pycom13g07960

pre-mRNA-processing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004435
Physical Location & Seq
Reverse (-)
5277352 .. 5279954
2603 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1827 bp
ATGGATCTTCTCCGAGCATACTCTGATCAAAACGACGACGAATCGAACGAGCCAGAGGAGCACAACCAAAACCCTAAATCCGCACCGTCGTCCCCAGACGCGTCGCCGCCTCGGCTCCTCCCCGCCAAGTCCGCGGCGCCCAATGTCGACGACACTATGCTGGCCCTGGCGGTAGCCGGGGCCAACCGGTCTTCCACCCGCCCCATCGACCCGACCCAACACATCGTCGGTTTCAACCCCACGTACGACCAGCTATGGGCGCCCATCTATGGCCCATCCCACCCGTACGCCAAGGACGGCATCGCCCAGGGGATGCGCAACCACAAGCTCGGCTTCGTGGAGGACGCATCAATTGAGCCCTTCGTGTTCGACGAGCAGTACAACACTTTTCACAAGTATGGGTATGCTGCTGATCCATCGGCCTCTGCTGGGTACAATTATGTTGGAGATTTCGAGGCCTTGCAGAAGAACGACGCCGTTTCTGTGTATAACATTCCCCAGCACGAGCAGAAGAAGCGGAAGATTGAAAAGAGGAAGGAATTGGACGAGAATGAGGGTGAGGATGAAGATATGGACCCCGAGGAGGTCCAGAACCCGGCTAGTGACGTGTGGTTGATGAAGAATAAGAAGAGTCCGTGGGCGGGAAAGAAGGAGGGTCTGCCAACGGAGCTGACTGAGGAGCAGAAGAAGTATGCAGAGGAGTATGCCAAGAAGAAAGGCGAAGAGAAAGGGGCTGGCGATAAGGGTGAGGCTGTGGTTGAGAAGACTACGTTTCATGGGAAGGAAGAGAGGGATTATCAGGGTAGGTCTTGGATTGCACCGCCGAGAGATGCTAAGGCATCTAATGATCACTGTTATATTCCGAAGAGGTTGGTGCACACTTGGAGCGGGCACACGAAGGGTGTGTCTGCTATTAGGTTCTTCCCTAAGTACGGGCATTTGATTCTCTCTGCCGGGATGGATACCAAGGTGAAGATTTGGGATGTTTTCAATACCGGAAAGTGTATGAGAACTTACATGGGTCACTCTAAGGCAGTTCGGGATATTTGTTTCTCTAATGATGGGAGTAGGTTTTTGACTGCTAGTTATGATAAGAATATCAAGTACTGGGATACTGAAACTGGGCAGGTGATATCTACATTCTCAACCGGGAAGGTTCCTTATGTTGTCAAGCTGAATCCAGACGAGGATAAGCAGAATGTATTGTTGGCAGGTATGAGTGATAAGAAGATTGTTCAGTGGGATATGAATGAGGGGAAGATAAATCAAGAATATGATCATCATTTGGGTGCTGTGAATACGATTACGTTTGTGGATAATAACCGTAGGTTTGTTACTTCCAGCGATGACAAGTCACTTCGCGTATGGGAGTTTGGGATCCCTGTGGTTATTAAGTATATTAGTGAGCCTCATATGCATTCTATGCCATCAATTTCACTGCACCCCAATTCCAATTGGCTTGCAGCACAGAGTATGGACAATCAGATTCTGATTTATAGCACTAGGGAGAAGTTTCAGCTCAATAAGAAGAAGAGGTTTGCCGGGCATGTCGTCGCTGGGTATGCTTGCCAAGTCAATTTTTCGCCAGACGGACGGTTTGTTATGTCGGGTGATGGTGAAGGTAGATGCTGGTTTTGGGATTGGAAGACATGCAAAGTCTTCAGAACTCTCAAATGTCATGAAGGGGTATGCATTGGGGCTGCATGGCATCCCTTGGAGCAAAGTAAAGTTGCAACATGTGGCTGGGATGGCTTGATTAAGTACTGGATATCAAACTTAACCTTGTTCACATCTAGGACTAGCTGCATTCCACTTCGGTTTGGATGA

Protein Analysis

609

Amino Acids

68.83

Weight (kDa)

6.48

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40 PF00400 290 - 328 9.9e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 291 - 396 2.2e-18 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 291 - 383 1.6e-17 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 291 - 457 7.4e-10 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 291 - 456 7e-16 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 292 - 447 2.3e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 292 - 381 1.4e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 294 - 366 6.5e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 295 - 589 8.2e-53 WDR5 beta-propeller domain
