Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0005307.1

Orthogroup ID

OG0011130

Physical Location & Seq

Genomic Coordinates Forward (+)

17142 .. 19289

Gene Structure 2148 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0005307.1_g000003.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1218 bp

atgagtgttcgggtttggctcctttccgtgcatggagtcgtttgtcctctcaatggcggccagttgaggcgcagatcgagttgtgcggcggctgcggtggttgttcgtagtgagacatacggcggtggccaccagacatgcagcagtggtgcggactacgtgttgcacaggcaaattcccactagcagacaagaattcggtgagtccaagtctccaactgagagagaactagacaggaaactagtaatttcgacccgagtaagggccagggaagtaatttcccctcctccgcgtcaaccctgcggaagccgcagaggttctaagttcgagtctcccatgactatgtccggcttgtctagcttcaacgatgtccgcccggatgtgtctacacactccactcccccgtcggagccctacaagggctacgctctcagcggcaagattatgctgagcgccatcgtcatcctcttcttcgtcgtcattctaatggtgtgtctccacctctacgcgcgatggtacctcctccgcgcccgccgccgcaacctccgcagaaaccgccgcaaccgccgcaacaacttgatcttccacgaggacgaggccgccgccgccgccccggccgccatgcgcggcctcgacgagtcggtgctcaagtcgctgccggtgttcgtctacgactcgaagacccacccggagccgattctggagtgcgccgtgtgcttgtcggagttcgaagagaacgaaacgggtcgggttctcccgaaatgtaagcacagcttccatatcgagtgtatcgacatgtggttccactcgcactccacttgcccgctctgtcggtgcgcggtggagccccaacccgagtccgagaacccgggagatgtggtgttgaatgtgtgcgaacccgagagtaacgaaccgggttcgagctccgggttgtgctccgagtgttccgggtcgaggtcgttccggcagacgtcgtcgtttgggccgagaaggaaaccgatggatatcgcggtgccgagtcggcacgagagctttgggaggtcggaggcggagtcgccgtcgggtcaggcgtttcggtcgccgatgagtcggatgctgtcgtttaaacggatgctgagcagagacaaaagaaacggcggcgtttcgccttccgggaatgccagttgtagctccgtggccgagttggatattgagcgaggacgagaaggggctgagtga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010498 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0022622 GO:0030163 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0042221 GO:0042493 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044464 GO:0048364 GO:0048731 GO:0048856 GO:0050896 GO:0051301 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071944 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

405

Amino Acids

44.92

Weight (kDa)

9.02

Isoelectric Point (pI)

