pycom15g07850

Protein TIC 22, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012495
Physical Location & Seq
Reverse (-)
4840763 .. 4841988
1226 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 525 bp
ATGGGCTGGATTGTATTGGAAAACCATTCAGCTTTACTCGGCTCACGCACTGAGACGATGGAGTCCGCAAAGCCCTACCCGCGCTCAAACCCTCTGCTCTCGCTCTCCACCTTCATACACCAGCACTGCCTCCGCCTCGGCGCCGAGCTCTCCACCCGTTTCGAAGACACCAAGCGGCTCGCGGGAATCCTAGCCGGAAACTTCGCGCCGCTGGCGAGCAGGCGCATGTGTTCGGCTCCGGTTGCTCCGCCCTTCGCGTGGCTGGCTCAGAGCAATCACGCCCTGGCCGCTACCCTCAGCTCGGACCACGTGGCGAAGAGCCTGGCCGGCACGGCGGTGTATACGGTTAGCAATTCGAACAATGAGTTCGTGCTCATATCGATCCCAATGAGAGCAGGTCCATTGGATTGCTTTGCTTTCGGCAGGAAGACGCCGAAGCATTCCTCGCTCAAGTTCGATCGAGGAGAGAATTACGAAGTGCGGCTAAAGTTGTCCCGATTACTCTTGACCAGGTGTACATGCTGA

Protein Analysis

175

Amino Acids

19.09

Weight (kDa)

