pycom12g20620

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004748
Physical Location & Seq
Reverse (-)
22057119 .. 22062681
5563 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3141 bp
ATGATCTTTTTCCAAACAAATCGAGCAACAACGAGATCTGAACCAGCGCAAAACGGAACATCCCTCCATAGACGCACAGAAGCACAGAGAGAGGCCCACACACAGAGCTTCAAAGCCCTCATAGCCATGGCGGAGGTCGACGACGCCGGGGGCTTCGGAGACTTCAAGTTCTTCACCGCCGTCGATCCCAAGCCCAAGATCAACGGACGAGATTCGACCGTCTTGGACGACGATTGGGGCGACTTCGTAACGGACAGCACGAGTCAGATCAAAGCTCAGGCCGTGCCTTCCAATGGAATCACGTACTCCCAGTCACCTCCCTCCCAAATACCCGTCGACCCTTTGGGGTTTTGCGTTCCTAATGGGTCGGCGCCGACCCGAACGGAATCAGAGCCAATCCGAGTTGAGACTGAGCCCGCGAAGGTTGATAAGACTCGGTGGGTAAAGCCGCAGGGAGCTTTGCCGTTATCGTTATTTGGGGAAGAGCAGGAAGAGGACTCCGGTGCGGGCGAGTCCAAGGTTGGGGACGTTGCAAACGAAGGATTTGCGAAAAAGGAGTCGATTTTGAATGTGAGAAGTGTTGGGATCGATGATTTGATTTCAAACTTGTACGGTCATAATCCAAAAGTCGTGGTTCGAAATGGGTCGGATTCGAATTTCGGTCCCGGAGGTCCCAATTCAACAAAAAAGGGGTTGGATTTCAGTGCAAATGGGATGGATTTGAAGTTTGATGCTCCCATTCCGAACGGAAATGGGAAATTTGGTGGTTTGAATTTTGGTTCGAATAGTTTTGATTTGAAGTTTGATGATGTGGGTTCGAATTCAAATGCAAGTGTGTCAAATTCGGGTTCTGGTGGTCCTCATACGGTAAAAAACGGATTGAATTTCAGTGCAGATGCATTGGATTTGAAGCTTGATGCTCTGGTTCCGAAAGAAAATGGGACATTGGGTGGCTTGAATTTGGATTCGAATCGTTTTGATTTGAAATTTGATGGAGTAGATTCGAATTCAAATACACATGGGTTGAAGTTGAATTTGGTGGAAGGAAATGGCGACTTGGATGAGGAGGATGATGATGGTTGGGAATTCAAAGCTGCGGATTTGAAGGAACAAGGGGTTGTGGAAGTAGCAGGATCGAAAGCAGAATCAAATGCACACGCACGACCAGGAATCCAGGTTGATGAGAAGTGGCAAGCAAATACTGGAGGAAGTGGATTTAGTGAAGGGTTTTCATTTGATTTCAACCCAAAGCCTGTTACTCAGCATAATTTTTTCTTCGACCCACTTTCGAAAAGCAAGCAGAACAGTGCTGCAGATATACCCAATTCTACTCCAGTTAATGGAAATCTGTGGGAGTTAAAGGGTGCATTTTCAGAAACTGGAGCAAAACATAAGTTGGAAGAGGCAAAGGCTGCCAATCCTGCTGGTTTTGGCGCCCGTGCTCATAATGATTTTTTTGCTACATTTCAAGGGGATTCCCACAAGTCTGGAGAAAACTTTGCATTTCCTTTTATACCGACTTCTAGCATGAAACATGGTGTAATCTCAGATTCACATTCCAGTGGCAAGAAAGAGGACATTGAAAAAGAGTTGAGTAGTTCTCCTGATGTTAGGTCTGATGACAATTTTTGGGAATTGAACAATGCATTTTCCGAATCTGGATCAAAGATTGAGGGGGAGTTAGTGGTTGGCGGTAATCCTCCTGCCAATATAAAACCATCTGTCATGGGTTTTGAAATCCAGCATAATGAGGTAACATTGGAAAATCATCGACGAGCCTTGCCCTTGTCCATTTTTGGAGATGATGAACTAGAGACTGATGATTCCTCAATTCAACAAAATATTTCTTCTCTTACCACTGCATCCCACCAAGTAAATACCAACAAAAGCCCTGCGTCCAATCTATCCATCACAGATCTAATATCAAGCTTATACAGTCAAGTGGACCAGAACACTAACACAATCCATGCGCCGAAACCAACTGAAAATATTATGGATCCTGCCCCAACCTTTTTGGAATCCAATTTTGGTGGTGATGATTTTGATGAAGATTCTTGGGAATTTAAGGATGCTGTTACAAGTGATCAAAACCAAACCTCTATTGCTACTCTTGAAGATTCACCTCACGATTCTTCTACTAAAGTCCATCTAGATAATTATGTGGATTTATATAGCAAATTGAAGGATGAAACATACGCTCTTGCACTCTACCATCTTGAAAATAAAAAGAAAGCTCAAAGTGGTGCTACTCTTTCTGGTGAAGATACAACAATAAAGACTATGGAAGAGGAAATCCAGAAATTGTACAATGAACTGCATCAACACAATATGATCTCCGATCAATTCCAGTCTGGGAGTCTTTCATCAAGAAATACACACATTCAAGAAGTTCGTAAACTTCTACAGGATCCAAAGTTCCAAGTTCTTGAATCCGAATACCAGTTGTCACAAAGGTTGTCATTGGCAGAGAATGATTTGAGATCTGCCGTTGAACTTTCAAGACATGCAGCATCCACGTTAAGGATTCTTAGATTGGGATCAACTGAGCAACAATCTAACTACATTTCCACCTGGTCTCGAATAGTCTCCTTTTGCGCTGAAGAGTTGAAACATGGTTCTCTAATTTGGAGGCAATCATTAGAAGCGAATGTTCAGAATCAAATACTATCTGAACCTCAAGGCAAGCAGTATATCGTTGCCCTTGGAGAAATTTACAGAGTAGTTTTAGTTCTTGAAGCATCTGCCAAACTTTACGAGTCATGGATTCTATTACATTCTTCAAACTGTTTTACCTTTTTTTCTCTTCTAAACGAGTGCTCTACTTTATGGTCAAGTTCGGGACTTGATGAAGCTCTCAAGAGCATTTCGGATGCGATTGATTTTAAATATGATGAGACCGTTGCTGCACTACTAGATTCCATGACATACATTCATCATCTGGATGCATTTGCTCTCCAAAACCAAGTTGTTGTTAATGGCCAAGAACCTATTTGCTCGCTGTCACTATTAACTGCAGGAACCGTGCCAGGTATTAAAATGGTGATGTGGAAAGGGGAAAACTACTTATTGACGCTTGCGAACTTGTGGGCAAATTTAATTAGTCCTAATCCTCCAGAATTGCCCCACTTGAGTTATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1047

Amino Acids

114.21

Weight (kDa)

4.79

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYNRG_C PF25999 847 - 1040 5.6e-64 Synergin gamma, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 370, 1433
AccB7I CCANNNNNTGG 1 cut(s) 1340
AccI GTMKAC 2 cut(s) 138, 336
AccII CGCG 1 cut(s) 419
AciI CCGC 7 cut(s) 131, 177, 417, 449, 506, 1097, 1692
AclWI GGATC 9 cut(s) 179, 593, 1141, 1669, 1991, 2004, 2402, 2415, 2545
AcoI YGGCCR 1 cut(s) 2977
AcuI CTGAAG 1 cut(s) 2619
AcyI GRCGYC 3 cut(s) 144, 371, 1434
AfaI GTAC 3 cut(s) 305, 611, 2306
AfiI CCNNNNNNNGG 4 cut(s) 293, 522, 1340, 2520
AjnI CCWGG 4 cut(s) 1165, 1173, 2570, 3025
AjuI GAANNNNNNNTTGG 4 cut(s) 1019, 1051, 2010, 2042
AloI GAACNNNNNNTCC 2 cut(s) 2399, 2431
AluBI AGCT 9 cut(s) 108, 275, 458, 913, 1094, 1929, 2234, 2852, 3138
AluI AGCT 9 cut(s) 108, 275, 458, 913, 1094, 1929, 2234, 2852, 3138
Alw21I GWGCWC 2 cut(s) 1444, 2819
Alw26I GTCTC 6 cut(s) 153, 401, 1808, 2580, 2590, 2888
AlwI GGATC 9 cut(s) 179, 593, 1141, 1669, 1991, 2004, 2402, 2415, 2545
AlwNI CAGNNNCTG 1 cut(s) 1379
AoxI GGCC 3 cut(s) 93, 279, 2977
ApeKI GCWGC 5 cut(s) 1094, 1310, 1412, 2507, 2903
ArsI GACNNNNNNTTYG 2 cut(s) 542, 574
AspLEI GCGC 5 cut(s) 49, 373, 1436, 1972, 2597
AspS9I GGNCC 5 cut(s) 94, 662, 671, 857, 1945
AsuC2I CCSGG 2 cut(s) 148, 666
AsuHPI GGTGA 6 cut(s) 166, 306, 2045, 2112, 2270, 3052
