pycom12g20150

protease Do-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005022
Physical Location & Seq
Forward (+)
21762701 .. 21764833
2133 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
ATGAGCCATTTTCTGAGAAAATTTTCAACTCGGAACCGGATTGCCGCCATCGCCGCTGCAGGGTCTGCTTTTTTGTACACCAACGGCAGCAGAAACTCCGACTATACGGCTTCAGTGTCAATCCCTGCGCCATTGCCCGAATCGCTGTGGCTTCCATGGCAAACGGAGCTATTGGCTCCACTATTTTCTGTGGGAAGTAGACCAGTTCCACCATCAGATATAAGGAAGGATATTTATGGTGTTTCCCCATCTGAAGAGACCCCAAAGCCATGTTCTGGATGTTTGGGCAGAGATTCTTTTGCAAAGGCGGCTGCGAAGGTTGGTCCTGCTGTTGTCAACATCTCCGCTCCACAGGGTGCATATGGGATCACTCCGGGAAAGAGTATAGGCTCTGGAACTATTATTGGCGAGGACGGTACAATTTTAACATGTGCTCATAGTGTAGTTGATTTTTATGGCCTGAGAGCTTCATCAAAGGGGAAGGTTCATATCACTTTGCAAGATGGTAGAACATTTGAGGGCACCGTGGTCAATGCAGATTTACAATCGGATGTGGCTATAGTAAAGATCAATTCTAAAACCCCACTGCCAACAGCAAAGCTCGGCAGTTCGCGTAAACTTCAACCTGGGGATTGGGTGATAGCTGTTGGTTGTCCACTTTCACTTCAGAATACGGTCACAGCTGGTATTATAAGTTGTGTTGACCGAAAGAGCACTGATTTGGGTCTTGGTGGAGTGCGGAGAGAGTATTTACAGACAGATTGCGCAATCAATCCGGGCAATTCTGGGGGGCCATTGGTGAATATTGACGGAGAAGTGATTGGTGTTAATATTATGAAAGTACTTGCTGCTGATGGATTGAGTTTTGCCTTCCCAATTGATTCAGTCACCAAAATCATGGAGCACTTCAAGAAAAGCGGGAGGGTTGTTCGGCCTTGGGTTGGACTAAAGATGATCGATCTCAATGAGATGTCATCAGTCAGCTTAAAGAAAGAGATCCTACATTCCCAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04210 ko04214 ko04215 ko05012 map04210 map04214 map04215 map05012
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

339

Amino Acids

35.75

Weight (kDa)

