pycom13g14110

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011130
Physical Location & Seq
Reverse (-)
10097226 .. 10098116
891 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g14110.1

Sequence Viewer

Length: 891 bp
ATGGACCGCCGCCCGCACGCCGACCATCTCGACGCCGCCCCGACGCAGCCCTACAAGGGCTACGAGCTCAGCGGCAAGATCATGCTCTGCGCCATCGTCATCCTCTTCTTCGTCGTCATCCTCATGCTCTGCCTCCACCTCTACGCGCGTTGGTACCTCCTGCGCCGCCCTCGCCCCCATGTCCGCCGCAATCGCCGGAACCGCCGCACCCATATTGTCTTTCACGAGGACGCGGCTGCCCTCTCCGCCGTCCCCACCAGCAGCCTCGACGCCTCCATCCTTAATTCCCTCCCTGTATTCGTTTACTCGTCCAAATCGAGTCAAATGGCGCCGTTTCGGCAGCAGCCCATTTTGGAGTGCGCCGTCTGCTTGTCGGAATTCGAGGACGACGAAACGGGGCGTTTGCTTCCCAAGTGTAAGCACAGCTTCCATACCGAGTGTATTGATATGTGGTTCCAATCTCACTCCACGTGTCCCCTCTGCCGGGCCATCGTGGAACCGGGCCCGGAGTCCGAAACCCGGGCCGACGTGGTTCTGAATGTCTGCGAACCGGACGAGCCGGGTCCGAGCTCCTATTTGTGCTCGGAGTGTTGTAATTCCGAGTCGGCGTCGCCTGGGGGGCGGAGAAAGCCGTCGAACATCGTGATTCCGAGAAGGAACGAGAGTTTTGGGAAGGGGGAGGGCTCGGGATGCGGCGAATCGCCGGCGGCTCAATCATTTAGGTCCCCGATGAGTCGGATGTTGTCGTTTAGGACAATGCTAAATAGGGAAAAACGGAACGGCGGCGTTTCGCCTTCGGGAGGAAACGGGGGGAGTTCCAGTTCGGTGGCGGGGTCGGATATCGAACTCGGTGGACGACTAGGGACCAGCGAGTGCACTCGGAGAGAGTAA

Protein Analysis

297

Amino Acids

32.68

Weight (kDa)

8.39

Isoelectric Point (pI)

