pycom14g04630

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009153
Physical Location & Seq
Reverse (-)
4311666 .. 4316429
4764 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 756 bp
ATGAACACAGCCATGAAAGCGGCTCTCACAAACTCCAAACTCAACTGCTGCATTTATGTGAGGGCCGCCCTCTTTCATTCCACCCCCGTCTTGGATCGTAAGCGACGCTCCTACTGGCAACCTAGATTCAACAACCATGGAAGAAGATCCAGAAGGCTCCATGGAAAGCAAGCGTTACTGAGGAATGTCAATGCTTATGCAGACTTCTTATTTCAGAGTTGGCAGAACGAATACGATGAGGAGGAGGAGCCATCATCAAGTAGAGGCACCTCATGGTTTAAAAAACAATACTCAGCCAAGGGGTCCAAAAAGAAGTCAGGCGATCAAGGAGCCTGGAATTACGGAAGAAATGATTTCTGGTTTGAAGAAGATGGAGATGTGGAGACGATTTTCCGATCCATGTTTGGTGGAAACAAATTTTATTATTGGTCATTCATTAATGAAGAGAATTCACAGCGGAGGAGCTCCAGTAATTACTCTAACTATGGAAGGTCTTGGAATTGGAGACATAACAATAAGGAAGAGTATCAATATGACTCTGAGCCTGAGAATTTGGAGTCAGATGAAAGGTTGGCCCTCGGGTTGCGTGCTTCGGGCCCTCTAACACTTGATGATGTCAAGAATGCATATCGAATCTGTGCGCTGAAATGGCATCCAGACCGTCACCAAGGTTCTTCCAAGGTGGCTGCAGAGGAAAAGTTCAAGCTTTGCAGCGCGGCATATCAATCATTATGCGGTAAATTGGCTGTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

252

Amino Acids

29.38

Weight (kDa)

9.25

Isoelectric Point (pI)

