pycom15g00090

Zn_pept

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008016
Physical Location & Seq
Forward (+)
60275 .. 63555
3281 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1314 bp
ATGGCTCATCTCCTTCCTCTCTCCTCTCCTTTATCCATCACTCTCTGCTTCTTTGCGTGGTTACAAGCTTTCGTGGTCGTCCATGGCAATAGTAATCTCACCCACCCTTCTCTCACCCCTATCAACCGCGATCTCTATCACTCCAGTGGGGATTTGATGGAAGAAATTAAAGCTCTCGTCTTTCGGCACCCAGAGGAGCTCTCTTTGGATACAATTAAATCTCAAAACAAGGGCTACTCTGCTGAGATCGCAATAGTTACCTATAGTCGGAGAAGTCAAGAGACTGATGACCGGCCAAAATTTCGGATCCTTCTTAGTTTTGGGCAGCATGGAAGGGAGCTTATTACATCCGAGCTTGCATTGCGAATCCTATCAATCTGTAGTAAAGAACAGTTTCTACCCAACCTGGACCCAGCCTCCTTAGACAGTACCCTGGACAAGCTCTTGATAAAGGTGGTTCCAATAGAAAACTTAAACGGCCGTAAACTTGTTGAAGCAGGAGACCTCTGTGAGAGAAGAAATGGGAGGGGAGTTGATCTCAACCGTAATTGGAGCGTAGATTGGGGCAAAAAAGAGAAGGATTATGATCGATATGAGGAATCTCCTGGAACTGCTCCTTTCAGCGAGCCTGAAACTCAGATAATACGGAAAGTTGCCGTGTCATTTGATCCACACATCTGGATTAATATACACTCTGGGATGGAGGCTCTGTTTATGCCGTATGACCATAAGAACACAACCCCAGATGGAGTTTCCTCCCACCAGATGAAGTCATTGCTTGAAGAACTCAACCAACTGCACTGCCAAAAGCGTTGCATGGTTGGGTCTGGTGGAGGTTCTGTTGGGTACCTGGCACATGGGACAGCAACTGATTTTATGTTCGACATTGTAAGGGTGCCAATGGCTTTCACCTTTGAGATATATGGAGATGGAGCGGCCTCATCAAGAGATTGCTTCAAAATGTTCAATCCCACCGACTTCGTTACCTTCAATAGAGTTCTCAATGAATGGTCTGCTGCATTTTTCACAATTTTTAAATTGGGGCCAGACCAGCTTGGTGAAAATTATTCAAAGGCTTCTGTGCCCACCTTATTAGACAAGTGGGTATCCATAGATGAGTATCTTGAAGGTTATTTAGTCGAGAGGAGTAGTAGATATGGAAAGAAGATGGAGGTGCTTGAACTAGGAATGCAGGAGATAAGAACATATTTCAGGCTCTTCTTGTTATCTTCTGTTCTGTTAATGTTCATGTTCTGTTCCAGAATTTCAAAAAGCAAGTGTGCTAGACCAATGGTTTCTGCTATTGCAATCTGA

Protein Analysis

438

Amino Acids

49.54

Weight (kDa)