AsuII TTCGAA 7 cut(s) 637, 653, 782, 818, 968, 1004, 1289
AvaII GGWCC 4 cut(s) 662, 671, 857, 1945
BalI TGGCCA 1 cut(s) 2979
BamHI GGATCC 2 cut(s) 1996, 2407
BanI GGYRCC 2 cut(s) 370, 1433
BanII GRGCYC 1 cut(s) 417
BauI CACGAG 1 cut(s) 259
Bbv12I GWGCWC 2 cut(s) 1444, 2819
BbvI GCAGC 5 cut(s) 1081, 1297, 1399, 2519, 2890
BccI CCATC 7 cut(s) 709, 986, 1070, 1726, 1916, 2154, 2220
BceAI ACGGC 4 cut(s) 164, 266, 448, 2471
BcgI CGANNNNNNTGC 2 cut(s) 527, 561
BciT130I CCWGG 4 cut(s) 1167, 1175, 2572, 3027
BclI TGATCA 1 cut(s) 2083
BcnI CCSGG 2 cut(s) 148, 666
BcoDI GTCTC 6 cut(s) 153, 401, 1808, 2580, 2590, 2888
BfaI CTAG 4 cut(s) 1524, 1811, 2150, 2912
BfmI CTRYAG 3 cut(s) 1311, 2402, 3012
BfoI RGCGCY 2 cut(s) 374, 1437
BglII AGATCT 3 cut(s) 35, 1915, 2480
BisI GCNGC 6 cut(s) 449, 1095, 1311, 1413, 2508, 2904
BlsI GCNGC 6 cut(s) 450, 1096, 1312, 1414, 2509, 2905
Bme1390I CCNGG 6 cut(s) 148, 666, 1167, 1175, 2572, 3027
Bme18I GGWCC 4 cut(s) 662, 671, 857, 1945
BmgT120I GGNCC 5 cut(s) 94, 662, 671, 857, 1945
BmiI GGNNCC 8 cut(s) 372, 664, 673, 927, 1435, 1998, 2409, 3019
BmrFI CCNGG 6 cut(s) 148, 666, 1167, 1175, 2572, 3027
BmrI ACTGGG 1 cut(s) 304
BmuI ACTGGG 1 cut(s) 304
BplI GAGNNNNNCTC 2 cut(s) 1585, 1617
BpmI CTGGAG 5 cut(s) 1224, 1317, 1401, 1509, 3096
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 7 cut(s) 637, 653, 782, 818, 968, 1004, 1289
BpuEI CTTGAG 2 cut(s) 2661, 2840
BpuMI CCSGG 2 cut(s) 148, 666
Bsa29I ATCGAT 1 cut(s) 588
BsaAI YACGTR 1 cut(s) 303
BsaBI GATNNNNATC 2 cut(s) 969, 2536
BsaHI GRCGYC 3 cut(s) 144, 371, 1434
BsaI GGTCTC 2 cut(s) 2580, 2888
BsaJI CCNNGG 4 cut(s) 126, 147, 516, 2701
BsaWI WCCGGW 1 cut(s) 500
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 522, 1340, 2520
Bse1I ACTGG 7 cut(s) 310, 1207, 1334, 1384, 1560, 2347, 2440
Bse8I GATNNNNATC 2 cut(s) 969, 2536
BseBI CCWGG 4 cut(s) 1167, 1175, 2572, 3027
BseCI ATCGAT 1 cut(s) 588
BseDI CCNNGG 4 cut(s) 126, 147, 516, 2701
BseJI GATNNNNATC 2 cut(s) 969, 2536
BseLI CCNNNNNNNGG 4 cut(s) 293, 522, 1340, 2520
BseMII CTCAG 5 cut(s) 290, 402, 1274, 1560, 2535
BseNI ACTGG 7 cut(s) 310, 1207, 1334, 1384, 1560, 2347, 2440
BseRI GAGGAG 1 cut(s) 1079
BseXI GCAGC 5 cut(s) 1081, 1297, 1399, 2519, 2890
BsgI GTGCAG 2 cut(s) 912, 2889
Bsh1236I CGCG 1 cut(s) 419
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 3 cut(s) 95, 281, 2979
BshNI GGYRCC 2 cut(s) 370, 1433
BshVI ATCGAT 1 cut(s) 588
