pycom14g09940

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005616
Physical Location & Seq
Reverse (-)
12259898 .. 12263843
3946 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
ATGGATGGGAGGGAAGCAATGGGATTGTCTGGTGGGTCAGCTTCATACTACATCCATAGGGGAGGAGGGGTTGGTGGGTCTATGCCTGGATCACAGGCTACTGGCGGTGGCGGCGGACTTCATCATGCTCCACCTGGGTTCAGGCCCATCTCAAACACACTCATGCAACCTCAGTCCAATGTGAGGGTCAGCTCTGCGGGATCAACATTCTCAGTAGAGCCTTCAAGACCCAATTTTCAACATCGTGGCAGTACCATGAATGTCGCTCCCGGAGTGCCTTCAGCTCAGCCTGTAAAGAAGAAAAGAGGGAGGCCCCGGAAGTATGGCCCCGATGGGCCGGTTTCTCTGGGGTTGTCTCCCATGTCTGCAACACCTACTCCTACACCGGGTTCAACCAGTCCGACCCCGAAACGTAGTAGAGGGAGGCCACCTGGGAGTGGTAGGAAGCAACAGTTGGCTACTCTTGGGGATTGGATGAACACTTCAGCCGGATTAGCTTTTGCACCCCATGTTATCACCATTGGAGCTGGAGAGGACATTGCAGCAAAATTATTGTTGTTTGCACAACAGAGACCAAGGGCGCTATGCATCTTGTCAGGAAATGGTGCAGTGTCTTCAGTAACTCTACGTGAGCCTGCATCTACTGGTGTCAGTGTCACATATGAGGGTCGGTTCCAGATATTATGCTTGTCAGGTTCTTACTTAGTTGCAGAGGATGGTGGACCTCGCAATCGAACGGGGGGAATAAGCGTTTCCCTTTCTAATCCTAATGGTCATGTCATTGGTGGTGCTGTGGCAGTGCTGATTGCAGCGACTCCAGTGCAGGTTGTGCTTTGCAGTTTCGTATATGGTGGCTCCAAGACCAAGAACAAGCAACTGGCTGATCCCAATAGCGATGAGAATTCCGAGCCACAGCATAGCGAGAAATTGGGTCTACCAAGCAGTGCCCCACCAACTCAAAATTATAATCCCTCCGGAGCAGGCATATGGCCTGGCTCGCGGCAAGTTGATTTGAGAAATGCCCACACCGGTATTGACTTGACTCGAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0043621
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

351

Amino Acids

35.93

Weight (kDa)

