pycom13g22680

Yip1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006639
Physical Location & Seq
Forward (+)
20416027 .. 20417568
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 837 bp
ATGTCTCACTCCGAACGCGACACCGTCCCCCTCCACCCCTCCTCTCAATCCGACATCGACGAGATCGAAAACCTAATCAACGCCAGCGTCCAATCGGGCCCCACCAACGTCCTCCCGGCCAAACCCCCCAGTCCTCCCCGCGCCTCCATTCCCGTCTCATCATCCCCTTTCATCCCATCAAACCTCCCACCCCCAGCCCCCTCCTCCGCCGCAAAGAAGCCTTCCTCCGTCCCCGCCCCACCTCCCATTCCCTCCGCAACCAACGGCCCCAGTATCTTCCCCTCCGGGTTCGGCTCCCCGCCCAACACCCTAACCGAGCCCGTTTGGGACACCGTGAAGCGCGACTTGTCGAGAATCGTGAGCAATTTGAAGCTTGTGGTGTTCCCCAACCCCTATCGTGAGGATCCGGGAAAGGCCTTGAGGGATTGGGATCTCTGGGGACCTTTCTTCTTCATTGTATTCTTGGGTCTCACACTTTCCTGGTCTGCATCTGTTAAAAAGTCAGAGGTTTTTGCTGTTGCTTTTGCTGTGCTTGCTGCTGGTGCTGTGATTCTGACACTGAATGTACTCCTACTGGGTGGACACATAATATTCTTCCAGAGCCTTAGTCTTCTCGGTTATTGCTTATTTCCTCTGGACGTTGGAGCTCTGATATGTATGGTGAAGGATTATGTGATATTGAAGGTGGTGGTGGTAAGCGTAACACTGGCTTGGAGTTCATGGGCTGCATATCCTTTCATGAGTTCAGCGGTCAACCCCAGGAGAAAAGCCCTTGCACTTTACCCTGTTTTCCTATTGTATGTATCCATCGGTTTCCTTATTATCGCCATTGATTGA

Protein Analysis

279

Amino Acids

29.85

Weight (kDa)

8.52

Isoelectric Point (pI)

