Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0004063

Physical Location & Seq

Genomic Coordinates Reverse (-)

21897391 .. 21903920

Gene Structure 6530 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3228 bp

ATGGTGGCTTCGGACTCTTTCAGCGTACGGATCGTCTCCGTCGACTACTACATGGCGCCGCCGATGCCAGGCCTCGATATCTCTTACAGTAGCTTCCAAGGTGGGAAGGTGAATGAGGTGCCAGTTATAAGAATATTCGGCGCCACGCCTGCTGGTCAGAAGACTTGCTTGCACATTCATAGAGCATTACCGTACTTATATGTGCCATGCGCTGATATCCCGATTCAGCTGCACCAGAAAGGTGATTCGTACACACATGATGTAGCTCTTGCTATAGAGAAAGCTTTGAAGCTCAAAGGTAATGCTGGTTCAAAGCGACAACATGTACATAGTTGCAGCCTTGTCCAGGCTAGGAAGCTTTATGGTTACCACTCATCGGAGGAGTTGTTTGTGAAGATATACCTCTACTATCCTCATGATGTCTCTCGTGCTGCAAGTCTTCTTCTGGGAAGCTCAGTTTTTGATAAATGCTTGCAGCCTCATGAATCTCACATTCCCTTCATTCTTCAATTTATGGTTGACTACAACTTGTATGGGATGGGTCATCTACATGTATCAAAGATTAAATTCCGTCATCCAATACCAGATGTTTTCGTCCATAGAAGGTCTGTTTACAACGGACAGCCTAGTCAGGACATAGATAAAACGACTTGCATGTCTGCTGATTTGCAGGCAGATATACGTGGCCATCTGTCTTTGGGTTCACCTGTTTGGATATCGTCCACAATTCCAGTTGATTGGACATGGAATTCTTCTGGTGAATTTGAAGGTTCCTCAGATCCAGACATTAACTGCATTAAACGTCAAAGTATTTGTGAACTTGAGGGCGATTCTACTGTAGATGAGATTCTTAATCAGCAGCTTAAGATGTATACTTCTCTCTCGCAAACTTGTTCAGATGTGAAGATGGTTCAGTCACTTATACCAATTTTGGAGGAGTGTGAAAGGACTGGAATACATGAGGCAGCAATACCACCTGATCCTGCCAAACCACTTCCTGAAGATGCTTTGAAAACTCTCTCGGTTGGTCTTGAATTTGACAACAAGCTTATTAAAGTGCACAGTGAAGCTGAAAGTTCTCTGTGTCGAACTCAATTAAGGAAAGATGTAAGGTCTGTGGAGCAGATGGCATCTCGAAAAGATGAAGGAAACTTTGCTGGACCCCAAACTGATAATCTTATTGATGGAGAAATAATTGGATCTCTGCCAACACAAGATTCTACTAAAGATGGTGTTCCAAATGCCAAACTATCATTGAATAAGGAACTTCCATCATCACAAGTAATCCCGACGGTAAACATGAAGGTTACAGATAGTGAAGCACTAGGTCTTCTTAGATGGCTTGCTACTTCTCAGGCCGCTGATGATATAAACTCTGATGACGAACTTGTTTGTGAGACAATTCTTAGTCCCTTGTTGCCGGCAACAACCATTGACAATGTTCTAAACAAAGCTAATGTGGATTATGAGACTGAATCCCAAAAGGAGTGTCAGGACATACTTGATTCGGTTGGTGATATGATTGACTTTGAAAGATTGCAAGAAAGAGATTCTTACCCTTCAGATCGAAGGAATTCTTCAAAAAGTTCGTCTGAGTATGTGATTCACCAGGTGGATGGTTGTGGGGATGACGATTTCTCAACTCCTTGTGATGAATCAGTTGGAGGCTTTTCTGAAATAGAGGGGAAGAGTGAATTTAGGACCTCCAGCCATCAGGTGCAAGATGCTAGCTCCAGCTTCAATCATAAGCACAAAAGGAAAAAATTATGGGGCTCTTTACCTTTCTCTGAGACCTCAAAGATGAAGACAGAAAGGGAACATGCCCGTTTACACATAGAAACAAAGGACCCTAGTGGTACAAGTTCTTCTGAAAATGAAGTGGGAAAACGTGCAGGCACTGGAACTTGTGATGCAAAAGACAGTAGTATGTTGGCAAGATGCTCTGTGAGGGACTTGATGAGGAGAAAGCGATCCTACCGAGTTGAGCCACCTGAAAGTAGTGGGAGGAAGGAAAACGAAGACACACTTCTCTGCGCAAAGCGTTTAGATTTTCGAGTGTCAGGTACTGATGAGCATGACAGGAAGCCTCACGAGTCATCTAACTTCAGGCTATCATTAGCTGACCAACAATCAGGAAGTTATAAGGCTTATAAATATGATGCTAAACCAACCAATCCTGGTTGTTCAATGTATGGTAAACTTCCACTGTGTTCTAGTAATGACAACATTTTACATGCAAGTACGTTAGATGGAAAATTGGATTCCAATGTGTTGATTGGTGAAGAAGTTGGTGCTGGTGCAATGGCTCAATTTCAGAACTCCTTGATTACAGGCCCTTCTCAAGTTCCTTCTGTGAAAAAAAGAATGCAGACTTGGTCTGCTGAGCATGAAGTGGCTGATGGAGATGGTTATGTTGAAAGTGGCTTTGAAAGCTTATCTTCCAAGGGTGGAGTATATGAAATGTCTTTTCAGAATCCCATGGATGCAAATGCCACAACTGTCAGTGCTGTTCTAAACAATGAGAGGTCTGGTGTCCTAAAACTGGGTGGGGACTCTGTTTCATCTGGGCTCAGACACCCACCGTCTGAGGTCATCTATCCGGATGATGAGGACTTAATTGGAATGACATTCCACAAGAAGCCTCCTGTTCCAGACTGGAAGGACAGGGCATCTAAAGACGCTTCATCTTTATACGATGGAAGAGCTACGGATGACTATTTCCCCTTCTTTGTGGGAGATTTCCCAGACAAGAAAGAAATTCAGAACAAGTGTATTAGAAGTGAATCTAGCTCTCATGAAGAATCTGTTATGGGTGTCCCTACTCACTATCAAACTGATGGCTCTTACTTGTATTTGTTAACACCTGCAACTTCACCCCCTTCTGCCGAAAAGGTGAATAGATGGTTGTCAAGTGATGAAAAAGATGACATTCCACCTATTCTGCCCCAAGGACCTCAAGAGAATCATGAAAATCATGAAGAAGAAAGGAACCATTTATGCACTGACAGGACGGGAATAGCGCAAAGAGAAGGAGATGCTGTTAAAGTTCAAACAAACTCCGAATGCTCACAAGATAATTCTCAGATTTCTGGCCCAGATGGGAGATCAAAGCCCACTCCTCTTAGTCAAATTGGATTCAGGGACCCTGCAAGTGTTGGCGCAGGCCAACAGTTGACATTGTTAAGCATAGAGGTTCAAGCACAATCAAGAGGAGATCGTTCGACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000731 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010225 GO:0016035 GO:0018130 GO:0019438 GO:0019985 GO:0032991 GO:0033554 GO:0034641 GO:0034644 GO:0034645 GO:0034654 GO:0042276 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0071214 GO:0071478 GO:0071482 GO:0071494 GO:0071704 GO:0071897 GO:0090304 GO:0104004 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko01100 ko01524 ko03460 map01100 map01524 map03460