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 2 cut(s) 1444, 2819
BsiSI CCGG 3 cut(s) 147, 501, 666
BslFI GGGAC 5 cut(s) 539, 648, 657, 955, 2853
BslI CCNNNNNNNGG 4 cut(s) 293, 522, 1340, 2520
BsmAI GTCTC 6 cut(s) 153, 401, 1808, 2580, 2590, 2888
BsmFI GGGAC 5 cut(s) 539, 648, 657, 955, 2853
BsnI GGCC 3 cut(s) 95, 281, 2979
Bso31I GGTCTC 2 cut(s) 2580, 2888
Bsp119I TTCGAA 7 cut(s) 637, 653, 782, 818, 968, 1004, 1289
Bsp1286I GDGCHC 3 cut(s) 417, 1444, 2819
Bsp1407I TGTACA 1 cut(s) 2304
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 7 cut(s) 131, 177, 417, 449, 506, 1097, 1692
BspANI GGCC 3 cut(s) 95, 281, 2979
BspCNI CTCAG 5 cut(s) 289, 403, 1273, 1559, 2536
BspDI ATCGAT 1 cut(s) 588
BspFNI CGCG 1 cut(s) 419
BspLI GGNNCC 8 cut(s) 372, 664, 673, 927, 1435, 1998, 2409, 3019
BspMAI CTGCAG 2 cut(s) 1315, 3016
BspPI GGATC 9 cut(s) 179, 593, 1141, 1669, 1991, 2004, 2402, 2415, 2545
BspQI GCTCTTC 1 cut(s) 477
BspT104I TTCGAA 7 cut(s) 637, 653, 782, 818, 968, 1004, 1289
BspT107I GGYRCC 2 cut(s) 370, 1433
BspTNI GGTCTC 2 cut(s) 2580, 2888
BsrGI TGTACA 1 cut(s) 2304
BsrI ACTGG 7 cut(s) 310, 1207, 1334, 1384, 1560, 2347, 2440
BssECI CCNNGG 4 cut(s) 126, 147, 516, 2701
BssNI GRCGYC 3 cut(s) 144, 371, 1434
BssSI CACGAG 1 cut(s) 259
BssT1I CCWWGG 3 cut(s) 126, 516, 2701
Bst2BI CACGAG 1 cut(s) 259
Bst2UI CCWGG 4 cut(s) 1167, 1175, 2572, 3027
Bst4CI ACNGT 8 cut(s) 220, 614, 868, 1307, 1937, 2786, 2899, 3022
Bst6I CTCTTC 6 cut(s) 477, 486, 1395, 2280, 2595, 2808
BstACI GRCGYC 3 cut(s) 144, 371, 1434
BstAPI GCANNNNNTGC 1 cut(s) 1412
BstAUI TGTACA 1 cut(s) 2304
BstBAI YACGTR 1 cut(s) 303
BstBI TTCGAA 7 cut(s) 637, 653, 782, 818, 968, 1004, 1289
BstC8I GCNNGC 7 cut(s) 417, 508, 1194, 1298, 2684, 2996, 3075
BstDEI CTNAG 6 cut(s) 276, 411, 1260, 1546, 2528, 2544
BstDSI CCRYGG 1 cut(s) 126
BstFNI CGCG 1 cut(s) 419
BstH2I RGCGCY 2 cut(s) 374, 1437
BstHHI GCGC 5 cut(s) 49, 373, 1436, 1972, 2597
BstMAI GTCTC 6 cut(s) 153, 401, 1808, 2580, 2590, 2888
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 3 cut(s) 122, 1412, 1421
BstNI CCWGG 4 cut(s) 1167, 1175, 2572, 3027
BstNSI RCATGY 1 cut(s) 2507
BstSCI CCNGG 6 cut(s) 146, 664, 1165, 1173, 2570, 3025
BstSFI CTRYAG 3 cut(s) 1311, 2402, 3012
BstUI CGCG 1 cut(s) 419
BstV1I GCAGC 5 cut(s) 1081, 1297, 1399, 2519, 2890
BstX2I RGATCY 5 cut(s) 35, 1915, 1996, 2407, 2480
BstXI CCANNNNNNTGG 1 cut(s) 1487
