTTTGTGTTGGTCTTGGTCGAGAAGTACCTGATGGTAATTGGTACTGTGAGGGTTGTAGACCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006325 GO:0006338 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008623 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016590 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019899 GO:0019904 GO:0031010 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070063 GO:0070603 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1903506 GO:1904949 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PHD zf-RING_2

Protein Analysis

536

Amino Acids

62.01

Weight (kDa)

5.81

Isoelectric Point (pI)

64.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	393 - 436	1.9e-06	Ring finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006287)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05670 AT3G05670 AT3G05670
fragaria_vesca	FvH4_6g07080
malus_domestica	MD04G1196300.v1.1 MD12G1209300.v1.1
prunus_persica	Prupe.6G316500_v2.0.a1 Prupe.6G316500_v2.0.a1 Prupe.6G316500_v2.0.a1 Prupe.6G316500_v2.0.a1
pyrus_communis	pycom04g17360 pycom12g19660
rosa_chinensis	RchiOBHm_Chr3g0456661
rosa_laevigata	RLG00000025286
rosa_multiflora	Rmu_co8126688.1_g000001 Rmu_sc0000133.1_g000031
rosa_roxburghii	Rroxscaffold_11G00450420 Rroxscaffold_6G00422560
rosa_rugosa	Rorug03G0014500 Rorug03G0014600 Rorug03G0014700 Rorug03G0014800.1
rosa_samantha	Rh3AG075300 Rh3BG077200 Rh3CG076900 Rh3DG078900
rosa_wichuraiana	Rw3G006450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	597
AarI	CACCTGC	1 cut(s)	1539
Acc36I	ACCTGC	2 cut(s)	1165, 1539
AccI	GTMKAC	1 cut(s)	1599
AccII	CGCG	1 cut(s)	10
AccIII	TCCGGA	1 cut(s)	1084
AciI	CCGC	3 cut(s)	332, 712, 715
AclWI	GGATC	1 cut(s)	594
AcsI	RAATTY	3 cut(s)	626, 647, 1185
AcuI	CTGAAG	1 cut(s)	924
AfaI	GTAC	6 cut(s)	936, 1014, 1101, 1470, 1568, 1585
AfiI	CCNNNNNNNGG	1 cut(s)	1087
AgeI	ACCGGT	1 cut(s)	475
AgsI	TTSAA	3 cut(s)	721, 848, 1148
AjuI	GAANNNNNNNTTGG	2 cut(s)	303, 335
AloI	GAACNNNNNNTCC	2 cut(s)	145, 177
AluBI	AGCT	3 cut(s)	318, 1062, 1105
AluI	AGCT	3 cut(s)	318, 1062, 1105
Alw21I	GWGCWC	1 cut(s)	99