9.62

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 32 - 133 5e-21 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33350 AT4G33350
fragaria_vesca FvH4_2g34490
malus_domestica MD08G1102400.v1.1 MD15G1083800.v1.1
prunus_persica Prupe.1G436300_v2.0.a1
pyrus_communis pycom08g08320 pycom15g07850 pycom15g07890
rosa_chinensis RchiOBHm_Chr6g0307851
rosa_laevigata RLG00000010693
rosa_multiflora Rmu_sc0003252.1_g000014
rosa_roxburghii Rroxscaffold_7G00160820
rosa_rugosa Rorug06G0361400
rosa_samantha Rh6AG470200 Rh6BG481200 Rh6CG486500 Rh6DG472700
rosa_wichuraiana Rw6G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 61
Acc36I ACCTGC 1 cut(s) 386
AccB1I GGYRCC 1 cut(s) 140
AccI GTMKAC 1 cut(s) 341
AccII CGCG 4 cut(s) 82, 182, 206, 257
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 2 cut(s) 285, 324
AcvI CACGTG 1 cut(s) 310
AcyI GRCGYC 2 cut(s) 141, 431
AfaI GTAC 1 cut(s) 517
AfiI CCNNNNNNNGG 2 cut(s) 258, 301
AjnI CCWGG 3 cut(s) 282, 321, 509
AleI CACNNNNGTG 1 cut(s) 335
AluBI AGCT 3 cut(s) 32, 148, 300
AluI AGCT 3 cut(s) 32, 148, 300
Alw21I GWGCWC 2 cut(s) 150, 375
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 1 cut(s) 376
AoxI GGCC 2 cut(s) 285, 324
AspLEI GCGC 4 cut(s) 84, 143, 208, 225
AspS9I GGNCC 2 cut(s) 304, 398
AsuII TTCGAA 2 cut(s) 162, 356
AvaII GGWCC 2 cut(s) 304, 398
BanI GGYRCC 1 cut(s) 140
BanII GRGCYC 1 cut(s) 150
BbrPI CACGTG 1 cut(s) 310
BbsI GAAGAC 2 cut(s) 171, 434
Bbv12I GWGCWC 2 cut(s) 150, 375
BbvCI CCTCAGC 1 cut(s) 296
BccI CCATC 1 cut(s) 52
BceAI ACGGC 1 cut(s) 348
BciT130I CCWGG 3 cut(s) 284, 323, 511
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 1 cut(s) 144
BfuAI ACCTGC 1 cut(s) 386
BglI GCCNNNNNGGC 2 cut(s) 327, 332
BisI GCNGC 4 cut(s) 176, 209, 288, 482
BlsI GCNGC 4 cut(s) 177, 210, 289, 483
Bme1390I CCNGG 3 cut(s) 284, 323, 511
Bme18I GGWCC 2 cut(s) 304, 398
BmgT120I GGNCC 2 cut(s) 304, 398
BmiI GGNNCC 2 cut(s) 142, 237
BmrFI CCNGG 3 cut(s) 284, 323, 511
BpiI GAAGAC 2 cut(s) 171, 434
Bpu10I CCTNAGC 1 cut(s) 296
Bpu14I TTCGAA 2 cut(s) 162, 356
BpuEI CTTGAG 1 cut(s) 434
Bsa29I ATCGAT 1 cut(s) 380
BsaAI YACGTR 1 cut(s) 310
BsaHI GRCGYC 2 cut(s) 141, 431
BsaJI CCNNGG 2 cut(s) 136, 282
BsaWI WCCGGW 1 cut(s) 238
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 301
Bse118I RCCGGY 1 cut(s) 326
BseBI CCWGG 3 cut(s) 284, 323, 511
BseCI ATCGAT 1 cut(s) 380
BseDI CCNNGG 2 cut(s) 136, 282
BseLI CCNNNNNNNGG 2 cut(s) 258, 301
BseMII CTCAG 3 cut(s) 42, 281, 310
BseRI GAGGAG 1 cut(s) 477
Bsh1236I CGCG 4 cut(s) 82, 182, 206, 257
Bsh1285I CGRYCG 1 cut(s) 460
BshFI GGCC 2 cut(s) 287, 326
BshNI GGYRCC 1 cut(s) 140
BshVI ATCGAT 1 cut(s) 380
BsiEI CGRYCG 1 cut(s) 460
BsiHKAI GWGCWC 2 cut(s) 150, 375
BsiSI CCGG 3 cut(s) 195, 239, 327
BslFI GGGAC 1 cut(s) 478
BslI CCNNNNNNNGG 2 cut(s) 258, 301
BsmAI GTCTC 1 cut(s) 47
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 2 cut(s) 287, 326
Bsp119I TTCGAA 2 cut(s) 162, 356
Bsp1286I GDGCHC 2 cut(s) 150, 375
Bsp1407I TGTACA 1 cut(s) 515
Bsp143I GATC 2 cut(s) 381, 457
BspANI GGCC 2 cut(s) 287, 326
BspCNI CTCAG 3 cut(s) 43, 280, 309
BspDI ATCGAT 1 cut(s) 380
BspFNI CGCG 4 cut(s) 82, 182, 206, 257