BstYI RGATCY 5 cut(s) 35, 1915, 1996, 2407, 2480
Bsu15I ATCGAT 1 cut(s) 588
BsuRI GGCC 3 cut(s) 95, 281, 2979
BsuTUI ATCGAT 1 cut(s) 588
BtgI CCRYGG 1 cut(s) 126
BtsI GCAGTG 1 cut(s) 1857
BtsIMutI CAGTG 5 cut(s) 709, 895, 1312, 1567, 1857
Cac8I GCNNGC 7 cut(s) 417, 508, 1194, 1298, 2684, 2996, 3075
CaiI CAGNNNCTG 1 cut(s) 1379
CfoI GCGC 5 cut(s) 49, 373, 1436, 1972, 2597
Cfr13I GGNCC 5 cut(s) 94, 662, 671, 857, 1945
ClaI ATCGAT 1 cut(s) 588
CseI GACGC 4 cut(s) 81, 152, 1884, 3079
CsiI ACCWGGT 1 cut(s) 2570
Csp6I GTAC 3 cut(s) 304, 610, 2305
CspCI CAANNNNNGTGG 4 cut(s) 612, 647, 2011, 2046
CviQI GTAC 3 cut(s) 304, 610, 2305
DdeI CTNAG 6 cut(s) 276, 411, 1260, 1546, 2528, 2544
DinI GGCGCC 2 cut(s) 372, 1435
DraI TTTAAA 1 cut(s) 2884
EaeI YGGCCR 1 cut(s) 2977
Eam1104I CTCTTC 6 cut(s) 477, 486, 1395, 2280, 2595, 2808
EarI CTCTTC 6 cut(s) 477, 486, 1395, 2280, 2595, 2808
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 3 cut(s) 126, 516, 2701
Eco24I GRGCYC 1 cut(s) 417
Eco31I GGTCTC 2 cut(s) 2580, 2888
Eco47I GGWCC 4 cut(s) 662, 671, 857, 1945
Eco57I CTGAAG 1 cut(s) 2619
EcoO109I RGGNCCY 1 cut(s) 671
EcoRI GAATTC 3 cut(s) 820, 1006, 1085
EcoRII CCWGG 4 cut(s) 1165, 1173, 2570, 3025
EcoT14I CCWWGG 3 cut(s) 126, 516, 2701
EcoT22I ATGCAT 3 cut(s) 901, 1648, 2947
EcoT38I GRGCYC 1 cut(s) 417
EgeI GGCGCC 2 cut(s) 372, 1435
EheI GGCGCC 2 cut(s) 372, 1435
ErhI CCWWGG 3 cut(s) 126, 516, 2701
FaqI GGGAC 5 cut(s) 539, 648, 657, 955, 2853
FauI CCCGC 2 cut(s) 424, 499
FbaI TGATCA 1 cut(s) 2083
FblI GTMKAC 2 cut(s) 138, 336
Fnu4HI GCNGC 6 cut(s) 449, 1095, 1311, 1413, 2508, 2904
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 6 cut(s) 449, 1095, 1311, 1413, 2508, 2904
FspBI CTAG 4 cut(s) 1524, 1811, 2150, 2912
GlaI GCGC 5 cut(s) 48, 372, 1435, 1971, 2596
GluI GCNGC 6 cut(s) 449, 1095, 1311, 1413, 2508, 2904
GsuI CTGGAG 5 cut(s) 1224, 1317, 1401, 1509, 3096
HaeII RGCGCY 2 cut(s) 374, 1437
HaeIII GGCC 3 cut(s) 95, 281, 2979
HapII CCGG 3 cut(s) 147, 501, 666
HgaI GACGC 4 cut(s) 81, 152, 1884, 3079
HhaI GCGC 5 cut(s) 49, 373, 1436, 1972, 2597
Hin1I GRCGYC 3 cut(s) 144, 371, 1434
Hin6I GCGC 5 cut(s) 47, 371, 1434, 1970, 2595
HinP1I GCGC 5 cut(s) 47, 371, 1434, 1970, 2595
HincII GTYRAC 2 cut(s) 139, 337
HindII GTYRAC 2 cut(s) 139, 337
HindIII AAGCTT 2 cut(s) 911, 1927
HpaII CCGG 3 cut(s) 147, 501, 666
HphI GGTGA 6 cut(s) 166, 306, 2045, 2112, 2270, 3052
