pycom15g05020

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010419
Physical Location & Seq
Reverse (-)
3014813 .. 3017625
2813 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 924 bp
ATGGCGTCGGCTTACAAGCCCGAAGAAGCCAGGGTCCCGCCTGCTCTTCCCCTACCCACTCCTCCCGTCACCAAGTTTAAGATTGCGCTGTGCCAATTGTCGGTGACGCCGGATAAGGAGAGGAACATTTCCCACGCTCAGAAAGCCATCGAGGACGCCGCCGCAAAGGGTGCCCAACTTGTTGTCCTCCCTGAAATTTGGAATGGTCCATATTCAAATGACAGCTTTCCGGTTTATGCCGAGGACATTGATGCGGGTGGCGATGCATCTCCTTCAACAGCCATGCTGTCTGAAGTTGCACAACGTCTGAAGATCACCATTGTTGGTGGATCTATAGCGGAGCGTTCTGGGGATCGGTTGTACAATACTTCCTGTGTCTTTGACACTGACGGAAAGCTGTTAGCTAAACACAGGAAGATACACCTATTTGATATCGACATCCCGGGGAAGATTACCTTCATTGAATCAAAGACTCTCACAGCAGGGCAGACTCCTACCATTGTGGATACAGAAGTTGGGCGTATTGGCATAGGTATCTGTTATGACATTAGGTTTCAGGAACTAGCAATGATTTATGCAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACCACTGGACCGTTGCACTGGGAATTATTGCAGAGGGCAAGGGCTACAGATAACCAGCTATACGTAGCAACTTGTTCCCCTGCTCGAGATGCTGGAGCCGGTTATGTGGCATGGGGCCACTCCACCCTTGTTGGACCATTTGGAGAAGTACTGGCGACGACGGAACATGAAGAGGCAATAATCATAGCAGAGATTGATTATTCAATTATTGAGCTTAGAAGGACAAACCTCCCCTTACTGAAGCAAAGACGAGGCGATCTTTACCAGTTGGTAGATGTTGAGAGGTTAAATTCTCAATGA

Protein Analysis

308

Amino Acids

33.55

Weight (kDa)