58.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 201 - 245 4.7e-10 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 266
AccII CGCG 1 cut(s) 716
AciI CCGC 5 cut(s) 20, 66, 457, 716, 735
AclWI GGATC 3 cut(s) 102, 141, 390
AcsI RAATTY 3 cut(s) 416, 448, 550
AfiI CCNNNNNNNGG 1 cut(s) 91
AgsI TTSAA 3 cut(s) 130, 365, 703
AjnI CCWGG 1 cut(s) 332
AluBI AGCT 2 cut(s) 465, 706
AluI AGCT 2 cut(s) 465, 706
Alw21I GWGCWC 1 cut(s) 467
Alw26I GTCTC 2 cut(s) 377, 499
AlwI GGATC 3 cut(s) 102, 141, 390
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 3 cut(s) 63, 573, 595
ApaI GGGCCC 1 cut(s) 599
ApeKI GCWGC 3 cut(s) 48, 686, 711
ApoI RAATTY 3 cut(s) 416, 448, 550
AseI ATTAAT 1 cut(s) 438
AspLEI GCGC 2 cut(s) 643, 716
AspS9I GGNCC 5 cut(s) 63, 303, 574, 595, 596
AsuHPI GGTGA 1 cut(s) 656
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 599
BanI GGYRCC 1 cut(s) 266
BanII GRGCYC 2 cut(s) 467, 599
Bbv12I GWGCWC 1 cut(s) 467
BbvI GCAGC 3 cut(s) 35, 673, 723
BccI CCATC 2 cut(s) 259, 365
BciT130I CCWGG 1 cut(s) 334
BcoDI GTCTC 2 cut(s) 377, 499
BfaI CTAG 1 cut(s) 123
BfmI CTRYAG 1 cut(s) 687
BisI GCNGC 6 cut(s) 21, 49, 66, 687, 712, 717
BlsI GCNGC 6 cut(s) 22, 50, 67, 688, 713, 718
Bme1390I CCNGG 1 cut(s) 334
Bme18I GGWCC 1 cut(s) 303
BmeT110I CYCGRG 1 cut(s) 578
BmgT120I GGNCC 5 cut(s) 63, 303, 574, 595, 596
BmiI GGNNCC 6 cut(s) 158, 249, 268, 304, 331, 597
BmrFI CCNGG 1 cut(s) 334
BmsI GCATC 1 cut(s) 661
BpmI CTGGAG 1 cut(s) 451
BsaJI CCNNGG 6 cut(s) 136, 160, 297, 577, 667, 678
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 119, 468
BseBI CCWGG 1 cut(s) 334
BseDI CCNNGG 6 cut(s) 136, 160, 297, 577, 667, 678
BseGI GGATG 1 cut(s) 652
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMII CTCAG 4 cut(s) 170, 306, 531, 537
BseNI ACTGG 2 cut(s) 119, 468
BseRI GAGGAG 4 cut(s) 254, 257, 260, 475
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 3 cut(s) 35, 673, 723
Bsh1236I CGCG 1 cut(s) 716
BshFI GGCC 3 cut(s) 65, 575, 597
BshNI GGYRCC 1 cut(s) 266
BsiHKAI GWGCWC 1 cut(s) 467
BsiHKCI CYCGRG 1 cut(s) 578
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 2 cut(s) 377, 499
BsmBI CGTCTC 1 cut(s) 377
BsmI GAATGC 1 cut(s) 628
BsnI GGCC 3 cut(s) 65, 575, 597
BsoBI CYCGRG 1 cut(s) 578
Bsp120I GGGCCC 1 cut(s) 595
Bsp1286I GDGCHC 2 cut(s) 467, 599
Bsp143I GATC 4 cut(s) 94, 146, 322, 395
Bsp19I CCATGG 2 cut(s) 136, 160
BspACI CCGC 5 cut(s) 20, 66, 457, 716, 735
BspANI GGCC 3 cut(s) 65, 575, 597
BspCNI CTCAG 4 cut(s) 171, 305, 532, 538
BspFNI CGCG 1 cut(s) 716
BspLI GGNNCC 6 cut(s) 158, 249, 268, 304, 331, 597
BspMAI CTGCAG 1 cut(s) 691
BspPI GGATC 3 cut(s) 102, 141, 390
BspT107I GGYRCC 1 cut(s) 266
BsrI ACTGG 2 cut(s) 119, 468
BssECI CCNNGG 6 cut(s) 136, 160, 297, 577, 667, 678