EIF3I PF24805 296 - 397 3.8e-13 EIF3I
WD40_Prp19 PF24814 298 - 383 1.2e-12 Prp19 WD40 domain
WDR55 PF24796 301 - 458 2.6e-17 WDR55
Beta-prop_WDR3_2nd PF25172 310 - 381 3.5e-10 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 313 - 457 8.2e-11 TEP-1 second beta-propeller
Beta-prop_ACSF4 PF13570 331 - 560 8.9e-06 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 331 - 397 5.4e-11 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 333 - 371 2.3e-10 WD domain, G-beta repeat
WD40_RFWD3 PF23419 342 - 417 8.4e-08 E3 ubiquitin-protein ligase RFWD3 WD40 domain
WD40_Gbeta PF25391 356 - 458 1.9e-11 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 357 - 457 8e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 357 - 454 8.8e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 373 - 458 1.2e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 378 - 500 7.4e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 389 - 562 1.4e-22 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 398 - 584 3.9e-15 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 399 - 501 1.2e-06 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 400 - 598 9.3e-09 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 401 - 562 8.4e-08 EIF3I
Beta-prop_THOC3 PF25174 401 - 589 4e-22 THOC3 beta-propeller domain
WDR90_beta-prop_4th PF23342 432 - 566 1.2e-06 WDR90, 4th beta-propeller
WD40_CDC20-Fz PF24807 469 - 591 8.6e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 471 - 589 1.2e-08 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 473 - 554 2.2e-08 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 476 - 589 6.2e-07 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1117
Acc16I TGCGCA 1 cut(s) 317
Acc36I ACCTGC 2 cut(s) 1117, 1202
AccB1I GGYRCC 2 cut(s) 136, 259
AccBSI CCGCTC 1 cut(s) 888
AccI GTMKAC 1 cut(s) 147
AccII CGCG 3 cut(s) 101, 134, 1362
AclWI GGATC 4 cut(s) 12, 407, 1372, 1385
AcuI CTGAAG 1 cut(s) 1645
AcyI GRCGYC 3 cut(s) 137, 260, 474
AfaI GTAC 7 cut(s) 245, 287, 380, 434, 932, 1106, 1763
AfiI CCNNNNNNNGG 7 cut(s) 256, 269, 429, 583, 595, 641, 932
AflIII ACRYGT 3 cut(s) 99, 606, 1736
AgeI ACCGGT 1 cut(s) 186
AgsI TTSAA 3 cut(s) 235, 527, 991
AjiI CACGTC 1 cut(s) 607
AjnI CCWGG 2 cut(s) 165, 306
AjuI GAANNNNNNNTTGG 2 cut(s) 1190, 1222
AluBI AGCT 6 cut(s) 253, 328, 670, 1174, 1519, 1803