Hpy166II GTNNAC 4 cut(s) 139, 337, 1945, 2396
Hpy8I GTNNAC 4 cut(s) 139, 337, 1945, 2396
Hpy99I CGWCG 6 cut(s) 143, 146, 185, 233, 338, 1776
HpyAV CCTTC 7 cut(s) 297, 415, 533, 1037, 1099, 1217, 2176
HpyCH4III ACNGT 8 cut(s) 220, 614, 868, 1307, 1937, 2786, 2899, 3022
HpyCH4IV ACGT 3 cut(s) 302, 528, 2516
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 1412, 1421
HpyF3I CTNAG 6 cut(s) 276, 411, 1260, 1546, 2528, 2544
HpySE526I ACGT 3 cut(s) 302, 528, 2516
Hsp92I GRCGYC 3 cut(s) 144, 371, 1434
HspAI GCGC 5 cut(s) 47, 371, 1434, 1970, 2595
KasI GGCGCC 2 cut(s) 370, 1433
Ksp22I TGATCA 1 cut(s) 2083
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 3 cut(s) 455, 739, 1382
Lsp1109I GCAGC 5 cut(s) 1081, 1297, 1399, 2519, 2890
MabI ACCWGGT 1 cut(s) 2570
MaeI CTAG 4 cut(s) 1524, 1811, 2150, 2912
MaeII ACGT 3 cut(s) 302, 528, 2516
MaeIII GTNAC 7 cut(s) 247, 312, 1255, 1753, 2074, 2445, 3000
MflI RGATCY 5 cut(s) 35, 1915, 1996, 2407, 2480
MhlI GDGCHC 3 cut(s) 417, 1444, 2819
MlsI TGGCCA 1 cut(s) 2979
MluNI TGGCCA 1 cut(s) 2979
Mly113I GGCGCC 2 cut(s) 371, 1434
MlyI GAGTC 7 cut(s) 271, 427, 491, 521, 566, 2365, 2765
MmeI TCCRAC 3 cut(s) 627, 675, 1377
Mox20I TGGCCA 1 cut(s) 2979
Mph1103I ATGCAT 3 cut(s) 901, 1648, 2947
MscI TGGCCA 1 cut(s) 2979
MseI TTAA 9 cut(s) 1338, 1358, 2064, 2519, 2883, 2973, 3008, 3033, 3095
MslI CAYNNNNRTG 3 cut(s) 125, 1560, 2940
Msp20I TGGCCA 1 cut(s) 2979
MspI CCGG 3 cut(s) 147, 501, 666
MspR9I CCNGG 6 cut(s) 148, 666, 1167, 1175, 2572, 3027
MvaI CCWGG 4 cut(s) 1167, 1175, 2572, 3027
MvnI CGCG 1 cut(s) 419
MwoI GCNNNNNNNGC 3 cut(s) 122, 1412, 1421
NarI GGCGCC 2 cut(s) 371, 1434
NciI CCSGG 2 cut(s) 148, 666
NcoI CCATGG 1 cut(s) 126
NlaIV GGNNCC 8 cut(s) 372, 664, 673, 927, 1435, 1998, 2409, 3019
NmuCI GTSAC 3 cut(s) 312, 2445, 3000
NsiI ATGCAT 3 cut(s) 901, 1648, 2947
NspI RCATGY 1 cut(s) 2507
NspV TTCGAA 7 cut(s) 637, 653, 782, 818, 968, 1004, 1289
PciSI GCTCTTC 1 cut(s) 477
PcsI WCGNNNNNNNCGW 3 cut(s) 237, 257, 534
PflMI CCANNNNNTGG 1 cut(s) 1340
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 6 cut(s) 450, 1096, 1312, 1414, 2509, 2905
PleI GAGTC 7 cut(s) 270, 427, 491, 520, 565, 2364, 2764
PluTI GGCGCC 2 cut(s) 374, 1437
PpsI GAGTC 7 cut(s) 270, 427, 491, 520, 565, 2364, 2764
Ppu21I YACGTR 1 cut(s) 303
PpuMI RGGWCCY 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 671