BssMI GATC 4 cut(s) 94, 146, 322, 395
BssT1I CCWWGG 5 cut(s) 136, 160, 297, 667, 678
Bst2UI CCWGG 1 cut(s) 334
Bst4CI ACNGT 1 cut(s) 662
Bst6I CTCTTC 2 cut(s) 438, 516
BstC8I GCNNGC 2 cut(s) 171, 588
BstDEI CTNAG 4 cut(s) 179, 292, 540, 546
BstDSI CCRYGG 2 cut(s) 136, 160
BstF5I GGATG 1 cut(s) 652
BstFNI CGCG 1 cut(s) 716
BstHHI GCGC 2 cut(s) 643, 716
BstKTI GATC 4 cut(s) 97, 149, 325, 398
BstMAI GTCTC 2 cut(s) 377, 499
BstMBI GATC 4 cut(s) 94, 146, 322, 395
BstMWI GCNNNNNNNGC 2 cut(s) 17, 649
BstNI CCWGG 1 cut(s) 334
BstSCI CCNGG 1 cut(s) 332
BstSFI CTRYAG 1 cut(s) 687
BstSLI GKGCMC 1 cut(s) 599
BstUI CGCG 1 cut(s) 716
BstV1I GCAGC 3 cut(s) 35, 673, 723
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 3 cut(s) 65, 575, 597
BtgI CCRYGG 2 cut(s) 136, 160
BtsCI GGATG 1 cut(s) 652
Cac8I GCNNGC 2 cut(s) 171, 588
CfoI GCGC 2 cut(s) 643, 716
Cfr13I GGNCC 5 cut(s) 63, 303, 574, 595, 596
CseI GACGC 1 cut(s) 114
CviAII CATG 5 cut(s) 13, 137, 161, 273, 400
DdeI CTNAG 4 cut(s) 179, 292, 540, 546
DpnI GATC 4 cut(s) 96, 148, 324, 397
DpnII GATC 4 cut(s) 94, 146, 322, 395
DraI TTTAAA 1 cut(s) 280
Eam1104I CTCTTC 2 cut(s) 438, 516
EarI CTCTTC 2 cut(s) 438, 516
Ecl136II GAGCTC 1 cut(s) 465
Eco130I CCWWGG 5 cut(s) 136, 160, 297, 667, 678
Eco24I GRGCYC 2 cut(s) 467, 599
Eco47I GGWCC 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 465
Eco88I CYCGRG 1 cut(s) 578
EcoICRI GAGCTC 1 cut(s) 465
EcoO109I RGGNCCY 1 cut(s) 596
EcoRI GAATTC 1 cut(s) 448
EcoRII CCWGG 1 cut(s) 332
EcoT14I CCWWGG 5 cut(s) 136, 160, 297, 667, 678
EcoT22I ATGCAT 1 cut(s) 628
EcoT38I GRGCYC 2 cut(s) 467, 599
ErhI CCWWGG 5 cut(s) 136, 160, 297, 667, 678
Esp3I CGTCTC 1 cut(s) 377
FaeI CATG 5 cut(s) 16, 140, 164, 276, 403
FatI CATG 5 cut(s) 12, 136, 160, 272, 399
Fnu4HI GCNGC 6 cut(s) 21, 49, 66, 687, 712, 717
FokI GGATG 1 cut(s) 639
FriOI GRGCYC 2 cut(s) 467, 599
Fsp4HI GCNGC 6 cut(s) 21, 49, 66, 687, 712, 717
FspBI CTAG 1 cut(s) 123
GlaI GCGC 2 cut(s) 642, 715
GluI GCNGC 6 cut(s) 21, 49, 66, 687, 712, 717
GsuI CTGGAG 1 cut(s) 451
HaeIII GGCC 3 cut(s) 65, 575, 597
HgaI GACGC 1 cut(s) 114
HhaI GCGC 2 cut(s) 643, 716
Hin1II CATG 5 cut(s) 16, 140, 164, 276, 403
Hin6I GCGC 2 cut(s) 641, 714
HinP1I GCGC 2 cut(s) 641, 714
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 4 cut(s) 126, 536, 557, 633
HphI GGTGA 1 cut(s) 656
Hpy166II GTNNAC 1 cut(s) 751
Hpy188I TCNGA 4 cut(s) 216, 395, 541, 562
Hpy188III TCNNGA 3 cut(s) 150, 619, 656
Hpy8I GTNNAC 1 cut(s) 751
Hpy99I CGWCG 1 cut(s) 108
HpyAV CCTTC 2 cut(s) 147, 483
HpyCH4III ACNGT 1 cut(s) 662
HpyCH4V TGCA 5 cut(s) 51, 200, 626, 689, 711
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 649
HpyF3I CTNAG 4 cut(s) 179, 292, 540, 546
Hsp92II CATG 5 cut(s) 16, 140, 164, 276, 403
HspAI GCGC 2 cut(s) 641, 714