AluI AGCT 6 cut(s) 253, 328, 670, 1174, 1519, 1803
Alw21I GWGCWC 2 cut(s) 63, 879
Alw44I GTGCAC 1 cut(s) 875
AlwI GGATC 4 cut(s) 12, 407, 1372, 1385
AlwNI CAGNNNCTG 1 cut(s) 1489
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 5 cut(s) 162, 180, 271, 420, 456
ApaLI GTGCAC 1 cut(s) 875
ApeKI GCWGC 4 cut(s) 407, 1463, 1700, 1803
ArsI GACNNNNNNTTYG 4 cut(s) 890, 922, 1580, 1612
AsiGI ACCGGT 1 cut(s) 186
AspLEI GCGC 3 cut(s) 139, 262, 318
AspS9I GGNCC 5 cut(s) 163, 180, 272, 574, 586
AsuC2I CCSGG 5 cut(s) 178, 596, 955, 1150, 1543
AsuHPI GGTGA 6 cut(s) 569, 758, 982, 1141, 1622, 1628
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 2 cut(s) 574, 586
BaeGI GKGCMC 2 cut(s) 879, 894
BaeI ACNNNNGTAYC 2 cut(s) 954, 987
BamHI GGATCC 1 cut(s) 1377
BanI GGYRCC 2 cut(s) 136, 259
BanII GRGCYC 1 cut(s) 360
BauI CACGAG 1 cut(s) 503
BbsI GAAGAC 4 cut(s) 183, 770, 1651, 1652
Bbv12I GWGCWC 2 cut(s) 63, 879
BbvI GCAGC 4 cut(s) 394, 1475, 1687, 1790
BccI CCATC 9 cut(s) 212, 272, 283, 424, 952, 1055, 1435, 1607, 1742
BceAI ACGGC 2 cut(s) 313, 461
BciT130I CCWGG 2 cut(s) 167, 308
BciVI GTATCC 2 cut(s) 955, 1105
BclI TGATCA 3 cut(s) 25, 847, 1276
BcnI CCSGG 5 cut(s) 178, 596, 955, 1150, 1543
BfaI CTAG 5 cut(s) 600, 1083, 1503, 1794, 1800
BfoI RGCGCY 2 cut(s) 140, 263
BfuAI ACCTGC 2 cut(s) 1117, 1202
BfuI GTATCC 2 cut(s) 955, 1105
BglI GCCNNNNNGGC 1 cut(s) 112
BisI GCNGC 6 cut(s) 107, 135, 408, 1464, 1701, 1804
BlsI GCNGC 6 cut(s) 108, 136, 409, 1465, 1702, 1805
BmcAI AGTACT 2 cut(s) 1106, 1763
Bme1390I CCNGG 7 cut(s) 167, 178, 308, 596, 955, 1150, 1543
Bme18I GGWCC 2 cut(s) 574, 586
BmeT110I CYCGRG 1 cut(s) 578
BmgBI CACGTC 1 cut(s) 607
BmgT120I GGNCC 5 cut(s) 163, 180, 272, 574, 586
BmiI GGNNCC 7 cut(s) 116, 138, 181, 261, 576, 1158, 1379
BmrFI CCNGG 7 cut(s) 167, 178, 308, 596, 955, 1150, 1543
BmrI ACTGGG 2 cut(s) 1117, 1131
BmsI GCATC 7 cut(s) 303, 309, 356, 820, 848, 1616, 1717
BmuI ACTGGG 2 cut(s) 1117, 1131
BpiI GAAGAC 4 cut(s) 183, 770, 1651, 1652
Bpu10I CCTNAGC 1 cut(s) 834
BpuMI CCSGG 5 cut(s) 178, 596, 955, 1150, 1543
BsaAI YACGTR 1 cut(s) 243
BsaHI GRCGYC 3 cut(s) 137, 260, 474
BsaWI WCCGGW 2 cut(s) 186, 995
Bsc4I CCNNNNNNNGG 7 cut(s) 256, 269, 429, 583, 595, 641, 932
Bse118I RCCGGY 1 cut(s) 186
Bse1I ACTGG 3 cut(s) 1112, 1126, 1769
BseBI CCWGG 2 cut(s) 167, 308
BseGI GGATG 7 cut(s) 275, 318, 568, 963, 988, 1708, 1753
BseLI CCNNNNNNNGG 7 cut(s) 256, 269, 429, 583, 595, 641, 932
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 3 cut(s) 1112, 1126, 1769