BspLI GGNNCC 2 cut(s) 142, 237
BspMI ACCTGC 1 cut(s) 386
BspPI GGATC 1 cut(s) 376
BspQI GCTCTTC 1 cut(s) 311
BspT104I TTCGAA 2 cut(s) 162, 356
BspT107I GGYRCC 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 326
BsrGI TGTACA 1 cut(s) 515
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 2 cut(s) 136, 282
BssMI GATC 2 cut(s) 381, 457
BssNAI GTATAC 1 cut(s) 342
BssNI GRCGYC 2 cut(s) 141, 431
Bst1107I GTATAC 1 cut(s) 342
Bst2UI CCWGG 3 cut(s) 284, 323, 511
Bst4CI ACNGT 1 cut(s) 346
Bst6I CTCTTC 1 cut(s) 311
BstACI GRCGYC 2 cut(s) 141, 431
BstAUI TGTACA 1 cut(s) 515
BstBAI YACGTR 1 cut(s) 310
BstBI TTCGAA 2 cut(s) 162, 356
BstC8I GCNNGC 6 cut(s) 180, 213, 217, 221, 264, 328
BstDEI CTNAG 3 cut(s) 51, 267, 296
BstFNI CGCG 4 cut(s) 82, 182, 206, 257
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 4 cut(s) 84, 143, 208, 225
BstKTI GATC 2 cut(s) 384, 460
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 2 cut(s) 381, 457
BstMCI CGRYCG 1 cut(s) 460
BstMWI GCNNNNNNNGC 8 cut(s) 79, 212, 242, 263, 287, 327, 332, 445
BstNI CCWGG 3 cut(s) 284, 323, 511
BstNSI RCATGY 2 cut(s) 229, 522
BstSCI CCNGG 3 cut(s) 282, 321, 509
BstUI CGCG 4 cut(s) 82, 182, 206, 257
BstV2I GAAGAC 2 cut(s) 171, 434
BstZ17I GTATAC 1 cut(s) 342
Bsu15I ATCGAT 1 cut(s) 380
BsuRI GGCC 2 cut(s) 287, 326
BsuTUI ATCGAT 1 cut(s) 380
BtsI GCAGTG 1 cut(s) 124
BtsIMutI CAGTG 2 cut(s) 48, 124
BveI ACCTGC 1 cut(s) 386
Cac8I GCNNGC 6 cut(s) 180, 213, 217, 221, 264, 328
CfoI GCGC 4 cut(s) 84, 143, 208, 225
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 2 cut(s) 304, 398
ClaI ATCGAT 1 cut(s) 380
CseI GACGC 1 cut(s) 439
CsiI ACCWGGT 1 cut(s) 509
Csp6I GTAC 1 cut(s) 516
CviAII CATG 2 cut(s) 226, 519
CviQI GTAC 1 cut(s) 516
DdeI CTNAG 3 cut(s) 51, 267, 296
DinI GGCGCC 1 cut(s) 142
DpnI GATC 2 cut(s) 383, 459
DpnII GATC 2 cut(s) 381, 457
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 2 cut(s) 285, 324
Eam1104I CTCTTC 1 cut(s) 311
EarI CTCTTC 1 cut(s) 311
EciI GGCGGA 2 cut(s) 122, 237
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 2 cut(s) 304, 398
Eco53kI GAGCTC 1 cut(s) 148
Eco72I CACGTG 1 cut(s) 310
EcoICRI GAGCTC 1 cut(s) 148
EcoRII CCWGG 3 cut(s) 282, 321, 509
EcoT38I GRGCYC 1 cut(s) 150
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 2 cut(s) 229, 522
FaiI YATR 5 cut(s) 116, 227, 342, 377, 520
FaqI GGGAC 1 cut(s) 478
FatI CATG 2 cut(s) 225, 518
FauI CCCGC 2 cut(s) 87, 175
FblI GTMKAC 1 cut(s) 341
Fnu4HI GCNGC 4 cut(s) 176, 209, 288, 482
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 4 cut(s) 176, 209, 288, 482
FspBI CTAG 1 cut(s) 191
GlaI GCGC 4 cut(s) 83, 142, 207, 224
GluI GCNGC 4 cut(s) 176, 209, 288, 482
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 2 cut(s) 287, 326
HapII CCGG 3 cut(s) 195, 239, 327
HgaI GACGC 1 cut(s) 439
HhaI GCGC 4 cut(s) 84, 143, 208, 225
Hin1I GRCGYC 2 cut(s) 141, 431
Hin1II CATG 2 cut(s) 229, 522
Hin6I GCGC 4 cut(s) 82, 141, 206, 223
HinP1I GCGC 4 cut(s) 82, 141, 206, 223
HinfI GANTC 2 cut(s) 62, 186
HpaII CCGG 3 cut(s) 195, 239, 327
Hpy166II GTNNAC 2 cut(s) 342, 516
Hpy188I TCNGA 2 cut(s) 270, 304
Hpy188III TCNNGA 2 cut(s) 495, 505
