pycom14g06550

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002172
Physical Location & Seq
Forward (+)
6487630 .. 6489095
1466 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1335 bp
ATGTTTATGCATGGCTTAAACCGTTACAAGGAGAAACCTCCTCGAACTTTTTTGGTGTTTATATGGGAAGCACTACAGGACTTAACATTAATGATCCTTATGGTCTGTGCTGTGGTTTCAATTGGAGTTGGAATTGCCACTGAAGGATGGCCCAAAGTGGTAACAGTTACCGCCATTAGTAACTACAAGCAGCCTTCGCAATTCAAGAATTTGGACAGGGAGAAGAAAAAGATTTTTGTTCAGGTCACTAGGGATGGAAAAAGACAAAAAGTTTCCATTTACGACTTGGTTGTTGGAGATATTGTGCATTTGTTGGTTGGGGATGTAGTTCCAGCTGATGGACTTTTCATATCAGGGTACAGTTTGCTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCGGTGAATATATGTGAAGAGAAGCCATTTTTTCTTACCGGAACTACAGTGCAGGATGGATCAGGTAAAATGCTCGTGACTGCAGTTGGTATGAGGACTGAATGGGGAAAATTGATGGAAACGCTTAGTGAAGGAGGAGAAGATGAGACCCCACAGCAGGTGAAGCTTAATGGTGTTGCTACAATTACTGGTAAAATTGGTATGGCTTTTGCAGTGTTGACATTTTTGGTATTGACAGTAAGATTTTTGGTGACAAAAGGACTTAACAACGAGATCACTGATTGGTCTTCAAATAATGCCGTAACGCTTTTGAACTACTTTGCTATTGCGGTAACTATAATTGTTGTTGCAGTTCCCGAAGGATTACCAATGGCAGTGACGGCACTTGCAAGGAATCTCTCAGCATGTGAGACAATGGGTTCTGCTGGTTGTATTTGCACAGATAAAACCGAACCCGGAACATTAACTACAAATCATATAGTAGTTACCAAAGTATGGATATGTGAAAAATCTGTAGATGCAGAAGAAAATGACAGTAAAGAAACATTGACATCGGAAATATATGGAGCATCAAGCATCCTTTTGCAGGTTATATTTCAAAGTACCAGCTCTGAGGTTACTAAGGATGATGGAAAGACCTCCATTTTGGGAACACCAGCGGAGTCAGCATTGCTAGAGTTCGGTTTACTTTTAGGTGGCGATTTTGATGCCCTGCGCCGAGAGGTAAAGATTCTTAAGATAGAACCTTTTAATTCTGTCAGGAAGAAAATGTCTGTTCTTGTAGCTCATCCTCATGGTGGAAAACGAGCGTTTTGCAAAGGTGCATCAGAAATAGTACTGGGAATATGTAACAAGTACATCGACTCTAATGGAGAATCTGTTCATCTCCCCGAAGAAATGGTAAAGAATATCACGAATCTTTCGAGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

445

Amino Acids

48.29

Weight (kDa)