80.34

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011130)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16720
fragaria_vesca	FvH4_4g18630
malus_domestica	MD13G1165000.v1.1 MD16G1164700.v1.1
prunus_persica	Prupe.1G124000_v2.0.a1
pyrus_communis	pycom13g14110 pycom16g13940
rosa_chinensis	RchiOBHm_Chr4g0423331
rosa_laevigata	RLG00000007568
rosa_multiflora	Rmu_co8269179.1_g000001 Rmu_sc0001290.1_g000014 Rmu_sc0001290.1_g000015 Rmu_sc0005307.1_g000003
rosa_roxburghii	Rroxscaffold_5G00365520
rosa_rugosa	Rorug04G0186600
rosa_samantha	Rh4AG245800 Rh4BG251200 Rh4CG259600 Rh4DG246100
rosa_wichuraiana	Rw4G021120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	974
Acc65I	GGTACC	1 cut(s)	516
AccB1I	GGYRCC	2 cut(s)	516, 1012
AccBSI	CCGCTC	1 cut(s)	826
AccI	GTMKAC	2 cut(s)	386, 669
AccII	CGCG	7 cut(s)	292, 509, 511, 528, 627, 839, 1010
AcoI	YGGCCR	4 cut(s)	58, 127, 615, 1176
AcsI	RAATTY	2 cut(s)	174, 194
AcyI	GRCGYC	1 cut(s)	971
AfaI	GTAC	1 cut(s)	518
AfiI	CCNNNNNNNGG	5 cut(s)	53, 261, 262, 419, 954
AflIII	ACRYGT	2 cut(s)	159, 795
AgsI	TTSAA	2 cut(s)	364, 886
AhlI	ACTAGT	1 cut(s)	241
AjnI	CCWGG	1 cut(s)	266
AluBI	AGCT	5 cut(s)	360, 774, 924, 1032, 1170
AluI	AGCT	5 cut(s)	360, 774, 924, 1032, 1170
Alw21I	GWGCWC	3 cut(s)	648, 926, 938
Alw26I	GTCTC	5 cut(s)	107, 216, 336, 500, 1116
Ama87I	CYCGRG	4 cut(s)	255, 854, 868, 899
AoxI	GGCC	8 cut(s)	58, 127, 264, 597, 615, 628, 983, 1176
ApeKI	GCWGC	3 cut(s)	92, 141, 655
ApoI	RAATTY	2 cut(s)	174, 194
Asp718I	GGTACC	1 cut(s)	516
AspLEI	GCGC	7 cut(s)	72, 455, 511, 530, 627, 710, 839
AspS9I	GGNCC	2 cut(s)	264, 983
AsuC2I	CCSGG	9 cut(s)	377, 614, 689, 869, 870, 915, 928, 949, 1153
AsuHPI	GGTGA	1 cut(s)	212
AsuII	TTCGAA	1 cut(s)	729
AvaI	CYCGRG	4 cut(s)	255, 854, 868, 899
BalI	TGGCCA	1 cut(s)	129
BanI	GGYRCC	2 cut(s)	516, 1012
BanII	GRGCYC	3 cut(s)	414, 849, 926
BauI	CACGAG	2 cut(s)	587, 1025
BbsI	GAAGAC	1 cut(s)	686
Bbv12I	GWGCWC	3 cut(s)	648, 926, 938
BbvI	GCAGC	3 cut(s)	79, 153, 642
BccI	CCATC	3 cut(s)	464, 507, 994
BceAI	ACGGC	4 cut(s)	136, 695, 1042, 1150
BciT130I	CCWGG	1 cut(s)	268
BcnI	CCSGG	9 cut(s)	377, 614, 689, 869, 870, 915, 928, 949, 1153
BcoDI	GTCTC	5 cut(s)	107, 216, 336, 500, 1116