5.55

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 27 - 277 3.8e-67 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 170
AciI CCGC 5 cut(s) 38, 159, 162, 254, 338
AclWI GGATC 2 cut(s) 337, 360
AcsI RAATTY 2 cut(s) 195, 913
AcuI CTGAAG 3 cut(s) 312, 329, 884
AcyI GRCGYC 3 cut(s) 5, 107, 156
AfaI GTAC 2 cut(s) 362, 774
AfiI CCNNNNNNNGG 1 cut(s) 100
AgsI TTSAA 4 cut(s) 216, 276, 464, 828
AjnI CCWGG 2 cut(s) 29, 607
AluBI AGCT 5 cut(s) 225, 397, 404, 682, 838
AluI AGCT 5 cut(s) 225, 397, 404, 682, 838
Alw21I GWGCWC 1 cut(s) 592
AlwI GGATC 2 cut(s) 337, 360
Ama87I CYCGRG 2 cut(s) 442, 708
AoxI GGCC 1 cut(s) 739
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 2 cut(s) 195, 913
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 5 cut(s) 34, 206, 632, 739, 758
AsuC2I CCSGG 2 cut(s) 443, 444
AsuHPI GGTGA 3 cut(s) 61, 115, 307
AvaI CYCGRG 2 cut(s) 442, 708
AvaII GGWCC 4 cut(s) 34, 206, 632, 758
BaeGI GKGCMC 1 cut(s) 175
BaeI ACNNNNGTAYC 2 cut(s) 498, 531
BanI GGYRCC 1 cut(s) 170
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 1 cut(s) 590
BccI CCATC 1 cut(s) 155
BciT130I CCWGG 2 cut(s) 31, 609
BciVI GTATCC 1 cut(s) 499
BcnI CCSGG 2 cut(s) 443, 444
BfaI CTAG 1 cut(s) 563
BfmI CTRYAG 2 cut(s) 333, 669
BfuI GTATCC 1 cut(s) 499
BisI GCNGC 3 cut(s) 159, 162, 579
BlsI GCNGC 3 cut(s) 160, 163, 580
BmcAI AGTACT 1 cut(s) 774
Bme1390I CCNGG 4 cut(s) 31, 443, 444, 609
Bme18I GGWCC 4 cut(s) 34, 206, 632, 758
BmeT110I CYCGRG 2 cut(s) 442, 708
BmgT120I GGNCC 5 cut(s) 34, 206, 632, 739, 758
BmiI GGNNCC 5 cut(s) 35, 36, 172, 721, 740
BmrFI CCNGG 4 cut(s) 31, 443, 444, 609
BmrI ACTGGG 1 cut(s) 652
BmsI GCATC 4 cut(s) 241, 253, 275, 703
BmuI ACTGGG 1 cut(s) 652
BpmI CTGGAG 1 cut(s) 738
BpuMI CCSGG 2 cut(s) 443, 444
BsaAI YACGTR 1 cut(s) 688
BsaHI GRCGYC 3 cut(s) 5, 107, 156
BsaJI CCNNGG 5 cut(s) 30, 240, 442, 443, 608
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse118I RCCGGY 1 cut(s) 722
Bse1I ACTGG 4 cut(s) 634, 647, 780, 889
Bse3DI GCAATG 1 cut(s) 573
BseBI CCWGG 2 cut(s) 31, 609
BseDI CCNNGG 5 cut(s) 30, 240, 442, 443, 608
BseGI GGATG 1 cut(s) 438
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 4 cut(s) 634, 647, 780, 889
BseRI GAGGAG 1 cut(s) 51
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 1 cut(s) 590
BshFI GGCC 1 cut(s) 741
BshNI GGYRCC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 592
BsiHKCI CYCGRG 2 cut(s) 442, 708
BsiSI CCGG 4 cut(s) 110, 230, 443, 723
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 20
BsnI GGCC 1 cut(s) 741
BsoBI CYCGRG 2 cut(s) 442, 708
Bsp1286I GDGCHC 2 cut(s) 175, 592
Bsp1407I TGTACA 1 cut(s) 360
Bsp143I GATC 4 cut(s) 312, 329, 352, 880
BspACI CCGC 5 cut(s) 38, 159, 162, 254, 338
BspANI GGCC 1 cut(s) 741
BspCNI CTCAG 1 cut(s) 151
BspLI GGNNCC 5 cut(s) 35, 36, 172, 721, 740
BspPI GGATC 2 cut(s) 337, 360
BspQI GCTCTTC 1 cut(s) 51
BspT107I GGYRCC 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 573
BsrFI RCCGGY 1 cut(s) 722
BsrGI TGTACA 1 cut(s) 360
BsrI ACTGG 4 cut(s) 634, 647, 780, 889