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 RVT_2 zf-C4pol zf-RanBP

Protein Analysis

1076

Amino Acids

118.67

Weight (kDa)

5.26

Isoelectric Point (pI)

53.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
REV3_N	PF24065	6 - 60	2.3e-19	DNA polymerase zeta catalytic subunit, N-terminal
POL3_N	PF24055	62 - 141	5.5e-23	DNA polymerase delta catalytic subunit-like, N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004063)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500
fragaria_vesca	FvH4_6g06120 FvH4_6g06120 FvH4_6g06120 FvH4_6g06120 FvH4_6g06120
malus_domestica	MD05G1053400.v1.1 MD05G1053600.v1.1 MD05G1053700.v1.1 MD12G1217900.v1.1
prunus_persica	Prupe.6G324000_v2.0.a1 Prupe.6G324000_v2.0.a1 Prupe.6G324000_v2.0.a1
pyrus_communis	pycom05g04420 pycom12g20340
rosa_chinensis	RchiOBHm_Chr3g0455131
rosa_laevigata	RLG00000025385
rosa_multiflora	Rmu_co8200746.1_g000001 Rmu_sc0041669.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424540
rosa_rugosa	Rorug01G0381700 Rorug03G0004000
rosa_samantha	Rh3AG064900 Rh3BG066900 Rh3CG065300 Rh3DG066800
rosa_wichuraiana	Rw3G005080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	128, 2142, 2151
AarI	CACCTGC	1 cut(s)	2872
AasI	GACNNNNNNGTC	2 cut(s)	627, 655
Acc16I	TGCGCA	1 cut(s)	2035
Acc36I	ACCTGC	1 cut(s)	2872
AccB1I	GGYRCC	3 cut(s)	55, 118, 140
AccI	GTMKAC	2 cut(s)	42, 872
AccIII	TCCGGA	1 cut(s)	2599
AciI	CCGC	2 cut(s)	59, 1359
AclWI	GGATC	5 cut(s)	38, 773, 974, 1207, 1965
AcoI	YGGCCR	1 cut(s)	685
AcsI	RAATTY	7 cut(s)	566, 748, 761, 1034, 1573, 1694, 2757
AcuI	CTGAAG	3 cut(s)	1020, 1545, 2089
AcyI	GRCGYC	2 cut(s)	56, 141
AdeI	CACNNNGTG	1 cut(s)	1612
AfaI	GTAC	7 cut(s)	27, 194, 251, 327, 1858, 2065, 2242
AfiI	CCNNNNNNNGG	4 cut(s)	68, 346, 376, 2542
AflII	CTTAAG	1 cut(s)	863
AflIII	ACRYGT	2 cut(s)	322, 550
AjnI	CCWGG	4 cut(s)	67, 345, 1608, 2176
AjuI	GAANNNNNNNTTGG	4 cut(s)	1201, 1233, 2356, 2388
Alw21I	GWGCWC	1 cut(s)	1062
Alw26I	GTCTC	5 cut(s)	40, 427, 1391, 1463, 1784
Alw44I	GTGCAC	1 cut(s)	1058
AlwI	GGATC	5 cut(s)	38, 773, 974, 1207, 1965
AlwNI	CAGNNNCTG	2 cut(s)	1898, 2066
Aor13HI	TCCGGA	1 cut(s)	2599
AoxI	GGCC	6 cut(s)	70, 685, 1356, 2332, 3091, 3163
ApaLI	GTGCAC	1 cut(s)	1058
ApeKI	GCWGC	6 cut(s)	229, 336, 431, 475, 859, 965
ApoI	RAATTY	7 cut(s)	566, 748, 761, 1034, 1573, 1694, 2757
ArsI	GACNNNNNNTTYG	4 cut(s)	1474, 1506, 1574, 1606
Asp700I	GAANNNNTTC	1 cut(s)	1573
AspLEI	GCGC	6 cut(s)	58, 143, 212, 2036, 3022, 3161
AspS9I	GGNCC	7 cut(s)	1160, 1701, 1846, 2333, 2951, 3092, 3142
AsuHPI	GGTGA	9 cut(s)	121, 254, 696, 770, 1526, 1598, 2291, 2865, 2905