5.47

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 4 - 40 4.2e-07 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 79 - 175 4.4e-22 P-type ATPase actuator domain
Cation_ATPase PF13246 337 - 441 2.1e-19 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 550
Acc36I ACCTGC 2 cut(s) 550, 979
AccB7I CCANNNNNTGG 2 cut(s) 338, 897
AciI CCGC 3 cut(s) 171, 731, 1061
AclWI GGATC 2 cut(s) 88, 469
AcsI RAATTY 1 cut(s) 208
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 4 cut(s) 359, 1006, 1239, 1259
AfiI CCNNNNNNNGG 6 cut(s) 28, 338, 559, 897, 988, 1199
AflII CTTAAG 1 cut(s) 1136
AgsI TTSAA 5 cut(s) 120, 205, 693, 715, 1001
AloI GAACNNNNNNTCC 2 cut(s) 1266, 1298
AluBI AGCT 5 cut(s) 335, 384, 568, 1011, 1187
AluI AGCT 5 cut(s) 335, 384, 568, 1011, 1187
Alw26I GTCTC 2 cut(s) 542, 806
AlwI GGATC 2 cut(s) 88, 469
AoxI GGCC 1 cut(s) 149
ApeKI GCWGC 1 cut(s) 190
ApoI RAATTY 1 cut(s) 208
ArsI GACNNNNNNTTYG 2 cut(s) 1030, 1062
AseI ATTAAT 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 1281
AspLEI GCGC 1 cut(s) 1119
AspS9I GGNCC 1 cut(s) 150
AsuC2I CCSGG 1 cut(s) 858
AsuHPI GGTGA 4 cut(s) 404, 418, 574, 664
BauI CACGAG 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 681
BbvI GCAGC 1 cut(s) 202
BccI CCATC 6 cut(s) 141, 248, 332, 452, 511, 1025
BceAI ACGGC 2 cut(s) 686, 798
BcgI CGANNNNNNTGC 4 cut(s) 1091, 1125, 1197, 1231
BcnI CCSGG 1 cut(s) 858
BcoDI GTCTC 2 cut(s) 542, 806
BfaI CTAG 2 cut(s) 249, 1076
BfmI CTRYAG 4 cut(s) 74, 447, 483, 915
BfrI CTTAAG 1 cut(s) 1136
BfuAI ACCTGC 2 cut(s) 550, 979
BisI GCNGC 1 cut(s) 191
BlsI GCNGC 1 cut(s) 192
BmcAI AGTACT 1 cut(s) 1239
Bme1390I CCNGG 1 cut(s) 858
BmgT120I GGNCC 1 cut(s) 150
BmrFI CCNGG 1 cut(s) 858
BmrI ACTGGG 1 cut(s) 1250
BmsI GCATC 5 cut(s) 910, 980, 987, 1099, 1235
BmuI ACTGGG 1 cut(s) 1250
BpiI GAAGAC 1 cut(s) 681
BpuMI CCSGG 1 cut(s) 858
BsaI GGTCTC 1 cut(s) 542
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 1266, 1296
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 338, 559, 897, 988, 1199
Bse118I RCCGGY 1 cut(s) 403
Bse1I ACTGG 2 cut(s) 595, 1245
Bse3DI GCAATG 1 cut(s) 1070
BseGI GGATG 7 cut(s) 152, 259, 328, 463, 978, 1033, 1189
BseLI CCNNNNNNNGG 6 cut(s) 28, 338, 559, 897, 988, 1199
BseMI GCAATG 1 cut(s) 1070
BseMII CTCAG 2 cut(s) 816, 1005
BseNI ACTGG 2 cut(s) 595, 1245
BseRI GAGGAG 2 cut(s) 30, 552
BseXI GCAGC 1 cut(s) 202
BsgI GTGCAG 1 cut(s) 473
BshFI GGCC 1 cut(s) 151
BsiSI CCGG 3 cut(s) 404, 441, 858
BslI CCNNNNNNNGG 6 cut(s) 28, 338, 559, 897, 988, 1199
BsmAI GTCTC 2 cut(s) 542, 806
BsnI GGCC 1 cut(s) 151
Bso31I GGTCTC 1 cut(s) 542
Bsp143I GATC 3 cut(s) 93, 461, 675
BspACI CCGC 3 cut(s) 171, 731, 1061
BspANI GGCC 1 cut(s) 151
BspCNI CTCAG 2 cut(s) 815, 1006
BspMAI CTGCAG 1 cut(s) 487
BspMI ACCTGC 2 cut(s) 550, 979
BspPI GGATC 2 cut(s) 88, 469