BcuI	ACTAGT	1 cut(s)	241
BfaI	CTAG	4 cut(s)	183, 230, 242, 357
BfoI	RGCGCY	1 cut(s)	456
BglI	GCCNNNNNGGC	2 cut(s)	614, 1021
BlpI	GCTNAGC	2 cut(s)	449, 1115
BmeT110I	CYCGRG	4 cut(s)	255, 854, 868, 899
BmgT120I	GGNCC	2 cut(s)	264, 983
BmiI	GGNNCC	7 cut(s)	20, 411, 518, 693, 803, 846, 1014
BmsI	GCATC	2 cut(s)	1083, 1101
BpiI	GAAGAC	1 cut(s)	686
BpmI	CTGGAG	1 cut(s)	722
Bpu1102I	GCTNAGC	2 cut(s)	449, 1115
Bpu14I	TTCGAA	1 cut(s)	729
BpuEI	CTTGAG	1 cut(s)	632
BpuMI	CCSGG	9 cut(s)	377, 614, 689, 869, 870, 915, 928, 949, 1153
BsaAI	YACGTR	1 cut(s)	160
BsaBI	GATNNNNATC	1 cut(s)	1004
BsaHI	GRCGYC	1 cut(s)	971
BsaJI	CCNNGG	4 cut(s)	267, 612, 868, 1173
BsaXI	ACNNNNNCTCC	8 cut(s)	797, 827, 864, 894, 1037, 1043, 1067, 1073
Bsc4I	CCNNNNNNNGG	5 cut(s)	53, 261, 262, 419, 954
Bse118I	RCCGGY	1 cut(s)	658
Bse1I	ACTGG	2 cut(s)	61, 1161
Bse8I	GATNNNNATC	1 cut(s)	1004
BseBI	CCWGG	1 cut(s)	268
BseDI	CCNNGG	4 cut(s)	267, 612, 868, 1173
BseGI	GGATG	4 cut(s)	385, 462, 1098, 1116
BseJI	GATNNNNATC	1 cut(s)	1004
BseLI	CCNNNNNNNGG	5 cut(s)	53, 261, 262, 419, 954
BseMII	CTCAG	5 cut(s)	210, 440, 445, 1106, 1203
BseNI	ACTGG	2 cut(s)	61, 1161
BseRI	GAGGAG	2 cut(s)	276, 512
BseX3I	CGGCCG	1 cut(s)	615
BseXI	GCAGC	3 cut(s)	79, 153, 642
Bsh1236I	CGCG	7 cut(s)	292, 509, 511, 528, 627, 839, 1010
Bsh1285I	CGRYCG	2 cut(s)	618, 1079
BshFI	GGCC	8 cut(s)	60, 129, 266, 599, 617, 630, 985, 1178
BshNI	GGYRCC	2 cut(s)	516, 1012
BsiEI	CGRYCG	2 cut(s)	618, 1079
BsiHKAI	GWGCWC	3 cut(s)	648, 926, 938
BsiHKCI	CYCGRG	4 cut(s)	255, 854, 868, 899
BslI	CCNNNNNNNGG	5 cut(s)	53, 261, 262, 419, 954
BsmAI	GTCTC	5 cut(s)	107, 216, 336, 500, 1116
BsmI	GAATGC	1 cut(s)	1162
BsnI	GGCC	8 cut(s)	60, 129, 266, 599, 617, 630, 985, 1178
BsoBI	CYCGRG	4 cut(s)	255, 854, 868, 899
Bsp119I	TTCGAA	1 cut(s)	729
Bsp1286I	GDGCHC	5 cut(s)	414, 648, 849, 926, 938
Bsp143I	GATC	2 cut(s)	74, 579
Bsp1720I	GCTNAGC	2 cut(s)	449, 1115
BspANI	GGCC	8 cut(s)	60, 129, 266, 599, 617, 630, 985, 1178
BspCNI	CTCAG	5 cut(s)	211, 441, 444, 1107, 1204
BspFNI	CGCG	7 cut(s)	292, 509, 511, 528, 627, 839, 1010
BspLI	GGNNCC	7 cut(s)	20, 411, 518, 693, 803, 846, 1014
BspT104I	TTCGAA	1 cut(s)	729
BspT107I	GGYRCC	2 cut(s)	516, 1012
BsrBI	CCGCTC	1 cut(s)	826