BssAI RCCGGY 1 cut(s) 722
BssECI CCNNGG 5 cut(s) 30, 240, 442, 443, 608
BssMI GATC 4 cut(s) 312, 329, 352, 880
BssNI GRCGYC 3 cut(s) 5, 107, 156
Bst2UI CCWGG 2 cut(s) 31, 609
Bst4CI ACNGT 1 cut(s) 636
Bst6I CTCTTC 2 cut(s) 51, 789
BstACI GRCGYC 3 cut(s) 5, 107, 156
BstAPI GCANNNNNTGC 2 cut(s) 170, 587
BstAUI TGTACA 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 688
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 2 cut(s) 138, 839
BstF5I GGATG 1 cut(s) 438
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 4 cut(s) 315, 332, 355, 883
BstMBI GATC 4 cut(s) 312, 329, 352, 880
BstMWI GCNNNNNNNGC 4 cut(s) 143, 170, 587, 713
BstNI CCWGG 2 cut(s) 31, 609
BstSCI CCNGG 4 cut(s) 29, 441, 442, 607
BstSFI CTRYAG 2 cut(s) 333, 669
BstSLI GKGCMC 1 cut(s) 175
BstSNI TACGTA 1 cut(s) 688
BstV1I GCAGC 1 cut(s) 590
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 1 cut(s) 741
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 1 cut(s) 438
BtsIMutI CAGTG 3 cut(s) 384, 627, 640
Cac8I GCNNGC 1 cut(s) 42
CfoI GCGC 1 cut(s) 88
Cfr10I RCCGGY 1 cut(s) 722
Cfr13I GGNCC 5 cut(s) 34, 206, 632, 739, 758
Cfr9I CCCGGG 1 cut(s) 442
CseI GACGC 2 cut(s) 115, 164
Csp6I GTAC 2 cut(s) 361, 773
CspCI CAANNNNNGTGG 2 cut(s) 736, 771
CviAII CATG 4 cut(s) 283, 622, 735, 791
CviQI GTAC 2 cut(s) 361, 773
DdeI CTNAG 2 cut(s) 138, 839
DpnI GATC 4 cut(s) 314, 331, 354, 882
DpnII GATC 4 cut(s) 312, 329, 352, 880
Eam1104I CTCTTC 2 cut(s) 51, 789
EarI CTCTTC 2 cut(s) 51, 789
Eco105I TACGTA 1 cut(s) 688
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 4 cut(s) 34, 206, 632, 758
Eco57I CTGAAG 3 cut(s) 312, 329, 884
Eco88I CYCGRG 2 cut(s) 442, 708
EcoO109I RGGNCCY 1 cut(s) 34
EcoRII CCWGG 2 cut(s) 29, 607
EcoRV GATATC 1 cut(s) 433
EcoT22I ATGCAT 1 cut(s) 268
FaeI CATG 4 cut(s) 286, 625, 738, 794
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 1 cut(s) 20
FatI CATG 4 cut(s) 282, 621, 734, 790
FauI CCCGC 2 cut(s) 45, 247
Fnu4HI GCNGC 3 cut(s) 159, 162, 579
FokI GGATG 1 cut(s) 425
Fsp4HI GCNGC 3 cut(s) 159, 162, 579
FspBI CTAG 1 cut(s) 563
GlaI GCGC 1 cut(s) 87
GluI GCNGC 3 cut(s) 159, 162, 579
GsuI CTGGAG 1 cut(s) 738
HaeIII GGCC 1 cut(s) 741
HapII CCGG 4 cut(s) 110, 230, 443, 723
HgaI GACGC 2 cut(s) 115, 164
HhaI GCGC 1 cut(s) 88
Hin1I GRCGYC 3 cut(s) 5, 107, 156
Hin1II CATG 4 cut(s) 286, 625, 738, 794
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HinfI GANTC 3 cut(s) 464, 472, 490
HpaII CCGG 4 cut(s) 110, 230, 443, 723
HphI GGTGA 3 cut(s) 61, 115, 307
Hpy188I TCNGA 3 cut(s) 141, 292, 309
Hpy188III TCNNGA 2 cut(s) 557, 710
Hpy99I CGWCG 3 cut(s) 10, 784, 787
HpyAV CCTTC 3 cut(s) 282, 466, 837
HpyCH4III ACNGT 1 cut(s) 636
HpyCH4IV ACGT 2 cut(s) 304, 687
HpyCH4V TGCA 5 cut(s) 266, 299, 578, 640, 655
HpyF10VI GCNNNNNNNGC 4 cut(s) 143, 170, 587, 713
HpyF3I CTNAG 2 cut(s) 138, 839
HpySE526I ACGT 2 cut(s) 304, 687
Hsp92I GRCGYC 3 cut(s) 5, 107, 156
Hsp92II CATG 4 cut(s) 286, 625, 738, 794
HspAI GCGC 1 cut(s) 86
KflI GGGWCCC 1 cut(s) 34
Kzo9I GATC 4 cut(s) 312, 329, 352, 880