58.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yip1 PF04893 140 - 272 1.3e-15 Yip1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 18, 141, 342
AciI CCGC 7 cut(s) 139, 207, 210, 234, 255, 299, 749
AclWI GGATC 3 cut(s) 398, 411, 438
AcoI YGGCCR 1 cut(s) 117
AfaI GTAC 1 cut(s) 567
AgsI TTSAA 2 cut(s) 370, 682
AjnI CCWGG 2 cut(s) 479, 758
AluBI AGCT 2 cut(s) 373, 647
AluI AGCT 2 cut(s) 373, 647
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 3 cut(s) 9, 160, 473
AlwI GGATC 3 cut(s) 398, 411, 438
AoxI GGCC 4 cut(s) 97, 117, 265, 414
ApaI GGGCCC 1 cut(s) 101
ApeKI GCWGC 2 cut(s) 536, 725
AspLEI GCGC 2 cut(s) 143, 342
AspS9I GGNCC 4 cut(s) 97, 98, 266, 440
AsuC2I CCSGG 3 cut(s) 116, 286, 408
AsuHPI GGTGA 1 cut(s) 673
AvaII GGWCC 1 cut(s) 440
BaeGI GKGCMC 1 cut(s) 101
BamHI GGATCC 1 cut(s) 403
BanII GRGCYC 3 cut(s) 101, 321, 649
BbsI GAAGAC 1 cut(s) 602
Bbv12I GWGCWC 1 cut(s) 649
BbvI GCAGC 2 cut(s) 523, 712
BccI CCATC 2 cut(s) 184, 815
BceAI ACGGC 1 cut(s) 280
BciT130I CCWGG 2 cut(s) 481, 760
BciVI GTATCC 1 cut(s) 814
BcnI CCSGG 3 cut(s) 116, 286, 408
BcoDI GTCTC 3 cut(s) 9, 160, 473
BfuI GTATCC 1 cut(s) 814
BisI GCNGC 3 cut(s) 210, 537, 726
BlsI GCNGC 3 cut(s) 211, 538, 727
Bme1390I CCNGG 5 cut(s) 116, 286, 408, 481, 760
Bme18I GGWCC 1 cut(s) 440
BmgT120I GGNCC 4 cut(s) 97, 98, 266, 440
BmiI GGNNCC 6 cut(s) 99, 100, 268, 295, 405, 441
BmrFI CCNGG 5 cut(s) 116, 286, 408, 481, 760
BmrI ACTGGG 3 cut(s) 123, 264, 584
BmsI GCATC 1 cut(s) 497
BmuI ACTGGG 3 cut(s) 123, 264, 584
BpiI GAAGAC 1 cut(s) 602
BpuEI CTTGAG 1 cut(s) 439
BpuMI CCSGG 3 cut(s) 116, 286, 408
BsaBI GATNNNNATC 1 cut(s) 429
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 1 cut(s) 758
Bse1I ACTGG 4 cut(s) 129, 270, 579, 711
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 2 cut(s) 481, 760
BseDI CCNNGG 1 cut(s) 758
BseGI GGATG 2 cut(s) 161, 171
BseJI GATNNNNATC 1 cut(s) 429
BseNI ACTGG 4 cut(s) 129, 270, 579, 711
BseRI GAGGAG 2 cut(s) 31, 193
BseSI GKGCMC 1 cut(s) 101
BseXI GCAGC 2 cut(s) 523, 712
BseYI CCCAGC 1 cut(s) 193
Bsh1236I CGCG 3 cut(s) 18, 141, 342
BshFI GGCC 4 cut(s) 99, 119, 267, 416
BsiHKAI GWGCWC 1 cut(s) 649
BsiSI CCGG 3 cut(s) 116, 285, 407
BslFI GGGAC 4 cut(s) 11, 215, 341, 453
BsmAI GTCTC 3 cut(s) 9, 160, 473
BsmBI CGTCTC 1 cut(s) 160
BsmFI GGGAC 4 cut(s) 11, 215, 341, 453
BsnI GGCC 4 cut(s) 99, 119, 267, 416
Bso31I GGTCTC 1 cut(s) 473
Bsp120I GGGCCC 1 cut(s) 97
Bsp1286I GDGCHC 3 cut(s) 101, 321, 649
Bsp143I GATC 3 cut(s) 63, 403, 430
BspACI CCGC 7 cut(s) 139, 207, 210, 234, 255, 299, 749
BspANI GGCC 4 cut(s) 99, 119, 267, 416
BspFNI CGCG 3 cut(s) 18, 141, 342
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 6 cut(s) 99, 100, 268, 295, 405, 441
