Rroxscaffold_5G00365520

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011130
Physical Location & Seq
Forward (+)
47104645 .. 47105514
870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00365520.1

Sequence Viewer

Length: 870 bp
ATGTCTGGCTTCAACGACTTACCCCACCGCCCGGATTTGTCTACATACTCCACTCCCGTGCAGGAGCCCTACAAGGGCTACGCGCTCAGCGGCAAGATTATGCTGAGCGCCATCGTCATCCTCTTCTTCGTCGTCATTCTAATGGTGTGTCTCCACCTCTACGCGCGCTGGTACCTCCTCCGAGCGCGCCGCCGCAACCTCCGCAGAAACCGCCGCAACCGCCGCAACTTGATCTTCCACGAGGACGAGGCCACAGCCGCCGTCTCGGCCGCCATGCGCGGCCTCGACGAGTCGGTGCTCAAGTCGCTGCCGGTGTTCGTCTACGACTCGAAGACCCACCCGGAGCCGATTCTGGAGTGCGCCGTGTGCTTGTCGGAGTTCGAAGAGAACGAAACGGGTCGGGTTCTCCCGAAATGTAAGCACAGCTTCCATATCGAGTGTATCGACATGTGGTTCCACTCGCACTCCACCTGCCCGCTCTGTCGGTGCGCGGTGGAGCCCCAACCCGAGTCCGAGAACCCGGGCGATGTGGTGTTGAATGTGTGCGAACCCGAGAGTAACGAACCGGGTTCGAGCTCCGGGTTGTGCTCCGAGTGTTCCGGGTCGAGGTCGTTACGGCAGACGTCGTCGTTTGGGCCGAGAAGGAAACCGATGGATATCGCGGTGCCGAGTCGGCACGAGAGCTTTGGGAGGTCGGAGGCGGAGTCGCCGTCGGGTCAGGCGTTTCGGTCGCCGATGAGTCGGATGCTGTCGTTTAAACGGATGCTGAGCAGAGACAAAAGAAACGGCGGCGTTTCGCCTTCCGGGAATGCGAGTTGTAGCTCGGTCGCCGAGTTGGATATTGAGCGAGGTCGAGAAGGGGCTGAGTGA

Protein Analysis

289

Amino Acids

32.34

Weight (kDa)

7.98

Isoelectric Point (pI)