BspTI CTTAAG 1 cut(s) 1136
BspTNI GGTCTC 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 1070
BsrFI RCCGGY 1 cut(s) 403
BsrI ACTGG 2 cut(s) 595, 1245
BssAI RCCGGY 1 cut(s) 403
BssMI GATC 3 cut(s) 93, 461, 675
BssSI CACGAG 1 cut(s) 476
Bst2BI CACGAG 1 cut(s) 476
Bst4CI ACNGT 6 cut(s) 23, 166, 362, 451, 640, 938
Bst6I CTCTTC 1 cut(s) 414
BstAFI CTTAAG 1 cut(s) 1136
BstDEI CTNAG 4 cut(s) 527, 802, 1014, 1023
BstENI CCTNNNNNAGG 1 cut(s) 986
BstF5I GGATG 7 cut(s) 152, 259, 328, 463, 978, 1033, 1189
BstHHI GCGC 1 cut(s) 1119
BstKTI GATC 3 cut(s) 96, 464, 678
BstMAI GTCTC 2 cut(s) 542, 806
BstMBI GATC 3 cut(s) 93, 461, 675
BstMWI GCNNNNNNNGC 4 cut(s) 196, 565, 782, 1067
BstNSI RCATGY 1 cut(s) 810
BstSCI CCNGG 1 cut(s) 856
BstSFI CTRYAG 4 cut(s) 74, 447, 483, 915
BstV1I GCAGC 1 cut(s) 202
BstV2I GAAGAC 1 cut(s) 681
BsuRI GGCC 1 cut(s) 151
BtsCI GGATG 7 cut(s) 152, 259, 328, 463, 978, 1033, 1189
BtsI GCAGTG 2 cut(s) 621, 783
BtsIMutI CAGTG 5 cut(s) 138, 456, 621, 678, 783
BveI ACCTGC 2 cut(s) 550, 979
CfoI GCGC 1 cut(s) 1119
Cfr10I RCCGGY 1 cut(s) 403
Cfr13I GGNCC 1 cut(s) 150
Csp6I GTAC 4 cut(s) 358, 1005, 1238, 1258
CviAII CATG 3 cut(s) 11, 807, 1196
CviQI GTAC 4 cut(s) 358, 1005, 1238, 1258
DdeI CTNAG 4 cut(s) 527, 802, 1014, 1023
DpnI GATC 3 cut(s) 95, 463, 677
DpnII GATC 3 cut(s) 93, 461, 675
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco31I GGTCTC 1 cut(s) 542
Eco57I CTGAAG 1 cut(s) 162
EcoNI CCTNNNNNAGG 1 cut(s) 986
EcoT22I ATGCAT 1 cut(s) 12
FaeI CATG 3 cut(s) 14, 810, 1199
FatI CATG 3 cut(s) 10, 806, 1195
Fnu4HI GCNGC 1 cut(s) 191
FokI GGATG 7 cut(s) 159, 266, 335, 470, 965, 1040, 1176
Fsp4HI GCNGC 1 cut(s) 191
FspBI CTAG 2 cut(s) 249, 1076
GlaI GCGC 1 cut(s) 1118
GluI GCNGC 1 cut(s) 191
HaeIII GGCC 1 cut(s) 151
HapII CCGG 3 cut(s) 404, 441, 858
HhaI GCGC 1 cut(s) 1119
Hin1II CATG 3 cut(s) 14, 810, 1199
Hin6I GCGC 1 cut(s) 1117
HinP1I GCGC 1 cut(s) 1117
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HindIII AAGCTT 2 cut(s) 382, 566
HinfI GANTC 7 cut(s) 377, 796, 1064, 1132, 1265, 1277, 1318
HpaII CCGG 3 cut(s) 404, 441, 858
HphI GGTGA 4 cut(s) 404, 418, 574, 664
Hpy166II GTNNAC 2 cut(s) 621, 1088
Hpy188I TCNGA 3 cut(s) 958, 1015, 1231
Hpy188III TCNNGA 5 cut(s) 205, 478, 758, 1162, 1315
Hpy8I GTNNAC 2 cut(s) 621, 1088
HpyAV CCTTC 4 cut(s) 137, 204, 527, 755
HpyCH4III ACNGT 6 cut(s) 23, 166, 362, 451, 640, 938
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 565, 782, 1067
HpyF3I CTNAG 4 cut(s) 527, 802, 1014, 1023
Hsp92II CATG 3 cut(s) 14, 810, 1199
HspAI GCGC 1 cut(s) 1117
Kzo9I GATC 3 cut(s) 93, 461, 675
LmnI GCTCC 1 cut(s) 968
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 5 cut(s) 910, 980, 987, 1099, 1235
MaeI CTAG 2 cut(s) 249, 1076
MalI GATC 3 cut(s) 95, 463, 677
MboI GATC 3 cut(s) 93, 461, 675
