pycom15g02370

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010596
Physical Location & Seq
Forward (+)
1386868 .. 1388025
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
ATGAAGTCAGTGACGTGGGTGTCAAAATATACCAGAAGCCAGACTCAAATGATATATATCGATCTGAGGAGGAAGAAATATCCACCTTTGTGCAAGGAAAATGAAAACCCAGATGCAGCCTGGTATGTTCCCGTGAAGACTTGCTTGCACCCCATTCCATCTGCCATTGAACAGCGTGGCACAGAGTGGCCAGAGGAATGGCCCAAAAGGCTCGAAAGGTATCCTGACTGGTTAAGTGATAAAGATAAATTGGATGCAGACACCAAGCACTGGAAAGCTATTGTTGATAAATCTTATCTCACTGGACTGGGTATTGACTGGTCAAAAATTCGAAATGTAATGGACATGAAAGCCATCTATGGAGGATTTGCTGCAGCCCTCGCACAACAAAAGGTTTGGGTGATGAATGTGGTACCTGTCCATGCTCCTGATACACTTCCTTTCATATTTGAACGCGGGCTTGTTGGAACCTACCACGACTGGTGCGAGTCTTTCGGTACTTATCCCAGATCATATGACCTTTTGCATGTGGATCATCTGTTTTCACGGCTTAAGAATAGGTGTAAGCAGCCTGTATCAATCGTTGTTGAGATGGATCGCTTGTTACGGCCTGGAGGTTGGGCGATTATACGCGAGAAAGTTGAGGTACTGGAACCCCTGGAGGGTATATTGAAGAGTATGCATTGGGAGATCCGGATGACTTATGCTCAGGGAAAGGAGGGTATCTTATGCGCCCAGAAAACGTTGTGGCGGCCTTAA

Protein Analysis

253

Amino Acids

29.5

Weight (kDa)