6.2

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M14 PF00246 55 - 320 6.7e-25 Zinc carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 846
AccB1I GGYRCC 3 cut(s) 186, 846, 895
AccBSI CCGCTC 1 cut(s) 935
AccII CGCG 1 cut(s) 129
AciI CCGC 2 cut(s) 127, 935
AclWI GGATC 3 cut(s) 301, 314, 662
AcoI YGGCCR 2 cut(s) 293, 478
AcsI RAATTY 2 cut(s) 299, 1263
AfaI GTAC 2 cut(s) 430, 848
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 9 cut(s) 494, 782, 958, 967, 991, 1071, 1127, 1181, 1269
AjnI CCWGG 4 cut(s) 405, 432, 604, 849
AleI CACNNNNGTG 1 cut(s) 144
AluBI AGCT 7 cut(s) 68, 173, 199, 340, 355, 442, 1054
AluI AGCT 7 cut(s) 68, 173, 199, 340, 355, 442, 1054
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 2 cut(s) 275, 495
AlwI GGATC 3 cut(s) 301, 314, 662
AlwNI CAGNNNCTG 1 cut(s) 869
AoxI GGCC 4 cut(s) 293, 478, 936, 1043
ApeKI GCWGC 2 cut(s) 325, 1016
ApoI RAATTY 2 cut(s) 299, 1263
AseI ATTAAT 1 cut(s) 684
Asp700I GAANNNNTTC 1 cut(s) 393
Asp718I GGTACC 1 cut(s) 846
AspS9I GGNCC 2 cut(s) 409, 1043
AsuHPI GGTGA 4 cut(s) 91, 106, 901, 1070
AvaII GGWCC 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 1086
BamHI GGATCC 1 cut(s) 306
BanI GGYRCC 3 cut(s) 186, 846, 895
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 2 cut(s) 337, 1003
BccI CCATC 6 cut(s) 44, 151, 694, 740, 923, 1162
BceAI ACGGC 4 cut(s) 465, 493, 641, 703
BciT130I CCWGG 4 cut(s) 407, 434, 606, 851
BciVI GTATCC 2 cut(s) 202, 1117
BcoDI GTCTC 2 cut(s) 275, 495
BfaI CTAG 2 cut(s) 1184, 1284
BfmI CTRYAG 2 cut(s) 262, 379
BfuI GTATCC 2 cut(s) 202, 1117
BisI GCNGC 3 cut(s) 326, 936, 1017
BlsI GCNGC 3 cut(s) 327, 937, 1018
Bme1390I CCNGG 4 cut(s) 407, 434, 606, 851
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 2 cut(s) 409, 1043
BmiI GGNNCC 7 cut(s) 188, 308, 411, 459, 848, 897, 1044
BmrFI CCNGG 4 cut(s) 407, 434, 606, 851
BplI GAGNNNNNCTC 4 cut(s) 185, 217, 522, 554
BpmI CTGGAG 1 cut(s) 127
Bsa29I ATCGAT 1 cut(s) 589
BsaBI GATNNNNATC 3 cut(s) 135, 585, 1119
BsaI GGTCTC 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 82, 432
BsaXI ACNNNNNCTCC 6 cut(s) 401, 431, 695, 725, 825, 855
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse118I RCCGGY 1 cut(s) 291
Bse1I ACTGG 1 cut(s) 144
Bse3DI GCAATG 2 cut(s) 359, 773
Bse8I GATNNNNATC 3 cut(s) 135, 585, 1119
BseBI CCWGG 4 cut(s) 407, 434, 606, 851
BseCI ATCGAT 1 cut(s) 589
BseDI CCNNGG 2 cut(s) 82, 432
BseGI GGATG 2 cut(s) 347, 705
BseJI GATNNNNATC 3 cut(s) 135, 585, 1119
BseLI CCNNNNNNNGG 1 cut(s) 267
BseMI GCAATG 2 cut(s) 359, 773
BseMII CTCAG 2 cut(s) 234, 650
BseNI ACTGG 1 cut(s) 144
BseRI GAGGAG 3 cut(s) 13, 209, 1159
BseSI GKGCMC 1 cut(s) 1086
BseX3I CGGCCG 1 cut(s) 478
BseXI GCAGC 2 cut(s) 337, 1003
BseYI CCCAGC 1 cut(s) 412
BsgI GTGCAG 1 cut(s) 782
Bsh1236I CGCG 1 cut(s) 129
Bsh1285I CGRYCG 1 cut(s) 481
BshFI GGCC 4 cut(s) 295, 480, 938, 1045
BshNI GGYRCC 3 cut(s) 186, 846, 895
BshVI ATCGAT 1 cut(s) 589
BsiEI CGRYCG 1 cut(s) 481
BsiHKAI GWGCWC 1 cut(s) 201
BsiSI CCGG 1 cut(s) 292
BslFI GGGAC 1 cut(s) 874
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 2 cut(s) 275, 495
BsmFI GGGAC 1 cut(s) 874
BsmI GAATGC 1 cut(s) 1194
BsnI GGCC 4 cut(s) 295, 480, 938, 1045
Bso31I GGTCTC 1 cut(s) 495
Bsp1286I GDGCHC 2 cut(s) 201, 1086
Bsp143I GATC 6 cut(s) 130, 246, 306, 535, 586, 667
Bsp19I CCATGG 1 cut(s) 82