81.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 119 - 162 6.9e-13 Ring finger domain
zf-RING_11 PF17123 119 - 148 4.3e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 119 - 161 3e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 120 - 162 8.7e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 120 - 161 2.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 479
AatII GACGTC 1 cut(s) 626
Acc36I ACCTGC 1 cut(s) 479
Acc65I GGTACC 1 cut(s) 171
AccB1I GGYRCC 2 cut(s) 171, 664
AccBSI CCGCTC 1 cut(s) 478
AccI GTMKAC 2 cut(s) 41, 321
AccII CGCG 7 cut(s) 83, 164, 166, 187, 279, 491, 662
AcoI YGGCCR 1 cut(s) 267
AcyI GRCGYC 1 cut(s) 623
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 3 cut(s) 31, 74, 606
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 2 cut(s) 13, 538
AleI CACNNNNGTG 1 cut(s) 56
AluBI AGCT 4 cut(s) 426, 576, 684, 822
AluI AGCT 4 cut(s) 426, 576, 684, 822
Alw21I GWGCWC 3 cut(s) 300, 578, 590
Alw26I GTCTC 3 cut(s) 155, 268, 768
Ama87I CYCGRG 3 cut(s) 506, 520, 551
AoxI GGCC 4 cut(s) 249, 267, 280, 635
ApeKI GCWGC 1 cut(s) 307
Asp718I GGTACC 1 cut(s) 171
AspLEI GCGC 9 cut(s) 85, 110, 166, 168, 187, 189, 279, 362, 491
AspS9I GGNCC 1 cut(s) 635
AsuC2I CCSGG 8 cut(s) 32, 341, 521, 522, 567, 580, 601, 805
AsuII TTCGAA 1 cut(s) 381
AvaI CYCGRG 3 cut(s) 506, 520, 551
BanI GGYRCC 2 cut(s) 171, 664
BanII GRGCYC 3 cut(s) 69, 501, 578
BauI CACGAG 2 cut(s) 239, 677
BbsI GAAGAC 1 cut(s) 338
Bbv12I GWGCWC 3 cut(s) 300, 578, 590
BbvI GCAGC 1 cut(s) 294
BccI CCATC 2 cut(s) 119, 646
BceAI ACGGC 5 cut(s) 245, 347, 632, 694, 802
BcnI CCSGG 8 cut(s) 32, 341, 521, 522, 567, 580, 601, 805
BcoDI GTCTC 3 cut(s) 155, 268, 768
BfoI RGCGCY 1 cut(s) 111
BfuAI ACCTGC 1 cut(s) 479
BglI GCCNNNNNGGC 2 cut(s) 266, 673
BlpI GCTNAGC 3 cut(s) 86, 104, 767
Bme1390I CCNGG 8 cut(s) 32, 341, 521, 522, 567, 580, 601, 805
BmeT110I CYCGRG 3 cut(s) 506, 520, 551
BmgT120I GGNCC 1 cut(s) 635
BmiI GGNNCC 6 cut(s) 66, 173, 345, 455, 498, 666
BmrFI CCNGG 8 cut(s) 32, 341, 521, 522, 567, 580, 601, 805
BmsI GCATC 2 cut(s) 735, 753
BpiI GAAGAC 1 cut(s) 338
BpmI CTGGAG 1 cut(s) 374
Bpu1102I GCTNAGC 3 cut(s) 86, 104, 767
Bpu14I TTCGAA 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 8 cut(s) 32, 341, 521, 522, 567, 580, 601, 805
BsaBI GATNNNNATC 1 cut(s) 656
BsaHI GRCGYC 1 cut(s) 623
BsaJI CCNNGG 1 cut(s) 520
BsaXI ACNNNNNCTCC 6 cut(s) 449, 479, 689, 695, 719, 725
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 74, 606
Bse118I RCCGGY 1 cut(s) 310
Bse8I GATNNNNATC 1 cut(s) 656
BseDI CCNNGG 1 cut(s) 520
BseGI GGATG 3 cut(s) 117, 750, 768
BseJI GATNNNNATC 1 cut(s) 656
BseLI CCNNNNNNNGG 3 cut(s) 31, 74, 606
BseMII CTCAG 4 cut(s) 95, 100, 758, 855
BsePI GCGCGC 2 cut(s) 164, 185
BseRI GAGGAG 1 cut(s) 167
BseX3I CGGCCG 1 cut(s) 267
BseXI GCAGC 1 cut(s) 294
BsgI GTGCAG 1 cut(s) 80
Bsh1236I CGCG 7 cut(s) 83, 164, 166, 187, 279, 491, 662
Bsh1285I CGRYCG 3 cut(s) 270, 731, 828
BshFI GGCC 4 cut(s) 251, 269, 282, 637
BshNI GGYRCC 2 cut(s) 171, 664
BsiEI CGRYCG 3 cut(s) 270, 731, 828
BsiHKAI GWGCWC 3 cut(s) 300, 578, 590
BsiHKCI CYCGRG 3 cut(s) 506, 520, 551
BsiSI CCGG 8 cut(s) 32, 311, 341, 521, 566, 579, 600, 804
BslI CCNNNNNNNGG 3 cut(s) 31, 74, 606
BsmAI GTCTC 3 cut(s) 155, 268, 768
BsmBI CGTCTC 1 cut(s) 268
BsmI GAATGC 1 cut(s) 814
BsnI GGCC 4 cut(s) 251, 269, 282, 637