8.95

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 10 - 247 5.6e-92 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 19
Acc65I GGTACC 1 cut(s) 412
AccB1I GGYRCC 1 cut(s) 412
AccB7I CCANNNNNTGG 1 cut(s) 270
AccII CGCG 2 cut(s) 456, 633
AccIII TCCGGA 1 cut(s) 693
AciI CCGC 2 cut(s) 456, 751
AclI AACGTT 1 cut(s) 743
AclWI GGATC 3 cut(s) 540, 603, 685
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 1 cut(s) 327
AdeI CACNNNGTG 1 cut(s) 186
AfaI GTAC 3 cut(s) 414, 499, 648
AfiI CCNNNNNNNGG 2 cut(s) 270, 662
AflII CTTAAG 1 cut(s) 551
AgsI TTSAA 3 cut(s) 170, 452, 673
AjiI CACGTC 1 cut(s) 15
AjnI CCWGG 3 cut(s) 119, 610, 657
AleI CACNNNNGTG 1 cut(s) 88
AluBI AGCT 1 cut(s) 278
AluI AGCT 1 cut(s) 278
AlwI GGATC 3 cut(s) 540, 603, 685
Aor13HI TCCGGA 1 cut(s) 693
AoxI GGCC 4 cut(s) 188, 200, 608, 752
ApeKI GCWGC 4 cut(s) 116, 371, 374, 568
ApoI RAATTY 1 cut(s) 327
Asp718I GGTACC 1 cut(s) 412
AspLEI GCGC 1 cut(s) 734
AspS9I GGNCC 1 cut(s) 201
AsuHPI GGTGA 1 cut(s) 412
AsuII TTCGAA 1 cut(s) 331
BalI TGGCCA 1 cut(s) 190
BanI GGYRCC 1 cut(s) 412
BbsI GAAGAC 1 cut(s) 143
BbvI GCAGC 4 cut(s) 128, 358, 386, 580
BccI CCATC 3 cut(s) 166, 362, 586
BceAI ACGGC 2 cut(s) 563, 623
BciT130I CCWGG 3 cut(s) 121, 612, 659
BciVI GTATCC 1 cut(s) 231
BfmI CTRYAG 1 cut(s) 372
BfrI CTTAAG 1 cut(s) 551
BfuI GTATCC 1 cut(s) 231
BglI GCCNNNNNGGC 1 cut(s) 208
BisI GCNGC 5 cut(s) 117, 372, 375, 569, 752
BlsI GCNGC 5 cut(s) 118, 373, 376, 570, 753
Bme1390I CCNGG 3 cut(s) 121, 612, 659
BmgBI CACGTC 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 201
BmiI GGNNCC 3 cut(s) 414, 469, 654
BmrFI CCNGG 3 cut(s) 121, 612, 659
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 2 cut(s) 103, 244
BmuI ACTGGG 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 143
BpmI CTGGAG 2 cut(s) 633, 680
Bpu10I CCTNAGC 1 cut(s) 708
Bpu14I TTCGAA 1 cut(s) 331
Bsa29I ATCGAT 1 cut(s) 60
BsaBI GATNNNNATC 1 cut(s) 56
BsaJI CCNNGG 1 cut(s) 657
BsaWI WCCGGW 1 cut(s) 693
Bsc4I CCNNNNNNNGG 2 cut(s) 270, 662
Bse1I ACTGG 7 cut(s) 233, 275, 307, 312, 323, 485, 654
Bse8I GATNNNNATC 1 cut(s) 56
BseAI TCCGGA 1 cut(s) 693
BseBI CCWGG 3 cut(s) 121, 612, 659
BseCI ATCGAT 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 657
BseGI GGATG 2 cut(s) 259, 702
BseJI GATNNNNATC 1 cut(s) 56
BseLI CCNNNNNNNGG 2 cut(s) 270, 662
BseMII CTCAG 2 cut(s) 56, 722
BseNI ACTGG 7 cut(s) 233, 275, 307, 312, 323, 485, 654
BseRI GAGGAG 1 cut(s) 82
BseXI GCAGC 4 cut(s) 128, 358, 386, 580
Bsh1236I CGCG 2 cut(s) 456, 633
BshFI GGCC 4 cut(s) 190, 202, 610, 754
BshNI GGYRCC 1 cut(s) 412
BshVI ATCGAT 1 cut(s) 60
BsiSI CCGG 1 cut(s) 694
BslI CCNNNNNNNGG 2 cut(s) 270, 662
BsnI GGCC 4 cut(s) 190, 202, 610, 754
Bsp119I TTCGAA 1 cut(s) 331
Bsp13I TCCGGA 1 cut(s) 693
Bsp143I GATC 5 cut(s) 61, 509, 532, 595, 690
BspACI CCGC 2 cut(s) 456, 751
BspANI GGCC 4 cut(s) 190, 202, 610, 754