MboII GAAGA 7 cut(s) 235, 431, 554, 681, 938, 1177, 1307
MfeI CAATTG 1 cut(s) 120
MluCI AATT 9 cut(s) 120, 132, 200, 208, 512, 585, 597, 741, 1153
MlyI GAGTC 3 cut(s) 386, 1073, 1259
MmeI TCCRAC 2 cut(s) 109, 274
MnlI CCTC 9 cut(s) 48, 51, 489, 530, 1009, 1051, 1117, 1203, 1320
Mph1103I ATGCAT 1 cut(s) 12
MroXI GAANNNNTTC 1 cut(s) 1281
MseI TTAA 8 cut(s) 17, 83, 89, 570, 666, 866, 1137, 1152
MslI CAYNNNNRTG 1 cut(s) 1194
MspA1I CMGCKG 2 cut(s) 335, 1061
MspCI CTTAAG 1 cut(s) 1136
MspI CCGG 3 cut(s) 404, 441, 858
MspR9I CCNGG 1 cut(s) 858
MunI CAATTG 1 cut(s) 120
MwoI GCNNNNNNNGC 4 cut(s) 196, 565, 782, 1067
NciI CCSGG 1 cut(s) 858
NdeII GATC 3 cut(s) 93, 461, 675
NlaIII CATG 3 cut(s) 14, 810, 1199
NmeAIII GCCGAG 1 cut(s) 1145
NmuCI GTSAC 4 cut(s) 244, 478, 652, 778
NsiI ATGCAT 1 cut(s) 12
NspI RCATGY 1 cut(s) 810
PaqCI CACCTGC 1 cut(s) 550
PcsI WCGNNNNNNNCGW 1 cut(s) 1322
PdmI GAANNNNTTC 1 cut(s) 1281
PfeI GAWTC 4 cut(s) 796, 1132, 1277, 1318
PflMI CCANNNNNTGG 2 cut(s) 338, 897
PkrI GCNGC 1 cut(s) 192
PleI GAGTC 3 cut(s) 385, 1072, 1259
PpsI GAGTC 3 cut(s) 385, 1072, 1259
PshBI ATTAAT 1 cut(s) 89
PspPI GGNCC 1 cut(s) 150
PsrI GAACNNNNNNTAC 2 cut(s) 853, 885
PstI CTGCAG 1 cut(s) 487
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 4 cut(s) 359, 1006, 1239, 1259
RsaNI GTAC 4 cut(s) 358, 1005, 1238, 1258
RseI CAYNNNNRTG 1 cut(s) 1194
SaqAI TTAA 8 cut(s) 17, 83, 89, 570, 666, 866, 1137, 1152
SatI GCNGC 1 cut(s) 191
Sau3AI GATC 3 cut(s) 93, 461, 675
Sau96I GGNCC 1 cut(s) 150
ScaI AGTACT 1 cut(s) 1239
SchI GAGTC 3 cut(s) 386, 1073, 1259
ScrFI CCNGG 1 cut(s) 858
SfaNI GCATC 5 cut(s) 910, 980, 987, 1099, 1235
SfcI CTRYAG 4 cut(s) 74, 447, 483, 915
SmiMI CAYNNNNRTG 1 cut(s) 1194
SmlI CTYRAG 1 cut(s) 1136
SmoI CTYRAG 1 cut(s) 1136
Sse9I AATT 9 cut(s) 120, 132, 200, 208, 512, 585, 597, 741, 1153
SsiI CCGC 3 cut(s) 171, 731, 1061
SspMI CTAG 2 cut(s) 249, 1076
StyD4I CCNGG 1 cut(s) 856
TaaI ACNGT 6 cut(s) 23, 166, 362, 451, 640, 938
TaqI TCGA 3 cut(s) 43, 1263, 1325
TasI AATT 9 cut(s) 120, 132, 200, 208, 512, 585, 597, 741, 1153
TatI WGTACW 2 cut(s) 1237, 1257
TfiI GAWTC 4 cut(s) 796, 1132, 1277, 1318
Tru1I TTAA 8 cut(s) 17, 83, 89, 570, 666, 866, 1137, 1152
Tru9I TTAA 8 cut(s) 17, 83, 89, 570, 666, 866, 1137, 1152
TscAI CASTG 5 cut(s) 145, 456, 621, 685, 783
TseFI GTSAC 4 cut(s) 244, 478, 652, 778
TseI GCWGC 1 cut(s) 190
Tsp45I GTSAC 4 cut(s) 244, 478, 652, 778
TspDTI ATGAA 2 cut(s) 337, 1274
TspRI CASTG 5 cut(s) 145, 456, 621, 685, 783
Van91I CCANNNNNTGG 2 cut(s) 338, 897
Vha464I CTTAAG 1 cut(s) 1136
VspI ATTAAT 1 cut(s) 89
XagI CCTNNNNNAGG 1 cut(s) 986
XapI RAATTY 1 cut(s) 208
XceI RCATGY 1 cut(s) 810
XcmI CCANNNNNNNNNTGG 1 cut(s) 283
XmnI GAANNNNTTC 1 cut(s) 1281
XspI CTAG 2 cut(s) 249, 1076
ZrmI AGTACT 1 cut(s) 1239
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.