BseRI GAGGAG 5 cut(s) 71, 107, 596, 692, 713
BseSI GKGCMC 2 cut(s) 879, 894
BseXI GCAGC 4 cut(s) 394, 1475, 1687, 1790
BseYI CCCAGC 4 cut(s) 428, 498, 1556, 1743
BsgI GTGCAG 1 cut(s) 1424
Bsh1236I CGCG 3 cut(s) 101, 134, 1362
BshFI GGCC 5 cut(s) 164, 182, 273, 422, 458
BshNI GGYRCC 2 cut(s) 136, 259
BshTI ACCGGT 1 cut(s) 186
BsiHKAI GWGCWC 2 cut(s) 63, 879
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 7 cut(s) 177, 187, 596, 954, 996, 1149, 1542
BsiWI CGTACG 2 cut(s) 243, 285
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 7 cut(s) 256, 269, 429, 583, 595, 641, 932
BsmFI GGGAC 1 cut(s) 76
BsmI GAATGC 2 cut(s) 1417, 1806
BsnI GGCC 5 cut(s) 164, 182, 273, 422, 458
BsoBI CYCGRG 1 cut(s) 578
Bsp1286I GDGCHC 4 cut(s) 63, 360, 879, 894
Bsp143I GATC 6 cut(s) 4, 25, 412, 847, 1276, 1377
BspANI GGCC 5 cut(s) 164, 182, 273, 422, 458
BspCNI CTCAG 1 cut(s) 667
BspFNI CGCG 3 cut(s) 101, 134, 1362
BspHI TCATGA 1 cut(s) 1678
BspLI GGNNCC 7 cut(s) 116, 138, 181, 261, 576, 1158, 1379
BspMI ACCTGC 2 cut(s) 1117, 1202
BspPI GGATC 4 cut(s) 12, 407, 1372, 1385
BspT107I GGYRCC 2 cut(s) 136, 259
BsrBI CCGCTC 1 cut(s) 888
BsrFI RCCGGY 1 cut(s) 186
BsrI ACTGG 3 cut(s) 1112, 1126, 1769
BssAI RCCGGY 1 cut(s) 186
BssMI GATC 6 cut(s) 4, 25, 412, 847, 1276, 1377
BssNI GRCGYC 3 cut(s) 137, 260, 474
BssSI CACGAG 1 cut(s) 503
BssT1I CCWWGG 3 cut(s) 291, 966, 1713
Bst2BI CACGAG 1 cut(s) 503
Bst2UI CCWGG 2 cut(s) 167, 308
Bst4CI ACNGT 4 cut(s) 87, 854, 1325, 1596
Bst6I CTCTTC 5 cut(s) 623, 717, 780, 860, 1526
BstACI GRCGYC 3 cut(s) 137, 260, 474
BstAPI GCANNNNNTGC 1 cut(s) 1423
BstBAI YACGTR 1 cut(s) 243
BstC8I GCNNGC 5 cut(s) 162, 736, 890, 1461, 1567
BstDEI CTNAG 4 cut(s) 675, 834, 927, 1029
BstDSI CCRYGG 2 cut(s) 132, 635
BstF5I GGATG 7 cut(s) 275, 318, 568, 963, 988, 1708, 1753
BstFNI CGCG 3 cut(s) 101, 134, 1362
BstH2I RGCGCY 2 cut(s) 140, 263
BstHHI GCGC 3 cut(s) 139, 262, 318
BstKTI GATC 6 cut(s) 7, 28, 415, 850, 1279, 1380
BstMBI GATC 6 cut(s) 4, 25, 412, 847, 1276, 1377
BstNI CCWGG 2 cut(s) 167, 308
BstNSI RCATGY 3 cut(s) 1550, 1653, 1740
BstSCI CCNGG 7 cut(s) 165, 176, 306, 594, 953, 1148, 1541
BstSLI GKGCMC 2 cut(s) 879, 894
BstUI CGCG 3 cut(s) 101, 134, 1362
BstV1I GCAGC 4 cut(s) 394, 1475, 1687, 1790
BstV2I GAAGAC 4 cut(s) 183, 770, 1651, 1652
BstX2I RGATCY 2 cut(s) 4, 1377
BstYI RGATCY 2 cut(s) 4, 1377
BsuI GTATCC 2 cut(s) 955, 1105
BsuRI GGCC 5 cut(s) 164, 182, 273, 422, 458
BtgI CCRYGG 2 cut(s) 132, 635
BtgZI GCGATG 2 cut(s) 286, 1359
BtrI CACGTC 1 cut(s) 607
BtsCI GGATG 7 cut(s) 275, 318, 568, 963, 988, 1708, 1753