AsuNHI	GCTAGC	1 cut(s)	1727
AvaII	GGWCC	5 cut(s)	1160, 1701, 1846, 2951, 3142
BaeGI	GKGCMC	1 cut(s)	1062
BalI	TGGCCA	1 cut(s)	687
BanI	GGYRCC	3 cut(s)	55, 118, 140
BanII	GRGCYC	2 cut(s)	1775, 2571
BarI	GAAGNNNNNNTAC	2 cut(s)	1849, 1881
BauI	CACGAG	2 cut(s)	426, 2090
BbsI	GAAGAC	5 cut(s)	167, 431, 1322, 1811, 2025
Bbv12I	GWGCWC	1 cut(s)	1062
BbvI	GCAGC	6 cut(s)	216, 348, 418, 487, 871, 977
BcgI	CGANNNNNNTGC	2 cut(s)	211, 245
BciT130I	CCWGG	4 cut(s)	69, 347, 1610, 2178
BcoDI	GTCTC	5 cut(s)	40, 427, 1391, 1463, 1784
BfaI	CTAG	7 cut(s)	351, 627, 1325, 1728, 1851, 2214, 2787
BfmI	CTRYAG	2 cut(s)	273, 837
BfoI	RGCGCY	2 cut(s)	59, 144
BfrI	CTTAAG	1 cut(s)	863
BfuAI	ACCTGC	1 cut(s)	2872
BisI	GCNGC	8 cut(s)	59, 230, 337, 432, 476, 860, 966, 1359
BlpI	GCTNAGC	1 cut(s)	2382
BlsI	GCNGC	8 cut(s)	60, 231, 338, 433, 477, 861, 967, 1360
Bme1390I	CCNGG	4 cut(s)	69, 347, 1610, 2178
Bme18I	GGWCC	5 cut(s)	1160, 1701, 1846, 2951, 3142
BmgT120I	GGNCC	7 cut(s)	1160, 1701, 1846, 2333, 2951, 3092, 3142
BmiI	GGNNCC	9 cut(s)	57, 120, 142, 772, 1162, 1848, 2990, 3143, 3144
BmrFI	CCNGG	4 cut(s)	69, 347, 1610, 2178
BmrI	ACTGGG	1 cut(s)	2552
BmtI	GCTAGC	1 cut(s)	1731
BmuI	ACTGGG	1 cut(s)	2552
BpiI	GAAGAC	5 cut(s)	167, 431, 1322, 1811, 2025
BpmI	CTGGAG	2 cut(s)	1690, 1717
Bpu1102I	GCTNAGC	1 cut(s)	2382
BpuEI	CTTGAG	3 cut(s)	842, 2325, 2940
BsaAI	YACGTR	1 cut(s)	683
BsaHI	GRCGYC	2 cut(s)	56, 141
BsaI	GGTCTC	1 cut(s)	1784
BsaJI	CCNNGG	4 cut(s)	97, 2442, 2478, 2947
BsaWI	WCCGGW	1 cut(s)	2599
BsaXI	ACNNNNNCTCC	4 cut(s)	371, 401, 3024, 3054
Bsc4I	CCNNNNNNNGG	4 cut(s)	68, 346, 376, 2542
Bse118I	RCCGGY	1 cut(s)	1420
Bse1I	ACTGG	6 cut(s)	122, 731, 955, 1903, 2547, 2660
Bse3DI	GCAATG	1 cut(s)	2307
BseAI	TCCGGA	1 cut(s)	2599
BseBI	CCWGG	4 cut(s)	69, 347, 1610, 2178
BseDI	CCNNGG	4 cut(s)	97, 2442, 2478, 2947
BseGI	GGATG	7 cut(s)	543, 574, 1621, 1633, 2488, 2608, 2716
BseLI	CCNNNNNNNGG	4 cut(s)	68, 346, 376, 2542
BseMI	GCAATG	1 cut(s)	2307
BseMII	CTCAG	9 cut(s)	468, 789, 1367, 1584, 1779, 2373, 2577, 2584, 3095
BseNI	ACTGG	6 cut(s)	122, 731, 955, 1903, 2547, 2660
BseRI	GAGGAG	5 cut(s)	395, 950, 1975, 3108, 3225
BseSI	GKGCMC	1 cut(s)	1062
BseXI	GCAGC	6 cut(s)	216, 348, 418, 487, 871, 977
BsgI	GTGCAG	2 cut(s)	215, 1911
BshFI	GGCC	6 cut(s)	72, 687, 1358, 2334, 3093, 3165
BshNI	GGYRCC	3 cut(s)	55, 118, 140
BsiHKAI	GWGCWC	1 cut(s)	1062
BsiSI	CCGG	2 cut(s)	1421, 2600
BsiWI	CGTACG	1 cut(s)	25
BslFI	GGGAC	5 cut(s)	1395, 1964, 2564, 2801, 3155
BslI	CCNNNNNNNGG	4 cut(s)	68, 346, 376, 2542
BsmAI	GTCTC	5 cut(s)	40, 427, 1391, 1463, 1784
BsmBI	CGTCTC	1 cut(s)	40
