pycom14g15580

NADH-ubiquinone reductase complex 1 MLRQ subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004480
Physical Location & Seq
Reverse (-)
18171268 .. 18172566
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 273 bp
ATGGCTGCGAATCGCTGGCTGAGGCCCGAGGTTTATCCACTGTTCGCGGCCACAGGGGTCGCGATAGGGATCTGCGGGTTGAGTCTGTTCCGCCACATCACCATCAACCCTGAAGTCAGGGTGAGCAAGGAGGGCAGAGCTGCTGGAGTATTGGATAATCACGCCGAAGGAGAAAAGTACAAAGAGAATTTCTTGAGGAAATTTGTCCGCAACAGGGCACCGGAAATTATGCCCGCCATCAACGGTTTCTTCACCGACCCGAAAAATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B12D

Protein Analysis

91

Amino Acids

10.11

Weight (kDa)

9.65

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B12D PF06522 6 - 60 1.1e-20 NADH-ubiquinone reductase complex 1 MLRQ subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AccII CGCG 2 cut(s) 47, 62
AciI CCGC 5 cut(s) 47, 75, 91, 208, 234
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 2 cut(s) 187, 200
AcuI CTGAAG 1 cut(s) 132
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 214
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 2 cut(s) 23, 48
ApeKI GCWGC 2 cut(s) 5, 140
ApoI RAATTY 2 cut(s) 187, 200
AspS9I GGNCC 1 cut(s) 24
AsuHPI GGTGA 3 cut(s) 91, 133, 244
AvaI CYCGRG 1 cut(s) 26
BaeGI GKGCMC 1 cut(s) 220
BanI GGYRCC 1 cut(s) 217
BbvCI CCTCAGC 1 cut(s) 20
BbvI GCAGC 1 cut(s) 127
BccI CCATC 2 cut(s) 110, 245
BisI GCNGC 3 cut(s) 6, 48, 141
BlsI GCNGC 3 cut(s) 7, 49, 142
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 24
BmiI GGNNCC 1 cut(s) 219
BpmI CTGGAG 1 cut(s) 165
Bpu10I CCTNAGC 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 214
BsaBI GATNNNNATC 1 cut(s) 68
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse8I GATNNNNATC 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 27
BseJI GATNNNNATC 1 cut(s) 68
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMII CTCAG 1 cut(s) 11
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 1 cut(s) 127
Bsh1236I CGCG 2 cut(s) 47, 62
BshFI GGCC 2 cut(s) 25, 50
BshNI GGYRCC 1 cut(s) 217
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 1 cut(s) 221
BslI CCNNNNNNNGG 1 cut(s) 214
BsnI GGCC 2 cut(s) 25, 50
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 1 cut(s) 69
Bsp68I TCGCGA 1 cut(s) 62
BspACI CCGC 5 cut(s) 47, 75, 91, 208, 234
BspANI GGCC 2 cut(s) 25, 50
BspCNI CTCAG 1 cut(s) 12
BspFNI CGCG 2 cut(s) 47, 62
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 217
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 1 cut(s) 69
Bst4CI ACNGT 2 cut(s) 42, 245
BstC8I GCNNGC 2 cut(s) 17, 234
BstDEI CTNAG 1 cut(s) 20
BstFNI CGCG 2 cut(s) 47, 62
BstKTI GATC 1 cut(s) 72
BstMBI GATC 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstSLI GKGCMC 1 cut(s) 220
BstUI CGCG 2 cut(s) 47, 62
BstV1I GCAGC 1 cut(s) 127
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
BsuRI GGCC 2 cut(s) 25, 50
BtsIMutI CAGTG 1 cut(s) 38
BtuMI TCGCGA 1 cut(s) 62
Cac8I GCNNGC 2 cut(s) 17, 234
Cfr13I GGNCC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 178
CviJI RGCY 5 cut(s) 5, 19, 25, 50, 140
CviKI_1 RGCY 5 cut(s) 5, 19, 25, 50, 140
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 1 cut(s) 20
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 48
EciI GGCGGA 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 26
FaiI YATR 1 cut(s) 230
FauI CCCGC 2 cut(s) 68, 241
Fnu4HI GCNGC 3 cut(s) 6, 48, 141
Fsp4HI GCNGC 3 cut(s) 6, 48, 141
GluI GCNGC 3 cut(s) 6, 48, 141
GsuI CTGGAG 1 cut(s) 165
HaeIII GGCC 2 cut(s) 25, 50
HapII CCGG 1 cut(s) 221
HinfI GANTC 2 cut(s) 10, 82
HpaII CCGG 1 cut(s) 221
HphI GGTGA 3 cut(s) 91, 133, 244
Hpy188III TCNNGA 2 cut(s) 61, 193
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 2 cut(s) 42, 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 1 cut(s) 20
Kzo9I GATC 1 cut(s) 69
LpnPI CCDG 6 cut(s) 39, 103, 123, 129, 199, 234
Lsp1109I GCAGC 1 cut(s) 127
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 1 cut(s) 241
MflI RGATCY 1 cut(s) 69
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 4 cut(s) 187, 200, 225, 268
MlyI GAGTC 1 cut(s) 91
MnlI CCTC 4 cut(s) 15, 22, 124, 189
MspI CCGG 1 cut(s) 221
MvnI CGCG 2 cut(s) 47, 62
MwoI GCNNNNNNNGC 1 cut(s) 132
NdeII GATC 1 cut(s) 69
NlaIV GGNNCC 1 cut(s) 219
NruI TCGCGA 1 cut(s) 62
PfeI GAWTC 1 cut(s) 10
PkrI GCNGC 3 cut(s) 7, 49, 142
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 24
PsuI RGATCY 1 cut(s) 69
RruI TCGCGA 1 cut(s) 62
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SatI GCNGC 3 cut(s) 6, 48, 141
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 1 cut(s) 24
SchI GAGTC 1 cut(s) 91
SduI GDGCHC 1 cut(s) 220
SetI ASST 2 cut(s) 33, 142
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 4 cut(s) 187, 200, 225, 268
SsiI CCGC 5 cut(s) 47, 75, 91, 208, 234
TaaI ACNGT 2 cut(s) 42, 245
TasI AATT 4 cut(s) 187, 200, 225, 268
TatI WGTACW 1 cut(s) 177
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 1 cut(s) 10
TscAI CASTG 1 cut(s) 45
TseI GCWGC 2 cut(s) 5, 140
TspRI CASTG 1 cut(s) 45
XapI RAATTY 2 cut(s) 187, 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.