BtsI GCAGTG 1 cut(s) 1436
BtsIMutI CAGTG 3 cut(s) 850, 1244, 1436
BveI ACCTGC 2 cut(s) 1117, 1202
Cac8I GCNNGC 5 cut(s) 162, 736, 890, 1461, 1567
CaiI CAGNNNCTG 1 cut(s) 1489
CciI TCATGA 1 cut(s) 1678
CfoI GCGC 3 cut(s) 139, 262, 318
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 5 cut(s) 163, 180, 272, 574, 586
Cfr42I CCGCGG 1 cut(s) 135
CseI GACGC 4 cut(s) 90, 107, 353, 482
Csp6I GTAC 7 cut(s) 244, 286, 379, 433, 931, 1105, 1762
CspAI ACCGGT 1 cut(s) 186
CviAII CATG 7 cut(s) 776, 1018, 1547, 1650, 1679, 1704, 1737
CviQI GTAC 7 cut(s) 244, 286, 379, 433, 931, 1105, 1762
DdeI CTNAG 4 cut(s) 675, 834, 927, 1029
DinI GGCGCC 2 cut(s) 138, 261
DpnI GATC 6 cut(s) 6, 27, 414, 849, 1278, 1379
DpnII GATC 6 cut(s) 4, 25, 412, 847, 1276, 1377
Eam1104I CTCTTC 5 cut(s) 623, 717, 780, 860, 1526
EarI CTCTTC 5 cut(s) 623, 717, 780, 860, 1526
Eco130I CCWWGG 3 cut(s) 291, 966, 1713
Eco147I AGGCCT 1 cut(s) 458
Eco24I GRGCYC 1 cut(s) 360
Eco32I GATATC 2 cut(s) 1134, 1770
Eco47I GGWCC 2 cut(s) 574, 586
Eco57I CTGAAG 1 cut(s) 1645
Eco88I CYCGRG 1 cut(s) 578
EcoRII CCWGG 2 cut(s) 165, 306
EcoRV GATATC 2 cut(s) 1134, 1770
EcoT14I CCWWGG 3 cut(s) 291, 966, 1713
EcoT22I ATGCAT 2 cut(s) 1419, 1694
EcoT38I GRGCYC 1 cut(s) 360
EgeI GGCGCC 2 cut(s) 138, 261
EheI GGCGCC 2 cut(s) 138, 261
ErhI CCWWGG 3 cut(s) 291, 966, 1713
FaeI CATG 7 cut(s) 779, 1021, 1550, 1653, 1682, 1707, 1740
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 1 cut(s) 76
FatI CATG 7 cut(s) 775, 1017, 1546, 1649, 1678, 1703, 1736
FauI CCCGC 4 cut(s) 130, 206, 634, 881
FauNDI CATATG 1 cut(s) 1413
FbaI TGATCA 3 cut(s) 25, 847, 1276
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 6 cut(s) 107, 135, 408, 1464, 1701, 1804
FokI GGATG 7 cut(s) 262, 325, 575, 970, 995, 1695, 1760
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 6 cut(s) 107, 135, 408, 1464, 1701, 1804
FspBI CTAG 5 cut(s) 600, 1083, 1503, 1794, 1800
FspI TGCGCA 1 cut(s) 317
GlaI GCGC 3 cut(s) 138, 261, 317
GluI GCNGC 6 cut(s) 107, 135, 408, 1464, 1701, 1804
GsaI CCCAGC 4 cut(s) 432, 502, 1560, 1747
HaeII RGCGCY 2 cut(s) 140, 263
HaeIII GGCC 5 cut(s) 164, 182, 273, 422, 458
HapII CCGG 7 cut(s) 177, 187, 596, 954, 996, 1149, 1542
HgaI GACGC 4 cut(s) 90, 107, 353, 482
HhaI GCGC 3 cut(s) 139, 262, 318
Hin1I GRCGYC 3 cut(s) 137, 260, 474
Hin1II CATG 7 cut(s) 779, 1021, 1550, 1653, 1682, 1707, 1740
Hin6I GCGC 3 cut(s) 137, 260, 316
HinP1I GCGC 3 cut(s) 137, 260, 316
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 5 cut(s) 41, 631, 943, 1177, 1486
HpaII CCGG 7 cut(s) 177, 187, 596, 954, 996, 1149, 1542
