pycom12g23900

Basic leucine zipper and W2 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0006554
Physical Location & Seq
Forward (+)
24336844 .. 24339381
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 903 bp
ATGGCTGATAAATTAGTTTGTACAATGACCAAACCCGCATCACCCCTCTTCCCATACCTCTTTCCCCCTCCCTCTCGCCTCTCTCTTCGAAAAAAGCTCTCGACGCTCATAGACCTCACTGCCCTCCGATCCATCTTCGTCTTGGTTTATGAGTTCTTGTATTTCTGTTTCTTTTTCGTTTTGATCCAGTTTCTTGTGGTCTCGAAGGAGAGACCCACTCTCGGTGGCACGCGGATTAAGACCCGCAAACGGAATATTGCAGCGCCGCTGGACCCTGCAGCTTTTGCGGATGCAGTGGTCCAGATATATCTGGATAATGCTGGTGATCTGGAACTTATTGCCAAGAGCATTGAATCTTCAGACCTTAACTTCTCAAGATACGGTGACACCTTTTTTGAGGTTGTTTTCACTGGAGGCCGTACACAACCTGGAACAACAAAACCTGATGAGGGGGAGCGCCACCCTTACTCTGTATTAGAGTCTGAGCCTACGCGTGAACTCATCTTGCCATCGGTTATATACCTTCAAAAAATTTTGAGGCGGAGGCCATTTCTGATTAAGAATCTTGAAAATGTTATGCGACGATTCCTTCAATCGTTGGAGCTTTTTGAGGAAAATGAAAGGAAGAAGCTGGCTATTTTCACAGCACTTGCATTCTCTCAGAAGCTGTCAGGACTTCCACCGGAAACGGTGTTCCAGCCAATGCTCAAGGATAATCTTGTTGGCAAAGGGCTAGTTCTATCGTTCATTACTGAGTTTTTCAAGGAGTATTTGATTGATAATAGTCTTGATGATTTGATTTCCATTCTGAAGCGGGGTAAAGTGGAGGACAATCTTTTGGAATTCTTCCCATCCGCAAAGAGAACTGAAGAGAATTTCTCTGAGCATTTCACGAAGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
W2

Protein Analysis

301

Amino Acids

34.36

Weight (kDa)