Kzo9I GATC 4 cut(s) 94, 146, 322, 395
LmnI GCTCC 6 cut(s) 113, 162, 247, 329, 462, 470
LpnPI CCDG 9 cut(s) 100, 163, 303, 319, 343, 346, 481, 558, 669
Lsp1109I GCAGC 3 cut(s) 35, 673, 723
LweI GCATC 1 cut(s) 661
MaeI CTAG 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 174, 662
MalI GATC 4 cut(s) 96, 148, 324, 397
MboI GATC 4 cut(s) 94, 146, 322, 395
MboII GAAGA 8 cut(s) 153, 156, 357, 377, 380, 455, 533, 666
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 2 cut(s) 467, 599
MluCI AATT 7 cut(s) 337, 416, 448, 472, 499, 550, 740
MlyI GAGTC 2 cut(s) 530, 566
Mph1103I ATGCAT 1 cut(s) 628
MseI TTAA 2 cut(s) 279, 438
MslI CAYNNNNRTG 2 cut(s) 11, 56
MspA1I CMGCKG 1 cut(s) 457
MspR9I CCNGG 1 cut(s) 334
Mva1269I GAATGC 1 cut(s) 628
MvaI CCWGG 1 cut(s) 334
MvnI CGCG 1 cut(s) 716
MwoI GCNNNNNNNGC 2 cut(s) 17, 649
NcoI CCATGG 2 cut(s) 136, 160
NdeII GATC 4 cut(s) 94, 146, 322, 395
NlaIII CATG 5 cut(s) 16, 140, 164, 276, 403
NlaIV GGNNCC 6 cut(s) 158, 249, 268, 304, 331, 597
NmuCI GTSAC 1 cut(s) 662
NsiI ATGCAT 1 cut(s) 628
PctI GAATGC 1 cut(s) 628
PfeI GAWTC 2 cut(s) 126, 633
PkrI GCNGC 6 cut(s) 22, 50, 67, 688, 713, 718
PleI GAGTC 2 cut(s) 530, 565
PpsI GAGTC 2 cut(s) 530, 565
PshBI ATTAAT 1 cut(s) 438
Psp124BI GAGCTC 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 332
PspGI CCWGG 1 cut(s) 332
PspN4I GGNNCC 6 cut(s) 158, 249, 268, 304, 331, 597
PspOMI GGGCCC 1 cut(s) 595
PspPI GGNCC 5 cut(s) 63, 303, 574, 595, 596
PstI CTGCAG 1 cut(s) 691
PsuI RGATCY 1 cut(s) 146
RseI CAYNNNNRTG 2 cut(s) 11, 56
SacI GAGCTC 1 cut(s) 467
SaqAI TTAA 2 cut(s) 279, 438
SatI GCNGC 6 cut(s) 21, 49, 66, 687, 712, 717
Sau3AI GATC 4 cut(s) 94, 146, 322, 395
Sau96I GGNCC 5 cut(s) 63, 303, 574, 595, 596
SchI GAGTC 2 cut(s) 530, 566
ScrFI CCNGG 1 cut(s) 334
SduI GDGCHC 2 cut(s) 467, 599
SetI ASST 8 cut(s) 124, 272, 467, 494, 572, 673, 684, 708
SfaNI GCATC 1 cut(s) 661
SfcI CTRYAG 1 cut(s) 687
SinI GGWCC 1 cut(s) 303
SmiMI CAYNNNNRTG 2 cut(s) 11, 56
Sse9I AATT 7 cut(s) 337, 416, 448, 472, 499, 550, 740
SsiI CCGC 5 cut(s) 20, 66, 457, 716, 735
SspMI CTAG 1 cut(s) 123
SstI GAGCTC 1 cut(s) 467
StyD4I CCNGG 1 cut(s) 332
StyI CCWWGG 5 cut(s) 136, 160, 297, 667, 678
TaaI ACNGT 1 cut(s) 662
TaqI TCGA 1 cut(s) 631
TasI AATT 7 cut(s) 337, 416, 448, 472, 499, 550, 740
TauI GCSGC 3 cut(s) 23, 68, 719
TfiI GAWTC 2 cut(s) 126, 633
Tru1I TTAA 2 cut(s) 279, 438
Tru9I TTAA 2 cut(s) 279, 438
TseFI GTSAC 1 cut(s) 662
TseI GCWGC 3 cut(s) 48, 686, 711
Tsp45I GTSAC 1 cut(s) 662
TspDTI ATGAA 6 cut(s) 17, 29, 65, 424, 456, 579
TspGWI ACGGA 1 cut(s) 357
VpaK11BI GGWCC 1 cut(s) 303
VspI ATTAAT 1 cut(s) 438
XapI RAATTY 3 cut(s) 416, 448, 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 1 cut(s) 123
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.