HphI GGTGA 6 cut(s) 569, 758, 982, 1141, 1622, 1628
Hpy166II GTNNAC 3 cut(s) 148, 877, 1788
Hpy188I TCNGA 6 cut(s) 14, 25, 864, 1485, 1491, 1664
Hpy188III TCNNGA 5 cut(s) 589, 1040, 1181, 1268, 1679
Hpy8I GTNNAC 3 cut(s) 148, 877, 1788
Hpy99I CGWCG 9 cut(s) 38, 41, 91, 106, 152, 230, 374, 476, 1556
HpyAV CCTTC 8 cut(s) 370, 529, 643, 775, 892, 1147, 1613, 1676
HpyCH4III ACNGT 4 cut(s) 87, 854, 1325, 1596
HpyCH4IV ACGT 4 cut(s) 242, 606, 770, 1307
HpyF3I CTNAG 4 cut(s) 675, 834, 927, 1029
HpySE526I ACGT 4 cut(s) 242, 606, 770, 1307
Hsp92I GRCGYC 3 cut(s) 137, 260, 474
Hsp92II CATG 7 cut(s) 779, 1021, 1550, 1653, 1682, 1707, 1740
HspAI GCGC 3 cut(s) 137, 260, 316
KasI GGCGCC 2 cut(s) 136, 259
Ksp22I TGATCA 3 cut(s) 25, 847, 1276
KspI CCGCGG 1 cut(s) 135
Kzo9I GATC 6 cut(s) 4, 25, 412, 847, 1276, 1377
LmnI GCTCC 6 cut(s) 58, 120, 667, 679, 885, 1717
Lsp1109I GCAGC 4 cut(s) 394, 1475, 1687, 1790
LweI GCATC 7 cut(s) 303, 309, 356, 820, 848, 1616, 1717
MaeI CTAG 5 cut(s) 600, 1083, 1503, 1794, 1800
MaeII ACGT 4 cut(s) 242, 606, 770, 1307
MaeIII GTNAC 4 cut(s) 602, 1022, 1333, 1353
MalI GATC 6 cut(s) 6, 27, 414, 849, 1278, 1379
MbiI CCGCTC 1 cut(s) 888
MboI GATC 6 cut(s) 4, 25, 412, 847, 1276, 1377
MfeI CAATTG 2 cut(s) 351, 1453
MflI RGATCY 2 cut(s) 4, 1377
MhlI GDGCHC 4 cut(s) 63, 360, 879, 894
MluCI AATT 7 cut(s) 351, 436, 539, 1431, 1447, 1453, 1576
MluI ACGCGT 1 cut(s) 99
Mly113I GGCGCC 2 cut(s) 137, 260
MlyI GAGTC 1 cut(s) 640
MmeI TCCRAC 1 cut(s) 424
Mph1103I ATGCAT 2 cut(s) 1419, 1694
MseI TTAA 3 cut(s) 1392, 1758, 1778
MslI CAYNNNNRTG 2 cut(s) 396, 1287
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 7 cut(s) 177, 187, 596, 954, 996, 1149, 1542
MspR9I CCNGG 7 cut(s) 167, 178, 308, 596, 955, 1150, 1543
MunI CAATTG 2 cut(s) 351, 1453
Mva1269I GAATGC 2 cut(s) 1417, 1806
MvaI CCWGG 2 cut(s) 167, 308
MvnI CGCG 3 cut(s) 101, 134, 1362
NarI GGCGCC 2 cut(s) 137, 260
NciI CCSGG 5 cut(s) 178, 596, 955, 1150, 1543
NdeI CATATG 1 cut(s) 1413
NdeII GATC 6 cut(s) 4, 25, 412, 847, 1276, 1377
NlaIII CATG 7 cut(s) 779, 1021, 1550, 1653, 1682, 1707, 1740
NlaIV GGNNCC 7 cut(s) 116, 138, 181, 261, 576, 1158, 1379
NmeAIII GCCGAG 3 cut(s) 91, 309, 849
NmuCI GTSAC 3 cut(s) 602, 1022, 1353
NsbI TGCGCA 1 cut(s) 317
NsiI ATGCAT 2 cut(s) 1419, 1694
NspI RCATGY 3 cut(s) 1550, 1653, 1740
PagI TCATGA 1 cut(s) 1678
PaqCI CACCTGC 1 cut(s) 1117
PasI CCCWGGG 1 cut(s) 307
PceI AGGCCT 1 cut(s) 458
PciI ACATGT 1 cut(s) 1736
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 369
PctI GAATGC 2 cut(s) 1417, 1806