BsmFI	GGGAC	5 cut(s)	1395, 1964, 2564, 2801, 3155
BsmI	GAATGC	2 cut(s)	2370, 3068
BsnI	GGCC	6 cut(s)	72, 687, 1358, 2334, 3093, 3165
Bso31I	GGTCTC	1 cut(s)	1784
Bsp1286I	GDGCHC	3 cut(s)	1062, 1775, 2571
Bsp13I	TCCGGA	1 cut(s)	2599
Bsp1407I	TGTACA	1 cut(s)	325
Bsp143I	GATC	8 cut(s)	30, 778, 979, 1199, 1564, 1970, 3104, 3214
Bsp1720I	GCTNAGC	1 cut(s)	2382
Bsp19I	CCATGG	1 cut(s)	2478
BspACI	CCGC	2 cut(s)	59, 1359
BspANI	GGCC	6 cut(s)	72, 687, 1358, 2334, 3093, 3165
BspCNI	CTCAG	9 cut(s)	467, 788, 1366, 1585, 1780, 2374, 2578, 2583, 3094
BspEI	TCCGGA	1 cut(s)	2599
BspHI	TCATGA	5 cut(s)	415, 481, 2794, 2965, 2974
BspLI	GGNNCC	9 cut(s)	57, 120, 142, 772, 1162, 1848, 2990, 3143, 3144
BspMI	ACCTGC	1 cut(s)	2872
BspOI	GCTAGC	1 cut(s)	1731
BspPI	GGATC	5 cut(s)	38, 773, 974, 1207, 1965
BspQI	GCTCTTC	1 cut(s)	2695
BspT107I	GGYRCC	3 cut(s)	55, 118, 140
BspTI	CTTAAG	1 cut(s)	863
BspTNI	GGTCTC	1 cut(s)	1784
BsrDI	GCAATG	1 cut(s)	2307
BsrFI	RCCGGY	1 cut(s)	1420
BsrGI	TGTACA	1 cut(s)	325
BsrI	ACTGG	6 cut(s)	122, 731, 955, 1903, 2547, 2660
BssAI	RCCGGY	1 cut(s)	1420
BssECI	CCNNGG	4 cut(s)	97, 2442, 2478, 2947
BssMI	GATC	8 cut(s)	30, 778, 979, 1199, 1564, 1970, 3104, 3214
BssNAI	GTATAC	1 cut(s)	873
BssNI	GRCGYC	2 cut(s)	56, 141
BssSI	CACGAG	2 cut(s)	426, 2090
BssT1I	CCWWGG	4 cut(s)	97, 2442, 2478, 2947
Bst1107I	GTATAC	1 cut(s)	873
Bst2BI	CACGAG	2 cut(s)	426, 2090
Bst2UI	CCWGG	4 cut(s)	69, 347, 1610, 2178
Bst6I	CTCTTC	2 cut(s)	1682, 2695
BstACI	GRCGYC	2 cut(s)	56, 141
BstAFI	CTTAAG	1 cut(s)	863
BstAUI	TGTACA	1 cut(s)	325
BstBAI	YACGTR	1 cut(s)	683
BstC8I	GCNNGC	9 cut(s)	150, 170, 473, 672, 1344, 1422, 1729, 1894, 3163
BstDSI	CCRYGG	1 cut(s)	2478
BstEII	GGTNACC	1 cut(s)	365
BstENI	CCTNNNNNAGG	1 cut(s)	344
BstF5I	GGATG	7 cut(s)	543, 574, 1621, 1633, 2488, 2608, 2716
BstH2I	RGCGCY	2 cut(s)	59, 144
BstHHI	GCGC	6 cut(s)	58, 143, 212, 2036, 3022, 3161
BstKTI	GATC	8 cut(s)	33, 781, 982, 1202, 1567, 1973, 3107, 3217
BstMAI	GTCTC	5 cut(s)	40, 427, 1391, 1463, 1784
BstMBI	GATC	8 cut(s)	30, 778, 979, 1199, 1564, 1970, 3104, 3214
BstMWI	GCNNNNNNNGC	3 cut(s)	64, 149, 2430
BstNI	CCWGG	4 cut(s)	69, 347, 1610, 2178
BstNSI	RCATGY	5 cut(s)	326, 554, 658, 1823, 2237
BstPI	GGTNACC	1 cut(s)	365
BstSCI	CCNGG	4 cut(s)	67, 345, 1608, 2176
BstSFI	CTRYAG	2 cut(s)	273, 837
BstSLI	GKGCMC	1 cut(s)	1062
BstV1I	GCAGC	6 cut(s)	216, 348, 418, 487, 871, 977
BstV2I	GAAGAC	5 cut(s)	167, 431, 1322, 1811, 2025
BstX2I	RGATCY	2 cut(s)	778, 1199
BstXI	CCANNNNNNTGG	2 cut(s)	738, 1616
BstYI	RGATCY	2 cut(s)	778, 1199
BstZ17I	GTATAC	1 cut(s)	873
BsuRI	GGCC	6 cut(s)	72, 687, 1358, 2334, 3093, 3165