10.18

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 169 - 281 5.9e-23 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 966
Acc36I ACCTGC 1 cut(s) 814
AccI GTMKAC 1 cut(s) 934
AccII CGCG 1 cut(s) 1000
AccIII TCCGGA 1 cut(s) 974
AciI CCGC 5 cut(s) 105, 111, 114, 197, 1000
AclWI GGATC 3 cut(s) 97, 208, 878
AcsI RAATTY 1 cut(s) 901
AcuI CTGAAG 3 cut(s) 264, 468, 600
AfaI GTAC 1 cut(s) 253
AfiI CCNNNNNNNGG 3 cut(s) 183, 386, 437
AgeI ACCGGT 1 cut(s) 1028
AgsI TTSAA 3 cut(s) 225, 239, 393
AjnI CCWGG 4 cut(s) 85, 133, 430, 991
AjuI GAANNNNNNNTTGG 2 cut(s) 437, 469
AluBI AGCT 5 cut(s) 41, 192, 284, 497, 527
AluI AGCT 5 cut(s) 41, 192, 284, 497, 527
Alw26I GTCTC 2 cut(s) 360, 565
AlwI GGATC 3 cut(s) 97, 208, 878
Ama87I CYCGRG 1 cut(s) 1044
Aor13HI TCCGGA 1 cut(s) 974
AoxI GGCC 6 cut(s) 143, 311, 325, 335, 425, 989
ApeKI GCWGC 2 cut(s) 542, 809
ApoI RAATTY 1 cut(s) 901
AsiGI ACCGGT 1 cut(s) 1028
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 5 cut(s) 144, 312, 326, 335, 722
AsuC2I CCSGG 3 cut(s) 270, 316, 387
AsuHPI GGTGA 1 cut(s) 508
AvaI CYCGRG 1 cut(s) 1044
AvaII GGWCC 1 cut(s) 722
BaeGI GKGCMC 1 cut(s) 949
BbsI GAAGAC 1 cut(s) 606
BbvI GCAGC 2 cut(s) 554, 821
BccI CCATC 3 cut(s) 155, 326, 710
BcgI CGANNNNNNTGC 2 cut(s) 802, 836
BciT130I CCWGG 4 cut(s) 87, 135, 432, 993
BcnI CCSGG 3 cut(s) 270, 316, 387
BcoDI GTCTC 2 cut(s) 360, 565
BfoI RGCGCY 1 cut(s) 584
BfuAI ACCTGC 1 cut(s) 814
BisI GCNGC 4 cut(s) 112, 543, 810, 1001
BlpI GCTNAGC 1 cut(s) 285
BlsI GCNGC 4 cut(s) 113, 544, 811, 1002
Bme1390I CCNGG 7 cut(s) 87, 135, 270, 316, 387, 432, 993
Bme18I GGWCC 1 cut(s) 722
BmeT110I CYCGRG 1 cut(s) 1044
BmgT120I GGNCC 5 cut(s) 144, 312, 326, 335, 722
BmiI GGNNCC 4 cut(s) 314, 328, 674, 856
BmrFI CCNGG 7 cut(s) 87, 135, 270, 316, 387, 432, 993
BmsI GCATC 2 cut(s) 597, 647
BpiI GAAGAC 1 cut(s) 606
BpmI CTGGAG 2 cut(s) 549, 801
Bpu1102I GCTNAGC 1 cut(s) 285
BpuMI CCSGG 3 cut(s) 270, 316, 387
BsaAI YACGTR 1 cut(s) 629
BsaI GGTCTC 1 cut(s) 565
BsaJI CCNNGG 4 cut(s) 134, 314, 431, 575
BsaWI WCCGGW 2 cut(s) 974, 1028
BsaXI ACNNNNNCTCC 4 cut(s) 54, 84, 361, 391
Bsc4I CCNNNNNNNGG 3 cut(s) 183, 386, 437
Bse118I RCCGGY 2 cut(s) 337, 1028
Bse1I ACTGG 5 cut(s) 106, 396, 649, 818, 882
Bse3DI GCAATG 2 cut(s) 24, 537
BseAI TCCGGA 1 cut(s) 974
BseBI CCWGG 4 cut(s) 87, 135, 432, 993
BseDI CCNNGG 4 cut(s) 134, 314, 431, 575
BseGI GGATG 4 cut(s) 10, 51, 480, 721
BseLI CCNNNNNNNGG 3 cut(s) 183, 386, 437
BseMI GCAATG 2 cut(s) 24, 537
BseMII CTCAG 3 cut(s) 185, 225, 299
BseNI ACTGG 5 cut(s) 106, 396, 649, 818, 882
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 949
BseXI GCAGC 2 cut(s) 554, 821
BsgI GTGCAG 2 cut(s) 627, 842
Bsh1236I CGCG 1 cut(s) 1000
BshFI GGCC 6 cut(s) 145, 313, 327, 337, 427, 991
BshTI ACCGGT 1 cut(s) 1028
BsiHKCI CYCGRG 1 cut(s) 1044
BsiSI CCGG 7 cut(s) 270, 316, 338, 386, 489, 975, 1029
BslI CCNNNNNNNGG 3 cut(s) 183, 386, 437
BsmAI GTCTC 2 cut(s) 360, 565
BsnI GGCC 6 cut(s) 145, 313, 327, 337, 427, 991
Bso31I GGTCTC 1 cut(s) 565
BsoBI CYCGRG 1 cut(s) 1044
Bsp1286I GDGCHC 1 cut(s) 949