81.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 119 - 162 1e-13 Ring finger domain
zf-RING_11 PF17123 119 - 148 4.1e-10 RING-like zinc finger
zf-rbx1 PF12678 119 - 162 1.2e-08 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 119 - 161 3.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 120 - 161 3.4e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 153
AccB1I GGYRCC 2 cut(s) 153, 328
AccII CGCG 3 cut(s) 146, 148, 233
AcsI RAATTY 1 cut(s) 377
AcvI CACGTG 1 cut(s) 471
AcyI GRCGYC 4 cut(s) 33, 270, 329, 608
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 5 cut(s) 56, 483, 484, 519, 800
AflIII ACRYGT 1 cut(s) 470
AjiI CACGTC 1 cut(s) 529
AjnI CCWGG 1 cut(s) 613
AloI GAACNNNNNNTCC 2 cut(s) 805, 837
AluBI AGCT 3 cut(s) 67, 426, 570
AluI AGCT 3 cut(s) 67, 426, 570
Alw21I GWGCWC 4 cut(s) 69, 572, 584, 878
Alw44I GTGCAC 1 cut(s) 874
Ama87I CYCGRG 2 cut(s) 519, 685
AoxI GGCC 3 cut(s) 486, 502, 522
ApaI GGGCCC 1 cut(s) 506
ApaLI GTGCAC 1 cut(s) 874
ApeKI GCWGC 5 cut(s) 46, 236, 261, 340, 343
ApoI RAATTY 1 cut(s) 377
Asp718I GGTACC 1 cut(s) 153
AspLEI GCGC 5 cut(s) 92, 148, 165, 331, 362
AspS9I GGNCC 8 cut(s) 4, 486, 502, 503, 522, 563, 723, 864
AsuC2I CCSGG 6 cut(s) 485, 501, 506, 520, 521, 561
AvaI CYCGRG 2 cut(s) 519, 685
AvaII GGWCC 4 cut(s) 4, 563, 723, 864
BaeGI GKGCMC 2 cut(s) 506, 878
BanI GGYRCC 2 cut(s) 153, 328
BanII GRGCYC 4 cut(s) 69, 506, 572, 686
BauI CACGAG 1 cut(s) 224
BbrPI CACGTG 1 cut(s) 471
Bbv12I GWGCWC 4 cut(s) 69, 572, 584, 878
BbvI GCAGC 5 cut(s) 58, 223, 273, 352, 355
BccI CCATC 4 cut(s) 33, 101, 284, 497
BceAI ACGGC 5 cut(s) 233, 316, 347, 616, 796
BciT130I CCWGG 1 cut(s) 615
BcnI CCSGG 6 cut(s) 485, 501, 506, 520, 521, 561
BfaI CTAG 1 cut(s) 860
BfoI RGCGCY 1 cut(s) 332
BglI GCCNNNNNGGC 2 cut(s) 337, 619
BlpI GCTNAGC 1 cut(s) 68
Bme1390I CCNGG 7 cut(s) 485, 501, 506, 520, 521, 561, 615
Bme18I GGWCC 4 cut(s) 4, 563, 723, 864
BmeT110I CYCGRG 2 cut(s) 519, 685
BmgBI CACGTC 1 cut(s) 529
BmgT120I GGNCC 8 cut(s) 4, 486, 502, 503, 522, 563, 723, 864
BmiI GGNNCC 9 cut(s) 155, 200, 330, 455, 498, 504, 564, 725, 865
BmrFI CCNGG 7 cut(s) 485, 501, 506, 520, 521, 561, 615
BmsI GCATC 1 cut(s) 680
Bpu1102I GCTNAGC 1 cut(s) 68
BpuMI CCSGG 6 cut(s) 485, 501, 506, 520, 521, 561
BsaAI YACGTR 1 cut(s) 471
BsaHI GRCGYC 4 cut(s) 33, 270, 329, 608
BsaJI CCNNGG 2 cut(s) 519, 614
BsaWI WCCGGW 1 cut(s) 550
BsaXI ACNNNNNCTCC 2 cut(s) 805, 835
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 483, 484, 519, 800
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 1 cut(s) 819
BseBI CCWGG 1 cut(s) 615
BseDI CCNNGG 2 cut(s) 519, 614
BseGI GGATG 5 cut(s) 99, 117, 276, 695, 744
BseLI CCNNNNNNNGG 5 cut(s) 56, 483, 484, 519, 800
BseMII CTCAG 1 cut(s) 82
BseNI ACTGG 1 cut(s) 819
BseSI GKGCMC 2 cut(s) 506, 878
BseXI GCAGC 5 cut(s) 58, 223, 273, 352, 355
Bsh1236I CGCG 3 cut(s) 146, 148, 233
BshFI GGCC 3 cut(s) 488, 504, 524
BshNI GGYRCC 2 cut(s) 153, 328
BsiHKAI GWGCWC 4 cut(s) 69, 572, 584, 878
BsiHKCI CYCGRG 2 cut(s) 519, 685
BsiSI CCGG 8 cut(s) 196, 484, 500, 506, 520, 551, 560, 704
BslFI GGGAC 4 cut(s) 236, 459, 709, 877
BslI CCNNNNNNNGG 5 cut(s) 56, 483, 484, 519, 800
BsmFI GGGAC 4 cut(s) 236, 459, 709, 877
BsnI GGCC 3 cut(s) 488, 504, 524
BsoBI CYCGRG 2 cut(s) 519, 685
Bsp120I GGGCCC 1 cut(s) 502
Bsp1286I GDGCHC 6 cut(s) 69, 506, 572, 584, 686, 878
Bsp143I GATC 1 cut(s) 78