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 2 cut(s) 340, 719
Lsp1109I GCAGC 1 cut(s) 590
LweI GCATC 4 cut(s) 241, 253, 275, 703
MaeI CTAG 1 cut(s) 563
MaeII ACGT 2 cut(s) 304, 687
MaeIII GTNAC 2 cut(s) 67, 103
MalI GATC 4 cut(s) 314, 331, 354, 882
MboI GATC 4 cut(s) 312, 329, 352, 880
MboII GAAGA 6 cut(s) 35, 38, 322, 427, 460, 806
MfeI CAATTG 1 cut(s) 95
MflI RGATCY 1 cut(s) 329
MhlI GDGCHC 2 cut(s) 175, 592
MluCI AATT 5 cut(s) 95, 195, 647, 828, 913
MlyI GAGTC 2 cut(s) 466, 484
MmeI TCCRAC 1 cut(s) 736
Mph1103I ATGCAT 1 cut(s) 268
MseI TTAA 3 cut(s) 78, 618, 911
MspI CCGG 4 cut(s) 110, 230, 443, 723
MspR9I CCNGG 4 cut(s) 31, 443, 444, 609
MunI CAATTG 1 cut(s) 95
MvaI CCWGG 2 cut(s) 31, 609
MwoI GCNNNNNNNGC 4 cut(s) 143, 170, 587, 713
NciI CCSGG 2 cut(s) 443, 444
NdeII GATC 4 cut(s) 312, 329, 352, 880
NlaIII CATG 4 cut(s) 286, 625, 738, 794
NlaIV GGNNCC 5 cut(s) 35, 36, 172, 721, 740
NmeAIII GCCGAG 1 cut(s) 265
NmuCI GTSAC 2 cut(s) 67, 103
NsiI ATGCAT 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 708
PciSI GCTCTTC 1 cut(s) 51
PfeI GAWTC 1 cut(s) 464
PkrI GCNGC 3 cut(s) 160, 163, 580
PleI GAGTC 2 cut(s) 466, 484
PpsI GAGTC 2 cut(s) 466, 484
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 34
Psp5II RGGWCCY 1 cut(s) 34
Psp6I CCWGG 2 cut(s) 29, 607
PspGI CCWGG 2 cut(s) 29, 607
PspN4I GGNNCC 5 cut(s) 35, 36, 172, 721, 740
PspPI GGNCC 5 cut(s) 34, 206, 632, 739, 758
PspPPI RGGWCCY 1 cut(s) 34
PsuI RGATCY 1 cut(s) 329
RsaI GTAC 2 cut(s) 362, 774
RsaNI GTAC 2 cut(s) 361, 773
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 3 cut(s) 78, 618, 911
SatI GCNGC 3 cut(s) 159, 162, 579
Sau3AI GATC 4 cut(s) 312, 329, 352, 880
Sau96I GGNCC 5 cut(s) 34, 206, 632, 739, 758
ScaI AGTACT 1 cut(s) 774
SchI GAGTC 2 cut(s) 466, 484
ScrFI CCNGG 4 cut(s) 31, 443, 444, 609
SduI GDGCHC 2 cut(s) 175, 592
SfaNI GCATC 4 cut(s) 241, 253, 275, 703
SfcI CTRYAG 2 cut(s) 333, 669
Sfr274I CTCGAG 1 cut(s) 708
SinI GGWCC 4 cut(s) 34, 206, 632, 758
SlaI CTCGAG 1 cut(s) 708
SmaI CCCGGG 1 cut(s) 444
SmlI CTYRAG 1 cut(s) 708
SmoI CTYRAG 1 cut(s) 708
SnaBI TACGTA 1 cut(s) 688
Sse9I AATT 5 cut(s) 95, 195, 647, 828, 913
SsiI CCGC 5 cut(s) 38, 159, 162, 254, 338
SspMI CTAG 1 cut(s) 563
StyD4I CCNGG 4 cut(s) 29, 441, 442, 607
TaaI ACNGT 1 cut(s) 636
TaiI ACGT 2 cut(s) 307, 690
TaqI TCGA 3 cut(s) 150, 435, 709
TasI AATT 5 cut(s) 95, 195, 647, 828, 913
TatI WGTACW 2 cut(s) 360, 772
TauI GCSGC 2 cut(s) 161, 164
TfiI GAWTC 1 cut(s) 464
Tru1I TTAA 3 cut(s) 78, 618, 911
Tru9I TTAA 3 cut(s) 78, 618, 911
TscAI CASTG 3 cut(s) 391, 634, 647
TseFI GTSAC 2 cut(s) 67, 103
TseI GCWGC 1 cut(s) 578
Tsp45I GTSAC 2 cut(s) 67, 103
TspDTI ATGAA 2 cut(s) 448, 807
TspGWI ACGGA 2 cut(s) 405, 800
TspMI CCCGGG 1 cut(s) 442
TspRI CASTG 3 cut(s) 391, 634, 647
VpaK11BI GGWCC 4 cut(s) 34, 206, 632, 758
XapI RAATTY 2 cut(s) 195, 913
XhoI CTCGAG 1 cut(s) 708
XmaI CCCGGG 1 cut(s) 442
XspI CTAG 1 cut(s) 563
ZrmI AGTACT 1 cut(s) 774
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.