AlwI	GGATC	1 cut(s)	594
Ama87I	CYCGRG	1 cut(s)	34
Aor13HI	TCCGGA	1 cut(s)	1084
AoxI	GGCC	1 cut(s)	1497
ApeKI	GCWGC	1 cut(s)	1105
ApoI	RAATTY	3 cut(s)	626, 647, 1185
AsiGI	ACCGGT	1 cut(s)	475
AspS9I	GGNCC	2 cut(s)	73, 1381
AsuHPI	GGTGA	5 cut(s)	379, 688, 758, 1384, 1520
AvaI	CYCGRG	1 cut(s)	34
AvaII	GGWCC	2 cut(s)	73, 1381
BaeGI	GKGCMC	1 cut(s)	882
BaeI	ACNNNNGTAYC	2 cut(s)	1083, 1116
BarI	GAAGNNNNNNTAC	2 cut(s)	1359, 1391
BbsI	GAAGAC	2 cut(s)	318, 462
Bbv12I	GWGCWC	1 cut(s)	99
BbvI	GCAGC	1 cut(s)	1092
BccI	CCATC	6 cut(s)	133, 290, 808, 995, 1489, 1567
BciVI	GTATCC	1 cut(s)	1084
BclI	TGATCA	1 cut(s)	1393
BfaI	CTAG	2 cut(s)	1077, 1122
BfmI	CTRYAG	1 cut(s)	1604
BfuAI	ACCTGC	2 cut(s)	1165, 1539
BfuI	GTATCC	1 cut(s)	1084
BglII	AGATCT	1 cut(s)	1340
BisI	GCNGC	2 cut(s)	713, 1106
BlsI	GCNGC	2 cut(s)	714, 1107
Bme18I	GGWCC	2 cut(s)	73, 1381
BmeT110I	CYCGRG	1 cut(s)	34
BmgT120I	GGNCC	2 cut(s)	73, 1381
BmiI	GGNNCC	2 cut(s)	208, 1383
BmrI	ACTGGG	1 cut(s)	713
BmsI	GCATC	3 cut(s)	292, 977, 1272
BmuI	ACTGGG	1 cut(s)	713
BpiI	GAAGAC	2 cut(s)	318, 462
BpmI	CTGGAG	1 cut(s)	984
Bpu10I	CCTNAGC	1 cut(s)	1386
Bsa29I	ATCGAT	1 cut(s)	1458
BsaJI	CCNNGG	2 cut(s)	1023, 1197
BsaWI	WCCGGW	4 cut(s)	91, 475, 1084, 1349
BsaXI	ACNNNNNCTCC	4 cut(s)	46, 76, 141, 171
Bsc4I	CCNNNNNNNGG	1 cut(s)	1087
Bse118I	RCCGGY	1 cut(s)	475
Bse1I	ACTGG	1 cut(s)	708
BseAI	TCCGGA	1 cut(s)	1084
BseCI	ATCGAT	1 cut(s)	1458
BseDI	CCNNGG	2 cut(s)	1023, 1197
BseGI	GGATG	3 cut(s)	226, 301, 841
BseLI	CCNNNNNNNGG	1 cut(s)	1087
BseMII	CTCAG	5 cut(s)	124, 252, 984, 1377, 1438
BseNI	ACTGG	1 cut(s)	708
BseSI	GKGCMC	1 cut(s)	882
BseXI	GCAGC	1 cut(s)	1092
Bsh1236I	CGCG	1 cut(s)	10
Bsh1285I	CGRYCG	1 cut(s)	1350
BshFI	GGCC	1 cut(s)	1499
BshTI	ACCGGT	1 cut(s)	475
BshVI	ATCGAT	1 cut(s)	1458
BsiEI	CGRYCG	1 cut(s)	1350
BsiHKAI	GWGCWC	1 cut(s)	99
BsiHKCI	CYCGRG	1 cut(s)	34
BsiSI	CCGG	4 cut(s)	92, 476, 1085, 1350
BslFI	GGGAC	1 cut(s)	1367
BslI	CCNNNNNNNGG	1 cut(s)	1087
BsmFI	GGGAC	1 cut(s)	1367
BsmI	GAATGC	1 cut(s)	1480
BsnI	GGCC	1 cut(s)	1499
BsoBI	CYCGRG	1 cut(s)	34
Bsp1286I	GDGCHC	2 cut(s)	99, 882
Bsp13I	TCCGGA	1 cut(s)	1084