Hpy8I GTNNAC 2 cut(s) 342, 516
HpyAV CCTTC 2 cut(s) 121, 262
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 1 cut(s) 309
HpyF10VI GCNNNNNNNGC 8 cut(s) 79, 212, 242, 263, 287, 327, 332, 445
HpyF3I CTNAG 3 cut(s) 51, 267, 296
HpySE526I ACGT 1 cut(s) 309
Hsp92I GRCGYC 2 cut(s) 141, 431
Hsp92II CATG 2 cut(s) 229, 522
HspAI GCGC 4 cut(s) 82, 141, 206, 223
KasI GGCGCC 1 cut(s) 140
KroI GCCGGC 1 cut(s) 326
KroNI GCCGGC 1 cut(s) 328
Kzo9I GATC 2 cut(s) 381, 457
LguI GCTCTTC 1 cut(s) 311
LmnI GCTCC 2 cut(s) 241, 250
MabI ACCWGGT 1 cut(s) 509
MaeI CTAG 1 cut(s) 191
MaeII ACGT 1 cut(s) 309
MalI GATC 2 cut(s) 383, 459
MboI GATC 2 cut(s) 381, 457
MboII GAAGA 3 cut(s) 176, 328, 439
MhlI GDGCHC 2 cut(s) 150, 375
MluCI AATT 2 cut(s) 352, 469
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 6 cut(s) 102, 140, 146, 305, 454, 455
MroNI GCCGGC 1 cut(s) 326
MslI CAYNNNNRTG 1 cut(s) 335
MspA1I CMGCKG 1 cut(s) 211
MspI CCGG 3 cut(s) 195, 239, 327
MspR9I CCNGG 3 cut(s) 284, 323, 511
Mva1269I GAATGC 1 cut(s) 439
MvaI CCWGG 3 cut(s) 284, 323, 511
MvnI CGCG 4 cut(s) 82, 182, 206, 257
MwoI GCNNNNNNNGC 8 cut(s) 79, 212, 242, 263, 287, 327, 332, 445
NaeI GCCGGC 1 cut(s) 328
NarI GGCGCC 1 cut(s) 141
NdeII GATC 2 cut(s) 381, 457
NgoMIV GCCGGC 1 cut(s) 326
NlaIII CATG 2 cut(s) 229, 522
NlaIV GGNNCC 2 cut(s) 142, 237
NmeAIII GCCGAG 3 cut(s) 18, 117, 169
NspI RCATGY 2 cut(s) 229, 522
NspV TTCGAA 2 cut(s) 162, 356
OliI CACNNNNGTG 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 311
PctI GAATGC 1 cut(s) 439
PdiI GCCGGC 1 cut(s) 328
PfeI GAWTC 1 cut(s) 186
PkrI GCNGC 4 cut(s) 177, 210, 289, 483
Ple19I CGATCG 1 cut(s) 460
PleI GAGTC 1 cut(s) 70
PluTI GGCGCC 1 cut(s) 144
PmaCI CACGTG 1 cut(s) 310
PmlI CACGTG 1 cut(s) 310
PpsI GAGTC 1 cut(s) 70
Ppu21I YACGTR 1 cut(s) 310
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 3 cut(s) 282, 321, 509
PspCI CACGTG 1 cut(s) 310
PspGI CCWGG 3 cut(s) 282, 321, 509
PspN4I GGNNCC 2 cut(s) 142, 237
PspPI GGNCC 2 cut(s) 304, 398
PvuI CGATCG 1 cut(s) 460
RsaI GTAC 1 cut(s) 517
RsaNI GTAC 1 cut(s) 516
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 311
SatI GCNGC 4 cut(s) 176, 209, 288, 482
Sau3AI GATC 2 cut(s) 381, 457
Sau96I GGNCC 2 cut(s) 304, 398
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 3 cut(s) 284, 323, 511
SduI GDGCHC 2 cut(s) 150, 375
SetI ASST 7 cut(s) 34, 113, 150, 302, 312, 400, 515
SexAI ACCWGGT 1 cut(s) 509
SfoI GGCGCC 1 cut(s) 142
SfuI TTCGAA 2 cut(s) 162, 356
SinI GGWCC 2 cut(s) 304, 398
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 2 cut(s) 352, 469
SspDI GGCGCC 1 cut(s) 140
SspMI CTAG 1 cut(s) 191
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 3 cut(s) 282, 321, 509
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 1 cut(s) 312
TaqI TCGA 5 cut(s) 162, 356, 380, 456, 460
TasI AATT 2 cut(s) 352, 469
TatI WGTACW 1 cut(s) 515
TauI GCSGC 4 cut(s) 178, 211, 290, 484
TfiI GAWTC 1 cut(s) 186
TscAI CASTG 2 cut(s) 55, 131
TspDTI ATGAA 1 cut(s) 103
TspRI CASTG 2 cut(s) 55, 131
VpaK11BI GGWCC 2 cut(s) 304, 398
XceI RCATGY 2 cut(s) 229, 522
XmiI GTMKAC 1 cut(s) 341
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.