9.07

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 110 - 295 1.7e-17 Trypsin
Trypsin_2 PF13365 130 - 276 9.6e-33 Trypsin-like peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 692
Acc16I TGCGCA 1 cut(s) 766
AccB1I GGYRCC 1 cut(s) 521
AccB7I CCANNNNNTGG 1 cut(s) 275
AccBSI CCGCTC 1 cut(s) 347
AccI GTMKAC 1 cut(s) 199
AccII CGCG 1 cut(s) 613
AciI CCGC 6 cut(s) 45, 54, 308, 345, 739, 918
AclWI GGATC 2 cut(s) 374, 991
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 3 cut(s) 96, 273, 650
AdeI CACNNNGTG 1 cut(s) 356
AfaI GTAC 3 cut(s) 77, 418, 843
AfiI CCNNNNNNNGG 3 cut(s) 60, 275, 941
AflIII ACRYGT 1 cut(s) 428
AgsI TTSAA 3 cut(s) 27, 623, 910
AjnI CCWGG 1 cut(s) 625
AjuI GAANNNNNNNTTGG 2 cut(s) 256, 288
AluBI AGCT 6 cut(s) 169, 467, 601, 644, 683, 984
AluI AGCT 6 cut(s) 169, 467, 601, 644, 683, 984
Alw21I GWGCWC 3 cut(s) 436, 716, 906
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 2 cut(s) 374, 991
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 3 cut(s) 457, 791, 932
ApeKI GCWGC 4 cut(s) 56, 87, 311, 848
ApoI RAATTY 1 cut(s) 20
Asp700I GAANNNNTTC 1 cut(s) 22
AspLEI GCGC 2 cut(s) 130, 767
AspS9I GGNCC 2 cut(s) 323, 791
AsuC2I CCSGG 2 cut(s) 375, 777
AsuHPI GGTGA 3 cut(s) 649, 811, 880
AvaII GGWCC 1 cut(s) 323
BaeGI GKGCMC 1 cut(s) 524
BanI GGYRCC 1 cut(s) 521
Bbv12I GWGCWC 3 cut(s) 436, 716, 906
BbvI GCAGC 4 cut(s) 43, 99, 298, 835
BccI CCATC 5 cut(s) 56, 220, 256, 497, 848
BceAI ACGGC 2 cut(s) 100, 123
BciT130I CCWGG 1 cut(s) 627
BcnI CCSGG 2 cut(s) 375, 777
BcoDI GTCTC 1 cut(s) 251
BfmI CTRYAG 2 cut(s) 57, 558
BisI GCNGC 7 cut(s) 45, 54, 57, 88, 309, 312, 849
BlsI GCNGC 7 cut(s) 46, 55, 58, 89, 310, 313, 850
BmcAI AGTACT 1 cut(s) 843
Bme1390I CCNGG 3 cut(s) 375, 627, 777
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 2 cut(s) 323, 791
BmiI GGNNCC 4 cut(s) 35, 177, 523, 792
BmrFI CCNGG 3 cut(s) 375, 627, 777
BpuMI CCSGG 2 cut(s) 375, 777
Bsa29I ATCGAT 1 cut(s) 957
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 4 cut(s) 155, 525, 626, 935
BsaWI WCCGGW 1 cut(s) 36
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 275, 941
Bse1I ACTGG 1 cut(s) 203
Bse3DI GCAATG 1 cut(s) 131
BseBI CCWGG 1 cut(s) 627
BseCI ATCGAT 1 cut(s) 957
BseDI CCNNGG 4 cut(s) 155, 525, 626, 935
BseGI GGATG 2 cut(s) 284, 556
BseLI CCNNNNNNNGG 3 cut(s) 60, 275, 941
BseMI GCAATG 1 cut(s) 131
BseMII CTCAG 2 cut(s) 5, 452
BseNI ACTGG 1 cut(s) 203
BseSI GKGCMC 1 cut(s) 524
BseXI GCAGC 4 cut(s) 43, 99, 298, 835
Bsh1236I CGCG 1 cut(s) 613
BshFI GGCC 3 cut(s) 459, 793, 934
BshNI GGYRCC 1 cut(s) 521
BshVI ATCGAT 1 cut(s) 957
BsiHKAI GWGCWC 3 cut(s) 436, 716, 906
BsiSI CCGG 3 cut(s) 37, 374, 776
BslI CCNNNNNNNGG 3 cut(s) 60, 275, 941
BsmAI GTCTC 1 cut(s) 251
BsnI GGCC 3 cut(s) 459, 793, 934
Bso31I GGTCTC 1 cut(s) 251
Bsp1286I GDGCHC 4 cut(s) 436, 524, 716, 906
Bsp1407I TGTACA 1 cut(s) 75
Bsp143I GATC 5 cut(s) 366, 567, 954, 958, 996
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 6 cut(s) 45, 54, 308, 345, 739, 918
BspANI GGCC 3 cut(s) 459, 793, 934
BspCNI CTCAG 2 cut(s) 6, 453
BspDI ATCGAT 1 cut(s) 957
BspFNI CGCG 1 cut(s) 613
BspLI GGNNCC 4 cut(s) 35, 177, 523, 792