Psp6I CCWGG 4 cut(s) 1165, 1173, 2570, 3025
PspGI CCWGG 4 cut(s) 1165, 1173, 2570, 3025
PspN4I GGNNCC 8 cut(s) 372, 664, 673, 927, 1435, 1998, 2409, 3019
PspPI GGNCC 5 cut(s) 94, 662, 671, 857, 1945
PspPPI RGGWCCY 1 cut(s) 671
PsrI GAACNNNNNNTAC 2 cut(s) 2712, 2744
PstI CTGCAG 2 cut(s) 1315, 3016
PstNI CAGNNNCTG 1 cut(s) 1379
PsuI RGATCY 5 cut(s) 35, 1915, 1996, 2407, 2480
RsaI GTAC 3 cut(s) 305, 611, 2306
RsaNI GTAC 3 cut(s) 304, 610, 2305
RseI CAYNNNNRTG 3 cut(s) 125, 1560, 2940
SalI GTCGAC 2 cut(s) 137, 335
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 9 cut(s) 1338, 1358, 2064, 2519, 2883, 2973, 3008, 3033, 3095
SatI GCNGC 6 cut(s) 449, 1095, 1311, 1413, 2508, 2904
Sau96I GGNCC 5 cut(s) 94, 662, 671, 857, 1945
SchI GAGTC 7 cut(s) 271, 427, 491, 521, 566, 2365, 2765
ScrFI CCNGG 6 cut(s) 148, 666, 1167, 1175, 2572, 3027
SduI GDGCHC 3 cut(s) 417, 1444, 2819
SexAI ACCWGGT 1 cut(s) 2570
SfcI CTRYAG 3 cut(s) 1311, 2402, 3012
SfoI GGCGCC 2 cut(s) 372, 1435
SfuI TTCGAA 7 cut(s) 637, 653, 782, 818, 968, 1004, 1289
SinI GGWCC 4 cut(s) 662, 671, 857, 1945
SmiMI CAYNNNNRTG 3 cut(s) 125, 1560, 2940
SmlI CTYRAG 3 cut(s) 2676, 2855, 3127
SmoI CTYRAG 3 cut(s) 2676, 2855, 3127
SsiI CCGC 7 cut(s) 131, 177, 417, 449, 506, 1097, 1692
SspDI GGCGCC 2 cut(s) 370, 1433
SspI AATATT 2 cut(s) 1843, 1990
SspMI CTAG 4 cut(s) 1524, 1811, 2150, 2912
StyD4I CCNGG 6 cut(s) 146, 664, 1165, 1173, 2570, 3025
StyI CCWWGG 3 cut(s) 126, 516, 2701
TaaI ACNGT 8 cut(s) 220, 614, 868, 1307, 1937, 2786, 2899, 3022
TaiI ACGT 3 cut(s) 305, 531, 2519
TaqII GACCGA 1 cut(s) 650
TatI WGTACW 1 cut(s) 2304
TauI GCSGC 1 cut(s) 451
Tru1I TTAA 9 cut(s) 1338, 1358, 2064, 2519, 2883, 2973, 3008, 3033, 3095
Tru9I TTAA 9 cut(s) 1338, 1358, 2064, 2519, 2883, 2973, 3008, 3033, 3095
TscAI CASTG 5 cut(s) 709, 895, 1312, 1567, 1864
TseFI GTSAC 3 cut(s) 312, 2445, 3000
TseI GCWGC 5 cut(s) 1094, 1310, 1412, 2507, 2903
Tsp45I GTSAC 3 cut(s) 312, 2445, 3000
TspDTI ATGAA 9 cut(s) 1221, 1544, 1821, 2061, 2202, 2325, 2352, 2862, 2921
TspGWI ACGGA 6 cut(s) 69, 219, 266, 398, 762, 891
TspRI CASTG 5 cut(s) 709, 895, 1312, 1567, 1864
Van91I CCANNNNNTGG 1 cut(s) 1340
VpaK11BI GGWCC 4 cut(s) 662, 671, 857, 1945
XbaI TCTAGA 1 cut(s) 2149
XceI RCATGY 1 cut(s) 2507
XmiI GTMKAC 2 cut(s) 138, 336
XspI CTAG 4 cut(s) 1524, 1811, 2150, 2912
Zsp2I ATGCAT 3 cut(s) 901, 1648, 2947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.