8.69

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_5MP1_2 PF25504 79 - 299 9e-77 5MP1/2 HEAT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 232, 493
AciI CCGC 8 cut(s) 36, 232, 244, 266, 287, 541, 814, 855
AclWI GGATC 2 cut(s) 123, 178
AcsI RAATTY 3 cut(s) 531, 842, 874
AcuI CTGAAG 3 cut(s) 342, 830, 888
AfaI GTAC 2 cut(s) 22, 421
AfiI CCNNNNNNNGG 3 cut(s) 221, 249, 449
AflIII ACRYGT 1 cut(s) 491
AgsI TTSAA 5 cut(s) 353, 527, 569, 593, 763
AjnI CCWGG 1 cut(s) 427
AloI GAACNNNNNNTCC 2 cut(s) 677, 709
AluBI AGCT 5 cut(s) 97, 281, 604, 631, 667
AluI AGCT 5 cut(s) 97, 281, 604, 631, 667
Alw26I GTCTC 2 cut(s) 205, 205
AlwI GGATC 2 cut(s) 123, 178
AlwNI CAGNNNCTG 1 cut(s) 667
AoxI GGCC 2 cut(s) 415, 545
ApeKI GCWGC 2 cut(s) 260, 278
ApoI RAATTY 3 cut(s) 531, 842, 874
ArsI GACNNNNNNTTYG 2 cut(s) 377, 409
AspLEI GCGC 2 cut(s) 265, 459
AspS9I GGNCC 2 cut(s) 271, 298
AsuHPI GGTGA 3 cut(s) 33, 335, 395
AsuII TTCGAA 1 cut(s) 88
AvaII GGWCC 2 cut(s) 271, 298
BbvI GCAGC 2 cut(s) 272, 290
BccI CCATC 3 cut(s) 140, 517, 859
BceAI ACGGC 1 cut(s) 402
BciT130I CCWGG 1 cut(s) 429
BcoDI GTCTC 2 cut(s) 205, 205
BfaI CTAG 2 cut(s) 734, 901
BfmI CTRYAG 1 cut(s) 276
BfoI RGCGCY 2 cut(s) 266, 460
BisI GCNGC 3 cut(s) 261, 266, 279
BlsI GCNGC 3 cut(s) 262, 267, 280
Bme1390I CCNGG 1 cut(s) 429
Bme18I GGWCC 2 cut(s) 271, 298
BmgT120I GGNCC 2 cut(s) 271, 298
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 1 cut(s) 429
BmsI GCATC 2 cut(s) 47, 280
BplI GAGNNNNNCTC 4 cut(s) 202, 234, 863, 895
BpmI CTGGAG 1 cut(s) 432
Bpu14I TTCGAA 1 cut(s) 88
BpuEI CTTGAG 2 cut(s) 358, 692
BsaI GGTCTC 2 cut(s) 205, 205
BsaWI WCCGGW 1 cut(s) 682
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 249, 449
Bse1I ACTGG 2 cut(s) 187, 415
BseBI CCWGG 1 cut(s) 429
BseGI GGATG 2 cut(s) 295, 851
BseLI CCNNNNNNNGG 3 cut(s) 221, 249, 449
BseMII CTCAG 4 cut(s) 474, 674, 744, 873
BseNI ACTGG 2 cut(s) 187, 415
BseXI GCAGC 2 cut(s) 272, 290
Bsh1236I CGCG 2 cut(s) 232, 493
BshFI GGCC 2 cut(s) 417, 547
BsiSI CCGG 1 cut(s) 683
BslI CCNNNNNNNGG 3 cut(s) 221, 249, 449
BsmAI GTCTC 2 cut(s) 205, 205
BsmI GAATGC 1 cut(s) 653
BsnI GGCC 2 cut(s) 417, 547
Bso31I GGTCTC 2 cut(s) 205, 205
Bsp119I TTCGAA 1 cut(s) 88
Bsp1407I TGTACA 1 cut(s) 20
Bsp143I GATC 3 cut(s) 128, 183, 325
BspACI CCGC 8 cut(s) 36, 232, 244, 266, 287, 541, 814, 855
BspANI GGCC 2 cut(s) 417, 547
BspCNI CTCAG 4 cut(s) 475, 673, 745, 874
BspFNI CGCG 2 cut(s) 232, 493
BspLI GGNNCC 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 280
BspPI GGATC 2 cut(s) 123, 178
BspT104I TTCGAA 1 cut(s) 88
BspTNI GGTCTC 2 cut(s) 205, 205
BsrGI TGTACA 1 cut(s) 20
BsrI ACTGG 2 cut(s) 187, 415
BssMI GATC 3 cut(s) 128, 183, 325
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 2 cut(s) 383, 691
Bst6I CTCTTC 3 cut(s) 53, 90, 864
BstAPI GCANNNNNTGC 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 20
BstBI TTCGAA 1 cut(s) 88
BstC8I GCNNGC 2 cut(s) 230, 633
BstDEI CTNAG 4 cut(s) 483, 660, 753, 882
BstF5I GGATG 2 cut(s) 295, 851
BstFNI CGCG 2 cut(s) 232, 493
BstH2I RGCGCY 2 cut(s) 266, 460
BstHHI GCGC 2 cut(s) 265, 459
BstKTI GATC 3 cut(s) 131, 186, 328
BstMAI GTCTC 2 cut(s) 205, 205
BstMBI GATC 3 cut(s) 128, 183, 325
BstMWI GCNNNNNNNGC 2 cut(s) 103, 284
BstNI CCWGG 1 cut(s) 429
BstSCI CCNGG 1 cut(s) 427
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 2 cut(s) 232, 493
BstV1I GCAGC 2 cut(s) 272, 290
BsuRI GGCC 2 cut(s) 417, 547
BtsCI GGATG 2 cut(s) 295, 851
BtsI GCAGTG 2 cut(s) 117, 300
BtsIMutI CAGTG 3 cut(s) 117, 300, 408
Cac8I GCNNGC 2 cut(s) 230, 633
CaiI CAGNNNCTG 1 cut(s) 667
CfoI GCGC 2 cut(s) 265, 459
Cfr13I GGNCC 2 cut(s) 271, 298
CseI GACGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 21, 420