BsrFI	RCCGGY	1 cut(s)	658
BsrI	ACTGG	2 cut(s)	61, 1161
BssAI	RCCGGY	1 cut(s)	658
BssECI	CCNNGG	4 cut(s)	267, 612, 868, 1173
BssMI	GATC	2 cut(s)	74, 579
BssNI	GRCGYC	1 cut(s)	971
BssSI	CACGAG	2 cut(s)	587, 1025
Bst2BI	CACGAG	2 cut(s)	587, 1025
Bst2UI	CCWGG	1 cut(s)	268
Bst6I	CTCTTC	2 cut(s)	473, 726
BstACI	GRCGYC	1 cut(s)	971
BstBAI	YACGTR	1 cut(s)	160
BstBI	TTCGAA	1 cut(s)	729
BstC8I	GCNNGC	2 cut(s)	532, 824
BstDEI	CTNAG	6 cut(s)	219, 321, 431, 449, 1115, 1212
BstDSI	CCRYGG	1 cut(s)	1173
BstF5I	GGATG	4 cut(s)	385, 462, 1098, 1116
BstFNI	CGCG	7 cut(s)	292, 509, 511, 528, 627, 839, 1010
BstH2I	RGCGCY	1 cut(s)	456
BstHHI	GCGC	7 cut(s)	72, 455, 511, 530, 627, 710, 839
BstKTI	GATC	2 cut(s)	77, 582
BstMAI	GTCTC	5 cut(s)	107, 216, 336, 500, 1116
BstMBI	GATC	2 cut(s)	74, 579
BstMCI	CGRYCG	2 cut(s)	618, 1079
BstNI	CCWGG	1 cut(s)	268
BstNSI	RCATGY	2 cut(s)	141, 799
BstUI	CGCG	7 cut(s)	292, 509, 511, 528, 627, 839, 1010
BstV1I	GCAGC	3 cut(s)	79, 153, 642
BstV2I	GAAGAC	1 cut(s)	686
BstZI	CGGCCG	1 cut(s)	615
BsuRI	GGCC	8 cut(s)	60, 129, 266, 599, 617, 630, 985, 1178
BtgI	CCRYGG	1 cut(s)	1173
BtgZI	GCGATG	1 cut(s)	526
BtsCI	GGATG	4 cut(s)	385, 462, 1098, 1116
BtsI	GCAGTG	1 cut(s)	151
BtsIMutI	CAGTG	1 cut(s)	151
Cac8I	GCNNGC	2 cut(s)	532, 824
CfoI	GCGC	7 cut(s)	72, 455, 511, 530, 627, 710, 839
Cfr10I	RCCGGY	1 cut(s)	658
Cfr13I	GGNCC	2 cut(s)	264, 983
Cfr9I	CCCGGG	1 cut(s)	868
CseI	GACGC	1 cut(s)	281
Csp6I	GTAC	1 cut(s)	517
CviAII	CATG	5 cut(s)	32, 138, 337, 622, 796
CviQI	GTAC	1 cut(s)	517
DdeI	CTNAG	6 cut(s)	219, 321, 431, 449, 1115, 1212
DpnI	GATC	2 cut(s)	76, 581
DpnII	GATC	2 cut(s)	74, 579
DraI	TTTAAA	1 cut(s)	1105
EaeI	YGGCCR	4 cut(s)	58, 127, 615, 1176
EagI	CGGCCG	1 cut(s)	615
Eam1104I	CTCTTC	2 cut(s)	473, 726
EarI	CTCTTC	2 cut(s)	473, 726
EciI	GGCGGA	2 cut(s)	362, 1064
Ecl136II	GAGCTC	1 cut(s)	924
EclXI	CGGCCG	1 cut(s)	615
Eco24I	GRGCYC	3 cut(s)	414, 849, 926
Eco32I	GATATC	1 cut(s)	1006
Eco52I	CGGCCG	1 cut(s)	615
Eco53kI	GAGCTC	1 cut(s)	924
Eco88I	CYCGRG	4 cut(s)	255, 854, 868, 899
EcoICRI	GAGCTC	1 cut(s)	924
EcoRI	GAATTC	1 cut(s)	194
EcoRII	CCWGG	1 cut(s)	266
EcoRV	GATATC	1 cut(s)	1006
EcoT38I	GRGCYC	3 cut(s)	414, 849, 926
FaeI	CATG	5 cut(s)	35, 141, 340, 625, 799
