pycom13g20250

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005053
Physical Location & Seq
Reverse (-)
16280413 .. 16281171
759 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
ATGTTCAAGCAGACATTTTCGTCTATTTTCACCGTACTGCTGATTGCGAACATGCAGACAGCAATACCACTTGTGGCTGCAGTTGAACCTGCAGCCGTTGGCAGCGAACCAGTTCTGGAAATATACATGCATGACATTCTTGGAGGGAGCATCCCTACTGCTAGGCCAATCACTGGCTTGCTAGGCAATATATACAGCAGTGAAGTGCCCTTTGCAAAGCCGATAGGGTTCCTTCCTCCAGATGGTGCTGTGGCTATTCCTAATGCCAACGGTGCTATTCCTACTGTGAATGGCGCCAATGGTCTCCCACTAGGAACCGGCTTATCTGGTACATCCTTTGCCGGAAATCCTAATGGTGAAAAGAATGGCAATACAGCTACCCAACTGGCACCAGACGGGTTGGGACTAGGCTTTGGGACAGTCACCGTTATTGATGACATACTGACCTCTAGTCCCGAATTGGGCTCACAGACAATTGGGAAAGCTCAAGGAGTCTATGTGGCAAGCTCTGCCGATGGATCAAGTCAGCTGATGGCATTTACGGCTATGATGGAAGGAGGGGAGTACAATGATAACCTCAACTTCTTCGGAGTGTACAAGATTGGGAGTAGCAAGTCGCAACTGTCAGTGACTGGAGGGACGGGCAAATTCAAGAATGCCAATGGAATTGCAGAGTTGAAACCACTGATTCCTCCAGGTCAACGAGCAACAGATGGTGCAGAAACATTGCTGAGGATTACCATTCACTTGAAGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

253

Amino Acids

25.77

Weight (kDa)

4.97

Isoelectric Point (pI)

