pycom13g13620

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003855
Physical Location & Seq
Forward (+)
9676063 .. 9677173
1111 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 804 bp
ATGAGGGCGTGCGCCATGTACTGTTCTAACTTGCAGGTACCTAAGTTTGGAGTTCAGTCACCATCTCTTCGATTGGCATCTTTACGAAGAAGACTAACAACAACAACCGCTTTAAGCAAATCACCTTCGAAATCCTTCAGAAGCATACAATACTCCGATGCTCAAGCAACGATTTTGGATTTCGGAGTTTTCAAGGATCTTGTGAAAATATTTGTTCTAGACCTTGAAACCACCGGACTTTGCAGAAGGATAGGACATGTAACTGAAATCGCACTCCGCGATCTTCAAGGCGGCAAGAACAGTTGCTTTCAAACCCTGGTGAACCCTCAACAACGTGTTTCAAATTCTAGCATCCACGGTATAACAACCGACATGGTGACACATTCAGATGTCCCGAGGATGGTGGAGTTGATTCCGATTCTTCTCGAATACATCAGAAGCCGGCAAGGACCTGGAGTGCAAGTTTTATTTGCAGCTCACAATGCACGGCGTTTCGACGTGCCCTTCTTAATCAAGGAATTCAGCTCCTGCTCGTTTGAAATTCCTCCTGACTGGCTGTTTCTGGACACCCTCGCACTGTCGCATGAGTTAAGGAAATTATACGGGCCAAAGGTTCCTCCAAAGATCTCTCTTGAGGGACTTAGTGAGTTCTACAAGATCACTAGAGAAGGTCCAAAACACAGGGCCATGGCAGATGTGAACTTGCTGACATCTATACTTCCAAACATGACTTCTGACCTCAACCTCGGAGTCGCGGACCTTTTGCAGAGGAGTTTCAAGGCTTCTGTCTGCAGTTTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T

Protein Analysis

268

Amino Acids

29.86

Weight (kDa)

9.41

Isoelectric Point (pI)