FaiI	YATR	9 cut(s)	33, 118, 139, 338, 344, 446, 623, 780, 797
FalI	AAGNNNNNCTT	2 cut(s)	758, 790
FatI	CATG	5 cut(s)	31, 137, 336, 621, 795
FauI	CCCGC	2 cut(s)	539, 831
FblI	GTMKAC	2 cut(s)	386, 669
FokI	GGATG	4 cut(s)	392, 449, 1105, 1123
FriOI	GRGCYC	3 cut(s)	414, 849, 926
FspBI	CTAG	4 cut(s)	183, 230, 242, 357
GlaI	GCGC	7 cut(s)	71, 454, 510, 529, 626, 709, 838
GsuI	CTGGAG	1 cut(s)	722
HaeII	RGCGCY	1 cut(s)	456
HaeIII	GGCC	8 cut(s)	60, 129, 266, 599, 617, 630, 985, 1178
HgaI	GACGC	1 cut(s)	281
HhaI	GCGC	7 cut(s)	72, 455, 511, 530, 627, 710, 839
Hin1I	GRCGYC	1 cut(s)	971
Hin1II	CATG	5 cut(s)	35, 141, 340, 625, 799
Hin6I	GCGC	7 cut(s)	70, 453, 509, 528, 625, 708, 837
HinP1I	GCGC	7 cut(s)	70, 453, 509, 528, 625, 708, 837
HincII	GTYRAC	1 cut(s)	296
HindII	GTYRAC	1 cut(s)	296
HphI	GGTGA	1 cut(s)	212
Hpy166II	GTNNAC	3 cut(s)	296, 387, 670
Hpy188I	TCNGA	6 cut(s)	409, 724, 862, 940, 1045, 1092
Hpy188III	TCNNGA	2 cut(s)	701, 757
Hpy8I	GTNNAC	3 cut(s)	296, 387, 670
Hpy99I	CGWCG	6 cut(s)	409, 479, 638, 976, 979, 1063
HpyAV	CCTTC	3 cut(s)	984, 1158, 1199
HpyCH4IV	ACGT	2 cut(s)	159, 971
HpyCH4V	TGCA	3 cut(s)	31, 141, 166
HpyF3I	CTNAG	6 cut(s)	219, 321, 431, 449, 1115, 1212
HpySE526I	ACGT	2 cut(s)	159, 971
Hsp92I	GRCGYC	1 cut(s)	971
Hsp92II	CATG	5 cut(s)	35, 141, 340, 625, 799
HspAI	GCGC	7 cut(s)	70, 453, 509, 528, 625, 708, 837
KpnI	GGTACC	1 cut(s)	520
Kzo9I	GATC	2 cut(s)	74, 579
LmnI	GCTCC	7 cut(s)	24, 409, 691, 844, 929, 941, 1175
Lsp1109I	GCAGC	3 cut(s)	79, 153, 642
LweI	GCATC	2 cut(s)	1083, 1101
MaeI	CTAG	4 cut(s)	183, 230, 242, 357
MaeII	ACGT	2 cut(s)	159, 971
MaeIII	GTNAC	1 cut(s)	905
MalI	GATC	2 cut(s)	76, 581
MbiI	CCGCTC	1 cut(s)	826
MboI	GATC	2 cut(s)	74, 579
MboII	GAAGA	5 cut(s)	460, 463, 574, 691, 743
MhlI	GDGCHC	5 cut(s)	414, 648, 849, 926, 938
MlsI	TGGCCA	1 cut(s)	129
MluCI	AATT	4 cut(s)	174, 194, 246, 276
MluNI	TGGCCA	1 cut(s)	129
MlyI	GAGTC	9 cut(s)	45, 212, 338, 647, 668, 866, 1027, 1061, 1096
MmeI	TCCRAC	6 cut(s)	239, 387, 702, 1023, 1070, 1164
Mox20I	TGGCCA	1 cut(s)	129
MscI	TGGCCA	1 cut(s)	129
MseI	TTAA	1 cut(s)	1104
MslI	CAYNNNNRTG	2 cut(s)	341, 485
Msp20I	TGGCCA	1 cut(s)	129
MspA1I	CMGCKG	1 cut(s)	435
MssI	GTTTAAAC	1 cut(s)	1105