Bsp1407I	TGTACA	2 cut(s)	1099, 1468
Bsp143I	GATC	6 cut(s)	383, 586, 1340, 1393, 1455, 1459
BspACI	CCGC	3 cut(s)	332, 712, 715
BspANI	GGCC	1 cut(s)	1499
BspCNI	CTCAG	5 cut(s)	123, 253, 985, 1378, 1437
BspDI	ATCGAT	1 cut(s)	1458
BspEI	TCCGGA	1 cut(s)	1084
BspFNI	CGCG	1 cut(s)	10
BspLI	GGNNCC	2 cut(s)	208, 1383
BspMI	ACCTGC	2 cut(s)	1165, 1539
BspPI	GGATC	1 cut(s)	594
BsrFI	RCCGGY	1 cut(s)	475
BsrGI	TGTACA	2 cut(s)	1099, 1468
BsrI	ACTGG	1 cut(s)	708
BssAI	RCCGGY	1 cut(s)	475
BssECI	CCNNGG	2 cut(s)	1023, 1197
BssMI	GATC	6 cut(s)	383, 586, 1340, 1393, 1455, 1459
BssT1I	CCWWGG	1 cut(s)	1023
Bst4CI	ACNGT	7 cut(s)	62, 284, 380, 546, 1235, 1511, 1588
Bst6I	CTCTTC	3 cut(s)	339, 993, 1207
BstAUI	TGTACA	2 cut(s)	1099, 1468
BstC8I	GCNNGC	5 cut(s)	99, 710, 1261, 1307, 1335
BstDEI	CTNAG	5 cut(s)	110, 261, 993, 1386, 1424
BstENI	CCTNNNNNAGG	1 cut(s)	1085
BstF5I	GGATG	3 cut(s)	226, 301, 841
BstFNI	CGCG	1 cut(s)	10
BstKTI	GATC	6 cut(s)	386, 589, 1343, 1396, 1458, 1462
BstMBI	GATC	6 cut(s)	383, 586, 1340, 1393, 1455, 1459
BstMCI	CGRYCG	1 cut(s)	1350
BstMWI	GCNNNNNNNGC	2 cut(s)	1114, 1302
BstNSI	RCATGY	1 cut(s)	1263
BstSFI	CTRYAG	1 cut(s)	1604
BstSLI	GKGCMC	1 cut(s)	882
BstUI	CGCG	1 cut(s)	10
BstV1I	GCAGC	1 cut(s)	1092
BstV2I	GAAGAC	2 cut(s)	318, 462
BstX2I	RGATCY	1 cut(s)	1340
BstYI	RGATCY	1 cut(s)	1340
Bsu15I	ATCGAT	1 cut(s)	1458
BsuI	GTATCC	1 cut(s)	1084
BsuRI	GGCC	1 cut(s)	1499
BsuTUI	ATCGAT	1 cut(s)	1458
BtgZI	GCGATG	1 cut(s)	827
BtsCI	GGATG	3 cut(s)	226, 301, 841
BtsIMutI	CAGTG	1 cut(s)	1074
BveI	ACCTGC	2 cut(s)	1165, 1539
Cac8I	GCNNGC	5 cut(s)	99, 710, 1261, 1307, 1335
Cfr10I	RCCGGY	1 cut(s)	475
Cfr13I	GGNCC	2 cut(s)	73, 1381
ClaI	ATCGAT	1 cut(s)	1458
CseI	GACGC	1 cut(s)	294
Csp6I	GTAC	6 cut(s)	935, 1013, 1100, 1469, 1567, 1584
CspAI	ACCGGT	1 cut(s)	475
CviAII	CATG	4 cut(s)	883, 1260, 1267, 1503
CviQI	GTAC	6 cut(s)	935, 1013, 1100, 1469, 1567, 1584
DdeI	CTNAG	5 cut(s)	110, 261, 993, 1386, 1424
DpnI	GATC	6 cut(s)	385, 588, 1342, 1395, 1457, 1461
DpnII	GATC	6 cut(s)	383, 586, 1340, 1393, 1455, 1459
DraI	TTTAAA	1 cut(s)	774
Eam1104I	CTCTTC	3 cut(s)	339, 993, 1207