BspMAI CTGCAG 1 cut(s) 61
BspPI GGATC 2 cut(s) 374, 991
BspT107I GGYRCC 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 251
BsrBI CCGCTC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 75
BsrI ACTGG 1 cut(s) 203
BssECI CCNNGG 4 cut(s) 155, 525, 626, 935
BssMI GATC 5 cut(s) 366, 567, 954, 958, 996
BssT1I CCWWGG 2 cut(s) 155, 935
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 3 cut(s) 416, 526, 676
Bst6I CTCTTC 1 cut(s) 249
BstAPI GCANNNNNTGC 1 cut(s) 65
BstAUI TGTACA 1 cut(s) 75
BstDEI CTNAG 2 cut(s) 14, 461
BstDSI CCRYGG 2 cut(s) 155, 525
BstF5I GGATG 2 cut(s) 284, 556
BstFNI CGCG 1 cut(s) 613
BstHHI GCGC 2 cut(s) 130, 767
BstKTI GATC 5 cut(s) 369, 570, 957, 961, 999
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 5 cut(s) 366, 567, 954, 958, 996
BstMWI GCNNNNNNNGC 7 cut(s) 50, 53, 65, 142, 157, 166, 308
BstNI CCWGG 1 cut(s) 627
BstNSI RCATGY 1 cut(s) 432
BstSCI CCNGG 3 cut(s) 373, 625, 775
BstSFI CTRYAG 2 cut(s) 57, 558
BstSLI GKGCMC 1 cut(s) 524
BstUI CGCG 1 cut(s) 613
BstV1I GCAGC 4 cut(s) 43, 99, 298, 835
BstX2I RGATCY 1 cut(s) 996
BstXI CCANNNNNNTGG 1 cut(s) 898
BstYI RGATCY 1 cut(s) 996
Bsu15I ATCGAT 1 cut(s) 957
BsuRI GGCC 3 cut(s) 459, 793, 934
BsuTUI ATCGAT 1 cut(s) 957
BtgI CCRYGG 2 cut(s) 155, 525
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 2 cut(s) 284, 556
BtsI GCAGTG 1 cut(s) 584
BtsIMutI CAGTG 3 cut(s) 120, 584, 714
CaiI CAGNNNCTG 1 cut(s) 65
CfoI GCGC 2 cut(s) 130, 767
Cfr13I GGNCC 2 cut(s) 323, 791
ClaI ATCGAT 1 cut(s) 957
Csp6I GTAC 3 cut(s) 76, 417, 842
CviAII CATG 4 cut(s) 156, 270, 429, 898
CviQI GTAC 3 cut(s) 76, 417, 842
DdeI CTNAG 2 cut(s) 14, 461
DpnI GATC 5 cut(s) 368, 569, 956, 960, 998
DpnII GATC 5 cut(s) 366, 567, 954, 958, 996
DraIII CACNNNGTG 1 cut(s) 356
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 2 cut(s) 155, 935
Eco31I GGTCTC 1 cut(s) 251
Eco47I GGWCC 1 cut(s) 323
Eco57I CTGAAG 3 cut(s) 96, 273, 650
EcoRII CCWGG 1 cut(s) 625
EcoT14I CCWWGG 2 cut(s) 155, 935
ErhI CCWWGG 2 cut(s) 155, 935
FaeI CATG 4 cut(s) 159, 273, 432, 901
FatI CATG 4 cut(s) 155, 269, 428, 897
FauI CCCGC 1 cut(s) 911
FauNDI CATATG 1 cut(s) 361
FblI GTMKAC 1 cut(s) 199
Fnu4HI GCNGC 7 cut(s) 45, 54, 57, 88, 309, 312, 849
FokI GGATG 2 cut(s) 291, 563
Fsp4HI GCNGC 7 cut(s) 45, 54, 57, 88, 309, 312, 849
FspI TGCGCA 1 cut(s) 766
GlaI GCGC 2 cut(s) 129, 766
GluI GCNGC 7 cut(s) 45, 54, 57, 88, 309, 312, 849
HaeIII GGCC 3 cut(s) 459, 793, 934
HapII CCGG 3 cut(s) 37, 374, 776
HhaI GCGC 2 cut(s) 130, 767
Hin1II CATG 4 cut(s) 159, 273, 432, 901
Hin6I GCGC 2 cut(s) 128, 765
HinP1I GCGC 2 cut(s) 128, 765
HincII GTYRAC 2 cut(s) 337, 703
HindII GTYRAC 2 cut(s) 337, 703
HinfI GANTC 3 cut(s) 140, 293, 881
HpaII CCGG 3 cut(s) 37, 374, 776
HphI GGTGA 3 cut(s) 649, 811, 880
Hpy166II GTNNAC 6 cut(s) 78, 200, 337, 617, 656, 703
Hpy188I TCNGA 7 cut(s) 15, 33, 100, 217, 253, 550, 669
Hpy188III TCNNGA 3 cut(s) 276, 393, 910
Hpy8I GTNNAC 6 cut(s) 78, 200, 337, 617, 656, 703
HpyAV CCTTC 4 cut(s) 220, 310, 475, 880
HpyCH4III ACNGT 3 cut(s) 416, 526, 676
HpyCH4V TGCA 5 cut(s) 59, 302, 359, 499, 536
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 53, 65, 142, 157, 166, 308