51.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 72 - 237 2.2e-13 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 25
Acc65I GGTACC 1 cut(s) 37
AccB1I GGYRCC 1 cut(s) 37
AccII CGCG 2 cut(s) 279, 755
AciI CCGC 4 cut(s) 108, 277, 291, 755
AclWI GGATC 1 cut(s) 204
AcsI RAATTY 3 cut(s) 343, 518, 540
AcuI CTGAAG 1 cut(s) 121
AfaI GTAC 2 cut(s) 20, 39
AfiI CCNNNNNNNGG 2 cut(s) 47, 400
AflIII ACRYGT 2 cut(s) 256, 334
AgsI TTSAA 7 cut(s) 193, 227, 287, 311, 342, 539, 778
AjiI CACGTC 1 cut(s) 499
AjnI CCWGG 2 cut(s) 315, 451
AjuI GAANNNNNNNTTGG 2 cut(s) 715, 747
AluBI AGCT 2 cut(s) 476, 525
AluI AGCT 2 cut(s) 476, 525
AlwI GGATC 1 cut(s) 204
AlwNI CAGNNNCTG 1 cut(s) 528
Ama87I CYCGRG 1 cut(s) 394
AoxI GGCC 2 cut(s) 605, 684
ApeKI GCWGC 1 cut(s) 473
ApoI RAATTY 3 cut(s) 343, 518, 540
Asp700I GAANNNNTTC 1 cut(s) 134
Asp718I GGTACC 1 cut(s) 37
AspLEI GCGC 1 cut(s) 14
AspS9I GGNCC 5 cut(s) 449, 605, 671, 684, 757
AsuHPI GGTGA 4 cut(s) 51, 114, 331, 388
AsuII TTCGAA 1 cut(s) 128
AvaI CYCGRG 1 cut(s) 394
AvaII GGWCC 3 cut(s) 449, 671, 757
BaeGI GKGCMC 1 cut(s) 504
BaeI ACNNNNGTAYC 2 cut(s) 29, 62
BanI GGYRCC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 97
BbvI GCAGC 1 cut(s) 485
BccI CCATC 2 cut(s) 70, 394
BceAI ACGGC 1 cut(s) 503
BciT130I CCWGG 2 cut(s) 317, 453
BfaI CTAG 3 cut(s) 218, 348, 663
BfmI CTRYAG 1 cut(s) 790
BfuAI ACCTGC 1 cut(s) 25
BglII AGATCT 1 cut(s) 624
BisI GCNGC 2 cut(s) 292, 474
BlsI GCNGC 2 cut(s) 293, 475
Bme1390I CCNGG 2 cut(s) 317, 453
Bme18I GGWCC 3 cut(s) 449, 671, 757
BmeT110I CYCGRG 1 cut(s) 394
BmgBI CACGTC 1 cut(s) 499
BmgT120I GGNCC 5 cut(s) 449, 605, 671, 684, 757
BmiI GGNNCC 2 cut(s) 39, 615
BmrFI CCNGG 2 cut(s) 317, 453
BmsI GCATC 3 cut(s) 86, 148, 360
BpiI GAAGAC 1 cut(s) 97
BpmI CTGGAG 1 cut(s) 474
Bpu14I TTCGAA 1 cut(s) 128
BpuEI CTTGAG 2 cut(s) 147, 653
BsaBI GATNNNNATC 1 cut(s) 76
BsaJI CCNNGG 5 cut(s) 315, 355, 395, 687, 745
BsaWI WCCGGW 1 cut(s) 233
BsaXI ACNNNNNCTCC 2 cut(s) 447, 477
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 400
Bse118I RCCGGY 1 cut(s) 441
Bse1I ACTGG 1 cut(s) 557
Bse8I GATNNNNATC 1 cut(s) 76
BseBI CCWGG 2 cut(s) 317, 453
BseDI CCNNGG 5 cut(s) 315, 355, 395, 687, 745
BseGI GGATG 2 cut(s) 351, 405
BseJI GATNNNNATC 1 cut(s) 76
BseLI CCNNNNNNNGG 2 cut(s) 47, 400
BseNI ACTGG 1 cut(s) 557
BseRI GAGGAG 1 cut(s) 784
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 1 cut(s) 485
Bsh1236I CGCG 2 cut(s) 279, 755
BshFI GGCC 2 cut(s) 607, 686
BshNI GGYRCC 1 cut(s) 37
BsiHKCI CYCGRG 1 cut(s) 394
BsiSI CCGG 2 cut(s) 234, 442
BslFI GGGAC 2 cut(s) 377, 651
BslI CCNNNNNNNGG 2 cut(s) 47, 400
BsmFI GGGAC 2 cut(s) 377, 651
BsnI GGCC 2 cut(s) 607, 686
BsoBI CYCGRG 1 cut(s) 394
Bsp119I TTCGAA 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 504
Bsp143I GATC 4 cut(s) 196, 280, 624, 657
Bsp19I CCATGG 1 cut(s) 687
BspACI CCGC 4 cut(s) 108, 277, 291, 755
BspANI GGCC 2 cut(s) 607, 686
BspFNI CGCG 2 cut(s) 279, 755
BspLI GGNNCC 2 cut(s) 39, 615
BspMAI CTGCAG 1 cut(s) 794
BspMI ACCTGC 1 cut(s) 25
BspPI GGATC 1 cut(s) 204
BspT104I TTCGAA 1 cut(s) 128