EarI	CTCTTC	3 cut(s)	339, 993, 1207
Eco130I	CCWWGG	1 cut(s)	1023
Eco47I	GGWCC	2 cut(s)	73, 1381
Eco57I	CTGAAG	1 cut(s)	924
Eco88I	CYCGRG	1 cut(s)	34
EcoNI	CCTNNNNNAGG	1 cut(s)	1085
EcoO109I	RGGNCCY	1 cut(s)	1381
EcoT14I	CCWWGG	1 cut(s)	1023
EcoT22I	ATGCAT	1 cut(s)	1265
ErhI	CCWWGG	1 cut(s)	1023
FaeI	CATG	4 cut(s)	886, 1263, 1270, 1506
FaqI	GGGAC	1 cut(s)	1367
FatI	CATG	4 cut(s)	882, 1259, 1266, 1502
FbaI	TGATCA	1 cut(s)	1393
FblI	GTMKAC	1 cut(s)	1599
Fnu4HI	GCNGC	2 cut(s)	713, 1106
FokI	GGATG	3 cut(s)	233, 308, 848
Fsp4HI	GCNGC	2 cut(s)	713, 1106
FspBI	CTAG	2 cut(s)	1077, 1122
GluI	GCNGC	2 cut(s)	713, 1106
GsuI	CTGGAG	1 cut(s)	984
HaeIII	GGCC	1 cut(s)	1499
HapII	CCGG	4 cut(s)	92, 476, 1085, 1350
HgaI	GACGC	1 cut(s)	294
Hin1II	CATG	4 cut(s)	886, 1263, 1270, 1506
HinfI	GANTC	7 cut(s)	187, 215, 489, 805, 944, 1286, 1525
HpaII	CCGG	4 cut(s)	92, 476, 1085, 1350
HphI	GGTGA	5 cut(s)	379, 688, 758, 1384, 1520
Hpy166II	GTNNAC	1 cut(s)	1600
Hpy8I	GTNNAC	1 cut(s)	1600
HpyCH4III	ACNGT	7 cut(s)	62, 284, 380, 546, 1235, 1511, 1588
HpyCH4V	TGCA	6 cut(s)	569, 1239, 1259, 1263, 1305, 1337
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1114, 1302
HpyF3I	CTNAG	5 cut(s)	110, 261, 993, 1386, 1424
Hsp92II	CATG	4 cut(s)	886, 1263, 1270, 1506
Kpn2I	TCCGGA	1 cut(s)	1084
Ksp22I	TGATCA	1 cut(s)	1393
Kzo9I	GATC	6 cut(s)	383, 586, 1340, 1393, 1455, 1459
LmnI	GCTCC	1 cut(s)	965
Lsp1109I	GCAGC	1 cut(s)	1092
LweI	GCATC	3 cut(s)	292, 977, 1272
MaeI	CTAG	2 cut(s)	1077, 1122
MaeIII	GTNAC	2 cut(s)	546, 889
MalI	GATC	6 cut(s)	385, 588, 1342, 1395, 1457, 1461
MboI	GATC	6 cut(s)	383, 586, 1340, 1393, 1455, 1459
MflI	RGATCY	1 cut(s)	1340
MhlI	GDGCHC	2 cut(s)	99, 882
MluCI	AATT	8 cut(s)	414, 626, 647, 1051, 1185, 1323, 1353, 1578
MlyI	GAGTC	1 cut(s)	814
Mph1103I	ATGCAT	1 cut(s)	1265
MroI	TCCGGA	1 cut(s)	1084
MseI	TTAA	4 cut(s)	413, 540, 773, 1317
MspA1I	CMGCKG	1 cut(s)	1105
MspI	CCGG	4 cut(s)	92, 476, 1085, 1350
Mva1269I	GAATGC	1 cut(s)	1480
MvnI	CGCG	1 cut(s)	10
MwoI	GCNNNNNNNGC	2 cut(s)	1114, 1302
NdeII	GATC	6 cut(s)	383, 586, 1340, 1393, 1455, 1459
NlaIII	CATG	4 cut(s)	886, 1263, 1270, 1506