Mva1269I	GAATGC	1 cut(s)	1162
MvaI	CCWGG	1 cut(s)	268
MvnI	CGCG	7 cut(s)	292, 509, 511, 528, 627, 839, 1010
NciI	CCSGG	9 cut(s)	377, 614, 689, 869, 870, 915, 928, 949, 1153
NdeII	GATC	2 cut(s)	74, 579
NlaIII	CATG	5 cut(s)	35, 141, 340, 625, 799
NlaIV	GGNNCC	7 cut(s)	20, 411, 518, 693, 803, 846, 1014
NmeAIII	GCCGAG	3 cut(s)	1011, 1041, 1204
NspI	RCATGY	2 cut(s)	141, 799
NspV	TTCGAA	1 cut(s)	729
PciI	ACATGT	1 cut(s)	795
PcsI	WCGNNNNNNNCGW	4 cut(s)	735, 789, 983, 1067
PctI	GAATGC	1 cut(s)	1162
PfeI	GAWTC	1 cut(s)	697
PflFI	GACNNNGTC	2 cut(s)	343, 973
PfoI	TCCNGGA	1 cut(s)	1151
PleI	GAGTC	9 cut(s)	44, 211, 337, 646, 668, 865, 1026, 1060, 1095
PmeI	GTTTAAAC	1 cut(s)	1105
PpsI	GAGTC	9 cut(s)	44, 211, 337, 646, 668, 865, 1026, 1060, 1095
Ppu21I	YACGTR	1 cut(s)	160
PscI	ACATGT	1 cut(s)	795
Psp124BI	GAGCTC	1 cut(s)	926
Psp6I	CCWGG	1 cut(s)	266
PspGI	CCWGG	1 cut(s)	266
PspN4I	GGNNCC	7 cut(s)	20, 411, 518, 693, 803, 846, 1014
PspPI	GGNCC	2 cut(s)	264, 983
PsyI	GACNNNGTC	2 cut(s)	343, 973
RsaI	GTAC	1 cut(s)	518
RsaNI	GTAC	1 cut(s)	517
RseI	CAYNNNNRTG	2 cut(s)	341, 485
SacI	GAGCTC	1 cut(s)	926
SaqAI	TTAA	1 cut(s)	1104
Sau3AI	GATC	2 cut(s)	74, 579
Sau96I	GGNCC	2 cut(s)	264, 983
SchI	GAGTC	9 cut(s)	45, 212, 338, 647, 668, 866, 1027, 1061, 1096
SduI	GDGCHC	5 cut(s)	414, 648, 849, 926, 938
SfaNI	GCATC	2 cut(s)	1083, 1101
SfuI	TTCGAA	1 cut(s)	729
SmaI	CCCGGG	1 cut(s)	870
SmiMI	CAYNNNNRTG	2 cut(s)	341, 485
SmlI	CTYRAG	1 cut(s)	647
SmoI	CTYRAG	1 cut(s)	647
SpeI	ACTAGT	1 cut(s)	241
Sse9I	AATT	4 cut(s)	174, 194, 246, 276
SspMI	CTAG	4 cut(s)	183, 230, 242, 357
SstI	GAGCTC	1 cut(s)	926
TaiI	ACGT	2 cut(s)	162, 974
TaqII	GACCGA	1 cut(s)	1065
TasI	AATT	4 cut(s)	174, 194, 246, 276
TfiI	GAWTC	1 cut(s)	697
Tru1I	TTAA	1 cut(s)	1104
Tru9I	TTAA	1 cut(s)	1104
TscAI	CASTG	1 cut(s)	151
TseI	GCWGC	3 cut(s)	92, 141, 655
TspGWI	ACGGA	3 cut(s)	16, 1123, 1162
TspMI	CCCGGG	1 cut(s)	868
TspRI	CASTG	1 cut(s)	151
Tth111I	GACNNNGTC	2 cut(s)	343, 973
XapI	RAATTY	2 cut(s)	174, 194
XceI	RCATGY	2 cut(s)	141, 799
XmaI	CCCGGG	1 cut(s)	868
XmiI	GTMKAC	2 cut(s)	386, 669
XspI	CTAG	4 cut(s)	183, 230, 242, 357
ZraI	GACGTC	1 cut(s)	972

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.