BtgI	CCRYGG	1 cut(s)	2478
BtsCI	GGATG	7 cut(s)	543, 574, 1621, 1633, 2488, 2608, 2716
BtsIMutI	CAGTG	5 cut(s)	1069, 1896, 2204, 2509, 3000
BveI	ACCTGC	1 cut(s)	2872
Cac8I	GCNNGC	9 cut(s)	150, 170, 473, 672, 1344, 1422, 1729, 1894, 3163
CaiI	CAGNNNCTG	2 cut(s)	1898, 2066
CciI	TCATGA	5 cut(s)	415, 481, 2794, 2965, 2974
CfoI	GCGC	6 cut(s)	58, 143, 212, 2036, 3022, 3161
Cfr10I	RCCGGY	1 cut(s)	1420
Cfr13I	GGNCC	7 cut(s)	1160, 1701, 1846, 2333, 2951, 3092, 3142
CseI	GACGC	1 cut(s)	2687
CsiI	ACCWGGT	1 cut(s)	1608
Csp6I	GTAC	7 cut(s)	26, 193, 250, 326, 1857, 2064, 2241
CviQI	GTAC	7 cut(s)	26, 193, 250, 326, 1857, 2064, 2241
DinI	GGCGCC	2 cut(s)	57, 142
DpnI	GATC	8 cut(s)	32, 780, 981, 1201, 1566, 1972, 3106, 3216
DpnII	GATC	8 cut(s)	30, 778, 979, 1199, 1564, 1970, 3104, 3214
DraIII	CACNNNGTG	1 cut(s)	1612
DrdI	GACNNNNNNGTC	2 cut(s)	627, 655
DseDI	GACNNNNNNGTC	2 cut(s)	627, 655
EaeI	YGGCCR	1 cut(s)	685
Eam1104I	CTCTTC	2 cut(s)	1682, 2695
EarI	CTCTTC	2 cut(s)	1682, 2695
Eco130I	CCWWGG	4 cut(s)	97, 2442, 2478, 2947
Eco147I	AGGCCT	1 cut(s)	72
Eco24I	GRGCYC	2 cut(s)	1775, 2571
Eco31I	GGTCTC	1 cut(s)	1784
Eco32I	GATATC	3 cut(s)	79, 217, 717
Eco47I	GGWCC	5 cut(s)	1160, 1701, 1846, 2951, 3142
Eco57I	CTGAAG	3 cut(s)	1020, 1545, 2089
Eco91I	GGTNACC	1 cut(s)	365
EcoNI	CCTNNNNNAGG	1 cut(s)	344
EcoO109I	RGGNCCY	5 cut(s)	1701, 1846, 2333, 2951, 3142
EcoO65I	GGTNACC	1 cut(s)	365
EcoRI	GAATTC	2 cut(s)	748, 1573
EcoRII	CCWGG	4 cut(s)	67, 345, 1608, 2176
EcoRV	GATATC	3 cut(s)	79, 217, 717
EcoT14I	CCWWGG	4 cut(s)	97, 2442, 2478, 2947
EcoT38I	GRGCYC	2 cut(s)	1775, 2571
EgeI	GGCGCC	2 cut(s)	57, 142
EheI	GGCGCC	2 cut(s)	57, 142
ErhI	CCWWGG	4 cut(s)	97, 2442, 2478, 2947
Esp3I	CGTCTC	1 cut(s)	40
FaqI	GGGAC	5 cut(s)	1395, 1964, 2564, 2801, 3155
FblI	GTMKAC	2 cut(s)	42, 872
Fnu4HI	GCNGC	8 cut(s)	59, 230, 337, 432, 476, 860, 966, 1359
FokI	GGATG	7 cut(s)	550, 561, 1628, 1640, 2495, 2615, 2723
FriOI	GRGCYC	2 cut(s)	1775, 2571
Fsp4HI	GCNGC	8 cut(s)	59, 230, 337, 432, 476, 860, 966, 1359
FspBI	CTAG	7 cut(s)	351, 627, 1325, 1728, 1851, 2214, 2787
FspI	TGCGCA	1 cut(s)	2035
GlaI	GCGC	6 cut(s)	57, 142, 211, 2035, 3021, 3160
GluI	GCNGC	8 cut(s)	59, 230, 337, 432, 476, 860, 966, 1359
GsuI	CTGGAG	2 cut(s)	1690, 1717
HaeII	RGCGCY	2 cut(s)	59, 144
HaeIII	GGCC	6 cut(s)	72, 687, 1358, 2334, 3093, 3165
HapII	CCGG	2 cut(s)	1421, 2600
HgaI	GACGC	1 cut(s)	2687
HhaI	GCGC	6 cut(s)	58, 143, 212, 2036, 3022, 3161
Hin1I	GRCGYC	2 cut(s)	56, 141
Hin6I	GCGC	6 cut(s)	56, 141, 210, 2034, 3020, 3159
HinP1I	GCGC	6 cut(s)	56, 141, 210, 2034, 3020, 3159
HincII	GTYRAC	4 cut(s)	43, 520, 2859, 3174
