pycom13g10690

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005407
Physical Location & Seq
Forward (+)
7200518 .. 7201282
765 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
ATGGATCCAATCAAGGCTGAAAGGCTTCGCGCCATGAAATGCCACAAGAAGGAGCAGACCTACTACAACAACAATAGTCAGTTTCTTTACAGTCTTTTTGTTCATTCCCTTATTGCAGTGACATGCAGTTTGCTTTGTTCATACTCTTATTCGTTTCCTTCTCTCTTCACTATCAAACACTTGCTATTTATGTCTCTTCCGAACTTTTGCTCGGGGTTTATCAACCCCAAGTGCCTCTTCATTGTGGTGAATTTTATAATCGTATTCCTTATTGGAGAATCAAGGCTCAGCGGTGGCAAGCAATTGTCTCCAGTCACCGAGATGTACAACGAGTATATTGAGAGAAGTCGAAGTCTCAGGAGACCACCTTCCACGTTTCAAGGGAAGAAAGAGGAGGGGACCGTGCCGGAAATGTTGAATCCGATAAACGAGGGCAATGCGGAGAGTAATGAAGATAAAGAAGTTGATGAAGGTAAAGAAGATGATGATCAAGATCATGAAGAAGAAGAAGACTTTAAAGAATGTGGAGATAATGATGAAGAAGAAGGGGAAGAAGAAAAGGGGGAAGAAAGAGAAGAAGAAAAAAAGGAAGAAACGGAGGAAGAAAAAGAAGAAGAAAAAAAGGAGGAAATAGAGGAAGAAAAGGAAGAAGAAAAGGCTGGAATACCTGCTGAGGAACTAAACAAAAGAGTTGAAGACTTCATTGCAAGGGTTAATAAGCAAAGGTGGCTTGAGGCTAGATTTTTGGTTTGTTGTAAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408

Protein Analysis

255

Amino Acids

29.67

Weight (kDa)

4.61

Isoelectric Point (pI)

