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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 364 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 331 |
| AgsI | TTSAA | 2 cut(s) | 124, 302 |
| AluBI | AGCT | 2 cut(s) | 386, 395 |
| AluI | AGCT | 2 cut(s) | 386, 395 |
| AoxI | GGCC | 1 cut(s) | 107 |
| ApeKI | GCWGC | 2 cut(s) | 44, 386 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 81 |
| AsuHPI | GGTGA | 2 cut(s) | 80, 371 |
| BauI | CACGAG | 1 cut(s) | 48 |
| BbvI | GCAGC | 2 cut(s) | 31, 373 |
| BciVI | GTATCC | 1 cut(s) | 49 |
| BfuI | GTATCC | 1 cut(s) | 49 |
| BisI | GCNGC | 2 cut(s) | 45, 387 |
| BlsI | GCNGC | 2 cut(s) | 46, 388 |
| BmsI | GCATC | 1 cut(s) | 166 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 238, 270 |
| Bpu10I | CCTNAGC | 1 cut(s) | 255 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 331 |
| Bse1I | ACTGG | 1 cut(s) | 64 |
| BseGI | GGATG | 1 cut(s) | 133 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 331 |
| BseMII | CTCAG | 1 cut(s) | 269 |
| BseNI | ACTGG | 1 cut(s) | 64 |
| BseXI | GCAGC | 2 cut(s) | 31, 373 |
| BshFI | GGCC | 1 cut(s) | 109 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 331 |
| BsnI | GGCC | 1 cut(s) | 109 |
| BspACI | CCGC | 1 cut(s) | 364 |
| BspANI | GGCC | 1 cut(s) | 109 |
| BspCNI | CTCAG | 1 cut(s) | 268 |
| BsrI | ACTGG | 1 cut(s) | 64 |
| BssSI | CACGAG | 1 cut(s) | 48 |
| Bst2BI | CACGAG | 1 cut(s) | 48 |
| Bst6I | CTCTTC | 3 cut(s) | 66, 94, 239 |
| BstDEI | CTNAG | 1 cut(s) | 255 |
| BstF5I | GGATG | 1 cut(s) | 133 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 392 |
| BstV1I | GCAGC | 2 cut(s) | 31, 373 |
| BsuI | GTATCC | 1 cut(s) | 49 |
| BsuRI | GGCC | 1 cut(s) | 109 |
| BtsCI | GGATG | 1 cut(s) | 133 |
| CviAII | CATG | 1 cut(s) | 205 |
| CviJI | RGCY | 7 cut(s) | 109, 152, 254, 335, 376, 386, 395 |
| CviKI_1 | RGCY | 7 cut(s) | 109, 152, 254, 335, 376, 386, 395 |
| DdeI | CTNAG | 1 cut(s) | 255 |
| DraI | TTTAAA | 1 cut(s) | 156 |
| Eam1104I | CTCTTC | 3 cut(s) | 66, 94, 239 |
| EarI | CTCTTC | 3 cut(s) | 66, 94, 239 |
| EciI | GGCGGA | 1 cut(s) | 379 |
| FaeI | CATG | 1 cut(s) | 208 |
| FaiI | YATR | 3 cut(s) | 206, 224, 272 |
| FatI | CATG | 1 cut(s) | 204 |
| Fnu4HI | GCNGC | 2 cut(s) | 45, 387 |
| FokI | GGATG | 1 cut(s) | 140 |
| Fsp4HI | GCNGC | 2 cut(s) | 45, 387 |
| GluI | GCNGC | 2 cut(s) | 45, 387 |
| HaeIII | GGCC | 1 cut(s) | 109 |
| Hin1II | CATG | 1 cut(s) | 208 |
| HinfI | GANTC | 2 cut(s) | 82, 342 |
| HphI | GGTGA | 2 cut(s) | 80, 371 |
| Hpy188I | TCNGA | 1 cut(s) | 347 |
| Hpy188III | TCNNGA | 2 cut(s) | 50, 317 |
| HpyAV | CCTTC | 4 cut(s) | 99, 118, 134, 228 |
| HpyCH4V | TGCA | 2 cut(s) | 179, 408 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 392 |
| HpyF3I | CTNAG | 1 cut(s) | 255 |
| Hsp92II | CATG | 1 cut(s) | 208 |
| LpnPI | CCDG | 4 cut(s) | 45, 79, 242, 381 |
| Lsp1109I | GCAGC | 2 cut(s) | 31, 373 |
| LweI | GCATC | 1 cut(s) | 166 |
| MboII | GAAGA | 4 cut(s) | 83, 111, 176, 256 |
| MmeI | TCCRAC | 2 cut(s) | 319, 370 |
| MnlI | CCTC | 7 cut(s) | 8, 46, 67, 95, 240, 280, 387 |
| MroXI | GAANNNNTTC | 1 cut(s) | 81 |
| MseI | TTAA | 2 cut(s) | 155, 261 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 392 |
| NlaIII | CATG | 1 cut(s) | 208 |
| PdmI | GAANNNNTTC | 1 cut(s) | 81 |
| PfeI | GAWTC | 2 cut(s) | 82, 342 |
| PkrI | GCNGC | 2 cut(s) | 46, 388 |
| SaqAI | TTAA | 2 cut(s) | 155, 261 |
| SatI | GCNGC | 2 cut(s) | 45, 387 |
| SetI | ASST | 6 cut(s) | 13, 187, 251, 261, 388, 397 |
| SfaNI | GCATC | 1 cut(s) | 166 |
| SsiI | CCGC | 1 cut(s) | 364 |
| TaqI | TCGA | 1 cut(s) | 171 |
| TfiI | GAWTC | 2 cut(s) | 82, 342 |
| Tru1I | TTAA | 2 cut(s) | 155, 261 |
| Tru9I | TTAA | 2 cut(s) | 155, 261 |
| TseI | GCWGC | 2 cut(s) | 44, 386 |
| TspDTI | ATGAA | 1 cut(s) | 221 |
| TspGWI | ACGGA | 1 cut(s) | 246 |
| XmnI | GAANNNNTTC | 1 cut(s) | 81 |