BspPI GGATC 3 cut(s) 398, 411, 438
BspTNI GGTCTC 1 cut(s) 473
BsrI ACTGG 4 cut(s) 129, 270, 579, 711
BssECI CCNNGG 1 cut(s) 758
BssMI GATC 3 cut(s) 63, 403, 430
Bst2UI CCWGG 2 cut(s) 481, 760
Bst4CI ACNGT 2 cut(s) 25, 334
BstC8I GCNNGC 2 cut(s) 85, 534
BstDEI CTNAG 1 cut(s) 605
BstF5I GGATG 2 cut(s) 161, 171
BstFNI CGCG 3 cut(s) 18, 141, 342
BstHHI GCGC 2 cut(s) 143, 342
BstKTI GATC 3 cut(s) 66, 406, 433
BstMAI GTCTC 3 cut(s) 9, 160, 473
BstMBI GATC 3 cut(s) 63, 403, 430
BstMWI GCNNNNNNNGC 2 cut(s) 533, 542
BstNI CCWGG 2 cut(s) 481, 760
BstSCI CCNGG 5 cut(s) 114, 284, 406, 479, 758
BstSLI GKGCMC 1 cut(s) 101
BstUI CGCG 3 cut(s) 18, 141, 342
BstV1I GCAGC 2 cut(s) 523, 712
BstV2I GAAGAC 1 cut(s) 602
BstX2I RGATCY 2 cut(s) 403, 430
BstYI RGATCY 2 cut(s) 403, 430
BsuI GTATCC 1 cut(s) 814
BsuRI GGCC 4 cut(s) 99, 119, 267, 416
BtsCI GGATG 2 cut(s) 161, 171
BtsIMutI CAGTG 2 cut(s) 557, 704
Cac8I GCNNGC 2 cut(s) 85, 534
CciI TCATGA 1 cut(s) 738
CfoI GCGC 2 cut(s) 143, 342
Cfr13I GGNCC 4 cut(s) 97, 98, 266, 440
CseI GACGC 1 cut(s) 76
Csp6I GTAC 1 cut(s) 566
CviAII CATG 2 cut(s) 720, 739
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 1 cut(s) 605
DpnI GATC 3 cut(s) 65, 405, 432
DpnII GATC 3 cut(s) 63, 403, 430
EaeI YGGCCR 1 cut(s) 117
EciI GGCGGA 1 cut(s) 196
Ecl136II GAGCTC 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 416
Eco24I GRGCYC 3 cut(s) 101, 321, 649
Eco31I GGTCTC 1 cut(s) 473
Eco47I GGWCC 1 cut(s) 440
Eco53kI GAGCTC 1 cut(s) 647
EcoICRI GAGCTC 1 cut(s) 647
EcoO109I RGGNCCY 2 cut(s) 98, 440
EcoRII CCWGG 2 cut(s) 479, 758
EcoT38I GRGCYC 3 cut(s) 101, 321, 649
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 2 cut(s) 723, 742
FaiI YATR 8 cut(s) 587, 655, 659, 672, 721, 730, 740, 801
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 4 cut(s) 11, 215, 341, 453
FatI CATG 2 cut(s) 719, 738
FauI CCCGC 3 cut(s) 146, 241, 306
Fnu4HI GCNGC 3 cut(s) 210, 537, 726
FokI GGATG 2 cut(s) 148, 158
FriOI GRGCYC 3 cut(s) 101, 321, 649
Fsp4HI GCNGC 3 cut(s) 210, 537, 726
GlaI GCGC 2 cut(s) 142, 341
GluI GCNGC 3 cut(s) 210, 537, 726
GsaI CCCAGC 1 cut(s) 197
HaeIII GGCC 4 cut(s) 99, 119, 267, 416
HapII CCGG 3 cut(s) 116, 285, 407
HgaI GACGC 1 cut(s) 76
HhaI GCGC 2 cut(s) 143, 342
Hin1II CATG 2 cut(s) 723, 742
Hin6I GCGC 2 cut(s) 141, 340
HinP1I GCGC 2 cut(s) 141, 340
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 2 cut(s) 354, 550
HpaII CCGG 3 cut(s) 116, 285, 407
HphI GGTGA 1 cut(s) 673
Hpy166II GTNNAC 2 cut(s) 581, 754
Hpy188I TCNGA 5 cut(s) 13, 52, 505, 555, 651
Hpy188III TCNNGA 6 cut(s) 351, 358, 398, 598, 635, 739
Hpy8I GTNNAC 2 cut(s) 581, 754
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 3 cut(s) 231, 658, 676
HpyCH4III ACNGT 2 cut(s) 25, 334