BsoBI CYCGRG 3 cut(s) 506, 520, 551
Bsp119I TTCGAA 1 cut(s) 381
Bsp1286I GDGCHC 5 cut(s) 69, 300, 501, 578, 590
Bsp143I GATC 1 cut(s) 231
Bsp1720I GCTNAGC 3 cut(s) 86, 104, 767
BspANI GGCC 4 cut(s) 251, 269, 282, 637
BspCNI CTCAG 4 cut(s) 96, 99, 759, 856
BspFNI CGCG 7 cut(s) 83, 164, 166, 187, 279, 491, 662
BspLI GGNNCC 6 cut(s) 66, 173, 345, 455, 498, 666
BspMI ACCTGC 1 cut(s) 479
BspT104I TTCGAA 1 cut(s) 381
BspT107I GGYRCC 2 cut(s) 171, 664
BsrBI CCGCTC 1 cut(s) 478
BsrFI RCCGGY 1 cut(s) 310
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 1 cut(s) 520
BssHII GCGCGC 2 cut(s) 164, 185
BssMI GATC 1 cut(s) 231
BssNI GRCGYC 1 cut(s) 623
BssSI CACGAG 2 cut(s) 239, 677
Bst2BI CACGAG 2 cut(s) 239, 677
Bst6I CTCTTC 2 cut(s) 128, 378
BstACI GRCGYC 1 cut(s) 623
BstBI TTCGAA 1 cut(s) 381
BstC8I GCNNGC 3 cut(s) 166, 187, 476
BstDEI CTNAG 4 cut(s) 86, 104, 767, 864
BstF5I GGATG 3 cut(s) 117, 750, 768
BstFNI CGCG 7 cut(s) 83, 164, 166, 187, 279, 491, 662
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 9 cut(s) 85, 110, 166, 168, 187, 189, 279, 362, 491
BstKTI GATC 1 cut(s) 234
BstMAI GTCTC 3 cut(s) 155, 268, 768
BstMBI GATC 1 cut(s) 231
BstMCI CGRYCG 3 cut(s) 270, 731, 828
BstMWI GCNNNNNNNGC 9 cut(s) 201, 210, 219, 222, 257, 266, 304, 366, 673
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 8 cut(s) 30, 339, 519, 520, 565, 578, 599, 803
BstUI CGCG 7 cut(s) 83, 164, 166, 187, 279, 491, 662
BstV1I GCAGC 1 cut(s) 294
BstV2I GAAGAC 1 cut(s) 338
BstZI CGGCCG 1 cut(s) 267
BsuRI GGCC 4 cut(s) 251, 269, 282, 637
BtgZI GCGATG 1 cut(s) 540
BtsCI GGATG 3 cut(s) 117, 750, 768
BveI ACCTGC 1 cut(s) 479
Cac8I GCNNGC 3 cut(s) 166, 187, 476
CfoI GCGC 9 cut(s) 85, 110, 166, 168, 187, 189, 279, 362, 491
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 1 cut(s) 635
Cfr9I CCCGGG 1 cut(s) 520
Csp6I GTAC 1 cut(s) 172
CviAII CATG 2 cut(s) 274, 448
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 4 cut(s) 86, 104, 767, 864
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
DraI TTTAAA 1 cut(s) 757
EaeI YGGCCR 1 cut(s) 267
EagI CGGCCG 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 128, 378
EarI CTCTTC 2 cut(s) 128, 378
EciI GGCGGA 1 cut(s) 716
Ecl136II GAGCTC 1 cut(s) 576
EclXI CGGCCG 1 cut(s) 267
Eco24I GRGCYC 3 cut(s) 69, 501, 578
Eco32I GATATC 1 cut(s) 658
Eco52I CGGCCG 1 cut(s) 267
Eco53kI GAGCTC 1 cut(s) 576
Eco88I CYCGRG 3 cut(s) 506, 520, 551
EcoICRI GAGCTC 1 cut(s) 576
EcoRV GATATC 1 cut(s) 658
EcoT38I GRGCYC 3 cut(s) 69, 501, 578
Esp3I CGTCTC 1 cut(s) 268
FaeI CATG 2 cut(s) 277, 451
FaiI YATR 5 cut(s) 46, 101, 275, 432, 449
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FatI CATG 2 cut(s) 273, 447
FauI CCCGC 1 cut(s) 483
FblI GTMKAC 2 cut(s) 41, 321
FokI GGATG 3 cut(s) 104, 757, 775
FriOI GRGCYC 3 cut(s) 69, 501, 578
GlaI GCGC 9 cut(s) 84, 109, 165, 167, 186, 188, 278, 361, 490
GsuI CTGGAG 1 cut(s) 374
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 4 cut(s) 251, 269, 282, 637
HapII CCGG 8 cut(s) 32, 311, 341, 521, 566, 579, 600, 804
HhaI GCGC 9 cut(s) 85, 110, 166, 168, 187, 189, 279, 362, 491
Hin1I GRCGYC 1 cut(s) 623
Hin1II CATG 2 cut(s) 277, 451
Hin6I GCGC 9 cut(s) 83, 108, 164, 166, 185, 187, 277, 360, 489
HinP1I GCGC 9 cut(s) 83, 108, 164, 166, 185, 187, 277, 360, 489
HinfI GANTC 7 cut(s) 290, 326, 349, 509, 670, 704, 739
HpaII CCGG 8 cut(s) 32, 311, 341, 521, 566, 579, 600, 804