BspACI CCGC 2 cut(s) 127, 935
BspANI GGCC 4 cut(s) 295, 480, 938, 1045
BspCNI CTCAG 2 cut(s) 235, 649
BspDI ATCGAT 1 cut(s) 589
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 7 cut(s) 188, 308, 411, 459, 848, 897, 1044
BspPI GGATC 3 cut(s) 301, 314, 662
BspQI GCTCTTC 1 cut(s) 1223
BspT107I GGYRCC 3 cut(s) 186, 846, 895
BspTNI GGTCTC 1 cut(s) 495
BsrBI CCGCTC 1 cut(s) 935
BsrDI GCAATG 2 cut(s) 359, 773
BsrFI RCCGGY 1 cut(s) 291
BsrI ACTGG 1 cut(s) 144
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 2 cut(s) 82, 432
BssMI GATC 6 cut(s) 130, 246, 306, 535, 586, 667
BssT1I CCWWGG 1 cut(s) 82
Bst2UI CCWGG 4 cut(s) 407, 434, 606, 851
Bst4CI ACNGT 3 cut(s) 393, 428, 545
Bst6I CTCTTC 1 cut(s) 1223
BstC8I GCNNGC 2 cut(s) 357, 626
BstDEI CTNAG 4 cut(s) 243, 314, 421, 636
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 2 cut(s) 347, 705
BstFNI CGCG 1 cut(s) 129
BstKTI GATC 6 cut(s) 133, 249, 309, 538, 589, 670
BstMAI GTCTC 2 cut(s) 275, 495
BstMBI GATC 6 cut(s) 130, 246, 306, 535, 586, 667
BstMCI CGRYCG 1 cut(s) 481
BstMWI GCNNNNNNNGC 3 cut(s) 248, 361, 1051
BstNI CCWGG 4 cut(s) 407, 434, 606, 851
BstSCI CCNGG 4 cut(s) 405, 432, 604, 849
BstSFI CTRYAG 2 cut(s) 262, 379
BstSLI GKGCMC 1 cut(s) 1086
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 337, 1003
BstX2I RGATCY 1 cut(s) 306
BstXI CCANNNNNNTGG 1 cut(s) 678
BstYI RGATCY 1 cut(s) 306
BstZI CGGCCG 1 cut(s) 478
Bsu15I ATCGAT 1 cut(s) 589
BsuI GTATCC 2 cut(s) 202, 1117
BsuRI GGCC 4 cut(s) 295, 480, 938, 1045
BsuTUI ATCGAT 1 cut(s) 589
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 2 cut(s) 347, 705
BtsI GCAGTG 1 cut(s) 799
BtsIMutI CAGTG 2 cut(s) 151, 799
Cac8I GCNNGC 2 cut(s) 357, 626
CaiI CAGNNNCTG 1 cut(s) 869
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 2 cut(s) 409, 1043
ClaI ATCGAT 1 cut(s) 589
Csp6I GTAC 2 cut(s) 429, 847
CviAII CATG 5 cut(s) 83, 329, 817, 857, 1249
CviQI GTAC 2 cut(s) 429, 847
DdeI CTNAG 4 cut(s) 243, 314, 421, 636
DpnI GATC 6 cut(s) 132, 248, 308, 537, 588, 669
DpnII GATC 6 cut(s) 130, 246, 306, 535, 586, 667
DraI TTTAAA 1 cut(s) 1036
EaeI YGGCCR 2 cut(s) 293, 478
EagI CGGCCG 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 1223
EarI CTCTTC 1 cut(s) 1223
Ecl136II GAGCTC 1 cut(s) 199
EclXI CGGCCG 1 cut(s) 478
Eco130I CCWWGG 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 409
Eco52I CGGCCG 1 cut(s) 478
Eco53kI GAGCTC 1 cut(s) 199
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 4 cut(s) 405, 432, 604, 849
EcoT14I CCWWGG 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 5 cut(s) 86, 332, 820, 860, 1252
FaqI GGGAC 1 cut(s) 874
FatI CATG 5 cut(s) 82, 328, 816, 856, 1248
Fnu4HI GCNGC 3 cut(s) 326, 936, 1017
FokI GGATG 2 cut(s) 334, 712
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 3 cut(s) 326, 936, 1017
FspBI CTAG 2 cut(s) 1184, 1284
GluI GCNGC 3 cut(s) 326, 936, 1017
GsaI CCCAGC 1 cut(s) 416
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 4 cut(s) 295, 480, 938, 1045
HapII CCGG 1 cut(s) 292
Hin1II CATG 5 cut(s) 86, 332, 820, 860, 1252
HindIII AAGCTT 1 cut(s) 66
HinfI GANTC 2 cut(s) 366, 599
HpaII CCGG 1 cut(s) 292
HphI GGTGA 4 cut(s) 91, 106, 901, 1070
Hpy166II GTNNAC 1 cut(s) 485
Hpy188I TCNGA 5 cut(s) 270, 306, 352, 639, 1313
Hpy188III TCNNGA 7 cut(s) 278, 445, 679, 945, 1124, 1141, 1260
Hpy8I GTNNAC 1 cut(s) 485