HpyF3I CTNAG 2 cut(s) 14, 461
Hsp92II CATG 4 cut(s) 159, 273, 432, 901
HspAI GCGC 2 cut(s) 128, 765
Kzo9I GATC 5 cut(s) 366, 567, 954, 958, 996
LmnI GCTCC 4 cut(s) 166, 181, 352, 901
Lsp1109I GCAGC 4 cut(s) 43, 99, 298, 835
MaeIII GTNAC 2 cut(s) 676, 886
MalI GATC 5 cut(s) 368, 569, 956, 960, 998
MbiI CCGCTC 1 cut(s) 347
MboI GATC 5 cut(s) 366, 567, 954, 958, 996
MboII GAAGA 1 cut(s) 266
MfeI CAATTG 1 cut(s) 876
MflI RGATCY 1 cut(s) 996
MhlI GDGCHC 4 cut(s) 436, 524, 716, 906
MluCI AATT 5 cut(s) 20, 420, 571, 781, 876
MmeI TCCRAC 2 cut(s) 123, 922
MnlI CCTC 3 cut(s) 403, 511, 915
MroXI GAANNNNTTC 1 cut(s) 22
MseI TTAA 3 cut(s) 425, 828, 986
MspA1I CMGCKG 2 cut(s) 56, 683
MspI CCGG 3 cut(s) 37, 374, 776
MspR9I CCNGG 3 cut(s) 375, 627, 777
MunI CAATTG 1 cut(s) 876
MvaI CCWGG 1 cut(s) 627
MvnI CGCG 1 cut(s) 613
MwoI GCNNNNNNNGC 7 cut(s) 50, 53, 65, 142, 157, 166, 308
NciI CCSGG 2 cut(s) 375, 777
NcoI CCATGG 1 cut(s) 155
NdeI CATATG 1 cut(s) 361
NdeII GATC 5 cut(s) 366, 567, 954, 958, 996
NlaIII CATG 4 cut(s) 159, 273, 432, 901
NlaIV GGNNCC 4 cut(s) 35, 177, 523, 792
NmeAIII GCCGAG 1 cut(s) 582
NmuCI GTSAC 2 cut(s) 676, 886
NsbI TGCGCA 1 cut(s) 766
NspI RCATGY 1 cut(s) 432
PciI ACATGT 1 cut(s) 428
PdmI GAANNNNTTC 1 cut(s) 22
PfeI GAWTC 3 cut(s) 140, 293, 881
PflMI CCANNNNNTGG 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 373
PkrI GCNGC 7 cut(s) 46, 55, 58, 89, 310, 313, 850
PscI ACATGT 1 cut(s) 428
PsiI TTATAA 1 cut(s) 692
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 4 cut(s) 35, 177, 523, 792
PspPI GGNCC 2 cut(s) 323, 791
PstI CTGCAG 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 65
PsuI RGATCY 1 cut(s) 996
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 3 cut(s) 77, 418, 843
RsaNI GTAC 3 cut(s) 76, 417, 842
SaqAI TTAA 3 cut(s) 425, 828, 986
SatI GCNGC 7 cut(s) 45, 54, 57, 88, 309, 312, 849
Sau3AI GATC 5 cut(s) 366, 567, 954, 958, 996
Sau96I GGNCC 2 cut(s) 323, 791
ScaI AGTACT 1 cut(s) 843
ScrFI CCNGG 3 cut(s) 375, 627, 777
SduI GDGCHC 4 cut(s) 436, 524, 716, 906
SetI ASST 9 cut(s) 171, 321, 469, 486, 603, 628, 646, 685, 986
SfcI CTRYAG 2 cut(s) 57, 558
SinI GGWCC 1 cut(s) 323
Sse9I AATT 5 cut(s) 20, 420, 571, 781, 876
SsiI CCGC 6 cut(s) 45, 54, 308, 345, 739, 918
SspI AATATT 2 cut(s) 805, 832
StyD4I CCNGG 3 cut(s) 373, 625, 775
StyI CCWWGG 2 cut(s) 155, 935
TaaI ACNGT 3 cut(s) 416, 526, 676
TaqI TCGA 1 cut(s) 957
TaqII GACCGA 1 cut(s) 720
TasI AATT 5 cut(s) 20, 420, 571, 781, 876
TatI WGTACW 2 cut(s) 75, 841
TauI GCSGC 3 cut(s) 47, 56, 311
TfiI GAWTC 3 cut(s) 140, 293, 881
Tru1I TTAA 3 cut(s) 425, 828, 986
Tru9I TTAA 3 cut(s) 425, 828, 986
TscAI CASTG 3 cut(s) 120, 591, 721
TseFI GTSAC 2 cut(s) 676, 886
TseI GCWGC 4 cut(s) 56, 87, 311, 848
Tsp45I GTSAC 2 cut(s) 676, 886
TspDTI ATGAA 3 cut(s) 459, 476, 851
TspGWI ACGGA 2 cut(s) 179, 825
TspRI CASTG 3 cut(s) 120, 591, 721
Van91I CCANNNNNTGG 1 cut(s) 275
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 20
XceI RCATGY 1 cut(s) 432
XmiI GTMKAC 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 22
ZrmI AGTACT 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.