Bsp13I TCCGGA 1 cut(s) 974
Bsp143I GATC 3 cut(s) 89, 200, 883
Bsp1720I GCTNAGC 1 cut(s) 285
BspACI CCGC 5 cut(s) 105, 111, 114, 197, 1000
BspANI GGCC 6 cut(s) 145, 313, 327, 337, 427, 991
BspCNI CTCAG 3 cut(s) 184, 224, 298
BspEI TCCGGA 1 cut(s) 974
BspFNI CGCG 1 cut(s) 1000
BspLI GGNNCC 4 cut(s) 314, 328, 674, 856
BspMI ACCTGC 1 cut(s) 814
BspPI GGATC 3 cut(s) 97, 208, 878
BspTNI GGTCTC 1 cut(s) 565
BsrDI GCAATG 2 cut(s) 24, 537
BsrFI RCCGGY 2 cut(s) 337, 1028
BsrI ACTGG 5 cut(s) 106, 396, 649, 818, 882
BssAI RCCGGY 2 cut(s) 337, 1028
BssECI CCNNGG 4 cut(s) 134, 314, 431, 575
BssMI GATC 3 cut(s) 89, 200, 883
BssT1I CCWWGG 1 cut(s) 575
Bst2UI CCWGG 4 cut(s) 87, 135, 432, 993
Bst4CI ACNGT 1 cut(s) 453
BstAPI GCANNNNNTGC 1 cut(s) 829
BstBAI YACGTR 1 cut(s) 629
BstC8I GCNNGC 3 cut(s) 636, 982, 998
BstDEI CTNAG 4 cut(s) 171, 211, 285, 703
BstF5I GGATG 4 cut(s) 10, 51, 480, 721
BstFNI CGCG 1 cut(s) 1000
BstH2I RGCGCY 1 cut(s) 584
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 3 cut(s) 92, 203, 886
BstMAI GTCTC 2 cut(s) 360, 565
BstMBI GATC 3 cut(s) 89, 200, 883
BstMWI GCNNNNNNNGC 4 cut(s) 111, 494, 829, 997
BstNI CCWGG 4 cut(s) 87, 135, 432, 993
BstSCI CCNGG 7 cut(s) 85, 133, 268, 314, 385, 430, 991
BstSLI GKGCMC 1 cut(s) 949
BstUI CGCG 1 cut(s) 1000
BstV1I GCAGC 2 cut(s) 554, 821
BstV2I GAAGAC 1 cut(s) 606
BsuRI GGCC 6 cut(s) 145, 313, 327, 337, 427, 991
BtgZI GCGATG 1 cut(s) 909
BtsCI GGATG 4 cut(s) 10, 51, 480, 721
BtsI GCAGTG 3 cut(s) 615, 804, 949
BtsIMutI CAGTG 5 cut(s) 615, 658, 804, 825, 949
BveI ACCTGC 1 cut(s) 814
Cac8I GCNNGC 3 cut(s) 636, 982, 998
CfoI GCGC 1 cut(s) 583
Cfr10I RCCGGY 2 cut(s) 337, 1028
Cfr13I GGNCC 5 cut(s) 144, 312, 326, 335, 722
Csp6I GTAC 1 cut(s) 252
CspAI ACCGGT 1 cut(s) 1028
CviAII CATG 6 cut(s) 125, 163, 256, 361, 509, 776
CviQI GTAC 1 cut(s) 252
DdeI CTNAG 4 cut(s) 171, 211, 285, 703
DpnI GATC 3 cut(s) 91, 202, 885
DpnII GATC 3 cut(s) 89, 200, 883
EciI GGCGGA 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 575
Eco31I GGTCTC 1 cut(s) 565
Eco47I GGWCC 1 cut(s) 722
Eco57I CTGAAG 3 cut(s) 264, 468, 600
Eco88I CYCGRG 1 cut(s) 1044
EcoO109I RGGNCCY 1 cut(s) 312
EcoRI GAATTC 1 cut(s) 901
EcoRII CCWGG 4 cut(s) 85, 133, 430, 991
EcoT14I CCWWGG 1 cut(s) 575
EcoT22I ATGCAT 1 cut(s) 590
ErhI CCWWGG 1 cut(s) 575
FaeI CATG 6 cut(s) 128, 166, 259, 364, 512, 779
FatI CATG 6 cut(s) 124, 162, 255, 360, 508, 775
FauI CCCGC 1 cut(s) 190
FauNDI CATATG 2 cut(s) 661, 986
FblI GTMKAC 1 cut(s) 934
Fnu4HI GCNGC 4 cut(s) 112, 543, 810, 1001
FokI GGATG 4 cut(s) 17, 38, 487, 728
Fsp4HI GCNGC 4 cut(s) 112, 543, 810, 1001
GlaI GCGC 1 cut(s) 582
GluI GCNGC 4 cut(s) 112, 543, 810, 1001
GsuI CTGGAG 2 cut(s) 549, 801
HaeII RGCGCY 1 cut(s) 584
HaeIII GGCC 6 cut(s) 145, 313, 327, 337, 427, 991
HapII CCGG 7 cut(s) 270, 316, 338, 386, 489, 975, 1029
HhaI GCGC 1 cut(s) 583
Hin1II CATG 6 cut(s) 128, 166, 259, 364, 512, 779
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HinfI GANTC 2 cut(s) 814, 1042
HpaII CCGG 7 cut(s) 270, 316, 338, 386, 489, 975, 1029
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 2 cut(s) 722, 935