PfeI GAWTC 4 cut(s) 41, 943, 1177, 1486
Pfl23II CGTACG 2 cut(s) 243, 285
PinAI ACCGGT 1 cut(s) 186
PkrI GCNGC 6 cut(s) 108, 136, 409, 1465, 1702, 1805
PleI GAGTC 1 cut(s) 639
PluTI GGCGCC 2 cut(s) 140, 263
PpsI GAGTC 1 cut(s) 639
Ppu21I YACGTR 1 cut(s) 243
PscI ACATGT 1 cut(s) 1736
Psp6I CCWGG 2 cut(s) 165, 306
PspFI CCCAGC 4 cut(s) 428, 498, 1556, 1743
PspGI CCWGG 2 cut(s) 165, 306
PspLI CGTACG 2 cut(s) 243, 285
PspN4I GGNNCC 7 cut(s) 116, 138, 181, 261, 576, 1158, 1379
PspPI GGNCC 5 cut(s) 163, 180, 272, 574, 586
PstNI CAGNNNCTG 1 cut(s) 1489
PsuI RGATCY 2 cut(s) 4, 1377
RsaI GTAC 7 cut(s) 245, 287, 380, 434, 932, 1106, 1763
RsaNI GTAC 7 cut(s) 244, 286, 379, 433, 931, 1105, 1762
RseI CAYNNNNRTG 2 cut(s) 396, 1287
SacII CCGCGG 1 cut(s) 135
SalI GTCGAC 1 cut(s) 146
SaqAI TTAA 3 cut(s) 1392, 1758, 1778
SatI GCNGC 6 cut(s) 107, 135, 408, 1464, 1701, 1804
Sau3AI GATC 6 cut(s) 4, 25, 412, 847, 1276, 1377
Sau96I GGNCC 5 cut(s) 163, 180, 272, 574, 586
ScaI AGTACT 2 cut(s) 1106, 1763
SchI GAGTC 1 cut(s) 640
ScrFI CCNGG 7 cut(s) 167, 178, 308, 596, 955, 1150, 1543
SduI GDGCHC 4 cut(s) 63, 360, 879, 894
SfaNI GCATC 7 cut(s) 303, 309, 356, 820, 848, 1616, 1717
SfoI GGCGCC 2 cut(s) 138, 261
Sfr303I CCGCGG 1 cut(s) 135
SgrBI CCGCGG 1 cut(s) 135
SinI GGWCC 2 cut(s) 574, 586
SmiMI CAYNNNNRTG 2 cut(s) 396, 1287
Sse9I AATT 7 cut(s) 351, 436, 539, 1431, 1447, 1453, 1576
SseBI AGGCCT 1 cut(s) 458
SspDI GGCGCC 2 cut(s) 136, 259
SspMI CTAG 5 cut(s) 600, 1083, 1503, 1794, 1800
StuI AGGCCT 1 cut(s) 458
StyD4I CCNGG 7 cut(s) 165, 176, 306, 594, 953, 1148, 1541
StyI CCWWGG 3 cut(s) 291, 966, 1713
TaaI ACNGT 4 cut(s) 87, 854, 1325, 1596
TaiI ACGT 4 cut(s) 245, 609, 773, 1310
TaqI TCGA 5 cut(s) 44, 147, 207, 369, 453
TasI AATT 7 cut(s) 351, 436, 539, 1431, 1447, 1453, 1576
TatI WGTACW 3 cut(s) 378, 1104, 1761
TauI GCSGC 2 cut(s) 109, 137
TfiI GAWTC 4 cut(s) 41, 943, 1177, 1486
Tru1I TTAA 3 cut(s) 1392, 1758, 1778
Tru9I TTAA 3 cut(s) 1392, 1758, 1778
TscAI CASTG 3 cut(s) 857, 1244, 1443
TseFI GTSAC 3 cut(s) 602, 1022, 1353
TseI GCWGC 4 cut(s) 407, 1463, 1700, 1803
Tsp45I GTSAC 3 cut(s) 602, 1022, 1353
TspDTI ATGAA 5 cut(s) 579, 632, 764, 1262, 1695
TspGWI ACGGA 3 cut(s) 624, 680, 1605
TspRI CASTG 3 cut(s) 857, 1244, 1443
VneI GTGCAC 1 cut(s) 875
VpaK11BI GGWCC 2 cut(s) 574, 586
XceI RCATGY 3 cut(s) 1550, 1653, 1740
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 5 cut(s) 600, 1083, 1503, 1794, 1800
ZrmI AGTACT 2 cut(s) 1106, 1763
Zsp2I ATGCAT 2 cut(s) 1419, 1694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.