NlaIV	GGNNCC	2 cut(s)	208, 1383
NsiI	ATGCAT	1 cut(s)	1265
NspI	RCATGY	1 cut(s)	1263
PaeI	GCATGC	1 cut(s)	1263
PaeR7I	CTCGAG	1 cut(s)	34
PaqCI	CACCTGC	1 cut(s)	1539
PcsI	WCGNNNNNNNCGW	1 cut(s)	809
PctI	GAATGC	1 cut(s)	1480
PfeI	GAWTC	6 cut(s)	187, 215, 489, 944, 1286, 1525
PinAI	ACCGGT	1 cut(s)	475
PkrI	GCNGC	2 cut(s)	714, 1107
PleI	GAGTC	1 cut(s)	813
PpsI	GAGTC	1 cut(s)	813
PpuMI	RGGWCCY	1 cut(s)	1381
PsiI	TTATAA	1 cut(s)	597
Psp5II	RGGWCCY	1 cut(s)	1381
PspN4I	GGNNCC	2 cut(s)	208, 1383
PspPI	GGNCC	2 cut(s)	73, 1381
PspPPI	RGGWCCY	1 cut(s)	1381
PsuI	RGATCY	1 cut(s)	1340
PvuII	CAGCTG	1 cut(s)	1105
RsaI	GTAC	6 cut(s)	936, 1014, 1101, 1470, 1568, 1585
RsaNI	GTAC	6 cut(s)	935, 1013, 1100, 1469, 1567, 1584
SaqAI	TTAA	4 cut(s)	413, 540, 773, 1317
SatI	GCNGC	2 cut(s)	713, 1106
Sau3AI	GATC	6 cut(s)	383, 586, 1340, 1393, 1455, 1459
Sau96I	GGNCC	2 cut(s)	73, 1381
SchI	GAGTC	1 cut(s)	814
SduI	GDGCHC	2 cut(s)	99, 882
SfaNI	GCATC	3 cut(s)	292, 977, 1272
SfcI	CTRYAG	1 cut(s)	1604
Sfr274I	CTCGAG	1 cut(s)	34
SinI	GGWCC	2 cut(s)	73, 1381
SlaI	CTCGAG	1 cut(s)	34
SmlI	CTYRAG	1 cut(s)	34
SmoI	CTYRAG	1 cut(s)	34
SphI	GCATGC	1 cut(s)	1263
Sse9I	AATT	8 cut(s)	414, 626, 647, 1051, 1185, 1323, 1353, 1578
SsiI	CCGC	3 cut(s)	332, 712, 715
SspMI	CTAG	2 cut(s)	1077, 1122
StyI	CCWWGG	1 cut(s)	1023
TaaI	ACNGT	7 cut(s)	62, 284, 380, 546, 1235, 1511, 1588
TaqI	TCGA	5 cut(s)	35, 1437, 1458, 1464, 1560
TasI	AATT	8 cut(s)	414, 626, 647, 1051, 1185, 1323, 1353, 1578
TatI	WGTACW	2 cut(s)	1099, 1468
TauI	GCSGC	1 cut(s)	715
TfiI	GAWTC	6 cut(s)	187, 215, 489, 944, 1286, 1525
Tru1I	TTAA	4 cut(s)	413, 540, 773, 1317
Tru9I	TTAA	4 cut(s)	413, 540, 773, 1317
TscAI	CASTG	1 cut(s)	1074
TseI	GCWGC	1 cut(s)	1105
TspDTI	ATGAA	5 cut(s)	267, 618, 654, 660, 855
TspGWI	ACGGA	2 cut(s)	180, 922
TspRI	CASTG	1 cut(s)	1074
VpaK11BI	GGWCC	2 cut(s)	73, 1381
XagI	CCTNNNNNAGG	1 cut(s)	1085
XapI	RAATTY	3 cut(s)	626, 647, 1185
XceI	RCATGY	1 cut(s)	1263
XhoI	CTCGAG	1 cut(s)	34
XmiI	GTMKAC	1 cut(s)	1599
XspI	CTAG	2 cut(s)	1077, 1122
Zsp2I	ATGCAT	1 cut(s)	1265

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.