24.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 134 - 228 4e-25 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 97
AccB1I GGYRCC 2 cut(s) 293, 388
AccB7I CCANNNNNTGG 1 cut(s) 173
AclWI GGATC 1 cut(s) 526
AcsI RAATTY 1 cut(s) 647
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 5 cut(s) 36, 331, 566, 596, 755
AfiI CCNNNNNNNGG 3 cut(s) 173, 242, 461
AgsI TTSAA 5 cut(s) 7, 86, 652, 679, 751
AhdI GACNNNNNGTC 1 cut(s) 450
AjnI CCWGG 1 cut(s) 694
AluBI AGCT 4 cut(s) 377, 485, 507, 529
AluI AGCT 4 cut(s) 377, 485, 507, 529
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 1 cut(s) 526
AlwNI CAGNNNCTG 1 cut(s) 632
AoxI GGCC 1 cut(s) 164
ApeKI GCWGC 3 cut(s) 77, 92, 102
ApoI RAATTY 1 cut(s) 647
Asp700I GAANNNNTTC 1 cut(s) 111
AspLEI GCGC 1 cut(s) 296
AsuHPI GGTGA 3 cut(s) 22, 368, 415
BaeGI GKGCMC 1 cut(s) 210
BanI GGYRCC 2 cut(s) 293, 388
BanII GRGCYC 1 cut(s) 467
BbvCI CCTCAGC 1 cut(s) 731
BbvI GCAGC 3 cut(s) 64, 104, 114
BccI CCATC 5 cut(s) 236, 509, 526, 544, 707
BceAI ACGGC 2 cut(s) 80, 558
BciT130I CCWGG 1 cut(s) 696
BcoDI GTCTC 1 cut(s) 308
BfaI CTAG 5 cut(s) 162, 182, 311, 407, 450
BfmI CTRYAG 2 cut(s) 78, 90
BfoI RGCGCY 1 cut(s) 297
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 3 cut(s) 78, 93, 103
BlsI GCNGC 3 cut(s) 79, 94, 104
BmcAI AGTACT 1 cut(s) 755
Bme1390I CCNGG 1 cut(s) 696
BmeRI GACNNNNNGTC 1 cut(s) 450
BmiI GGNNCC 4 cut(s) 230, 295, 316, 390
BmrFI CCNGG 1 cut(s) 696
BmsI GCATC 1 cut(s) 159
BpmI CTGGAG 3 cut(s) 222, 654, 678
Bpu10I CCTNAGC 1 cut(s) 731
BpuEI CTTGAG 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 294
BsaI GGTCTC 1 cut(s) 308
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 242, 461
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 4 cut(s) 110, 178, 390, 637
Bse3DI GCAATG 1 cut(s) 725
BseBI CCWGG 1 cut(s) 696
BseGI GGATG 2 cut(s) 150, 332
BseLI CCNNNNNNNGG 3 cut(s) 173, 242, 461
BseMI GCAATG 1 cut(s) 725
BseMII CTCAG 1 cut(s) 722
BseNI ACTGG 4 cut(s) 110, 178, 390, 637
BseSI GKGCMC 1 cut(s) 210
BseXI GCAGC 3 cut(s) 64, 104, 114
BsgI GTGCAG 1 cut(s) 738
BshFI GGCC 1 cut(s) 166
BshNI GGYRCC 2 cut(s) 293, 388
BsiSI CCGG 2 cut(s) 318, 342
BslFI GGGAC 4 cut(s) 417, 430, 438, 652
BslI CCNNNNNNNGG 3 cut(s) 173, 242, 461
BsmAI GTCTC 1 cut(s) 308
BsmFI GGGAC 4 cut(s) 417, 430, 438, 652
BsmI GAATGC 1 cut(s) 661
BsnI GGCC 1 cut(s) 166
Bso31I GGTCTC 1 cut(s) 308
Bsp1286I GDGCHC 2 cut(s) 210, 467
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 1 cut(s) 518
BspANI GGCC 1 cut(s) 166
BspCNI CTCAG 1 cut(s) 723
BspLI GGNNCC 4 cut(s) 230, 295, 316, 390
BspMAI CTGCAG 2 cut(s) 82, 94
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 1 cut(s) 526
BspT107I GGYRCC 2 cut(s) 293, 388
BspTNI GGTCTC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 725
BsrFI RCCGGY 1 cut(s) 317
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 4 cut(s) 110, 178, 390, 637
BssAI RCCGGY 1 cut(s) 317
BssMI GATC 1 cut(s) 518
BssNI GRCGYC 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 696
Bst4CI ACNGT 6 cut(s) 34, 272, 286, 421, 427, 624
BstACI GRCGYC 1 cut(s) 294
BstAPI GCANNNNNTGC 1 cut(s) 509
BstAUI TGTACA 1 cut(s) 594
BstC8I GCNNGC 2 cut(s) 179, 505
BstDEI CTNAG 1 cut(s) 731
BstF5I GGATG 2 cut(s) 150, 332
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 1 cut(s) 521
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 1 cut(s) 518
BstMWI GCNNNNNNNGC 4 cut(s) 183, 272, 509, 542
BstNI CCWGG 1 cut(s) 696
BstNSI RCATGY 2 cut(s) 55, 130
BstSCI CCNGG 1 cut(s) 694
BstSFI CTRYAG 2 cut(s) 78, 90
BstSLI GKGCMC 1 cut(s) 210
BstV1I GCAGC 3 cut(s) 64, 104, 114
BsuRI GGCC 1 cut(s) 166
BtsCI GGATG 2 cut(s) 150, 332
BtsI GCAGTG 1 cut(s) 205
BtsIMutI CAGTG 4 cut(s) 171, 205, 633, 683
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 2 cut(s) 179, 505
CaiI CAGNNNCTG 1 cut(s) 632
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 317
Csp6I GTAC 5 cut(s) 35, 330, 565, 595, 754
CviAII CATG 3 cut(s) 52, 127, 131
CviQI GTAC 5 cut(s) 35, 330, 565, 595, 754
DdeI CTNAG 1 cut(s) 731
DinI GGCGCC 1 cut(s) 295
DpnI GATC 1 cut(s) 520
DpnII GATC 1 cut(s) 518
DrdI GACNNNNNNGTC 1 cut(s) 19