BspCNI CTCAG 2 cut(s) 57, 721
BspDI ATCGAT 1 cut(s) 60
BspEI TCCGGA 1 cut(s) 693
BspFNI CGCG 2 cut(s) 456, 633
BspLI GGNNCC 3 cut(s) 414, 469, 654
BspMAI CTGCAG 1 cut(s) 376
BspPI GGATC 3 cut(s) 540, 603, 685
BspT104I TTCGAA 1 cut(s) 331
BspT107I GGYRCC 1 cut(s) 412
BspTI CTTAAG 1 cut(s) 551
BsrI ACTGG 7 cut(s) 233, 275, 307, 312, 323, 485, 654
BssECI CCNNGG 1 cut(s) 657
BssMI GATC 5 cut(s) 61, 509, 532, 595, 690
Bst2UI CCWGG 3 cut(s) 121, 612, 659
Bst6I CTCTTC 1 cut(s) 668
BstAFI CTTAAG 1 cut(s) 551
BstBI TTCGAA 1 cut(s) 331
BstC8I GCNNGC 2 cut(s) 146, 458
BstDEI CTNAG 2 cut(s) 65, 708
BstF5I GGATG 2 cut(s) 259, 702
BstFNI CGCG 2 cut(s) 456, 633
BstHHI GCGC 1 cut(s) 734
BstKTI GATC 5 cut(s) 64, 512, 535, 598, 693
BstMBI GATC 5 cut(s) 61, 509, 532, 595, 690
BstMWI GCNNNNNNNGC 2 cut(s) 208, 380
BstNI CCWGG 3 cut(s) 121, 612, 659
BstNSI RCATGY 1 cut(s) 530
BstSCI CCNGG 3 cut(s) 119, 610, 657
BstSFI CTRYAG 1 cut(s) 372
BstUI CGCG 2 cut(s) 456, 633
BstV1I GCAGC 4 cut(s) 128, 358, 386, 580
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 1 cut(s) 690
BstXI CCANNNNNNTGG 1 cut(s) 198
BstYI RGATCY 1 cut(s) 690
Bsu15I ATCGAT 1 cut(s) 60
BsuI GTATCC 1 cut(s) 231
BsuRI GGCC 4 cut(s) 190, 202, 610, 754
BsuTUI ATCGAT 1 cut(s) 60
BtrI CACGTC 1 cut(s) 15
BtsCI GGATG 2 cut(s) 259, 702
BtsIMutI CAGTG 3 cut(s) 15, 268, 300
Cac8I GCNNGC 2 cut(s) 146, 458
CfoI GCGC 1 cut(s) 734
Cfr13I GGNCC 1 cut(s) 201
ClaI ATCGAT 1 cut(s) 60
Csp6I GTAC 3 cut(s) 413, 498, 647
CviAII CATG 3 cut(s) 346, 422, 527
CviQI GTAC 3 cut(s) 413, 498, 647
DdeI CTNAG 2 cut(s) 65, 708
DpnI GATC 5 cut(s) 63, 511, 534, 597, 692
DpnII GATC 5 cut(s) 61, 509, 532, 595, 690
DraIII CACNNNGTG 1 cut(s) 186
DrdI GACNNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 19
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 668
EarI CTCTTC 1 cut(s) 668
EcoRII CCWGG 3 cut(s) 119, 610, 657
EcoT22I ATGCAT 1 cut(s) 684
FaeI CATG 3 cut(s) 349, 425, 530
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FatI CATG 3 cut(s) 345, 421, 526
FauI CCCGC 1 cut(s) 449
FauNDI CATATG 1 cut(s) 514
Fnu4HI GCNGC 5 cut(s) 117, 372, 375, 569, 752
FokI GGATG 2 cut(s) 266, 709
Fsp4HI GCNGC 5 cut(s) 117, 372, 375, 569, 752
GlaI GCGC 1 cut(s) 733
GluI GCNGC 5 cut(s) 117, 372, 375, 569, 752
GsuI CTGGAG 2 cut(s) 633, 680
HaeIII GGCC 4 cut(s) 190, 202, 610, 754
HapII CCGG 1 cut(s) 694
HhaI GCGC 1 cut(s) 734
Hin1II CATG 3 cut(s) 349, 425, 530
Hin6I GCGC 1 cut(s) 732
HinP1I GCGC 1 cut(s) 732
HinfI GANTC 2 cut(s) 43, 488
HpaII CCGG 1 cut(s) 694
HphI GGTGA 1 cut(s) 412
Hpy188I TCNGA 1 cut(s) 66
Hpy188III TCNNGA 3 cut(s) 224, 428, 694
HpyCH4IV ACGT 2 cut(s) 14, 743
HpyCH4V TGCA 7 cut(s) 93, 116, 148, 257, 374, 526, 682
HpyF10VI GCNNNNNNNGC 2 cut(s) 208, 380
HpyF3I CTNAG 2 cut(s) 65, 708
HpySE526I ACGT 2 cut(s) 14, 743
Hsp92II CATG 3 cut(s) 349, 425, 530