HindII	GTYRAC	4 cut(s)	43, 520, 2859, 3174
HindIII	AAGCTT	4 cut(s)	282, 356, 1046, 2431
HpaI	GTTAAC	1 cut(s)	2859
HpaII	CCGG	2 cut(s)	1421, 2600
HphI	GGTGA	9 cut(s)	121, 254, 696, 770, 1526, 1598, 2291, 2865, 2905
Hpy99I	CGWCG	2 cut(s)	44, 1294
HpyCH4IV	ACGT	4 cut(s)	682, 802, 1888, 2243
HpyF10VI	GCNNNNNNNGC	3 cut(s)	64, 149, 2430
HpySE526I	ACGT	4 cut(s)	682, 802, 1888, 2243
Hsp92I	GRCGYC	2 cut(s)	56, 141
HspAI	GCGC	6 cut(s)	56, 141, 210, 2034, 3020, 3159
KasI	GGCGCC	2 cut(s)	55, 140
KflI	GGGWCCC	1 cut(s)	3142
Kpn2I	TCCGGA	1 cut(s)	2599
KroI	GCCGGC	1 cut(s)	1420
KroNI	GCCGGC	1 cut(s)	1422
KspAI	GTTAAC	1 cut(s)	2859
Kzo9I	GATC	8 cut(s)	30, 778, 979, 1199, 1564, 1970, 3104, 3214
LguI	GCTCTTC	1 cut(s)	2695
LmnI	GCTCC	2 cut(s)	1120, 1736
Lsp1109I	GCAGC	6 cut(s)	216, 348, 418, 487, 871, 977
MabI	ACCWGGT	1 cut(s)	1608
MaeI	CTAG	7 cut(s)	351, 627, 1325, 1728, 1851, 2214, 2787
MaeII	ACGT	4 cut(s)	682, 802, 1888, 2243
MaeIII	GTNAC	3 cut(s)	365, 915, 1306
MalI	GATC	8 cut(s)	32, 780, 981, 1201, 1566, 1972, 3106, 3216
MboI	GATC	8 cut(s)	30, 778, 979, 1199, 1564, 1970, 3104, 3214
MflI	RGATCY	2 cut(s)	778, 1199
MhlI	GDGCHC	3 cut(s)	1062, 1775, 2571
MlsI	TGGCCA	1 cut(s)	687
MluNI	TGGCCA	1 cut(s)	687
Mly113I	GGCGCC	2 cut(s)	56, 141
MlyI	GAGTC	3 cut(s)	8, 2102, 2546
MmeI	TCCRAC	1 cut(s)	1642
Mox20I	TGGCCA	1 cut(s)	687
MroI	TCCGGA	1 cut(s)	2599
MroNI	GCCGGC	1 cut(s)	1420
MroXI	GAANNNNTTC	1 cut(s)	1573
MscI	TGGCCA	1 cut(s)	687
MslI	CAYNNNNRTG	1 cut(s)	549
Msp20I	TGGCCA	1 cut(s)	687
MspA1I	CMGCKG	2 cut(s)	229, 1361
MspCI	CTTAAG	1 cut(s)	863
MspI	CCGG	2 cut(s)	1421, 2600
MspR9I	CCNGG	4 cut(s)	69, 347, 1610, 2178
Mva1269I	GAATGC	2 cut(s)	2370, 3068
MvaI	CCWGG	4 cut(s)	69, 347, 1610, 2178
MwoI	GCNNNNNNNGC	3 cut(s)	64, 149, 2430
NaeI	GCCGGC	1 cut(s)	1422
NarI	GGCGCC	2 cut(s)	56, 141
NcoI	CCATGG	1 cut(s)	2478
NdeII	GATC	8 cut(s)	30, 778, 979, 1199, 1564, 1970, 3104, 3214
NgoMIV	GCCGGC	1 cut(s)	1420
NheI	GCTAGC	1 cut(s)	1727
NlaIV	GGNNCC	9 cut(s)	57, 120, 142, 772, 1162, 1848, 2990, 3143, 3144
NmuCI	GTSAC	1 cut(s)	915
NsbI	TGCGCA	1 cut(s)	2035
NspI	RCATGY	5 cut(s)	326, 554, 658, 1823, 2237
PagI	TCATGA	5 cut(s)	415, 481, 2794, 2965, 2974
PaqCI	CACCTGC	1 cut(s)	2872
PceI	AGGCCT	1 cut(s)	72
PciI	ACATGT	2 cut(s)	322, 550
PciSI	GCTCTTC	1 cut(s)	2695
PcsI	WCGNNNNNNNCGW	1 cut(s)	39
PctI	GAATGC	2 cut(s)	2370, 3068
PdiI	GCCGGC	1 cut(s)	1422
PdmI	GAANNNNTTC	1 cut(s)	1573
Pfl23II	CGTACG	1 cut(s)	25
PflFI	GACNNNGTC	1 cut(s)	2374
PkrI	GCNGC	8 cut(s)	60, 231, 338, 433, 477, 861, 967, 1360
PleI	GAGTC	3 cut(s)	8, 2101, 2546
PluTI	GGCGCC	2 cut(s)	59, 144