Hpy188I TCNGA 2 cut(s) 402, 907
Hpy188III TCNNGA 4 cut(s) 225, 597, 676, 975
Hpy8I GTNNAC 2 cut(s) 722, 935
HpyAV CCTTC 2 cut(s) 231, 288
HpyCH4III ACNGT 1 cut(s) 453
HpyCH4IV ACGT 2 cut(s) 412, 628
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 494, 829, 997
HpyF3I CTNAG 4 cut(s) 171, 211, 285, 703
HpySE526I ACGT 2 cut(s) 412, 628
Hsp92II CATG 6 cut(s) 128, 166, 259, 364, 512, 779
HspAI GCGC 1 cut(s) 581
Kpn2I TCCGGA 1 cut(s) 974
Kzo9I GATC 3 cut(s) 89, 200, 883
LmnI GCTCC 5 cut(s) 133, 271, 524, 860, 977
Lsp1109I GCAGC 2 cut(s) 554, 821
LweI GCATC 2 cut(s) 597, 647
MaeII ACGT 2 cut(s) 412, 628
MaeIII GTNAC 2 cut(s) 619, 655
MalI GATC 3 cut(s) 91, 202, 885
MboI GATC 3 cut(s) 89, 200, 883
MboII GAAGA 2 cut(s) 310, 606
MhlI GDGCHC 1 cut(s) 949
MluCI AATT 5 cut(s) 232, 548, 901, 926, 961
MlyI GAGTC 2 cut(s) 808, 1036
MmeI TCCRAC 1 cut(s) 425
Mph1103I ATGCAT 1 cut(s) 590
MroI TCCGGA 1 cut(s) 974
MslI CAYNNNNRTG 1 cut(s) 161
MspI CCGG 7 cut(s) 270, 316, 338, 386, 489, 975, 1029
MspR9I CCNGG 7 cut(s) 87, 135, 270, 316, 387, 432, 993
MvaI CCWGG 4 cut(s) 87, 135, 432, 993
MvnI CGCG 1 cut(s) 1000
MwoI GCNNNNNNNGC 4 cut(s) 111, 494, 829, 997
NciI CCSGG 3 cut(s) 270, 316, 387
NdeI CATATG 2 cut(s) 661, 986
NdeII GATC 3 cut(s) 89, 200, 883
NlaIII CATG 6 cut(s) 128, 166, 259, 364, 512, 779
NlaIV GGNNCC 4 cut(s) 314, 328, 674, 856
NmuCI GTSAC 1 cut(s) 655
NsiI ATGCAT 1 cut(s) 590
PaeR7I CTCGAG 1 cut(s) 1044
PfoI TCCNGGA 1 cut(s) 268
PinAI ACCGGT 1 cut(s) 1028
PkrI GCNGC 4 cut(s) 113, 544, 811, 1002
PleI GAGTC 2 cut(s) 808, 1036
PpsI GAGTC 2 cut(s) 808, 1036
Ppu21I YACGTR 1 cut(s) 629
PsiI TTATAA 1 cut(s) 966
Psp6I CCWGG 4 cut(s) 85, 133, 430, 991
PspGI CCWGG 4 cut(s) 85, 133, 430, 991
PspN4I GGNNCC 4 cut(s) 314, 328, 674, 856
PspPI GGNCC 5 cut(s) 144, 312, 326, 335, 722
PspXI VCTCGAGB 1 cut(s) 1044
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
RseI CAYNNNNRTG 1 cut(s) 161
SatI GCNGC 4 cut(s) 112, 543, 810, 1001
Sau3AI GATC 3 cut(s) 89, 200, 883
Sau96I GGNCC 5 cut(s) 144, 312, 326, 335, 722
SchI GAGTC 2 cut(s) 808, 1036
ScrFI CCNGG 7 cut(s) 87, 135, 270, 316, 387, 432, 993
SduI GDGCHC 1 cut(s) 949
SfaNI GCATC 2 cut(s) 597, 647
Sfr274I CTCGAG 1 cut(s) 1044
SinI GGWCC 1 cut(s) 722
SlaI CTCGAG 1 cut(s) 1044
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 1 cut(s) 1044
SmoI CTYRAG 1 cut(s) 1044
Sse9I AATT 5 cut(s) 232, 548, 901, 926, 961
SsiI CCGC 5 cut(s) 105, 111, 114, 197, 1000
StyD4I CCNGG 7 cut(s) 85, 133, 268, 314, 385, 430, 991
StyI CCWWGG 1 cut(s) 575
TaaI ACNGT 1 cut(s) 453
TaiI ACGT 2 cut(s) 415, 631
TaqI TCGA 2 cut(s) 733, 1045
TasI AATT 5 cut(s) 232, 548, 901, 926, 961
TauI GCSGC 2 cut(s) 114, 1003
TscAI CASTG 5 cut(s) 615, 658, 804, 825, 949
TseFI GTSAC 1 cut(s) 655
TseI GCWGC 2 cut(s) 542, 809
Tsp45I GTSAC 1 cut(s) 655
TspDTI ATGAA 4 cut(s) 33, 110, 272, 491
TspRI CASTG 5 cut(s) 615, 658, 804, 825, 949
VpaK11BI GGWCC 1 cut(s) 722
XapI RAATTY 1 cut(s) 901
XhoI CTCGAG 1 cut(s) 1044
XmiI GTMKAC 1 cut(s) 934
Zsp2I ATGCAT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.