Hpy166II GTNNAC 2 cut(s) 42, 322
Hpy188I TCNGA 6 cut(s) 182, 376, 514, 592, 697, 744
Hpy188III TCNNGA 3 cut(s) 353, 409, 854
Hpy8I GTNNAC 2 cut(s) 42, 322
Hpy99I CGWCG 5 cut(s) 134, 290, 628, 631, 715
HpyAV CCTTC 3 cut(s) 636, 810, 851
HpyCH4IV ACGT 1 cut(s) 623
HpyCH4V TGCA 1 cut(s) 61
HpyF10VI GCNNNNNNNGC 9 cut(s) 201, 210, 219, 222, 257, 266, 304, 366, 673
HpyF3I CTNAG 4 cut(s) 86, 104, 767, 864
HpySE526I ACGT 1 cut(s) 623
Hsp92I GRCGYC 1 cut(s) 623
Hsp92II CATG 2 cut(s) 277, 451
HspAI GCGC 9 cut(s) 83, 108, 164, 166, 185, 187, 277, 360, 489
KpnI GGTACC 1 cut(s) 175
Kzo9I GATC 1 cut(s) 231
LmnI GCTCC 5 cut(s) 64, 343, 496, 581, 593
Lsp1109I GCAGC 1 cut(s) 294
LweI GCATC 2 cut(s) 735, 753
MaeII ACGT 1 cut(s) 623
MaeIII GTNAC 2 cut(s) 557, 612
MalI GATC 1 cut(s) 233
MbiI CCGCTC 1 cut(s) 478
MboI GATC 1 cut(s) 231
MboII GAAGA 5 cut(s) 115, 118, 226, 343, 395
MhlI GDGCHC 5 cut(s) 69, 300, 501, 578, 590
MlyI GAGTC 6 cut(s) 299, 320, 518, 679, 713, 748
MmeI TCCRAC 4 cut(s) 354, 675, 722, 816
MseI TTAA 1 cut(s) 756
MslI CAYNNNNRTG 2 cut(s) 56, 140
MspA1I CMGCKG 1 cut(s) 90
MspI CCGG 8 cut(s) 32, 311, 341, 521, 566, 579, 600, 804
MspR9I CCNGG 8 cut(s) 32, 341, 521, 522, 567, 580, 601, 805
MssI GTTTAAAC 1 cut(s) 757
Mva1269I GAATGC 1 cut(s) 814
MvnI CGCG 7 cut(s) 83, 164, 166, 187, 279, 491, 662
MwoI GCNNNNNNNGC 9 cut(s) 201, 210, 219, 222, 257, 266, 304, 366, 673
NciI CCSGG 8 cut(s) 32, 341, 521, 522, 567, 580, 601, 805
NdeII GATC 1 cut(s) 231
NlaIII CATG 2 cut(s) 277, 451
NlaIV GGNNCC 6 cut(s) 66, 173, 345, 455, 498, 666
NmeAIII GCCGAG 4 cut(s) 245, 663, 693, 856
NspI RCATGY 1 cut(s) 451
NspV TTCGAA 1 cut(s) 381
OliI CACNNNNGTG 1 cut(s) 56
PaqCI CACCTGC 1 cut(s) 479
PauI GCGCGC 2 cut(s) 164, 185
PciI ACATGT 1 cut(s) 447
PcsI WCGNNNNNNNCGW 4 cut(s) 387, 441, 635, 719
PctI GAATGC 1 cut(s) 814
PfeI GAWTC 1 cut(s) 349
PflFI GACNNNGTC 1 cut(s) 625
PfoI TCCNGGA 1 cut(s) 803
PleI GAGTC 6 cut(s) 298, 320, 517, 678, 712, 747
PmeI GTTTAAAC 1 cut(s) 757
PpsI GAGTC 6 cut(s) 298, 320, 517, 678, 712, 747
PscI ACATGT 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 578
PspN4I GGNNCC 6 cut(s) 66, 173, 345, 455, 498, 666
PspPI GGNCC 1 cut(s) 635
PsyI GACNNNGTC 1 cut(s) 625
PteI GCGCGC 2 cut(s) 164, 185
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 2 cut(s) 56, 140
SacI GAGCTC 1 cut(s) 578
SaqAI TTAA 1 cut(s) 756
Sau3AI GATC 1 cut(s) 231
Sau96I GGNCC 1 cut(s) 635
SchI GAGTC 6 cut(s) 299, 320, 518, 679, 713, 748
ScrFI CCNGG 8 cut(s) 32, 341, 521, 522, 567, 580, 601, 805
SduI GDGCHC 5 cut(s) 69, 300, 501, 578, 590
SfaNI GCATC 2 cut(s) 735, 753
SfuI TTCGAA 1 cut(s) 381
SmaI CCCGGG 1 cut(s) 522
SmiMI CAYNNNNRTG 2 cut(s) 56, 140
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
SstI GAGCTC 1 cut(s) 578
StyD4I CCNGG 8 cut(s) 30, 339, 519, 520, 565, 578, 599, 803
TaiI ACGT 1 cut(s) 626
TaqI TCGA 8 cut(s) 285, 329, 381, 435, 444, 572, 605, 853
TaqII GACCGA 2 cut(s) 717, 814
TauI GCSGC 9 cut(s) 93, 192, 195, 216, 225, 260, 272, 282, 792
TfiI GAWTC 1 cut(s) 349
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TseI GCWGC 1 cut(s) 307
TspGWI ACGGA 1 cut(s) 775
TspMI CCCGGG 1 cut(s) 520
Tth111I GACNNNGTC 1 cut(s) 625
XceI RCATGY 1 cut(s) 451
XmaI CCCGGG 1 cut(s) 520
XmiI GTMKAC 2 cut(s) 41, 321
ZraI GACGTC 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.