HpyCH4IV ACGT 2 cut(s) 108, 639
HpyCH4V TGCA 3 cut(s) 488, 728, 776
HpyF10VI GCNNNNNNNGC 2 cut(s) 533, 542
HpyF3I CTNAG 1 cut(s) 605
HpySE526I ACGT 2 cut(s) 108, 639
Hsp92II CATG 2 cut(s) 723, 742
HspAI GCGC 2 cut(s) 141, 340
Kzo9I GATC 3 cut(s) 63, 403, 430
LmnI GCTCC 2 cut(s) 299, 644
Lsp1109I GCAGC 2 cut(s) 523, 712
LweI GCATC 1 cut(s) 497
MaeII ACGT 2 cut(s) 108, 639
MaeIII GTNAC 1 cut(s) 700
MalI GATC 3 cut(s) 65, 405, 432
MboI GATC 3 cut(s) 63, 403, 430
MboII GAAGA 5 cut(s) 268, 439, 442, 586, 602
MflI RGATCY 2 cut(s) 403, 430
MhlI GDGCHC 3 cut(s) 101, 321, 649
MluCI AATT 1 cut(s) 364
MmeI TCCRAC 2 cut(s) 75, 622
MseI TTAA 1 cut(s) 495
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 3 cut(s) 116, 285, 407
MspR9I CCNGG 5 cut(s) 116, 286, 408, 481, 760
MvaI CCWGG 2 cut(s) 481, 760
MvnI CGCG 3 cut(s) 18, 141, 342
MwoI GCNNNNNNNGC 2 cut(s) 533, 542
NciI CCSGG 3 cut(s) 116, 286, 408
NdeII GATC 3 cut(s) 63, 403, 430
NlaIII CATG 2 cut(s) 723, 742
NlaIV GGNNCC 6 cut(s) 99, 100, 268, 295, 405, 441
PagI TCATGA 1 cut(s) 738
PceI AGGCCT 1 cut(s) 416
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PfeI GAWTC 2 cut(s) 354, 550
PflFI GACNNNGTC 1 cut(s) 23
PfoI TCCNGGA 1 cut(s) 406
PkrI GCNGC 3 cut(s) 211, 538, 727
PpuMI RGGWCCY 1 cut(s) 440
Psp124BI GAGCTC 1 cut(s) 649
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 2 cut(s) 479, 758
PspFI CCCAGC 1 cut(s) 193
PspGI CCWGG 2 cut(s) 479, 758
PspN4I GGNNCC 6 cut(s) 99, 100, 268, 295, 405, 441
PspOMI GGGCCC 1 cut(s) 97
PspPI GGNCC 4 cut(s) 97, 98, 266, 440
PspPPI RGGWCCY 1 cut(s) 440
PsuI RGATCY 2 cut(s) 403, 430
PsyI GACNNNGTC 1 cut(s) 23
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
SacI GAGCTC 1 cut(s) 649
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 3 cut(s) 210, 537, 726
Sau3AI GATC 3 cut(s) 63, 403, 430
Sau96I GGNCC 4 cut(s) 97, 98, 266, 440
ScrFI CCNGG 5 cut(s) 116, 286, 408, 481, 760
SduI GDGCHC 3 cut(s) 101, 321, 649
SfaNI GCATC 1 cut(s) 497
SinI GGWCC 1 cut(s) 440
SmlI CTYRAG 1 cut(s) 418
SmoI CTYRAG 1 cut(s) 418
Sse9I AATT 1 cut(s) 364
SseBI AGGCCT 1 cut(s) 416
SsiI CCGC 7 cut(s) 139, 207, 210, 234, 255, 299, 749
SspI AATATT 1 cut(s) 591
SstI GAGCTC 1 cut(s) 649
StuI AGGCCT 1 cut(s) 416
StyD4I CCNGG 5 cut(s) 114, 284, 406, 479, 758
TaaI ACNGT 2 cut(s) 25, 334
TaiI ACGT 2 cut(s) 111, 642
TaqI TCGA 3 cut(s) 57, 66, 350
TasI AATT 1 cut(s) 364
TatI WGTACW 1 cut(s) 565
TauI GCSGC 1 cut(s) 212
TfiI GAWTC 2 cut(s) 354, 550
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TscAI CASTG 2 cut(s) 564, 711
TseI GCWGC 2 cut(s) 536, 725
TspDTI ATGAA 4 cut(s) 160, 442, 708, 727
TspGWI ACGGA 1 cut(s) 217
TspRI CASTG 2 cut(s) 564, 711
Tth111I GACNNNGTC 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.