HpyAV CCTTC 7 cut(s) 23, 117, 320, 327, 571, 997, 1121
HpyCH4III ACNGT 3 cut(s) 393, 428, 545
HpyCH4V TGCA 6 cut(s) 359, 799, 816, 1019, 1192, 1307
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 361, 1051
HpyF3I CTNAG 4 cut(s) 243, 314, 421, 636
Hsp92II CATG 5 cut(s) 86, 332, 820, 860, 1252
KpnI GGTACC 1 cut(s) 850
Kzo9I GATC 6 cut(s) 130, 246, 306, 535, 586, 667
LguI GCTCTTC 1 cut(s) 1223
LmnI GCTCC 5 cut(s) 196, 337, 552, 619, 932
Lsp1109I GCAGC 2 cut(s) 337, 1003
MaeI CTAG 2 cut(s) 1184, 1284
MaeIII GTNAC 3 cut(s) 60, 256, 982
MalI GATC 6 cut(s) 132, 248, 308, 537, 588, 669
MbiI CCGCTC 1 cut(s) 935
MboI GATC 6 cut(s) 130, 246, 306, 535, 586, 667
MboII GAAGA 6 cut(s) 173, 528, 794, 1177, 1210, 1221
MflI RGATCY 1 cut(s) 306
MhlI GDGCHC 2 cut(s) 201, 1086
MluCI AATT 8 cut(s) 165, 213, 299, 547, 1029, 1037, 1063, 1263
MmeI TCCRAC 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 393
MseI TTAA 6 cut(s) 168, 216, 473, 684, 1035, 1241
MslI CAYNNNNRTG 1 cut(s) 144
MspI CCGG 1 cut(s) 292
MspR9I CCNGG 4 cut(s) 407, 434, 606, 851
Mva1269I GAATGC 1 cut(s) 1194
MvaI CCWGG 4 cut(s) 407, 434, 606, 851
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 248, 361, 1051
NcoI CCATGG 1 cut(s) 82
NdeII GATC 6 cut(s) 130, 246, 306, 535, 586, 667
NlaIII CATG 5 cut(s) 86, 332, 820, 860, 1252
NlaIV GGNNCC 7 cut(s) 188, 308, 411, 459, 848, 897, 1044
OliI CACNNNNGTG 1 cut(s) 144
PciSI GCTCTTC 1 cut(s) 1223
PctI GAATGC 1 cut(s) 1194
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 2 cut(s) 366, 599
PfoI TCCNGGA 1 cut(s) 604
PkrI GCNGC 3 cut(s) 327, 937, 1018
PshBI ATTAAT 1 cut(s) 684
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 4 cut(s) 405, 432, 604, 849
PspFI CCCAGC 1 cut(s) 412
PspGI CCWGG 4 cut(s) 405, 432, 604, 849
PspN4I GGNNCC 7 cut(s) 188, 308, 411, 459, 848, 897, 1044
PspPI GGNCC 2 cut(s) 409, 1043
PsrI GAACNNNNNNTAC 2 cut(s) 381, 413
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 1 cut(s) 306
RsaI GTAC 2 cut(s) 430, 848
RsaNI GTAC 2 cut(s) 429, 847
RseI CAYNNNNRTG 1 cut(s) 144
SacI GAGCTC 1 cut(s) 201
SapI GCTCTTC 1 cut(s) 1223
SaqAI TTAA 6 cut(s) 168, 216, 473, 684, 1035, 1241
SatI GCNGC 3 cut(s) 326, 936, 1017
Sau3AI GATC 6 cut(s) 130, 246, 306, 535, 586, 667
Sau96I GGNCC 2 cut(s) 409, 1043
ScrFI CCNGG 4 cut(s) 407, 434, 606, 851
SduI GDGCHC 2 cut(s) 201, 1086
SfcI CTRYAG 2 cut(s) 262, 379
SinI GGWCC 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 144
Sse9I AATT 8 cut(s) 165, 213, 299, 547, 1029, 1037, 1063, 1263
SsiI CCGC 2 cut(s) 127, 935
SspMI CTAG 2 cut(s) 1184, 1284
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 4 cut(s) 405, 432, 604, 849
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 3 cut(s) 393, 428, 545
TaqI TCGA 3 cut(s) 589, 882, 1140
TasI AATT 8 cut(s) 165, 213, 299, 547, 1029, 1037, 1063, 1263
TauI GCSGC 1 cut(s) 938
TfiI GAWTC 2 cut(s) 366, 599
Tru1I TTAA 6 cut(s) 168, 216, 473, 684, 1035, 1241
Tru9I TTAA 6 cut(s) 168, 216, 473, 684, 1035, 1241
TscAI CASTG 2 cut(s) 151, 806
TseI GCWGC 2 cut(s) 325, 1016
TspDTI ATGAA 3 cut(s) 782, 1020, 1237
TspGWI ACGGA 1 cut(s) 661
TspRI CASTG 2 cut(s) 151, 806
VpaK11BI GGWCC 1 cut(s) 409
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 2 cut(s) 299, 1263
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 2 cut(s) 1184, 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.