CviQI GTAC 2 cut(s) 21, 420
DdeI CTNAG 4 cut(s) 483, 660, 753, 882
DpnI GATC 3 cut(s) 130, 185, 327
DpnII GATC 3 cut(s) 128, 183, 325
Eam1104I CTCTTC 3 cut(s) 53, 90, 864
EarI CTCTTC 3 cut(s) 53, 90, 864
EciI GGCGGA 1 cut(s) 556
Eco31I GGTCTC 2 cut(s) 205, 205
Eco47I GGWCC 2 cut(s) 271, 298
Eco57I CTGAAG 3 cut(s) 342, 830, 888
EcoRI GAATTC 1 cut(s) 842
EcoRII CCWGG 1 cut(s) 427
FaiI YATR 7 cut(s) 55, 110, 150, 307, 518, 520, 578
FauI CCCGC 3 cut(s) 43, 251, 807
Fnu4HI GCNGC 3 cut(s) 261, 266, 279
FokI GGATG 2 cut(s) 302, 838
Fsp4HI GCNGC 3 cut(s) 261, 266, 279
FspBI CTAG 2 cut(s) 734, 901
GlaI GCGC 2 cut(s) 264, 458
GluI GCNGC 3 cut(s) 261, 266, 279
GsuI CTGGAG 1 cut(s) 432
HaeII RGCGCY 2 cut(s) 266, 460
HaeIII GGCC 2 cut(s) 417, 547
HapII CCGG 1 cut(s) 683
HgaI GACGC 1 cut(s) 112
HhaI GCGC 2 cut(s) 265, 459
Hin6I GCGC 2 cut(s) 263, 457
HinP1I GCGC 2 cut(s) 263, 457
HinfI GANTC 4 cut(s) 353, 479, 562, 585
HpaII CCGG 1 cut(s) 683
HphI GGTGA 3 cut(s) 33, 335, 395
Hpy166II GTNNAC 2 cut(s) 422, 497
Hpy188I TCNGA 7 cut(s) 128, 361, 484, 555, 663, 810, 883
Hpy8I GTNNAC 2 cut(s) 422, 497
Hpy99I CGWCG 2 cut(s) 106, 585
HpyAV CCTTC 4 cut(s) 199, 533, 599, 889
HpyCH4III ACNGT 2 cut(s) 383, 691
HpyCH4V TGCA 4 cut(s) 260, 278, 293, 653
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 284
HpyF3I CTNAG 4 cut(s) 483, 660, 753, 882
HspAI GCGC 2 cut(s) 263, 457
Kzo9I GATC 3 cut(s) 128, 183, 325
LmnI GCTCC 2 cut(s) 454, 601
Lsp1109I GCAGC 2 cut(s) 272, 290
LweI GCATC 2 cut(s) 47, 280
MaeI CTAG 2 cut(s) 734, 901
MaeIII GTNAC 1 cut(s) 383
MalI GATC 3 cut(s) 130, 185, 327
MboI GATC 3 cut(s) 128, 183, 325
MboII GAAGA 7 cut(s) 40, 77, 127, 348, 637, 838, 881
MluCI AATT 4 cut(s) 11, 531, 842, 874
MluI ACGCGT 1 cut(s) 491
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 579
MseI TTAA 3 cut(s) 237, 366, 558
MspA1I CMGCKG 1 cut(s) 268
MspI CCGG 1 cut(s) 683
MspR9I CCNGG 1 cut(s) 429
Mva1269I GAATGC 1 cut(s) 653
MvaI CCWGG 1 cut(s) 429
MvnI CGCG 2 cut(s) 232, 493
MwoI GCNNNNNNNGC 2 cut(s) 103, 284
NdeII GATC 3 cut(s) 128, 183, 325
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 383
NspV TTCGAA 1 cut(s) 88
PctI GAATGC 1 cut(s) 653
PfeI GAWTC 3 cut(s) 353, 562, 585
PkrI GCNGC 3 cut(s) 262, 267, 280
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 2 cut(s) 271, 298
PstI CTGCAG 1 cut(s) 280
PstNI CAGNNNCTG 1 cut(s) 667
RsaI GTAC 2 cut(s) 22, 421
RsaNI GTAC 2 cut(s) 21, 420
SaqAI TTAA 3 cut(s) 237, 366, 558
SatI GCNGC 3 cut(s) 261, 266, 279
Sau3AI GATC 3 cut(s) 128, 183, 325
Sau96I GGNCC 2 cut(s) 271, 298
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 1 cut(s) 429
SfaNI GCATC 2 cut(s) 47, 280
SfcI CTRYAG 1 cut(s) 276
SfuI TTCGAA 1 cut(s) 88
SinI GGWCC 2 cut(s) 271, 298
SmlI CTYRAG 2 cut(s) 373, 707
SmoI CTYRAG 2 cut(s) 373, 707
Sse9I AATT 4 cut(s) 11, 531, 842, 874
SsiI CCGC 8 cut(s) 36, 232, 244, 266, 287, 541, 814, 855
SspI AATATT 1 cut(s) 256
SspMI CTAG 2 cut(s) 734, 901
StyD4I CCNGG 1 cut(s) 427
TaaI ACNGT 2 cut(s) 383, 691
TaqI TCGA 3 cut(s) 88, 101, 203
TasI AATT 4 cut(s) 11, 531, 842, 874
TatI WGTACW 1 cut(s) 20
TauI GCSGC 1 cut(s) 268
TfiI GAWTC 3 cut(s) 353, 562, 585
Tru1I TTAA 3 cut(s) 237, 366, 558
Tru9I TTAA 3 cut(s) 237, 366, 558
TscAI CASTG 3 cut(s) 124, 300, 415
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 2 cut(s) 260, 278
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 2 cut(s) 633, 736
TspGWI ACGGA 1 cut(s) 265
TspRI CASTG 3 cut(s) 124, 300, 415
VpaK11BI GGWCC 2 cut(s) 271, 298
XapI RAATTY 3 cut(s) 531, 842, 874
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XspI CTAG 2 cut(s) 734, 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.