DriI GACNNNNNGTC 1 cut(s) 450
DseDI GACNNNNNNGTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 467
Eco31I GGTCTC 1 cut(s) 308
EcoRII CCWGG 1 cut(s) 694
EcoT22I ATGCAT 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 467
EgeI GGCGCC 1 cut(s) 295
EheI GGCGCC 1 cut(s) 295
FaeI CATG 3 cut(s) 55, 130, 134
FaiI YATR 9 cut(s) 53, 124, 128, 132, 191, 193, 440, 498, 548
FaqI GGGAC 4 cut(s) 417, 430, 438, 652
FatI CATG 3 cut(s) 51, 126, 130
Fnu4HI GCNGC 3 cut(s) 78, 93, 103
FokI GGATG 2 cut(s) 137, 319
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 3 cut(s) 78, 93, 103
FspBI CTAG 5 cut(s) 162, 182, 311, 407, 450
GlaI GCGC 1 cut(s) 295
GluI GCNGC 3 cut(s) 78, 93, 103
GsuI CTGGAG 3 cut(s) 222, 654, 678
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 1 cut(s) 166
HapII CCGG 2 cut(s) 318, 342
HhaI GCGC 1 cut(s) 296
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 3 cut(s) 55, 130, 134
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HincII GTYRAC 1 cut(s) 701
HindII GTYRAC 1 cut(s) 701
HinfI GANTC 2 cut(s) 492, 688
HpaII CCGG 2 cut(s) 318, 342
HphI GGTGA 3 cut(s) 22, 368, 415
Hpy166II GTNNAC 2 cut(s) 595, 701
Hpy188I TCNGA 1 cut(s) 590
Hpy188III TCNNGA 4 cut(s) 116, 239, 455, 652
Hpy8I GTNNAC 2 cut(s) 595, 701
HpyAV CCTTC 2 cut(s) 242, 548
HpyCH4III ACNGT 6 cut(s) 34, 272, 286, 421, 427, 624
HpyCH4V TGCA 7 cut(s) 55, 80, 92, 130, 215, 671, 719
HpyF10VI GCNNNNNNNGC 4 cut(s) 183, 272, 509, 542
HpyF3I CTNAG 1 cut(s) 731
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 3 cut(s) 55, 130, 134
HspAI GCGC 1 cut(s) 294
KasI GGCGCC 1 cut(s) 293
Kzo9I GATC 1 cut(s) 518
LmnI GCTCC 1 cut(s) 147
Lsp1109I GCAGC 3 cut(s) 64, 104, 114
LweI GCATC 1 cut(s) 159
MaeI CTAG 5 cut(s) 162, 182, 311, 407, 450
MaeIII GTNAC 2 cut(s) 421, 628
MalI GATC 1 cut(s) 520
MboI GATC 1 cut(s) 518
MboII GAAGA 1 cut(s) 577
MfeI CAATTG 1 cut(s) 474
MhlI GDGCHC 2 cut(s) 210, 467
MluCI AATT 4 cut(s) 458, 474, 647, 666
Mly113I GGCGCC 1 cut(s) 294
MlyI GAGTC 1 cut(s) 501
MnlI CCTC 8 cut(s) 137, 246, 457, 551, 587, 629, 702, 726
Mph1103I ATGCAT 1 cut(s) 132
MroXI GAANNNNTTC 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 529
MspI CCGG 2 cut(s) 318, 342
MspR9I CCNGG 1 cut(s) 696
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 1 cut(s) 661
MvaI CCWGG 1 cut(s) 696
MwoI GCNNNNNNNGC 4 cut(s) 183, 272, 509, 542
NarI GGCGCC 1 cut(s) 294
NdeII GATC 1 cut(s) 518
NlaIII CATG 3 cut(s) 55, 130, 134
NlaIV GGNNCC 4 cut(s) 230, 295, 316, 390
NmuCI GTSAC 2 cut(s) 421, 628
NsiI ATGCAT 1 cut(s) 132
NspI RCATGY 2 cut(s) 55, 130
PctI GAATGC 1 cut(s) 661
PdmI GAANNNNTTC 1 cut(s) 111
PfeI GAWTC 1 cut(s) 688
PflMI CCANNNNNTGG 1 cut(s) 173
PkrI GCNGC 3 cut(s) 79, 94, 104
PleI GAGTC 1 cut(s) 500
PluTI GGCGCC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 500
Psp6I CCWGG 1 cut(s) 694
PspGI CCWGG 1 cut(s) 694
PspN4I GGNNCC 4 cut(s) 230, 295, 316, 390
PstI CTGCAG 2 cut(s) 82, 94
PstNI CAGNNNCTG 1 cut(s) 632
PvuII CAGCTG 1 cut(s) 529
RsaI GTAC 5 cut(s) 36, 331, 566, 596, 755
RsaNI GTAC 5 cut(s) 35, 330, 565, 595, 754
SatI GCNGC 3 cut(s) 78, 93, 103
Sau3AI GATC 1 cut(s) 518
ScaI AGTACT 1 cut(s) 755
SchI GAGTC 1 cut(s) 501
ScrFI CCNGG 1 cut(s) 696
SduI GDGCHC 2 cut(s) 210, 467
SetI ASST 8 cut(s) 91, 379, 449, 487, 509, 531, 579, 700
SfaNI GCATC 1 cut(s) 159
SfcI CTRYAG 2 cut(s) 78, 90
SfoI GGCGCC 1 cut(s) 295
SmlI CTYRAG 1 cut(s) 486
SmoI CTYRAG 1 cut(s) 486
Sse9I AATT 4 cut(s) 458, 474, 647, 666
SspDI GGCGCC 1 cut(s) 293
SspMI CTAG 5 cut(s) 162, 182, 311, 407, 450
StyD4I CCNGG 1 cut(s) 694
TaaI ACNGT 6 cut(s) 34, 272, 286, 421, 427, 624
TasI AATT 4 cut(s) 458, 474, 647, 666
TatI WGTACW 3 cut(s) 564, 594, 753
TfiI GAWTC 1 cut(s) 688
TscAI CASTG 4 cut(s) 178, 205, 633, 690
TseFI GTSAC 2 cut(s) 421, 628
TseI GCWGC 3 cut(s) 77, 92, 102
Tsp45I GTSAC 2 cut(s) 421, 628
TspRI CASTG 4 cut(s) 178, 205, 633, 690
Van91I CCANNNNNTGG 1 cut(s) 173
XapI RAATTY 1 cut(s) 647
XceI RCATGY 2 cut(s) 55, 130
XmnI GAANNNNTTC 1 cut(s) 111
XspI CTAG 5 cut(s) 162, 182, 311, 407, 450
ZrmI AGTACT 1 cut(s) 755
Zsp2I ATGCAT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.