BspT107I GGYRCC 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 441
BsrI ACTGG 1 cut(s) 557
BssAI RCCGGY 1 cut(s) 441
BssECI CCNNGG 5 cut(s) 315, 355, 395, 687, 745
BssMI GATC 4 cut(s) 196, 280, 624, 657
BssT1I CCWWGG 1 cut(s) 687
Bst2UI CCWGG 2 cut(s) 317, 453
Bst4CI ACNGT 4 cut(s) 23, 302, 359, 579
Bst6I CTCTTC 1 cut(s) 72
BstBI TTCGAA 1 cut(s) 128
BstC8I GCNNGC 2 cut(s) 10, 443
BstDEI CTNAG 2 cut(s) 42, 641
BstDSI CCRYGG 2 cut(s) 355, 687
BstF5I GGATG 2 cut(s) 351, 405
BstFNI CGCG 2 cut(s) 279, 755
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 4 cut(s) 199, 283, 627, 660
BstMBI GATC 4 cut(s) 196, 280, 624, 657
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstNI CCWGG 2 cut(s) 317, 453
BstNSI RCATGY 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 315, 451
BstSFI CTRYAG 1 cut(s) 790
BstSLI GKGCMC 1 cut(s) 504
BstUI CGCG 2 cut(s) 279, 755
BstV1I GCAGC 1 cut(s) 485
BstV2I GAAGAC 1 cut(s) 97
BstX2I RGATCY 2 cut(s) 196, 624
BstYI RGATCY 2 cut(s) 196, 624
BsuRI GGCC 2 cut(s) 607, 686
BtgI CCRYGG 2 cut(s) 355, 687
BtrI CACGTC 1 cut(s) 499
BtsCI GGATG 2 cut(s) 351, 405
BtsIMutI CAGTG 1 cut(s) 575
BveI ACCTGC 1 cut(s) 25
Cac8I GCNNGC 2 cut(s) 10, 443
CaiI CAGNNNCTG 1 cut(s) 528
CfoI GCGC 1 cut(s) 14
Cfr10I RCCGGY 1 cut(s) 441
Cfr13I GGNCC 5 cut(s) 449, 605, 671, 684, 757
Csp6I GTAC 2 cut(s) 19, 38
CviAII CATG 6 cut(s) 16, 257, 373, 584, 688, 727
CviJI RGCY 7 cut(s) 441, 476, 525, 556, 607, 686, 782
CviKI_1 RGCY 7 cut(s) 441, 476, 525, 556, 607, 686, 782
CviQI GTAC 2 cut(s) 19, 38
DdeI CTNAG 2 cut(s) 42, 641
DpnI GATC 4 cut(s) 198, 282, 626, 659
DpnII GATC 4 cut(s) 196, 280, 624, 657
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 687
Eco47I GGWCC 3 cut(s) 449, 671, 757
Eco57I CTGAAG 1 cut(s) 121
Eco88I CYCGRG 1 cut(s) 394
EcoO109I RGGNCCY 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 518
EcoRII CCWGG 2 cut(s) 315, 451
EcoT14I CCWWGG 1 cut(s) 687
ErhI CCWWGG 1 cut(s) 687
FaeI CATG 6 cut(s) 19, 260, 376, 587, 691, 730
FaqI GGGAC 2 cut(s) 377, 651
FatI CATG 6 cut(s) 15, 256, 372, 583, 687, 726
Fnu4HI GCNGC 2 cut(s) 292, 474
FokI GGATG 2 cut(s) 338, 412
Fsp4HI GCNGC 2 cut(s) 292, 474
FspBI CTAG 3 cut(s) 218, 348, 663
GlaI GCGC 1 cut(s) 13
GluI GCNGC 2 cut(s) 292, 474
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 2 cut(s) 607, 686
HapII CCGG 2 cut(s) 234, 442
HhaI GCGC 1 cut(s) 14
Hin1II CATG 6 cut(s) 19, 260, 376, 587, 691, 730
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HinfI GANTC 3 cut(s) 412, 418, 750
HpaII CCGG 2 cut(s) 234, 442
HphI GGTGA 4 cut(s) 51, 114, 331, 388
Hpy166II GTNNAC 2 cut(s) 322, 700
Hpy188I TCNGA 8 cut(s) 140, 157, 185, 388, 417, 437, 736, 749
Hpy188III TCNNGA 6 cut(s) 218, 394, 425, 548, 563, 632
Hpy8I GTNNAC 2 cut(s) 322, 700
Hpy99I CGWCG 1 cut(s) 500
HpyAV CCTTC 5 cut(s) 135, 145, 240, 514, 662
HpyCH4III ACNGT 4 cut(s) 23, 302, 359, 579
HpyCH4IV ACGT 2 cut(s) 334, 498
HpyCH4V TGCA 7 cut(s) 34, 243, 460, 473, 485, 766, 792
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 2 cut(s) 42, 641
HpySE526I ACGT 2 cut(s) 334, 498
Hsp92II CATG 6 cut(s) 19, 260, 376, 587, 691, 730
HspAI GCGC 1 cut(s) 12