76.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 75 - 96 2.6e-07 Domain of unknown function (DUF4408)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 676
AccII CGCG 1 cut(s) 30
AciI CCGC 2 cut(s) 291, 440
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 1 cut(s) 250
AfaI GTAC 1 cut(s) 326
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 3 cut(s) 380, 418, 695
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
Alw26I GTCTC 4 cut(s) 198, 312, 355, 359
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 211
ApoI RAATTY 1 cut(s) 250
ArsI GACNNNNNNTTYG 2 cut(s) 112, 144
Asp700I GAANNNNTTC 1 cut(s) 24
AspLEI GCGC 1 cut(s) 32
AspS9I GGNCC 1 cut(s) 399
AsuHPI GGTGA 2 cut(s) 259, 307
AvaI CYCGRG 1 cut(s) 211
AvaII GGWCC 1 cut(s) 399
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 2 cut(s) 516, 702
BbvCI CCTCAGC 1 cut(s) 672
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BclI TGATCA 1 cut(s) 487
BcoDI GTCTC 4 cut(s) 198, 312, 355, 359
BfaI CTAG 1 cut(s) 738
BfuAI ACCTGC 1 cut(s) 676
BlpI GCTNAGC 1 cut(s) 287
Bme18I GGWCC 1 cut(s) 399
BmeT110I CYCGRG 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 399
BmiI GGNNCC 2 cut(s) 6, 400
BpiI GAAGAC 2 cut(s) 516, 702
BpmI CTGGAG 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 672
Bpu1102I GCTNAGC 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 752
BsaBI GATNNNNATC 2 cut(s) 486, 492
BsaI GGTCTC 1 cut(s) 355
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse1I ACTGG 1 cut(s) 311
Bse3DI GCAATG 2 cut(s) 442, 702
Bse8I GATNNNNATC 2 cut(s) 486, 492
BseJI GATNNNNATC 2 cut(s) 486, 492
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMI GCAATG 2 cut(s) 442, 702
BseMII CTCAG 3 cut(s) 301, 370, 663
BseNI ACTGG 1 cut(s) 311
BseRI GAGGAG 1 cut(s) 407
Bsh1236I CGCG 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 211
BsiSI CCGG 1 cut(s) 407
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 4 cut(s) 198, 312, 355, 359
BsmFI GGGAC 1 cut(s) 412
Bso31I GGTCTC 1 cut(s) 355
BsoBI CYCGRG 1 cut(s) 211
Bsp1407I TGTACA 1 cut(s) 324
Bsp143I GATC 3 cut(s) 4, 487, 493
Bsp1720I GCTNAGC 1 cut(s) 287
BspACI CCGC 2 cut(s) 291, 440
BspCNI CTCAG 3 cut(s) 300, 369, 664
BspFNI CGCG 1 cut(s) 30
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 2 cut(s) 6, 400
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 355
BsrDI GCAATG 2 cut(s) 442, 702
BsrGI TGTACA 1 cut(s) 324
BsrI ACTGG 1 cut(s) 311
BssMI GATC 3 cut(s) 4, 487, 493
Bst4CI ACNGT 2 cut(s) 92, 403
Bst6I CTCTTC 3 cut(s) 170, 201, 242
BstAUI TGTACA 1 cut(s) 324
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 3 cut(s) 287, 356, 672
BstFNI CGCG 1 cut(s) 30
BstHHI GCGC 1 cut(s) 32
BstKTI GATC 3 cut(s) 7, 490, 496
BstMAI GTCTC 4 cut(s) 198, 312, 355, 359
BstMBI GATC 3 cut(s) 4, 487, 493
BstMWI GCNNNNNNNGC 1 cut(s) 727
BstNSI RCATGY 1 cut(s) 126
BstUI CGCG 1 cut(s) 30
BstV2I GAAGAC 2 cut(s) 516, 702
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 1 cut(s) 123
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 1 cut(s) 299
CciI TCATGA 1 cut(s) 496
CfoI GCGC 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 399
Csp6I GTAC 1 cut(s) 325
CviAII CATG 4 cut(s) 34, 123, 497, 762
CviJI RGCY 6 cut(s) 17, 25, 286, 659, 730, 737
CviKI_1 RGCY 6 cut(s) 17, 25, 286, 659, 730, 737
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 3 cut(s) 287, 356, 672
DpnI GATC 3 cut(s) 6, 489, 495
DpnII GATC 3 cut(s) 4, 487, 493
DraI TTTAAA 1 cut(s) 517
Eam1104I CTCTTC 3 cut(s) 170, 201, 242
EarI CTCTTC 3 cut(s) 170, 201, 242
Eco31I GGTCTC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 399
Eco88I CYCGRG 1 cut(s) 211
FaeI CATG 4 cut(s) 37, 126, 500, 765
FaiI YATR 8 cut(s) 35, 124, 142, 191, 257, 336, 498, 763
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FaqI GGGAC 1 cut(s) 412
FatI CATG 4 cut(s) 33, 122, 496, 761
FbaI TGATCA 1 cut(s) 487
FspBI CTAG 1 cut(s) 738
GlaI GCGC 1 cut(s) 31
GsuI CTGGAG 1 cut(s) 294
HapII CCGG 1 cut(s) 407
HhaI GCGC 1 cut(s) 32
Hin1II CATG 4 cut(s) 37, 126, 500, 765
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HinfI GANTC 2 cut(s) 278, 418
HpaII CCGG 1 cut(s) 407
HphI GGTGA 2 cut(s) 259, 307
Hpy188I TCNGA 2 cut(s) 201, 423
Hpy188III TCNNGA 3 cut(s) 358, 491, 497
HpyAV CCTTC 5 cut(s) 43, 168, 378, 464, 539
HpyCH4III ACNGT 2 cut(s) 92, 403
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 3 cut(s) 116, 126, 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 727
HpyF3I CTNAG 3 cut(s) 287, 356, 672
HpySE526I ACGT 1 cut(s) 374
Hsp92II CATG 4 cut(s) 37, 126, 500, 765
HspAI GCGC 1 cut(s) 30
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 3 cut(s) 4, 487, 493
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 5 cut(s) 324, 343, 420, 645, 681
MaeI CTAG 1 cut(s) 738
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 2 cut(s) 118, 313
MalI GATC 3 cut(s) 6, 489, 495
MboI GATC 3 cut(s) 4, 487, 493
MfeI CAATTG 1 cut(s) 302
MflI RGATCY 1 cut(s) 4
MluCI AATT 2 cut(s) 250, 302
MnlI CCTC 9 cut(s) 245, 385, 388, 424, 592, 619, 628, 667, 727
MroXI GAANNNNTTC 1 cut(s) 24
MseI TTAA 2 cut(s) 516, 714
MslI CAYNNNNRTG 2 cut(s) 245, 320
MspA1I CMGCKG 1 cut(s) 291
MspI CCGG 1 cut(s) 407
MunI CAATTG 1 cut(s) 302
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 1 cut(s) 727
NdeII GATC 3 cut(s) 4, 487, 493
NlaIII CATG 4 cut(s) 37, 126, 500, 765
NlaIV GGNNCC 2 cut(s) 6, 400
NmuCI GTSAC 2 cut(s) 118, 313
NspI RCATGY 1 cut(s) 126
PagI TCATGA 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 24
PfeI GAWTC 2 cut(s) 278, 418
PsiI TTATAA 1 cut(s) 257
PspN4I GGNNCC 2 cut(s) 6, 400
PspPI GGNCC 1 cut(s) 399
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
RseI CAYNNNNRTG 2 cut(s) 245, 320
SaqAI TTAA 2 cut(s) 516, 714
Sau3AI GATC 3 cut(s) 4, 487, 493
Sau96I GGNCC 1 cut(s) 399
SetI ASST 6 cut(s) 62, 370, 377, 475, 670, 728
SinI GGWCC 1 cut(s) 399
SmiMI CAYNNNNRTG 2 cut(s) 245, 320
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
Sse9I AATT 2 cut(s) 250, 302
SsiI CCGC 2 cut(s) 291, 440
SspMI CTAG 1 cut(s) 738
TaaI ACNGT 2 cut(s) 92, 403
TaiI ACGT 1 cut(s) 377
TaqI TCGA 1 cut(s) 349
TasI AATT 2 cut(s) 250, 302
TatI WGTACW 1 cut(s) 324
TfiI GAWTC 2 cut(s) 278, 418
Tru1I TTAA 2 cut(s) 516, 714
Tru9I TTAA 2 cut(s) 516, 714
TscAI CASTG 1 cut(s) 123
TseFI GTSAC 2 cut(s) 118, 313
Tsp45I GTSAC 2 cut(s) 118, 313
TspDTI ATGAA 9 cut(s) 50, 92, 129, 229, 465, 483, 513, 552, 691
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 399
XapI RAATTY 1 cut(s) 250
XceI RCATGY 1 cut(s) 126
XmnI GAANNNNTTC 1 cut(s) 24
XspI CTAG 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.