PpsI	GAGTC	3 cut(s)	8, 2101, 2546
Ppu21I	YACGTR	1 cut(s)	683
PpuMI	RGGWCCY	4 cut(s)	1701, 1846, 2951, 3142
PscI	ACATGT	2 cut(s)	322, 550
PsiI	TTATAA	3 cut(s)	128, 2142, 2151
Psp5II	RGGWCCY	4 cut(s)	1701, 1846, 2951, 3142
Psp6I	CCWGG	4 cut(s)	67, 345, 1608, 2176
PspEI	GGTNACC	1 cut(s)	365
PspGI	CCWGG	4 cut(s)	67, 345, 1608, 2176
PspLI	CGTACG	1 cut(s)	25
PspN4I	GGNNCC	9 cut(s)	57, 120, 142, 772, 1162, 1848, 2990, 3143, 3144
PspPI	GGNCC	7 cut(s)	1160, 1701, 1846, 2333, 2951, 3092, 3142
PspPPI	RGGWCCY	4 cut(s)	1701, 1846, 2951, 3142
PsrI	GAACNNNNNNTAC	2 cut(s)	292, 324
PstNI	CAGNNNCTG	2 cut(s)	1898, 2066
PsuI	RGATCY	2 cut(s)	778, 1199
PsyI	GACNNNGTC	1 cut(s)	2374
PvuII	CAGCTG	1 cut(s)	229
RsaI	GTAC	7 cut(s)	27, 194, 251, 327, 1858, 2065, 2242
RsaNI	GTAC	7 cut(s)	26, 193, 250, 326, 1857, 2064, 2241
RseI	CAYNNNNRTG	1 cut(s)	549
SalI	GTCGAC	1 cut(s)	41
SapI	GCTCTTC	1 cut(s)	2695
SatI	GCNGC	8 cut(s)	59, 230, 337, 432, 476, 860, 966, 1359
Sau3AI	GATC	8 cut(s)	30, 778, 979, 1199, 1564, 1970, 3104, 3214
Sau96I	GGNCC	7 cut(s)	1160, 1701, 1846, 2333, 2951, 3092, 3142
SchI	GAGTC	3 cut(s)	8, 2102, 2546
ScrFI	CCNGG	4 cut(s)	69, 347, 1610, 2178
SduI	GDGCHC	3 cut(s)	1062, 1775, 2571
SexAI	ACCWGGT	1 cut(s)	1608
SfcI	CTRYAG	2 cut(s)	273, 837
SfoI	GGCGCC	2 cut(s)	57, 142
SinI	GGWCC	5 cut(s)	1160, 1701, 1846, 2951, 3142
SmiMI	CAYNNNNRTG	1 cut(s)	549
SmlI	CTYRAG	4 cut(s)	821, 863, 2340, 2955
SmoI	CTYRAG	4 cut(s)	821, 863, 2340, 2955
SseBI	AGGCCT	1 cut(s)	72
SsiI	CCGC	2 cut(s)	59, 1359
SspDI	GGCGCC	2 cut(s)	55, 140
SspI	AATATT	1 cut(s)	135
SspMI	CTAG	7 cut(s)	351, 627, 1325, 1728, 1851, 2214, 2787
StuI	AGGCCT	1 cut(s)	72
StyD4I	CCNGG	4 cut(s)	67, 345, 1608, 2176
StyI	CCWWGG	4 cut(s)	97, 2442, 2478, 2947
TaiI	ACGT	4 cut(s)	685, 805, 1891, 2246
TaqI	TCGA	7 cut(s)	42, 75, 1087, 1135, 1567, 2053, 3221
TatI	WGTACW	1 cut(s)	325
TauI	GCSGC	2 cut(s)	61, 1361
TscAI	CASTG	5 cut(s)	1069, 1903, 2211, 2509, 3007
TseFI	GTSAC	1 cut(s)	915
TseI	GCWGC	6 cut(s)	229, 336, 431, 475, 859, 965
Tsp45I	GTSAC	1 cut(s)	915
TspGWI	ACGGA	5 cut(s)	28, 43, 560, 633, 2723
TspRI	CASTG	5 cut(s)	1069, 1903, 2211, 2509, 3007
Tth111I	GACNNNGTC	1 cut(s)	2374
Vha464I	CTTAAG	1 cut(s)	863
VneI	GTGCAC	1 cut(s)	1058
VpaK11BI	GGWCC	5 cut(s)	1160, 1701, 1846, 2951, 3142
XagI	CCTNNNNNAGG	1 cut(s)	344
XapI	RAATTY	7 cut(s)	566, 748, 761, 1034, 1573, 1694, 2757
XceI	RCATGY	5 cut(s)	326, 554, 658, 1823, 2237
XmiI	GTMKAC	2 cut(s)	42, 872
XmnI	GAANNNNTTC	1 cut(s)	1573
XspI	CTAG	7 cut(s)	351, 627, 1325, 1728, 1851, 2214, 2787

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.