KpnI GGTACC 1 cut(s) 41
KroI GCCGGC 1 cut(s) 441
KroNI GCCGGC 1 cut(s) 443
Kzo9I GATC 4 cut(s) 196, 280, 624, 657
LmnI GCTCC 1 cut(s) 530
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 3 cut(s) 86, 148, 360
MaeI CTAG 3 cut(s) 218, 348, 663
MaeII ACGT 2 cut(s) 334, 498
MaeIII GTNAC 3 cut(s) 57, 259, 376
MalI GATC 4 cut(s) 198, 282, 626, 659
MboI GATC 4 cut(s) 196, 280, 624, 657
MboII GAAGA 5 cut(s) 59, 99, 102, 275, 413
MflI RGATCY 2 cut(s) 196, 624
MhlI GDGCHC 1 cut(s) 504
MluCI AATT 4 cut(s) 343, 518, 540, 596
MlyI GAGTC 1 cut(s) 759
MnlI CCTC 9 cut(s) 336, 390, 555, 581, 627, 628, 749, 755, 762
MroNI GCCGGC 1 cut(s) 441
MroXI GAANNNNTTC 1 cut(s) 134
MseI TTAA 3 cut(s) 113, 509, 590
MslI CAYNNNNRTG 1 cut(s) 387
MspI CCGG 2 cut(s) 234, 442
MspR9I CCNGG 2 cut(s) 317, 453
MvaI CCWGG 2 cut(s) 317, 453
MvnI CGCG 2 cut(s) 279, 755
MwoI GCNNNNNNNGC 1 cut(s) 482
NaeI GCCGGC 1 cut(s) 443
NcoI CCATGG 1 cut(s) 687
NdeII GATC 4 cut(s) 196, 280, 624, 657
NgoMIV GCCGGC 1 cut(s) 441
NlaIII CATG 6 cut(s) 19, 260, 376, 587, 691, 730
NlaIV GGNNCC 2 cut(s) 39, 615
NmuCI GTSAC 2 cut(s) 57, 376
NspI RCATGY 1 cut(s) 260
NspV TTCGAA 1 cut(s) 128
PciI ACATGT 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PdiI GCCGGC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 2 cut(s) 412, 418
PkrI GCNGC 2 cut(s) 293, 475
PleI GAGTC 1 cut(s) 758
PpsI GAGTC 1 cut(s) 758
PpuMI RGGWCCY 1 cut(s) 449
PscI ACATGT 1 cut(s) 256
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 315, 451
PspGI CCWGG 2 cut(s) 315, 451
PspN4I GGNNCC 2 cut(s) 39, 615
PspPI GGNCC 5 cut(s) 449, 605, 671, 684, 757
PspPPI RGGWCCY 1 cut(s) 449
PstI CTGCAG 1 cut(s) 794
PstNI CAGNNNCTG 1 cut(s) 528
PsuI RGATCY 2 cut(s) 196, 624
RsaI GTAC 2 cut(s) 20, 39
RsaNI GTAC 2 cut(s) 19, 38
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 3 cut(s) 113, 509, 590
SatI GCNGC 2 cut(s) 292, 474
Sau3AI GATC 4 cut(s) 196, 280, 624, 657
Sau96I GGNCC 5 cut(s) 449, 605, 671, 684, 757
SchI GAGTC 1 cut(s) 759
ScrFI CCNGG 2 cut(s) 317, 453
SduI GDGCHC 1 cut(s) 504
SfaNI GCATC 3 cut(s) 86, 148, 360
SfcI CTRYAG 1 cut(s) 790
SfuI TTCGAA 1 cut(s) 128
SinI GGWCC 3 cut(s) 449, 671, 757
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 2 cut(s) 162, 632
SmoI CTYRAG 2 cut(s) 162, 632
Sse9I AATT 4 cut(s) 343, 518, 540, 596
SsiI CCGC 4 cut(s) 108, 277, 291, 755
SspI AATATT 1 cut(s) 210
SspMI CTAG 3 cut(s) 218, 348, 663
StyD4I CCNGG 2 cut(s) 315, 451
StyI CCWWGG 1 cut(s) 687
TaaI ACNGT 4 cut(s) 23, 302, 359, 579
TaiI ACGT 2 cut(s) 337, 501
TaqI TCGA 4 cut(s) 70, 128, 426, 495
TasI AATT 4 cut(s) 343, 518, 540, 596
TatI WGTACW 1 cut(s) 18
TauI GCSGC 1 cut(s) 294
TfiI GAWTC 2 cut(s) 412, 418
Tru1I TTAA 3 cut(s) 113, 509, 590
Tru9I TTAA 3 cut(s) 113, 509, 590
TscAI CASTG 1 cut(s) 582
TseFI GTSAC 2 cut(s) 57, 376
TseI GCWGC 1 cut(s) 473
Tsp45I GTSAC 2 cut(s) 57, 376
TspRI CASTG 1 cut(s) 582
VpaK11BI GGWCC 3 cut(s) 449, 671, 757
XapI RAATTY 3 cut(s) 343, 518, 540
XbaI TCTAGA 1 cut(s) 217
XceI RCATGY 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 3 cut(s) 218, 348, 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.