Bsp1720I GCTNAGC 1 cut(s) 68
BspANI GGCC 3 cut(s) 488, 504, 524
BspCNI CTCAG 1 cut(s) 81
BspFNI CGCG 3 cut(s) 146, 148, 233
BspLI GGNNCC 9 cut(s) 155, 200, 330, 455, 498, 504, 564, 725, 865
BspT107I GGYRCC 2 cut(s) 153, 328
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 1 cut(s) 819
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 2 cut(s) 519, 614
BssMI GATC 1 cut(s) 78
BssNI GRCGYC 4 cut(s) 33, 270, 329, 608
BssSI CACGAG 1 cut(s) 224
Bst2BI CACGAG 1 cut(s) 224
Bst2UI CCWGG 1 cut(s) 615
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 4 cut(s) 33, 270, 329, 608
BstBAI YACGTR 1 cut(s) 471
BstC8I GCNNGC 3 cut(s) 14, 18, 705
BstDEI CTNAG 1 cut(s) 68
BstENI CCTNNNNNAGG 1 cut(s) 798
BstF5I GGATG 5 cut(s) 99, 117, 276, 695, 744
BstFNI CGCG 3 cut(s) 146, 148, 233
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 5 cut(s) 92, 148, 165, 331, 362
BstKTI GATC 1 cut(s) 81
BstMBI GATC 1 cut(s) 78
BstMWI GCNNNNNNNGC 9 cut(s) 171, 192, 201, 245, 337, 366, 619, 628, 690
BstNI CCWGG 1 cut(s) 615
BstSCI CCNGG 7 cut(s) 483, 499, 504, 518, 519, 559, 613
BstSLI GKGCMC 2 cut(s) 506, 878
BstUI CGCG 3 cut(s) 146, 148, 233
BstV1I GCAGC 5 cut(s) 58, 223, 273, 352, 355
BstXI CCANNNNNNTGG 1 cut(s) 826
BsuRI GGCC 3 cut(s) 488, 504, 524
BtrI CACGTC 1 cut(s) 529
BtsCI GGATG 5 cut(s) 99, 117, 276, 695, 744
Cac8I GCNNGC 3 cut(s) 14, 18, 705
CfoI GCGC 5 cut(s) 92, 148, 165, 331, 362
Cfr10I RCCGGY 1 cut(s) 703
Cfr13I GGNCC 8 cut(s) 4, 486, 502, 503, 522, 563, 723, 864
Cfr9I CCCGGG 1 cut(s) 519
CseI GACGC 5 cut(s) 41, 52, 239, 278, 597
Csp6I GTAC 1 cut(s) 154
CviAII CATG 3 cut(s) 82, 124, 179
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 1 cut(s) 68
DinI GGCGCC 1 cut(s) 330
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 3 cut(s) 173, 235, 637
Ecl136II GAGCTC 2 cut(s) 67, 570
Eco24I GRGCYC 4 cut(s) 69, 506, 572, 686
Eco32I GATATC 1 cut(s) 841
Eco47I GGWCC 4 cut(s) 4, 563, 723, 864
Eco53kI GAGCTC 2 cut(s) 67, 570
Eco72I CACGTG 1 cut(s) 471
Eco88I CYCGRG 2 cut(s) 519, 685
EcoICRI GAGCTC 2 cut(s) 67, 570
EcoNI CCTNNNNNAGG 1 cut(s) 798
EcoO109I RGGNCCY 1 cut(s) 723
EcoRI GAATTC 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 613
EcoRV GATATC 1 cut(s) 841
EcoT38I GRGCYC 4 cut(s) 69, 506, 572, 686
EgeI GGCGCC 1 cut(s) 330
EheI GGCGCC 1 cut(s) 330
FaeI CATG 3 cut(s) 85, 127, 182
FaiI YATR 6 cut(s) 83, 125, 180, 213, 432, 449
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 4 cut(s) 236, 459, 709, 877
FatI CATG 3 cut(s) 81, 123, 178
FauI CCCGC 2 cut(s) 21, 823
FokI GGATG 5 cut(s) 86, 104, 263, 702, 751
FriOI GRGCYC 4 cut(s) 69, 506, 572, 686
FspBI CTAG 1 cut(s) 860
GlaI GCGC 5 cut(s) 91, 147, 164, 330, 361
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 3 cut(s) 488, 504, 524
HapII CCGG 8 cut(s) 196, 484, 500, 506, 520, 551, 560, 704
HgaI GACGC 5 cut(s) 41, 52, 239, 278, 597
HhaI GCGC 5 cut(s) 92, 148, 165, 331, 362
Hin1I GRCGYC 4 cut(s) 33, 270, 329, 608
Hin1II CATG 3 cut(s) 85, 127, 182
Hin6I GCGC 5 cut(s) 90, 146, 163, 329, 360
HinP1I GCGC 5 cut(s) 90, 146, 163, 329, 360
HinfI GANTC 6 cut(s) 319, 509, 602, 646, 698, 733
HpaII CCGG 8 cut(s) 196, 484, 500, 506, 520, 551, 560, 704
Hpy166II GTNNAC 3 cut(s) 304, 854, 876
Hpy188III TCNNGA 5 cut(s) 29, 224, 643, 687, 798
Hpy8I GTNNAC 3 cut(s) 304, 854, 876
Hpy99I CGWCG 8 cut(s) 35, 46, 116, 272, 392, 530, 613, 637
HpyAV CCTTC 3 cut(s) 648, 667, 804