HspAI GCGC 1 cut(s) 732
Kpn2I TCCGGA 1 cut(s) 693
KpnI GGTACC 1 cut(s) 416
Kzo9I GATC 5 cut(s) 61, 509, 532, 595, 690
LmnI GCTCC 1 cut(s) 430
Lsp1109I GCAGC 4 cut(s) 128, 358, 386, 580
LweI GCATC 2 cut(s) 103, 244
MaeII ACGT 2 cut(s) 14, 743
MaeIII GTNAC 2 cut(s) 10, 603
MalI GATC 5 cut(s) 63, 511, 534, 597, 692
MboI GATC 5 cut(s) 61, 509, 532, 595, 690
MboII GAAGA 3 cut(s) 85, 148, 685
MflI RGATCY 1 cut(s) 690
MlsI TGGCCA 1 cut(s) 190
MluCI AATT 2 cut(s) 248, 327
MluNI TGGCCA 1 cut(s) 190
MlyI GAGTC 2 cut(s) 37, 497
MmeI TCCRAC 1 cut(s) 445
MnlI CCTC 9 cut(s) 60, 63, 187, 356, 389, 608, 637, 655, 712
Mox20I TGGCCA 1 cut(s) 190
Mph1103I ATGCAT 1 cut(s) 684
MroI TCCGGA 1 cut(s) 693
MscI TGGCCA 1 cut(s) 190
MseI TTAA 3 cut(s) 233, 552, 757
MslI CAYNNNNRTG 1 cut(s) 88
Msp20I TGGCCA 1 cut(s) 190
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 1 cut(s) 694
MspR9I CCNGG 3 cut(s) 121, 612, 659
MvaI CCWGG 3 cut(s) 121, 612, 659
MvnI CGCG 2 cut(s) 456, 633
MwoI GCNNNNNNNGC 2 cut(s) 208, 380
NdeI CATATG 1 cut(s) 514
NdeII GATC 5 cut(s) 61, 509, 532, 595, 690
NlaIII CATG 3 cut(s) 349, 425, 530
NlaIV GGNNCC 3 cut(s) 414, 469, 654
NmuCI GTSAC 1 cut(s) 10
NsiI ATGCAT 1 cut(s) 684
NspI RCATGY 1 cut(s) 530
NspV TTCGAA 1 cut(s) 331
OliI CACNNNNGTG 1 cut(s) 88
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PflMI CCANNNNNTGG 1 cut(s) 270
PkrI GCNGC 5 cut(s) 118, 373, 376, 570, 753
PleI GAGTC 2 cut(s) 37, 496
PpsI GAGTC 2 cut(s) 37, 496
Psp1406I AACGTT 1 cut(s) 743
Psp6I CCWGG 3 cut(s) 119, 610, 657
PspGI CCWGG 3 cut(s) 119, 610, 657
PspN4I GGNNCC 3 cut(s) 414, 469, 654
PspPI GGNCC 1 cut(s) 201
PstI CTGCAG 1 cut(s) 376
PsuI RGATCY 1 cut(s) 690
RsaI GTAC 3 cut(s) 414, 499, 648
RsaNI GTAC 3 cut(s) 413, 498, 647
RseI CAYNNNNRTG 1 cut(s) 88
SaqAI TTAA 3 cut(s) 233, 552, 757
SatI GCNGC 5 cut(s) 117, 372, 375, 569, 752
Sau3AI GATC 5 cut(s) 61, 509, 532, 595, 690
Sau96I GGNCC 1 cut(s) 201
SchI GAGTC 2 cut(s) 37, 497
ScrFI CCNGG 3 cut(s) 121, 612, 659
SfaNI GCATC 2 cut(s) 103, 244
SfcI CTRYAG 1 cut(s) 372
SfuI TTCGAA 1 cut(s) 331
SmiMI CAYNNNNRTG 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 2 cut(s) 248, 327
SsiI CCGC 2 cut(s) 456, 751
StyD4I CCNGG 3 cut(s) 119, 610, 657
TaiI ACGT 2 cut(s) 17, 746
TaqI TCGA 3 cut(s) 60, 213, 331
TasI AATT 2 cut(s) 248, 327
TauI GCSGC 1 cut(s) 754
Tru1I TTAA 3 cut(s) 233, 552, 757
Tru9I TTAA 3 cut(s) 233, 552, 757
TscAI CASTG 3 cut(s) 15, 275, 307
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 4 cut(s) 116, 371, 374, 568
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 5 cut(s) 17, 117, 362, 419, 433
TspRI CASTG 3 cut(s) 15, 275, 307
Van91I CCANNNNNTGG 1 cut(s) 270
Vha464I CTTAAG 1 cut(s) 551
XapI RAATTY 1 cut(s) 327
XceI RCATGY 1 cut(s) 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 117
Zsp2I ATGCAT 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.