HpyCH4IV ACGT 2 cut(s) 470, 528
HpyCH4V TGCA 1 cut(s) 876
HpyF10VI GCNNNNNNNGC 9 cut(s) 171, 192, 201, 245, 337, 366, 619, 628, 690
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 2 cut(s) 470, 528
Hsp92I GRCGYC 4 cut(s) 33, 270, 329, 608
Hsp92II CATG 3 cut(s) 85, 127, 182
HspAI GCGC 5 cut(s) 90, 146, 163, 329, 360
KasI GGCGCC 1 cut(s) 328
KpnI GGTACC 1 cut(s) 157
KroI GCCGGC 1 cut(s) 703
KroNI GCCGGC 1 cut(s) 705
Kzo9I GATC 1 cut(s) 78
LmnI GCTCC 1 cut(s) 575
Lsp1109I GCAGC 5 cut(s) 58, 223, 273, 352, 355
LweI GCATC 1 cut(s) 680
MaeI CTAG 1 cut(s) 860
MaeII ACGT 2 cut(s) 470, 528
MalI GATC 1 cut(s) 80
MboI GATC 1 cut(s) 78
MboII GAAGA 2 cut(s) 97, 100
MhlI GDGCHC 6 cut(s) 69, 506, 572, 584, 686, 878
MluCI AATT 3 cut(s) 283, 377, 595
Mly113I GGCGCC 1 cut(s) 329
MlyI GAGTC 4 cut(s) 328, 518, 611, 742
MmeI TCCRAC 3 cut(s) 354, 716, 816
MreI CGCCGGCG 1 cut(s) 703
MroNI GCCGGC 1 cut(s) 703
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 72
MspI CCGG 8 cut(s) 196, 484, 500, 506, 520, 551, 560, 704
MspR9I CCNGG 7 cut(s) 485, 501, 506, 520, 521, 561, 615
MvaI CCWGG 1 cut(s) 615
MvnI CGCG 3 cut(s) 146, 148, 233
MwoI GCNNNNNNNGC 9 cut(s) 171, 192, 201, 245, 337, 366, 619, 628, 690
NaeI GCCGGC 1 cut(s) 705
NarI GGCGCC 1 cut(s) 329
NciI CCSGG 6 cut(s) 485, 501, 506, 520, 521, 561
NdeII GATC 1 cut(s) 78
NgoMIV GCCGGC 1 cut(s) 703
NlaIII CATG 3 cut(s) 85, 127, 182
NlaIV GGNNCC 9 cut(s) 155, 200, 330, 455, 498, 504, 564, 725, 865
PcsI WCGNNNNNNNCGW 2 cut(s) 314, 387
PdiI GCCGGC 1 cut(s) 705
PfeI GAWTC 2 cut(s) 646, 698
PleI GAGTC 4 cut(s) 327, 517, 610, 741
PluTI GGCGCC 1 cut(s) 332
PmaCI CACGTG 1 cut(s) 471
PmlI CACGTG 1 cut(s) 471
PpsI GAGTC 4 cut(s) 327, 517, 610, 741
Ppu21I YACGTR 1 cut(s) 471
PpuMI RGGWCCY 1 cut(s) 723
Psp124BI GAGCTC 2 cut(s) 69, 572
Psp5II RGGWCCY 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 613
PspCI CACGTG 1 cut(s) 471
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 9 cut(s) 155, 200, 330, 455, 498, 504, 564, 725, 865
PspOMI GGGCCC 1 cut(s) 502
PspPI GGNCC 8 cut(s) 4, 486, 502, 503, 522, 563, 723, 864
PspPPI RGGWCCY 1 cut(s) 723
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 122
SacI GAGCTC 2 cut(s) 69, 572
SaqAI TTAA 1 cut(s) 282
Sau3AI GATC 1 cut(s) 78
Sau96I GGNCC 8 cut(s) 4, 486, 502, 503, 522, 563, 723, 864
SchI GAGTC 4 cut(s) 328, 518, 611, 742
ScrFI CCNGG 7 cut(s) 485, 501, 506, 520, 521, 561, 615
SduI GDGCHC 6 cut(s) 69, 506, 572, 584, 686, 878
SetI ASST 8 cut(s) 69, 141, 159, 428, 473, 531, 572, 725
SfaNI GCATC 1 cut(s) 680
SfoI GGCGCC 1 cut(s) 330
SgrAI CRCCGGYG 1 cut(s) 703
SinI GGWCC 4 cut(s) 4, 563, 723, 864
SmaI CCCGGG 1 cut(s) 521
SmiMI CAYNNNNRTG 1 cut(s) 122
Sse9I AATT 3 cut(s) 283, 377, 595
SspDI GGCGCC 1 cut(s) 328
SspMI CTAG 1 cut(s) 860
SstI GAGCTC 2 cut(s) 69, 572
StyD4I CCNGG 7 cut(s) 483, 499, 504, 518, 519, 559, 613
TaiI ACGT 2 cut(s) 473, 531
TaqI TCGA 6 cut(s) 30, 267, 317, 381, 635, 843
TasI AATT 3 cut(s) 283, 377, 595
TfiI GAWTC 2 cut(s) 646, 698
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TseI GCWGC 5 cut(s) 46, 236, 261, 340, 343
TspGWI ACGGA 1 cut(s) 790
TspMI CCCGGG 1 cut(s) 519
VneI GTGCAC 1 cut(s) 874
VpaK11BI GGWCC 4 cut(s) 4, 563, 723, 864
XagI CCTNNNNNAGG 1 cut(s) 798
XapI RAATTY 1 cut(s) 377
XmaI CCCGGG 1 cut(s) 519
XspI CTAG 1 cut(s) 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.